DiATOME Cryo Diamond Knife

The Low Temperature Ultra-Thin Collection:

Available for wet or dry sectioning with a 25°, 35° or a 45° angle.
Section thickness range 30nm - 1μm. Cutting edge sizes 1.5 - 4mm.

Diatome cryo diamond knives may be used for all low temperature situations such as sectioning of cryo-protected specimens, frozen hydrated specimens and industrial samples such as polymers and rubber.

There are numerous advantages to our cryo diamond knives as opposed to glass knives:

• They produce thinner sections.
• Ultrathin and semithin sections with the same knife.
• Easy to produce long ribbons of sections.
• Less compression and greater uniformity of sections.
• You gain time. No knife manufacturing, no knife changing, hence no waiting for temperature equilibration.

cryo 25°

The cryo 25° knife is designed for sectioning frozen hydrated specimens. The 25° angle results in the least possible compression and the best structure preservation.

cryo 35° cryo 45°

The 45° knife is well suited for routine cryo sectioning.

The 35° knife allows you to section with considerable reduction of mechanical damage to the specimen (up to 20% less compression as compared to the 45° knife) and, in turn, better structural preservation of most frozen samples (Ref 6, 7, 8).

The triangular holder for dry sectioning and the boat for the sectioning with a liquid (DMSO/water and others) are made of a special copper/nickel alloy. They both guarantee excellent cold or heat transmission.

The seal is made of a cold-resistant epoxy resin.

Styrene-butadiene block copolymer x 25'000, Ronald Walter, BASF Aktiengesellschaft, Polymer Physics, D-67056 Ludwigshafen.

Cryo Dry

Description	Angle	mm	Knife	ItemID	Price	Order
Cryo Dry Diamond Knife	25°	3.0mm	new	30-CD25	Price	Order
			resharpen	30-CD25R	Price	Order
Cryo Dry Diamond Knife	35°	1.5mm	new	15-CDL	Price	Order
			exchange	15-CDLE	Price	Order
			resharpen	15-CDLR	Price	Order
Cryo Dry Diamond Knife	35°	1.8mm	new	18-CDL	Price	Order
			exchange	18-CDLE	Price	Order
			resharpen	18-CDLR	Price	Order
Cryo Dry Diamond Knife	35°	2.1mm	new	21-CDL	Price	Order
			exchange	21-CDLE	Price	Order
			resharpen	21-CDLR	Price	Order
Cryo Dry Diamond Knife	35°	2.4mm	new	24-CDL	Price	Order
			exchange	24-CDLE	Price	Order
			resharpen	24-CDLR	Price	Order
Cryo Dry Diamond Knife	35°	2.5mm	new	27-CDL	Price	Order
			exchange	27-CDLE	Price	Order
			resharpen	27-CDLR	Price	Order
Cryo Dry Diamond Knife	35°	3.0mm	new	30-CDL	Price	Order
			exchange	30-CDLE	Price	Order
			resharpen	30-CDLR	Price	Order
Cryo Dry Diamond Knife	35°	3.5mm	new	35-CDL	Price	Order
			exchange	35-CDLE	Price	Order
			resharpen	35-CDLR	Price	Order
Cryo Dry Diamond Knife	35°	4.0mm	new	40-CDL	Price	Order
			exchange	40-CDLE	Price	Order
			resharpen	40-CDLR	Price	Order
Cryo Dry Diamond Knife	45°	1.5mm	new	15-CDS	Price	Order
			exchange	15-CDSE	Price	Order
			resharpen	15-CDSR	Price	Order
Cryo Dry Diamond Knife	45°	1.8mm	new	18-CDS	Price	Order
			exchange	18-CDSE	Price	Order
			resharpen	18-CDSR	Price	Order
Cryo Dry Diamond Knife	45°	2.1mm	new	21-CDS	Price	Order
			exchange	21-CDSE	Price	Order
			resharpen	21-CDSR	Price	Order
Cryo Dry Diamond Knife	45°	2.4mm	new	24-CDS	Price	Order
			exchange	24-CDSE	Price	Order
			resharpen	24-CDSR	Price	Order
Cryo Dry Diamond Knife	45°	2.5mm	new	27-CDS	Price	Order
			exchange	27-CDSE	Price	Order
			resharpen	27-CDSR	Price	Order
Cryo Dry Diamond Knife	45°	3.0mm	new	30-CDS	Price	Order
			exchange	30-CDSE	Price	Order
			resharpen	30-CDSR	Price	Order
Cryo Dry Diamond Knife	45°	3.5mm	new	35-CDS	Price	Order
			exchange	35-CDSE	Price	Order
			resharpen	35-CDSR	Price	Order
Cryo Dry Diamond Knife	45°	4.0mm	new	40-CDS	Price	Order
			exchange	40-CDSE	Price	Order
			resharpen	40-CDSR	Price	Order

Cryo Wet

Cryo Wet Diamond Knife	35°	1.5mm	new	15-CWL	Price	Order
			exchange	15-CWLE	Price	Order
			resharpen	15-CWLR	Price	Order
Cryo Wet Diamond Knife	35°	1.8mm	new	18-CWL	Price	Order
			exchange	18-CWLE	Price	Order
			resharpen	18-CWLR	Price	Order
Cryo Wet Diamond Knife	35°	2.1mm	new	21-CWL	Price	Order
			exchange	21-CWLE	Price	Order
			resharpen	21-CWLR	Price	Order
Cryo Wet Diamond Knife	35°	2.4mm	new	24-CWL	Price	Order
			exchange	24-CWLE	Price	Order
			resharpen	24-CWLR	Price	Order
Cryo Wet Diamond Knife	35°	2.5mm	new	27-CWL	Price	Order
			exchange	27-CWLE	Price	Order
			resharpen	27-CWLR	Price	Order
Cryo Wet Diamond Knife	35°	3.0mm	new	30-CWL	Price	Order
			exchange	30-CWLE	Price	Order
			resharpen	30-CWLR	Price	Order
Cryo Wet Diamond Knife	35°	3.5mm	new	35-CWL	Price	Order
			exchange	35-CWLE	Price	Order
			resharpen	35-CWLR	Price	Order
Cryo Wet Diamond Knife	35°	4.0mm	new	40-CWL	Price	Order
			exchange	40-CWLE	Price	Order
			resharpen	40-CWLR	Price	Order
Cryo Wet Diamond Knife	45°	1.5mm	new	15-CWS	Price	Order
			exchange	15-CWSE	Price	Order
			resharpen	15-CWSR	Price	Order
Cryo Wet Diamond Knife	45°	1.8mm	new	18-CWS	Price	Order
			exchange	18-CWSE	Price	Order
			resharpen	18-CWSR	Price	Order
Cryo Wet Diamond Knife	45°	2.1mm	new	21-CWS	Price	Order
			exchange	21-CWSE	Price	Order
			resharpen	21-CWSR	Price	Order
Cryo Wet Diamond Knife	45°	2.4mm	new	24-CWS	Price	Order
			exchange	24-CWSE	Price	Order
			resharpen	24-CWSR	Price	Order
Cryo Wet Diamond Knife	45°	2.5mm	new	27-CWS	Price	Order
			exchange	27-CWSE	Price	Order
			resharpen	27-CWSR	Price	Order
Cryo Wet Diamond Knife	45°	3.0mm	new	30-CWS	Price	Order
			exchange	30-CWSE	Price	Order
			resharpen	30-CWSR	Price	Order
Cryo Wet Diamond Knife	45°	3.5mm	new	35-CWS	Price	Order
			exchange	35-CWSE	Price	Order
			resharpen	35-CWSR	Price	Order
Cryo Wet Diamond Knife	45°	4.0mm	new	40-CWS	Price	Order
			exchange	40-CWSE	Price	Order
			resharpen	40-CWSR	Price	Order

References

A. Al-Amoudi, D. Studer and J. Dubochet: Cutting artefacts and cutting process in vitreous sections for cryo-electron microscopy. Journal of Structural Biology 150, pp. 109-121, 2005.

W. Liu, H. J. Geuze, J. W. Slot: Improving structural integrity of cryosections for immunogold labeling Histochemistry and Cell Biohgy, Vol. 106, PP. 41-55, 1996.

M. Michel, H. Gnagi and M. Muller: Diamonds are a cryosectioner's best friend. Journal of Microscopy, Vol 166, Pt 1, 43-56, 1992.

P. J. Peters: Cryo-lmmunogold Electron Microscopy, In Current Protocols in Cell Biology {J.S. Bonifacino, M. Dasso, J.B. Harford, J. Lippincott-Schwartz and K.M. Yamada, eds) pp. 4.7.1-4.7.12, 1999. Jonn Wiley & Sons, New York.

K. Richter: Cutting artifacts on ultrathin cryosections of biological bulk specimens. Micron, Vol. 25, No. 4, pp. 297-308. 1994.

P. Zhang, E. Bos, J. Heymann, H. Gnaegi, M. Kessel, P.J. Peters, S. Subramaniam: Direct visualisation of receptor arrays in frozen-hydrated sections and plungefrozen specimens of E.coli engineered to overproduce the chemotaxis receptor Tsr. Journal of Microscopy, Vol. 216, Pt 1, pp. 76-83, 2004.

What services can we offer you?

• Technical assistance in all fields of ultramicrotomy.
• Free sectioning tests for all types of samples.
• Make use of our many years of experience in perfecting our knives.
• With a telephone call, an e-mail or a fax message we can inform you of any details you require.

We are looking forward to hearing from you soon!

Your DiATOME Team

Cryo Immuno Diamond Knife