Electron Microscopy Sciences

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Chemicals for Microscopy

Electron Microscopy Sciences

arrow14Cacodylic to Durcupan

arrowCacodylic Acid, Free Acid

(Dimethylarsinic Acid)
(CH3)2AsO2H   F.W. 137.99   CAS #75-60-5
Assay 99.5%

RT 12200   25g 36.00 cart
RT 12201   100g 90.00 cart

Cacodylic Acid- Sodium Salt, TrihydrateSodium CacodylatearrowCacodylic Acid- Sodium Salt, TrihydrateSodium Cacodylate

Sodium dimethyl arsenate
C2H6AsNaO2.3H2O   F.W. 214.02   CAS #124-65-2


Certificate of Analysis:

Identity   Conforming
Appearance   Small Crystals
Color   White
Odor   None
Aqueous solution (2g+10ml)   Colorless
Assay %   99.52
Loss on drying %   25.39
Chlorides %   0.0050
Sulfates %   0.0150
Inorganic Arsenic %   0.0030
RT 12300   100g 73.00 cart
RT 12300-25   25g 26.00 cart
RT 12310   500g 350.00 cart

arrow12Cadmium Iodine, Crystal, Reagent, A.C.S.

CdI2   F.W. 366.21   CAS #7790-80-9
Assay min. 99.0%
Used for negative staining.
m.p. 387°C
Possible Carcinogen.

RT 12320   25g 14.00 cart
RT 12321   100g 50.00 cart

arrow12Calcium Chloride, Dihydrate, Reagent, A.C.S.

CaCl2·2H2O   F.W. 147.02   CAS #10035-04-8

RT 12340   100g 17.00 cart

arrow12Carbohydrazide

CO(NHNH2)2   F.W. 90.08   CAS #497-18-7
m.p. 152-153°C
For GACH embedding kit.
A water-miscible, lipid-retaining, embedding polymer for E.M.. Heckman, et. al., Ultrastruct. Res., 42,156 (1973).

RT 12350   25g 16.00 cart

arrow12Carbon Adhesives

Please see listing in Mounting Supplies for Scanning Electron Microscopy

arrow12 Carbowax Polyethylene Glycol

H(OCH2CH2)nOH CAS #2532-68-3
An embedding medium for microscopy and histochemistry.

  Cat# Carbowax  Average M.W. Density @20°C @60°C Melting
/Freezing
range, °C 
Pack Price  
RT 19700 PEG 200
viscous liquid
190-210 1.2390 1.0922 set to glass
<-65
450ml 20.00 cart
RT 19710 PEG 300
viscous liquid
285-315 1.1250 1.0928 -15 to -8 4 50ml 20.00 cart
RT 19720 PEG 400
viscous liquid
380-420 1.1254 1.0930 4 to 8 450ml 20.00 cart
RT 19730 PEG 600
viscous liquid
570-630 1.1257 1.0930 20 to 25  450ml 20.00 cart
RT 19740 PEG 1000
Waxy
950-1050
1.0926
37 to 40 1 kilo 65.00 cart
RT 19750 PEG 1450
Waxy
1300-1600
1.0919
43 to 46 1 kilo 65.00 cart
RT 19760 PEG 3350
Waxy
3000-3700
1.0926
54 to 58 1 kilo 65.00 cart
RT 19770 PEG 8000
Flakes
7000-9000
1.0845
60 to 63 1 kilo 65.00 cart

Gao, K.X., (1993). Polyethylene Glycol as an Embedment for Microscopy and Histochemistry. CRC Press; ISBN# 0-8493-4323-2.

Articles of Interest

The Use of Polyethylene Glycol; Carbowax

arrow12Cardolite® NC-513

CH2OCHCH2OC6H4C15H27   F.W. 356   W.P.E. 490
15-M-Phenylglycidyl-(n-pentadecca-8,11-dienyl)
CAS #106-89-8
Specific Gravity: 0.97 at 24°C
A Long-chain, monofunctional epoxide; stable when mixed with resins.
Viscosity 50 cps at 25°C.

RT 12400   225ml 10.00 cart
Cardolite® is a registered trademark of Cardolite Corporation.

Technical Tip

The Use of Flexibilizers and Plasticizers

arrow12Carmine, Certified, C.I.#75470

Alum Lake of Carminic acid
(Natural Red 4)
CAS # 1390-65-4
Solubility: 0.0% Water; 0.2% Alcohol; 0.5% Cellosolve; 4.0% Glycol; 0.0% Xylene.

RT 12410   10 g 26.00 cart

arrow12Cedar Wood Oil

CAS #8000-27-9
Refractive Index @ 20°C 1.504+/-.002
For clearing.

RT 12420   100ml 30.00 cart

arrow12Cedar Wood Oil

CAS #8000-27-9
Refractive Index @ 20°C 1.515-1.520
Used as an immersion oil.

RT 12430   100ml 32.00 cart

arrow12Certified Dyes For Biological Staining

Please see Stains Chart

arrow12Chloroform, Reagent, A.C.S.

CHCl3   FW 119.38   CAS #67-66-3
A.C.S. Specifications:

Color (APHA) 10
Density @ 25°C 1.471-1.476
Residue after Evaporation 0.001%
Acetone & Aldehyde (about 0.00%). To Pass Test
Acid & Chloride To Pass Test
Free Chlorine (Cl). To Pass Test
Lead (Pb) 0.05ppm
Subs. darkened by H2SO4  To Pass Test
Suitability for Dithizone Test. To Pass Test
RT 12540   450ml 15.00 cart
RT 12541   4x450ml 68.00 cart

arrow12Chloroform, Glass Distilled

Specifications:

UV Absorption - 1 cm cell vs H2O
wavelength (nm) 290 270 260 255 245
A 0.01 0.02 0.05 0.15 1.0
Assay   99.7% min.
Evaporation Residue   3ppm max.
Water   0.03% max.
RT 12550   100ml 8.00 cart
RT 12551   4x100ml 27.00 cart

arrow12Chromic Acid

Reagent, Crystals, A.C.S.
CrO3   FW 99.99   CAS #1333-82-0
A.C.S. Specifications:

Chloride (Cl) 0.005%
Insoluble Matter 0.01%
Nitrate (NO3) 0.05%
Sodium (Na) 0.2%
Iron, Aluminum, Barium. 0.03%
Sulfate (SO4) 0.05%
Assay 98%
RT 12555   100g 40.00 cart

arrow12Citrate Buffer

Please see listing in Prepared Buffers.

arrow12 Citric Acid, Anhydrous, Reagent, A.C.S.

C6H8O7   FW 192.13   CAS #77-92-9

A.C.S. Specifications:

Assay Min. 99.5%
Insoluble Matters 0.005%
Residue after Ignition 0.02%
Chloride 0.001%
Oxalate (C2O4) (about 0.05%) To Pass Test
Phosphate 0.001%
Sulfate 0.002%
Heavy Metal (as Pb) 2ppm
Subs. carbonized by hot H2SO4  To Pass Test
Iron 3ppm
RT 12480 Citric Acid, Anhydrous, 500g 26.00 cart

arrow12Cobalt Napthenate Solution

(55% Cobalt Napthenate; CAS#61789-51-3 and 45% Mineral Spirit; CAS #64742-88-7)
Used as an activator for the Vestopal embedding resin.

RT 12500 Cobalt Napthenate Solution 10ml 7.00 cart
RT 12501 Cobalt Napthenate Solution 25ml 15.00 cart

arrow12s-Collidine Buffer

Please see listing in Prepared Buffers.

arrow12 Collodion, 5% Solution, USP

25% Alcohol, 75% Diethyl Ether in Pyroxylin
CAS #9004-70-0

RT 12600 Collodion, 5% Solution, USP 100ml 13.00 cart
RT 12600-30* Collodion, 5% Solution, USP 30ml 8.00 cart

*We are now offering Collodion in a 30ml bottle to avoid its classification as a hazardous chemical for shipping purposes.

arrow12 Collodion (Parlodion), 2% in Amyl Acetate

Highly purified nitrocellulose (parlodion strip) in glass distilled amyl acetate. Useful for forming a negative replica to very fine detail. Two types are available: Our sterile formula which is filtered down to 0.45 micron and our non-sterile formula.

RT 12620-00 Non-Filter 2% Collodion in Amyl Acetate 100 ml 28.00 cart
RT 12620-10 Sterile 2% Collodion in Amyl Acetate (Ultra-Pure) 100 ml 49.00 cart
RT 12620-30* Non-Filter 2% Collodion in Amyl Acetate 30 ml 12.00 cart
RT 12620-34* Non-Filter 2% Collodion in Amyl Acetate 4 x 30 ml 38.00 cart
RT 12620-50* Sterile 2% Collodion in Amyl Acetate (Ultra-Pure) 30 ml 17.00 cart
RT 12620-55* Sterile 2% Collodion in Amyl Acetate (Ultra-Pure) 4 x 30 ml 60.00 cart

*We are now offering Collodion (Parlodian) in a 30ml bottle to avoid its classification as a hazardous chemical for shipping purposes.

arrow12Conjugates; ImmunoGold Reagents

Please see ImmunoGold Reagents.

Technical Tip:

Fluctuation from the recommended optiumum temperature when staining can cause overstaining, understaining or destaining.

arrow12Staining Solutions

For all of our Staining Solutions.

arrow12Cresyl Fast Violet, Certified, C.I.# 51180

(Cresyl Violet Acetate)
C18H15N3O3   F.W. 312.34   CAS # 10510-54-0
Solubility: 0.13% water

RT 12780 Cresyl Fast Violet, Certified, 5 g 72.00 cart

arrow12Crystal Violet, Certified, C.I. 42555

(Basic Violet 3; Gentian Violet; Methyl Violet 10B; Hexamethyl Violet)
C25H30N3Cl   F.W. 407.99   CAS #548-62-9
Solubility: 9.0% Water; 8.75% Alcohol; 7.5% Cellosolve; 7.0% Glycol; 0.0% Xylene

RT 12785 Crystal Violet 25 g 21.00 cart

arrow12Cuprolinic Blue™

Quinolinic Phthalocyanine 4,11,18, 25-tetraazoniaphthalocyamine tetrakis(methyl sulfate)
C36H36N6O16S4Cu   F.W. 1084.5

Used in the study of RNA in the fields of Histochemistry, Histology, and Genetics. Also used to study the molecular composition of the nature TM (Tectorial membrane) by specifically localizing and examining the morphology of Sulfated PGs (Proteoglycans) in the TM.
Peter A.Santi, M. Kathryn Lease, Robert G. Harrison and Eileen M. Wicker. J. of Electron Micro. Tech. 15:293-300 (1990) : Ultrastructure of Proteoglycans in the Tectorial Membrane.

RT 12800 For a 50ml solution 0.1g 184.00 cart
RT 12802 For a 100ml solution 0.2g 366.00 cart

arrowWater Deionized, Reagent Grade A.C.S.

H2O F.W. 18.02 CAS #7732-18-5
EMS Reagent Grade Water is typically prepared at 18 megohm/cm specific resistance using a reverse osmosis, mixed deionization, activated filtration and final filtration at 0.2 microns.
Color (APHA) <+/-5
Bacteriological purity 0 CFUs/L
Residue after evaporation 10ppm
Coliform negative

RT 22800-01 Deionized Water 1 gal 10.00 cart
RT 22800-05 Deionized Water 5 gal 42.00 cart

arrow12DER 736 Resin

CAS #041638-13-5
Specific Gravity: 1.14
A light colored, flexible epoxy.
Epoxide equivalent weight: 175-205.
Used to simplify infiltration in combination with Epon 812 (Embed 812).
Kushida, J. Electron Micro., 16,278 (1964)

RT 13000 DER 736 Resin 225ml 13.00 cart
RT 13001 DER 736 Resin 10x225ml lot 117.00 cart

arrow12DER 732 Resin

CAS #026142-30-3
Specific Gravity: 1.06
Used as a flexibilizer in Maraglas 655-epoxy formulation. Relatively low Viscosity (55-100 at 25°C).
Erlandson, J. Cell Bio., 22,704 (1964).

RT 13010   225ml 16.00 cart

arrow12DER 332 Resin

CAS #001675-54-3
Specific Gravity : 1.16
A relatively soft embedding resin used when slower polymerization is needed.
Lockwood, Anat. Rec., 150,129 (1964).

RT 13030   225ml 16.00 cart

Technical Tip:

Stability of Special Staining Solutions

arrow12DER 332/732 Embedding Kit

Please see listing in Embedding Media Kits.

arrow12Dextrose, Anhydrous, Reagent

(D-Glucose)
C6H12O6   FW 180.16   CAS #50-99-7

Assay Purity 99.8%
Insolubles 0.005%
Moisture (105ºC) 0.2%
Ash 0.02%
Chloride 0.01%
Sulfate and Sulfite 0.005%
Arsenic 0.00004%
Heavy Metal (as Pb) 0.00005%
Iron 0.00005%
RNAse & DNAse Activity None Detected
RT 13050   500g 12.50 cart

arrow12(DAB), 3,3'-Diaminobenzidine, EM Grade, Purified free-base

(3,3',4,4'-Biphenyltetramin)
C12H14N4   F.W. 214.27   CAS #91-95-2
m.p. 173°C
Used as an ultrastructural protein tracer for immuno-cytochemistry. A possible carcinogen.

0-5ºC 13060   1g 16.50 cart

arrow12(TMB), 3,3,5,5-Tetramethyl Benzidine

Please see (TMB), 3,3,5,5-Tetramethyl Benzidine for more information.

arrow12(DAB-HCl), 3,3-Diaminobenzidine Tetra Hydrochloride, EM Grade

C12H14N4·4HCl   F.W. 360.12   CAS #7411-49-6
m.p. 300°C
Used for histochemical localization of peroxidase with DAB and in immunocytochemistry.
Sternberger, et.al., J. Histochem, Cytochem., 18,215 (1970)

0-5ºC 13080   1g 19.50 cart

We are now offering DAB 4HCl pre-weighed and packed in tablet form, to facilitate your work.
Each tablet contains 10mg. Just break the tablet or tablets in a suitable bottle and add your buffer. All weighing and contact with the solid ( DAB-HCl) is avoided.

0-5ºC 13082   10 tablets/pkg 80.00 cart
0-5ºC 13083   20 tablets/pkg 152.00 cart

arrow12(DBP) Dibutyl Phthalate

C6H4(COOC4H9)2   F.W. 278.35   CAS #84-74-2
Specific Gravity: 1.049
A plasticizer for epoxy resins.

0-5ºC 13100   225ml 11.00 cart
0-5ºC 13101   Lot of 10x225ml 99.00 cart
0-5ºC 13110   450ml 22.00 cart

arrow121,2-Dichloroethane, Reagent, A.C.S.

(Ethylene Dichloride)
CH2ClCH2Cl   F.W. 98.96   CAS #107-06-2
A.C.S. Specifications

Color (A.H.S.A.) 10
Appearance Clear
Odor Resembling odor of CHCl
Density @25°C 1.241 -1.251
Boiling Range, Incl. 83.5°C 20°C
Residue after Evaporation 0.002%
Titratable Acid 0.0003 meq/g
Water 0.03%
RT 13250   450ml 18.00 cart
RT 13251   4x450ml 64.00 cart

arrow12Diethylene Glycol Distearate

Please see listing in Embedding Media Kits.

RT 13255   500gm 142.00 cart

arrow12Digitonin, Crystal, Reagent

C56H92029   F.W. 1229.34   CAS #11024-24-1
An additive which stabilizes cholesterol 0.2% digitonin in buffer added to Glutaraldehyde fixative may help to stabilize and retain the fine structural localization of free cholesterol and cholesterol esters.
* Okros, I Histochemie 13-91, 1968. Windaus, A. Hoop Seyler's Z. Physio. Chem. 65:110, 1910. J. Cell Bio., 40, 802, 1969.
* Temkin, Robert J. (1993); Microscopy Research and Tech. 26:260-271

RT 13260   1g 180.00 cart

arrow12(DMAE), 2-Dimethylaminoethanol

C4H11NO   F.W. 89.14   CAS #108-01-0
Specific Gravity: 0.883-0.888
b.p. 132-134°C
A curing agent for epoxy resins.

RT 13300   25ml 5.50 cart

arrow12(DMA) N,N-Dimethylaminoethyl Methacrylate

C8H15NO2   F.W. 157.21   CAS #2867-47-2
Specific Gravity: 0.933
b.p. 76°C
A water soluble monomer.

RT 13350   450ml 15.00 cart

arrow12(DMP), 2,2-Dimethoxypropane

(Acetone Dimethyl Acetal)
(CH3)2C(OCH3)2   F.W. 104.15   CAS #77-76-9
Specific Gravity: 0.849
Used as a dehydration agent.
* Miller, L.L. and Jacks, T.J. (1975). Rapid chemical dehydration of samples for electron microscopic examinations. J. Histochem. Cytochem, 23:107
* Thorpe J.R. and Harvey D.M.R. (1979). Optimization and investigation of the use of 2,2-dimethoxypropane as a dehydration agent for plant tissue in TEM. J. Ultrastrut. Res. ,68:186
* Beckmann H.J. and Dierichs, R. (1982). Lipid extracting properties of 2,2-Dimethoxypropane as revealed by electron microscopy and thin layer chromatography Histochemistry, 76:407

RT 13280   450ml 30.00 cart
RT 13281   4x450ml 108.00 cart

Technical Tip:

The Use of DMP (2,2-Dimethoxy Propane)

arrow12DMP-30

(2,4,6-Tri(dimethylaminomethyl) phenol)
F.W. 265.0   CAS #90-72-2
Specific Gravity: 0.973 @ 25°C/25°C
Refractive Index @25°C :1.514
A tertiary amine, used as an accelerator for anhydride cures. One of the most popular accelerators available! Keep as dry as possible. Short shelf life. Use as fresh as possible.

RT 13600   50ml 2.50 cart
RT 13600-SP   50 ml 4.00 cart

arrow12N,N-Dimethylaniline

C6H5N(CH3)2   F.W. 121.18   CAS #121-69-7
Specific Gravity: 0.96 g/ml

RT 13360   100ml 8.00 cart

arrow12(DMSO), Dimethyl Sulfoxide, Reagent, A.C.S.

(CH3)2SO   FW 78.13   CAS #67-68-5
A.C.S. Specifications:

Appearance Clear, Colorless
Density (g/ml)@25°C min. 1.095
Freezing Point Not below 18.0°C
Residue after evaporation 0.01%
Water 0.2%
RT 13390   450ml 32.00 cart

Technical Tip

Cryo-Protectants

arrow12Divinyl Benzene, Mixed Isomers

C6H4(CH:CH2)2   F.W. 130.19   CAS #1321-74-0
A cross-linking monomer.Inhibited with up to 1,500 ppm p-tert-butyl catechol.
Specific Gravity: 0.91

RT 13450   100ml 12.00 cart

arrow12D.D.S.A.

(Dodecenyl Succinic Anhydride)
C16H26O3   F.W. 266.38   CAS #25377-73-5
Specific Gravity: 1.005

RT 13700   450ml 11.00 cart
RT 13701   Lot 4x450ml