Cacodylic Acid, Free Acid

(Dimethylarsinic Acid)
(CH₃)₂AsO₂H   F.W. 137.99   CAS #75-60-5
Assay 99.5%

RT	12200		25g	36.00
RT	12201		100g	90.00

Cacodylic Acid- Sodium Salt, TrihydrateSodium Cacodylate

Sodium dimethyl arsenate
C₂H₆AsNaO₂^.3H₂O   F.W. 214.02   CAS #124-65-2

Certificate of Analysis:

Identity		Conforming
Appearance		Small Crystals
Color		White
Odor		None
Aqueous solution (2g+10ml)		Colorless
Assay %		99.52
Loss on drying %		25.39
Chlorides %		0.0050
Sulfates %		0.0150
Inorganic Arsenic %		0.0030

RT	12300		100g	73.00
RT	12300-25		25g	26.00
RT	12310		500g	350.00

Cadmium Iodine, Crystal, Reagent, A.C.S.

CdI₂   F.W. 366.21   CAS #7790-80-9
Assay min. 99.0%
Used for negative staining.
m.p. 387°C
Possible Carcinogen.

RT	12320		25g	14.00
RT	12321		100g	50.00

Calcium Chloride, Dihydrate, Reagent, A.C.S.

CaCl₂·2H₂O   F.W. 147.02   CAS #10035-04-8

RT

12340

100g

17.00

Carbohydrazide

CO(NHNH₂)₂   F.W. 90.08   CAS #497-18-7
m.p. 152-153°C
For GACH embedding kit.
A water-miscible, lipid-retaining, embedding polymer for E.M.. Heckman, et. al., Ultrastruct. Res., 42,156 (1973).

RT

12350

25g

16.00

Carbon Adhesives

Please see listing in Mounting Supplies for Scanning Electron Microscopy

Carbowax Polyethylene Glycol

H(OCH₂CH₂)nOH CAS #2532-68-3
An embedding medium for microscopy and histochemistry.

	Cat#	Carbowax	Average M.W.	Density @20°C @60°C	Melting /Freezing range, °C	Pack	Price
RT	19700	PEG 200 viscous liquid	190-210	1.2390 1.0922	set to glass <-65	450ml	20.00
RT	19710	PEG 300 viscous liquid	285-315	1.1250 1.0928	-15 to -8 4	50ml	20.00
RT	19720	PEG 400 viscous liquid	380-420	1.1254 1.0930	4 to 8	450ml	20.00
RT	19730	PEG 600 viscous liquid	570-630	1.1257 1.0930	20 to 25	450ml	20.00
RT	19740	PEG 1000 Waxy	950-1050	— 1.0926	37 to 40	1 kilo	65.00
RT	19750	PEG 1450 Waxy	1300-1600	— 1.0919	43 to 46	1 kilo	65.00
RT	19760	PEG 3350 Waxy	3000-3700	— 1.0926	54 to 58	1 kilo	65.00
RT	19770	PEG 8000 Flakes	7000-9000	— 1.0845	60 to 63	1 kilo	65.00

Gao, K.X., (1993). Polyethylene Glycol as an Embedment for Microscopy and Histochemistry. CRC Press; ISBN# 0-8493-4323-2.

Articles of Interest

The Use of Polyethylene Glycol; Carbowax

Cardolite® NC-513

CH₂OCHCH₂OC₆H₄C₁₅H₂₇   F.W. 356   W.P.E. 490
15-M-Phenylglycidyl-(n-pentadecca-8,11-dienyl)
CAS #106-89-8
Specific Gravity: 0.97 at 24°C
A Long-chain, monofunctional epoxide; stable when mixed with resins.
Viscosity 50 cps at 25°C.

RT

12400

225ml

10.00

Cardolite® is a registered trademark of Cardolite Corporation.

Technical Tip

The Use of Flexibilizers and Plasticizers

Carmine, Certified, C.I.#75470

Alum Lake of Carminic acid
(Natural Red 4)
CAS # 1390-65-4
Solubility: 0.0% Water; 0.2% Alcohol; 0.5% Cellosolve; 4.0% Glycol; 0.0% Xylene.

RT

12410

10 g

26.00

Cedar Wood Oil

CAS #8000-27-9
Refractive Index @ 20°C 1.504+/-.002
For clearing.

RT

12420

100ml

30.00

Cedar Wood Oil

CAS #8000-27-9
Refractive Index @ 20°C 1.515-1.520
Used as an immersion oil.

RT

12430

100ml

32.00

Certified Dyes For Biological Staining

Please see Stains Chart

Chloroform, Reagent, A.C.S.

CHCl₃   FW 119.38   CAS #67-66-3
A.C.S. Specifications:

Color (APHA)	10
Density @ 25°C	1.471-1.476
Residue after Evaporation	0.001%
Acetone & Aldehyde (about 0.00%).	To Pass Test
Acid & Chloride	To Pass Test
Free Chlorine (Cl).	To Pass Test
Lead (Pb)	0.05ppm
Subs. darkened by H2SO4	To Pass Test
Suitability for Dithizone Test.	To Pass Test

RT	12540		450ml	15.00
RT	12541		4x450ml	68.00

Chloroform, Glass Distilled

Specifications:

UV Absorption - 1 cm cell vs H2O
wavelength (nm)	290	270	260	255	245
A	0.01	0.02	0.05	0.15	1.0
Assay					99.7% min.
Evaporation Residue					3ppm max.
Water					0.03% max.

RT	12550		100ml	8.00
RT	12551		4x100ml	27.00

Chromic Acid

Reagent, Crystals, A.C.S.
CrO₃   FW 99.99   CAS #1333-82-0
A.C.S. Specifications:

Chloride (Cl)	0.005%
Insoluble Matter	0.01%
Nitrate (NO3)	0.05%
Sodium (Na)	0.2%
Iron, Aluminum, Barium.	0.03%
Sulfate (SO4)	0.05%
Assay	98%

RT

12555

100g

40.00

Citrate Buffer

Please see listing in Prepared Buffers.

Citric Acid, Anhydrous, Reagent, A.C.S.

C₆H₈O₇   FW 192.13   CAS #77-92-9

A.C.S. Specifications:

Assay	Min. 99.5%
Insoluble Matters	0.005%
Residue after Ignition	0.02%
Chloride	0.001%
Oxalate (C2O4) (about 0.05%)	To Pass Test
Phosphate	0.001%
Sulfate	0.002%
Heavy Metal (as Pb)	2ppm
Subs. carbonized by hot H2SO4	To Pass Test
Iron	3ppm

RT

12480

Citric Acid, Anhydrous,

500g

26.00

Cobalt Napthenate Solution

(55% Cobalt Napthenate; CAS#61789-51-3 and 45% Mineral Spirit; CAS #64742-88-7)
Used as an activator for the Vestopal embedding resin.

RT	12500	Cobalt Napthenate Solution	10ml	7.00
RT	12501	Cobalt Napthenate Solution	25ml	15.00

s-Collidine Buffer

Please see listing in Prepared Buffers.

Collodion, 5% Solution, USP

25% Alcohol, 75% Diethyl Ether in Pyroxylin
CAS #9004-70-0

RT	12600	Collodion, 5% Solution, USP	100ml	13.00
RT	12600-30*	Collodion, 5% Solution, USP	30ml	8.00

*We are now offering Collodion in a 30ml bottle to avoid its classification as a hazardous chemical for shipping purposes.

Collodion (Parlodion), 2% in Amyl Acetate

Highly purified nitrocellulose (parlodion strip) in glass distilled amyl acetate. Useful for forming a negative replica to very fine detail. Two types are available: Our sterile formula which is filtered down to 0.45 micron and our non-sterile formula.

RT	12620-00	Non-Filter 2% Collodion in Amyl Acetate	100 ml	28.00
RT	12620-10	Sterile 2% Collodion in Amyl Acetate (Ultra-Pure)	100 ml	49.00
RT	12620-30*	Non-Filter 2% Collodion in Amyl Acetate	30 ml	12.00
RT	12620-34*	Non-Filter 2% Collodion in Amyl Acetate	4 x 30 ml	38.00
RT	12620-50*	Sterile 2% Collodion in Amyl Acetate (Ultra-Pure)	30 ml	17.00
RT	12620-55*	Sterile 2% Collodion in Amyl Acetate (Ultra-Pure)	4 x 30 ml	60.00

*We are now offering Collodion (Parlodian) in a 30ml bottle to avoid its classification as a hazardous chemical for shipping purposes.

Conjugates; ImmunoGold Reagents

Please see ImmunoGold Reagents.

Technical Tip:

Fluctuation from the recommended optiumum temperature when staining can cause overstaining, understaining or destaining.

Staining Solutions

For all of our Staining Solutions.

Cresyl Fast Violet, Certified, C.I.# 51180

(Cresyl Violet Acetate)
C₁₈H₁₅N₃O₃   F.W. 312.34   CAS # 10510-54-0
Solubility: 0.13% water

RT

12780

Cresyl Fast Violet, Certified,

5 g

72.00

Crystal Violet, Certified, C.I. 42555

(Basic Violet 3; Gentian Violet; Methyl Violet 10B; Hexamethyl Violet)
C₂₅H₃₀N₃Cl   F.W. 407.99   CAS #548-62-9
Solubility: 9.0% Water; 8.75% Alcohol; 7.5% Cellosolve; 7.0% Glycol; 0.0% Xylene

RT

12785

Crystal Violet

25 g

21.00

Cuprolinic Blue™

Quinolinic Phthalocyanine 4,11,18, 25-tetraazoniaphthalocyamine tetrakis(methyl sulfate)
C₃₆H₃₆N₆O₁₆S₄Cu   F.W. 1084.5

Used in the study of RNA in the fields of Histochemistry, Histology, and Genetics. Also used to study the molecular composition of the nature TM (Tectorial membrane) by specifically localizing and examining the morphology of Sulfated PGs (Proteoglycans) in the TM.
Peter A.Santi, M. Kathryn Lease, Robert G. Harrison and Eileen M. Wicker. J. of Electron Micro. Tech. 15:293-300 (1990) : Ultrastructure of Proteoglycans in the Tectorial Membrane.

RT	12800	For a 50ml solution	0.1g	184.00
RT	12802	For a 100ml solution	0.2g	366.00

Water Deionized, Reagent Grade A.C.S.

H₂O F.W. 18.02 CAS #7732-18-5
EMS Reagent Grade Water is typically prepared at 18 megohm/cm specific resistance using a reverse osmosis, mixed deionization, activated filtration and final filtration at 0.2 microns.
Color (APHA) <+/-5
Bacteriological purity 0 CFUs/L
Residue after evaporation 10ppm
Coliform negative

RT	22800-01	Deionized Water	1 gal	10.00
RT	22800-05	Deionized Water	5 gal	42.00

DER 736 Resin

CAS #041638-13-5
Specific Gravity: 1.14
A light colored, flexible epoxy.
Epoxide equivalent weight: 175-205.
Used to simplify infiltration in combination with Epon 812 (Embed 812).
Kushida, J. Electron Micro., 16,278 (1964)

RT	13000	DER 736 Resin	225ml	13.00
RT	13001	DER 736 Resin	10x225ml lot	117.00

DER 732 Resin

CAS #026142-30-3
Specific Gravity: 1.06
Used as a flexibilizer in Maraglas 655-epoxy formulation. Relatively low Viscosity (55-100 at 25°C).
Erlandson, J. Cell Bio., 22,704 (1964).

RT

13010

225ml

16.00

DER 332 Resin

CAS #001675-54-3
Specific Gravity : 1.16
A relatively soft embedding resin used when slower polymerization is needed.
Lockwood, Anat. Rec., 150,129 (1964).

RT

13030

225ml

16.00

Technical Tip:

Stability of Special Staining Solutions

DER 332/732 Embedding Kit

Please see listing in Embedding Media Kits.

Dextrose, Anhydrous, Reagent

(D-Glucose)
C₆H₁₂O₆   FW 180.16   CAS #50-99-7

Assay Purity	99.8%
Insolubles	0.005%
Moisture (105ºC)	0.2%
Ash	0.02%
Chloride	0.01%
Sulfate and Sulfite	0.005%
Arsenic	0.00004%
Heavy Metal (as Pb)	0.00005%
Iron	0.00005%
RNAse & DNAse Activity	None Detected

RT

13050

500g

12.50

(DAB), 3,3'-Diaminobenzidine, EM Grade, Purified free-base

(3,3',4,4'-Biphenyltetramin)
C₁₂H₁₄N₄   F.W. 214.27   CAS #91-95-2
m.p. 173°C
Used as an ultrastructural protein tracer for immuno-cytochemistry. A possible carcinogen.

0-5ºC

13060

1g

16.50

(TMB), 3,3,5,5-Tetramethyl Benzidine

Please see (TMB), 3,3,5,5-Tetramethyl Benzidine for more information.

(DAB-HCl), 3,3-Diaminobenzidine Tetra Hydrochloride, EM Grade

C₁₂H₁₄N₄·4HCl   F.W. 360.12   CAS #7411-49-6
m.p. 300°C
Used for histochemical localization of peroxidase with DAB and in immunocytochemistry.
Sternberger, et.al., J. Histochem, Cytochem., 18,215 (1970)

0-5ºC

13080

1g

19.50

We are now offering DAB 4HCl pre-weighed and packed in tablet form, to facilitate your work.
Each tablet contains 10mg. Just break the tablet or tablets in a suitable bottle and add your buffer. All weighing and contact with the solid ( DAB-HCl) is avoided.

0-5ºC	13082		10 tablets/pkg	80.00
0-5ºC	13083		20 tablets/pkg	152.00

(DBP) Dibutyl Phthalate

C₆H₄(COOC₄H₉)₂   F.W. 278.35   CAS #84-74-2
Specific Gravity: 1.049
A plasticizer for epoxy resins.

0-5ºC	13100		225ml	11.00
0-5ºC	13101		Lot of 10x225ml	99.00
0-5ºC	13110		450ml	22.00

1,2-Dichloroethane, Reagent, A.C.S.

(Ethylene Dichloride)
CH₂ClCH₂Cl   F.W. 98.96   CAS #107-06-2
A.C.S. Specifications

Color (A.H.S.A.)	10
Appearance	Clear
Odor	Resembling odor of CHCl
Density @25°C	1.241 -1.251
Boiling Range, Incl. 83.5°C	20°C
Residue after Evaporation	0.002%
Titratable Acid	0.0003 meq/g
Water	0.03%

RT	13250		450ml	18.00
RT	13251		4x450ml	64.00

Diethylene Glycol Distearate

Please see listing in Embedding Media Kits.

RT

13255

500gm

142.00

Digitonin, Crystal, Reagent

C₅₆H₉₂0₂₉   F.W. 1229.34   CAS #11024-24-1
An additive which stabilizes cholesterol 0.2% digitonin in buffer added to Glutaraldehyde fixative may help to stabilize and retain the fine structural localization of free cholesterol and cholesterol esters.
* Okros, I Histochemie 13-91, 1968. Windaus, A. Hoop Seyler's Z. Physio. Chem. 65:110, 1910. J. Cell Bio., 40, 802, 1969.
* Temkin, Robert J. (1993); Microscopy Research and Tech. 26:260-271

RT

13260

1g

180.00

(DMAE), 2-Dimethylaminoethanol

C₄H₁₁NO   F.W. 89.14   CAS #108-01-0
Specific Gravity: 0.883-0.888
b.p. 132-134°C
A curing agent for epoxy resins.

RT

13300

25ml

5.50

(DMA) N,N-Dimethylaminoethyl Methacrylate

C₈H₁₅NO₂   F.W. 157.21   CAS #2867-47-2
Specific Gravity: 0.933
b.p. 76°C
A water soluble monomer.

RT

13350

450ml

15.00

(DMP), 2,2-Dimethoxypropane

(Acetone Dimethyl Acetal)
(CH₃)₂C(OCH₃)₂   F.W. 104.15   CAS #77-76-9
Specific Gravity: 0.849
Used as a dehydration agent.
* Miller, L.L. and Jacks, T.J. (1975). Rapid chemical dehydration of samples for electron microscopic examinations. J. Histochem. Cytochem, 23:107
* Thorpe J.R. and Harvey D.M.R. (1979). Optimization and investigation of the use of 2,2-dimethoxypropane as a dehydration agent for plant tissue in TEM. J. Ultrastrut. Res. ,68:186
* Beckmann H.J. and Dierichs, R. (1982). Lipid extracting properties of 2,2-Dimethoxypropane as revealed by electron microscopy and thin layer chromatography Histochemistry, 76:407

RT	13280		450ml	30.00
RT	13281		4x450ml	108.00

Technical Tip:

The Use of DMP (2,2-Dimethoxy Propane)

DMP-30

(2,4,6-Tri(dimethylaminomethyl) phenol)
F.W. 265.0   CAS #90-72-2
Specific Gravity: 0.973 @ 25°C/25°C
Refractive Index @25°C :1.514
A tertiary amine, used as an accelerator for anhydride cures. One of the most popular accelerators available! Keep as dry as possible. Short shelf life. Use as fresh as possible.

RT	13600		50ml	2.50
RT	13600-SP		50 ml	4.00

N,N-Dimethylaniline

C₆H₅N(CH₃)₂   F.W. 121.18   CAS #121-69-7
Specific Gravity: 0.96 g/ml

RT

13360

100ml

8.00

(DMSO), Dimethyl Sulfoxide, Reagent, A.C.S.

(CH₃)₂SO   FW 78.13   CAS #67-68-5
A.C.S. Specifications:

Appearance	Clear, Colorless
Density (g/ml)@25°C	min. 1.095
Freezing Point	Not below 18.0°C
Residue after evaporation	0.01%
Water	0.2%

RT

13390

450ml

32.00

Technical Tip

Cryo-Protectants

Divinyl Benzene, Mixed Isomers

C₆H₄(CH:CH₂)₂   F.W. 130.19   CAS #1321-74-0
A cross-linking monomer.Inhibited with up to 1,500 ppm p-tert-butyl catechol.
Specific Gravity: 0.91

RT

13450

100ml

12.00

D.D.S.A.

(Dodecenyl Succinic Anhydride)
C₁₆H₂₆O₃   F.W. 266.38   CAS #25377-73-5
Specific Gravity: 1.005

RT	13700		450ml	11.00
RT	13701		Lot 4x450ml