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Diatome Diamond Knives

Electron Microscopy Sciences

arrow14Histology Diamond Knives

arrowhisto and histo jumbo

Histo Diamond KnivesFor Room Temperature:

Knives are available with a 45° angle.

Section thickness range 0.2 - 10μm.

Cutting edge length 4, 5, 6, 8, and 10mm.

The knife is designed for the sectioning of hard and soft biological and industrial materials, non embedded or embedded in methacrylate or epoxy resins.

The histo knife may be used on all ultramicrotomes and microtomes with a retraction of the specimen in the return phase.

For the production of our histo knives we use natural diamonds of highest quality. They guarantee the best quality and durability of the cutting edges.

Some of the advantages of our histo diamond knife as compared to glass knives are as follows :

For 3D reconstruction it is imperative not to lose a single section (Ref. Blumer).

The large Jumbo boat as well as the adhesive (Pattex compact by Henkel) applied to the side of
the sample block increase the distinct advantages of our histo knives.

They allow:

diamond knife section of Nondecalcified rat bone
Nondecalcified rat bone., Scale: 35 mm = 100μm. Daniel Studer, Anatomisches Institut, Bern.

Histo Diamond Knivesarrowhisto - cryo

For Cryo Temperatures:

Knives are available in 45° angle.

Section thickness range 0.2 - 5μm.

Cutting edge length 4 and 6mm.

The histo-cryo knives are delivered in boats (for wet cryo sectioning) and triangular holders (for dry cryo sectioning), made of a special copper/nickel alloy, which guarantees the best heat/cold transmission.

DescriptionAnglemmKnifeItemIDPriceOrder
Histo Diamond Knife 45° 4.0mm new40-HIS$1,285.00Order
     resharpen40-HISR$700.00Order
Histo Diamond Knife 45° 6.0mm new60-HIS$1,950.00Order
     resharpen60-HISR$875.00Order
Histo Diamond Knife 45° 8.0mm new80-HIS$2,250.00Order
     resharpen80-HISR$1,200.00Order
Histo Cryo Diamond Knife 45° 4.0mm new40-HC$1,285.00Order
     resharpen40-HCR$700.00Order
Histo Cryo Diamond Knife 45° 6.0mm new60-HC$1,950.00Order
     resharpen60-HCR$875.00Order
Jumbo Histo Diamond Knife 45° 6.0mm new60-HISJ$2,100.00Order
     resharpen60-HISJR$975.00Order
Jumbo Histo Diamond Knife 45° 8.0mm new80-HISJ$2,300.00Order
     resharpen80-HISJR$1,275.00Order

 

diamond knife
E. B. Hunziker M. E. Müller Institute for Biomechanics,
University of Bern.
Rabbit joint, calcified cartilage/bone. X760 

References

O.L. Reymond: The «semi» diamond knife: a substitute for glass or conventional diamond knives in the ultramicrotomy of thin and semi-thin sections. Bas. Appl. Histochem. 30, pp. 487-494, 1986.

M.J.F. Blumer, P. Gahleitner, T. Narzt, C. Handl, B. Ruthensteiner: Strips of semi-thin sections: an advanced method with a new type of diamond knife. Journal of Neuroscience Methods 120,pp. 11-16, 2002. Contact adress M.J.F. Blumer: michael.blumer@uibk.ac.at

M.J.F. Blumer: Development of a unique eye: photoreceptors of the pelagic predator Atlanta peroni (Gastropoda, Heteropoda). Zoomorphology 119, pp. 81-91, 1996.

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Your DiATOME Team

AFM Diamond Knife for Ultra and Cryo arroearroe

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