About Our Chemicals...
- Storage conditions of EACH chemical is noted in the box left of the catalog number.
- CODE: = Room Temperature
- For increased shelf life of room temperature stored chemicals, see equipment section for our complete line of Desiccator Cabinets. All chemicals must be kept away from direct sunlight.
- All specific gravities, unless otherwise specified are measured at water = 1
- All of the EMS stains are certified.
- For a chart of uses and applications of our Dyes & Stains, see pages 326-327
- EM Grade = ACS Reagent
Acetamido to Butyl
4-Acetamido-4'-Isothiocyanostilbene-2,2'-Disulfonic
Acid (SITS), Sodium Salt
C17H12N2O7S3Na2 F.W.
498.50 CAS #5-1023-76-8
SITS has been used as a vital stain, as a label for immunostaining, and
as a neuronal tracer. SITS is also a non-penetrating fluorescent marker
for erythrocyte membranes which acts as a potent inhibitor of anion transport.
For more information related to the preparation and characterization of
SITS, please refer to: Jakobsen, P. and Horobin, Richard W.(1989). Stain
Technology, Vol.64, No.6 pg.301-313.
| RT | 10030 | SITS, Disodium Salt | 250mg | 80.00 |
Acetate
Buffer
Please see listing in Prepared Buffers.
| RT | 11480 | 225 ml | 12.00 | ||
| RT | 11482 | 500 ml | 17.00 |
Acetone,
Reagent, A.C.S.
CH3COCH3 F.W. 58.08 CAS
#67-64-1
A.C.S. Specifications:
| Assay | Min 99.5% | |
| Aldehyde (as HCHO) | 0.002% | |
| Color | Max. APHA 10 | |
| Density (G/ml) at 25°C | Max. 0.7857 | |
| Titratable Acid | 0.0003 meq/g | |
| Titratable Base | 0.0006 meq/g | |
| Isopropyl Alchohol | 0.05% | |
| Methanol (CH3OH) | 0.05% | |
| Residue after Evaporation | 0.001% | |
| Solubilty in water | To pass test | |
| Substances Reducing Permanganate |
To pass test | |
| Water | 0.5% |
| RT | 10000 | 1qt. (946ml) | 13.00 | ||
| RT | 10010 | 4x1 qt. | 47.00 | ||
| RT | 10012 | 250ml | 6.00 | ||
| RT | 10014 | 4x250ml | 19.00 |
Acetate: Alcohol
Please see listing in Reagents for Histology: Fixing, Dehydrating, Clearing and Staining.
Acetone,
Glass Distilled CAS #67-64-74-1
U.V. Absorption - 1cm cell vs. H2O
| wavelength (nm) | 400 | 350 | 340 | 330 |
| A | 0.01 | 0.02 | 0.10 | 1.0 |
| RT | 10015 | 100ml | 12.00 | ||
| RT | 10016 | 4x100ml | 43.00 |
Acetonitrile,
Reagent, A.C.S.
CH3CN FW 41.05 CAS #75-05-8
A non-carcinogenic substitute for Propylene Oxide, used in tissueprocessing
for TEM. Good for infiltration with EMbed-812 or other Epon-812 substitutes.
Tarnowski, sB.I. et al, EMSA proceedings, Detroit, MI page 38 (1984)
| RT | 10020 | 450ml | 14.00 | ||
| RT | 10021 | 4x450ml | 50.00 |
Technical Tip
Acid Alcohols
An acidified alcohol solution for the differentiation of regressive stains.
| RT | 26071-01 | Acid Alcohol, 0.5% HCl in 70% Alcohol | 1 L | 34.00 | Add to Cart |
| RT | 26071-04 | Acid Alcohol, 0.5% HCl in 70% Alcohol | 4x1 L | 117.00 | Add to Cart |
| RT | 26072-01 | Acid Alcohol, 1% HCl in 70% Alcohol | 1 L | 29.00 | Add to Cart |
| RT | 26072-04 | Acid Alcohol, 1% HCl in 70% Alcohol | 4x1 L | 102.00 | Add to Cart |
| RT | 26073-01 | Acid Alcohol, 1% HCl in 95% Alcohol | 1 L | 38.00 | Add to Cart |
| RT | 26073-04 | Acid Alcohol, 1% HCl in 95% Alcohol | 4x1 L | 122.00 | Add to Cart |
Acid
Fuchsin, Certified, C.I. #42685
(Rubin S, Fuchsin S, Fuchsin Acid, Acid Magenta, Acid Violet
19)
C20H17N3Na2O9S3 F.W.
585.54 CAS #3244-88-0
Solubility: 0.053% Water; 1.21% Alcohol
| RT | 10035 | 25 g | 27.00 |
Acridine
Orange, C.I. #46005
(Basic Orange 14)
C17H2ON3 Cl F.W. 301.82 CAS
#65-61-2
Ultrastructural probes on DNA templates within human bone marrow and lymph node cells. Frenstar, J., Adv. Cell Mol. Biol., 3, 1(1974). Used to detect changes in DNA template activity within cells. Frenster, J. Cell Nucl., 1, 565 (1974) Fluorescent dye for cytochemistry. J. Histochem. Cytochem., 31, 737 ((1983) J. Lab. Med., 15 (3),180, (1984)
| RT | 10050 | 25g | 62.00 |
Adhesives and Mountants
Please see the Adhesive and Mountants section.
Agar,
Powder, Bacteriological Grade
(C12H18O9)x F.W. 3000-9000 CAS #9002-18-0
| Geling Temperature (1.5% in H2O) | ~35°C | |
| Gel Strength (1.5% gel) | 300g/cm2 |
Specifications:
| Acid insoluble ash | 0.5% | |
| Arsenic | 3ppm max | |
| Gelatin | To Past Test | |
| Heavy Metals | <0.004% | |
| Lead | <0.001% | |
| Total ash | <6.5% | |
| Water | <20% |
| RT | 10200 | 100g | 23.00 | ||
| RT | 10202 | 500g | 103.00 |
Agarose,
White, Fine Powder
C6H10O5·C6H8O4)n CAS
# 9012-36-6
For DNA Seperations
Specifications:
| Gel Strength | 1000g/cm2 | |
| Gel Point | 36-39°C | |
| Melting Point | 87-89°C | |
| EEO (-mr) | 0.20-0.26 | |
| Sulfate contents | 0.3% |
| RT | 10205 | 100g | 170.00 |

Agarose,
White, Fine Powder
CAS #9012-36-6
Low Gel Temperature
Specifications:
| Gel Strength | 480g/cm2 | |
| Gelling Point | 27-29°C | |
| Melting Point | 67-69°C | |
| EEO (-mr) | 0.10-0.15 | |
| Sulfate contents | 0.15% |
A 1% solution will remain liquid at 37°C for up to 24 hours. It will set to a firm gel at 25°C and will not remelt until temperatures exceed 65°C. This type of Agarose is also used for studies of cellular antibody production and the Jerne plaque assay of immune responses.
| RT | 10207 | 100g | 3904.00 |
Alcian
Blue 8GX, C.I. #74240 (Ingrain Blue I)
C56H68N16S4Cu F.W.
1298.88 CAS #12040-44-7
Dye Content: Approx. 50%
Solubility: Water-soluble
Used as a fixative: 0.5-2% Alcian Blue in Glutaraldehyde fixative
enhances the preservation and constrast of the cell coat and
intercellular substances.
Used as a stain: For staining cell surface materials and cartilage components.
Schofield, et al., Histochem J. 7, 139,1975
Used as a wetting agent: Sommer, J. R (1977). To cationize glass. J.Cell
Bio. 75, 245a. Alcian Blue and Ruthenium Red were used to study cartilage
ultrastructure. Thyberg, J., Lohmander, S., and Friberg, U., J. Ultrastruct.
Res., 45, 407 (1973).
| RT | 10350 | 25g | 200.00 |
Technical Tip
Alizarin
Red S, Certified, C.I. #58005
(Alizarin Camine, Mordant Red 3)
C14H7O7SNa.H2O F.W.
360.28 CAS #130-22-3
Solubility: 7.69% Water; 0.1% Alcohol.
Certified for Staining Bone. Stain for nervous tissue in small invertebrates, and for sections of nervous tissue. Lee, "The Microtomist's Vade-Mecum", 10th Ed., The Blakiston Co., Phila., 1937; Dawson, Stain Tech., 1, 123 (1926); Lundvall, Anat. Anz., 27, 521 (1905); 137. Use to differentiate bone from cartilage in mammalian embryos; used with Toluidine Blue. William, Stain Tech., 16, 23 (1941)
| RT | 10360 | 25g | 36.00 |
Aluminum Oxide, Powder, Reagent
(Alumina)
Al2O3 F.W. 101.96 CAS 1344-28-1
Specifications:
| Chloride: | 0.005% |
| Iron (Fe) | 0.03% |
| Substances not precipitated by NH4OH: | 0.75% |
| Loss on Ignition: | 5.0% |
| RT | 10400 | Aluminum Oxide | 500 g | 80.00 |
Amphibian Ringer Solution
Ingredients: NaCl; KCl; CaCl2·6H2O and Distilled water.
| RT | 11762-05 | Amphibian Ringer Solution | 500ml | 18.00 | |
| RT | 11762-10 | Amphibian Ringer Solution | 1 L | 28.00 |
Ammonium
Hydroxide, 28% Reagent, A.C.S.
NH4OH F.W. 35.05 CAS #1336-21-6
Specific Gravity: 0.91
Assay 28-30%
Specifications:
| Residue After Ignition | 0.002% | |
| Carbon Dioxide | 0.002% | |
| Chloride | 0.5 ppm | |
| Phosphate | 2 ppm | |
| Sulfate | 2 ppm | |
| Heavy Metals | 0.5 ppm | |
| Iron | 0.2 ppm |
| RT | 10600 | 450ml | 13.00 | ||
| RT | 10601 | 4x450ml | 47.00 |
Ammonium
Molybdate, Reagent, 99%
(Molybdic acid, ammonium salt)
(NH4)6MO7O24 ·4H2O F.W.
1235.86 CAS #12027-67-7
Used in the negative staining of viruses for TEM. Modifications
caused by uncoupling agents.
Muscatello, U., et al, J. Ultrastruct., Res., 52, 2 (1975)
| RT | 10500 | 100g | 37.00 |
Amyl
Acetate, Reagent, A.C.S.
CH3COOC5H11 FW 130.18 CAS
#628-63-7
Boiling Range: 140.0°C-150.0°C
Specific Gravity @ 25° C: 0.870-0.875
A.C.S. Specifications:
| Acidity (as CH3COOH) | 0.01% | |
| Non-volatile Matters | 0.01% | |
| Water (W/W) | 0.2 % |
| RT | 10800 | (Plastic coated btl.) (946ml) | 1qt | 42.00 | |
| RT | 10810 | 4x1qt | 158.00 |
Amyl
Acetate, Purified, Glass Distilled
CAS #628-63-7
| RT | 10815 | (Plastic coated btl.) | 100ml | 10.00 | |
| RT | 10816 | 4x100ml | 36.00 |
Aniline
Blue, Water Soluble, C.I. #42755
(Acid Blue 22; China Blue; Cotton Blue; Marine Blue V; Soluble
Blue 3M, 2R; Water Blue)
C32H25N3O9S3Na2 F.W.
737.74 CAS #28631-66-5
Solubility: 50% Water; 0.0% Alcohol; 4.5% Cellosolve; 7.8% Glycol;
0.0% Xylene
Used in connective tissue staining procedures to stain collagen.
Used in differential staining. Snodgress el al., Lab Invest.
26:329, 1972.
| RT | 10840 | 25g | 27.00 |
Antibody Dilution Buffer with Immunoglobulin- Free BSA
This ready-to-use Antibody Dilution Buffer is designed for the dilution of antibodies in all immunoassays, including immunofluorescence, IHC, ELISA, WB. This buffer contains immunoglobulin free BSA and does not contain phosphate, sodium azide, or mercury preservatives. It can be used for the dilution of all antibodies, including peroxidase, and antibodies to phophoproteins. However, it is not suitable for dilution of antibodies to S100 proteins. This buffer contains green food color.
Reagent Supplied: Ready to use buffer
Application: Dilute antibodies, primary or secondary as recommended in the protocol by the manufacturer of these products.
Storage: 2 – 8°C. Diluted antibodies can be stored at 2 – 8°C for several weeks, depending upon the stability of antibodies.
| 2–8°C | 25886-01 | Antibody Dilution Buffer, free BSA | 100 ml | 40.00 | |
| 2–8°C | 25886-05 | Antibody Dilution Buffer, free BSA | 500 ml | 99.00 |
Araldite
Resin
W.P.E. number included on each bottle.
Araldite
Embedding Kits
Please see listing in Embedding Kits.
| RT | 13900 | Araldite 502 Kit | kit | 40.00 | |
| RT | 13920 | Araldite 6005 Kit | kit | 42.00 | |
| RT | 13940 | Araldite-EMbed (Mollenhauer’s Kit) | kit | 61.00 |
Technical Tips
For Araldite RD-1 see BGE.
For Araldite RD-2, see BDE
Auramine
O, Certified, C.I. # 41000
(Pyoctanunum aureum; Basic Yellow 2; Pyoktanin Yellow; Canary
Yellow)
C17H21N3HCl·H2O F.W.
321.86 CAS # 2465-27-2
Solubility: 1.0% Water; 4.0% Alcohol; 1.2% Cellosolve; 1.75% Glycol; 0.05%
Xylene
| RT | 10850 | 25 g | 56.00 |
Azocarmine
G, Certified, C.I. #50085
(Acid red 101; Basalan Carmine GE; Rosinduline)
C28H17N3Na2O6S2 F.W.
601.57 CAS #25641-18-3
Solubility: 1.0% Water; 0.1% Alcohol; 1.75% Cellosolve; 4.5% Glycol; 0.01%
Xylene
| RT | 11150 | 5 g | 37.00 |
Azur
A, C.I. #52005(Methylene Azur A)
C14H14ClN3S F.W. 291.80 CAS
#531-53-3
Solubility: Water-soluble; Alcohol-soluble
Used in nuclear staining; A polychromatic blood stain.
| RT | 11240 | 10g | 80.00 |
Azur
B, C.I. #52010(Azur I; Methylene Azur B)
C15H16N3SBr FW 350.29 CAS #531-55-5
Solubility: Water-soluble; Alcohol-slightly soluble. An important
component of Giemsa's stain for blood protozoa. Used for staining
semi-thin sections of plant tissue.
Hoefert, L.L. (1968). Polychromatic stains for thin sections of Beta embedded
in epoxy resin. Stain Tech. 43:145
| RT | 11200 | 10g | 52.00 |
Azur
II, C.I. #52010/52015
(Azur B/Methylene Blue 1:1) CAS #37247-10-2
| 11210 | 10g | 38.00 |
Azur
II Eosin, C.I. #52010/52015/45380
(Azur B/Methylene Blue/Eosin 1:1:1) CAS #53092-85-6
| RT | 11220 | 10g | 53.00 |
Azur
Mixture Solution
Please see Giemsa Solution.
Basic
Fuchsin, Certified, C.I. #42510
(Rasaniline Chloride; Magenta; Basic Violet 14)
C20H19N3.HCl F.W. 337.86 CAS
#632-99-5
Berkowitz et al., (1968). Selective staining of nervous tissue
for light microscopy following preparation for electron microscopy.
J. Histochem. Cytochem. 16:808.
Sato, T., and Shamoto, M (1973). A simple rapid polychrome stain for epoxy-embedded
tissue. Stain Technol. 48:223.
| RT | 11260 | 25g | 80.00 |
Basic
Green 4, Certified, C.I. #42000
(Malachite Green Oxalate; Diamond Green B; BX or P Extra; Light
Green N, Solid Green O; Victoria Green B or WB)
2(C23H25N2).2(C2H2O4).C2O4 F.W.
927.03 CAS #2437-29-8
Solubility: 10.0% Water; 8.5% Alcohol; 5.5% Cellosolve; 7.0% Glycol; 0.0%
Xylene.
Used as a bacteria stain and a counterstain for botanical material.
| RT | 18100 | 25g | 38.00 |
Benzoin,
U.V. Catalyst
C6H5CH(OH)COC6H5 F.W.
212.25 CAS #579-44-2
m.p. 134-136°C
| RT | 11270 | 100g | 12.00 |
Benzoin
Ethyl Ether
(ethoxy phenylacetophenone)
C6H5COCH(CO2H5)C6H5 F.W.
240.31 CAS #574-09-4
m.p. 56-59°C
| RT | 11280 | 50g | 45.00 |
Benzoin
Methyl Ether, UV Catalyst
[alpha-Ethoxy-alpha-phenylacetophenone)
C6H5CH(OCH3)COC6H5 F.W.
226.28 CAS #3524-62-7
Assay.>98%
m.p. 47 - 49°C
| RT | 11290 | 10g | 18.00 |
(BDMA),
Benzyldimethylamine
N-Benzyl-N, N-Dimethylamine
C6H5CH2N(CH3)2 FW.
135.21 CAS #103-83-3
b.p. 177-180 °C
An amine accelerator for polymerization of epoxy resins.
A direct alternative to DMP-30 with a longer shelf life and better penetration.
Purity: 98%
| 11400 | 100ml | 8.00 | |||
| 11400-25* | 25ml | 3.00 |
Technical Tip
Use of Accelerators for Epoxy Resins
Biebrich
Scarlet, Water Soluble, Certified, C.I. #26905
(Bonceau BS; Acid Red 66; Croceine Scarlet; Double Scarlet BSF)
C22H14N4Na2O7S2 F.W.
556.4 9 CAS #4196-99-0
Solubility: 5.0% Water; 0.25% Alcohol; 1.5% Cellosolve; 1.5% Glycol; 0.0%
Xylene
| RT | 11265 | 25g | 20.00 |
BioBond;
Tissue Section Adhesive
Please see listing in Adhesives and Mountants.
Biomeda
Mounting Media
Please see listing in Adhesives and Mountants.
BioMount
Please see listing in Adhesives and Mountants.
Bismarck
Brown Y, Certified, C.I. #21000
(Basic Brown 1; Basic Brown G, GX, GXP; Vesuvine; Leather Brown;
Manchester Brown; Phenylene Brown)
C18H18N8·2HCl F.W. 419.32 CAS
#10114-58-6
Solubility: 1.5% Water; 3.0% Alcohol; 3.0% Cellosolve; 7.9% Glycol; 0.05%
Xylene
| RT | 11310 | 25g | 20.00 |
Bismuth
Carbonate, Basic, Purified
(Bismuth Subcarbonate)
Approx. (BiO)2CO3 CAS # 5892-10-4
Used for staining synapses.
Pfenninger, K., Sandri, C., Albert, K. and Eugster, C.H. (1969). Contribution
of the problem of structural organization of the presynaptic area. Brain
Res., 12:10
| RT | 11420 | 25g | 20.00 |
Bismuth
Stain Kit
Bismuth specifically stains Golgi beads and interchromatin granules
after fixation with glutaraldehyde, whereas it stains nucleoli,
basic protein, and biogenic amines after formaldehyde fixation.
Bismuth staining of sections is relatively nonspecific: it can
stain glycogen, lysosomes, ribosomes, polysaccharides, ferritin,
and DNA.
M.A. Hayat, "Basic Techniques for TEM" p. 184 (1986).
1. Specific En Bloc Staining (Locke & Huie, 1977)
Kit
contains: 10x5ml Solution A (Sodium tartrate, Sodium hydroxide
and Bismuth Subnitrate)
10x10ml solution B (.2M Triethanolamine-HCl buffer)
| RT | 11430 | Kit | 35.00 |
2. Enhancing Contrast in General (Riva, 1974)
Kit
contains: 5x10ml Solution A(Sodium tartrate in NaOH)
5x200mg Ingredient B (Bismuth subnitrate)
| RT | 11434 | Kit |

