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Electron Microscopy Sciences

Chemicals for Microscopy

arrow Ready To Use Prepared Buffers

Ready To Use Prepared Buffers All of our ready-to-use buffers are:

  • Conveniently packaged.
  • Prepared fresh for each order.
  • Economical and time-saving.
  • Desired pH should be specified when ordering.

The buffer is a vehicle for the fixative and requires certain properties:

  1. To maintain constant pH during the time of the fixation. The pH of the fixative in EM is adjusted using the buffer system chosen; e.g. range 6.5-8.0.
  2. The ionic constitution to prevent the extraction of cellular components, or the deposition of precipitates of fixative chemicals.

msdsarrowAcetate Buffer

Acetic Acid and Sodium Acetate
0.2M
pH range 3.6-5.6

0-5°C 11480 Acetate Buffer 225 ml 13.00 Add to cart
0-5°C 11482 Acetate Buffer 500 ml 18.00 Add to cart

arrowAcetate Buffer

RT 11482-42 Acetate Buffer, 0.2M, pH 4.2 500 ml 18.00 Add to Cart
RT 11482-50 Acetate Buffer, 0.2M, pH 5.0 500 ml 18.00 Add to Cart
RT 11482-56 Acetate Buffer, 0.2M, pH 5.6 500 ml 18.00 Add to Cart

arrowAntibody Dilution Buffer with Immunoglobulin- Free BSA

This ready-to-use Antibody Dilution Buffer is designed for the dilution of antibodies in all immunoassays, including immunofluorescence, IHC, ELISA, WB. This buffer contains immunoglobulin free BSA and does not contain phosphate, sodium azide, or mercury preservatives. It can be used for the dilution of all antibodies, including peroxidase, and antibodies to phophoproteins. However, it is not suitable for dilution of antibodies to S100 proteins. This buffer contains green food color.

Reagent Supplied: Ready to use buffer

Application: Dilute antibodies, primary or secondary as recommended in the protocol by the manufacturer of these products.

Storage: 2 – 8°C. Diluted antibodies can be stored at 2 – 8°C for several weeks, depending upon the stability of antibodies.

2–8°C 25886-01 Antibody Dilution Buffer, free BSA 100 ml 50.00 Add to Cart
2–8°C 25886-05  Antibody Dilution Buffer, free BSA    500 ml  100.00 Add to Cart
  1227SK Antigen Preserve Solution VII 1L 150.00 Add to Cart

arrow12Borate Buffer

RT 11455-78 Borate Buffer, pH 7.8 30.00 500 ml Add to Cart
RT 11455-82 Borate Buffer, pH 8.2 30.00 500 ml Add to Cart
RT 11455-90 Borate Buffer, pH 9.0 30.00 500 ml Add to Cart

msdsarrowCitrate Buffer

Citric acid and Sodium citrate 0.1M
pH range 4.6-6.2

0-5°C 11490 Citrate Buffer 0.1M 225 ml 13.00 Add to Cart
0-5°C 11492 Citrate Buffer 0.1M 450 ml 18.00 Add to Cart

arrowCitrate Buffer

Citric acid and Sodium citrate 0.2M

0-5°C 11492-04 Citrate Buffer 0.2M, pH 4.0 1L 37.00 Add to Cart
0-5°C 11492-06 Citrate Buffer 0.2M, pH 6.0 1L 37.00 Add to Cart

msdsarrow12Dulbecco’s Phosphate Buffer Saline – D-PBS

All of our Dullbecco’s Phosphate Buffer Saline Solutions contain: Potassium Chloride, Potassium Phosphate Monobasic, Sodium Chloride, Sodium Phosphate Dibasic, and D-Glucose. Some formulations may contain additional components. Use the table below as a quick reference for identifying the one that best suits your application. All of the solutions are prepared 1X strength.

Ingredients F.W. Formula I Formula II Formula III
KCl 74.56 X X X
NaCl 58.44 X X X
Na2HPO4·7H2O 268.07 X X X
KH2PO4 136.09 X X X
CaCl2·2H2O 147.02 X X X
MgCl2·6H2O 203.31 X X X
Phenol Red   ---- ---- 0.2g/L
RT 11772-05 D-PBS, Formula I, 0.15M 500 ml 20.00 Add to Cart
RT 11772-10 D-PBS, Formula I, 0.15M 1 L 25.00 Add to Cart
RT 11773-05 D-PBS, Formula II, 0.19M 500 ml 20.00 Add to Cart
RT 11773-10 D-PBS, Formula II, 0.19M 1 L 25.00 Add to Cart
RT 11774-05 D-PBS, Formula III, 0.15M 500 ml 20.00 Add to Cart
RT 11774-10 D-PBS, Formula III, 0.15M 1 L 25.00 Add to Cart

arrow12EARLE's Balanced Salt Solutions - EBSS

All of our Earle’s Balanced Salt Solution contains: Potassium Chloride, Sodium Chloride, Sodium Phosphate Monobasic, and D-glucose. Some formulations may contain additional components. Use the table below as a quick reference for identifying the one that best suits your application. All of the solutions are prepared 1X strength.

Ingredients F.W. Formula I Formula II Formula III
KCl 74.56 X X X
NaCl 58.44 X X X
NaH2PO4·H2O 137.99 X X X
D-Glusose 180-16 1g/L X 1g/L
MgSO4·7H2O 246.48 X X X
CaCl2,·2H2O 147.02 X X X
NaHCO3 84.01 X X X
Phenol Red   ---- 0.015g/L 0.050g/L
RT 11765-05 EBSS, Formula I, 0.157M 500 ml 16.00 Add to Cart
RT 11765-10 EBSS, Formula I, 0.157M 1 L 21.00 Add to Cart
RT 11766-05 EBSS, Formula II, 0.152M 500 ml 16.00 Add to Cart
RT 11766-10 EBSS, Formula II, 0.152M 1 L 21.00 Add to Cart
RT 11767-05 EBSS, Formula III, 0.157M 500 ml 16.00 Add to Cart
RT 11767-10 EBSS, Formula III, 0.157M 1 L 21.00 Add to Cart

arrowHepes, Good's Buffer Crystalline

Please see listing in Chemicals for Microscopy.

msdsarrowHepes Buffer Solution

0.2M
pH range: 7.0-8.0

0-5°C 11494 Hepes Buffer Solution 500 ml 18.00 Add to Cart

arrow12Michel’s Buffer

RT 18041-05 Michel’s Buffer 500 ml 19.00 Add to Cart
RT 18041-10 Michel’s Buffer 1 L 25.00 Add to Cart

msdsarrowMillonig's Phosphate Buffer

Millonig (1964); Karlsson and Schultz (1965)
Ingredients:
Sodium Phosphate, monobasic (NaH2PO4xH2O)
Sodium Phosphate, dibasic (Na2HPO4xH2O)
0.5% Sodium Chloride (NaCl)
Shipping Conditions: Ambient Temperature
Maximum shelf life after receipt: 1 month

The pH of this buffer is 7.4 (0.1M); the osmolarity is 440 mosmols, so this buffer is hypertonic to most body fluids. Millonig recommended to use this buffer for the fixation of very hydrated tissues with osmium tetroxide. For marine organisms, a higher concentration of sodium chloride (3%) is recommended. We supply it in a double strength (0.2M), pH 7.4. Dilution is needed before use.

0-5°C 11582-05 Millonig's Phosphate Buffer 0.2M 500 ml 18.00 Add to Cart
0-5°C 11582-10 Millonig's Phosphate Buffer 0.2M 1 L 27.00 Add to Cart

msdsarrowMops, Biological Buffer

(3-(N-Morpholino) propanesulfonic acid)
pH range 6.5-7.9

0-5°C 18900 Mops, Biological Buffer 100g 44.00 Add to Cart

arrowPhosphate Buffer, 0.1M*

Prepared from Sodium Phosphate Monobasic Hydrate and Sodium Phosphate Dibasic Heptahydrate. Non-Sterile. Meant for research purposes only.

0-5°C 19340-60 Phosphate Buffer, pH 6.0 1 L 35.00 Add to Cart
0-5°C 19340-65 Phosphate Buffer, pH 6.5 1 L 35.00 Add to Cart
0-5°C 19340-70 Phosphate Buffer, pH 7.0 1 L 35.00 Add to Cart
0-5°C 19340-72 Phosphate Buffer, pH 7.2 1 L 35.00 Add to Cart

*Meant for biological research only. Not to be used for medical or any other purposes.

arrowPhosphate Buffer Saline (PBS)

PBS is commonly used in biochemistry. It is a salty solution containing calcium chloride, sodium phosphate and potassium phosphate. PBS is isotonic and non-toxic to cells. 1X PBS final concentration is 0.137M NaCl, 0.01M Na2HPO4, 0.0027M KCl and pH 7.4.

RT 19342-10 Phosphate Buffered Saline, 10X 1L 26.00 Add to Cart
RT 19242-40 Phosphate Buffered Saline, 10X 1 gal 95.00 Add to Cart
RT 19343-10 Phosphate Buffered Saline, 2X 1L 23.00 Add to Cart
RT 19343-40 Phosphate Buffered Saline, 2X 1 gal 82.00 Add to Cart
RT 19344-10 Phosphate Buffered Saline, 1X 1L 19.00 Add to Cart
RT 19344-20 Phosphate Buffered Saline, 1X 1 gal 70.00 Add to Cart

arrowPipes, Good's Buffer Crystalline

Please see listing in Chemicals for Microscopy.

Araldite 502arrowPipes Buffer Solution, 0.3M

Aqueous PIPES solution adjusted by 0.1N Sodium Hydroxide to pH 5.5-6.0.

0-5°C 11610 Pipes Buffer Solution, 0.3M 450 ml 25.00 Add to Cart

msdsarrowSodium Cacodylate Buffer

Prepared from Sodium Cacodylate Trihydrate (F.W. 214.02)

0-5°C 11650 Sodium Cacodylate Buffer 225 ml 23.00 Add to Cart
0-5°C 11652 Sodium Cacodylate Buffer 450 ml 35.00 Add to Cart
0-5°C 11653 Sodium Cacodylate Buffer, 0.2M, pH 7.4 1 L 76.00 Add to Cart
0-5°C 11654 Sodium Cacodylate Buffer, 0.4M, pH 7.2 500 ml 52.00 Add to Cart
0-5°C 11655 Sodium Cacodylate Buffer, 0.4M, pH 7.2 1 L 99.00 Add to Cart

msdsarrowSorensen's Phosphate Buffer

(Phosphate Mixed Sodium Salts)
Sorensen, see Dawson et. al, (1969)
Sodium Phosphate, dibasic (Na2HPO4xH2O)
Sodium Phosphate, monobasic (NaH2PO4xH2O)
0.2M, pH range 5.8 - 8.0
Shipping Conditions: Ambient Temperature
Maximum shelf life after receipt: 3 months

The osmolarity of a 0.1 M at a pH 7.2 is 226 mosmols; with the addition of 0.18 M of sucrose to the solution, the osmolarity raises to 425 mosmols.
Standard pH 7.2; 0.2M is supplied. Otherwise, specify pH when ordering.

0-5°C 11600-05 Sorensen's Phosphate Buffer, pH 7.2 500 ml 18.00 Add to Cart
0-5°C 11600-10 Sorensen's Phosphate Buffer, pH 7.2 1 L 27.00 Add to Cart
0-5°C 11600-10 Sorensens Phosphate Buffer, 0.2M, pH 7.2 1 L 27.00 Add to Cart
0-5°C 11600-40 Sorensens Phosphate Buffer, 0.2M, pH 7.2 4 L 99.00 Add to Cart
0-5°C 11601-10 Sorensens Phosphate Buffer, 0.2M, pH 7.4 1 L 27.00 Add to Cart
0-5°C 11601-40 Sorensens Phosphate Buffer, 0.2M, pH 7.4 4 L 99.00 Add to Cart
0-5°C 11682-05 Sorensen's Phosphate Buffer, 0.15M 500 ml 19.00 Add to Cart
0-5°C 11682-10 Sorensen's Phosphate Buffer, 0.15M 1 L 27.00 Add to Cart

msdsAraldite 502arrowSym-Collidine Buffer Kit

2,4,6-Trimethylpyridine
N:C(CH3)CH:C(CH3)CH:CCH3   CAS #108-75-8   F.W. 121.18
Bennett & Luft., Biophys & Biochem. Cytol., 6,113 (1959).

A very stable buffer, used with Osmium tetroxide; provides excellent fixation, better stability and buffering capacity. The pH can be adjusted by varying the amount of hydrochloric acid in the final volume of 200 ml. Preparation Instructions: Pour 9ml of 2N HCl; add 5.34ml of Sym-Collidine, mix well and add distilled water up to 200 ml for a pH 7.4 solution.

Set consists of:

  • 1 amp 5.34ml Sym Collidine
  • 1 amp 9.0 ml of 2N HCl
0-5°C 11500 Sym-Collidine Buffer Kit Kit 25.00 Add to Cart
0-5°C 11510 Sym-Collidine Buffer 5 kit/bx 100.00 Add to Cart

arrowSym-Collidine, Reagent

2,4,6-Collidine; 2,4,6-Trimethylpyridine
(C5H2N)(CH3)3   CAS #108-75-8   F.W. 121.18
Assay: 99% Specific Gravity: 0.9170

RT 11520 Sym-Collidine, Reagent 100 ml 110.00 Add to Cart

arrowTris, (hydroxymethyl) aminomethane, Crystalline

Please see listing in Chemicals for Microscopy.

msdsarrowTris, (hydroxymethyl) aminomethane maleate

Tris Maleate Buffer

0.2M pH range 6.4-8.0

0-5°C 11730 Tris Maleate Buffer 250 ml 17.00 Add to Cart
0-5°C 11740 Tris Maleate Buffer 500 ml 25.00 Add to Cart
Tris Buffer

0.2M pH 8

RT 11730-05 Tris Buffer 0.2M pH 8.0 250 ml 17.00 Add to Cart
RT 11730-06 Tris Buffer 0.2M pH 8.0 500 ml 25.00 Add to Cart
Tris Buffered Saline

pH 7.4

RT 11730-08 Tris Buffered Saline pH 7.4 250 ml 17.00 Add to Cart
RT 11730-09 Tris Buffered Saline pH 7.4 500 ml 25.00 Add to Cart
Tris-HCL Buffer

1M pH 6-8

RT 11730-12 Tris-HCL Buffer 1M pH range 6-8 250 ml 17.00 Add to Cart
RT 11730-15 Tris-HCL Buffer 1M pH range 6-8 500 ml 25.00 Add to Cart
Tris HCL Buffer

pH range 7.2 - 9.0

RT 11730-20 Tris HCL Buffer pH range 7.2-9.0 250 ml 17.00 Add to Cart
RT 11730-25 Tris HCL Buffer pH range 7.2-9.0 500 ml 25.00 Add to Cart

technical data sheet arrowUniversal Immunoassay Buffer™, 10X

Immunoassay buffer is used as a 1X  in all immunoassays (immunofluorescence, IHC, ELISA, WB etc.) as a washing buffer  and it can be used as a dilution buffer for antibodies; however it does not contain any carrier proteins.

This buffer also does not contain any phosphate, sodium azide or mercury as preservatives and can be used for dilution of all antibodies, including peroxidase, antibodies to phosphoproteins. It is  not suitable for dilution of antibodies to S100 proteins.

Reagent Supplied: 10X Buffer

Application: Dilute this 10X buffer 10 times with DI water. (90 ml water + 10 ml this buffer – mixed well). Wash 3-5 times with this buffer in between the steps of your immunoassay protocol.

Storage: Room temperature

RT 25880-01 Universal Immunoassay Buffer, 10X   100 ml 50.00 Add to Cart
RT 25880-10 Universal Immunoassay Buffer, 10X  1 L 100.00 Add to Cart

technical data sheet arrowUniversal Antibody Dilution Buffer™

This ready-to-use Universal Antibody Dilution Buffer is designed for dilution of antibodies in all immunoassays, including immunofluorescence, IHC, ELISA, WB. This buffer does not contain any mammalian proteins, phosphate, sodium azide, or mercury preservatives and can be used for dilution of all antibodies, including peroxidase, and antibodies to phophoproteins. However, it is not suitable for dilution of antibodies to S100 proteins.

Reagent Supplied: Ready to use buffer

Application: Dilute antibodies, primary or secondary as recommended in the protocol by the manufacturer of these products.

Storage: 2–8°C. Diluted antibodies can be stored at 2–8°C for several weeks, depending upon the stability of antibodies.

2–8°C 25885-01 Universal Antibody Dilution Buffer  100 ml 50.00 Add to Cart
2–8°C 25885-05  Universal Antibody Dilution Buffer 500 ml 100.00 Add to Cart

Cacodylic to Durcupan arrow14

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© 2011 Electron Microscopy Sciences

Electron Microscopy Sciences specializes in the manufacturing, preparation and distribution of the highest quality laboratory chemicals and microscopy supplies and equipment for electron microscopy, light microscopy, and histology.