Cryo
Supplies and Accessories

Specimen
Pins; Ultra Microtomy
Aluminum specimen mount pin for mounting samples for ultramicrotomy. Available in two types: Slotted Screw and Bullseye
| 70446 | Aluminum Specimen Pin, Bullseye | 50/ pack | 75.00 | |
| 70447 | Aluminum Specimen Pin, Slotted Screw | 50/ pack | 75.00 |

Cryogenic Grid Storage Box
This specimen grid box is a tool for storing or transferring cryogenic TEM specimen grids.
- Four diamond shaped slots
- Non-static cover is held in place with stainless steel screw, which is tapped in the center of the box.
- Box fits the FEI Vitrobot™, Gatan 626™, Gatan 3500™.
- Available with lid or without lid.
| 71166-10 | Cryo Grid Box, Round, w/Lid | each | 9.25 | Add to Cart |
| 71166-20 | Cryo Grid Box, Square, w/Lid | each | 9.75 | Add to Cart |
| 71166-30 | Cryo Grid Box, Round, wo/Lid | each | 6.90 | Add to Cart |
Cryogenic Grid Box Handling Tool
This tool has one end which is threaded and fits into the center hole of the Cryogenic Grid Storage Box (where the screw goes in to secure the lid) for moving the box in and out of the cryogenic chamber.
| 71166-50 | Cryogenic Grid Box Handling Tool | each | 19.50 | Add to Cart |


Cryogenic-Gel
- Replaces the messy "runny" embedding media.
- Easy to use.
- Sticks to where you want it.
- Will not run – No waste.
It is a highly viscous, water-soluble embedding medium for frozen sections that "stays put". The gel is packaged in a pump-dispenser bottle that can instantly dispense the gel in any position. The dispenser has a built-in "guillotine" that cleanly severs the gel from the pump spouts and prevents any "stringy" medium from pulling away when the bottle is removed. To use, simply dispense gel on a room temperature block-holder. Use a cold heat extractor to form a base layer. Dispense additional gel. Place the tissue on top of the gel. Use a cold heat extractor to freeze tissue and form a block.
| 62806-01 | Cryo-Gel™, 4.3oz | each | 12.00 | |
| 62806-06 | Cryo-Gel™, 4.3oz | 12/cs | 126.00 |



Tissue-Tek® O.C.T.
Compound
Sakura Finetek - This is a well known water soluble glycols and resins compound that provides an excellent specimen matrix for cryostat sectioning at temperatures of -10°C and below. It leaves no residue during the staining procedure.
| Cat. No. | Tissue-Tek | Description | Pack | Price | |
| 62550-01 | 4583 | O.C.T. Compound | 4 oz | 10.50 | |
| 62550-12 | 4583 | O.C.T. Compound | 12/cs | 104.00 |


Tissue
Freezing Medium; TFM™
An embedding matrix for frozen sections. TFM™ is an ultra pure
formulation of water-soluble
glycols and resins that provides a solid bond between the tissue and the object
holder with the following features:
- Less Ice Artifacts: TFM's reduced water content minimizes freeze-fracturing.
- Less Curling: TFM allows you to pick up flat serial sections with ease.
- Freezes Faster: TFM freezes very fast and offers better turn-around time.
- Completely water soluble: TFM reduces tissue dislodging.
- Now available in 5 colors: clear, yellow green, red and blue
LV CryoOil™
Oils are widely used in crystallography – as cryoprotectants when samples are flash cooled, and as barriers to the dehydration, solvent evaporation, and oxidation, that may occur in air near and above room temperature. The ice ring seen in the diffraction pattern of flash cooled protein crystals arise primarily from crystallization of the aqueous solution surrounding the crystal, not of the internal solvent. Oils can displace and replace this surrounding solution with little risk of damage due to osmotic shock.
Substantial dehydration-induced damage can easily occur between removed from a drop and flash cooling. Dehydration can be minimized by first transferring to an oil drop (using MicroSieve™, in MicroTool kit 1 and MicroTool Kit 2) before mounting the oil coated crystal on a MicroMount or Micromesh.
However, surrounding oil scatters X-rays, reducing diffraction signal-to-noise, and contributes thermal mass that reduces crystal cooling rates and increases the need for penetrating cryoprotectants. These problems are especially acute for the smallest crystals. When mineral oil, Paratone Oil and other high viscosity oils are used, the volume of surrounding oil can exceed the crystal volume by a factor of 10 or more., and can contribute excess drag that increases sample motion in a cryostream. For larger rod or plate shaped crystals, the surrounding oil exerts stresses when it freezes that can cause crystal cracking and mosaic broadening.
LV CryoOil™ Is the best solution to your oil needs.
It has the lowest viscosity of any available perfluoropolyether oil (1/10 that of Paratone oil and compatible to that of vegetable oil) and very low surface tension (less than 1/3 that of water). Consequently, a dip in this oil followed by gentle tapping to shake off excess can yield protective oil films on your crystals of as little as 10 µm thick.
LV CryoOil™ has extremely low vapor pressure, excellent chemical inert and excellent thermal stability.
Ordering:
LV CryoOil™ comes in each vial 1.5 ml, enough to protect hundreds of crystals.
| 60166 | LV CryoOil™ | 1.5 ml vial | 50.00 |

Quick
Freeze; Shur/Freeze, Environmentally Safe
A high purity 1, 1, 1, 2 - Tetrafluoroethane (R134a) that you can rely on to solidify paraffin sections of fresh tissue without disrupting cellular structure. A short spray burst will instantly freeze small area to -45ºC/-5ºF. Environmentally Safe and non-flammable.
| 72595 | Quick Freeze; Shur/Freeze | 10 oz | 31.00 | |
| 72596 | Quick Freeze; Shur/Freeze | 6x10 oz | 340.00 |

Cryo-Gloves®
We now offer cryogenic personal protective garments of the highest quality. Cryo-Gloves® and Cryo-Aprons® are essential accessories for those working in ultra cold environments. Providing effective protection in a wide variety of applications .
Features
- Protection from –135°C to 150°C
- Superior thermal protection
- Water-resistant
- Comfortable / Lightweight / Excellent dexterity
- Breathable / Stay-dry lining.
Applications
- Ultra low and Blast freezers
- Cold rooms
- Dry ice handling
- Liquid Nitrogen, liquid oxygen, liquid helium
Environment
- Clinical Laboratory
- Bio-Medical
- Blood Banks
- Frozen food processing
- Pharmaceutical
Cryo-Gloves® and Cryo-Aprons® are registered trademarks of Tempshield, Mt Desert ME.

Cryo-Apron®
These aprons are 100% water-proof with adjustable straps, for neck and waist.
| 71080 | Cryo-Apron®, Small 36" | each | 120.00 | |
| 71081 | Cryo-Apron®, Medium 42" | each | 130.00 | |
| 71082 | Cryo-Apron®, Large 48" | each | 142.00 | |
| 71082-X | Cryo-Apron®, X-Large 54" | each | 150.00 |
CryoVial®
Specially Formulated Polypropylene, is certified Rnase-free, Dnase-free, Pyrogen-free, and DNA-free.
Designed for storage of biological material, human or animal
cells, at temperatures as low as -190ºC.- The cap features a long skirt for one hand aseptic technique.
- A super fast thread design that allows tightening or removal with a mere 11⁄4" turn, and an inside thread design that will not contribute to possible contamination.
- Tubes are provided with white marking area for sample identification, and can be color-coded by the use of a CAPINSERT®.
- These CRYOVIALS® are compatible with most storage systems.
- Vials are sterilized by gamma irradiation, and are packaged in unique tempered-proof, reseal-able, safety-lock-bags of 100, and 10bags to a case.
- The closures and tubes are both manufactured of polypropylene having the same coefficient of expansion, which further enhances the leak-proof qualities of these vials at changing temperatures.
Tubes are available in three series:
Series T309
- LIP-SEAL DESIGN TUBE
- TUBE WITH EXTERNALTHREADS
Series T310
- SILICONE WASHER SEAL
- TUBE WITH EXTERNAL THREADS
Series T311
- SILICONE WASHER SEAL
- TUBE WITH INTERNAL THREADS
Ordering: All Cryovials® are sold by case quantity (10x100 per case)
** Item 61801-10A (10ml Self-Standing 17x84mm) are only 50x10 per case.

CAPINSERT™ for Cryovial® Tubes
Made from polypropylene. Color-coded inserts fit precisely into the cap of the CRYOVIAL® above for color identification. CAPINSERTS® are sold 500 per bag.

Wide Mouth Cryogenic Vial
A wide mouth vial with 15 ml capacity. Useful for the storage and retrieval of solid specimens, such as bone, tumor, and organ samples. Made from polypropylene with high- density polyethylene screw closures. Selfstanding. Radiation sterilized, noncytotoxic, non-pyrogenic. Caps are attached in 15 bags of 5 vials each or 75 per case.
| 61850-15 | Wide Mouth Cryogenic Vial | 75/case | 104.00 |

Cryo-Lok
Cryogenic Vials
CYO-LOK vials are ideal for storage or transport of material of extremely low temperatures. The vials have external threads, providing a smooth and uniform inner surface. This eliminates the problem of air born contamination and/or sample loss so common when vials with internal threads are used. The screw top incorporates a molded-in sealing ring, which assures a tight leak-proof seal.
- Graduated in 0.5ml increments
- Conical bottom with slotted skirt
- For use in vapor phase of liquid nitrogen
- Color Caps also Available*

Cryogenic
Vial Rack
Made from durable polycarbonate. Its unique design with slotted cavities accepts vials with slotted skirts. When placed in the holes the vials become locked in position, enabling the user to attach or remove the cap with one hand. It accommodates up to 50 vials(½ " or 13.5mm diameter) in a 4x10 array with alphanumeric indexing. Autoclavable at 121°C.
Measures: 7½"x4½"x 7/8" (190x108x22mm)
| 62015-01 | Cryogenic Vial Rack | each | 13.00 | |
| 62015-04 | Cryogenic Vial Rack | 4/pack | 50.00 |

Cryostore® Boxes
Made from strong Polycarbonate, these durable cryogenic storage boxes are designed to be used at temp-eratures between -190ºC and +121ºC and are auto-clavable. Different models are available to accommodate either 81 or 100 Cryovial® tubes from 1ml to 5ml.
A transparent cover allows the user to see the contents of the box, and is keyed to the base to prevent misalignment. Being printed with a series of squares (numbered from 1 to 81, or from 1 to 100), the surface accepts writing with markers for better inventory control.
A unique color-coding system uses color plastic grids inserted to separate the cover from the base of the 81-place box. Boxes are made to accept 100 tubes have a colored base instead of grid. A choice of four popular colors is available: blue, green, red and yellow.
Removal of vials is facilitated by an innovative tool called the Vial Picker, which is supplied with each storage box.
- Writing surface has numbered squares for easy sample identification
- Stackable
- Vials readily visible through transparent cover
- Four colors available for better color-coding
- Cover and base are keyed to prevent misalignment
- Drain holes under base
- Made to fit freezer metal racks
- Writing surface for identifying base and/or cover
- Autoclavable at 120°C, 15 psig (1 bar) for 20 minutes
- Numeric identification of each vial
- Air vents minimizing condensation
| Catalog # | Series # | For Tubes |
Color | Qty/Pk | Qty/cs | Price |
Series T314- 281: Size: 133 x 133 x 52 H (51⁄4" x 51⁄4" x 21⁄16"H) 81 Positions
| 61810-B | 281B | 1 to 2ml | Blue | 4 | 24 | 240.00 | |
| 61810-G | 281G | 1 to 2ml | Green | 4 | 24 | 240.00 | |
| 61810-R | 281R | 1 to 2ml | Red | 4 | 24 | 240.00 | |
| 61810-Y | 281Y | 1 to 2ml | Yellow | 4 | 24 | 240.00 |
Series T314-481: Size: 133 x 133 x 76 H (51⁄4" x 51⁄4" x 3"H) 81 Positions
| 61811-B | 481B | 3 to 4ml | Blue | 6 | 12 | 150.00 | |
| 61811-G | 481G | 3 to 4ml | Green | 6 | 12 | 150.00 | |
| 61811-R | 481R | 3 to 4ml | Red | 6 | 12 | 150.00 | |
| 61811-Y | 481Y | 3 to 4ml | Yellow | 6 | 12 | 150.00 |
Series T314-581: Size: 133 x 133 x 95 H (51⁄4" x 51⁄4" x 33⁄4"H) 81 Positions
| 61812-B | 581B | 3 to 5ml | Blue | 5 | 10 | 128.00 | |
| 61812-G | 581G | 3 to 5ml | Green | 5 | 10 | 128.00 | |
| 61812-R | 581R | 3 to 5ml | Red | 5 | 10 | 128.00 | |
| 61812-Y | 581Y | 3 to 5ml | Yellow | 5 | 10 | 128.00 |
Series T314-2100: Size: 133 x 133 x 52 H (51⁄4" x 51⁄4" x 21⁄16"H) 100 Positions
| 61813-B | 2100B | 1 to 2ml | Blue | 4 | 24 | 244.00 | |
| 61813-G | 2100G | 1 to 2ml | Green | 4 | 24 | 244.00 | |
| 61813-R | 2100R | 1 to 2ml | Red | 4 | 24 | 244.00 | |
| 61813-Y | 2100Y | 1 to 2ml | Yellow | 4 | 24 | 244.00 |

Cryo
Storage Boxes
Made from heavy-duty chipboard. These boxes store vials neatly and fit most standard rack systems. They come with dividers and are slotted for cross-ventilation. The small box is 5½ " (132mm) square, 2" (50mm) high and contains divided spaces for up to 81 2ml vials. The medium box is 5½ square", 3" high and contains divided spaces for up to 81 2.5 and 3.5 ml vials. The jumbo is 6" (132mm) square, 3" (50mm) high and contains divided spaces for up to 100 vials.
| 62010-10 | Small Cryo Storage Box | 4/pack | 22.00 | |
| 62010-20 | Medium Cryo Storage Box | 4/pack | 25.00 | |
| 62010-30 | Jumbo Cryo Storage Box | 4/pack | 23.00 |

Liquid
Nitrogen-Cooled Mortar
A stainless steel mortar is suspended by three brackets above a stainless steel bowl which holds the liquid nitrogen. The bowl is nested in a well insulated polyethylene housing. The 4"(102mm) insulated handle is removable for saving space. The housing measures 6½"(165mm) in diameter, 4½" high, and has three suction cup-feet. Now frozen tissue samples can be ground without the hazard of liquid nitrogen droplets flying into personnel
| 64470 | Liquid Nitrogen-Cooled Mortar | set | 265.00 | |
| 64471 | Spare mortar bowl | each | 89.00 | |
| 64472 | Ceramic Paste only, 180mm long | each | 30.00 |

Cryogenic
Workstation
Corning® Cryogenic Workstation features a reusable cryogenic vial rack with a removable storage rack. Rack holds to 30 vials. The tray can be used to hold water or crushed ice. The racks and trays are made of autoclavable polycarbonate.
Overall measurement. 14½"(L)x6"(W)x3"(H) (216mmx184mmx83mm)
| 62040 | Cryogenic Workstation | each | 112.00 | |
| 62041 | Reusable Vial Rack Only | each | 92.00 | |
| 62042 | Reusable Storage Tray Only | each | 30.00 |

Cryo Transport Box
Made from high impact polystyrene
- Ideal for transporting Cryo Vials
- Durable construction
- Organizes small amounts of Cryo Vials
- Holds 1.0 – 2.0ml Cryo Vials
| 62004-05 | Cryo Transport Box for 5 Tubes | 20/pk | 50.00 | |
| 62004-10 | Cryo Transport Box for 10 Tubes | 10/pk | 32.00 |

Nalgene® Polyethylene
Dewar Flask
High density polyethylene Dewar Flask with vented and insulated HDPE covers. They are unbreakable, chemically resistant, and withstand temperatures from (-196) - (100º C) (-321 to +212º F). Double walls are filled with foamed urethane to minimize heat exchange. May be used for short term storage of dry ice-solvent, liquid nitrogen, or for use as a hot bath. They are ideal for storing frozen biological specimens. All available sizes feature molded-in-ribs for stability and a carrying handle on the 1, 2, and 4 liter sizes.
| Catalog | Capacity L | Overall height mm/in | Mouth I.D. mm/in | Price | |
| 62038-01 | 1 | 299/9 | 95/3 | 196.00 | |
| 62038-02 | 2 | 260 | 121 | 192.00 | |
| 62038-04 | 4 | 324 | 146 | 198.00 | |
| 62038-10 | 10 | 457 | 197 | 338.00 |
Narrow
Mouth Dewars
Lab grade dewars. Made from borosilicate glass and vacuumed to 10-7 torr, without pads between walls, eliminating heat transfer for maximum efficiency. They come with low evaporation stopper and weighted base.
Spherical Dewar Flask
Metal Casting does not extend the full length of the flask. Florence flask design, offers the most efficient way of holding liquid nitrogen. Available with an optional Fiber Glass Caddie for safety, easy carrying, and decanting liquids from the above flasks.

Cryogenics Liquid Nitrogen Transfer Vessels
Thermo-Flask provides safe extended temperature retention for specimens stored in liquid nitrogen or solid CO2, and thus have a broad range of applications in industrial, educational, and clinical fields.
Thermo-Flask features borosilicate glass inner liners which have been evacuate to 1x10-5mm of Hg to provide maximum coolant retention.
Example of Possible Retention Times at 80% Full Liquid Nitrogen Retention:
1.
Stainless Steel Thermo-Flask® with Handle and Lid
- Durable, corrosion-resistant stainless steel exterior construction with carrying handle and vented lid secured by toggle clamps.
- Recommended for the safe transport of liquid nitrogen.
| Catalog | Capacity liters | I.D. inches |
O.D. inches | Height inches |
Set Price | |
| 61891-01 | 1.0 | 3 1⁄3 | 4 5⁄8 | 9 |
289.00 | |
| 61891-02 | 2.0 | 4 1⁄4 | 5 5⁄8 | 10 1/2 |
335.00 | |
| 61891-04 | 4.5 | 6 | 7 1⁄4 | 13 3/4 |
481.00 |

2. Shallow Wide Mouth Flasks
- Convenient dish-shaped design is ideal for bench top use with crushed ice, dry- ice, or alcohol.
- Also great as a hot liquid container.
- Stainless steel jacket and cushioned base (lids not included) will provide years of trouble-free use.
- Excellent for use as a cold trap.
3. Plastic Wide Mouth Flask
Please see 61891.

4. Enameled Steel Thermo-Flasks
- Safe storage of liquified gases, temperature retention, and versatility at an economical price.
- Enameled steel exteriors, borosilicate glass liners and cushioned bases.
- Model 2121 (62029) includes an insulated lid and fold-down carrying handle.
- Ideal for cold traps.
| Catalog | O.D. inches | I.D. inches | Height inches | Capacity liters | Set Price | |
| 62022 | 3¾ | 2 3/8 | 11¼ | 0.65 | 282.00 | |
| 62023 | 4¼ | 3 | 11.0 | 1.1 | Replaced by 61891-01 | |
| 62029 | 9 1/8 | 8 | 17 1/8 | 10.0 | 1085.00 |
Ultra-Violet Lamps
Please see our complete listing.

CryoStore™ Canes
Made from aluminum for liquid nitrogen use. It stores up to 6 standard cryo vials. CryoStore™ Tabs are a color coded system which adheres to the end of the cane for quick and easy sample retrieval. Available in five colors.

CryoFlex® Tubing
CryoFlex® is a hollow tube from polyethylene which is useful for encasing and sealing cryo vials, when storing samples immersed in liquid nitrogen. The tubing measures 9⁄16" diameter x 1911⁄16" long (1.4 x 50cm)
| 61083-10 | CryoFlex® Tubing 15/pk | 15/pack | 76.00 |
CryoSleeve®
Clear polyvinyl chloride. Ideal for enclosing Cryo Storage Canes (EMS #61080-10) for extra security during handling and storage. Fits over most other canes. Will not become brittle during freezing or thawing. Will not shred or tear after repeated use like cardboard. Length: 1013⁄16" (27.5cm L).
| 61082-10 | Cryo Sleeve® | 100/pack | 68.00 |

Tissue-Tek® Cryomold®;
Sakura Finetek
These disposable plastic Cryomolds produce a uniformly shaped flat surface specimen block with tissue freezing medium (O.C.T.) for frozen sectioning, Standard Cryomold fits directly into Cryobar® freezing wells of the Tissue-Tek® II microtome cryostat. Use a Cryomold® adapter to adapt smaller biopsy and intermediate Cryomold® molds to freezing wells on the Cryobar® unit.

Tissue-Tek® Cryomold®;
Sakura Finetek
These disposable plastic Cryomolds produce a uniformly shaped flat surface specimen block with tissue freezing medium (O.C.T.) for frozen sectioning, Standard Cryomold fits directly into Cryobar® freezing wells of the Tissue-Tek® II microtome/cryostat. Use a Cryomold® adapter to adapt smaller biopsy and intermediate Cryomold® molds to freezing wells on the Cryobar® unit.
| Cat. No. | Tissue-Tek | Description | Pack | Price | |
| 62534-25 | 4557 | Standard, 25x20x5mm | 100/pk | 19.50 | |
| 62534-10 | 4565 | Biopsy, 10x10x5mm | 100/pk | 19.50 | |
| 62534-15 | 4566 | Intermediate 15x15x5mm | 100/pk | 19.50 |
Articles of Interest
Cryo Sectioning and the Use of Diamond Knives

