Chemicals for Microscopy

Glutaraldehyde - The Highest Purity

Glutaraldehyde

OCHCH₂CH₂CH₂CHO F.W. 100.12 CAS #111-30-8
Specific Gravity @20/20°C: 1.131

EM Grade Distillation Purified

The Universal Fixative for Electron Microscopy.
Purified to EM Grade from commercial grade by "all-glass vacuum-distillation."
THE HIGHEST PURITY, free from polymers and other contaminants.
Each lot is tested and assayed in our lab before we ship and guarantee its purity.
Sealed under dry nitrogen.
Refrigerated prior to shipment.

After glutaraldehyde has been purified to EM grade, it has a high risk of polymerizing if it is not handled properly. Electron Micro-scopy Sciences takes special precautions to assure a polymer-free, pure glutaraldehyde.

Every lot of our EM grade aqueous glutaraldehyde is assayed prior to shipping assuring you consistent purity and the highest possible quality. If stored properly, our Glutaraldehyde has a very long shelf life.

We package our aqueous glutaraldehyde in 10 ml, single dose ampoules, sealed under dry nitrogen, and kept refrigerated until it is shipped. We offer our glutaraldehyde in a wide range of percentages to simplify mixing procedures and avoiding waste.

EM grade aqueous glutaraldehyde, stored in volumes greater than 100 ml, with repeated defrosting and recapping, will polymerize in time, producing an absorption peak of 235nm rather than the 280nm of pure EM grade. The presence of polymerized glutaraldehyde greatly reduces its efficiency as a cross-linking agent, preventing proper fixation. For this reason, we provide 10 ml ampoules and 100 ml bottle quantities for larger applications.

8% Aqueous Solution

0-5°C	16000		10 ml ampoule	6.00	Add to Cart
0-5°C	16019		Box 10x2ml	18.00	Add to Cart
0-5°C	16020		Box 10x10 ml ampoules	22.00	Add to Cart
0-5°C	16021	same as 16020	10 bxs	190.00	Add to Cart
0-5°C	16022	same as 16020	20 bxs	360.00	Add to Cart
0-5°C	16010		100 ml bottle	19.00	Add to Cart

10% Aqueous Solution

0-5°C	16100	10 ml ampoule	each	6.00	Add to Cart
0-5°C	16120	Box 10x10 ml ampoules	box	24.00	Add to Cart
0-5°C	16121	same as 16120	10 bxs	220.00	Add to Cart
0-5°C	16122	same as 16120	20 bxs	399.00	Add to Cart
0-5°C	16110	100 ml bottle	each	20.00	Add to Cart

25% Aqueous Solution

0-5°C	16200		10 ml ampoule	7.00	Add to Cart
0-5°C	16220		Box 10x10 ml ampoules	27.00	Add to Cart
0-5°C	16221	same as 16220	10 bxs	250.00	Add to Cart
0-5°C	16222	same as 16220	20 bxs	475.00	Add to Cart
0-5°C	16210		100 ml bottle	20.00	Add to Cart

50% Aqueous Solution

0-5°C	16300		10 ml ampoule	7.50	Add to Cart
0-5°C	16320		Box 10x10 ml ampoules	39.00	Add to Cart
0-5°C	16321	same as 16320	10 bxs	350.00	Add to Cart
0-5°C	16322	same as 16320	20 bxs	640.00	Add to Cart
0-5°C	16310		100 ml bottle	38.00	Add to Cart

70% Aqueous Solution

0-5°C	16350	10 ml ampoule	8.50	Add to Cart
0-5°C	16360	box 10x10 ml ampoules	52.00	Add to Cart
0-5°C	16365	box 10x2ml ampoules	30.00	Add to Cart

Biological Grade

0-5°C	16400	25% Solution	450 ml	18.00	Add to Cart
0-5°C	16401	25% Solution	4x450 ml	68.00	Add to Cart
0-5°C	16410	25% Solution	1 gal.	72.00	Add to Cart
0-5°C	16411	25% Solution	4x1 gal.	260.00	Add to Cart
0-5°C	16500	50% Solution	450 ml	21.00	Add to Cart
0-5°C	16501	50% Solution	4x450 ml	76.00	Add to Cart
0-5°C	16520	50% Solution	1 liter	39.00	Add to Cart
0-5°C	16521	50% Solution	4x1 liter	140.00	Add to Cart

Serum Vial Aqueous Glutaraldehyde

Over the years, many of our valued customers have requested purchasing our aqueous Glutaraldehyde in serum vials. This packaging allows the user to puncture the special non-reactive seal with a hypodermic syringe and draw out the exact quantity of aqueous Glutaraldehyde needed without opening the container. This packaging assures freshness and purity of the aqueous Glutaraldehyde at all times.

0-5°C	16216	25% EM Grade Aqueous Glutaraldehyde in Serum Vial	10 x10 ml	38.00	Add to Cart
0-5°C	16214	25% EM Grade Aqueous Glutaraldehyde in Serum Vial	100 ml	29.00	Add to Cart
0-5°C	16316	50% EM Grade Aqueous Glutaraldehyde in Serum Vial	10 x10 ml	49.00	Add to Cart
0-5°C	16314	50% EM Grade Aqueous Glutaraldehyde in Serum Vial	100 ml	39.00	Add to Cart

* All other concentrations are available in serum vials upon request.

For any special concentration, or packaging requirements other than those already listed, please call us today. We will manufacture in accordance to your specifications.

Anhydrous EM Grade Glutaraldehyde: Specially Prepared-10% Solution

Rapid-Freezing on a polished metal surface, at the temp. of liquid helium or liquid nitrogen, followed by freeze-substitution in osmium-acetone, has been shown to be a very useful technique for capturing rapid cellular events and for preserving cell ultrastructure.
* John M. Robinson and Morris J. Karnovsky, 1991. "Rapid-freezing Cytochemistry: Preservation of Tubular Lysosomes and Enzyme Activity". J. of Histochem., Cytochem. Vol. 39, No. 6, pp 787-792

Ordering Information: (please call for additional information)

16530	10% in Acetone	10x10 ml	80.00	Add to Cart
16531	10% in Ethanol	10x10 ml	80.00	Add to Cart
16532	10% in Methanol	10x10 ml	80.00	Add to Cart
16533	10% in Solvent of your choice	10x10 ml	80.00	Add to Cart

We would be pleased to fill your specific glutaraldehyde needs. Please call for prices on special concentrations or size requirements.

Glutaraldehyde, Pre-Made, Ready-to-Use, in Buffer

Glutaraldehyde 0.625% in 0.1M Phosphate Buffer, pH 7.4

RT	16535-05	Glutaraldehyde 0.625% in Phosphate Buffer, pH 7.4	500 ml	134.00	Add to Cart
RT	16535-10	Glutaraldehyde 0.625% in Phosphate Buffer, pH 7.4	1 L	262.00	Add to Cart

Glutaraldehyde 2% in 0.1M Phosphate Buffer, pH 7.4

RT	16536-05	Glutaraldehyde 2% in Phosphate Buffer, pH 7.4	500 ml	136.00	Add to Cart
RT	16536-10	Glutaraldehyde 2% in Phosphate Buffer, pH 7.4	1 L	272.00	Add to Cart

Glutaraldehyde 2% in 0.1M Sodium Cacodylate Buffer, pH 7.4

RT	16536-15	Glutaraldehyde 2% in Sodium Cacodylate Buffer, pH 7.4	500 ml	146.00	Add to Cart
RT	16536-20	Glutaraldehyde 2% in Sodium Cacodylate Buffer, pH 7.4	1 l	282.00	Add to Cart

Glutaraldehyde 2.5% in 0.1M Phosphate Buffer, pH 7.4

RT	16537-05	Glutaraldehyde 2.5% in Phosphate Buffer, pH 7.4	500 ml	152.00	Add to Cart
RT	16537-10	Glutaraldehyde 2.5% in Phosphate Buffer, pH 7.4	1 l	286.00	Add to Cart

Glutaraldehyde 2.5% in 0.1M Sodium Cacodylate Buffer, pH 7.4

RT	16537-15	Glutaraldehyde 2.5% in Sodium Cacodylate Buffer, pH 7.4	500 ml	152.00	Add to Cart
RT	16537-20	Glutaraldehyde 2.5% in Sodium Cacodylate Buffer, pH 7.4	500 ml	292.00	Add to Cart

Glutaraldehyde 3% in 0.1M Phosphate Buffer, pH 7.4

RT	16538-05	Glutaraldehyde 3% in Phosphate Buffer, pH 7.4	500 ml	158.00	Add to Cart
RT	16538-10	Glutaraldehyde 3% in Phosphate Buffer, pH 7.4	1 l	292.00	Add to Cart

Glutaraldehyde 3% in 0.1M Sodium Cacodylate Buffer, pH 7.4

RT	16538-15	Glutaraldehyde 3% in Sodium Cacodylate Buffer, pH 7.4	500 ml	162.00	Add to Cart
RT	16538-20	Glutaraldehyde 3% in Sodium Cacodylate Buffer, pH 7.4	1 l	298.00	Add to Cart

Glutaraldehyde 3% Sodium Phosphate Buffer, pH 7.2

RT	16539-45	Glutaraldehyde 3% in Sodium Phosphate Buffer, pH 7.2	10x10 ml	34.00	Add to Cart
RT	16539-50	Glutaraldehyde 3% in Sodium Phosphate Buffer, pH 7.2	500 ml	132.00	Add to Cart
RT	16539-55	Glutaraldehyde 3% in Sodium Phosphate Buffer, pH 7.2	1 L	272.00	Add to Cart

Glutaraldehyde 4% in 0.1m Phosphate Buffer, pH 7.3

RT	16539-60	Glutaraldehyde 4% in 0.1m Phosphate Buffer, pH 7.3	10x10 ml	34.00	Add to Cart
RT	16539-65	Glutaraldehyde 4% in 0.1m Phosphate Buffer, pH 7.3	500 ml	130.00	Add to Cart
RT	16539-70	Glutaraldehyde 4% in 0.1m Phosphate Buffer, pH 7.3	1 L	270.00	Add to Cart

Glutaraldehyde 2% Paraformaldehyde 2% in Phosphate Buffer, pH 7.4

RT	16536-06	Glutaraldehyde 2% Paraformaldehyde 2% in Phosphate Buffer, pH 7.4	100 ml	34.00	Add to Cart
RT	16536-07	Glutaraldehyde 2% Paraformaldehyde 2% in Phosphate Buffer, pH 7.4	500 ml	152.00	Add to Cart
RT	16536-08	Glutaraldehyde 2% Paraformaldehyde 2% in Phosphate Buffer, pH 7.4	1 Liter	278.00	Add to Cart

Glutaraldehyde 2.5% Paraformaldehyde 1% in Phosphate Buffer, pH 7.4

RT	16537-06	Glutaraldehyde 2.5% Paraformaldehyde 1% in Phosphate Buffer, pH 7.4	100 ml	32.00	Add to Cart
RT	16537-07	Glutaraldehyde 2.5% Paraformaldehyde 1% in Phosphate Buffer, pH 7.4	500 ml	150.00	Add to Cart
RT	16537-08	Glutaraldehyde 2.5% Paraformaldehyde 1% in Phosphate Buffer, pH 7.4	1 Liter	276.00	Add to Cart

Glutaraldehyde 2.5% Aqueous

RT	16537-16	Glutaraldehyde 2.5% , Aqueous	10x10 ml	21.00	Add to Cart
RT	16537-17	Glutaraldehyde 2.5% Aqueous	500 ml	62.00	Add to Cart
RT	16537-18	Glutaraldehyde 2.5% Aqueous	1 Liter	112.00	Add to Cart

Glutaraldehyde 3.0% Aqueous

RT	16537-19	Glutaraldehyde 3% Aqueous	100 ml	22.00	Add to Cart
RT	16537-20-S	Glutaraldehyde 3% Aqueous	500 ml	62.00	Add to Cart
RT	16537-21	Glutaraldehyde 3% Aqueous	1 Liter	112.00	Add to Cart

Glutaraldehyde 3.0% Paraformaldehyde3% in 0.1M Phosphate Buffer, pH 7.4

RT	16538-06	Glutaraldehyde 3% Paraformaldehyde 3% in Phosphate Buffer, pH 7.4	10x10 ml	22.00	Add to Cart
RT	16538-07	Glutaraldehyde 3% Paraformaldehyde 3% in Phosphate Buffer, pH 7.4	500 ml	62.00	Add to Cart
RT	16538-08	Glutaraldehyde 3% Paraformaldehyde 3% in Phosphate Buffer, pH 7.4	1 Liter	92.00	Add to Cart

Glutaraldehyde 4% in 0.1M in Sodium Cacodylate Buffer, pH 7.4

RT	16539-06	Glutaraldehyde 4% in 0.1M Sodium Cacodylate Buffer, pH 7.4	10x10 ml	34.00	Add to Cart
RT	16539-07	Glutaraldehyde 4% in 0.1M Sodium Cacodylate Buffer, pH 7.4	500 ml	132.00	Add to Cart
RT	16539-08	Glutaraldehyde 4% in 0.1M Sodium Cacodylate Buffer, pH 7.4	1 Liter	272.00	Add to Cart

Glutaraldehyde 5% Aqueous

RT	16539-15	Glutaraldehyde 5% Aqueous	10x10 ml	22.00	Add to Cart
RT	16539-20	Glutaraldehyde 5% Aqueous	500 ml	62.00	Add to Cart
RT	16539-25	Glutaraldehyde 5% Aqueous	1 Liter	112.00	Add to Cart

Glutaraldehyde 5% in 0.1M Phosphate Buffer, pH 7.4

RT	16539-30	Glutaraldehyde 5% in 0.1M Phosphate Buffer, pH 7.4	10x10 ml	34.00	Add to Cart
RT	16539-35	Glutaraldehyde 5% in 0.1M Phosphate Buffer, pH 7.4	500 ml	132.00	Add to Cart
RT	16539-40	Glutaraldehyde 5% in 0.1M Phosphate Buffer, pH 7.4	1 Liter	272.00	Add to Cart

Glutaraldehyde/Formaldehyde

Fixative (Karnovsky’s)
This fixative contains 3% glutaraldehyde and 2% formaldehyde in 0.1M phosphate buffer, pH 7.4

Karnovsky’s with formalin 2% and Biological glutaraldehyde 3% in 0.1m Phosphate Buffer

15731-10

Karnovsky Fixative Reagent Grade

1 L

245.00

Add to Cart

Karnovsky’s with paraformaldehyde solution and EM grade glutaraldehyde

Please Note: This kit can be formulated to fit your requirements. Please contact us.

15732-10

Karnovsky Fixative EM Grade

1 L

270.00

Add to Cart

Glutaraldehyde Spill Control; G-gard-ER

Transforms glutaraldehyde to a nearly neutral solution and renders the solution acceptable for sewer disposal. Prepares glutaraldehyde for biodegradation. Reduces biocidal activity and odor. 65g G-gard pre-treats one gallon of 1% solution.

RT	24142-01	Glutaraldehyde Spill Control	2.2oz	6.00	Add to Cart
RT	24142-10	Glutaraldehyde Spill Control	10 x 2.2oz	25.00	Add to Cart

Ampoule Cracker™

The Ampoule Cracker™ is a disposable plastic fracture and safety collar, which is specially designed to aid in the breaking open of pre-scored glass ampoules. Simply slip the Ampoule Cracker™ over the top at the pre-scored portion, snap it, and discard both the ampoule top and the safety collar to prevent cross contamination

60605-04	Ampoule Cracker for Ampoule Size 1 – 4 ml	500/pk	36.00	Add to Cart
60605-10	Ampoule Cracker for Ampoule Size 5 – 10 ml	500/pk	36.00	Add to Cart
60605-20	Ampoule Cracker for Ampoule Size 10 – 20 ml	500/pk	69.00	Add to Cart

Break-Safe™ Ampoule Opener

EMS Introduces Break-Safe™ allowing you to safely open up to 3 ampoules at one time

Box style design allows one handed opening of up to 3 ampules at a time. Gently pressing down on the lid snaps ampule necks clean while keeping them contained within the device and away from fingers. After removing ampule contents, simply turn the device upside down over a trash receptacle to safely discard ampules and tops.

Features:

Includes 4 inserts to allow opening of 1ml, 2ml, 5ml and 10 ml ampules
Open ampules easily and safely
Transparency allows unobstructed viewing of ampule contents at all times
Copolyester plastic construction has broad chemical resistance

60607

Break-Safe™ Ampoule Opener

each

40.00

Add to Cart

Ampoule Breaker; Soft Silicon

Reusable – Made from soft silicone. It offers a simple way to break pre-scored glass ampoules safely. Accommodates 5, 7, and 10 ml size ampoules. Reduces injury and spillage.

60600

Ampoule Breaker

each

15.00

Add to Cart

HISTOCHOICE® Clearing Agent

Histology Grade - Non flammable, replacement for Xylene for all tissue clearing needs

64114-01	Histochoice Clearing Agent	4 L	50.00	Add to Cart
64114-04	Histochoice Clearing Agent	4x4 L	195.00	Add to Cart

HISTOCHOICE™MB® *- The Molecular Biology Fixative

SAFE - Contains no Formaldehyde, Glutaraldehyde or Mercury.
NON-TOXIC – Is completely odorless and can be safely disposed
of down the drain.

HISTOCHOICE™ MB® Tissue Fixative is the first fixative designed for the molecular biologist. It is specialty formulated to preserve antigenic sites for antibody probes and nucleic acid sites for immunohistochemistry. HISTOCHOICE™MB® replaces formaldehyde based, alcohol based, Zenkers, B-5, B-3, Bouin’s and other fixatives with superior results. Tissue fixed in HISTOCHOICE™MB® exhibit vibrant staining, better nuclear and cytoplasmic detail, and will retain a crisp appearance even after long-term fixation.

Because this formula is designed specially for molecular biology applications, HISTOCHOICE™MB® fixed sections do not require pre-digestion or other recovery procedures to make important sites available.This means you spend less time preparing slides and more time doing research.

More than a fixative, HISTOCHOICE™MB® is a preservative that leaves antigens and nucleic acids in their native state, allowing binding for specific probes. Primary antibodies can often be diluted several-fold due to the increased number of preserved antigenic sites, saving cost
on every slide you process.

HISTOCHOICE™MB® fixed tissue retains a much more natural look and feel than formalin fixed tissue. It is important to remember that these tissues are not fixed by formalin, but rather preserved by HISTOCHOICE™MB®.

RT	64115-01	HISTOCHOICE™MB® Fixative	1 L	53.00	Add to Cart
RT	64115-04	HISTOCHOICE™MB® Fixative	4 L	175.00	Add to Cart
RT	64115-44	HISTOCHOICE™MB® Fixative	4x4 L	595.00	Add to Cart

* HISTOCHOICE is registered name of Amresco

Hollande’s Fixative

A modification of Bouin's solution. It is stable and will decalcify small bone specimens. Tissue that is fixed with Hollande's can be stained successfully with most stains, and the cupric acetate in the solution stabilizes red blood cell membranes and cosinophil and endocrine cell granules so that less lysis occurs than with Bouin's solution. Hollande's is widely used as a fixative for biopsy specimens of the gastrointestinal tract.

Thorough washing of the fixative prior to placing the specimen in a phosphate buffered formalin solution is necessary because the salts present in the solution will form an insoluble phosphate precipitate. Packaged in leak-proof histo-containers; prefilled ready-to-use.

	Catalog #	Cup Size	Filled	Pack/cs	Price
RT	64120-10	15 ml	10 ml	50	76.00	Add to Cart
RT	64120-20	30 ml	20 ml	50	96.00	Add to Cart
RT	64120-30	60 ml	30 ml	20	44.00	Add to Cart

Bulk packaging is available:

RT	64120-01	Hollande's Fixative	1 L	41.00	Add to Cart
RT	64120-04	Hollande's Fixative	4 L	130.00	Add to Cart

McDowell Trumps Fixative

RT	18030-05	McDowell Trumps Fixative	500 ml	30.00	Add to Cart
RT	18030-10	McDowell Trumps Fixative	1 L	36.00	Add to Cart

Michel’s Fixative, pH 7.0 – 7.4

RT	18040-05	Michel’s Fixative	500 ml	46.00	Add to Cart
RT	18040-10	Michel’s Fixative	1 L	81.00	Add to Cart
RT	18040-20	Michel’s Fixative Pre-filled	20 x10 ml	71.00	Add to Cart

Technical Tip:

Preparation of Tissue Biopsy in Michel's Fixative.

Mirsky’s Fixative™

Saponin/Glyoxylate Fixative
Preserves Immunohistological activity
Contains no formaldehyde
Fast-acting

Mirsky’s Fixative™ is a superior fixing agent for use in immunohistological and immunological protocols. Mirsky’s contains no formaldehyde or glutaraldehyde. Therefore, it has considerably reduced toxicity and virtually no odor. Mirsky’s Fixative is neutral, buffered, and isotonic (308 mOsmol). Additionally, Mirsky’s Fixative does not contain toxic or hazardous buffers such as cacodylate or barbital. Hardening or shrinkage of tissue is considerably reduced.

Double reactive sites afford excellent cross-linking properties while maintaining sample enzyme activity. Samples processed in Mirsky’s Fixative for light microscopy can subsequently be used in electron microscopy procedures. Special buffer systems may be used in place of the buffer provided.
Use as replacement for formalin and/or glutaraldehyde fixatives in immunohistological and immnocytological staining protocols. Tissue size is unlimited, as long as the sample is no thicker than 0.5cm in at least one plane to assure uniformity of tissue penetration. The gross visual appearance will be different than in formaldehyde and tissue may seem “raw”. This is due to double site binding of the fixative with a resultant reduction in tissue shrinkage. Microscopic examination of tissue morphology will be noticeably improved. This material is intended to maintain enzyme and antibody activity. For best results, tissue section of high digestive enzyme content should be thoroughly rinsed in saline solution before fixation (e.g. trypsin in intestinal samples). Once fixed, tissue may be retained in Mirsky’s Fixative and ethanol indefinitely.

Mirsky’s Fixative is normally distributed as a concentrated two bottle system, although high through-put laboratories often use the single bottle ready-to-use format. The two bottle system is comprised of Mirsky’s Fixative 10X Concentrated and Mirsky’s Fixative 10X Buffer. To reconstitute to working strength, add 1 part Mirsky’s Fixative 10X Buffer to 8 parts distilled or deionized water, mix thoroughly, then add 1 part Mirsky’s Fixative 10X Concentrate and mix again.

The two bottle system has a shelf life of 12 months while the ready-to-use format has a shel life of 30 days.

RT	15560	Mirsky’s Fixative System	200 ml	36.00	Add to Cart
RT	15561-05	Mirsky’s Fixative Ready-to-Use	500 ml	17.00	Add to Cart
RT	15561-10	Mirsky’s Fixative Ready-to-Use	1L	32.00	Add to Cart
RT	15561-20	Mirsky’s Fixative Ready-to-Use	2L	60.00	Add to Cart

RT	20350	Propylene Glycol, Absolute	450 ml	27.00	Add to Cart
RT	22400-1	1% Uranyl Acetate Solution	100 ml	30.00	Add to Cart
RT	22400-2	2% Uranyl Acetate Solution	100 ml	42.00	Add to Cart
RT	22400-3	3% Uranyl Acetate Solution	100 ml	56.00	Add to Cart
RT	22400-4	4% Uranyl Acetate Solution	100 ml	68.00	Add to Cart

Russell’s Zenker Fixative (Russel’s Modified Zenker)

Due to the hazardous nature of mercury, Zenker’s fluid is no longer available. In place we are offering Russell’s Zenker Fixative, which contains Zinc Chloride. The staining results produced by this fixative are comparable to those of Zenker’s Stock solution. Russell’s Modified Zenker’s is used in the same manner as Zenker’s stock solution.

RT	64123-05	Russell’s Zenker Fixative	500 ml	25.00	Add to Cart
RT	64123-06	Russell’s Zenker Fixative	1 L	42.00	Add to Cart

Technical Tips

The Use of Glutaraldehyde, Acrolein and Paraformaldehyde Buffer

Glycerine to HPMA

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Electron Microscopy Sciences specializes in the manufacturing, preparation and distribution of the highest quality laboratory chemicals and microscopy supplies and equipment for electron microscopy, light microscopy, and histology.