Tannic Acid, Reagent, A.C.S., EM Grade

C₇₆H₅₂O₄₆   F.W. 1701.28   CAS #1401-55-4

RT

21700

100 g

13.00

Tannic Acid, Reagent, A.C.S.

Gallotannin; Chiefly   (C₁₄H₁₀O₉)n   F.W. range 1000.00-1500.00  CAS #1401-55-4
Low Molecular Weight. Suitable for electron microscopy

• Takagi, M., Parmley, R.T., Denys, F.R. and Kageyama, M. (1983). Ultrastructural visualization of complex carbohydrates in epiphyseal cartilage with the tannic acid-metal salt methods. J. Histochem. Cytochem.,31:783
• Simionescu, N. el al., J. Cell Biol., 70:608 (1976) A Combination of Tannic acid Glutaraldehyde Osmium Tetroxide (TAGO) Method. improving membranes contrast.
• Roubos, E. W. and Van DerWal-Divendel, R.M. (1980). Ultrastructural analysis of peptide-hormone released by exocytosis. Cell Tiss. Res., 207:267
• Buma, P.,Roubos, E.W. and Buijs, R.M. (1984). Ultrastructural demonstration of exocytosis of neural, neuroendocrine, and endocrine secretions with an in vitro tannic acid (TARI) method. Histochemistry, 80:247
• Buma, P. and Nieuwenhuys, R. (1987). Ultrastructural demonstration of oxytocin and vascopressin release sites in the neural lobe and median eminence of the rat by tannic acid and immunogold methods. Neurosci. Lett., 74:151
• Brooks, J.C. and Carmicheal, S.W. (1987). Ultrastructural demonstration of exocytosis in intact and saponin-permeabilized cultured bovine chromaffin cells. Am. J. Anat., 178:85

RT

21710

100g

46.00

Tannic Acid Solutions

Please see listing in Reagents for Histology.

Technovit: Glycol Methacrylate Embedding Kits

Please see listing in Embedding Media Kits .

TMB, 3,3'5,5'-Tetramethylbenzidine

C₁₆H₂₀N₂   F.W.240.35   CAS #54827-17-7
m.p. 166-169°C
Assay >98%
A non-carcinogenic substitute for DAB and DAB-HCl. Used as a sensitive and specific reagent for the detection of blood.

• Standefer, J.C., Vanderjagt, D. (1977) Assay of Hemoglobin. Clin. Chem. 23, 749.
• Boss, E.S., et al., (1981). Assay of Peroxidases. J. of Immunoassay. 2,187.
• Holland, V.R., et al., (1974) Tetrahedron, 30, 3299.

0-5ºC

13070

1g

27.00

TMB-HCl, Dihydrochloride TMB

C₁₆H₂₀N₂·2HCl   F.W. 313.30   CAS #64285-73-0
m.p. >300°C
Assay >98% (Cl)

0-5ºC

13074

TMB-HCl

1gm

42.00

Also available in tablet form. 1mg/ tablet, ready to use.

0-5ºC	13075	TMB-HCl tablets	10 tabs/bottle	30.00
0-5ºC	13076	TMB-HCl tablets	50 tabs/bottle	132.00

(TMA), Tetramethylene Glycol

(1,4-Butanediol dimethacrylate)
[H₂C=C₉CH₃)CO₂CH₂CH₂] ₂   F.W. 226.68
CAS #2082-81-7
Specific Gravity (H₂O=1) : 1.023
b.p. @4 mmHg 132-134ºC
Inhibited with 200 ppm hydroquinone monomethyl ether.

RT

21750

100ml

18.00

TMS, Tetramethylsilane, 99.9%

Si(CH₃)₄   M.W. 88.22
b.p. 26.6-26.7°C
Flashpoint: 28°C

An organo-silicon compound tetramethylsilane (TMS) is used as an internal standard for nuclear magnetic resonance (NMR). It is chemically inert and has a boiling point of 26.6°C which is lower than acetone (53.6°C), and other highly volatile liquids, such as diethyl ether (34.5°C) and 1,2-epoxy propane (36.4°C). It is soluble in most organic solvents but insoluble in water. All of these properties make TMS an ideal solvent for tissue drying, which preserves excellent tissue surface details. Dey, Sudip. Et al. (1989). A new rapid method of air-drying for scanning electron microscopy using tetramethylsilane. J. of Microscopy, Vol 156, pp. 259-261.

RT

21760

100ml

162.00

(TC-NBT), Thiocarbamyl Nitro-Blue Tetrazolium

(2,2'-Di[p-nitrophenyl]-5,5'-di[p-thiocarbamylphenyl] -3,3'-[3,3'-dimethoxy-4,4'-biphenylene]ditetrazolium chloride)
C₄₂H₃₂C_l2N₁₂O₆S₂   F.W. 935.8   CAS #36889-43-7

RT

21800

100mg

11.00

Nitro-Blue Tetrazolium

Please see Nitro-Blue Tetrazolium.

(TCH), Thiocarbohydrazide, Highest Purity

H₂NNHCSNHNH₂   F.W. 106.15   CAS #2231-57-4
b.p. 171°C (decomposed)
Osmiophilic reagent.

An indefinite shelf life due to a special purification process.

• Seligman A.M. et al (1965).Histochemical demonstration of some oxidized macromolecules with thiocarbohydrazide (TCH) or thiosemicarbazide (TSC) and osmium tetroxide. J. Histochem. Cytochem., 13:629
• Seligman, A.M. et al (1966). A new staining method (OTO) for enhancing contrast of lipid-containing membranes and droplets in osmium tetroxide fixed tissue with osmiophilic thiocarbohydrazide (TCH). J. Cell Biol., 30:424
• Thiery, J.P. (1967). Mise en evidence des polysaccharides sur coupes fines en microscopie electronique. J. Microsc., 6:987
• Lo, H.K. et al (1987). A modified periodic acid-thiocarbo-hydrazide-silver proteinate staining sequence for enhanced contrast and resolution of glycogen depositions by transmission electron microscopy. J. Histochem. Cytochem., 35:393
• Neiss, W.F. (1988). Enhancement of the periodic acid-Schiff (PAS) and periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) reaction in LR White sections. Histochemistry, 88:603
• Hanker, J.S. et al (1964). Osmiophilic reagents: new cytochemical principles for light and electron microscopy. Science, N.Y., 146:1039
• Derenzini, M., et al (1986). An improved periodic acid-thiosemicarbazide-osmium technique to reveal glyco-conjugates at the molecular level in situ. J. Histochem. Cytochem.,34:1161

RT

21900

5g

12.00

Thiosemicarbazide, Reagent, A.C.S.

NH₂CSNHNH₂   F.W. 91.14   CAS #79-19-6
m.p. 181-183°C
Assay >99.0%
References for use-see Thiocarbohydrazide

RT

22000

25g

12.00

Tissue Marking Dyes

Please see listing in General Supplies for Histology.

Toluene Reagent, A.C.S.

C₆H₅CH₃   FW 92.14   CAS #108-88-3
Specific Gravity (H₂O=1): 0.866
b.p. 110-1110C
Assay. 99.9%

Specifications:

Color (A.P.H.A.)	10
Residue after evaporation	0.001%
Subs. darkened by H2SO4	To pass test
Sulfur compound (as S)	0.003%
Water	0.03%

RT	22030		450ml	10.50
RT	22032		4x450ml	38.00

Toluidine Blue 0, Certified, C.I. #52040

(Basic blue; Methylene Blue T50 or T Extra)
C₁₅H₁₆N₃SCl   FW 305.83   CAS #92-31-9
Dye Content: approx. 85% min.
Solubility: 3.25% Water; 1.75% Alcohol; 3.5% Cellosolve; 5.5% Glycol; 0.0% Xylene
*-A general stain for epoxy thick sections. Barajas et al.(1981) Identification of renal neuroeffector junctions by electron microscopy of reembedded light microscopic autoradiograms of semithin sections. J. Ultrastruct. Res. 77: 379.Campbell, R.D., And Hermans, C.O. (1972). A rapid method for resectioning 0.5 4.0 micron epoxy sections for electron microscopy. Stain Technol . 47:115.

RT

22050

50g

29.00

Technical Tip

A Toluidine Blue/Basic Fuchsin Stain for GMA Embedded Tissue

Tricresyl Phosphate

(Tritolyl Phosphate)
(CH₃C₆H₄O)₃PO   F.W. 368.37   CAS #1330-78-5
Specific Gravity (H₂O=1): 1.16
A mixture of isomeric Tritolyl Phosphates, usually excluding the very toxic group ortho-isomer. Used to prepare benzoyl peroxide paste, which acts as a catalyst for water miscible resins.

RT

22150

225ml

22.00

2,4,6-Tri(Dimethylaminomethyl)-Phenol

Please see DMP-30.

RT	13600		50ml	2.50
RT	13600-SP		50 ml	4.00

2,4,6-Trinitrophenol

Please see Picric Acid .

RT

19550

100g

39.00

Tris (Hydroxymethyl) Aminomethane, Free-base, Reagent Grade

White crystalline powder
C₄H₁₁NO₃   F.W. 121.14   CAS #77-86-1
A 40% (w/v) aqueous solution. It is clear and colorless.
Assay >99.9%
Water .<0.2%
Heavy Metals.-2ppm

RT	11700		100g	15.00
RT	11720		500g	47.00

Tris (Hydroxymethyl) Aminomethane Maleate Buffer

Please see listing in Prepared Buffers

RT	11730		250ml	15.00
RT	11740		500ml	23.00

listing in Prepared Buffers

Triton® X-100

(Polyethylene glycol tert-octylphenyl ether)
CAS #9002-93-1
Specific Gravity (H₂O=1): 1.065

RT	22140		100ml	6.00
RT	22142		500ml	27.00

Triton® X-100, 1% Aqueous Solution

Prepared 1% Triton X-100 in Deionized-Distilled water. Used for whole-cell preparations. Marek, L.F., and Kelly, R.O. (1983). A simple technique for the visualization of whole mount cytoskeletons with transmission electron microscopy. Anat. Rec. 207, 365. Pudney, J., and Singer, R.H. (1980). Intracellular filament bundles in whole mounts of chick and human myoblasts extracted with Triton X-100. Tissue cell 12, 595.

RT

22145

500ml

8.00

Triton® X-100, 0.08% Aqueous Solution

A prepared solution for the cleaning of diamond knives.

RT

22146

500ml

7.00

Trypsin 1:100, Powder

CAS #9002-07-7
Extract from Porcine Pancreas.
Activity >100 NF units/mg
Used for whole cell preparations. (see references in Triton X-100)

RT

22200

25g

18.00

12-Tungstosilicic Acid

(Silicotungstic acid)
H₄[SiO₄.(W₃O₉)₄]·xH₂O   M.W. 2878.29+aq   CAS #11130-20-4

RT

22240

25g

34.00

Tyrode Solution

Ingredients: NaCl; KCl; CaCl2.6H2O; MgCl2.6H2O; NaHCO3; NaH2PO4; Glucose; and Distilled water.

Ordering Information:

RT	11760-05	Tyrode Solution	500ml	19.00
RT	11760-10	Tyrode Solution	1 L	27.00

Universal Immunoassay Buffer™, 10X

Immunoassay buffer is used as a 1X  in all immunoassays (immunofluorescence, IHC, ELISA, WB etc.) as a washing buffer  and it can be used as a dilution buffer for antibodies; however it does not contain any carrier proteins.

This buffer also does not contain any phosphate, sodium azide or mercury as preservatives and can be used for dilution of all antibodies, including peroxidase, antibodies to phosphoproteins. It is  not suitable for dilution of antibodies to S100 proteins.

Reagent Supplied: 10X Buffer

Application: Dilute this 10X buffer 10 times with DI water. (90 ml water + 10 ml this buffer – mixed well). Wash 3-5 times with this buffer in between the steps of your immunoassay protocol.

Storage: Room temperature

RT	25880-01	Universal Immunoassay Buffer, 10X	100 ml	40.00
RT	25880-10	Universal Immunoassay Buffer, 10X	1 L	85.00

Universal Antibody Dilution Buffer™

This ready-to-use Universal Antibody Dilution Buffer is designed for dilution of antibodies in all immunoassays, including immunofluorescence, IHC, ELISA, WB. This buffer does not contain any mammalian proteins, phosphate, sodium azide, or mercury preservatives and can be used for dilution of all antibodies, including peroxidase, and antibodies to phophoproteins. However, it is not suitable for dilution of antibodies to S100 proteins,

Reagent Supplied: Ready to use buffer

Application: Dilute antibodies, primary or secondary as recommended in the protocol by the manufacturer of these products.

Storage: 2–8°C. Diluted antibodies can be stored at 2–8°C for several weeks, depending upon the stability of antibodies.

2–8°C	25885-01	Universal Antibody Dilution Buffer	100 ml	40.00
2–8°C	25885-05	Universal Antibody Dilution Buffer	500 ml	99.00

Unicryl Embedding and Staining Kit

Please see listing in Embedding Media Kits.

RT

14660

kit

208.00

Uranyl Acetate, Reagent, A.C.S.

UO₂(OCOCH₃)₂·2H₂O   F.W. 424.14
CAS #541-09-3
Assay 98.0-102.0%
Specifications:

Insoluble Matters	0.01
Chloride	0.003%
Sulfate	0.01%
Alkalies (as SO4)	0.05%
Heavy Metals	0.002%
Iron	0.001%

A universal EM stain for thin sections, en-block staining, and negative staining.

RT

22400

25g

136.00

Uranyl Formate

UO₂(HCO₂)₂·H₂O

F.W. 378.08

CAS #16984-59-1

For ultra fine grain structure, our Uranyl Formate offers amazing advancement in negative staining for microscopy.

Specifications:

Purity	99.9-100%
Specific Gravity	3.695
Appearance	Yellow Crystals
Melting Point	110°C

Ordering Information:

RT	22450	Uranyl Formate	1 gram	$100.00
RT	22451	Uranyl Formate	5 grams	$475.00

Uranyl Magnesium Acetate

UO₂Mg(CH₃COO)₄

F.W.530.0

CAS # 20596-93-4

Used as a substitute for uranyl acetate in either double-staining techniques and also as a solo stain.

RT	22500	Uranyl Magnesium Acetate	5 grams	$175.00
RT	22501	Uranyl Magnesium Acetate	1 gram	$40.00

Uranyl Nitrate, Hexahydrate

Reagent Grade, A.C.S.
UO₂(NO₃)₂·6H₂O

F.W. 502.13

CAS #13520-83-7

Specifications:

Purity	99.9-100%
Density	2.807 g/cm3
Solubility	~66g/100g H2O
Melting Point	60°C
Boiling Point	118°C
Insoluble Matter	<0.005%
Chloride (Cl)	<0.002%
Sulfate (SO4)	<0.005%
Alkalies % Alkaline Earths (Sulfates)	<0.1%
Heavy Metals (as Pb)	<0.002%
Iron (Fe)	<0.002%
Substances Reducing Permanganate (as UIV)	<0.06%

A Universal EM Negative Stain used for viruses. In tissue samples it stabilizes nucleic acids and cell membranes. These solutions are more stable than uranyl acetate and they react primarily with negatively charged groups in the absence of phosphate ions.

RT

22600

Uranyl Nitrate

5 grams

$99.00

Uranyl Oxalate

(COO)₂ UO₂ ·.3H₂O

M.W.412.2

To adjust the pH of Uranyl compounds as well as a negative stain for electron microscopy.

RT

22510

Uranyl Oxalate

1 gram

$225.00

Uranyl Zinc Acetate

ZnUO₄(CH₃COO)₂

CAS # 33959-50-1

Used as a laboratory reagent in the determination of sodium concentrations of solutions.

RT

22610

Uranyl Zinc Acetate

5 grams

$175.00

Technical Tips:

Avoiding Contamination of Ultra-Thin Sections
Removal of Lead Citrate and Uranyl Acetate Precipitates on LR White Sections

Van Gieson’s Solution

To use with Weigert’s Iodine or Weigert’s Iron as a connective tissue
stain.

RT	26046-05	Van Gieson’s Solution	500ml	28.00	Add to Cart
RT	26046-06	Van Gieson’s Solution	1 L	49.00	Add to Cart

Vestopal® 310

Polyester Embedding Media

RT

22700

450ml

17.00

Vestopal Embedding Kit

Please see listing in Embedding Media Kits.

RT

14700

kit

35.00

Water Deionized, Reagent Grade A.C.S.

H₂O F.W. 18.02 CAS #7732-18-5
EMS Reagent Grade Water is typically prepared at 18 megohm/cm specific resistance using a reverse osmosis, mixed deionization, activated filtration and final filtration at 0.2 microns.
Color (APHA) <+/-5
Bacteriological purity 0 CFUs/L
Residue after evaporation 10ppm
Coliform negative

RT	22800-01	Deionized Water	1 gal	10.00
RT	22800-05	Deionized Water	5 gal	42.00

Weigert’s Iodine Solution

A histological stain. Demonstration of connective tissue is useful in cases of emphysema, arteriosclerosis and other vascular diseases

RT	26045-05	Weigert's Iodine Solution	500ml	25.00	Add to Cart
RT	26045-06	Weigert's Iodine Solution	1 L	37.00	Add to Cart

Weigert’s Iron Hematoxylin

Stain selectivity for nuclei, fungal, and general applications. Ready-to-use.

RT	26044-05	Weigert's Iron Hematoxylin A	500ml	33.00	Add to Cart
RT	26044-06	Weigert's Iron Hematoxylin A	1 L	55.00	Add to Cart
RT	26044-15	Weigert's Iron Hematoxylin B	500ml	25.00	Add to Cart
RT	26044-16	Weigert's Iron Hematoxylin B	1 L	43.00	Add to Cart

Wright's Stain, Certified

CAS #68988-92-1
Solubility: 0.091% Water; 0.23% Alcohol

RT

22720

25 g

31.00

Wright Staining Solution

For use as a blood stain. Ready-to-use.

RT	26060-05	Wright Stain Solution	500ml	30.00	Add to Cart
RT	26060-06	Wright Stain Solution	1 L	55.00	Add to Cart

Xylenes, Reagent, A.C.S.

(Xylol, Dimethylbenzene)
C₆H₄(CH₃)₂   FW 106.27   CAS #1330-20-7
Specific Gravity (H₂O=1): 0.865
b.p. 136-140°C
This reagent is a mixture of isomers (ortho, meta, and para) and may contain Ethylbenzene.

Specifications:

Color (A.P.H.A.)	10
Subs. darkened by H2SO4	To pass test
Sulfur Compounds (as S)	0.003%
Water	0.05%
Residue after evaporation	0.002%

RT

23400

450ml

8.00

For all of our Xylene Substitutes

Please see listing in Prepared Fixatives.

Z6040 Embedding Primer

This unique primer affords you a better adhesion between your resin and specimen that you are embedding and prevents the sample from pulling away from the resin during sectioning.

RT

50440-10

Z6040 Primer

100ml

25.00