Perfect Loop for Cryo Sectioning

1.		2.
	Pull the sections away from the cutting edge of the diamond knife with an eyelash.		Dip the LOOP in sucrose or sucrose/methyl-cellulose.
3.		4.
	Hold the LOOP above the cryo sections.		Touch the sections with the almost frozen sucrose or sucrose methyl-cellulose. Gently lift up the LOOP and bring back to room-temperature.
5.		6.
	Lower the LOOP onto a grid and lift up again.		Place the LOOP upside-down on a water surface to wash out the sucrose or sucrose methyl-cellulose and allow the grid to float free.

Please visit Electron Microscopy Sciences for additional information on the Perfect Loop.