EA Counterstain, Gill’s Modified Solution

For use with Papanicolaou stain; a general tissue stain. Ready-to-use.

RT	26050-05	EA Counterstain, Gill’s Modified	500ml	36.00	Add to Cart
RT	26050-06	EA Counterstain, Gill’s Modified	1 L	66.00	Add to Cart

EARLE's Balanced Salt Solutions - EBSS

All of our Earle’s Balanced Salt Solution contains: Potassium Chloride, Sodium Chloride, Sodium Phosphate Monobasic, and D-glucose. Some formulations may contain additional components. Use the table below as a quick reference for identifying the one that best suits your application. All of the solutions are prepared 1X strength.

Ingredients	F.W.	Formula I	Formula II	Formula III
KCl	74.56	X	X	X
NaCl	58.44	X	X	X
NaH2PO4. H2O	137.99	X	X	X
D-Glusose	180-16	X	X	X
MgSO4. 7H2O	264.48	X	-----	----
CaCl2,, 2H2O	147.02	X	-----	----
NaHCO3	84.01	----	-----	----
Phenol Red		----	X	X

RT	11765-05	EBSS, Formula I	500ml	15.00
RT	11765-10	EBSS, Formula I	1 L	20.00
RT	11766-05	EBSS, Formula II	500ml	15.00
RT	11766-10	EBSS, Formula II	1 L	20.00
RT	11767-05	EBSS, Formula III	500ml	15.00
RT	11767-10	EBSS, Formula III	1 L	20.00

EDTA, Disodium Salt, Dihydrate, A.C.S.

(Ethylenediamine Tetraacetic acid)
Na₂C₁₀H₁₄O₈·2H₂O   F.W. 372.20   CAS #6381-92-6
A.C.S. Specifications:

Assay.	99+%
Insoluble matter	max 0.005%
Nitrilotriacetic acid	max 0.1%
Heavy Metal (as Pb)	max 0.005%
Iron	max 0.01%

Organic chelating for staining glutaraldehyde fixed tissues, pre-stained with uranyl acetate, followed by lead citrate. Bernard, W., J. Ultrastruct. Res., 27. 250 (1969)

RT

15200

100g

31.00

Ehrlich’s Hematoxylin

Stain selectivity for nuclei. Ready-to-use.

RT	26040-05	Ehrlich’s Hematoxylin	500ml	28.00	Add to Cart
RT	26040-06	Ehrlich’s Hematoxylin	1 L	50.00	Add to Cart

EMbed 812

CAS # N.A.
Specific Gravity: 1.08-1.10
EMbed 812 has the same characteristics as Epon 812, physically, and provides the same results in preservation, handling, curing and sectioning. EMbed 812 may cause etching on selected plastics. All formulas for mixing and using Epon 812 can be applied to EMbed 812, e.g. Luft's procedure, Mollenhauer's and EPON-ARALDITE mixtures.

RT	14900		450ml	25.00
RT	14901		4x450ml	90.00

EMbed 812 Embedding Kit

Please see listing in Embedding Media Kits.

RT

14120

kit

58.00

EMbed 812 / DER 736 Embedding Kit

Please see listing in Embedding Media Kits.

RT

14130

kit

60.00

Technical Tip

An Epon-812 Replacement

Entellan®

Please see listing in Adhesives and Mountants

RT	14800		100ml	50.00
RT	14802		500ml	195.00

Eosin Y, Certified, C.I. #45380

(Acid Red 87, Eosin Yellowish)
C_2cH₆Br₄Na₂O₅   F.W. 691.88   CAS #173772-87-1

Solubility: 44.0% Water; 2.0% Alcohol; 25.0% Cellosolve; 27.5% Glycol; 0.0% Xylene.
Used with Hematoxylin for showing cell nuclei in embryos and kidneys. Conn, "Biological Stain" 9th ed., The Williams and Wilkens Co., Baltimore 1977, pg. 7.

Component of Wright's stain for blood corpuscles. Wright, J. Med. Res., 7, 138 (1902)
For staining bone marrow to show cell morphology. Bech, "Lab. Manual of Hematological Tech.", W. B. Saunders Co., Phila., 1908, p. 233

RT	14850		10g	8.25
RT	14851		25g	14.00

EPO-Fix Embedding Media

Please see listing in Embedding Media Kits.

Epon-812 Resin

Please see listing in Embedding Media Kits.

Epon-815 Resin;

CAS # NA
Specific Gravity: 1.13
An aromatic resin.

RT

14910

450ml

22.00

EMS Cured Epoxy Remover

Studies show that in order to get good results in positive staining of sections, the removal of cured epoxy (embedding material) as well as the reduced-osmium (fixation materials) from the sections is necessary; especially when double fixation with an Aldehyde and Osmium tetroxide is employed to process specimens for E.M. We offer a CURED-EPOXY-REMOVER kit, which removes the polymerized resin as well as the reduced-osmium. The structure of the resin free section will remain unchanged.

RT

14942

kit

75.00

Technical Tip

Re-Embedding of Tissue Poorly Embedded

Epoxy Tissue Stain, Ready-to-use

Toluidine Blue and Basic Fuchsin. For staining semi thin sections of epoxy embedded tissues.
Spurlock, B.O., (1966) et al., Am. J. of Clin. Path.

RT

14950

100ml

20.00

Erythrosin B, Certified, C.I. #45430

(Acid Red 51, Erythrosin extra Bluish; Eosin J; Iodeosin B; Pyrosin B)
C₂₀H₆O₅I₄Na₂   F.W. 879.87   CAS #16423-68-0
Solubility: 10.0% Water; 5.0% Alcohol; 7.0% Cellosolve; 8.25% Glycol; 0.0% Xylene.

RT

15010

10 g

23.00

ERL 4221-An ERL-4206 Replacement

Due to the supply shortage of ERL-4206 ERL-4221 has now been specially formulated to replace it. ERL 4206 is one of the main components of the low viscosity (Dr. Spur) embedding resin kit.

Specifications:

ERL-4221 is a Cycloaliphatic Epoxide Resin.
Specific Gravity (H2O=1): 1.173 @ 20°C/20°C
Flash Point – Closed Cup: 118°C (245°F)
Boiling Point (760mm/Hg):>250°C (>482°F)

RT	15004	ERL-4221	225ml	20.00
RT	15005	ERL-4221	4 X 225ml	72.00

Ethyl Alcohol, Specially Denatured

C₂H₅OH   F.W. 46.07   CAS #64-17-5
Blended 95% Ethanol and 5% Methanol
Suitable for all routine histology/cytology preparations.
Specifications:
Evaporation Residue 5ppm max.
Water 0.1%
Filtered SDA-3A. 95%

RT	15050		1qt (946ml)	21.00
RT	15051		4x1qt	76.00

Ethyl Alcohol, Anhydrous

(Ethanol) - 200 Proof
CH₃CH₂OH   F.W. 46.07   CAS # 64-17-5
Specifications:

Specific Gravity	<0.7942
Purity	>99.9%
Acidity	<0.002%
Water (Karl Fisher)	<0.1%
Non-Volatile Matter	<0.0025%

RT	15055		450ml	18.00*
RT	15056		4x450ml	68.00*
RT	15058		1 liter	32.00

*Tax has already been paid

Ethylene Dichloride, Reagent, A.C.S. 1,2-Dichloroethane

Please see 1,2-Dichloroethane.

RT	13250		450ml	18.00
RT	13251		4x450ml	5648.00

Ethylene Glycol Dimethacrylate

(Ethylene dimethacrylate)
C₁₀H₁₄O₄   F.W. 198.22   CAS #97-90-5
Specific Gravity: 1.053
b.p. 66-68°C
Inhibited with 100 ppm HQ. (Hydroquinone)

0-5ºC

15300

225ml

36.00

Ethylene Glycol Monoethyl Ether, Purified

(Cellosolve; 2-Ethoxyethanol; Ethyl Glycol)
HOCH₂CH₂OCH₂CH₃   F.W. 90.12   CAS #110-80-5
bp 133-135°C
Specific Gravity @20°C: 0.931
Assay >99.5%
Water <0.1%
Residue after evaporation <0.001%
Suitable for histological use.

0-5ºC	15310-01		1L	37.50
0-5ºC	15310-06		6x1L	196.00

Eukitt™ Mounting Media

Please see listing in Adhesives and Mountants.

Farnesol

(3,7,11-Trimethyl-2,6,10-dodecatrien-1-ol)
C₁₅H₂₆O   F.W. 222.57   CAS #4602-84-0
Specific Gravity: 0.886
Mixture of Stereoisomers: Approx. 90% Synthetic

0-5ºC

15340

10ml

23.00

Fast Green FCF, Certified, C.I. 42053

(Food Green 3)
C₃₇H₃₄O₁₀N₂S₃Na₂   F.W.808.86   CAS #2353-45-9
Solubility:4.0% Water; 9.0% Alcohol; 8.0% Cellosolve; 6.25% Glycol; 0.0% Xylene

The main advantage of this dye is its resistance to fading. Mammalian tissue stain for visualizing collagen, muscle, cystoplasma, cells and cornified epithelium. Kornhauser, Stain Tech., 18,95 (1943); 20, 23 (1945); Lillie, Arch. Path., 29, 705 (1940). Modified Feulgen stain for visualizing chromosomes in root tips and other meristematic tissue. DeTomasi, Stain Tech., 11, 137 (1936). Used in plant histology. O. Conn, "Biological Stains" 9th ed., Williams & Wilkens Co., Baltimore, 1977, pp. 252-3; Haynes, Stain Tech., 3, 40 (1928).

RT

15500

5g

22.00

Fast Green Solutions

Please see listing in Prepared Staining Solutions.

Ferric Chloride, Hexahydrate, Reagent, A.C.S.

FeCl₃·6H₂O   F.W. 270.30   CAS #13478-10-9

Assay	97.0-102%
Insoluble Matters.	0.01%
Nitrate (about 0.001%)	To Pass Test
Phosphorous Compound	0.01%
Sulfate	0.01%
Arsenic	0.002%
Copper	0.003%
Zinc	0.003%

References for use: Membrane stains

• Gasic, G., Berwick et al (1963). Hale stain for sialic acid containing mucins. J. Cell Biol., 19:223
• Gasic, G., Berwick, et al (1968). Positive and Negative colloidal iron as cell surface electron stains. Lab. Invest., 18:63
• Blanquet, P.R. and Loiez, A. (1974). Colloidal iron used at pHs lower than 1 as electron stain for surface proteins. J. Histochem. Cytochem., 22:368
• Matukafs, V.J., Panner et al (1967). Studies on ultrastructural identification and distribution of protein-polysaccharides in cartilage matrix. J. Cell Biol., 32:365
• Benedetti, E.L. and Emmelot, P. (1967). Studies on plasma membranes IV. The Ultrastructural localization and content of sialic acid in plasma membranes isolated from rat liver and hepatoma. J. Cell Sci., 2:499
• Rowley, J.R. (1971). Resolution of channels in the exine by translocation of colloidal iron. Pro. 29th Ann. EMSA Meet., p.352. Claitor's Pub. Division, Baton Rouge, LA
• Nicolson, G.L. (1973). Anionic sites of human erythrocyte membranes.I. Effects of trypsin, phospholipase C, and pH on the topography of bound positively charged colloidal particles. J. Cell Biol., 57:373
• Hendy, R. (1971). Electron microscopy of lipofucsin pigment stained by the Schmorl and Fontana technique. Histochemie, 26:311.

RT

15510

Ferric Chloride, Hexahydrate, Reagent,

500g

45.00

Ferric Chloride Solutions

Please see listing in Reagents for Histology.

Ferritin Cationized:

CAS #9007-73-2

This polycationic derivative of ferritin is useful in labeling negative charges on cell surfaces and to study membrane structure. Cationized ferritin is prepared by coupling ferritin from horse spleen with N,N-dimethyl-1,3-propanediamine (DMPA). Sterile filtered solution in 0.15M NaCl.
Ferritin Concentration: 10mg per ml, coupled with DMPA.
A cationic derivative used to label negatively charged cell membranes for visualization by EM.Danon, D et al. (1972), J. Ultrastruct. Res., 38, 500

0-5ºC

15550

Cationized

1ml

112.00

Fixatives to Fluorescein