Electron Microscopy Sciences

               
View Cart || Request a Catalog

Chemicals for Microscopy

Electron Microscopy Sciences

arrow14 Formaldehyde (Formalin) to Gill's

ormaldehyde, Reagent, arrow12Formaldehyde, Reagent, A.C.S. (Formalin)

HCHO   F.W. 30.03  CAS #50-00-0
Specific Gravity: 1.09
A.C.S. Specifications:

Assay   35.5 to 38.0%
Residue after ignition   0.005%
Color (A.P.H.A.)   10
Sulfate (SO4)   0.002%
Heavy Metal (as Pb)   5ppm
Titrate Acid   0.006 meq/g
Chloride (Cl)   5ppm
Iron (Fe)   5ppm
RT 15680   450ml 12.00 cart
RT 15681   4x450ml 40.00 cart
RT 15682   1qt(946ml) 22.00 cart

arrow12Formaldehyde, 37%, Microfiltered (Formalin Microfiltered)

HCHO   F.W. 30.03   CAS# 50-00-0
Microfiltered with a 0.45 micron membrane; suitable as an EM fixative when a methanol free solution is not necessary.

RT 15686   10x10ml 25.00 cart

msdsarrow12Formaldehyde

(Paraformaldehyde) Glutaraldehyde, Karnovsky's Fixative Kit:
Please see Paraformaldehyde-Glutaraldehyde Solution Karnovsky's Fixative.

  15720   kit 17.00 cart
  15730   box of 5 kits 76.50 cart

Formaldehyde Solutionarrow12Formaldehyde Aqueous Solution (Paraformaldehyde Aqueous Solution), EM Grade

Paraformaldehyde - methanol free solution. A more efficient and rapid penetrant fixative used in combination with Glutaraldehyde and Acrolein fixatives. Easy to break, prescored, 10ml ampoules sealed under inert gas, or

16% Paraformaldehyde 15700 msds
10% Paraformaldehyde 15712 msds
20% Paraformaldehyde 15713 msds
32% Paraformaldehyde 15714 msds

100ml bottles.

RT 15700 16% Paraformaldehyde (formaldehyde) aqueous solution 10ml 5.00 cart
RT 15710 16% Paraformaldehyde (formaldehyde) aqueous solution 10x10ml 22.50 cart
RT 15710-S 16% Paraformaldehyde (formaldehyde) aqueous solution 100ml bottle 21.00 cart
RT 15711 16% Paraformaldehyde (formaldehyde) aqueous solution 10x10ml 210.00 cart
RT 15712 10% Paraformaldehyde (formaldehyde) aqueous solution 10x10ml 20.50 cart
RT 15712-S 10% Paraformaldehyde (formaldehyde) aqueous solution 100ml 19.00 cart
RT 15713 20% Paraformaldehyde (formaldehyde) aqueous solution 10x10ml 26.00 cart
RT 15713-S 20% Paraformaldehyde (formaldehyde) aqueous solution 100ml 24.00 cart
RT 15714 32% Paraformaldehyde (formaldehyde) aqueous Solution 10x10ml 31.00 cart
RT 15714-S 32% Paraformaldehyde (formaldehyde) aqueous solution 100ml 29.00 cart

msdsIn addition to our complete line of Formaldehyde solutions shown above we have now added to the line.

Since the DOT (Department of Transportation) has changed the definition of what a non dangerous solution of formaldehyde is to under 10%, we are now offering an 8% and a 4% solution. This will allow us to ship overnight as well as overseas without any hazardous charges saving our customers a good deal of money.

The quality of our 8% and 4% aqueous Paraformaldehyde solution is as good as all of the other concentrations that we offer.

RT 157-4 4% Paraformaldehyde (formaldehyde) aqueous solution 10x10ml 18.00 cart
RT 157-4-100 4% Paraformaldehyde (formaldehyde) aqueous solution 100ml 17.00 cart
RT 157-8 8% Paraformaldehyde (formaldehyde) aqueous solution 10x10ml 19.50 cart
RT 157-8-100 8% Paraformaldehyde (formaldehyde) aqueous solution 100ml 18.00 cart

msdsarrow12Perfusion Wash and Fixative Ready to Use

For all of the best results we offer ready to use Perfusion washes and fixatives which are recommended by the NeuroScience Association. All of our solutions are ready made and are of the highest quality.

We have three groups of solutions commercially available.

Group I :

Perfusion wash and Fixative Technical Grade. To be used for Standard stains:

Perfusion Wash (1220SK): Phosphate Buffered Saline 01.M
Phosphate Buffer pH 7.2-7.4

Perfusion Fixative(1221SK): 10% Phosphate Buffered
Formaldehyde

Group II:

Perfusion wash and Fixative Reagent Grade. To be used for Standard and ImmunoHistochemistry Stains.

Perfusion Wash (1219SK): Phosphate Buffered Saline 0.1M
Phosphate Buffer pH 7.2-7.4

Perfusion Fixative (1224SK): Paraformaldehye 4%/Sucrose
4% in Phosphate Buffer 0.2M Ph 7.2

Group III:

Perfusion wash and Fixative Superior Reagent Grade.

For the best results use this group. This is Sodium cacodylate based for NSA’S Disintegrative Degeneration Stain

Perfusion Wash (1222SK): 0.8% NaCl/0.4% Dextrose/0.8% Sucrose/ 0.023% CaCl2 /Sodium

Cacodylate 0.034%

Perfusion Fixative (1223SK): 4% Paraformaldehyde/4% Sucrose/ 1.4% Sodium Cacodylate

For a complete procedure and protocol for the use of these washes and fixatives please see the following site: www.neuroscienceassociates.com/perf-solution1 or 2 or 3.htm

  1220SK Perfusion Wash, Technical 1000 ml 24.00 cart
  1221SK Perfusion Fixative, Technical 1000 ml 38.00 cart
  1219SK Perfusion Wash, Reagent 1000 ml 25.00 cart
  1224SK Perfusion Fixative, Reagent 1000 ml 40.00 cart
  1222SK Perfusion Wash, Super Reagent 1000 ml 26.00 cart
  1223SK Perfusion Fixative, Super reagent 1000 ml 42.00 cart

If you want a custom wash or fixative made please just let us know and we will be happy to make you whatever you require

Technical Tip:

The Fixative for Use in Fixing Cornea

Ampoule Breaker; Soft Silicon arrow12Ampoule Breaker; Soft Silicon

Reusable – Made from soft silicone. It offers a simple way to break pre-scored glass ampoules safely. Accommodates 5, 7, and 10 ml size ampoules. Reduces injury and spillage.

60600 Ampoule Breaker each 15.00 cart

Ampoule Crackerarrow12Ampoule Cracker™

The Ampoule Cracker™ is a disposable plastic fracture and safety collar, which is specially designed to aid in the breaking open of pre-scored glass ampoules. Simply slip the Ampoule Cracker™ over the top at the pre-scored portion, snap it, and discard both the ampoule top and the safety collar to prevent cross contamination

60605-04 Ampoule Cracker for Ampoule Size 1 – 4 ml 500/pk 35.00 cart
60605-10 Ampoule Cracker for Ampoule Size 5 – 10 ml 500/pk 35.00 cart
60605-20 Ampoule Cracker for Ampoule Size 10 – 20 ml 500/pk 67.00 cart

msdsarrow12Formalin Spill Control; F-gard- ER

For the handling and disposing of formaldehyde containing solutions. It immobilizes, cools, and contains spills. As well, it reduces the escape of formaldehyde gas and significantly reduces formalin concentration. F-gard- ER aids in reducing exposure to formaldehyde gases by immobilizing the formalin and transforming it to an easily removed semi-solid mass. The mass cools rapidly minimizing formaldehyde off-gassing.

  24140-01 Formalin Spill Control 11 oz 16.00 cart
  24140-06 Formalin Spill Control 6 x 11 oz 90.00 cart

arrow12Formalin, Low odor

Please see listing in Histology: Chemicals for Fixing and Dehydrating.

msdsarrow12Formalin, Neutral, Buffered 10% w/v in Acetate Buffer

Buffered with Sodium Acetate in accordance with the A.F.I.P. manual of Histology. Filtered and ready-to-use.
pH 6.0 to ph 7.1 at 20°C

RT 15738-01 Formalin 10% Buffered in Acetate 1L 18.00 cart
RT 15738-04 Formalin 10% Buffered in Acetate 4L 49.00 cart

msdsarrow12Formalin, Neutral, Buffered 10% w/v in Phosphate Buffer

Buffered with phosphate in accordance with the A.f.I.P. manual of Histology.Filtered and ready-to-use.
pH 6.9 to 7.1 at 25°C

RT 15740 Formalin 10% Buffered in Phosphate 450ml 12.00 cart
RT 15740-01 Formalin 10% Buffered in Phosphate 1L 19.00 cart
RT 15740-04 Formalin 10% Buffered in Phosphate 4L 56.00 cart

arrow12Formamide, Ultra High Purity

HCONH2   F.W. 45.04   CAS #75-12-7
Specific Gravity: 1.128-1.132
Glass Distilled. Exceeds A.C.S. Specifications
Specifications:

Assay   99.9%
Iron.   0.5ppm
Copper   0.1ppm
Lead   0.5ppm
Zinc   0.5ppm
RT 15745 Formamide, Ultra High Purity 100ml 42.00 cart
RT 15746 Formamide, Ultra High Purity 500ml 124.00 cart
RT 15747 Formamide, Ultra High Purity 4x500ml 356.00 cart

msdsarrow12Formic Acid 96%, Reagent, A.C.S.

HCOOH   F.W. 46.03   CAS #64-18-6
Specific Gravity: 1.22

RT 15750 Formic Acid 96% 225ml 9.00 cart
RT 15760 Formic Acid 96% 1 liter 28.00 cart

msdsarrow12Formvar 15/ 95 Resin, Powder

CAS #63450-15-7
Polyvinyl formvar desiccated. Used to prepare Support films.
Soluble in ethylene dichloride, chloroform or dioxane.

RT 15800 Formvar 15/ 95 Resin 100g 10.00 cart

msdsarrow12Formvar Solution in Ethylene Dichloride

Microfiltered-ready to use.

RT 15810 0.25% 100ml 9.00 cart
RT 15820 0.50% 100ml 10.00 cart
RT 15830 1.00% 100ml 11.00 cart
RT 15830-25 1.00% 4x25ml 13.50 cart

*Any of the above percentages are packed in 25ml bottles to avoid its classification as a hazardous chemical for shipping purposes.  Please specify % upon ordering.

arrow12Gach Kit

Please see listing in Embedding Media Kits.

RT 15920 Giemsa Stain Solution kit 35.00 cart

Technical Tip:

Preparation of Gelatin Adhesive

msdsarrow12Gelatin, Powder, U.S.P.

CAS #9000-70-8
Solubility: 12% in H2O at 20°C
pH (1% in H2O): 3.5-5.0
Residue after ignition: <2%
Type A-from Porcine skin (acid cured tissue)
Type B-from Bovine skin (base cured tissue)
The strength of the gel is based on the bloom number. The higher the bloom number the stronger the gel.

  • Used as a combination Acrylamide-Gelatin-Jung resin for cryosectioning of large and soft tissues (eggs, fishes, insects) Hartmann, R. (1984). A new embedding medium for cryosectioning eggs of high yolk and lipid content. Eur. J. Cell Biol., 34:206
  • Used to prepare gelatin-chrome alum adhesive to coat glass slides for mounting semi-thin sections for staining.
RT 16560 Gelatin, Type A; × 60 Bloom 100g 23.00 cart
RT 16562 Gelatin, Type A; × 175 Bloom 100g 23.00 cart
RT 16564 Gelatin, Type A; × 300 Bloom 100g 23.00 cart
RT 16566 Gelatin, Type B; × 75 Bloom 100g 23.00 cart
RT 16568 Gelatin, Type B; × 225 Bloom 100g 23.00 cart

msdsHistocryl Resin arrow12Giemsa Solution

Glycerin (CAS#: 56-81-5): 61%
Methyl Alcohol (CAS#: 67-56-1): 38.3%
Azur II (CAS#: 37247-10-2): 0.16%
Azur II Eosin (CAS#: 53092-85-6): 0.06%

Specific Gravity: 0.89
Differentiates types of leucocytes, bacteria and inclusion bodies.
Giemsa, Centralbl. F. Publ., Abt. 1,37, 308 (1904) Stain for thick blood films; shows malarial parasites. Barber and Komp, Publ. Health Rep., 44, 2330 (1929) Bone marrow and stain for showing cell morphology; together with May-Grunwald Solution for pap staining. Pappenheim, Folia Haemat., 13, 338 (1912)

RT 15940 Giemsa Stain Solution 100ml 18.00 cart

msdsarrow12Giemsa Stain, Certified, Powder

CAS #51811-82-6
See the listing in Certified Dyes/Stains

RT 15942   10g 37.00 cart

arrow12For our Giesma Stain Solution

See the listing in Histology: Certified Dyes/Stains

msdsarrowGill’s Hematoxylin Staining Solution

Gill's Hematoxylin is a specially formulated solution for use as a biological stain for the nuclei and nuclear chromatin of normal and abnormal cells whether in whole or sliced cells. Our Gill's Hematoxylin is designed to use at its full strength. It is microfiltered prior to shipment; therefore, filtration before use is not required. For repeated use further filtration is recommended. At the discretion of the user, the Gill's Hematoxylin No. 2 and No. 3 may be diluted with ethylene glycol (25% is recommended) to produce a weaker stain solution.

RT 26030-10 Gill's Hematoxylin No. 1 1 L 42.00 Add to Cart
RT 26030-14 Gill's Hematoxylin No. 1 4 L 152.00 Add to Cart
RT 26030-20 Gill's Hematoxylin No. 2 1 L 44.00 Add to Cart
RT 26030-24 Gill's Hematoxylin No. 2 4 L 154.00 Add to Cart
RT 26030-30 Gill's Hematoxylin No. 3 1 L 46.00 Add to Cart
RT 26030-34 Gill's Hematoxylin No. 3 4 L 162.00 Add to Cart

Glutaraldehyde arrow12arrow12

logo22 About Us | Product Index | Privacy Policy | Contact Us | ©2003-2010 Electron Microscopy Sciences