Safranin O, Certified, C.I. #50240

(Safranin T, Y or A; Basis Red 2; Cotton Red; Gossypimine
C₂₀H₁₉N₄Cl   F.W. 350.85   CAS #477-73-6
Solubility: 4.5% Water; 3.5% Alcohol; 5.0% Cellosolve; 3.5% Glycol; 0.0% Xylene

RT

20800

25 g

27.00

Schiff’s Reagent

For use in the Periodic Acid Leucofuchsin (PAS) method as a general tissue stain.

0-5°C	26052-05	Schiff’s Reagent	500ml	36.00	Add to Cart
0-5°C	26052-06	Schiff’s Reagent	1L	62.00	Add to Cart

Scott’s Tap Water Substitute

Magnesium sulfate buffered with sodium bicarbonate. Readyto- use.

RT	26070-06	Scott’s Tap Water Substitute	1 L	30.00	Add to Cart
RT	26070-07	Scott’s Tap Water Substitute	4 L	95.00	Add to Cart

Silver, Collodial

Please see our complete listing.

Silver Lactate, Powder

CH₃CH(OH)CO₂Ag   F.W. 196.94  CAS #128-00-7
Silver content: approx. 90% min.
* To localize intracellular anions (Cl-). Komnick, (1962).
* Elektronemikrokopische Lokalisation von Na+ und Cl in Zellan und Geweban. Protoplasma, 55,414.

RT

21000

5g

35.00

Silver Protein, Strong

Protargol, 8.3% Ag  CAS #9008-42-8
Thiery, (1967). See Silver Nitrate

RT

21100

5g

87.50

Silver Nitrate, Reagent, A.C.S.

AgNO₃   F.W. 169.89  CAS #7761-88-8
Assay 99.9+%
A.C.S. Specifications:

Substances not precipitated by HCl-max	0.010%
Sulfate max	0.0002%
Copper max	0.0002%
Iron max	0.0002%
Lead max	0.001%

References for Silver stain:

• Swift, J.A. (1968). The electron histochemistry of cystine-containing proteins in thin transverse sections of human hair. J.R. Microsc. Soc. 88,449.
• Thiery, J.P. (1967). Mise en evidence des Polysaccharides sur coupes fines en microscopie eletronique. J. Microsc. (Paris) 6,987.
• Rambourg, A. (1967). An improved silver methenamine technique for the detection of periodic acid-reactive complex carbohydrates with the electron microscope.J. Histochem. Cytochem. 15, 409.
• Hermandez et al., (1968)). Periodic acid-chromic acid-methenamine silver technique for glycoprotein detection in the electron microscope. J. Histochem. Cystochem. 16, 507.
• Ribi, W.A. (1976). A golgi-electron microscope method for insect nervous tissue. Stain Technol. 51,13.
• Braak, H., and Braak, E. (1982). A simple procedure for electron microscopy of Golgi-impregnated nerve cells. Neurosci. Lett. 32,1.

RT	21050		25g	45.00
RT	21052		100g	180.00

Silver Nitrate Solutions

Please see listing in Reagents for Histology

(S-TPPS) Silver Tetraphenylporphin Sulfonate

An EM stain for elastin. Albert, E.N., And Fleischer E. (1970).
A new electron dense stain for elastic tissue. J. Histochem.Cystochem. 18, 697

0-5ºC

21110

100mg

50.00

Sodium Acetate,Trihydrate Reagent, A.C.S.

CH₃COONa·3H₂O   F.W. 136.08   CAS #6131-90-4
A.C.S. Specifications:

Assay	99.0-100.5%
Ca, Mg and R2O3 ppt	0.01%
Chloride	0.001%
Insoluble Matter-	0.005%
Iron, Heavy metal, Phosphate	5ppm/ea
Sulfate	0.002%
Potassium	0.005%
pH of 5% sol. @ 25°C	7.5-9.2

RT

21120

500g

48.00

Sodium Bicarbonate, Powder, Reagent, A.C.S.

NaHCO₃   F.W. 84.01   CAS #144-55-8
Assay 97.7-100.3%
Specifications:

Insoluble Matters	.0.015%
Chloride	0.003%
Phosphate	0.001%
Sulfur Compounds	0.003%
Ammonium	5ppm
Iron	0.001%
Potassium	0.05%

RT

21125

500g

21.00

Sodium Bisulfite Solution

A 1% aqueous stock solution for fungal staining.

RT	26084-05	Sodium Bisulfite Solution	500ml	15.00	Add to Cart
RT	26084-06	Sodium Bisulfite Solution	1 L	23.00	Add to Cart

Sodium Borate, Tetra, Granular, Reagent, A.C.S

Please see Borax Tetra.

Sodium Cacodylate Trihydrate Sodium dimethyl arsenate,

Please see Cacodylic Acid, Sodium Salt .

RT	12300		100g	80.00	Add to Cart
RT	12310		500g	370.00	Add to Cart

Sodium Cacodylate, Buffer Solution

Please see listing in Prepared Buffers.

RT	11650		225ml	22.00	Add to Cart
RT	11652		500ml	34.00	Add to Cart

Sodium Carbonate, Anhydrous, Reagent, A.C.S.

Na₂CO₃   F.W. 105.99   CAS #497-19-8
Specifications:

Insoluble Matters	0.01%
Loss on Heating at 280°C	1.0%
Chloride	0.001%
Phosphate.	0.001%
Nitrogen Compounds	0.001%
Sulfur Compounds.	0.003%
Ammonium Hydroxide ppt.	0.01%
Arsenic.	1ppm
Ca and Mg	0.01%
Heavy Metals	5ppm
Iron	5ppm
Potassium	0.005%
Silica (SiO2)	0.005%

RT

21135

500g

33.00

Sodium Citrate, Granular, Reagent

Na₃C₆H₅O₇·2H₂O   F.W. 294.11   CAS #6132-04-3

Assay	99.9%
Insoluble Matters	0.005%
Free Acid (as Citric)	0.02%
Free Alkali	0.05%
Chloride	0.003%
Sulfate	0.005%
Calcium	0.005%
Heavy Metals	.3ppm
Iron	0.001%

RT

21140

500g

14.00

Sodium Ethylate

(Sodium Ethoxide)
C₂H₅ONa   F.W. 68.05   CAS #141-52-6
Used as an agent for flattening thick sections (0.5-2.0 micron) of epoxy embedded material. Felix Sierra, Christa Hegele-Hartung and Henning M. Beier (1990).
An improved technique for flattening semithin sections of epoxy-embedded tissues used for high resolution light microscopy. J. of Microscopy Vol 159, Pt 1, (July 1990), pp. 95-98

RT

21150

25g

10.00

Technical Tips

An Improved Technique for Flattening Semithin Sections of Epoxy-Embedded Tissues Used for High-Resolution Light Microscopy

Sodium Hydroxide, Pellets, Reagent, A.C.S.

NaOH   F.W.40.00   CAS #1310-73-2
Assay >97.0%
Specifications:

Sodium Carbonate	1%
Nitrogen Compounds	0.001%
Chloride	0.005%
Phosphate	0.001%
Sulfate	0.003%
Heavy Metals	0.002%
Ammonium Hydroxide ppt	0.02%
Iron	0.001%
Potassium	0.02%
Nickel	0.001%
Mercury	0.1ppm

RT	21160		100g	7.50
RT	21162		500g	19.00

Sodium Hydroxide, Solution

2N Standardized   CAS #1310-73-2
Carbonate-free. This solution is prepared from ACS Reagent grade sodium hydroxide, and is standardized colormetrically against potassium biphthalate primary reference material.
Normal Concentration 1.98-2.02

RT	21170	Sodium Hydroxide 2N Solution	225ml	12.00
RT	21170-01	Sodium Hydroxide 1N Solution	225ml	10.00

Sodium Methoxide, Anhydrous

(Sodium Methylate)
CH₃ONa   F.W. 54.03   CAS #124-41-4
Used in the removal of resin from sections.

RT

21200

20g

12.00

Sodium Phosphate, Dibasic, Heptahydrate, Reagent, A.C.S.

Na₂HPO₄·7H₂O   F.W. 268.07   CAS #7782-85-6

Assay	98.0-102.0%
Loss on Drying @ 105°C	43-50%
pH of a 5% solution @25°C	8.7-9.3
Specifications:
Insoluble Matters	<0.005%
Chloride	0.001%
Nitrogen Compounds	0.001%
Sulfate	0.005%
Arsenic	0.0005%
Heavy Metals	0.001%
Iron	0.001%

RT	21180		500g	26.00
RT	21182		1 kg	50.00

Sodium Phosphate, Monobasic,

Hydrate,Reagent, A.C.S.
NaH₂PO₄·H₂O   F.W. 137.99   CAS #10049-21-5
Assay 98.0-102%
pH of a 5% solution @25ºC-4.1-4.5
Specifications:

Insoluble Matters-	<0.01%
Chloride	5ppm
Nitrogen Compounds	0.001%
Sulfate.	0.003%
Arsenic.	0.5ppm
Heavy Metals	0.001%
Iron	0.001%

RT	21190		500g	30.00
RT	21192		1 kg	56.00

Technical Tips

Temperature Conversions

Sodium Sulfite, Reagent, A.C.S.

Na₂SO₃   F.W. 126.04   CAS #7757-83-7
Assay >98.0%
Specifications:

Insoluble Matters	0.005%
Free Acid	To Pass Test
Titratable Free Base	0.03meq/g
Chloride	.0.02%
Arsenic	0.0001%
Heavy Metals	0.001%
Iron	0.001%

RT

21300

1 kg

26.00

Technical Tip:

Improving the Adhesion of Sections Filmless Grids

Sodium Tartrate, Reagent, A.C.S.

Na₂C₄H₄O₆·2H₂O   F.W. 230.08   CAS #6106-24-7
Assay 99.5%-101.0%
pH of a 5% solution @25ºC-7.0-9.0
Loss on Drying @150ºC-15.61-15.71%
Specifications:

Insoluble Matters	0.005%
Chloride	0.0005%
Phosphate	0.0005%
Sulfate	0.005%
Ammonium	0.003%
Calcium	0.01%
Heavy Metals	0.0005%
Iron	0.001%

RT

21350

25 g

12.50

Sodium Tungstate, Reagent, A.C.S.

Na₂WO₄.2H₂O   FW 329.86   CAS #10213-10-2
Assay 99.0-101.0%
Specifications:

Insoluble Matters	0.01%
Chloride	0.005%
Molybdenum	0.001%
Nitrogen	0.001%
Sulfate	0.01%
Arsenic	0.0005%
Heavy Metals	0.001%

• Stockert,J.C. (1977). Sodium Tungstate as a stain in electron microscopy. Biol. Cellul., 29:211
• Takeuchi, I.K. (1981). Differential staining of nucleoli and chromatin by sodium tungstate. J. Electron Microsc., 30:15

RT

21400

25g

13.50

Sorensen's Phosphate Buffer

Please see listing in Prepared Buffers

0-5ºC	11682-05		500ml	18.00
0-5ºC	11682-10		1L	26.00

(SPURR), Low Viscosity Embedding Kit

Please see listing in Embedding Media Kits.

RT

14300

kit

60.00

Staining Solutions

We now offer a complete line of prepared, ready-to-use, high quality staining solutions for all standard staining procedures used by the Biological Staining Commission and the Armed Forces Institute of Pathology.

• All solutions and concentrations are packaged and prepared in convenient and precise quantities
• Premixed ready-to-use; saves you time and effort
• Consistently uniform results are obtained.

For a complete list and description of all of our stains, please see Chemicals for Microscopy

For Prepared Staining Kits, please see the complete listing of Prepared Staining Solutions

Technical Tips

Improving the Adhesion of Sections Filmless Grids

Styrene, Reagent

(Vinylbenzene)
C₆H₅CH:CH₂   F.W. 104.14   CAS #100-42-5
Specific Gravity (H₂O=1): 0.909
Assay >99.0%
Stabilizer (p-tert-Butylcatechol)-10-15ppm
A monomeric styrene used as an embedding media for ultramicrotomy.

• DeLamater, E.D., et al (1971). The use of styrenes as embedding media for electron microscopy. Pro. 29th Ann. Meet. EMSA, p. 488. Claitor's Pub. Divsion, Baton Rouge, LA
• Kushida, H. (1962b). On ultraviolet polymerization of styrene resins in embedding for electron microscopy. J. Electron Microsc., Vol. 2. p.10. Academic Press, New York.
• Kushida, H. (1961a). A styrene-methacrylate resin embedding method for ultrathin sectioning. J. Electron Micro., 10:16

RT

21500

450ml

10.00

Styrene Embedding Kit

Please see listing in Embedding Media Kits.

RT

14650

kit

38.00

Succinic Acid, Crystal, Reagent, A.C.S.

(CH₃COOH)₂   F.W. 118.09   CAS #110-15-6
m.p. 185-191°C
Assay >99.0%
Specifications:

Insoluble Matters-	.0.01%
Chloride	0.001%
Sulfate	0.003%
Phosphate	0.001%
Nitrogen Compound	0.001%
Heavy Metals	5ppm
Iron	5ppm

RT

21550

100g

16.00

Sucrose, Reagent, A.C.S.

C₁₂H₂₂O₁₁   F.W. 342.20   CAS #57-50-1
Specifications:

Insoluble Matters	0.005%
Loss on Drying 105ºC	0.03%
Residue after Ignition	0.01%
Titratable acid	0.0008meq/g
Chloride	0.005%
Sulfate	0.005%
Heavy Metals-	5ppm
Iron	5ppm
Invert Sugar	0.05%

RT

21600

500g

19.95

Sudan Black B, Certified, C.I. #26150

(Solvent Black 3)
C₂₉H₂₄N₆   M.W. 456.56   CAS #4197-25-5
Solubility: Water-insoluble; Alcohol-soluble; 4.0% Cellosolve; 1.0% Glycol.

RT

21610

25 g

28.00

Sure-cut Surfactant

Epoxy resins are easier to section with glass, as well as diamond knives, when the embedding medium contains 0.5-1.0% of our SURE-CUT. The life of the diamond knife will be extended with its use because it will reduce the friction of the plastic block during sectioning.

RT

21630

50g

26.00

Sym-Collidine, Buffer kit

Please see listing in Prepared Buffers.

RT	11500		one set	20.00
RT	11510		box/5 sets	75.00

Sym-Collidine, Reagent

Please see listing in Prepared Buffers.

RT

11520

Sym-Collidine

100ml

84.00

Tannic Acid to Z6040