Questions? 800-523-5874 | [email protected]
- Prepmaster™ Specimen Preparation Robot
- TEM Grids
- TEM Window Grids
- Omniprobe Nanomanipulation Systems
- K-kit Wet "Liquid" TEM Kit
- Specimen Mounts
- SEM Specimen Holders
- Index and Finder SEM Grids
- SEM for Forensics
- SEM Sample Preparation Station Materials
- Cryogenic Personal Protection Equipment
- Cryo Dewars & Flasks
- Cryogenic Grids & Accessories
- Cryogenic Vials & Racks
- Cooling Chambers & Ice Baths
- Prepmaster™ Specimen Preparation Robot
- Laboratory Microwave Ovens
- LYNX II Automated Tissue Processor
- EMS Poly III
- Microtomes
- Tissue Slicers
- Rapid Immersion Freezer
- Heaters & Chillers
- SEM Cooling Stage
- Glow Discharge Systems
- Sputter Coaters & Carbon Coaters
- Stages
- Freeze Dryers
- Critical Point Dryers
- Cryo-SEM Preparation System
- Specimen Transfer Systems
- Decontaminators
- Desiccators
- Centrifuges
- Dry Baths
- Stirrers, Hot Plates
- Vortexers & Magnetic Mixers
- Rotators & Rockers
- Ovens & Incubators
- Vibration Isolation
- Air Sampling
- Vacuum Pumps
EMS Technical Data Sheets
Lillie Modification of Mason's Trichrome Stain
EMS Catalog #: 26364-Series
Fixation:
Bouin’s (#15990-01) or Russell’s Zenker -contain no Mercury (#64123-05).
Sections:
Paraffin at 6µm.
Staining Procedures:
- Deparaffinize and hydrate to distilled water.
- If Zenker’s contain Mercury is used as fixative, remove Mercuric Chloride Crystal by placing in Lugol’s Iodine (#26364-07) or Gram’s Iodine (#26364-7A) for 15 minutes. Rinse in water and clear in Sodium Thiosulfate, 5% (#26364-08) for 3 minutes. Wash in water for 10 minutes or longer. If Russell’s Zenker (#64123-05) is used, go to step #3.
- Stain in Weigert’s Iron Hematoxylin Working* solution for 5 minutes. Wash in running water.
- * To prepare working solution, mix equal parts of Weigert’s Iron Hematoxylin A (#26364-02) and Weigert’s Iron Hematoxylin B (#26364-03).
- Stain in Biebrich Scarlet Solution (#26364-04) for 4 minutes. Rinse in water
- Mordant in Phosphomolybdic Acid-Phosphotungstic Acid (#26364-01) for 1 minute.
- Stain in Fast Green FCF Solution (#26364-05) for 4 minutes.
- Differentiate in Acetic Acid, 1% (#26364-06) for 1 minute.
- Carry through alcohol and dehydrate with acetone or absolute alcohol.
- Clear and mount in balsam saturated with salicylic acid.
Stain Results
| Nuclei | Black |
| Cytoplasm | Brown to Pink |
| Muscle | Red |
| Erythrocytes | Brilliant Scarlet |
| Collagen | Blue to Blue-Green |
| Myelinated Fibers | Red |
| Fibrin | Red to Pink |
References:
Clark, G.: Staining Procedures. Williams and Wilkins Co., Baltimore, 3rd ed., c. 1973, p. 59
Lillie, R.D., Arch. Path., 29:207, 1940.