Pipes Buffer Solution

Please see listing in Prepared Buffers

RT

11610

500ml

23.00

Pipes, 1.5 Sodium Salt, Crystal

[Piperazine-N,N-bis (2-ethanesulfonic acid,) 1.5 Sodium]
F.W. 335.3   pK = pH = 6.8 @25°C    CAS #10010-67-0

RT	19230		25g	21.00
RT	19240		100g	64.00

Plastisolve

Please see Methylene Chloride .

RT	19600		1qt	19.50
RT	19610		4x1qt	74.00

Poly-L-Lysine Solutions

A/ Poly-L-Lysine Hydrobromide 30,000-70,000

CAS #25988-63-0

A polycation, which binds to DNA, red cell membranes and any negatively, charged proteins. It is useful for promoting cell adhesion; for the preparation of polycationic beads, useful in immobilization techniques; immobilization of membranes; immobilization of plant protoplasts. In cell culture, immobilization by micro encapsulation with the alginate-poly-L-lysine microcapsule system We offer a prepared 0.1% aqueous solution, micro-filtered, which is ideal for cell culture growth.

RT

19320-A

Poly-L-lysine 0.1% Solution

10ml

28.50

B/ Poly-L-Lysine Hydrobromide 70,000-150,000

CAS #25988-63-0

Poly-L-lysine with a molecular weight of >70,000. This solution is useful in promoting cell adhesion to solid substrates. Used for nuclear antigen-coated red cells in hymolytic plaque assay.

We offer a prepared 0.1% aqueous solution which is micro-filtered and is used to increase tissue adhesion to the glass slide, which is needed for immunohistochemical and immunolabeling techniques, as well as lengthy staining procedures.

RT

19320-B

Poly-L-lysine 0.1% Solution

10ml

7.75

Polyester wax

A synthetic polyester wax with a very low melting point (37°C). Invented by Dr. H.F. Steedman, as a ribboning embedding medium. Its main advantage over paraffin and ester waxes is its low melting point, which reduces tissue hardening, shrinkage, and eliminates the need for infiltration ovens.

The wax is soluble in most organic solvents, including alcohol, ethers, esters, ketones and hydrocarbons. The wax is water tolerant, almost opaque, and sections easily. No electrification of ribbons occurs during sectioning. Sections of 2 microns and more may be cut at room temperatures between 10 and 22°C. It is advantageous to keep the wax in its solid state prior to use.

RT

19312

Polyester Wax

500g

125.00

Polyethylene Glycol, Carbowax

Please see Carbowax.

Polyvinyl Alcohol 15000

(CH₂CHOH)_n   F.W. 14000-15000   CAS #9002-89-5
Degree of Hydrolyzation: 86-89 mol%

• A water-miscible embedding medium: Munoz-Guerra,S. and Subrirana, J.A. (1982).
• A crosslinked polyvinyl alcohol: a water-miscible polymer used as an embedding medium for electron microscopy. Mikroskopie, 39:346

RT

19800

500g

65.00

Ponceau B.S.

See Biebrich Scarlet

Potassium Bromide, Reagent, A.C.S.

KBr   F.W. 119.00   CAS #7758-02-3
Specifications:

Insoluble Matters	0.005%
Bromate	To Pass Test
Chloride	.0.2%
Iodide	0.001%
Nitrogen	0.005%
Sulfate	0.005%
Barium	0.002%
Heavy Metals	0.0005%
Sodium	0.02%

Used in the preparation for autoradiography

RT

20040

100g

18.00

Potassium Chloride, Crystal, Reagent, A.C.S.

KCl   F.W. 74.55   CAS #7447-40-7 
Specifications:

Insoluble Matters	0.005%
Iodide	0.002%
Bromide	0.01%
Chlorate and Nitrate	0.003%
Sulfate.	0.001%
Phosphate	0.0005%
Sodium	0.005%

RT

20060

100g

11.00

Potassium Dichromate, Crystal, Reagent, A.C.S.

K₂Cr₂O₇   F.W. 294.18   CAS #7778-50-9
A.C.S. Specifications:

Insoluble and NH4OH ppt.	0.005%
Chloride	0.001%
Sulfate	0.005%
Calcium	0.003%
Sodium	0.02%
Loss on drying at 105°C	0.05%

RT

20100

250g

21.50

Potassium Dichromate Solutions

Please see listing in Reagents for Histology.

Potassium Ferricyanide, Reagent, A.C.S.

K₃Fe(CN)₆   F.W. 329.25   CAS #13746-66-2
A.C.S. Specifications:

Chloride	0.01%
Ferro Compounds (Fe(Cn)4)	0.05%
Insoluble Matters.	0.005%
Sulfate	0.01%

RT

20150

100g

18.00

Potassium Hydroxide, Pellets, Reagent, ACS.

KOH   F.W. 56.11   CAS #1310-58-3
Assay>85.0%
Water contains: 10-15%

RT

20155

100g

10.00

Potassium Iodide, Granular, Reagent, A.C.S.

KI   F.W. 166.00   CAS #7681-11-0
Refer to Bismuth Carbonate for references on use.

RT

20158

50g

18.00

PotassiumOxalate, Reagent, A.C.S.

(CO₂K)₂·H₂O   F.W. 184.23   CAS #6487-48-5
Assay: 98.5-101.0%
Neutrality: To Pass Test

Specifications:
Insoluble Matters	0.01%
Chloride	0.002%
Sulfate	0.01%
Ammonium	0.002%
Heavy Metal	0.0025%
Iron	0.001%
Sodium	.0.02%

RT	20160		100g	16.50
RT	20162		500g	78.00

Potassium Permanganate, Reagent, A.C.S.

KMnO₄   F.W. 158.03   CAS #7722-64-7
A.C.S. Specifications:

Chloride & Chlorate	0.005%
Nitrogen Compounds	0.005%
Sulfate	.0.02%
Insoluble Matters	0.2%

An EM fixative and a metal stain. J. Ultrastruct. Res., 21,424 (1968)

RT

20200

250g

34.00

Potassium Permanganate Solutions

Please see listing in Reagents for Histology.

Potassium Phosphate, Monobasic, Reagent, A.C.S.

KH₂PO₄   F.W. 136.09   CAS #7778-77-0
A.C.S. Specifications:

Insls. and Calcium, and NH4OH ppt	0.01%
Loss on drying over H2SO4	0.2%
pH of 5% Solution @ 25°C	4.1-4.5
Chloride	0.001%
Nitrogen Compounds	0.001%
Sulfate	0.003%
Sodium	0.005%
Iron	0.002%

RT

20250

500g

32.00

Potassium Pyroantimonate, Trihydrate Potassium antimonate

KSbO₃·3H₂O   F.W. 262.90   CAS #12208-13-8
Minimum assay: 94%
Minimum sensitivity to Na: 1:430
Insoluble matternot more than 0.01%
*Ultrastructural localization sodium. J. Histochem Cystochem., 24, 740 (1976)

RT

20220

25g

18.00

Potassium Sodium Tartrate, Tetrahydrate Reagent, A.C.S.

(Sodium Potassium Tartrate)
KNaC₄H₄O₆·4H₂O   F.W. 282.22   CAS #6381-59-5
Assay 99.0-102.0%
Water 21.0-27.0%
Used with bismuth subnitrate for staining polysaccharides, including glycogen without oxidizing agents.

• Shinji, Y., et al (1975). A new electron microscopic histo-cyto chemical staining method: demonstration of glycogen particles. Acta Histochem., Cytochem., 8:139

RT

20260

100g

11.00

2-Propanol, Propyl Alcohol

Please see Iso-propyl Alcohol.

Propylene Glycol, Reagent, A.C.S.

(1,2-Propanediol)
CH₃CHOHCH₂OH   F.W. 76.10   CAS #57-55-6
Specific Gravity (H₂O=1): 1.04
Assay>99.5%

Specifications:

Residue after Ignition	0.005%
Chloride	1ppm
Water	0.2%
Titratable Acid	0.0005meq/g

RT	20350		450ml	27.00
RT	20352		4x450ml	94.00

Propylene Oxide, EM Grade

(1,2-Epoxypropane, Methyloxirane)
CH₃CHCH₂O   F.W. 58.08   CAS #75-56-9
m.p. -112°C; b.p. 34°C; f.p. -37°C
Vapor pressure @ 33°C: 980 mbar
Specifications:

Specific Gravity @ H2O=1	.0.831
Acidity, Wt. ppm as Acetic Acid	2
Aldehydes, ppm as Propionaldehyde	3
Water,wt. ppm	35
Total Chloride, wt. ppm as Cl	1
Nonvolatile matter, g/100ml	max 0.001
Color, Platinum-Cobalt	.0

A solvent agent for epoxy resins. Used widely for infiltration and gradual replacement of the dehydration agent.

RT	20401	Metal can	500ml	18.00
RT	20411	Metal can	4x500ml	64.00
RT	20412	Metal can	250ml	16.00
RT	20414	Metal can	4x250ml	56.00

Pyronin Y, Certified, C.I. #45005

(Pyronin G)
C₁₇H₁₉N₂OCl  F.W. 302.81  CAS #92-32-0
Solubility: 9.0% Water; 0.5% Alcohol; 1.85% Cellosolve; 4.35% Glycol; 0.0% Xylene

RT

19560

5 g

36.00

Quetol 651, Resin

(Ethylene Glycol Diglycidyl Ether)
C₈H₁₄O₄   F.W. 174.20   CAS #2224-15-9
Specific Gravity (H₂O=1) : 1.15

0-5ºC

20440

225ml

62.00

Quetol 651, NSA Kit

Please see listing in Embedding Media Kits.

0-5ºC

14640

kit

74.00

Quinolinic Phthalocyanine

Please see Cuprolinic Blue.

RT	12800		0.1gm	184.00
RT	12802		0.2gm	366.00

Replicating Materials

Please see the complete listing in the Materials Science section.

Ruthenium Red, 37.5% Ru

[(NH₃)₅RuORu(NH₃)₄ORu(NH₃)₅]Cl₆·4H₂O   F.W.858.42   CAS #11103-72-3

• Stain for monopolysaccharides and tight junctions. Luft, J.T., Proc. 6th Int. Cong. Em (1966); J. Cell. Biol., 57, 874 (1973).
• Stain for pectin and for diverse cell surfaces. J. Cell Biol.,23, 54A (1964).
• "Positive Staining for EM" van Nostrand Reinhold Co., New York, NY (1975) pp 163-165.
• Ruthenium Red and Violet II. Fine structural localization in animal tissues. Luft J.H. Anat. Rec., 171:369, (1971a)
• Structure and staining characteristics of myofiber external lamina. Zacks, S.I. et al. J. Histochem. Cytochem., 21:703 (1973)
• Glycoaminoglycans in developing chick-embryo aorta revealed by ruthenium red: an electron microscopy study. Kadar, A. et al. J. Pathol., 108:275 (1973)
• Uptake of marker particles by in vitro ventilated and perfused rat lung. Vidic, B. Am. J. Anat., 138:521 (1973)

RT

20600

1g

84.00

Ruthenium Tetroxide, 0.5% Stabilized Aqueous Solution

In Crystal form: RuO₄   F.W. 165.70   CAS #20427-56-96
m.p. 25.4°C b.p. 40°C
Ruthenium tetroxide is very similar to Osmium tetroxide, and is used as a fixation/staining agent for electron microscopy. Ruthenium tetroxide offers excellent staining of saturated and unsaturated polymer materials as well as stabilizing thin sections under the bombardment of the electron beam.
NOTE: Penetration of ruthenium tetroxide into the tissue is poor.

• Caughey, R.C. and Miller, M.A., Proceed. of 44th Ann. Mtg. EMSA (1986), page 256.
• Hayat, M.A., Basic Technique for Electron Microscopy, Academic Press, NY, 1986, p 22.
• Trent, J.S., Macromolecules, 17, 2930 (1984); 16, 539 (1983).

RT

20700-05

Box 5x10 ml

128.00

Safranin to Sym-Collidine