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Electron Microscopy Sciences

EMS Academy Applications

arrow13The Aurion Immuno Gold Silver Staining Workshop

Three days of hands-on training for students, researchers, and microscopists who want to learn the most up to date theory and practice in Immuno Gold labeling.

Examples of the endless possibilities when doing Immuno Gold Staining

Immuno Gold Silver Staining   Pre-embedding Immuno Gold Labeling
Immuno Gold Silver Staining of E-cadherin
on a paraffin section of human skin.
Courtesy of R. Moella, Dept. of Exp. Path.,
EUR, The Netherlands
- Mouse monoclonal anti E-cadherin
- GAM lgG Ultra-Small
- Aurion R-Gent SE-LM
  Pre-embedding Immuno Gold Labeling
of Huntingtin Interacting Protein 3
in Mouse Brain using Aurion
GAR Fab-US and Aurion SEEM.
Courtesy of Ms Hong Yi,
Emory University,
Atlanta, GA
Immuno Gold Silver Staining   Immuno Gold Silver Staining
Immuno Gold Silver Staining of alpha-amylase on Lowicryl HM20 section of rat pancreas.
- Goat-anti-Rabbit, 15nm
- Ultra-small R-Gent (Dansher Method)

Facility

The EMS Microscopy Academy
Located in Hatfield, Pennsylvania, the Academy provides electron microscopy classes, workshops and training sessions for all fields of microscopy, including materials science and biological science.

Scope of Class

The objective of the course is to provide researchers with the opportunity to learn the theory and practice of Immuno Gold labeling. Participants will process their own samples under the expert guidance of our tutors, who are experts in Immuno Gold Silver Staining techniques.

During the workshop attendees will receive theory, including but not limited to immune detection, in situ hybridization, silver enhancement, as well as background issues. There will be time for practice, as well. Attendees will be able to work with their own specimens, as well as ones we will have prepared. A full review of incubation methods, testing of antigenicity and reactivity, complete principles of Immuno Gold labeling, and preparation of conjugates for EM and LM will be covered.

Format

Lecture, demonstration and hands-on practice, as well as round table discussion. Participants may bring their own samples to work on during lab time.

Note: Specimens need to be "ready to use", e.g., sections of chemically fixed cells on coverslips. For each combination of primary antibody and gold conjugate that you want to test during the workshop, you need at least 3, preferably 4, specimens. This enables you to test different dilutions of the primary antibody and to check the Immuno Gold/Silver reagents (negative control).

For pre-embedment immuno labeling, also include specimens that will be immuno labeled for evaluation on the light microscope. These specimens are used to see if the experiment is successful. During the workshop there is no time/no options to further process pre-embedment specimens for EM evaluation. Plastic embedment and sectioning needs to be done at your own facility.

Main Curriculum

  • The properties of gold particles and their protein conjugates
  • Theory underlying Immuno Gold labeling protocols
  • Silver enhancement of gold particles
  • Immuno Gold labeling on a variety of sample preparations of OLM
  • Immuno Gold labeling for EM
    • Pre-embedment Immuno Gold labeling using ultra small gold conjugates and silver enhancement
    • Post-embedment Immuno Gold labeling on plastic and ToKuyasu cryo sections using conventional colloidal conjugates and ultra small gold conjugates
    • Manual and automated Immuno Gold labeling
  • Pre/Post-embedment double Immuno Gold labeling
  • Background minimization in Immuno Gold labeling
  • Troubleshooting

Equipment

Hitachi TEM Inverted Light microscope Transmitted Light Microscope
Orbital Shakers Magnetic Stirrers PH Meter

Faculty

Peter van de Plas
Peter has a background in histology and immunocytochemistry and joined Aurion in 1991. During the pioneering phase of Aurion in the early nineties, he worked closely with Dr. Leunissen in founding a firm basis for Aurion. He contributed not only to the development of product applications, but also in designing the Aurion Immuno Gold Silver Staining workshop. He has been invited to many international microscopy conferences and workshops, and is especially experienced in providing hands-on training. Former workshop attendees and customers appreciate Peter for his technical support on and his thorough knowledge of the Immuno Gold silver staining techniques. The Aurion Immuno Gold workshops in Europe, Asia, Australia, and the US have all been fully attended and very well received.

Michael Kostrna
Michael was the program director of the Electron Microscopy Technician program at Madison Area Technical College and has more than more than 35 years in EM technical education and research experience. He has been training EM students for 29 years and has developed curricula and lab exercises for TEM, SEM, OLM, lab safety, introductory and advanced biological EM, EM, maintenance, and x-Ray microanalysis. He has worked with companies such as SC Johnson Polymer, Dow Chemicals, Io Genetics, Virent Technologies, ABS Global, NanoOnocology, and Microscopy Inovations, and in the process gained insight to the various applications of EM.

Al Coritz
Al has been doing Electron Microscopy for 38 years, beginning at the Yale School of Medicine and ending up on the commercial side with several key EM companies. His specialty is Cryo-techniques and Thin Film Technology: i.e. Freeze Fracture/Rotary Shadowing, High Pressure Freezing, and more. He is currently with Electron Microscopy Sciences where he has been the Technical Director for over 20 years.

View our schedule to see if this course is currently offered.

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