Cryo
Supplies and Accessories
Specimen
Pins; Ultra Microtomy
Aluminum specimen mount pin for mounting samples for ultramicrotomy. Available in two types: Slotted Screw and Bullseye
| 70446 | Aluminum Specimen Pin, Bullseye | 50/ pack | 75.00 |  |
| 70447 | Aluminum Specimen Pin, Slotted Screw | 50/ pack | 75.00 | |
Cryogenic Grid Storage Box
This specimen grid box is a tool for storing or transferring cryogenic TEM specimen grids.
- Four diamond shaped slots
- Non-static cover is held in place with stainless steel screw, which is tapped in the center of the box.
- Box fits the FEI Vitrobot™, Gatan 626™, Gatan 3500™.
- Available with lid or without lid.
| 71166-10 | Cryo Grid Box, Round, w/Lid | each | 9.25 | Add to Cart |
| 71166-20 | Cryo Grid Box, Square, w/Lid | each | 9.75 | Add to Cart |
| 71166-30 | Cryo Grid Box, Round, wo/Lid | each | 6.90 | Add to Cart |
Cryogenic Grid Box Handling Tool
This tool has one end which is threaded and fits into the center hole of the Cryogenic Grid Storage Box (where the screw goes in to secure the lid) for moving the box in and out of the cryogenic chamber.
| 71166-50 | Cryogenic Grid Box Handling Tool | each | 19.50 | Add to Cart |
Cryogenic-Gel
- Replaces the messy "runny" embedding media.
- Easy to use.
- Sticks to where you want it.
- Will not run – No waste.
It is a highly viscous, water-soluble embedding medium for frozen sections that "stays put". The gel is packaged in a pump-dispenser bottle that can instantly dispense the gel in any position. The dispenser has a built-in "guillotine" that cleanly severs the gel from the pump spouts and prevents any "stringy" medium from pulling away when the bottle is removed. To use, simply dispense gel on a room temperature block-holder. Use a cold heat extractor to form a base layer. Dispense additional gel. Place the tissue on top of the gel. Use a cold heat extractor to freeze tissue and form a block.
| 62806-01 | Cryo-Gel™, 4.3oz | each | 12.00 | |
| 62806-06 | Cryo-Gel™, 4.3oz | 12/cs | 126.00 | |
Tissue-Tek® O.C.T.
Compound
Sakura Finetek - This is a well known water soluble glycol’s and resins compound that provides an excellent specimen matrix for cryostat sectioning at temperatures of -10°C and below. It leaves no residue during the staining procedure.
| Cat. No. | Tissue-Tek | Description | Pack | Price | |
| 62550-01 | 4583 | O.C.T. Compound | 4 oz | 10.50 | |
| 62550-12 | 4583 | O.C.T. Compound | 12/cs | 104.00 | |
Tissue
Freezing Medium; TFM™
An embedding matrix for frozen sections. TFM™ is an ultra pure
formulation of water-soluble
glycols and resins that provides a solid bond between the tissue and the object
holder with the following features:
- Less Ice Artifacts: TFM's reduced water content minimizes freeze-fracturing.
- Less Curling: TFM allows you to pick up flat serial sections with ease.
- Freezes Faster: TFM freezes very fast and offers better turn-around time.
- Completely water soluble: TFM reduces tissue dislodging.
- Now available in 5 colors: clear, yellow green, red and blue
| 72592 | Tissue Freezing Medium, clear | 4 oz | 23.50 | |
| 72593 | Tissue Freezing Medium, clear | 12x4oz | 276.00 | |
| 72592-Y | Tissue Freezing Medium, Yellow | 4 oz | 25.00 | |
| 72593-Y | Tissue Freezing Medium, Yellow | 4x4oz | 95.00 | |
| 72592-G | Tissue Freezing Medium, Green | 4 oz | 25.00 | |
| 72593-G | Tissue Freezing Medium, Green | 4x4oz | 95.00 | |
| 72592-R | Tissue Freezing Medium, Red | 4 oz | 25.00 | |
| 72593-R | Tissue Freezing Medium, Red | 4x4oz | 95.00 | |
| 72592-B | Tissue Freezing Medium, Blue | 4 oz | 25.00 | |
| 72593-B | Tissue Freezing Medium, Blue | 4x4oz | 95.00 | |
LV CryoOil™
Oils are widely used in crystallography – as cryoprotectants when samples are flash cooled, and as barriers to the dehydration, solvent evaporation, and oxidation, that may occur in air near and above room temperature. The ice ring seen in the diffraction pattern of flash cooled protein crystals arise primarily from crystallization of the aqueous solution surrounding the crystal, not of the internal solvent. Oils can displace and replace this surrounding solution with little risk of damage due to osmotic shock.
Substantial dehydration-induced damage can easily occur between removed from a drop and flash cooling. Dehydration can be minimized by first transferring to an oil drop (using MicroSieve™, in MicroTool kit 1 and MicroTool Kit 2) before mounting the oil coated crystal on a MicroMount or Micromesh.
However, surrounding oil scatters X-rays, reducing diffraction signal-to-noise, and contributes thermal mass that reduces crystal cooling rates and increases the need for penetrating cryoprotectants. These problems are especially acute for the smallest crystals. When mineral oil, Paratone Oil and other high viscosity oils are used, the volume of surrounding oil can exceed the crystal volume by a factor of 10 or more., and can contribute excess drag that increases sample motion in a cryostream. For larger rod or plate shaped crystals, the surrounding oil exerts stresses when it freezes that can cause crystal cracking and mosaic broadening.
LV CryoOil™ Is the best solution to your oil needs.
It has the lowest viscosity of any available perfluoropolyether oil (1/10 that of Paratone oil and compatible to that of vegetable oil) and very low surface tension (less than 1/3 that of water). Consequently, a dip in this oil followed by gentle tapping to shake off excess can yield protective oil films on your crystals of as little as 10 µm thick.
LV CryoOil™ has extremely low vapor pressure, excellent chemical inert and excellent thermal stability.
Ordering:
LV CryoOil™ comes in each vial 1.5 ml, enough to protect hundreds of crystals.
| 60166 | LV CryoOil™ | 1.5 ml vial | 50.00 | |
Quick
Freeze; Shur/Freeze, Environmentally Safe
A high purity 1, 1, 1, 2 - Tetrafluoroethane (R134a) that you can rely on to solidify paraffin sections of fresh tissue without disrupting cellular structure. A short spray burst will instantly freeze small area to -45ºC/-5ºF. Environmentally Safe and non-flammable.
| 72595 | Quick Freeze; Shur/Freeze | 10 oz | 31.00 | |
| 72596 | Quick Freeze; Shur/Freeze | 6x10 oz | 340.00 | |
Cryo-Gloves®
We now offer cryogenic personal protective garments of the highest quality. Cryo-Gloves® and Cryo-Aprons® are essential accessories for those working in ultra cold environments. Providing effective protection in a wide variety of applications .
Features
- Protection from –135°C to 150°C
- Superior thermal protection
- Water-resistant
- Comfortable / Lightweight / Excellent dexterity
- Breathable / Stay-dry lining.
Applications
- Ultra low and Blast freezers
- Cold rooms
- Dry ice handling
- Liquid Nitrogen, liquid oxygen, liquid helium
Environment
- Clinical Laboratory
- Bio-Medical
- Blood Banks
- Frozen food processing
- Pharmaceutical
| 71070 | Wrist Cryo-Gloves®, Small | 12" long | 1 pair | 122.00 | |
| 71071 | Wrist Cryo-Gloves®, Medium | 12" long | 1 pair | 122.00 | |
| 71072 | Wrist Cryo-Gloves®, Large | 12" long | 1 pair | 122.00 | |
| 71072-X | Wrist Cryo-Gloves®, X-Large | 12" long | 1 pair | 122.00 | |
| 71073 | Mid Arm Cryo-Gloves®, Small | 14" to 15" long | 1 pair | 132.00 | |
| 71074 | Mid Arm Cryo-Gloves®, Medium | 14" to 15" long | 1 pair | 132.00 | |
| 71075 | Mid Arm Cryo-Gloves®, Large | 14" to 15" long | 1 pair | 132.00 | |
| 71075-X | Mid Arm Cryo-Gloves®, X-Large | 14" to 15" long | 1 pair | 132.00 | |
| 71076 | Elbow Cryo-Gloves®, Small | 18" to 20" long | 1 pair | 155.00 | |
| 71076-M | Elbow Cryo-Gloves®, Medium | 18" to 20" long | 1 pair | 155.00 | |
| 71077 | Elbow Cryo-Gloves®, Large | 18" to 20" long | 1 pair | 155.00 | |
| 71078 | Elbow Cryo-Gloves®, X-Large | 18" to 20" long | 1 pair | 155.00 | |
| 71079-S | Shoulder Cryo-Gloves®, Small | 27" to 28" long | 1 pair | 197.00 | |
| 71079-M | Shoulder Cryo-Gloves®, Medium | 27" to 28" long | 1 pair | 197.00 | |
| 71079-L | Shoulder Cryo-Gloves®, Large | 27" to 28" long | 1 pair | 197.00 | |
| 71079-X | Shoulder Cryo-Gloves®, X-Large | 27" to 28" long | 1 pair | 197.00 | |
Cryo-Gloves® and Cryo-Aprons® are registered trademarks of Tempshield, Mt Desert ME.
Cryo-Apron®
These aprons are 100% water-proof with adjustable straps, for neck and waist.
| 71080 | Cryo-Apron®, Small 36" | each | 120.00 | |
| 71081 | Cryo-Apron®, Medium 42" | each | 130.00 | |
| 71082 | Cryo-Apron®, Large 48" | each | 142.00 | |
| 71082-X | Cryo-Apron®, X-Large 54" | each | 150.00 | |
Liquid
Nitrogen-Cooled Mortar
A stainless steel mortar is suspended by three brackets above a stainless steel bowl which holds the liquid nitrogen. The bowl is nested in a well insulated polyethylene housing. The 4"(102mm) insulated handle is removable for saving space. The housing measures 6½"(165mm) in diameter, 4½" high, and has three suction cup-feet. Now frozen tissue samples can be ground without the hazard of liquid nitrogen droplets flying into personnel
| 64470 | Liquid Nitrogen-Cooled Mortar | set | 265.00 | |
| 64471 | Spare mortar bowl | each | 89.00 | |
| 64472 | Ceramic Paste only, 180mm long | each | 30.00 | |
Cryogenic
Workstation
Corning® Cryogenic Workstation features a reusable cryogenic vial rack with a removable storage rack. Rack holds to 30 vials. The tray can be used to hold water or crushed ice. The racks and trays are made of autoclavable polycarbonate.
Overall measurement. 14½"(L)x6"(W)x3"(H) (216mmx184mmx83mm)
| 62040 | Cryogenic Workstation | each | 120.00 | |
| 62041 | Reusable Vial Rack Only | each | 92.00 | |
| 62042 | Reusable Storage Tray Only | each | 30.00 | |
Cryo Transport Box
Made from high impact polystyrene
- Ideal for transporting Cryo Vials
- Durable construction
- Organizes small amounts of Cryo Vials
- Holds 1.0 – 2.0ml Cryo Vials
| 62004-05 | Cryo Transport Box for 5 Tubes | 20/pk | 50.00 | |
| 62004-10 | Cryo Transport Box for 10 Tubes | 10/pk | 32.00 | |
Tissue-Tek® Cryomold®;
Sakura Finetek
These disposable plastic Cryomolds produce a uniformly shaped flat surface specimen block with tissue freezing medium (O.C.T.) for frozen sectioning, Standard Cryomold fits directly into Cryobar® freezing wells of the Tissue-Tek® II microtome cryostat. Use a Cryomold® adapter to adapt smaller biopsy and intermediate Cryomold® molds to freezing wells on the Cryobar® unit.
| Cat. No. | Tissue-Tek | Description | Pack | Price | |
| 62534-25 | 4557 | Standard, 25x20x5mm | 100/pk | 19.50 | |
| 62534-10 | 4565 | Biopsy, 10x10x5mm | 100/pk | 19.50 | |
| 62534-15 | 4566 | Intermediate 15x15x5mm | 100/pk | 19.50 | |
| 62535-15 | 4568 | Intermediate Cryomold Adapter | 12/pk | 28.00 | |
Tissue-Tek® Cryomold®;
Sakura Finetek
These disposable plastic Cryomolds produce a uniformly shaped flat surface specimen block with tissue freezing medium (O.C.T.) for frozen sectioning, Standard Cryomold fits directly into Cryobar® freezing wells of the Tissue-Tek® II microtome/cryostat. Use a Cryomold® adapter to adapt smaller biopsy and intermediate Cryomold® molds to freezing wells on the Cryobar® unit.
| Cat. No. | Tissue-Tek | Description | Pack | Price | |
| 62534-25 | 4557 | Standard, 25x20x5mm | 100/pk | 19.50 |  |
| 62534-10 | 4565 | Biopsy, 10x10x5mm | 100/pk | 19.50 | |
| 62534-15 | 4566 | Intermediate 15x15x5mm | 100/pk | 19.50 | |
Articles of Interest
Cryo Sectioning and the Use of Diamond Knives
