Electron Microscopy Sciences

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Chemicals for Microscopy

Electron Microscopy Sciences

arrow14 Hydrochloric to Mucicarmine

arrow12Hydrochloric Acid Solution 0.1N

HCl   CAS #7647-01-0
Used to adjust the pH of buffers and fixative solutions.

RT 16760   100ml 4.00 cart

arrow12Hydrochloric Acid Solution 1.0N

HCl   CAS #7647-01-0
Used to adjust the pH of buffers and fixative solutions.

RT 16770   100ml 5.00 cart

arrow12Hydrochloric Acid Solutions

For all our Hydrochloric Acid Solutions, please see listing in Reagents for Histology.

arrow12HydrogenPeroxide 30% Solution-Stabilized, Reagent, A.C.S.

H2O2   F.W. 34.01   CAS #7722-84-1

Assay   29-32%
Maximum of Impurities:    
Chloride(Cl)   0.0003%
Nitrate   0.0002%
Phosphate   0.0002%
Sulfate   0.0005%
Heavy Metal   0.0001%
Iron   0.00005%
Titratable Acid   0.0006meq/g
0-5ºC 16790   100ml 38.50 cart
0-5ºC 16792   450ml 130.00 cart

arrow122-Hydroxyethyl Methacrylate

Please see (GMA) Glycol methacrylate.

RT 16800   450ml 29.00 cart

arrow12Hydroquinone Solutions

For all our Hydroquinone Solutions, please see listing in Reagents for Histology.

arrow12(HPMA), 2-Hydroxypropyl Methacrylate

(Propylene glycol monomethacrylate)
C7H12O3   F.W. 144.17   CAS #27813-02-1
b.p. 70°C/1mmHg
Specific Gravity @25°C 1.028
Solubility in Water 13%
Inhibited with approx. 300ppm MEHQ
A water miscible monomer

0-5ºC 16900   450ml 25.00 cart
0-5ºC 16901   4x450ml 90.00 cart

arrow12Imidazole, Reagent

(Glyoxaline)
C3H4N2   F.W. 68.08   CAS #288-32-4
Assay min 99.5%
Imidazole is a lipid-enhancing buffer agent. When used with 4% osmium tetroxide solution in the post-fixation of tissue blocks, it produces a prominent electron-opaque staining of lipid droplets with no evidence of diffusion.
Reference: Angermuller, S. Fahimi, H., Histochem. Journal 14, 823-35 (1982)

RT 16905   100g 16.75 cart

Cargille PCB-Free Immersion oilsarrow12Immersion Oils:

Which Type - Which Application?

For Normal Light Microscopy:

Types A & B, with viscosities of 150 and 1250cSt respectively, meet these requirements. The greater the gap between cover glass and objective, or condenser and slide, the more desirable high viscosity becomes. For extremely large gaps, type NVH should be used. All Cargille Immersion Oils can be blended for intermediate viscosities.

arrow12Immersion Oils

Cargille PCB-Free Immersion oils were introduced in 1972. Today, no other immersion oil meets FDA criteria.  Now four standard types are available with the introduction of Type NVH, a very high viscosity oil.  All Cargille Immersion Oils meet DIN 58 884 (Deutsche Institut Fur Normung e.V.)

arrow1237ºC Immersion Oils

A number of research projects require microscopic studies at internal body temperature.   Since the index/temperature relationship is inverse, increasing temperature significantly reduces the index of the immersion oil and consequently the imagery deteriorates.  This problem has been resolved by the formation of Cargille 37ºC Immersion Oils, which have an index of 1.515 at 37ºC.

Consideration of these oils is also suggested where stage temperatures are elevated by sub-stage illuminators and high wattage projection equipment.

For Horizontal, Inverted and Inclined Instruments and Projection Equipment:

Higher viscosities are generally required.  Type NVH meets the requirement, having a viscosity of 21,000cST.

OPTICAL & PHYSICAL PROPERTIES
Immersion Oil Type A B NVH 37
Refractive Index : @23°C @23°C @23°C @23°C
  F Line (4861 A) 1.5239 1.5236 1.5230 1.5236*
  E Line (5461 A) 1.5180 1.5180 1.5178 1.5180*
  D Line (5893 A) 1.5150 1.5150 1.5118 1.5115*
  C Line (6563 A) 1.5115 1.5116 1.5118 1.5115*
Dispersion:          
  nF-nC 0.0124 0.0120 0.0112 0.0121*
  Abbe VD 41.6 42.9 46.0 42.4*
  Abbe Ve 41.2 42.6 45.7 42.2*
Temperature Coefficient (15-35°C) _ dnD/dt 0.00033 0.00031 0.00034 0.00035*
Stability (change in nD @25°C after 24 hrs at stated temperature) *        
  60°C: 0 0 0 0
  100°C: 0 0 0 0
Fluorescence*: Short UV: low low low low
Fluorescence*: Long UV: low low low low
Color: (Gardner) 1 1 1 1
Viscosity:  cSt +/- 10% @ 23°C (centistokes) 150
low
1250
high
21,000
very high
1250b
high
Density: @ 23°C gm/cc: .923 .923 .919 .927
Density: (US) lb/gal 7.70 7.70 7.67 7.73
Cloud Point: 0°C 0°C 0°C <-7.5°C
Flash Point: 325°F 325°F 325°F 325°F
Neutralization: (mg KOH/gm) 0.01 max 0.01 max 0.01 max 0.01 max
RT 16907-01 TYPE A 1oz. 5.90 cart
RT 16907 TYPE A 4oz. 13.00 cart
RT 16907-16 TYPE A 16oz. 28.50 cart
RT 16908-01 TYPE B 1oz. 5.60 cart
RT 16908 TYPE B 4oz. 12.75 cart
RT 16908-16 TYPE B 16oz. 27.00 cart
RT 16909-01 TYPE NVH 1oz. 7.50 cart
RT 16909 TYPE NVH 4oz. 16.40 cart
RT 16914-01 TYPE 37 1oz. 7.90 cart
RT 16909-04 TYPE 37 4oz. 17.30 cart
For Fluorescence Microscopy

After years of development and field testing, we now have available two new PCB-Free Immersion Oils for Fluorescence Microscopy.

* Immersion Oil Type DF

Type DF has the highest resolution of any fluorescent microscopy immersion oil. Its background is slightly green.

* Immersion Oil Type FF

Type FF has virtually no background fluorescence which makes it ideal for observing low levels of fluorescence. It is highly stable, water white, and non-hydroscopic. Type FF can be substituted for all other fluorescence microscopy immersion oils in nearly all fluorescence applications.

OPTICAL & PHYSICAL PROPERTIES
Immersion Oil Type DF Type FF
Refractive Index @23°C::
  F Line (4861 A) 1.5234 1.4850
  E Line (5461 A) 1.5180 1.4810
  D Line (5893 A) 1.5150 1.4790
  C Line (6563 A) 1.5118 1.4766
Dispersion:  
  nF-nC 0.0116 0.0084
  Abbe VD 44.3 57.3
  Abbe Ve 44.0 56.8
Temperature Coefficient (15-35°C) _ dnD/dt 0.00039 0.00037
Stability (change in nD @25°C after  24 hrs at stated temperature) *  
  60°C: 0 0
  100°C: +0.0002 0
Fluorescence*:    
  Short UV: Very-Very-Low None
  Long UV: Very-Low None
Color: (Gardner) <1 <1
Viscosity: Centistokes +/-10% @ 23°C 330 170
Density:    
  @ 23°C g/cc: 1.225 0.877
  (US) lb/gal 10.22 7.32
Cloud Point: <-6°C <-6°C
Flash Point: 390°F 420°F
Neutralization: (mg KOH/gm) 0.15max 0.01max

*Relative to Cedarwood Oil

RT 16915-01 Immersion Oil Type DF 1 oz.* 9.40 cart
RT 16915-04 Immersion Oil Type DF 4 oz. 20.70 cart
RT 16916-01 Immersion Oil Type FF 1 oz.* 9.00 cart
RT 16916-04 Immersion Oil Type FF 4 oz. 19.80 cart

* 1 oz. Applicator bottles have a glass ball rod in the cap.

arrow12Immuno-Bed™ Embedding Kit

Please see listing in Embedding Media Kits.

arrow12Indigo Carmine, Certified, C.I. #73015

(Acid Blue 74; Carmine Blue; Indigotine 1, 1A)
C16H8N2O8S2Na2   F.W.466.36   CAS #860-22-0
Dye content: approx. 85%
Solubility: 1.3% Water; 0.0% Alcohol; 4.0% Cellosolve; 2.85% Glycol; 0.0% Xylene
Shunway's stain for animal embryos. Used with Acid Fuchsin for staining Negri bodies. Stain Tech., 5, 34 (1928)
Counterstain to Hematoxylin for staining vaginal smears. Am. J. Anat., 61, 505 (1937).

RT 16910   25g 17.00 cart

Technical Tip

When a stain is applied both en-bloc and as a post-stain for thin sections, maximum contrast will be achieved.

arrow12Indium Trichloride, Anhydrous

InCl3   F.W. 221.18   CAS #10025-82-8
Chloride Content: 98%
A metal stain for nucleic acid
J. Biophys Biochem Cytol, 11,257 (1961)

42.00
RT 16920  5g 42.00 cart

arrow12Ingrain Blue, C.I. #74240

Please see Alcian Blue 8GX

42.00
RT 10350  25g 200.00 cart

arrow12Isopropyl Alcohol, Reagent, A.C.S.

(2-Propanol)
CH3CH(OH)CH3   F.W. 60.10   CAS #67-63-0
A.C.S. Specifications:

Color (APHA) . 10
Density @ 25°C . 0.781-0.783
Boiling Range (including 82.3°C) . 1°C
Titratable Acid or Base . 0.0001 meq/g
Residue after Evaporation . 0.001%
Solubility in water . To pass test
Water . 0.2%
RT 16930 1qt 12.00 cart
RT 16931 4x1qt 42.00 cart

arrow12JB-4(TM) Embedding Kit

Please see listing in Embedding Media Kits.

arrow12Karnovsky's Fixative

Please see Paraformaldehyde-Glutaraldehyde Solution.

Technical Tip

Elimination of Section Contamination During Staining

arrow Kinyoun's Solution

Kinyoun's carbol fuchsin staining solution for acid-fast bacteria.

RT 26087-05 Kinyoun's Solution 500 ml 56.00 Add to Cart
RT 26087-06 Kinyoun's Solution 1 L 90.00 Add to Cart

arrow12Lanthanum Nitrate, Hexahydrate, Reagent

La(NO3)3·6H20   F.W. 433.03   CAS #10277-43-7

m.p. .. 40°C
Assay .. Min 99.9%
Calcium .. 0.001%
Magnesium .. 0.0001%

A metal stain, intracellular tracer.

  • Lane, N,J. and Treherne, J.E. (1970). Lanthanum staining of neurotubules in axons from cockroach ganglia. J. Cell Sci., 7:217
  • Lane, N.J. (1972) Fine structure of a lipidopteran nervous system and its accessibility to peroxidase and lanthanum. Z. Zellforsch., 131:205
RT 17300 50g 28.00 cart

arrow12Lead Acetate, Trihydrate Reagent, A.C.S.

Pb(CH3COO)2·3H20   F.W. 379.33   CAS #6080-56-4
A.C.S. Specifications:

Assay . 99.0-103.0%
Chloride . 5ppm
Copper . 0.002%
Insoluble Matter .  0.01%
Iron . 0.001%
Nitrate and Nitrite (as NO3) . To pass test
0-5ºC 17600 100g 15.00 cart
0-5ºC 17600-25 25g 5.00 cart

*We are now offering Lead Acetate in 25ml bottles to avoid its classification as a hazardous chemical for shipping purposes.

arrow12Lead Citrate, Trihydrate

Pb(C6H5O7)2·3H2O   FW 1053.82   CAS #512-26-5
Minimum assay 99.%
The most widely used metal stain for ultra thin sections.
Reynolds, E.S., J. Cell Bio., 17, 208 (1963)
Sato, T., J. Electron Micro., 116, 133 (1976)
Venable, J.H. and Coggeshall, R. (1965). A simplified lead citrate stain for use in electron microscopy. J. Cell Biol., 25:407
Famy, A. (1967). An extemporaneous lead citrate for electron microscopy. Proc. 25th Ann. EMSA Meet., p.148. Claitor's Pub. Division, Baton Rouge, LA.

RT 17800 25g 17.00 cart
RT 17810 100g 52.00 cart

Technical Tips

Handling of Lead Solutions
Lead Staining
Common Lead Stock Solutions
A Stable Lead Staining Solution

arrow12Lead Nitrate, Reagent, A.C.S.

Pb(NO3)2   F.W. 331.21   CAS #10099-74-8

Minimum Assay 99.5%
Insoluble matter 0.005%
Reaction Not less than pH 3.5
Chloride 0.0005%
Calcium 0.01%
Copper 0.0005%
Iron 0.0005%
Potassium 0.002%
Sodium. 0.002%

A metal stain for ultra thin sections.
Sato, T., (1967) J. Electron Micro., 16, 733

RT 17900 25g 5.00 cart

arrow12Light Green SF Yellowish, Certified, C.I. #42095

(Acid Green 5; Light Green 2G, S or 2GN; Acid Green F; Fast Acid Green N)
C37H34N2Na2O9S3   F.W. 792.86   CAS #5141-20-8
Solubility: 20.35% Water; 4.0% Alcohol; 5.25% Cellosolve; 12.0% Glycol; 0.0% Xylene

RT 17920 10 g 15.00 cart

arrow Light Green SF Yellowish Solution

Light SF Yellowish, 0.2% in 0.2% acetic acid fo use in the GM Method, as a fungal stain.

RT 26085-05 Light Green SF Yellowish,
0.2% in 0.2% Acetic Acid
500ml 39.00 Add to Cart
RT 26085-06 Light Green SF Yellowish,
0.2% in 0.2% Acetic Acid
1 L 65.00 Add to Cart
RT 26085-10 Light Green SF Yellowish,
1% Aqueous
500 ml 48.00 Add to Cart

arrow12(SPURR), Low Viscosity, Embedding Kit

Please see listing in Embedding Media Kits.

RT 14300 kit 60.00 cart

arrow12Technical Tips

Embedding Resin Stain Permability

arrow12Lowicryl, Embedding Kit,

Please see listing in Embedding Media Kits

RT 14330 Lowicryl K4M kit 352.00 cart
RT 14340 Lowicryl HM20 kit 352.00 cart
RT 14350 Lowicryl K11M kit 352.00 cart
RT 14360 Lowicryl HM23 kit 352.00 cart

arrow12Lowicryl MonoStep Single Component Embedding Media

The same as our Lowicryl complete kits but this new formulation allows you one stop embedding without all of the cumbersome preparation and mixing.  This pre-mixed, ready to use resin, saves you time and minimizes chemical contact.  MonoStep Lowicryl is ideal for use in immunohistochemistry and immunolabeling.  Store at room temperature.

RT 14335 Lowicryl HM-20 Polar
Embedding Medium; MonoStep
225g 155.00 cart
RT 14345 Lowicryl HM-20 Polar
Embedding Medium; MonoStep
225mg 155.00 cart

arrow12LR White And LR Gold Resin

Please see listing in Embedding Media Kits.

RT 14370 LR Gold Complete kit 212.00 cart
RT 14380 LR White Complete kit 116.00 cart

Articles of Interest

Flat Embedding with LR-White

arrow12Lucifer Yellow, CH, Dilithium Salt

C13H9Li2N2O9S2   F.W. 457.25   CAS #67769-47-5

A technique producing an electron dense osmiophilic polymer when Lucifer Yellow is injected into cells. Lucifer Yellow also can be viewed in living cells as a dye marker and is more sensitive than any other dye.
Cell Tissue Res., 204, 37 (1979) J. Histochem Cystochem, 30, 189 (1982) Science, 217, 953 (1982); 215, 1641 (1982); 214, 801 (1981); 225, 851 (1984); Cell, 14, 741 (1978).
A fluorescent dye. Nature 292, 17 (1981)

0-5ºC 17950 25mg 96.00 cart

arrowLugol’s Iodine

For use as a bacterial, fungal and inclusion body stain.

RT 26055-05 Lugol's Iodine Solution 500ml 21.00 Add to Cart
RT 26055-06 Lugol's Iodine Solution 1L 36.00 Add to Cart

arrow12Magnesium Chloride, Hexahydrate, Reagent, A.C.S.

MgCl2·6H2O   F.W. 203.30   CAS #7791-18-6
Assay: 99.0-102.0%

RT 18010 100g 20.00 cart

arrow12Malachite Green 4, Certified

Please see Basic Green Oxalate

RT 18100 25g 38.00