Cryo
Supplies and Accessories
EMS UVC3 Ultraviolet Cryo Chamber
The EMS UVC3 Cryo Chamber has been specifically designed to provide an economical yet precisely controlled low temperature environment for polymerization of polymers and resins for cryo specimen preparation. It can also be used for dehydration and infiltration of cryo specimens. This simple and functional unit has a large insulated chamber and comprises fully automatic temperature control for the polymerization process of embedding resins and polymers.
Features:
- Liquid crystal display and PLC control
- Fan cooled electronic ballast for UV lamp tubes
- Temperature range can be set from -10°C to -40°C (14°F to -40°F)
- Includes memory function for Min/Max temperatures during any run
- 365nm ultraviolet lamps thermally shielded by dual pane Plexiglas®
- Holds up to 66 samples with BEEM® Cryo Capsule Holders
- Perfect for Unicryl, LR White, LR Gold, Lowicryls, JB-4, Quetols and all other resins cured by 365nm UV radiation
- Universal voltage
Operation
- Add dry ice to the chamber
- Set temperature (-10°C to -40°C / 14°F to -40°F)
- Turn on UV lamp and cure embedding resins
Specifications
| UV Light Source | Two 15-watt self-filtering long wavelength tubes 1600 µW/cm_ of 365nm UV radiation at 15cm (6") |
| Temperature Control | -10°C to -40°C (14°F to -40°F) Typically +/- 1.5 °C |
| Control Type | LCD Display with PLC with function buttons |
| Exterior Dimensions | 64.l L x 36.8 W x 48.9cm H (25.25" L x 14.50" W x 19.25" H) |
| Interior Dimensions | 56.5 L x 30.5 W x 33.7cm H (22.25" L x 12" W x 13.4" H) |
| Power Requirements | 110 - 240VAC, 50/60Hz (240V-0.5A, 110V-1A) |
| Weight | 11.4 kg (25 lbs.) / Shipping weight: 18.2kg (40 lbs.) |
| 70445-10 | EMS UVC3 Cryo Chamber, 120 VAC | each | 1,835.00 | Add to Cart |
| 70445-20 | EMS UVC3 Cryo Chamber, 220 VAC | each | 1,935.00 | Add to Cart |
| 70022-04 | EMS Embedding Capsule Holders | 4/pack | 36.00 | Add to Cart |
| 70120-04 | Gelatin "00" Capsule Holder | 4/pack | 36.00 | Add to Cart |
Specimen
Pins; Ultra Microtomy
Aluminum specimen mount pin for mounting samples for ultramicrotomy. Available in two types: Slotted Screw and Bullseye
| 70446 | Aluminum Specimen Pin, Bullseye | 50/ pack | 84.00 | Add to Cart |
| 70447 | Aluminum Specimen Pin, Slotted Screw | 50/ pack | 84.00 | Add to Cart |
Cryogenic-Gel
- Replaces the messy "runny" embedding media.
- Easy to use.
- Sticks to where you want it.
- Will not run – No waste.
It is a highly viscous, water-soluble embedding medium for frozen sections that "stays put". The gel is packaged in a pump-dispenser bottle that can instantly dispense the gel in any position. The dispenser has a built-in "guillotine" that cleanly severs the gel from the pump spouts and prevents any "stringy" medium from pulling away when the bottle is removed. To use, simply dispense gel on a room temperature block-holder. Use a cold heat extractor to form a base layer. Dispense additional gel. Place the tissue on top of the gel. Use a cold heat extractor to freeze tissue and form a block.
| 62806-01 | Cryo-Gel™, 4.3oz | each | 17.00 | Add to Cart |
| 62806-06 | Cryo-Gel™, 4.3oz | 12/cs | 176.00 | Add to Cart |
Tissue-Tek® O.C.T.
Compound
Sakura Finetek - This is a well known water soluble glycol’s and resins compound that provides an excellent specimen matrix for cryostat sectioning at temperatures of -10°C and below. It leaves no residue during the staining procedure.
| Cat. No. | Tissue-Tek | Description | Pack | Price | |
| 62550-01 | 4583 | O.C.T. Compound | 4 oz | 11.00 | Add to Cart |
| 62550-12 | 4583 | O.C.T. Compound | 12/cs | 112.00 | Add to Cart |
Tissue
Freezing Medium; TFM™
An embedding matrix for frozen sections. TFM™ is an ultra pure
formulation of water-soluble
glycols and resins that provides a solid bond between the tissue and the object
holder with the following features:
- Less Ice Artifacts: TFM's reduced water content minimizes freeze-fracturing.
- Less Curling: TFM allows you to pick up flat serial sections with ease.
- Freezes Faster: TFM freezes very fast and offers better turn-around time.
- Completely water soluble: TFM reduces tissue dislodging.
- Now available in 5 colors: clear, yellow green, red and blue
| 72592 | Tissue Freezing Medium, clear | 4 oz | 28.00 | Add to Cart |
| 72593 | Tissue Freezing Medium, clear | 12x4oz | 316.00 | Add to Cart |
| 72592-Y | Tissue Freezing Medium, Yellow | 4 oz | 29.00 | Add to Cart |
| 72593-Y | Tissue Freezing Medium, Yellow | 4x4oz | 114.00 | Add to Cart |
| 72592-G | Tissue Freezing Medium, Green | 4 oz | 29.00 | Add to Cart |
| 72593-G | Tissue Freezing Medium, Green | 4x4oz | 114.00 | Add to Cart |
| 72592-R | Tissue Freezing Medium, Red | 4 oz | 29.00 | Add to Cart |
| 72593-R | Tissue Freezing Medium, Red | 4x4oz | 114.00 | Add to Cart |
| 72592-B | Tissue Freezing Medium, Blue | 4 oz | 29.00 | Add to Cart |
| 72593-B | Tissue Freezing Medium, Blue | 4x4oz | 114.00 | Add to Cart |
LV CryoOil™
Oils are widely used in crystallography – as cryoprotectants when samples are flash cooled, and as barriers to the dehydration, solvent evaporation, and oxidation, that may occur in air near and above room temperature. The ice ring seen in the diffraction pattern of flash cooled protein crystals arise primarily from crystallization of the aqueous solution surrounding the crystal, not of the internal solvent. Oils can displace and replace this surrounding solution with little risk of damage due to osmotic shock.
Substantial dehydration-induced damage can easily occur between removed from a drop and flash cooling. Dehydration can be minimized by first transferring to an oil drop (using MicroSieve™, in MicroTool kit 1 and MicroTool Kit 2) before mounting the oil coated crystal on a MicroMount or Micromesh.
However, surrounding oil scatters X-rays, reducing diffraction signal-to-noise, and contributes thermal mass that reduces crystal cooling rates and increases the need for penetrating cryoprotectants. These problems are especially acute for the smallest crystals. When mineral oil, Paratone Oil and other high viscosity oils are used, the volume of surrounding oil can exceed the crystal volume by a factor of 10 or more., and can contribute excess drag that increases sample motion in a cryostream. For larger rod or plate shaped crystals, the surrounding oil exerts stresses when it freezes that can cause crystal cracking and mosaic broadening.
LV CryoOil™ Is the best solution to your oil needs. It has the lowest viscosity of any available perfluoropolyether oil (1/10 that of Paratone oil and compatible to that of vegetable oil) and very low surface tension (less than 1/3 that of water). Consequently, a dip in this oil followed by gentle tapping to shake off excess can yield protective oil films on your crystals of as little as 10 µm thick.
LV CryoOil™ has extremely low vapor pressure, excellent chemical inert and excellent thermal stability.
Ordering:
LV CryoOil™ comes in each vial 1.5 ml, enough to protect hundreds of crystals.
| 60166 | LV CryoOil™ | 1.5 ml vial | 58.00 | Add to Cart |
Quick
Freeze; Shur/Freeze, Environmentally Safe
A high purity 1, 1, 1, 2 - Tetrafluoroethane (R134a) that you can rely on to solidify paraffin sections of fresh tissue without disrupting cellular structure. A short spray burst will instantly freeze small area to -45ºC/-5ºF. Environmentally Safe and non-flammable.
| 72595 | Quick Freeze; Shur/Freeze | 10 oz | 34.00 | Add to Cart |
| 72596 | Quick Freeze; Shur/Freeze | 6x10 oz | 376.00 | Add to Cart |
CytoCool II
For the Flash freezing of tissue specimens in the Cryostat as well as being used to harden difficult to cut tissue on a microtome during the sectioning. CytoCool II is an aerosol that does not contain any ozone depleting (CFC'S). Controlling the tissue freezing is simply done by varying the finger pressure on the trigger nozzle. This unique feature helps to prevent over cooling of the specimen.
| 72597 | CytoCool II Aerosol | 11 oz | 29.00 | Add to Cart |
| 72597-12 | CytoCool II Aerosol | 12x11 oz | 312.00 | Add to Cart |
Cryo-Gloves®
We now offer cryogenic personal protective garments of the highest quality. Cryo-Gloves® and Cryo-Aprons® are essential accessories for those working in ultra cold environments. Providing effective protection in a wide variety of applications .
Features
- Protection from –135°C to 150°C
- Superior thermal protection
- Water-resistant
- Comfortable / Lightweight / Excellent dexterity
- Breathable / Stay-dry lining.
Applications
- Ultra low and Blast freezers
- Cold rooms
- Dry ice handling
- Liquid Nitrogen, liquid oxygen, liquid helium
Environment
- Clinical Laboratory
- Bio-Medical
- Blood Banks
- Frozen food processing
- Pharmaceutical
| 71070 | Wrist Cryo-Gloves®, Small | 12" long | 1 pair | 130.00 | Add to Cart |
| 71071 | Wrist Cryo-Gloves®, Medium | 12" long | 1 pair | 130.00 | Add to Cart |
| 71072 | Wrist Cryo-Gloves®, Large | 12" long | 1 pair | 130.00 | Add to Cart |
| 71072-X | Wrist Cryo-Gloves®, X-Large | 12" long | 1 pair | 130.00 | Add to Cart |
| 71073 | Mid Arm Cryo-Gloves®, Small | 14" to 15" long | 1 pair | 138.00 | Add to Cart |
| 71074 | Mid Arm Cryo-Gloves®, Medium | 14" to 15" long | 1 pair | 138.00 | Add to Cart |
| 71075 | Mid Arm Cryo-Gloves®, Large | 14" to 15" long | 1 pair | 138.00 | Add to Cart |
| 71075-X | Mid Arm Cryo-Gloves®, X-Large | 14" to 15" long | 1 pair | 138.00 | Add to Cart |
| 71076 | Elbow Cryo-Gloves®, Small | 18" to 20" long | 1 pair | 164.00 | Add to Cart |
| 71076-M | Elbow Cryo-Gloves®, Medium | 18" to 20" long | 1 pair | 164.00 | Add to Cart |
| 71077 | Elbow Cryo-Gloves®, Large | 18" to 20" long | 1 pair | 164.00 | Add to Cart |
| 71078 | Elbow Cryo-Gloves®, X-Large | 18" to 20" long | 1 pair | 164.00 | Add to Cart |
| 71079-S | Shoulder Cryo-Gloves®, Small | 27" to 28" long | 1 pair | 208.00 | Add to Cart |
| 71079-M | Shoulder Cryo-Gloves®, Medium | 27" to 28" long | 1 pair | 208.00 | Add to Cart |
| 71079-L | Shoulder Cryo-Gloves®, Large | 27" to 28" long | 1 pair | 208.00 | Add to Cart |
| 71079-X | Shoulder Cryo-Gloves®, X-Large | 27" to 28" long | 1 pair | 208.00 | Add to Cart |
Cryo-Gloves® and Cryo-Aprons® are registered trademarks of Tempshield, Mt Desert ME.
Cryo-Apron®
These aprons are 100% water-proof with adjustable straps, for neck and waist.
| 71080 | Cryo-Apron®, Small 36" | each | 128.00 | Add to Cart |
| 71081 | Cryo-Apron®, Medium 42" | each | 140.00 | Add to Cart |
| 71082 | Cryo-Apron®, Large 48" | each | 152.00 | Add to Cart |
| 71082-X | Cryo-Apron®, X-Large 54" | each | 160.00 | Add to Cart |
Safety Goggles
| 64475-01 | Safety Goggle, 4 Plastic Vent | each | 3.75 | Add to Cart |
| 64475-10 | Safety Goggle, 4 plastic Vent | 10/pk | 34.00 | Add to Cart |
| 64476-01 | Safety Goggle, Perforated Frame | each | 3.75 | Add to Cart |
| 64476-10 | Safety Goggle, Perforated Frame | 10/pk | 34.00 | Add to Cart |
| 64477-01 | Safety Goggle, Perforated Frame, Small Size (5 ¾” x 2 ½”) | each | 3.75 | Add to Cart |
| 64477-10 | Safety Goggle, Perforated Frame, Small Size (5 ¾” x 2 ½”) | 10/pk | 34.00 | Add to Cart |
Articles of Interest
Cryo Sectioning and the Use of Diamond Knives
