4-Acetamido-4'-Isothiocyanostilbene-2,2'-Disulfonic Acid (SITS), Sodium Salt

C₁₇H₁₂N₂O₇S₃Na₂   F.W. 498.50   CAS #5-1023-76-8
SITS has been used as a vital stain, as a label for immunostaining, and as a neuronal tracer. SITS is also a non-penetrating fluorescent marker for erythrocyte membranes which acts as a potent inhibitor of anion transport. For more information related to the preparation and characterization of SITS, please refer to: Jakobsen, P. and Horobin, Richard W.(1989). Stain Technology, Vol.64, No.6 pg.301-313.

RT

10030

SITS, Disodium Salt

250mg

80.00

Acetate Buffer

Please see listing in Prepared Buffers.

RT	11480		225 ml	13.00
RT	11482		500 ml	18.00

Acetone, Reagent, A.C.S.

CH₃COCH₃   F.W. 58.08   CAS #67-64-1
A.C.S. Specifications:

Assay		Min 99.5%
Aldehyde (as HCHO)		0.002%
Color		Max. APHA 10
Density (G/ml) at 25°C		Max. 0.7857
Titratable Acid		0.0003 meq/g
Titratable Base		0.0006 meq/g
Isopropyl Alchohol		0.05%
Methanol (CH3OH)		0.05%
Residue after Evaporation		0.001%
Solubilty in water		To pass test
Substances Reducing Permanganate		To pass test
Water		0.5%

RT	10000		1qt. (946ml)	13.00
RT	10010		4x1 qt.	48.00
RT	10012		250ml	7.00
RT	10014		4x250ml	20.00

Acetate: Alcohol

Please see listing in Reagents for Histology: Fixing, Dehydrating, Clearing and Staining.

Acetone, Glass Distilled CAS #67-64-74-1

U.V. Absorption - 1cm cell vs. H₂O

wavelength (nm)	400	350	340	330
A	0.01	0.02	0.10	1.0

RT	10015		100ml	13.00
RT	10016		4x100ml	44.00

Acetonitrile, Reagent, A.C.S.

CH₃CN   FW 41.05   CAS #75-05-8
A non-carcinogenic substitute for Propylene Oxide, used in tissueprocessing for TEM. Good for infiltration with EMbed-812 or other Epon-812 substitutes. Tarnowski, sB.I. et al, EMSA proceedings, Detroit, MI page 38 (1984)

RT	10020		450ml	68.00
RT	10021		4x450ml	245.00

Technical Tip

Use of Acetonitrile

Acid Alcohols

An acidified alcohol solution for the differentiation of regressive stains.

Acid Alcohol, 0.5% HCl in 70% Alcohol	26071
Acid Alcohol, 1% HCl in 70% Alcohol	26072

RT	26071-01	Acid Alcohol, 0.5% HCl in 70% Alcohol	1 L	34.00	Add to Cart
RT	26071-04	Acid Alcohol, 0.5% HCl in 70% Alcohol	4x1 L	117.00	Add to Cart
RT	26072-01	Acid Alcohol, 1% HCl in 70% Alcohol	1 L	29.00	Add to Cart
RT	26072-04	Acid Alcohol, 1% HCl in 70% Alcohol	4x1 L	102.00	Add to Cart
RT	26073-01	Acid Alcohol, 1% HCl in 95% Alcohol	1 L	38.00	Add to Cart
RT	26073-04	Acid Alcohol, 1% HCl in 95% Alcohol	4x1 L	122.00	Add to Cart

Acid Fuchsin, Certified, C.I. #42685

(Rubin S, Fuchsin S, Fuchsin Acid, Acid Magenta, Acid Violet 19)
C₂₀H₁₇N₃Na₂O₉S₃   F.W. 585.54   CAS #3244-88-0
Solubility: 0.053% Water; 1.21% Alcohol

RT

10035

25 g

27.00

Acridine Orange, C.I. #46005

(Basic Orange 14)
C₁₇H₂ON₃ Cl   F.W. 301.82   CAS #65-61-2

Ultrastructural probes on DNA templates within human bone marrow and lymph node cells. Frenstar, J., Adv. Cell Mol. Biol., 3, 1(1974). Used to detect changes in DNA template activity within cells. Frenster, J. Cell Nucl., 1, 565 (1974) Fluorescent dye for cytochemistry. J. Histochem. Cytochem., 31, 737 ((1983) J. Lab. Med., 15 (3),180, (1984)

RT

10050

25g

62.00

Adhesives and Mountants

Please see the Adhesive and Mountants section.

Agar, Powder, Bacteriological Grade

(C₁₂H₁₈O₉)x   F.W. 3000-9000   CAS #9002-18-0

Geling Temperature (1.5% in H2O)		~35°C
Gel Strength (1.5% gel)		300g/cm2

Specifications:

Acid insoluble ash		0.5%
Arsenic		3ppm max
Gelatin		To Past Test
Heavy Metals		<0.004%
Lead		<0.001%
Total ash		<6.5%
Water		<20%

RT	10200		100g	23.00
RT	10202		500g	103.00

Agarose, White, Fine Powder

C₆H₁₀O₅·C₆H₈O₄)_n   CAS # 9012-36-6
For DNA Seperations

Specifications:

Gel Strength		1000g/cm2
Gel Point		36-39°C
Melting Point		87-89°C
EEO (-mr)		0.20-0.26
Sulfate contents		0.3%

RT

10205

100g

170.00

Agarose, White, Fine Powder

CAS #9012-36-6
Low Gel Temperature

Specifications:

Gel Strength		480g/cm2
Gelling Point		27-29°C
Melting Point		67-69°C
EEO (-mr)		0.10-0.15
Sulfate contents		0.15%

A 1% solution will remain liquid at 37°C for up to 24 hours. It will set to a firm gel at 25°C and will not remelt until temperatures exceed 65°C. This type of Agarose is also used for studies of cellular antibody production and the Jerne plaque assay of immune responses.

RT

10207

100g

390.00

Alcian Blue 8GX, C.I. #74240 (Ingrain Blue I)

C₅₆H₆₈N₁₆S₄Cu   F.W. 1298.88   CAS #12040-44-7
Dye Content: Approx. 50%
Solubility: Water-soluble

Used as a fixative: 0.5-2% Alcian Blue in Glutaraldehyde fixative enhances the preservation and constrast of the cell coat and intercellular substances.
Used as a stain: For staining cell surface materials and cartilage components. Schofield, et al., Histochem J. 7, 139,1975
Used as a wetting agent: Sommer, J. R (1977). To cationize glass. J.Cell Bio. 75, 245a. Alcian Blue and Ruthenium Red were used to study cartilage ultrastructure. Thyberg, J., Lohmander, S., and Friberg, U., J. Ultrastruct. Res., 45, 407 (1973).

RT

10350

25g

200.00

Technical Tip

Purification of Alcian Blue

Alizarin Red S, Certified, C.I. #58005

(Alizarin Camine, Mordant Red 3)
C₁₄H₇O₇SNa^.H₂O   F.W. 360.28   CAS #130-22-3
Solubility: 7.69% Water; 0.1% Alcohol.

Certified for Staining Bone. Stain for nervous tissue in small invertebrates, and for sections of nervous tissue. Lee, "The Microtomist's Vade-Mecum", 10th Ed., The Blakiston Co., Phila., 1937; Dawson, Stain Tech., 1, 123 (1926); Lundvall, Anat. Anz., 27, 521 (1905); 137. Use to differentiate bone from cartilage in mammalian embryos; used with Toluidine Blue. William, Stain Tech., 16, 23 (1941)

RT

10360

25g

36.00

Aluminum Oxide, Powder, Reagent (Alumina)

Al₂O₃ F.W. 101.96 CAS 1344-28-1

Specifications:

Chloride:	0.005%
Iron (Fe)	0.03%
Substances not precipitated by NH4OH:	0.75%
Loss on Ignition:	5.0%

RT

10400

Aluminum Oxide

500 g

80.00

Amphibian Ringer Solution

Ingredients: NaCl; KCl; CaCl₂·6H₂O and Distilled water.

RT	11762-05	Amphibian Ringer Solution	500ml	19.00
RT	11762-10	Amphibian Ringer Solution	1 L	29.00

Ammonium Hydroxide, 28% Reagent, A.C.S.

NH₄OH   F.W. 35.05   CAS #1336-21-6
Specific Gravity: 0.91
Assay 28-30%

Specifications:

Residue After Ignition		0.002%
Carbon Dioxide		0.002%
Chloride		0.5 ppm
Phosphate		2 ppm
Sulfate		2 ppm
Heavy Metals		0.5 ppm
Iron		0.2 ppm

RT	10600		450ml	14.00
RT	10601		4x450ml	52.00

Ammonium Molybdate, Reagent, 99%

(Molybdic acid, ammonium salt)
(NH₄)₆MO₇O₂₄ ·4H₂O   F.W. 1235.86   CAS #12027-67-7

Used in the negative staining of viruses for TEM. Modifications caused by uncoupling agents.
Muscatello, U., et al, J. Ultrastruct., Res., 52, 2 (1975)

RT

10500

100g

37.00

Amyl Acetate, Reagent, A.C.S.

CH₃COOC₅H₁₁   FW 130.18   CAS #628-63-7
Boiling Range: 140.0°C-150.0°C
Specific Gravity @ 25° C: 0.870-0.875
A.C.S. Specifications:

Acidity (as CH3COOH)		0.01%
Non-volatile Matters		0.01%
Water (W/W)		0.2 %

RT	10800	(Plastic coated btl.) (946ml)	1qt	42.00
RT	10810		4x1qt	158.00

Amyl Acetate, Purified, Glass Distilled

CAS #628-63-7

RT	10815	(Plastic coated btl.)	100ml	10.00
RT	10816		4x100ml	36.00

Aniline Blue, Water Soluble, C.I. #42755

(Acid Blue 22; China Blue; Cotton Blue; Marine Blue V; Soluble Blue 3M, 2R; Water Blue)
C₃₂H₂₅N₃O₉S₃Na₂   F.W. 737.74   CAS #28631-66-5

Solubility: 50% Water; 0.0% Alcohol; 4.5% Cellosolve; 7.8% Glycol; 0.0% Xylene
Used in connective tissue staining procedures to stain collagen. Used in differential staining. Snodgress el al., Lab Invest. 26:329, 1972.

RT

10840

25g

27.00

Antibody Dilution Buffer with Immunoglobulin- Free BSA

This ready-to-use Antibody Dilution Buffer is designed for the dilution of antibodies in all immunoassays, including immunofluorescence, IHC, ELISA, WB. This buffer contains immunoglobulin free BSA and does not contain phosphate, sodium azide, or mercury preservatives. It can be used for the dilution of all antibodies, including peroxidase, and antibodies to phophoproteins. However, it is not suitable for dilution of antibodies to S100 proteins. This buffer contains green food color.

Reagent Supplied: Ready to use buffer

Application: Dilute antibodies, primary or secondary as recommended in the protocol by the manufacturer of these products.

Storage: 2 – 8°C. Diluted antibodies can be stored at 2 – 8°C for several weeks, depending upon the stability of antibodies.

2–8°C	25886-01	Antibody Dilution Buffer, free BSA	100 ml	40.00
2–8°C	25886-05	Antibody Dilution Buffer, free BSA	500 ml	99.00

Araldite Resin

W.P.E. number included on each bottle.

Araldite 502	10900
Araldite 506	10910
Araldite 6005	10920
Araldite 507	10930

 

RT	10900	Araldite 502; CAS #84-74-2, Specific Gravity: 1.13	450ml	14.00
RT	10910	Araldite 506; CAS #25068-38-6, Specific Gravity: 1.13	450ml	14.00
RT	10920	Araldite 6005; CAS #3101-60-8, Specific Gravity: 1.16	450ml	14.00
RT	10930	Araldite 507; CAS #26447-14-3, Specific Gravity: 1.14	450ml	14.00

Araldite Embedding Kits

Please see listing in Embedding Kits.

RT	13900	Araldite 502 Kit	kit	40.00
RT	13920	Araldite 6005 Kit	kit	42.00
RT	13940	Araldite-EMbed (Mollenhauer’s Kit)	kit	61.00

Technical Tips

For Araldite RD-1 see BGE.
For Araldite RD-2, see BDE

Auramine O, Certified, C.I. # 41000

(Pyoctanunum aureum; Basic Yellow 2; Pyoktanin Yellow; Canary Yellow)
C₁₇H₂₁N₃HCl·H₂O   F.W. 321.86   CAS # 2465-27-2
Solubility: 1.0% Water; 4.0% Alcohol; 1.2% Cellosolve; 1.75% Glycol; 0.05% Xylene

RT

10850

25 g

56.00

Azocarmine G, Certified, C.I. #50085

(Acid red 101; Basalan Carmine GE; Rosinduline)
C₂₈H₁₇N₃Na₂O₆S₂   F.W. 601.57   CAS #25641-18-3
Solubility: 1.0% Water; 0.1% Alcohol; 1.75% Cellosolve; 4.5% Glycol; 0.01% Xylene

RT

11150

5 g

37.00

Azur A, C.I. #52005(Methylene Azur A)

C₁₄H₁₄ClN₃S   F.W. 291.80   CAS #531-53-3
Solubility: Water-soluble; Alcohol-soluble
Used in nuclear staining; A polychromatic blood stain.

RT

11240

10g

80.00

Azur B, C.I. #52010(Azur I; Methylene Azur B)

C₁₅H₁₆N₃SBr   FW 350.29   CAS #531-55-5

Solubility: Water-soluble; Alcohol-slightly soluble. An important component of Giemsa's stain for blood protozoa. Used for staining semi-thin sections of plant tissue.
Hoefert, L.L. (1968). Polychromatic stains for thin sections of Beta embedded in epoxy resin. Stain Tech. 43:145

RT

11200

10g

52.00

Azur II, C.I. #52010/52015

(Azur B/Methylene Blue 1:1)   CAS #37247-10-2

11210

10g

38.00

Azur II Eosin, C.I. #52010/52015/45380

(Azur B/Methylene Blue/Eosin 1:1:1)   CAS #53092-85-6

RT

11220

10g

53.00

Azur Mixture Solution

Please see Giemsa Solution.

Basic Fuchsin, Certified, C.I. #42510

(Rasaniline Chloride; Magenta; Basic Violet 14)
C₂₀H₁₉N₃^.HCl   F.W. 337.86   CAS #632-99-5

Berkowitz et al., (1968). Selective staining of nervous tissue for light microscopy following preparation for electron microscopy.
J. Histochem. Cytochem. 16:808.
Sato, T., and Shamoto, M (1973). A simple rapid polychrome stain for epoxy-embedded tissue. Stain Technol. 48:223.

RT

11260

25g

80.00

Basic Green 4, Certified, C.I. #42000

(Malachite Green Oxalate; Diamond Green B; BX or P Extra; Light Green N, Solid Green O; Victoria Green B or WB)
2(C₂₃H₂₅N₂)^.2(C₂H₂O₄)^.C₂O₄   F.W. 927.03   CAS #2437-29-8
Solubility: 10.0% Water; 8.5% Alcohol; 5.5% Cellosolve; 7.0% Glycol; 0.0% Xylene.
Used as a bacteria stain and a counterstain for botanical material.

RT

18100

25g

39.00

Benzoin, U.V. Catalyst

C₆H₅CH(OH)COC₆H₅   F.W. 212.25   CAS #579-44-2
m.p. 134-136°C

RT

11270

100g

12.00

Benzoin Ethyl Ether

(ethoxy phenylacetophenone)
C₆H₅COCH(CO₂H₅)C₆H₅   F.W. 240.31   CAS #574-09-4
m.p. 56-59°C

RT

11280

50g

45.00

Benzoin Methyl Ether, UV Catalyst

[alpha-Ethoxy-alpha-phenylacetophenone)
C₆H₅CH(OCH₃)COC₆H₅   F.W. 226.28   CAS #3524-62-7
Assay.>98%
m.p. 47 - 49°C

RT

11290

10g

18.00

(BDMA), Benzyldimethylamine

N-Benzyl-N, N-Dimethylamine
C₆H₅CH₂N(CH₃)₂   FW. 135.21   CAS #103-83-3
b.p. 177-180 °C
An amine accelerator for polymerization of epoxy resins.
A direct alternative to DMP-30 with a longer shelf life and better penetration.
Purity: 98%

	11400		100ml	8.00
	11400-25*		25ml	4.00

Technical Tip

Use of Accelerators for Epoxy Resins

Biebrich Scarlet, Water Soluble, Certified, C.I. #26905

(Bonceau BS; Acid Red 66; Croceine Scarlet; Double Scarlet BSF)
C₂₂H₁₄N₄Na₂O₇S₂   F.W. 556.4   9 CAS #4196-99-0
Solubility: 5.0% Water; 0.25% Alcohol; 1.5% Cellosolve; 1.5% Glycol; 0.0% Xylene

RT

11265

25g

20.00

BioBond; Tissue Section Adhesive

Please see listing in Adhesives and Mountants.

Biomeda Mounting Media

Please see listing in Adhesives and Mountants.

BioMount

Please see listing in Adhesives and Mountants.

Bismarck Brown Y, Certified, C.I. #21000

(Basic Brown 1; Basic Brown G, GX, GXP; Vesuvine; Leather Brown; Manchester Brown; Phenylene Brown)
C₁₈H₁₈N₈·2HCl   F.W. 419.32   CAS #10114-58-6
Solubility: 1.5% Water; 3.0% Alcohol; 3.0% Cellosolve; 7.9% Glycol; 0.05% Xylene

RT

11310

25g

20.00

Bismuth Carbonate, Basic, Purified

(Bismuth Subcarbonate)
Approx. (BiO)₂CO₃   CAS # 5892-10-4
Used for staining synapses.
Pfenninger, K., Sandri, C., Albert, K. and Eugster, C.H. (1969). Contribution of the problem of structural organization of the presynaptic area. Brain Res., 12:10

RT

11420

25g

20.00

Bismuth Stain Kit

Bismuth specifically stains Golgi beads and interchromatin granules after fixation with glutaraldehyde, whereas it stains nucleoli, basic protein, and biogenic amines after formaldehyde fixation. Bismuth staining of sections is relatively nonspecific: it can stain glycogen, lysosomes, ribosomes, polysaccharides, ferritin, and DNA.
M.A. Hayat, "Basic Techniques for TEM" p. 184 (1986).

1. Specific En Bloc Staining (Locke & Huie, 1977)

Kit contains: 10x5ml Solution A (Sodium tartrate, Sodium hydroxide and Bismuth Subnitrate)
10x10ml solution B (.2M Triethanolamine-HCl buffer)

RT

11430

Kit

35.00

2. Enhancing Contrast in General (Riva, 1974)

Kit contains: 5x10ml Solution A(Sodium tartrate in NaOH)
5x200mg Ingredient B (Bismuth subnitrate)

RT

11434

Kit

22.00

3. Staining of Mucosubstances & Polysaccharides (Ainsworth et al., 1972)

Kit contains: 10x10ml Solution A (Periodic acid, Ethanol, Sodium acetate)
10x10ml Solution B (Sodium tartrate, NaOH, Bismuth subnitrate)

RT

11436

Kit

40.00

Bismuth Subnitrate, Crystalline

(Bismuth Nitrate, Basic; Bismuth Oxynitrate Hydrate)
4BiNO₃(OH₂)^.BiO(OH)   F.W. 1461.99   CAS #1304-85-4
Bismuth staining for light and electron microscopy.
M .Locke and P. Huie, Tissue & Cell, 9, 347 (1977); G.L. Brown & M. Locke, ibd. 10, 365 (1978)

RT

11450

25g

22.00

Borax Tetra, Sodium Borate, Reagent, A.C.S.

(Sodium Tetraborate, Decahydrate)
Na₂B₄O₇ ^.10H₂O   FW. 381.37   CAS #1303-96-4
A.C.S. Specifications

Assay		99.5-105%
Calcium		0.005%
Chloride		0.001%
Insoluble Matter		0.005%
Iron (Fe)		5ppm
pH of 0.01M Solution @ 25° C		9.15-9.20
Other Heavy Metals (as Pb)		0.001%
Phosphate (PO4 )		0.001%
Sulfate (S04)		0.005%

RT

21130

500g

20.00

Boric Acid, Reagent, Powder, A.C.S.

H₃BO₃   FW 61.83   CAS #10043-35-3
Assay 99.95% minimum.
A buffer component in Tris-EDTA-Borate buffer. Also used in Silver-Methenamine buffer. Swift, J.A. (1968). The Electron histochemistry of cystine-containing proteins in thin transverse sections of human hair. J.R. Microsc. Soc. 88,449.

RT

11460

500g

44.00

Bouin's Fixative

Please see listing in the Fixatives section.

Brilliant Cresyl Blue, Certified, C.I.#51010

(Brilliant Blue C; Cresyl Blue 2RN)
C₁₇H₂₁N₄OCl   F.W. 332.84   CAS #10127-36-3
Solubility: 3.0% Water; 2.6% Alcohol; 2.25% Cellosolve; 8.0% Glycol; 9.5% Xylene

RT

11465

10g

52.00

Bromophenol Blue, Reagent, A.C.S.

(3',3",5 '5"-Tetrabromophenol-sulfonthalein)
C₁₉H₁₀O₅SBr₄   F.W. 669.97   CAS #115-39-9

	11470		5g	20.00
	11471		25g	59.00

Buffers; Prepared

Please see our complete listing of Prepared Buffers

1,4-Butanediol Diglycidyl Ether;

(Araldite RD-2)
C₁₀H₁₈O₄   F.W. 202.2   CAS #2425-79-8
Approx. 70%
Specific Gravity: 1.10

RT

11800

100ml

40.00

2-Butoxy Ethanol

(Butyl Cellosolve®; Ethylene Glycol Monobutyl Ether)
CH₃(CH₂)₃OCH₂CH₂OH   F.W. 118.18   CAS #111-76-2
Specific Gravity: 0.90
b.p. 169°C

RT

11820

450ml

18.00

Butvar B-98 Powder

CAS #63148-65-2
(Terpolymer of polyvinyl butyl, polyvinyl alcohol & polyvinyl acetate)
Polyvinyl butyl resin for thin film support. Stronger than Formvar and more sticky, resulting in a firm attachment for slotted or meshed grids. The standard solution is 0.25% Butvar B-98 in Chloroform. Handley, D.A., et. al., Ultramicroscopy, 4,479 (1979).

RT

11850

100g

9.00

Butvar B-98 Solution

Stock solution : 1.0% in Chloroform.

RT

11860

100ml

12.00

Technical Tip

Tips for Butvar B-98 Support Films

n-Butyl Acetate, Reagent, A.C.S.

CH₃COO(CH₂)₃CH₃   F.W. 116.16   CAS #123-86-4
b.p . 127°C
Specific Gravity: 0.88

RT

11900

450ml

20.00

n-Butyl Alcohol, Reagent, A.C.S.

(Butanol)
CH₃CH₂CH₂CH₂OH   F.W. 74.12   CAS # 71-36-3

Specific Gravity:		0.81
Assay		99.4%
Residue after evaporation		0.005%
Titratable acid		0.0008meq/g
Butyl Ether		0.2%
Water		0.1%

RT	11920		450ml	20.00
RT	11921		4x450ml	72.00

Technical Tip

Preservation of Phospholipids

(BGE), n-Butyl Glycidyl Ether, (Araldite RD-1)

(Butoxymethyl oxirane)
C₇H₁₄O₂   F.W. 130.19   CAS #2426-08-6
b.p. 166-168°C
Specific Gravity: 0.912

RT

12000

100ml

7.00

n-Butyl Methacrylate, Monomer

CH₂C(CH₃)CO₂CH₂CH₂CH₂CH₃   F.W. 142.20   CAS #97-88-1
b.p. 162-164°C
Stabilized with 10ppm MEHQ. (Hydroquinone, Mononethylether)

RT

12100

225ml

15.00

Buffers