HCl   CAS #7647-01-0
Used to adjust the pH of buffers and fixative solutions.

RT

16760

100ml

4.00

Hydrochloric Acid Solution 1.0N

HCl   CAS #7647-01-0
Used to adjust the pH of buffers and fixative solutions.

RT

16770

100ml

5.00

Hydrochloric Acid Solutions

For all our Hydrochloric Acid Solutions, please see listing in Reagents for Histology.

HydrogenPeroxide 30% Solution-Stabilized, Reagent, A.C.S.

H₂O₂   F.W. 34.01   CAS #7722-84-1

Assay		29-32%
Maximum of Impurities:
Chloride(Cl)		0.0003%
Nitrate		0.0002%
Phosphate		0.0002%
Sulfate		0.0005%
Heavy Metal		0.0001%
Iron		0.00005%
Titratable Acid		0.0006meq/g

0-5ºC	16790		100ml	38.50
0-5ºC	16792		450ml	130.00

2-Hydroxyethyl Methacrylate

Please see (GMA) Glycol methacrylate.

RT

16800

450ml

29.00

Hydroquinone Solutions

For all our Hydroquinone Solutions, please see listing in Reagents for Histology.

(HPMA), 2-Hydroxypropyl Methacrylate

(Propylene glycol monomethacrylate)
C₇H₁₂O₃   F.W. 144.17   CAS #27813-02-1
b.p. 70°C/1mmHg
Specific Gravity @25°C 1.028
Solubility in Water 13%
Inhibited with approx. 300ppm MEHQ
A water miscible monomer

0-5ºC	16900		450ml	25.00
0-5ºC	16901		4x450ml	90.00

Imidazole, Reagent

(Glyoxaline)
C₃H₄N₂   F.W. 68.08   CAS #288-32-4
Assay min 99.5%
Imidazole is a lipid-enhancing buffer agent. When used with 4% osmium tetroxide solution in the post-fixation of tissue blocks, it produces a prominent electron-opaque staining of lipid droplets with no evidence of diffusion.
Reference: Angermuller, S. Fahimi, H., Histochem. Journal 14, 823-35 (1982)

RT

16905

100g

16.75

Immersion Oils:

Which Type - Which Application?

For Normal Light Microscopy:

Types A & B, with viscosities of 150 and 1250cSt respectively, meet these requirements. The greater the gap between cover glass and objective, or condenser and slide, the more desirable high viscosity becomes. For extremely large gaps, type NVH should be used. All Cargille Immersion Oils can be blended for intermediate viscosities.

Immersion Oils

Cargille PCB-Free Immersion oils were introduced in 1972. Today, no other immersion oil meets FDA criteria.  Now four standard types are available with the introduction of Type NVH, a very high viscosity oil.  All Cargille Immersion Oils meet DIN 58 884 (Deutsche Institut Fur Normung e.V.)

37ºC Immersion Oils

A number of research projects require microscopic studies at internal body temperature.   Since the index/temperature relationship is inverse, increasing temperature significantly reduces the index of the immersion oil and consequently the imagery deteriorates.  This problem has been resolved by the formation of Cargille 37ºC Immersion Oils, which have an index of 1.515 at 37ºC.

Consideration of these oils is also suggested where stage temperatures are elevated by sub-stage illuminators and high wattage projection equipment.

For Horizontal, Inverted and Inclined Instruments and Projection Equipment:

Higher viscosities are generally required.  Type NVH meets the requirement, having a viscosity of 21,000cST.

OPTICAL & PHYSICAL PROPERTIES

Immersion Oil Type		A	B	NVH	37
Refractive Index :		@23°C	@23°C	@23°C	@23°C
	F Line (4861 A)	1.5239	1.5236	1.5230	1.5236*
	E Line (5461 A)	1.5180	1.5180	1.5178	1.5180*
	D Line (5893 A)	1.5150	1.5150	1.5118	1.5115*
	C Line (6563 A)	1.5115	1.5116	1.5118	1.5115*
Dispersion:
	nF-nC	0.0124	0.0120	0.0112	0.0121*
	Abbe VD	41.6	42.9	46.0	42.4*
	Abbe Ve	41.2	42.6	45.7	42.2*
Temperature Coefficient (15-35°C) _ dnD/dt		0.00033	0.00031	0.00034	0.00035*
Stability (change in nD @25°C after 24 hrs at stated temperature) *
	60°C:	0	0	0	0
	100°C:	0	0	0	0
Fluorescence*: Short UV:		low	low	low	low
Fluorescence*: Long UV:		low	low	low	low
Color: (Gardner)		1	1	1	1
Viscosity:  cSt +/- 10% @ 23°C (centistokes)		150 low	1250 high	21,000 very high	1250b high
Density: @ 23°C gm/cc:		.923	.923	.919	.927
Density: (US) lb/gal		7.70	7.70	7.67	7.73
Cloud Point:		0°C	0°C	0°C	<-7.5°C
Flash Point:		325°F	325°F	325°F	325°F
Neutralization: (mg KOH/gm)		0.01 max	0.01 max	0.01 max	0.01 max

RT	16907-01	TYPE A	1oz.	5.90
RT	16907	TYPE A	4oz.	13.00
RT	16907-16	TYPE A	16oz.	28.50
RT	16908-01	TYPE B	1oz.	5.60
RT	16908	TYPE B	4oz.	12.75
RT	16908-16	TYPE B	16oz.	27.00
RT	16909-01	TYPE NVH	1oz.	7.50
RT	16909	TYPE NVH	4oz.	16.40
RT	16914-01	TYPE 37	1oz.	7.90
RT	16909-04	TYPE 37	4oz.	17.30

For Fluorescence Microscopy

After years of development and field testing, we now have available two new PCB-Free Immersion Oils for Fluorescence Microscopy.

* Immersion Oil Type DF

Type DF has the highest resolution of any fluorescent microscopy immersion oil. Its background is slightly green.

* Immersion Oil Type FF

Type FF has virtually no background fluorescence which makes it ideal for observing low levels of fluorescence. It is highly stable, water white, and non-hydroscopic. Type FF can be substituted for all other fluorescence microscopy immersion oils in nearly all fluorescence applications.

OPTICAL & PHYSICAL PROPERTIES

Immersion Oil		Type DF	Type FF
Refractive Index @23°C::
	F Line (4861 A)	1.5234	1.4850
	E Line (5461 A)	1.5180	1.4810
	D Line (5893 A)	1.5150	1.4790
	C Line (6563 A)	1.5118	1.4766
Dispersion:
	nF-nC	0.0116	0.0084
	Abbe VD	44.3	57.3
	Abbe Ve	44.0	56.8
Temperature Coefficient (15-35°C) _ dnD/dt		0.00039	0.00037
Stability (change in nD @25°C after  24 hrs at stated temperature) *
	60°C:	0	0
	100°C:	+0.0002	0
Fluorescence*:
	Short UV:	Very-Very-Low	None
	Long UV:	Very-Low	None
Color: (Gardner)		<1	<1
Viscosity: Centistokes +/-10% @ 23°C		330	170
Density:
	@ 23°C g/cc:	1.225	0.877
	(US) lb/gal	10.22	7.32
Cloud Point:		<-6°C	<-6°C
Flash Point:		390°F	420°F
Neutralization: (mg KOH/gm)		0.15max	0.01max

*Relative to Cedarwood Oil

RT	16915-01	Immersion Oil Type DF	1 oz.*	9.40
RT	16915-04	Immersion Oil Type DF	4 oz.	20.70
RT	16916-01	Immersion Oil Type FF	1 oz.*	9.00
RT	16916-04	Immersion Oil Type FF	4 oz.	19.80

* 1 oz. Applicator bottles have a glass ball rod in the cap.

Immuno-Bed™ Embedding Kit

Please see listing in Embedding Media Kits.

Indigo Carmine, Certified, C.I. #73015

(Acid Blue 74; Carmine Blue; Indigotine 1, 1A)
C₁₆H₈N₂O₈S₂Na₂   F.W.466.36   CAS #860-22-0
Dye content: approx. 85%
Solubility: 1.3% Water; 0.0% Alcohol; 4.0% Cellosolve; 2.85% Glycol; 0.0% Xylene
Shunway's stain for animal embryos. Used with Acid Fuchsin for staining Negri bodies. Stain Tech., 5, 34 (1928)
Counterstain to Hematoxylin for staining vaginal smears. Am. J. Anat., 61, 505 (1937).

RT

16910

25g

17.00

Technical Tip

When a stain is applied both en-bloc and as a post-stain for thin sections, maximum contrast will be achieved.

Indium Trichloride, Anhydrous

InCl₃   F.W. 221.18   CAS #10025-82-8
Chloride Content: 98%
A metal stain for nucleic acid
J. Biophys Biochem Cytol, 11,257 (1961)

42.00

RT

16920

5g

42.00

Ingrain Blue, C.I. #74240

Please see Alcian Blue 8GX

42.00

RT

10350

25g

200.00

Isopropyl Alcohol, Reagent, A.C.S.

(2-Propanol)
CH₃CH(OH)CH₃   F.W. 60.10   CAS #67-63-0
A.C.S. Specifications:

Color (APHA)	.	10
Density @ 25°C	.	0.781-0.783
Boiling Range (including 82.3°C)	.	1°C
Titratable Acid or Base	.	0.0001 meq/g
Residue after Evaporation	.	0.001%
Solubility in water	.	To pass test
Water	.	0.2%

RT	16930	1qt	12.00
RT	16931	4x1qt	42.00

JB-4(TM) Embedding Kit

Please see listing in Embedding Media Kits.

Karnovsky's Fixative

Please see Paraformaldehyde-Glutaraldehyde Solution.

Technical Tip

Elimination of Section Contamination During Staining

Kinyoun's Solution

Kinyoun's carbol fuchsin staining solution for acid-fast bacteria.

RT	26087-05	Kinyoun's Solution	500 ml	56.00	Add to Cart
RT	26087-06	Kinyoun's Solution	1 L	90.00	Add to Cart

Lanthanum Nitrate, Hexahydrate, Reagent

La(NO₃)₃·6H₂0   F.W. 433.03   CAS #10277-43-7

m.p.	..	40°C
Assay	..	Min 99.9%
Calcium	..	0.001%
Magnesium	..	0.0001%

A metal stain, intracellular tracer.

• Lane, N,J. and Treherne, J.E. (1970). Lanthanum staining of neurotubules in axons from cockroach ganglia. J. Cell Sci., 7:217
• Lane, N.J. (1972) Fine structure of a lipidopteran nervous system and its accessibility to peroxidase and lanthanum. Z. Zellforsch., 131:205

RT

17300

50g

28.00

Lead Acetate, Trihydrate Reagent, A.C.S.

Pb(CH₃COO)₂·3H₂0   F.W. 379.33   CAS #6080-56-4
A.C.S. Specifications:

Assay	.	99.0-103.0%
Chloride	.	5ppm
Copper	.	0.002%
Insoluble Matter	.	0.01%
Iron	.	0.001%
Nitrate and Nitrite (as NO3)	.	To pass test

0-5ºC	17600	100g		15.00
0-5ºC	17600-25	25g		5.00

*We are now offering Lead Acetate in 25ml bottles to avoid its classification as a hazardous chemical for shipping purposes.

Lead Citrate, Trihydrate

Pb(C₆H₅O₇)₂·3H₂O   FW 1053.82   CAS #512-26-5
Minimum assay 99.%
The most widely used metal stain for ultra thin sections.
Reynolds, E.S., J. Cell Bio., 17, 208 (1963)
Sato, T., J. Electron Micro., 116, 133 (1976)
Venable, J.H. and Coggeshall, R. (1965). A simplified lead citrate stain for use in electron microscopy. J. Cell Biol., 25:407
Famy, A. (1967). An extemporaneous lead citrate for electron microscopy. Proc. 25th Ann. EMSA Meet., p.148. Claitor's Pub. Division, Baton Rouge, LA.

RT	17800	25g	17.00
RT	17810	100g	52.00

Technical Tips

Handling of Lead Solutions
Lead Staining
Common Lead Stock Solutions
A Stable Lead Staining Solution

Lead Nitrate, Reagent, A.C.S.

Pb(NO₃)₂   F.W. 331.21   CAS #10099-74-8

Minimum Assay		99.5%
Insoluble matter		0.005%
Reaction		Not less than pH 3.5
Chloride		0.0005%
Calcium		0.01%
Copper		0.0005%
Iron		0.0005%
Potassium		0.002%
Sodium.		0.002%

A metal stain for ultra thin sections.
Sato, T., (1967) J. Electron Micro., 16, 733

RT

17900

25g

5.00

Light Green SF Yellowish, Certified, C.I. #42095

(Acid Green 5; Light Green 2G, S or 2GN; Acid Green F; Fast Acid Green N)
C₃₇H₃₄N₂Na₂O₉S₃   F.W. 792.86   CAS #5141-20-8
Solubility: 20.35% Water; 4.0% Alcohol; 5.25% Cellosolve; 12.0% Glycol; 0.0% Xylene

RT

17920

10 g

15.00

Light Green SF Yellowish Solution

Light SF Yellowish, 0.2% in 0.2% acetic acid fo use in the GM Method, as a fungal stain.

RT	26085-05	Light Green SF Yellowish, 0.2% in 0.2% Acetic Acid	500ml	39.00	Add to Cart
RT	26085-06	Light Green SF Yellowish, 0.2% in 0.2% Acetic Acid	1 L	65.00	Add to Cart
RT	26085-10	Light Green SF Yellowish, 1% Aqueous	500 ml	48.00	Add to Cart

(SPURR), Low Viscosity, Embedding Kit

Please see listing in Embedding Media Kits.

RT

14300

kit

60.00

Technical Tips

Embedding Resin Stain Permability

Lowicryl, Embedding Kit,

Please see listing in Embedding Media Kits

RT	14330	Lowicryl K4M	kit	352.00
RT	14340	Lowicryl HM20	kit	352.00
RT	14350	Lowicryl K11M	kit	352.00
RT	14360	Lowicryl HM23	kit	352.00

Lowicryl MonoStep Single Component Embedding Media

The same as our Lowicryl complete kits but this new formulation allows you one stop embedding without all of the cumbersome preparation and mixing.  This pre-mixed, ready to use resin, saves you time and minimizes chemical contact.  MonoStep Lowicryl is ideal for use in immunohistochemistry and immunolabeling.  Store at room temperature.

RT	14335	Lowicryl HM-20 Polar Embedding Medium; MonoStep	225g	155.00
RT	14345	Lowicryl HM-20 Polar Embedding Medium; MonoStep	225mg	155.00

LR White And LR Gold Resin

Please see listing in Embedding Media Kits.

RT	14370	LR Gold Complete	kit	212.00
RT	14380	LR White Complete	kit	116.00

Articles of Interest

Flat Embedding with LR-White

Lucifer Yellow, CH, Dilithium Salt

C₁₃H₉Li₂N₂O₉S₂   F.W. 457.25   CAS #67769-47-5

A technique producing an electron dense osmiophilic polymer when Lucifer Yellow is injected into cells. Lucifer Yellow also can be viewed in living cells as a dye marker and is more sensitive than any other dye.
Cell Tissue Res., 204, 37 (1979) J. Histochem Cystochem, 30, 189 (1982) Science, 217, 953 (1982); 215, 1641 (1982); 214, 801 (1981); 225, 851 (1984); Cell, 14, 741 (1978).
A fluorescent dye. Nature 292, 17 (1981)

0-5ºC

17950

25mg

96.00

Lugol’s Iodine

For use as a bacterial, fungal and inclusion body stain.

RT	26055-05	Lugol's Iodine Solution	500ml	21.00	Add to Cart
RT	26055-06	Lugol's Iodine Solution	1L	36.00	Add to Cart

Magnesium Chloride, Hexahydrate, Reagent, A.C.S.

MgCl₂·6H₂O   F.W. 203.30   CAS #7791-18-6
Assay: 99.0-102.0%

RT

18010

100g

20.00

Malachite Green 4, Certified

Please see Basic Green Oxalate

RT

18100

25g

38.00