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Electron Microscopy Sciences

Chemicals for Microscopy

arrow14Paraformaldehyde to Picric

msdsmsdsarrow12Paraformaldehyde, EM Grade, Purified

(Polyoxymethylene)
OH(CH2O)n H   F.W. Average: 600   CAS #30525-89-4
Paraformaldehyde is the condensation product of methylene glycol, a substance not so far isolated in its pure form; it is a white solid with a formaldehyde content between 78 and 98% and is supplied either as a Prill or Granular. All forms contain more than 95.5% paraformaldehyde content. The Prill and Granular forms are small, free-flowing, dust-free beads, the most desired by EM users. A fast-penetrating EM fixative used in combination with Glutaraldehyde, Acrolein, and Osmium Tetroxide. Karnovsky, M.J., J. Cell Biol., 27, 137A (1965). Hayat, M.A. Principles & Tech., of EM (Univ. Park Press), 68 (1981).

RT 19200 Prill 500g 14.00 Add to Cart
RT 19202 Prill kilo 21.00 Add to Cart
RT 19208 Granular 500g 13.00 Add to Cart
RT 19210 Granular kilo 20.00 Add to Cart

Technical Tip

Paraformaldehyde Static Jumps

Formaldehyde Solutionarrow12Paraformaldehyde Aqueous Solution -16% (Formaldehyde Aqueous Solution, 16%)

CAS #30525-89-4
Prepared from Paraformaldehyde crystal, 16% aqueous solution, methanol free. Microfiltered and stored in pre-scored ampoules.

RT 15700 16% Paraformaldehyde (formaldehyde) aqueous solution msds 10 ml 5.00 Add to Cart
RT 15710 16% Paraformaldehyde (formaldehyde) aqueous solution msds 10x10 ml 25.00 Add to Cart
RT 15710-S 16% Paraformaldehyde (formaldehyde) aqueous solution msds 100 ml bottle 24.00 Add to Cart
RT 15711 16% Paraformaldehyde (formaldehyde) aqueous solution msds 10 bxs/lot 235.00 Add to Cart
RT 15712 10% Paraformaldehyde (formaldehyde) aqueous solution msds 10x10 ml 24.00 Add to Cart
RT 15712-S 10% Paraformaldehyde (formaldehyde) aqueous solution msds 100 ml 23.00 Add to Cart
RT 15713 20% Paraformaldehyde (formaldehyde) aqueous solution msds 10x10 ml 29.00 Add to Cart
RT 15713-S 20% Paraformaldehyde (formaldehyde) aqueous solution msds 100 ml 27.00 Add to Cart
RT 15714 32% Paraformaldehyde (formaldehyde) aqueous solution msds 10x10 ml 34.00 Add to Cart
RT 15714-S 32% Paraformaldehyde (formaldehyde) aqueous solution msds 100 ml 33.00 Add to Cart

We can prepare in accordance to your special requirements (any concentration desired). Call for more information.

See Formaldehyde Solution, for other standard concentrations.

In addition to our complete line of Formaldehyde solutions shown above we have now added to the line.

Since the DOT (Department of Transportation) has changed the definition of what a non dangerous solution of formaldehyde is to under 10%, we are now offering an 8% and a 4% solution. This will allow us to ship overnight as well as overseas without any hazardous charges saving our customers a good deal of money.

The quality of our 8% and 4% aqueous Paraformaldehyde solution is as good as all of the other concentrations that we offer.

RT 157-4 4% Paraformaldehyde (formaldehyde) aqueous solution 10x10 ml 21.00 Add to Cart
RT 157-4-100 4% Paraformaldehyde (formaldehyde) aqueous solution 100 ml 20.00 Add to Cart
RT 157-8 8% Paraformaldehyde (formaldehyde) aqueous solution 10x10 ml 23.00 Add to Cart
RT 157-8-100 8% Paraformaldehyde (formaldehyde) aqueous solution 100 ml 22.00 Add to Cart

arrow12msdstechnical data sheetParaformaldehyde-Glutaraldehyde Solution Karnovsky's Fixative

Ready To mix. Kit consists of: 2x10 ml ampoules 16% Formaldehyde1x10 ml ampoules 50% Glutaraldehyde1x50 ml 0.2M Sodium Phosphate Buffer.

RT 15720   1 kit 20.00 Add to Cart
RT 15730   Lot of 5 kits 90.00 Add to Cart
Fixative Reagent Grade (Karnovsky’s)

This fixative contains 3% glutaraldehyde and 2% formaldehyde in 0.1M phosphate buffer, pH 7.4.

Karnovsky’s with formalin 2% and Biological glutaraldehyde 3% in 0.1m Phosphate Buffer.

RT 15731-10 Karnovsky Fixative Reagent Grade 1 L 248.00 Add to Cart
msdsKarnovsky’s with paraformaldehyde solution and EM grade glutaraldehyde

Please Note: This kit can be formulated to fit your requirements. Please contact us.

RT 15732-10 Karnovsky Fixative EM Grade 1 L 275.00 Add to Cart

arrow12Paramat and Paramat Extra

Please see listing in Embedding Media for Histology and Light Microscopy.

arrow12Tissue-TEK Paraffin

Please see listinginEmbedding Media.

arrow12Paraplast Embedding Medium

arrow12Paraplast Embedding MediummsdsParaplast X-tra

Handy pellet form melts rapidly. Melting Point 53-54°C. Cuts to 2 micron thickness with exceptional ribbon continuity. Extra compression resistance provides total support of tissues.

RT 19214 Paraplast X-tra 1 kilo 20.00 Add to Cart
RT 19215 Paraplast X-tra 8 kilo 122.00 Add to Cart
msdsarrow12Paraplast Plus

Reduces infiltration time by one-third. Cuts to 2 micron thickness with excellent ribbon continuity. Convenient pellet form melts rapidly. Melting point 56°C. Double filtered. No need for filtration before use.

RT 19216 Paraplast Plus 1 kilo 20.00 Add to Cart
RT 19217 Paraplast Plus 8 kilo 117.00 Add to Cart
msdsarrow12Paraplast Medium

Handy pellet form melts rapidly. Melting Point 56°C.
Cuts to 4 micron thickness with excellent ribbon continuity - no Crumbling or cracking.
Double filtered. No need for filtration before use.

RT 19218 Paraplast Medium 1 kilo 19.00 Add to Cart
RT 19219 Paraplast Medium 8 kilo 115.00 Add to Cart

arrow12Paraffin Waxes

For a continuation of our Paraffin Waxes, please see listing in Embedding Media.

Articles of Interest

Re-Embedding of Paraffin Embedded Specimens in Resin

arrow12Paraffin - Peel-A-Way® Embedding Wax

For our complete line of Embedding Waxes, please see Embedding Media for Histology and Light Microscopy

arrow12PEG - GMA/PEG Embedding Kit,

Please see listing in Embedding Media Kits.

msdsarrow12Parlodion (Pyroxylin Purified Strips)Parlodion (Pyroxylin Purified Strips)

C12H16N4O18   F.W. 504.28   CAS #9004-70-0
Highly purified from cellulose nitrate; specially prepared for embedding tissue for sectioning and for preparing semi-permeable membranes.

RT 19220   10g 170.00 Add to Cart

arrow12Parlodion 2% in Amyl Acetate

See Collodion, 2%.

arrow12Pepsin Reagent, Ready To Use

Description: Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Pepsin digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens.
Storage: 2-8°C. DO NOT FREEZE
Intended Use: Use as antigen retriever for some antibodies in IHC. Please refer to primary antibody protocol for IHC.
Reagent: Ready-to-use Pepsin reagent available in 2 sizes: 15 and 100 ml
4-8°C 64142-05 Pepsin Reagent 15 ml 75.00 Add to Cart
4-8°C 64142-06 Pepsin Reagent 100 ml 145.00 Add to Cart

msdsarrow12Periodic Acid, Reagent

H5IO6   F.W. 227.96   CAS #10450-60-9

Assay   >99.0%
Heavy Metals   .0.005%
Insoluble Matters   0.01%
Iron   .0.003%
Other Halogen (Cl)   0.01%
Residue after Ignition   0.01%
Sulfate   0.01%

Used as a Fixative: Issidorides, M.R., and Kasorchis, T. (1981).
Dispersed and compact chromatin demonstrated with a new EM Method: phosphotungstic acid-hematoxylin block-staining. Histochemistry 73,21.
Used widely as a Negative Stain:

  • As Spray method: Horne, R.W., and Pasquali-Ronchette, I. (1974). A negative staining-carbon film technique for studying viruses in the electron microscope.
  • Preparation procedures for examining icosahedral and filamentous viruses. J. Ultrastruct. Res. 47, 361. Locke, M., and Kirhman, N. (1971).
  • Hot Alcoholic phosphotungstic acid and uranyl acetate as routine stains for thick and thin sections. J. Cell Biol., 50, 550., Farragiana, T., and Marinozzi, V. (1979).
  • Phosphotungstic acid staining of polysaccharides containing structures on epoxy embedded tissues. J. Submicrosc. Cytol. 11, 263.Bloom, E.E., And Aghajanian, G.K. (1968).
  • Fine structural and cytochemical analysis of the staining of synaptic junctions with PTA. J. Ultrastruct. Res. 22, 261.
RT 19325   25 g 32.00 Add to Cart

arrow12Periodic Acid Solutions

Please see listing in Reagents for Histology

arrow12Permount™ Mounting Medium

Please see listing in Adhesives and Mountants

msdsarrow12Phenol Red

(Phenolsulfonphthalein)
C19H14O5S   F.W. 354.38   CAS #143-74-8
A standard indicator for preparing buffers, pH range 6.4-8.2.

RT 19327   5 g 17.00 Add to Cart

msdsarrow12Phenol Red Sodium Salt

(Phenolsulfonphthalein Sodium Salt)
C19H13NaO5S   F.W. 376.37    CAS #34487-61-1
A standard indicator for preparing buffers, pH range 6.8-8.2.

Assay   >99.0%
Water   <0.1%
RT 19328   5 g 26.00 Add to Cart

msdsarrow12 (PMSF), Phenylmethylsulphonyl Fluoride

C6H5CH2SO2F   F.W. 174.20   CAS #329-98-6

Assay   >99.0%
Water   <0.1%
RT 19330   1g 19.00 Add to Cart
RT 19331   5g 90.00 Add to Cart

msdsarrow12Phosphate Mixed Sodium Salts 0.2 M Buffer

Please see listing in Prepared Buffers.

RT 11600-05   500 ml 19.00 Add to Cart
RT 11600-10   1 L 28.00 Add to Cart

msdsarrow12Phloxine B, Certified, C.N. #DcPh-6

(Acid Red 92; Cyanosine; Eosin 10B; Phloxine TA, N, BP Super, RB, TB, or BB)
C20H2O5Cl4Br4Na2   F.W. 829.66   CAS #18472-87-2
Solubility: 10.5% Water; 5.0% Alcohol; 9.0% Cellosolve; 4.5% Glycol; 0.0% Xylene

RT 19350   25 g 36.00 Add to Cart

msdsarrow12Phosphomolybdic Acid, Crystal, Reagent, A.C.S.

(dodeca-Molybdophosphoric Acid)
H3PO4·12(MoO3)·24H2O   F.W. 2257.6   CAS #51429-74-4

RT 19400   100g 85.00 Add to Cart

arrow12Phosphomolbdic Acid Solutions

Please see listing in Reagents for Histology

msdsarrow12Phosphotungstic Acid, Crystal, Reagent, Highest Purity

(Tungstophosphoric Acid)
H3[P(W3O10)4]   aq F.W. 2880.17+aq   CAS #12501-23-4
Specifications:

Solubility   Practically clear,
colorless solution.
Ammonia (NH )   0.004%
Chloride (Cl)   0.03%
Insoluble Matter   0.02%
Nitrate (NO3 )   0.004%
Sulfate (SO4 )   0.005%
Heavy Metals and Iron.   No color formation on
addition of Hydrogen Sulfide

Used as a Fixative:

Issidorides, M.R., and Kasorchis, T. (1981).
Dispersed and compact chromatin demonstrated with a new EM Method: phosphotungstic acid-hematoxylin block-staining. Histochemistry 73,21.

Used widely as a Negative Stain:

  • As Spray method: Horne, R.W., and Pasquali-Ronchette, I. (1974). A negative staining-carbon film technique for studying viruses in the electron microscope.
  • Preparation procedures for examining icosahedral and filamentous viruses. J. Ultrastruct. Res. 47, 361. Locke, M., and Kirhman, N. (1971).
  • Hot Alcoholic phosphotungstic acid and uranyl acetate as routine stains for thick and thin sections. J. Cell Biol., 50, 550.,Farragiana, T., and Marinozzi, V. (1979).
  • Phosphotungstic acid staining of polysaccharides containing structures on epoxy embedded tissues. J. Submicrosc. Cytol. 11, 263.Bloom, E.E., And Aghajanian, G.K. (1968).
  • Fine structural and cytochemical analysis of the staining of synaptic junctions with PTA. J. Ultrastruct. Res. 22, 261.
RT 19500   100g 54.00 Add to Cart

arrow12Phosphotungstic Acid Solutions

Please see listing in Reagents for Histology.

msdsarrow12 Picric Acid, Reagent, Crystal, A.C.S.

(2,4,6-Trinitrophenol)
C6H2(NO2) 3OH   FW 229.11   CAS #88-89-1
A.C.S. Specifications:

Water   min 30%
Melting Point (dried)   121-123°C
Insoluble in Resinous Matter.   0.01%
Insoluble in benzene   0.1%
Sulfate   0.01%
RT 19550   100g 90.00 Add to Cart

arrow12Picric Acid Solutions

Please see listing in Reagents for Histology.

Pipes to Ruthenium arrow12