Acetate Buffer

Acetic Acid and Sodium Acetate
0.2M
pH range 3.6-5.6

0-5°C	11480	Acetate Buffer	225ml	13.00	Add to cart
0-5°C	11482	Acetate Buffer	500ml	18.00	Add to cart

Antibody Dilution Buffer with Immunoglobulin- Free BSA

This ready-to-use Antibody Dilution Buffer is designed for the dilution of antibodies in all immunoassays, including immunofluorescence, IHC, ELISA, WB. This buffer contains immunoglobulin free BSA and does not contain phosphate, sodium azide, or mercury preservatives. It can be used for the dilution of all antibodies, including peroxidase, and antibodies to phophoproteins. However, it is not suitable for dilution of antibodies to S100 proteins. This buffer contains green food color.

Reagent Supplied: Ready to use buffer

Application: Dilute antibodies, primary or secondary as recommended in the protocol by the manufacturer of these products.

Storage: 2 – 8°C. Diluted antibodies can be stored at 2 – 8°C for several weeks, depending upon the stability of antibodies.

2–8°C	25886-01	Antibody Dilution Buffer, free BSA	100 ml	40.00
2–8°C	25886-05	Antibody Dilution Buffer, free BSA	500 ml	99.00

Citrate Buffer

Citric acid and Sodium citrate
0.1M
pH range 4.6-6.2

0-5°C	11490	Citrate Buffer	225ml	13.00
0-5°C	11492	Citrate Buffer	450ml	18.00

Hepes, Good's Buffer Crystalline

Please see listing in Chemicals for Microscopy.

Hepes Buffer Solution

0.2M
pH range: 7.0-8.0

0-5°C

11494

Hepes Buffer Solution

500ml

18.00

Millonig's Phosphate Buffer

Millonig (1964); Karlsson and Schultz (1965)
Ingredients:
Sodium Phosphate, monobasic (NaH₂PO₄xH₂O)
Sodium Phosphate, dibasic (Na₂HPO₄xH₂O)
0.5% Sodium Chloride (NaCl)
Shipping Conditions: Ambient Temperature
Maximum shelf life after receipt: 1 month
The pH of this buffer is 7.4 (0.1M); the osmolarity is 440 mosmols, so this buffer is hypertonic to most body fluids. Millonig recommended to use this buffer for the fixation of very hydrated tissues with osmium tetroxide. For marine organisms, a higher concentration of sodium chloride (3%) is recommended. We supply it in a double strength (0.2M), pH 7.4. Dilution is needed before use.

0-5°C	11582-05	Millonig's Phosphate Buffer 0.2M	500ml	17.00
0-5°C	11582-10	Millonig's Phosphate Buffer 0.2M	1 L	26.00

Mops, Biological Buffer

(3-(N-Morpholino) propanesulfonic acid)
pH range 6.5-7.9

0-5°C

18900

Mops, Biological Buffer

100g

44.00

Pipes, Good's Buffer Crystalline

Please see listing in Chemicals for Microscopy.

Pipes Buffer Solution, 0.3M

Aqueous PIPES solution adjusted by 0.1N Sodium Hydroxide to pH 5.5-6.0.

0-5°C

11610

Pipes Buffer Solution, 0.3M

450ml

24.00

Sodium Cacodylate Buffer

Sabatini, et. al., J. Cell Biol., 17,19 (1963).
0.2M pH 7.4 (or specify at time of order).

0-5°C	11650	Sodium Cacodylate Buffer	225ml	22.00
0-5°C	11652	Sodium Cacodylate Buffer	450ml	34.00

Sorensen's Phosphate Buffer

Ingredients:
Sodium Phosphate, dibasic (Na₂HPO₄xH₂O)
Potassium Phosphate, monobasic (KH₂PO₄)
Standard 0.15M, pH 7.4 is supplied. Osmolarity is 300 mosmols.
Shipping Conditions: Ambient Temperature
Maximum shelf life after receipt: 1 month

0-5°C	11682-05	Sorensen's Phosphate Buffer, 0.15M	500ml	18.00
0-5°C	11682-10	Sorensen's Phosphate Buffer, 0.15M	1 L	26.00

Sorensen's Phosphate Buffer

(Phosphate Mixed Sodium Salts)
Sorensen, see Dawson et. al, (1969)
Sodium Phosphate, dibasic (Na₂HPO₄xH₂O)
Sodium Phosphate, monobasic (NaH₂PO₄xH₂O)
0.2M, pH range 5.8 - 8.0
Shipping Conditions: Ambient Temperature
Maximum shelf life after receipt: 3 months

The osmolarity of a 0.1 M at a pH 7.2 is 226 mosmols; with the addition of 0.18 M of sucrose to the solution, the osmolarity raises to 425 mosmols.
Standard pH 7.2; 0.2M is supplied. Otherwise, specify pH when ordering.

0-5°C	11600-05	Sorensen's Phosphate Buffer, pH 7.2	500ml	17.00
0-5°C	11600-10	Sorensen's Phosphate Buffer, pH 7.2	1 L	26.00

Sym-Collidine Buffer Kit

2,4,6-Trimethylpyridine
N:C(CH₃)CH:C(CH₃)CH:CCH₃   CAS #108-75-8   F.W. 121.18
Bennett & Luft., Biophys & Biochem. Cytol., 6,113 (1959).

A very stable buffer, used with Osmium tetroxide; provides excellent fixation, better stability and buffering capacity. The pH can be adjusted by varying the amount of hydrochloric acid in the final volume of 200ml. Preparation Instructions: Pour 9ml of 2N HCl; add 5.34ml of Sym-Collidine, mix well and add distilled water up to 200ml for a pH 7.4 solution.

Set consists of:

• 1 amp 5.34ml Sym Collidine
• 1 amp 9.0ml of 2N HCl

0-5°C	11500	Sym-Collidine Buffer Kit	One set	20.00
0-5°C	11510	Sym-Collidine Buffer	Box/5 sets	75.00

Sym-Collidine, Reagent

2,4,6-Collidine; 2,4,6-Trimethylpyridine
(C₅H₂N)(CH₃)₃   CAS #108-75-8   F.W. 121.18
Assay: 99% Specific Gravity: 0.9170

RT

11520

Sym-Collidine, Reagent

100ml

84.00

Tris, (hydroxymethyl) aminomethane, Crystalline

Please see listing in Chemicals for Microscopy.

Tris, (hydroxymethyl) aminomethane maleate

Tris Maleate Buffer
0.2M pH range 6.4-8.0

0-5°C	11730	Tris Maleate Buffer	250ml	16.00
0-5°C	11740	Tris Maleate Buffer	500ml	24.00

Universal Immunoassay Buffer™, 10X

Immunoassay buffer is used as a 1X  in all immunoassays (immunofluorescence, IHC, ELISA, WB etc.) as a washing buffer  and it can be used as a dilution buffer for antibodies; however it does not contain any carrier proteins.

This buffer also does not contain any phosphate, sodium azide or mercury as preservatives and can be used for dilution of all antibodies, including peroxidase, antibodies to phosphoproteins. It is  not suitable for dilution of antibodies to S100 proteins.

Reagent Supplied: 10X Buffer

Application: Dilute this 10X buffer 10 times with DI water. (90 ml water + 10 ml this buffer – mixed well). Wash 3-5 times with this buffer in between the steps of your immunoassay protocol.

Storage: Room temperature

RT	25880-01	Universal Immunoassay Buffer, 10X	100 ml	40.00
RT	25880-10	Universal Immunoassay Buffer, 10X	1 L	85.00

Universal Antibody Dilution Buffer™

This ready-to-use Universal Antibody Dilution Buffer is designed for dilution of antibodies in all immunoassays, including immunofluorescence, IHC, ELISA, WB. This buffer does not contain any mammalian proteins, phosphate, sodium azide, or mercury preservatives and can be used for dilution of all antibodies, including peroxidase, and antibodies to phophoproteins. However, it is not suitable for dilution of antibodies to S100 proteins.

Reagent Supplied: Ready to use buffer

Application: Dilute antibodies, primary or secondary as recommended in the protocol by the manufacturer of these products.

Storage: 2–8°C. Diluted antibodies can be stored at 2–8°C for several weeks, depending upon the stability of antibodies.

2–8°C	25885-01	Universal Antibody Dilution Buffer	100 ml	40.00
2–8°C	25885-05	Universal Antibody Dilution Buffer	500 ml	99.00

Cacodylic to Durcupan