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Chemicals for Microscopy

Electron Microscopy Sciences

arrow14 Glycerine to HPMA

arrow12Glycerine, Reagent, A.C.S.

(Glycerol)
C3H5(OH)3   F.W. 92.10   CAS #56-81-5

Specific Gravity: 1.23    
Assay   <99.5%
Residue after Ignition   0.005%
Chlorinated Compounds(as Cl)   0.003%
Sulfate   To Pass Test
Heavy Metals (as Pb)   0.0002%
RT 16550   450ml 35.00 cart

arrow12(GMA), Glycol-Methacrylate, Low Acid

(2-Hydroxyethyl methacrylate monomer, inhibited;HEMA)
C6H10O3   F.W. 130.14   CAS#868-77-9
Specific Gravity: 1.07
b.p. 67°C
Inhibited with approximately 330ppm MEHQ
A water soluble monomer.

RT 16800   450ml 30.00 cart

Article of Interest

Preparation of Glycol Methacrylate

arrow12GMA Embedding Kit

Please see listing in Embedding Media Kits.

  14200   kit 46.00 cart

arrow12GMA-PEG Embedding Kit Polyethylene Glycol- Glycol methacrylate mixture.

Please see listing in Embedding Media Kits.

  14250   kit 47.00 cart

arrow12Gold Chloride, Hydrate(Chloroauric Acid; Hydrogen Tetrachloroaurate)

H(AuCl4)·H2O   F.W. 357.79   CAS #16961-25-4
Assay 99.9985%
Gold contents 49%+
A highly purified gold compound for the preparation of Colloidal Gold sols. Preweighed to facilitate your work. (Preparation instructions for Gold Sols included)

RT 16580   10x1/10g 36.00/bx cart
RT 16582   10x1/2g 176.00/bx cart
RT 16583   1g 36.00/g cart
RT 16584   10x1g 350.00/bx cart

arrow12Gold Chloride Solutions

Please see listing in Reagents for Histology .

arrow12Tannic Acid, used to prepare gold sols

Please see listing in Chemicals.

arrowGram's Iodine

For use as a bacterial, fungal and inclusion body stain.

RT 26054-05 Gram's Iodine Solution 500ml 20.00 Add to Cart
RT 26054-06 Gram's Iodine Solution 1 L 31.00 Add to Cart

arrow12Graphite Adhesives

Please see listing in Colloidal Compounds.

arrow12Gum Mastic

Please see listing in Reagents for Histology .

arrow12HANKS's Balanced Salt Solution (HBSS)

All of our Hanks’ Balanced Salt Solutions contain: Potassium Chloride, Potassium Phosphate Monobasic, Sodium Chloride, Sodium Phosphate Dibasic, and D-Glucose. Some formulations may contain additional components. Use the table below as a quick reference for identifying the one that best suits your application. All solutions are prepared in 1X strength.

Ingredients F.W. Formula I Formula II Formula III
KCl 74.56 X X X
NaCl 58.44 X X X
Na2HPO4. 7H2O 268.07 X X X
D-Glusose 180.16 2 g/L 1 g/L ----
MgSO4. 7H2O 246.48 X X X
CaCl2, 2H2O 147.02 X X X
NaHCO3 84.01 X X X
KH2PO4 136.09 X X X
Phenol Red   0.02 g/L 0.02 g/L 0.015 g/L
RT 11768-05 HBSS, Formula I 500ml 16.00 cart
RT 11768-10 HBSS, Formula I 1 L 21.00 cart
RT 11769-05 HBSS, Formula II 500ml 16.00 cart
RT 11769-10 HBSS, Formula II 1 L 21.00 cart
RT 11770-05 HBSS, Formula III 500ml 16.00 cart
RT 11770-10 HBSS, Formula III 1 L 21.00 cart

arrowHarris’ Hematoxylin

Stain selectivity for nuclei, fat and lipids. Ready-to-use.

RT 26041-05 Harris' Hematoxylin 500ml 32.00 Add to Cart
RT 26041-06 Harris' Hematoxylin 1 L 55.00 Add to Cart
RT 26041-20 Harris Hematoxylin without Mercury 500 ml 30.00 Add to Cart

arrow12Hematoxylin, Certified, C.I.#75290

(Natural Black I, Hydroxybrasilin)
C16H14O6   F.W. 302.29   CAS #517-28-2
Solubility: 10.0% Water; 10.0% Alcohol; 9.5% Cellosolve; 10% Glycol; 0.0% Xylene. A certified biological stain used in histology and cytology and plastic embedded tissues.
Chen, Chang, S., J Arch. of Path., 93,334 (1972).

RT 16620   10g 24.00 cart

Technical Tip

Hematoxylin Staining Tips

arrow12Hepes 0.2M Buffer Solution

Please see listing in Prepared Buffers.

RT 11494   500ml 16.00 cart

arrow12Hepes, Crystal, Biological Buffer

(N-2-Hydroxyethylpiperazine-N'-2-Ethanesulfonic acid)
C8H18O4N2S   F.W. 238.30   CAS #7365-45-9

Assay   >99%
pKa @20°C   7.55
pH Range   7.0-8.0
A(260) (1.0M in H2O)   <0.05
Ash   <0.1%
Good's buffer.    
RT 16780   25g 14.50 cart
RT 16782   100g 52.00 cart

arrow12(HMDS), Hexamethyldisilazane, Reagent

(CH3)3SiNHSi(CH3)3   F.W. 161.40   CAS #999-97-3
b.p. 126.7°C
Assay >97.0%
Specific Gravity @25°C 0.774
Refractive Index @20°C: 1.407
* For fast preparation of soft insect tissues for SEM. A good substitution for critical point drying. Int. J. Insect Morh. Embryol., 12(4), 201 (1983) Stain Tech., 58(6), 347 (1983)
* Bray, D.F., Et-al. (1993). Micros. Res. and Technique. 26:489-495. A comparison of HMDS, Peldri II, and critical point drying methods for SEM of biological specimens.

RT 16700   100ml 17.00 cart

arrow12,Hexamethylenetetramine A.C.S. Reagent, Granular

(Urotropine; Methenamine)
C6N4   F.W. 140.19   CAS #100-97-0
Assay: 99.0-100.5%
Specifications:

Residue after Ignition   0.1%
Chloride   0.014%
Sulfate   To Pass Test
Heavy Metals.   0.001%

Used in silver stains.
Swift, J.A. (1968) Microsc. Soc. 88, 449.

RT 16710   50g 11.00 cart
RT 16710-25*   25g 6.00 cart

*We are now offering Hexamethylenetetramine in 25 ml bottles to avoid its classification as a hazardous chemical for shipping purposes.

arrow12Hexylene Glycol, Reagent, A.C.S.

(2-Methyl-2,4-pentanediol)
(CH3)2C(OH)CH2CH(OH)CH3   F.W. 118.18   CAS #107-41-5
Specific Gravity @25°C 0.923
A.C.S. Specifications:

Assay   99%
IR Spectrum   To Pass Test
Refractive Index @20°C   0.921-0.923

Mixed with water in all proportions. Used as a dehydrating agent for fat tissues.

RT 16740   450ml 23.00 cart
RT 16750   4x450ml 88.00 cart

arrow12HPMA Embedding Kit

Please see listing in Embedding Media Kits.

RT 14220   kit 39.00 cart

Hydrochloric to Mucicarmine arrow12arrow12

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