Chemicals for Microscopy

Fixatives, Prepared to Fluorescein

Premixed, prebuffered fixatives, that are pH adjusted for immediate laboratory use. They are freshly prepared upon receipt of order to ensure maximum shelf life. The fixatives are filled in 20 ml glass vials (10 ml/vial). This allows for the immediate fixation of your specimens without costly and time consuming transfer associated with ampoule contained fixatives.

*If you desire any prepared fixative that is not already listed in our catalog or you have special size and packaging requirements, please call us.

0-5°C	15953	0.2% Formaldehyde/0.1% Chromic Acid in 0.1M Sodium Cacodylate Buffer, pH 7.4	10x10 ml	32.00	Add to Cart
0-5°C	15956	2% Formaldehyde/0.02% Picric Acid in 0.1M Sodium Phosphate Buffer, pH 7.2	10x10 ml	32.00	Add to Cart
0-5°C	15949	Formaldehyde/Glutaraldehyde, 2.5% each in 0.1M Sodium Cacodylate Buffer, pH 7.4	10x10 ml	33.00	Add to Cart
0-5°C	15950	Formaldehyde/Glutaraldehyde, 3% each in 0.1M Sodium Cacodylate Buffer, pH 7.4	10x10 ml	33.00	Add to Cart
0-5°C	15960	2.5% Glutaraldehyde in 0.1M Sodium Cacodylate Buffer, pH 7.4	10x10 ml	31.00	Add to Cart
0-5°C	15970	2.5% Glutaraldehyde in 0.1M Millonig's Sodium Phosphate Buffer pH 7.2	10x10 ml	30.00	Add to Cart
0-5°C	15980	2.5% Glutaraldehyde in 0.1M Sorensen's Sodium- Potassium Phosphate Buffer, pH 7.2	10x10 ml	30.00	Add to Cart

0.2% Formaldehyde/0.1% Chromic Acid	15953
2% Formaldehyde/0.02% Picric Acid i	15956
Formaldehyde/Glutaraldehyde, 2.5%	15949
Formaldehyde/Glutaraldehyde, 3%	15950
2.5% Glutaraldehyde	15960

Bouin's Fixative

A fixative that is used in light microscopy for the preparation of delicate structure.

15990

10x10 ml

30.00

Add to Cart

Hollande’s Fixative

Please see Hollande’s Fixative

Karnovsky's Fixative:

Please see Paraformaldehyde-Glutaraldehyde Solution Karnovsky's Fixative.

0-5°C	15720		kit	18.00	Add to Cart
0-5°C	15730		box of 5 kits	80.00	Add to Cart

Trump's Fixative

A combination of Sodium Cacodylate, Formalin, and Glutaraldehyde. Ready to use.

0-5°C	11750		500 ml	39.00	Add to Cart
0-5°C	11752		4x500 ml	142.00	Add to Cart

Paraformaldehyde, Prepared Fixatives

In addition to our complete line of Formaldehyde solutions we have now added to the line.

Paraformaldehyde 0.5% in 0.1M phosphate buffer pH7.4

RT	15951-10	Paraformaldehyde, 0.5% each in 0.1M Phosphate Buffer	10x10 ml	31.00	Add to Cart
RT	15951-15	Paraformaldehyde, 0.5% each in 0.1M Phosphate Buffer	500 ml	96.00	Add to Cart

Paraformaldehyde 1% Aqueous

RT	15953-10	Paraformaldehyde, 1% each Aqueous	10x10 ml	19.00	Add to Cart
RT	15953-15	Paraformaldehyde, 1% each Aqueous	500 ml	88.00	Add to Cart

Paraformaldehyde 1% in 0.1M Phosphate Buffer pH, 7.4

RT	15952-10	Paraformaldehyde, 1.0% each in 0.1M Phosphate Buffer	10x10 ml	32.00	Add to Cart
RT	15952-15	Paraformaldehyde, 1.0% each in 0.1M Phosphate Buffer	500 ml	96.00	Add to Cart

Paraformaldehyde 1% Glut 2% in 0.1M Phosphate buffer, pH 7.4

RT	15949-30	Paraformaldehyde 1 % Glutaraldehyde 2% in 0.1M Phosphate Buffer	10x10 ml	33.00	Add to Cart
RT	15949-35	Paraformaldehyde 1 % Glutaraldehyde 2% in 0.1M Phosphate Buffer	500 ml	99.00	Add to Cart

Paraformaldehyde 3% Glutaraldehyde 0.35% in 0.1M Sodium Cacodylate, pH 7.4

RT	15949-50	Paraformaldehyde 3 % Glutaraldehyde 0.35% in 0.1M Sodium Cacodylate Buffer	10x10 ml	37.00	Add to Cart
RT	15949-60	Paraformaldehyde 3 % Glutaraldehyde 0.35% in 0.1M Sodium Cacodylate Buffer	500 ml	104.00	Add to Cart

Paraformaldehyde 4% and Glutaraldehyde 1% in Phosphate Buffer, pH 7.0

RT	15949-70	Paraformaldehyde 4 % Glutaraldehyde 1% in 0.1M Phosphate Buffer	10x10 ml	39.00	Add to Cart
RT	15949-80	Paraformaldehyde 4 % Glutaraldehyde 1% in 0.1M Phosphate Buffer	500 ml	106.00	Add to Cart

Paraformaldehyde 4% in 0.1M Sodium Cacodylate, pH7.4

RT	15952-10S	Paraformaldehyde 4 % in 0.1M Sodium Cacodylate Buffer	10x10 ml	42.00	Add to Cart
RT	15952-15S	Paraformaldehyde 4 % in 0.1M Sodium Cacodylate Buffer	500 ml	122.00	Add to Cart

Paraformaldehyde 4% in 0 .1M Phosphate Buffer, pH 7.4

RT	15735-10S	Paraformaldehyde 4% in 0 .1M Phosphate Buffer	1L	32.00	Add to Cart
RT	15735-20S	Paraformaldehyde 4% in 0 .1M Phosphate Buffer	4L	98.00	Add to Cart

Paraformaldehyde 4% in 0 .2M Phosphate Buffer, pH 7.4

RT	15735-30S	Paraformaldehyde 4% in 0 .2M Phosphate Buffer	1L	32.00	Add to Cart
RT	15735-40S	Paraformaldehyde 4% in 0 .2M Phosphate Buffer	4L	98.00	Add to Cart

Paraformaldehyde 4% in 0 .1M Phosphate Buffer, pH 7.2

RT	15735-50S	Paraformaldehyde 4% in 0 .1M Phosphate Buffer	1L	36.00	Add to Cart
RT	15735-60S	Paraformaldehyde 4% in 0 .1M Phosphate Buffer	4L	100.00	Add to Cart

Paraformaldehyde 4% in 0 .2M Phosphate Buffer, pH 7.2

RT	15735-70	Paraformaldehyde 4% in 0 .2M Phosphate Buffer	10x10 ml	36.00	Add to Cart
RT	15735-80	Paraformaldehyde 4% in 0 .2M Phosphate Buffer	500 ml	100.00	Add to Cart

Paraformaldehyde 4% in 0 .1M Phosphate

BufferSaline, pH7.4

RT	15735-85	Paraformaldehyde 4% in 0 .1M Phosphate Buffer Saline	10x10 ml	38.00	Add to Cart
RT	15735-90	Paraformaldehyde 4% in 0 .1M Phosphate Buffer Saline	500 ml	105.00	Add to Cart

Paraformaldehyde 4% in 0 .2M Phosphate Buffer Saline, pH 7.4

RT	15735-95	Paraformaldehyde 4% in 0 .2M Phosphate Buffer	10x10 ml	38.00	Add to Cart
RT	15735-100	Paraformaldehyde 4% in 0 .2M Phosphate Buffer	500 ml	100.00	Add to Cart

B-5 Fixative

For lymph nodes.

RT	64122-05	B-5 Fixative	500 ml	64.00	Add to Cart
RT	64122-06	B-5 Fixative	1 lt	120.00	Add to Cart
RT	64122-10	B-5 Fixative	4 lt	450.00	Add to Cart

Bouin’s Solution

Bouin’s solution can be used as a fixation and a staining fluid. Bouin’s fixative is excellent for use on biopsy specimens of the gastrointestinal tract. Tissue from the endocrine system are well fixed and many antibodies react well with tissue fixed in Bouin’s. Bouin’s solution may be used as a routine fixative or a staining solution, but should not be used as a preservative for tissue. Tissue cannot be held in Bouin’s solution for extended periods. The maximum fixation time in this solution should be less than 48 hours and should be transferred to alcohol. Packaged in leak-proof histo-containers, prefilled and ready-to-use.

Formulated with Picric Acid saturated aqueous solution 75%, Formalin (40% aqueous formaldehyde) 25% and Glacial Acetic acid 5% in de-ionized water

RT	15990-01	Bouin's Fluid	1 L	42.00	Add to Cart
RT	15990-04	Bouin's Fluid	4 L	146.00	Add to Cart

Bouin’s Fluid in Pre-filled cups

RT	15990-10	Bouin's Fluid	50 x 10 ml/cs	76.00	Add to Cart
RT	15990-20	Bouin's Fluid	50 x 20 ml/cs	96.00	Add to Cart
RT	15990-30	Bouin's Fluid	50 x 30 ml/cs	43.00	Add to Cart

Brasil Fixative

Ethanol, Picric Acid, Formalin, Acetic Acid, Glacial
This fixative is most popular when glycogen is the target. The solution is similar to Bouins Solution (alcoholic) offering quick hardening and good staining with acid dyes.

RT	15995-10	Brasil Fixative	10x10 ml	38.00	Add to Cart
RT	15995-20	Brasil Fixative	100 ml	36.00	Add to Cart

Carnoy’s Fluid

Stock solution.

RT	64130-05	Carnoy’s Fluid	500 ml	26.00	Add to Cart
RT	64130-50	Carnoy’s Fluid Without Chloroform	500 ml	24.00	Add to Cart

Carson’s Phosphate Buffered Formalin

Millonig’s phosphate buffered formalin as modified by Carson, Martin and Lynn, by using 4% formalin in 0.1M sodium phosphate monobasic with sodium hydroxide to pH 7.2

RT	15732-05	Carson’s Phosphate Buffered Formalin	500 ml	15.00	Add to Cart
RT	15732-10S	Carson’s Phosphate Buffered Formalin	1 L	28.00	Add to Cart

For some tissues, the use of formalin (contains 10 – 15% Methanol) is not suitable, we offer the alternative formaldehyde (prepared from paraformaldehyde – contains no methanol) for your convenience.

RT	15733-05	Carson’s Phosphate Buffered Paraformaldehyde	500 ml	18.00	Add to Cart
RT	15733-10	Carson’s Phosphate Buffered Paraformaldehyde	1 L	32.00	Add to Cart

CytoPrep Fixative

CytoPrep Fixative forms a clear protective coating over specimens while fixing cells. It is ideal for cytological smears and it dries within 4 to 6 minutes. Good for safe storage and transportation of slides. It is water soluble and contains no chlorofluorocarbons. Comes in a 4 oz. spray can.

RT	64145-01	CytoPrep Fixative	4 oz	16.00	Add to Cart
RT	64145-12	CytoPrep Fixative	12x4 oz	175.00	Add to Cart

Davidson’s Fixative (Hartmann’s/ Davidson’s Fixative)

his is rapid fixative. Results with good nuclear detail and minimal formalin pigment. Hatmann’s (Davidson’s) Fixative is useful for preparing tumors, bone marrow, gynecologic specimen, fatty breast, medical biopsies. Fixation of small specimen is rapid. Exposure to the fixative should be limited to 24 hours (tissue may then be transferred to neutral buffered formalin or 70% alcohol for storage).

Based on the volume of 1125 ml solution, there is 2 part Formalin (35-39%), 3 part Ethanol, 1 part Glacial Acetic acid, and 3 part of tap water.

RT	64133-10	Hartmanns (Davidsons Fixative)	1 L	76.00	Add to Cart
RT	64133-14	Hartmanns (Davidsons Fixative)	4 L	213.00	Add to Cart

Modified Davidson’s Fixative

(14% Ethyl Alcohol, Denatured, 37.5% Formalin 37-39%, 6.25% Glacial Acetic Acid, and D.I. Water)

64133-50

Modified Davidson's Fixative

38.00

Add to Cart

Dietrich’s Fixative

Ethanol, formalin, glacial acetic acid, distilled water

RT	15958-10	Dietrich’s Fixative	10x10 ml	39.00	Add to Cart
RT	15958-20	Dietrich’s Fixative	100 ml	37.00	Add to Cart

Fast Frozen Fix

Prepared fixative – This is one step quick fix for frozen sectioned tissue. Fixes tissue to slide to allow for staining procedures. Apply to most tissue types.

RT	15280-01	Fast Frozen Fix™	100 ml	75.00	Add to Cart
RT	15280-05	Fast Frozen Fix™	500 ml	130.00	Add to Cart

Fixative/Decalcifier

A solution that fixes and decalcifies in one step.
Prevents loss of cellular structure.
Enhances hematoxylin staining.

RT	64148-01	Fixative/Decalcifier	1 L	44.00	Add to Cart
RT	64148-06	Fixative/Decalcifier	6x1 L	240.00	Add to Cart

Formalde-Fresh Low Odor Solution; Histology Grade

A specially formulated odor-mask which neutralizes the formalin odor without affecting subsequent tissue processing, handling, or staining. Low odor, ready-to-use 10% formalin solution. May be used as a fixative or preservative of specimens. Its shelf life is 24 months.

RT	15678-01	Formalde-Fresh	4 L	79.00	Add to Cart
RT	15678-04	Formalde-Fresh	4x4 L	315.00	Add to Cart

Buffered Formalde-Fresh, Low-Odor 10% Formalin Solution

Low odor, prepared w/v, pH 6.9 to 7.1 at 25°C. 10% formalin in Phosphate buffer according to the A.F.I.P. Manual of Histology. This solution allows for long-term storage of tissue specimens.

RT	15741-01	Low-Odor Formalin 10% Buffered	4 L	58.00	Add to Cart
RT	15741-04	Low-Odor Formalin 10% Buffered	4x4 L	200.00	Add to Cart

Formalin, Neutral, Buffered 10% w/v in Acetate Buffer

Buffered with Sodium Acetate in accordance with the A.F.I.P. manual of Histology. Filtered and ready-to-use.
pH 6.0 to ph 7.1 at 20°C

RT	15738-01	Formalin 10% Buffered in Acetate	1L	18.00	Add to Cart
RT	15738-04	Formalin 10% Buffered in Acetate	4L	49.00	Add to Cart

Formalin, Neutral, Buffered 10% w/v in Phosphate Buffer

Buffered with phosphate in accordance with the A.f.I.P. manual of Histology.Filtered and ready-to-use.
pH 6.9 to 7.1 at 25°C

RT	15740	Formalin 10% Buffered in Phosphate	450 ml	12.00	Add to Cart
RT	15740-01	Formalin 10% Buffered in Phosphate	1L	19.00	Add to Cart
RT	15740-04	Formalin 10% Buffered in Phosphate	4L	56.00	Add to cart

TissuePrep Buffered 10% Formalin

Prefilled with 10% formalin Cat# 15740. Ready-to-use in pre-filled cups. Labeled in accordance to OSHA requirements, which gives you immediate information regarding handling and first aid procedures. Pre-measured in wide-mouth, leak-proof, opaque polypropylene containers which limits your exposure to formaldehyde and saves you time.

	Catalog #	Cup Size	Filled	Pack/cs	Price
RT	15742-10	15 ml	10 ml	50	77.00	Add to Cart
RT	15742-20	30 ml	20 ml	50	98.00	Add to Cart
RT	15742-30	60 ml	30 ml	20	44.00	Add to Cart
RT	15742-60	120 ml	60 ml	20	48.00	Add to Cart

Formalin Prefilled HistoTainer™

The HistoTainer™ (Empty Vials, see page 482) is half filled with 10% Neutral Buffered Formalin as a fixative. These ready-to-use prefilled containers are timer saving and make your work more efficient.

	EMS #	Description	Vial Size	Prefilled	Qty
RT	15743-20	Formalin Prefilled HistoTainer	20 ml	10 ml	24	26.00	Add to Cart
RT	15743-40	Formalin Prefilled HistoTainer	40 ml	20 ml	24	30.00	Add to Cart
RT	15743-60	Formalin Prefilled HistoTainer	60 ml	30 ml	24	36.00	Add to Cart
RT	15743-90	Formalin Prefilled HistoTainer	90 ml	45 ml	24	42.00	Add to Cart
RT	15743-120	Formalin Prefilled HistoTainer	120 ml	60 ml	24	43.00	Add to Cart

Formalin 1% Non Buffered

RT	15734-01	Formalin 1% Non-Buffered	1L	16.00	Add to Cart
RT	15734-04	Formalin 1% Non-Buffered	4L	48.00	Add to Cart

Formalin 2%, Non-Buffered

RT	15735-01	Formalin 2% Non-Buffered	1L	16.00	Add to Cart
RT	15735-04	Formalin 2% Non-Buffered	4L	48.00	Add to Cart

Formalin 3%, Non-Buffered

RT	15735-10	Formalin 3% Non-Buffered	1L	17.00	Add to Cart
RT	15735-20	Formalin 3% Non-Buffered	4L	49.00	Add to Cart

Formalin 4%, Non-Buffered

RT	15735-30	Formalin 4% Non-Buffered	1L	17.00	Add to Cart
RT	15735-40	Formalin 4% Non-Buffered	4L	49.00	Add to Cart

Formalin 5%, Non-Buffered

RT	15735-50	Formalin 5% Non-Buffered	1L	18.00	Add to Cart
RT	15735-60	Formalin 5% Non-Buffered	4L	49.00	Add to Cart

Formaldehyde to Gill's

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Electron Microscopy Sciences specializes in the manufacturing, preparation and distribution of the highest quality laboratory chemicals and microscopy supplies and equipment for electron microscopy, light microscopy, and histology.