Aron Alpha® -Ethyl Ultra Speed Adhesive (Quickbond)

Aron Alpha , type 200 is a one component, room temperature curing structural adhesive composed mainly of alpha cyanoacrylate monomer in a liquid state. When this monomer is applied in a single thin coating to the bonding surface of the material, it instantly solidifies through polymerization to produce an extra high strength, colorless bond with virtually no shrinkage. Aron Alpha bonds almost any nonporous material except fluorocarbons. Solvents such as gasoline, propane, oil, alcohol or benzene do not affect resinified Aron Alpha. The adhesive itself contains no solvents. The Aron Alpha bond has a high tensile strength but a relatively lower shear strength. Aron Alpha is not a gap filler. Good surface contact is essential. Meets MIL-A-46050 C. Ideal for the bonding of tissues to a tissue-slicer, tissue block or to a blank block etc.

Physical Properties of Type 200

Before use (Liquid state)

Refractive Index, N20°C/D	.1.4363
Viscosity @25°C (Brookfield)	2
Specific Gravity, 20°C/4°C	1.050
Boiling Point, 5mmHg, °F	140-144
Melting Point (°F)	-130
Flash Point (°F)	181
Solubility	acetone, MEK, benzene, toluene, ethylacetate, nitromethane

After use (Bonded state)

Solubility Paramete	10.72
Softening Temp. (Viscat State), (°F)	293
Melting Point (°F)	392 – 406
Specific Gravity, 20°C / 4°C	1.2483
Refractive Index, N20°C/D	1.4870
Electric resistance	10 11-13
Solubility	acetone, nitromethane

RT

72588

Quick-Bond - Aron Alpha 200

5x2g/bx

16.50

Biobond; Tissue Section Adhesive

Having prepared the tissue specimen for immunolabeling it is then imperative to perform the incubations with a protocol designed to maximize the specific signal and minimize the background. Some incubation conditions may cause the tissue sections to be removed from the glass slide. Typical tissue section adhesives such as poly-L-lysine, Elmer's glue, chrome alum, etc... are not suitable for use with immunogold labeling because of the increased background caused by the attraction of gold particles to the adhesive on the slide.

Furthermore, the surface of glass slides is uneven and it is activated by the silicon tetrahedral structure. It, therefore, provides active sites for absorbtion of proteins or reactions with chemicals and reagents. Thus, it is important to minimize this possibility by coating the surface with a material that is of low reactivity towards reagents. BIOBOND produces a very strong adhesion between the glass slide and the tissue section for subsequent incubations, as well as a protective layer to minimize the interaction between the charged glass surface with the reagents.

BIOBOND is suitable for all kinds of tissue specimens including but not limited to paraffin wax, resin sections, cell smears, cytospins and cryostat sections.

BIOBOND is supplied in a 20ml vial, which is sufficient to coat at least 1000 slides.

0-5ºC

71304

Biobond

20 ml

50.00

BioMeda™Substitutes

Fluoro-Gel - a direct substitute for BioMeda™ Gel-Mount
Clear-Mount - a direct substitute for BioMeda™ Crystal Mount
In-Situ Mount - a direct substitute for BioMeda™ Situ Mount
Limonene-Mount  - a direct substitute for BioMeda™ Clarion Mount

Fluoro-Gel (A Direct Substitute for BioMeda™ Gel-Mount)

Fluoro-Gel is a water based mounting medium designed for the permanent mounting of fluorescent stained tissues, which may either be damaged or soluble in organic solvents, such as xylene or toluene. This unique formula prevents rapid photo-bleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa Fluoro 488, Alexa Fluoro 594, Green Fluorescent Protein (GFP), Tetramethylyrhodamine, Redox, Phycoerythrin (RP-E), Phyocyanin (PC), and Allophycocyanin (APC). The fluorescence is retained during prolonged storage at 4ºC in the dark.

Application: Immunofluorescence, confocal microscope.

For our original formula:

0-5ºC	17985-10	Fluoro-Gel, (with Tris Buffer)	20 ml	35.00
0-5ºC	17985-11	Fluoro-Gel, (with Tris Buffer)	100 ml	128.00

For a more stable pH:

0-5ºC	17985-30	Fluoro-Gel, (with TES Buffer)	20 ml	35.00
0-5ºC	17985-31	Fluoro-Gel, (with TES Buffer)	100 ml	128.00
0-5ºC	17985-40	Fluoro-Gel, (with PIPE Buffer)	20 ml	35.00
0-5ºC	17985-41	Fluoro-Gel, (with PIPE Buffer)	100 ml	128.00

*The names BioMeda™ Gel Mount and Crystal Mount are all owned by BioMeda Corporation

Fluoro-Gel II Mounting Medium

Fluoro-Gel II is similar to Fluoro-Gel, but Fluoro-Gel II contains DAPI (4, 6-diamino-2-phenylindole), which is a counter stain for DNA. This product is to be used in in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360 nm and emits at 460 nm, producing blue fluorescence. RNA is also stained with DAPI.

Application: Immunofluorescence, confocal microscopy.

0-5ºC	17985-50	Fluoro-Gel II	20 ml	59.00
0-5ºC	17985-51	Fluoro-Gel II	100 ml	210.00

Fluoro-Gel III Mounting Medium

Fluoro-Gel III Mounting Medium is similar to Fluoro-Gel, however, Fluoro-Gel III with the addition of PI (Propidium Iodide) which is a counterstain for DNA, makes this a unique mounting medium, which prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa Fluoro 488, Alexa Fluoro 594, Green Fluorescent protein (GFP), Tetramethylyrhodamine, Redox, Phycoerythrin (RP-E), Phyocyanin (PC), and Allophycocyanin (APC). The fluorescence is retained during prolonged storage at 4ºC in the dark. This medium does not contain Phynylenediamine, which destroys immunofluoresccence of Cy dyes, RP-E, PC, and APC. This product is to be used in in situ hybridization techniques or other method where fluorescence of DNA staining is required. PI excites at 535 nm, emits at 615 nm, producing a red fluorescence. RNA is also stained with PI.

Application: Immunofluorescence, confocal microscope

0-5ºC	17985-60	Fluoro-Gel III	20 ml	59.00
0-5ºC	17985-61	Fluoro-Gel III	100 ml	210.00

Clear-Mount  (A Direct Substitute for BioMeda™ Crystal Mount)

Clear-Mount is a water based mounting medium designed for the permanent mounting of hydrated tissues, which may be damaged with organic solvents.  Such samples include cell smears, with peroxidase and alkaline phosphatase chromogens.

This mounting medium preserves Fast Red, Aminoethylycarbazole (AEC), NBT/BCIP, INT/BCIP chromogens and is also compatible with counter-stain such as Hematoxylin and Nuclear Fast Red. It is also suitable for chromogens like DAB and DAB with Nickel and Cobalt. It is not compatible with H & E staining.

Application: Mounting of Immunohisto slides.

For our original formula:

0-5ºC	17985-12	Clear-Mount, (with Tris Buffer)	30 ml	52.00
0-5ºC	17985-15	Clear-Mount, (with Tris Buffer)	250 ml	280.00

For a more stable pH:

0-5ºC	17985-16	Clear-Mount, (with PIPE Buffer)	30 ml	52.00
0-5ºC	17985-17	Clear-Mount, (with PIPE Buffer)	100 ml	125.00

*The names BioMeda™ Gel Mount and Crystal Mount are all owned by BioMeda Corporation

In-Situ Mount (A Direct Substitute for BioMeda™ Situ Mount)

In-Situ mounting medium is a unique mounting medium designed for permanent preservation of tissue sections and cell smear with alkaline phosphatase chromogens like NBT/BCIP and red stain.

Application: Mounting tissues and cell smears.

0-5ºC

17988-30

In-Situ Mounting Medium

30 ml

54.00

*The name BioMeda™ Situ Mount is owned by BioMeda Corporation

Limonene-Mount  (A Direct Substitutefor BioMeda™ Clarion Mount)

This mounting medium is made with limonene, a natural product from orange peels. It preserves  tissues and cell smears that can be dehydrated with organic solvents in immunohistochemistry, e.g. DAB and DAB with nickel or cobalt. Our Limonene-Mount also works well with alkaline phosphatase chromogens, and organic solvent resistant Supper Fast Red. It is also a good choice for mounting H & E stained slides.

Application: Mounting tissues and cell smears.

0-5ºC	17987-01	Limonene-Mount	100 ml	178.00
0-5ºC	17987-06	Limonene-Mount	6x100 ml	960.00
0-5ºC	17987-25	Limonene-Mount	250 ml	210.00

*The name BioMeda™ Clarion is owned by BioMeda Corporation.

Buffers

Universal Immunoassay Buffer™, 10X
Universal Antibody Dilution Buffer™
Antibody Dilution Buffer with Immunoglobulin- Free BSA

Biomount™

Some mounting media oxidize rapidly upon exposure to air, forming carboxyl groups. This can be more pronounced when sections have been cleared in a solution containing aldehyde. It is sometimes observed that the immunogold/silver signal fades after a few weeks, or even in a shorter time, from sections that have been mounted with these media under cover slips. The silver is still present, but has formed translucent silver carboxylate salts. Visibility can be retrieved by removing the cover slip and washing in xylene and then immersing the slide in photographic developer, but this is a tedious procedure.
BIOMOUNT is a specially formulated mounting medium that reduces fading of immunogold/silver signals in sections on glass slides. It is suitable for resins as well as wax embedded sections of tissue.

RT

17894

BioMount

100ml

40.00

Certified Conductive Adhesives:

EMS introduces a new reliable line of conductive adhesives which are certified by the Bureau Veritas Quality International. Quality Standards: ISO 9001; EN29001; ANSI/ASQC Q91

Silver Conductive 18DB70X

Silver Conductive Coating 18DB70X is a direct substitute for Silver Conductive Fluid 416, which is no longer available. This material was specially formulated for use in geographic areas that demand the use of low VOC (Volatile Organic Compounds) products. It also provides low ohms at very thin dry film thickness on almost any surface, and exhibits excellent long term shielding and grounding properties.

Typical Properties:

As supplied (Liquid)

Pigment	:	Silver
Binder	:	Acrylic
Solids content by weight:	:	50.8% ± 5%
Density:	:	13.9 lb/gal (1.67kg/l)
	:

As applied:

VOC	:	0.5 lb/gal (59.6g/l)
Diluent:	:	Acetone (1:1 ratio by volume)
Drying time:	:	5 minutes air dry to touch/10 minutes to handle then 5 minutes at 180°–225°F(82° – 107°C) or air cure for 24 hrs.

When dried:

Sheet resistance:	:	0.015 ohms/sq. in./mil (25µm)
Attenuation:	:	75 dB

RT

12684-15

Silver Conductive Coating

15 g

22.00

Silver Conductive Adhesive Fluid 504

Replaced by Silver Conductive Adhesive 503.

Silver Conductive Adhesive Paste 478SS

Our adhesive 478SS is a conductive, silver-based polymer which is used for thick film coatings where liquid silver is not an option. Once cured, it offers a very high Tg (glass transition temperature) 153°F (67°C) to prevent blocking, and offers superior adhesion to polyester film. Adhesive 478SS can be cured at 200°F (93°C) within 15 minutes. Higher temperatures will reduce the time needed to achieve a final cure.

Typical Properties:

* As Supplied

Pigment	:	Silver
Binder	:	Polyester
Color	:	Silver
Dilutent	:	Carbitol acetate
Consistency	:	Paste (13,000-28,000 cps)
Solid content	:	72.5-75.5%
Flash point	:	230°F (110°C)
Shelf life	:	6 months under original seal

* As Cured

Color	:	Silver
Sheet resistance	:	<0.025 ohm/sq @1 mil

RT	12685-15	Silver Conductive Adhesive 478SS	15g	45.00
RT	12685-25	Silver Conductive Adhesive 478SS Thinner	25ml	8.00

Silver Conductive Adhesive 503

A High Temperature Conductive Paint

Silver paint 503 is a flexible, high temperature conductive material designed for a wide variety of uses, and adheres to most substrates.

ADVANTAGES:

• Withstands ambient temperatures of over 500°F (260°C)
• Remains flexible over temperature range of –40°F to 500°F
• Highly conductive – good adhesion to substrates
• Dries at room temperature
• Ready to use – easy to apply

Typical Properties (as supplied):

Pigment	:	Specially treated silver 56%
Binder	:	Fluoroelastomer
Carrier	:	Methyl Isobuthyl Ketone (MIBK)
Color	:	Silver - Consistency: fluid
Density	:	14.6 lbs/gal (1.75kg/L)
Solid content by weight	:	18%
Weight solids	:	62%
Viscosity	:	1700cps

Shelf life for this product is two years under original seal. Store in cool place.

Typical Properties (as Cured):

Color	:	Silver
Service temperature	:	525°F (275°C)
Sheet resistance	:	0.05 ohms/sq. in/ 1mil dry film

Drying

Air dry coated part approximately 10 minutes (depending on humidity) before carrying out resistance hecks. Air dry to touch in 30 seconds and it is ready for use in 2 minutes.

12686-15

Silver Conductive Adhesive 503

15g

27.00

Graphite Conductive Adhesive 154

Adhesive 154 is an easy-to-apply resistance coating designed to provide high lubricity, conductivity, and excellent release properties to many non-conductive substrates, including most plastics. It is made from a dispersion of colloidal graphite in an isopropanol base which quickly air-dries, forms an uniform thin film adherent layer. Air dries to touch in 5 minutes and is ready for use in 30 minutes. After air drying, bake for 5 minutes at 167°F (75°C) to achieve optimum coating qualities in a shorter curing cycle.

Specifications:

* As supplied

Pigment	:	Graphite
Color	:	Black
Binder	:	Celluosic resin
Carrier	:	Isopropanol
Dilutent	:	Isopropanol or equivalent
Consistency	:	Liquid
Weight solids	:	20%
Volume solids	:	14%
Flash point	:	52°F (11°C)
Shelf life	:	6 months under original seal

* As cured

Color	:	Matte black
Service temp	:	150°F (65°C)
Sheet resistance	:	1.2 K ohms/sq inch @1 mil dry film

RT

12691-30

Graphite Conductive Adhesive 154

30g

9.00

Graphite Conductive Adhesive 112

Adhesive 112 is an air drying graphite coating of unusually high conductivity. It provides excellent static bleed properties and acts as a protective energy absorbing layer. It also offers good shielding performance (30-50 db over 50-450 MHz) at a coating thickness of 2 mils. It is water based and useful in solvent prohibited applications.

To use: Air dry until all water has flashed off. Air dries to touch in 20 minutes, to handle in 25 minutes. It will continue to harden for 24 hours. It can be forced dried at temperatures up to 160°F (71°C).

Specifications:

* As supplied

Pigment	:	Graphite
Color	:	Black
Binder	:	Acrylic
Volume solids	:	34%
Flash point	:	52°F (11°C)
Shelf life	:	6 months under original seal

* As cured

Color	:	Black
Service temp	:	350°F (117°C)
Sheet resistance	:	20 ohms/square @ 1 mil

RT

12693-30

Graphite Conductive Adhesive 112

30g

9.00

Carbon Conductive Adhesive 502

A High Temperature Conductive Paint

Conductive adhesive 502 is a combination of specially processed carbon particles in a fluoroelastomer resin system designed to provide high resistance values. In its cured form, it exhibits both high and low temperature flexibility and moisture resistance.

Advantages:

• Withstands ambient temperatures of over 500°F (260°C)
• Remains flexible over temperature range of –40°F to over 500°F (260°C)
• Cures at room temperature
• Good adhesion to a variety of substrate
• Excellent oxidation resistance
• Ready to use - easy to apply

Typical Properties (as supplied)

Pigment	:	Specially Processed Carbon
Binder	:	Fluoroelastomer
Diluent	:	MethylEethylKketone (MEK)
Color	:	Black
Consistency	:	Fluid
Density	:	7.2lbs/gal (0.87 kg/l)
Solid content by weight	:	13%
Viscosity	:	600 ± 200 mPas (Brookfield RVT @ 20rpm)
Flask point	:	23°F (-°5C)

Typical Properties (as cured)

Color	:	Black
Max service Temperature	:	525°F (275°C)
Sheet resistance	:	130 ± 100 ohms/sq.in./ 1 mil dry film

Shelf life for this product is one year under original seal. Store in cool place

Drying

Air drying of the product is adequate for most applications. To assure complete solvent loss, the coating can be baked for 15 minutes at 302°F (150°C).

RT

12694-30

Carbon Conductive Paint 502

30 g

16.00

Colloidal Compunds and Conductive Adhesives

A) Silver Adhesives:

* Liquid; Colloidal Silver: Acetone base. Fast drying. Average grain size less than 10µm. Comes with a brush attached to the cap. Service temperature is 30 minutes at 200°C.

RT	12630	Silver Liquid	15g	26.50
RT	12641	Silver Colloidal Extender	25ml	3.95

* Paste; Colloidal Silver: Thick base. Easily applied with a wooden pick*.

RT	12640	Silver Paste	25g	43.00
RT	12641	Silver Colloidal Extender	25ml	3.95

B) Graphite Adhesives:

-Water Base: Flat surface texture. The average flake size is 1µm.
Service temperature: 306°C.

RT

12650

Graphite, Water base

50g

8.50

-Isopropanol base: Flat surface texture. The average flake size is 1µm.
Service temperature is 204°C.

RT	12660	Graphite, Isopropanol base	30g	7.65
RT	12661	Graphite Extender	30ml	6.50

C) CCC Carbon Adhesive

An electrically Carbon Conductive Cement for specimen mounting in all SEM work. After drying of the cement, immediate investigation of conductive specimens is possible. Non-conductive specimens need only to be coated with carbon or metal.

Thinner is available if the cement viscosity is too thick.

RT	12664	CCC Adhesive	30g	37.50
RT	12665	CCC Thinner	30ml	10.00

D) Leit-C-Plast

A special adhesive with very high electrical conductivity and permanent plasticity for the preparation of big specimens in SEM work.

RT

12667

Leit-C-Plast

15g

55.00

E) Tempfix Adhesive

A thermoplastic adhesive for mounting powder specimens and small particles for SEM. It does not contain any solvents and it is stable in high vacuum. It is not sticky at room temperature but becomes adhesive at 40°C and melts at 120°C. Tempfix may also be used as an embedding medium.

RT

12668

Tempfix Adhesive Set

each

38.00

Technical Tip:

Mounting of Powders, Granules, and Fibers

CITIFLUOR™ - Antifadent Mountant Solutions

A mounting medium for immunofluorescence, which overcomes the problems of fading.

This mounting media is designed to reduce the fading of the fluorescence of dyes used for labeling biological specimens.

In the technique of fluorescence microscopy, the fluorescence is stimulated by high intensity UV and/or visible light. Absorption of the light populates an excited state of the dye (usually a singlet state) and this leads to fluorescence. However, the excited state of the dye may undergo chemical reaction, which leads to destruction as evidenced by the fading of the fluorescence and consequent loss of the image. Citifluor™ mounting media is designed to solve this problem.

Selection of the appropriate product:

There is no universal antifadent due to the fact that biological material is so varied in nature that the fluorescence fading of fluorochromic conjugates is a complex process.

The general anti-fadents are AF1, AF2, and AF3. The AF87 is a specialist immersion oil-based antifadent.

AF1 (Glycerol-phosphate buffered solution containing an additive for use with labeled tissue sections). Should be used if you normally examine specimens in glycol solution. If you normally use saline solution with your specimens.

AF2 (Glycerol solution containing additives for use with labeled tissue sections) is simply a glycerol solution of the antifadent.

AF3 (Phosphate-buffered saline solution containing additives for examination of whole cell) is appropriate.

AF87, the antifadent, is contained in non-fluorescent immersion oil. It is designed for those requiring work at high magnification. It is useful for preventing the fading of such dyes as DAPI, which have the tendency to disperse in glycerol based mountant solutions.

CFPVOH is an aqueous solution of poly(vinyl alcohol) for use as a solid CFPVOH is an aqueous solution of poly (vinyl alcohol) for use as a solid mountant. Apply a few drops of the solution to your sample, and allow drying to give a clear film.

AF100 is a solution of antifadents to be used with CFPVOH when fading of fluorescence in the poly(vinyl alcohol) is a problem. To prepare a CFPVOH solution containing AF100, add 0.1ml of Af100 to 0.9ml of CFPVOH. If more or less of the solid mountant solution is required, mix
the reagent in the appropriate ratio. Once these solutions have been made up, they should be used within a period of days since the solution deteriorates on storage.

All of these products have a long shelf life, a minimum of 2 years. The solution can be stored at room temp-erature. With LD50’s >2000mg/kg (in rat), this product exhibits no toxicological problems.

RT	17970-25	CitiFluor™ AF1	25 ml	86.00
RT	17970-100	CitiFluor™ AF1	100 ml	190.00
RT	17971-25	CitiFluor™ AF2	25 ml	86.00
RT	17971-100	CitiFluor™ AF2	100 ml	190.00
RT	17972-25	CitiFluor™ AF3	25 ml	86.00
RT	17972-100	CitiFluor™ AF3	100 ml	190.00
RT	17976-10	CitiFluor™ AF87	10 ml	64.00
RT	17976-25	CitiFluor™ AF87	25 ml	115.00
RT	17978-35	Set of CitiFluor™ CFPVOH and AF100*	25 ml + 5 ml	96.00

* Only available when ordering with CFPVOH

• Citifluor AF1, AF2 & AF3
• Citifluor AF87
• Citifluor CFPVOH AND AF10

Mounting Medium for all Laser Scanning and Conventional Microscopes

A Revolutionary New Mounting Medium which helps to control the effects of Refraction.

THESE MOUNTANT SOLUTIONS:

• Have a refractive index of 1.52 to match the refractive index of your slides and coverslips
• Are simple to use – Just apply the solution to the specimen followed by a cover slip
• The solution may be kept at room temperature – comes with dropper tip to aid in dispensing
• All mountants are water miscible (aqueous glycerol based), and therefore the specimen does not need to be dehydrated prior to the addition of the mounting solution.
• Has a pH compatible with the specimens: CFM-1 has a pH of ~6.5, and CFM-2 has a pH of ~8.5
• Contains an antifadent to help reduce the bleaching of the fluorochromes (CFM-1 Plus Antifadent)
• May be used as a immersion oils in addition to being a mountant solution and since they are water-based, they facilitate cleaning the microscope optics.

CFM-1 and CFM-1 Plus Antifadent

This glycerol-phosphate buffered saline based mountant solution has been specially formulated so as to have a refractive index of ~1.52 @ room temperature. The addition of antifadent is to retard the bleaching of fluorochromes. Its refractive index is similar to glass; therefore effects