Certificates of Analysis

EA to FIRM

EA Counterstain, Gill's Modified Solution

For use with Papanicolaou stain; a general tissue stain. Ready-to-use.

RT	26050-05	EA Counterstain, Gill's Modified	500 ml	52.00	Add to Cart
RT	26050-06	EA Counterstain, Gill's Modified	1 L	95.00	Add to Cart

EARLE's Balanced Salt Solutions - EBSS

All of our Earle's Balanced Salt Solution contains: Potassium Chloride, Sodium Chloride, Sodium Phosphate Monobasic, and D-glucose. Some formulations may contain additional components. Use the table below as a quick reference for identifying the one that best suits your application. All of the solutions are prepared 1X strength.

Ingredients	F.W.	Formula I	Formula II	Formula III
KCl	74.56	X	X	X
NaCl	58.44	X	X	X
NaH2PO4·H2O	137.99	X	X	X
D-Glusose	180-16	1g/L	X	1g/L
MgSO4·7H2O	246.48	X	X	X
CaCl2,·2H2O	147.02	X	X	X
NaHCO3	84.01	X	X	X
Phenol Red		----	0.015g/L	0.050g/L

RT	11765-05	EBSS, Formula I, 0.157M		500 ml	22.00	Add to Cart
RT	11765-10	EBSS, Formula I, 0.157M		1 L	26.00	Add to Cart
RT	11766-05	EBSS, Formula II, 0.152M		500 ml	21.00	Add to Cart
RT	11766-10	EBSS, Formula II, 0.152M		1 L	26.00	Add to Cart
RT	11767-05	EBSS, Formula III, 0.157M		500 ml	21.00	Add to Cart
RT	11767-10	EBSS, Formula III, 0.157M		1 L	26.00	Add to Cart

EDTA, Disodium Salt, Dihydrate, A.C.S.

(Ethylenediamine Tetraacetic acid)
Na₂C₁₀H₁₄O₈·2H₂O   F.W. 372.20   CAS #6381-92-6
A.C.S.

Specifications

Assay.	99+%
Insoluble matter	max 0.005%
Nitrilotriacetic acid	max 0.1%
Heavy Metal (as Pb)	max 0.005%
Iron	max 0.01%

Organic chelating for staining glutaraldehyde fixed tissues, pre-stained with uranyl acetate, followed by lead citrate. Bernard, W., J. Ultrastruct. Res., 27. 250 (1969)

RT

15200

100g

94.00

Add to Cart

EDTA Buffer pH 8.5 (10x)

Description: This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.
Storage: 2-8°C.DO NOT FREEZE
Intended Use:1X buffer solution is intended for heat-induced antigen retriever in IHC. Please refer to primary antibody protocol.
Reagent: 10 X, pH 8.5 antigen retriever solution.
Preparation of Working Solution:Dilute this 10X buffer as needed (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, 1X buffer can be stored at 2-8°C

4-8°C	64142-09	EDTA Buffer pH 8.5	100 ml	75.00	Add to Cart
4-8°C	64142-10	EDTA Buffer pH 8.5	1000 ml	125.00	Add to Cart

Ehrlich's Hematoxylin

Stain selectivity for nuclei. Ready-to-use.

RT	26040-05	Ehrlich's Hematoxylin	500 ml	43.00	Add to Cart
RT	26040-06	Ehrlich's Hematoxylin	1 L	72.00	Add to Cart

EMbed 812

CAS # N.A.
Specific Gravity: 1.08-1.10
EMbed 812 has the same characteristics as Epon 812, physically, and provides the same results in preservation, handling, curing and sectioning. EMbed 812 may cause etching on selected plastics. All formulas for mixing and using Epon 812 can be applied to EMbed 812, e.g. Luft's procedure, Mollenhauer's and EPON-ARALDITE mixtures.

RT	14900		450 ml	28.00	Add to Cart
RT	14901		4x450 ml	106.00	Add to Cart

EMbed 812 Embedding Kit

Please see listing in Embedding Media Kits.

RT	14120	DMP-30 Kit Version	kit	72.00	Add to Cart
RT	14121	BDMA Kit Version	kit	73.00	Add to Cart

EMbed 812 / DER 736 Embedding Kit

Please see listing in Embedding Media Kits.

RT

14130

kit

70.00

Add to Cart

TechnicalTip

An Epon-812 Replacement

Entellan®

Please see listing in Adhesives and Mountants.

RT	14800		100 ml	52.00	Add to Cart
RT	14802		500 ml	196.00	Add to Cart

Eosin Y, Certified, C.N. #DcE-40

(Acid Red 87, Eosin Yellowish)
C_2cH₆Br₄Na₂O₅   F.W. 691.88   CAS #17372-87-1

Solubility: 44.0% Water; 2.0% Alcohol; 25.0% Cellosolve; 27.5% Glycol; 0.0% Xylene.
Used with Hematoxylin for showing cell nuclei in embryos and kidneys. Conn, "Biological Stain" 9th ed., The Williams and Wilkens Co., Baltimore 1977, pg. 7.

Component of Wright's stain for blood corpuscles. Wright, J. Med. Res., 7, 138 (1902)
For staining bone marrow to show cell morphology. Bech, "Lab. Manual of Hematological Tech.", W. B. Saunders Co., Phila., 1908, p. 233

RT	14850		10g	11.00	Add to Cart
RT	14851		25g	24.00	Add to Cart

EPO-Fix Embedding Media

Please see listing in Embedding Media Kits.

Epon-812 Resin

Please see listing in Embedding Media Kits.

Epon-815 Resin;

CAS # NA
Specific Gravity: 1.13
An aromatic resin.

RT

14910

450 ml

27.00

Add to Cart

EMS Cured Epoxy Remover

Studies show that in order to get good results in positive staining of sections, the removal of cured epoxy (embedding material) as well as the reduced-osmium (fixation materials) from the sections is necessary; especially when double fixation with an Aldehyde and Osmium tetroxide is employed to process specimens for E.M. We offer a CURED-EPOXY-REMOVER kit, which removes the polymerized resin as well as the reduced-osmium. The structure of the resin free section will remain unchanged.

RT	14942	Cured Epoxy Remover Kit consists of:		kit	80.00	Add to Cart
		Acetone, Reagent Grade
		Ethyl Alcohol
		Methyl Alcohol
		Picric Acid
		Benzene

TechnicalTip

Re-Embedding of Tissue Poorly Embedded

Epoxy Tissue Stain, Ready-to-use

Toluidine Blue and Basic Fuchsin. For staining semi thin sections of epoxy embedded tissues.
Spurlock, B.O., (1966) et al., Am. J. of Clin. Path.

RT

14950

100 ml

24.00

Add to Cart

Erythrosin B, Certified, C.N. #DcEr-5

(Acid Red 51, Erythrosin extra Bluish; Eosin J; Iodeosin B; Pyrosin B)
C₂₀H₆O₅I₄Na₂   F.W. 879.87   CAS #16423-68-0
Solubility: 10.0% Water; 5.0% Alcohol; 7.0% Cellosolve; 8.25% Glycol; 0.0% Xylene.

RT

15010

10 g

25.00

Add to Cart

ERL 4221-An ERL-4206 Replacement

Due to the supply shortage of ERL-4206 ERL-4221 has now been specially formulated to replace it. ERL 4206 is one of the main components of the low viscosity (Dr. Spur) embedding resin kit.

ERL-4221 is a Cycloaliphatic Epoxide Resin.

Specifications

Specific Gravity (H2O=1):	1.173 @ 20°C/20°C
Flash Point – Closed Cup:	118°C (245°F)
Boiling Point (760mm/Hg):	>250°C (>482°F)

RT	15004	ERL-4221	225 ml	22.00	Add to Cart
RT	15005	ERL-4221	4x225 ml	75.00	Add to Cart

Ethyl Alcohol, Specially Denatured

C₂H₅OH   F.W. 46.07   CAS #64-17-5
Blended 95% Ethanol and 5% Methanol
Suitable for all routine histology/cytology preparations.

Specifications

Evaporation Residue	5ppm max.
Water	0.1%
Filtered SDA-3A	95%

RT	15050		1qt	25.00	Add to Cart
RT	15051		4x1qt	82.00	Add to Cart

Ethyl Alcohol, Anhydrous

(Ethanol) - 200 Proof
CH₃CH₂OH   F.W. 46.07   CAS # 64-17-5

Specifications

Specific Gravity	<0.7942
Purity	>99.9%
Acidity	<0.002%
Water (Karl Fisher)	<0.1%
Non-Volatile Matter	<0.0025%

RT	15055		450 ml	22.00*	Add to Cart
RT	15056		4x450 ml	72.00*	Add to Cart
RT	15058		1 liter	36.00	Add to Cart

*Tax has already been paid

Ethylene Dichloride, Reagent, A.C.S. 1,2-Dichloroethane

Please see 1,2-Dichloroethane.

RT	13250		450 ml	29.00	Add to Cart
RT	13251		4x450 ml	102.00	Add to Cart

Ethylene Glycol Dimethacrylate

(Ethylene dimethacrylate)
C₁₀H₁₄O₄   F.W. 198.22   CAS #97-90-5

Inhibited with 100 ppm HQ. (Hydroquinone)

Specific Gravity:	1.053
b.p.	66-68°C

0-5°C

15300

225 ml

40.00

Add to Cart

Ethylene Glycol Monoethyl Ether, Purified

(Cellosolve; 2-Ethoxyethanol; Ethyl Glycol)
HOCH₂CH₂OCH₂CH₃   F.W. 90.12   CAS #110-80-5

Suitable for histological use.

Specifications

bp	133-135°C
Specific Gravity	@20°C: 0.931
Assay	>99.5%
Water	<0.1%
Residue after evaporation	<0.001%

0-5°C	15310-01		1L	42.00	Add to Cart
0-5°C	15310-06		6x1L	230.00	Add to Cart

EUKITT® Mounting Medium

EUKITT classic has been on the market place since 1953 and has proven to be useful due to its distinct characteristics: Fast Curing, Crystal clear optically with a universal refractive index (1.49). As well, it offers good fluidity, low shrinkage and is easy to use. The EUKITT classic may be used either manually or in an automated mode.

The product holds up well in the presence of water and trace levels of Alcohol. It is very well suited for all pale color preparations as well as colorless preparations.

EUKITT has a neutral pH and offers extended storage times of all slide preparations for years without any sign of cracking or discoloration. The medium itself is colorless and does not change the color and or the structure of the samples mounted with the medium. EUKITT is temperature resistant to heat and cold (-170°C), as well as humidity, daylight and UV light.

Once the EUKITT bottle is opened it is advantageous to seal the bottle tightly as soon as you are done using it. If the medium becomes thickened you can add a small amount of xylene to dilute it.

Specifications

Refractive index n[20°C]	1.49
Viscosity	250-450 mPa*s
Curing time	20 minutes
No yellowing after long storage
No brittle fracture
No self-fluorescence under UV light

RT	15320	EUKITT Mounting Medium	100 ml	70.00	Add to Cart
RT	15322	EUKITT Mounting Medium	500 ml	190.00	Add to Cart

EUKITT® Classic Mounting Medium

The gold-standard of mounting media for cover slipping machines.

EUKITT Classic was formulated for use in Automated Coverslipping machines. Slide preparations with EUKITT Classic exhibit minimal discoloration and perfect drying without any bubbles and streaks. With EUKITT Classic ideal viscosity (500-750 mPa's) it is a perfect formulation for all automated machines.

Supplied in a wide neck, unbreakable aluminium bottle. The product has a shelf life of 3 years.

• Fast drying for rapid results
• Neutral and colorless
• Well proven formulation
• Long-term stability

Specifications

Refractive index n[20°C]	1.49
Viscosity	500-750 mPa*s
Drying time	20 minutes
No yellowing after long storage
No brittle fracture
No self-fluorescence under UV light

RT	15320-10	EUKITT Classic Mounting Medium	100 ml	73.00	Add to Cart
RT	15322-10	EUKITT Classic Mounting Medium	500 ml	215.00	Add to Cart

EUKITT® Neo Xylene Free Mounting Medium

The high-quality classic of mounting media now xylene free.

EUKITT Neo is xylene-free and characterized by fast curing, crystal-clear optics, low fluorescence, favorable refractive index, good fluidity and low shrinkage. The preparations are stable without cracking and discoloration. EUKITT Neo is colorless and does not change shape, structure orcolor of the material to be examined. The unique formulation of this version of EUKITT uses self sustaining raws that have no solvent base at all.

The pleasant odor facilitates working in the laboratory.

Specifications

Refractive index n[20°C]	1.48
Viscosity	250-450 mPa*s
Drying time	20 minutes
No yellowing after long storage
No brittle fracture
No self-fluorescence under UV light

RT	15320-20	EUKITT Neo Mounting Medium	100 ml	72.00	Add to Cart
RT	15322-20	EUKITT Neo Mounting Medium	500 ml	205.00	Add to Cart

EUKITT® Neo Special Mounting Medium

Highly viscous xylene free mounting medium.

• Faster drying for rapid results
• Higher viscosity than EUKITT Neo
• Neutral and colorless
• Long-term stability

EUKITT Neo Special is another xylene free product. EUKITT Neo Special is characterized by faster curing and slightly higher viscosity than EUKITT Neo. Users of EUKITT Classic will appreciate the xylene free replacement. The product retains crystal-clear optics, low fluorescence, favorable refractive index, good fluidity and low shrinkage properties. The preparations are stable without cracking and discoloration.

EUKITT Neo Special is colorless and does not change shape, structure or color of the material to be examined. The use of sustainable, renewable raw material as a solvent completes the modern formulation approach.

The pleasant odor facilitates working in the laboratory. Supplied in wide neck, unbreakable aluminum bottles. The product has a shelf life of 3 years.

Specifications

Refractive index n[20°C]	1.48
Viscosity	500-750 mPa*s
Drying time	15 minutes
No yellowing after long storage
No brittle fracture
No self-fluorescence under UV light

RT	15320-25	EUKITT Neo Special Mounting Medium	100 ml	75.00	Add to Cart
RT	15322-25	EUKITT Neo Special Mounting Medium	500 ml	218.00	Add to Cart

EUKITT® UV Mounting Medium

EUKITT UV is the first mounting media of its kind to eliminate the need to be concerned about the vaport pressure of the solvent used.

EUKITT UV is a UV-curing mounting medium with all the well-known beneficial properties of the gold standard series EUKITT and a true innovation in the world of mounting. EUKITT UV does not contain any solvents, therefore only 50% of the usual amount is needed compared to conventional systems. While using EUKITT UV almost no shrinkage can be detected.

The mounting medium may be used on tissue sections in non-polar solvents such as xylene or xylene substitutes as well as directly on sections in pure alcohols (EtOH, iPrOH, etc.).

The viscosity of EUKITT UV is very user-friendly. After being applied to the tissue, the cover glass can be placed as usual. Subsequently, a transfer break of at least 120 sec. is maintained prior to irradiation in order to ensure an ideal penetration into the tissue.

When using the specially tuned LED CCFL UV curing machine EUKITT UV cures in just 30 seconds.

Specifications

Refractive index n[20°C]	1.48
Viscosity	350-550 mPa*s
Curing time	10-30 seconds
No yellowing after long storage
No brittle fracture
Suitable for all tissue types
Shelf life of a 100-ml bottle at room temperature: 6 months

RT

15320-30

EUKITT UV Mounting Medium

100 ml

88.00

Add to Cart

EUKITT® Low-Viscosity UV-R Mounting Medium

EUKITT UV-R has a lower viscosity that EUKITT UV, is fast spreading and compatible with almost all clearing agents like xylene, iso-paraffine, petroleum ether, limonenes, etc. In addition, it can be directly applied on sections from absolute EtOH or I-PrOH.

The ability to remove UV-R under classic conditions (24 hours xylene) is appealing for many applications. This product can be used manually or in cover slipping machines.

Specifications

Refractive index n[20°C]	1.48
Curing time (UV light, 365nm, 1 watt)	60-120 seconds
No yellowing after long storage
No brittle fracture
Suitable for all tissue types
Shelf life of a 100-ml bottle at room temperature: 12 months

RT

15320-30R

EUKITT Low-Viscosity UV-R Mounting Medium

100 ml

88.00

Add to Cart

EUKITT® Microfix™ Mounting Medium

MICROFIX™ follows the proven, xylene-based approach of O. Kindler ORSAtec. MICROFIX™ is characterized by fast curing, crystal-clear optics, low fluorescence, ideal refractive index, good flowability and low shrinkage. MICROFIX™ is colorless and does not change shape, structure or color of the material to be examined. These characteristics are already known from the classic EUKITT. What is new is the streamlined and optimized synthesis and filling process, and a still further optimized selection of our raw materials.

Specifications

Refractive index n[20°C]	1.49
Viscosity	250-450 mPa*s
Drying time	20 minutes
No yellowing after long storage
No brittle fracture
No primary fluorescence under UV light

RT	15320-35	EUKITT Microfix™ Mounting Medium	100 ml	54.00	Add to Cart
RT	15322-35	EUKITT Microfix™ Mounting Medium	500 ml	149.00	Add to Cart

EUKITT® EMS 4400 LB

EUKITT EMS 4400 LB is a single-component resin that polymerizes under UV light. It is used for embedding and preparing of thin ground sections. Thin ground sections for medical and technical applications can be produced. It is the latest embedding medium, and a worldwide innovation, due to its unique properties.

Properties of EUKITT EMS 4400 LB

EUKITT EMS 4400 LB is specially developed for thin ground sectioning technology. It completely passes through hard and soft tissue. Due to its mechanic strength, it is extremely suitable for the thin sectioning technique.

The hardening of the embedding medium is done by UV light of a wavelength of 365 nm with 2 x 9 W. The exposure takes place from two sides. During the exposure within the given time (see instruction manual/operating instructions) temperatures of 50°C will not be exceeded.

Fast staining is possible with all known methods, and with the same high quality.

EUKITT EMS 4400 LB does not contain monomers, therefore the vapour pressure is very low. As a consequence the user is not bothered by intense, toxic smell. EUKITT EMS 4400 LB is characterized by crystal-clear optics with a favorable refractive index, good fluidity and low shrinkage.

Fixation

Every common fixation for light microscopy is possible for this outstanding embedding system.

Drainage/Infiltration

The drainage and infiltration of the tissue is possible via an ascending range of alcohol/EUKITT EMS 4400 LB (for this, see theinstruction manual).

Embedding and polymerization

Embedding with EUKITT EMS 4400 LB can be performed with standard techniques. Take care when filling the embedding mold, so that no air bubbles are enclosed.

Polymerization (hardening) is done by exposure through a lamp system of 365 nm and 2 x 9 W to comply with the steps in the instruction manual The entire polymerization takes a maximum of 4 hours.

EUKITT EMS 4400 LB is available in 500 ml units only.

RT

15320-40

EUKITT EMS 4400 LB

500 ml

225.00

Add to Cart

Farnesol

(3,7,11-Trimethyl-2,6,10-dodecatrien-1-ol)
C₁₅H₂₆O   F.W. 222.57   CAS #4602-84-0
Specific Gravity: 0.886
Mixture of Stereoisomers: Approx. 90% Synthetic

0-5°C

15340

10 ml

24.00

Add to Cart

Fast Green FCF, Certified, C.N. #DcGf-12

(Food Green 3)
C₃₇H₃₄O₁₀N₂S₃Na₂   F.W.808.86   CAS #2353-45-9

Solubility:

4.0% Water; 9.0% Alcohol; 8.0% Cellosolve; 6.25% Glycol; 0.0% Xylene

The main advantage of this dye is its resistance to fading. Mammalian tissue stain for visualizing collagen, muscle, cystoplasma, cells and cornified epithelium. Kornhauser, Stain Tech., 18,95 (1943); 20, 23 (1945); Lillie, Arch. Path., 29, 705 (1940). Modified Feulgen stain for visualizing chromosomes in root tips and other meristematic tissue. DeTomasi, Stain Tech., 11, 137 (1936). Used in plant histology. O. Conn, "Biological Stains" 9th ed., Williams & Wilkens Co., Baltimore, 1977, pp. 252-3; Haynes, Stain Tech., 3, 40 (1928).

RT

15500

5g

24.00

Add to Cart

Fast Green Solutions

Please see listing in Prepared Staining Solutions.

Ferric Chloride, Hexahydrate, Reagent, A.C.S.

FeCl₃·6H₂O   F.W. 270.30   CAS #13478-10-9

Assay	97.0-102%
Insoluble Matters.	0.01%
Nitrate (about 0.001%)	To Pass Test
Phosphorous Compound	0.01%
Sulfate	0.01%
Arsenic	0.002%
Copper	0.003%
Zinc	0.003%

References for use: Membrane stains

• Gasic, G., Berwick et al (1963). Hale stain for sialic acid containing mucins. J. Cell Biol., 19:223
• Gasic, G., Berwick, et al (1968). Positive and Negative colloidal iron as cell surface electron stains. Lab. Invest., 18:63
• Blanquet, P.R. and Loiez, A. (1974). Colloidal iron used at pHs lower than 1 as electron stain for surface proteins. J. Histochem. Cytochem., 22:368
• Matukafs, V.J., Panner et al (1967). Studies on ultrastructural identification and distribution of protein-polysaccharides in cartilage matrix. J. Cell Biol., 32:365
• Benedetti, E.L. and Emmelot, P. (1967). Studies on plasma membranes IV. The Ultrastructural localization and content of sialic acid in plasma membranes isolated from rat liver and hepatoma. J. Cell Sci., 2:499
• Rowley, J.R. (1971). Resolution of channels in the exine by translocation of colloidal iron. Pro. 29th Ann. EMSA Meet., p.352. Claitor's Pub. Division, Baton Rouge, LA
• Nicolson, G.L. (1973). Anionic sites of human erythrocyte membranes.I. Effects of trypsin, phospholipase C, and pH on the topography of bound positively charged colloidal particles. J. Cell Biol., 57:373
• Hendy, R. (1971). Electron microscopy of lipofucsin pigment stained by the Schmorl and Fontana technique. Histochemie, 26:311.

RT

15510

Ferric Chloride, Hexahydrate, Reagent

500g

45.00

Add to Cart

Ferric Chloride Solutions

Please see listing in Reagents for Histology.

Ferritin Cationized

CAS #9007-73-2

This polycationic derivative of ferritin is useful in labeling negative charges on cell surfaces and to study membrane structure. Cationized ferritin is prepared by coupling ferritin from horse spleen with N,N-dimethyl-1,3-propanediamine (DMPA). Sterile filtered solution in 0.15M NaCl.
Ferritin Concentration: 10mg per ml, coupled with DMPA.
A cationic derivative used to label negatively charged cell membranes for visualization by EM.Danon, D et al. (1972), J. Ultrastruct. Res., 38, 500

0-5°C

15550

Cationized

1ml

162.00

Add to Cart

Fluorophore-Infiltrated Resin Microscopy (FIRM)

Rapid staining technique that provides high-contrast fluorescence microscopy imaging of resin embedded samples, reminiscint of low magnification transmission electron microscopy.

• Works with Epoxy and Acrylic type resins
• Simply mount your sections on glass slides, air dry, and overlay with 100 microliters FIRM for 30 seconds, wash and coverslip with water or an aqueous mountant. View in Rhodamine channel of a standard wide field Fluorescence Microscope.
• FIRM staining is unique in that the brightly fluorescent dye infiltrates the resin, revealing tissue structures in negative relief, with extremely high contrast and resolution.
• Perfect for previewing specimens prior to EM Analysis
• High Throughput, high contrast image phenotyping of model organisms such as drosophila
• Ideal for material and food science analytical microscopy

RT	12780-05	FIRM for Acrylic Resin Sections		10ml	95.00	Add to Cart
RT	12780-06	FIRM for Epoxy Resin Sections		10ml	95.00	Add to Cart

Certificates of Analysis

Fixatives to Fluorescein