Histology Diamond Knives

histo and histo jumbo

For Room Temperature:

Knives are available with a 45° angle.

Section thickness range 0.2 - 5μm.

Cutting edge length 4, 6, and 8mm.

The knife is designed for the sectioning of hard and soft biological and industrial materials, non embedded or embedded in methacrylate or epoxy resins.

The histo knife may be used on all ultramicrotomes and microtomes with a retraction of the specimen in the return phase.

For the production of our histo knives we use natural diamonds of highest quality. They guarantee the best quality and durability of the cutting edges.

Some of the advantages of our histo diamond knife as compared to glass knives are as follows :

• Perfect sections, free of scores or compression.
• Serial sections without knife change.
• Thinner sections.
• Extreme durability of the cutting edge.
• No knife making procedure.
• Lifetime Guarantee.

For 3D reconstruction it is imperative not to lose a single section (Ref. Blumer).

The large Jumbo boat as well as the adhesive (Pattex compact by Henkel) applied to the side of
the sample block increase the distinct advantages of our histo knives.

They allow:

• Easy production of section ribbons (0.5-2μm)
• No section loss
• No folding
• The same orientation of all sections
• Easy collection of section ribbons
• Multiple ribbons on one glass slide
• Perfect for Immuno-histo-chemistry

Nondecalcified rat bone., Scale: 35 mm = 100μm. Daniel Studer, Anatomisches Institut, Bern.

histo - cryo

For Cryo Temperatures:

Knives are available in 45° angle.

Section thickness range 0.2 - 5μm.

Cutting edge length 4 and 6mm.

The histo-cryo knives are delivered in boats (for wet cryo sectioning) and triangular holders (for dry cryo sectioning), made of a special copper/nickel alloy, which guarantees the best heat/cold transmission.

Description	Angle	mm	Knife	ItemID	Price	Order
Histo Diamond Knife	45°	4.0mm	new	40-HIS	Price	Order
			exchange	40-HISE	Price	Order
			resharpen	40-HISR	Price	Order
Histo Diamond Knife	45°	6.0mm	new	60-HIS	Price	Order
			exchange	60-HISE	Price	Order
			resharpen	60-HISR	Price	Order
Histo Diamond Knife	45°	8.0mm	new	80-HIS	Price	Order
			exchange	80-HISE	Price	Order
			resharpen	80-HISR	Price	Order
Histo Cryo Dry Diamond Knife	45°	4.0mm	new	40-HDC	Price	Order
			resharpen	40-HDCR	Price	Order
Histo Cryo Wet Diamond Knife	45°	4.0mm	new	40-HWC	Price	Order
			resharpen	40-HWCR	Price	Order
Histo Cryo Dry Diamond Knife	45°	6.0mm	new	60-HDC	Price	Order
			resharpen	60-HDCR	Price	Order
Histo Cryo Wet Diamond Knife	45°	6.0mm	new	60-HWC	Price	Order
			resharpen	60-HWCR	Price	Order
Jumbo Histo Diamond Knife	45°	6.0mm	new	60-HISJ	Price	Order
			exchange	60-HISJE	Price	Order
			resharpen	60-HISJR	Price	Order
Jumbo Histo Diamond Knife	45°	8.0mm	new	80-HISJ	Price	Order
			exchange	80-HISJE	Price	Order
			resharpen	80-HISJR	Price	Order

Description	Angle	mm	Knife	ItemID	Price	Order
Histo Diamond Knife	45°	4.0mm	new	40-HIS	Price	Order
			exchange	40-HISE	Price	Order
			resharpen	40-HISR	Price	Order
Histo Diamond Knife	45°	6.0mm	new	60-HIS	Price	Order
			exchange	60-HISE	Price	Order
			resharpen	60-HISR	Price	Order
Histo Diamond Knife	45°	8.0mm	new	80-HIS	Price	Order
			exchange	80-HISE	Price	Order
			resharpen	80-HISR	Price	Order
Histo Cryo Dry Diamond Knife	45°	4.0mm	new	40-HDC	Price	Order
			resharpen	40-HDCR	Price	Order
Histo Cryo Wet Diamond Knife	45°	4.0mm	new	40-HWC	Price	Order
			resharpen	40-HWCR	Price	Order
Histo Cryo Dry Diamond Knife	45°	6.0mm	new	60-HDC	Price	Order
			resharpen	60-HDCR	Price	Order
Histo Cryo Wet Diamond Knife	45°	6.0mm	new	60-HWC	Price	Order
			resharpen	60-HWCR	Price	Order
Jumbo Histo Diamond Knife	45°	6.0mm	new	60-HISJ	Price	Order
			exchange	60-HISJE	Price	Order
			resharpen	60-HISJR	Price	Order
Jumbo Histo Diamond Knife	45°	8.0mm	new	80-HISJ	Price	Order
			exchange	80-HISJE	Price	Order
			resharpen	80-HISJR	Price	Order

 

E. B. Hunziker M. E. Müller Institute for Biomechanics,
University of Bern.
Rabbit joint, calcified cartilage/bone. X760 

References

O.L. Reymond: The «semi» diamond knife: a substitute for glass or conventional diamond knives in the ultramicrotomy of thin and semi-thin sections. Bas. Appl. Histochem. 30, pp. 487-494, 1986.

M.J.F. Blumer, P. Gahleitner, T. Narzt, C. Handl, B. Ruthensteiner: Strips of semi-thin sections: an advanced method with a new type of diamond knife. Journal of Neuroscience Methods 120,pp. 11-16, 2002. Contact adress M.J.F. Blumer: michael.blumer@uibk.ac.at

M.J.F. Blumer: Development of a unique eye: photoreceptors of the pelagic predator Atlanta peroni (Gastropoda, Heteropoda). Zoomorphology 119, pp. 81-91, 1996.

What services can we offer you?

• Technical assistance in all fields of ultramicrotomy.
• Free sectioning tests for all types of samples.
• Make use of our many years of experience in perfecting our knives.
• With a telephone call, an e-mail or a fax message we can inform you of any details you require.

We are looking forward to hearing from you soon!

Your DiATOME Team

AFM Diamond Knife for Ultra and Cryo