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DiATOME DIAMOND KNIVES

Cryo Immuno Knife Handling and Use

1. Cryo Immuno Diamond Knife 2. Cryo Immuno Diamond Knives
  Setting the clearance angle.
Mounting the cryotrim diamond blade and the knife.
Distance of electrode-knife approx. 25mm.		   Tightening all set screws.

3a.

 

 cryo trim trimming blade
  Trimming with the cryotrim 45 diamond blade: ionizer on Pos. 10.

3b.

 cryo trim trimming blade
  Trimming with the cryotrim 90 diamond blade: ionizer on Pos. 10.
4. Start sectioning with the cryo immuno knife: ionizer on Pos. 10, distance of electrode - knife approx. 10mm.
After 2 - 3 sections: distance of electrode approx. 25mm.

4a.

 Cryo Immuno Diamond Knives

4b.

 Cryo Immuno Diamond Knives
  If sections lift up from the knife surface: reduce voltage.   If sections stick at the cutting edge: increase voltage.

5.

 Cryo Immuno Diamond Knives 6. Cryo Immuno Diamond Knives
  Manipulating sections with an eyelash: ionizer on Pos. 2.   Use special care when picking-up the sections:
Do not touch the cutting edge with any solid objects.
7. Remove knife from the chamber (before heating up!).

Cleaning Diamond KnifeCleaning

  • Bevel one of our polystyrol rods to an angle of approx. 60°.
  • Dip in ethanol 50% and shake off the excess.
    Gently run the rod across the cutting edge without applying lateral pressure.
  • If sections or debris dry on the knife edge:
    Place the knife in distilled water overnight. Clean the knife as described above.