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Electron Microscopy Sciences

Diatome Diamond Knives

PDF Downloadarrow13DiATOME Ultra Diamond Knife

Ultra Diamond KnifeThese knives are available in 35, and 45 degree angles. The optimal thickness range for cutting with these knives is between 30-150nm. They are available in sizes ranging from 1.5mm-4.0mm as a standard. Other sizes are available upon request. All of these knives are tested within a thickness range of 30- 150nm. Whether your needs are biological or materials related, in EM or LM, we have a knife to suit your needs.

arrowUltra 45°

Recognized as the knife for routine sectioning. It is a good compromise between section quality and durability.

The 45° knife is recommended for the sectioning of extremely hard and brittle materials such as ceramics. In this domain it has replaced the formerly recommended 55° knife.

arrowUltra 35° Wet

The capabilities of the 35° diamond knives have been shown in two publications by J.Jésior (Ref 3, 4). He proved markedly reduced compression, smoother section surfaces and better structural preservation with the use of our 35° diamond knives.

In the meantime a great number of scientists have noticed and recognized the advantages of the 35° knives for the sectioning of Lowicryls, inhomogenous samples such as undecalcified bone, dental materials, etc.

The 35° knives have proved advantageous in the sectioning of soft industrial samples such as metals and polymers, as well as for hard and brittle samples, for example semiconductors (Si, GaAs, etc), superconducting oxides, nanocrystalline ceramics.

The use of 35° knives leads to reduced compression in soft samples and to less breaking in hard and brittle samples.

We can claim that the 35° diamond knife has proved it's capability as a routine knife for many applications.

ultra diamond knife
Rat muscle (Quadriceps) x 23’000
Werner Graber, Anatomisches Institut, Bern

arrowUltra-Semi 35°

Similar to our ultra 35° 3.0 mm with a greater thickness range (50-500nm)

ultra 35 dryarrowUltra 35° Dry

The ultra 35° knife (in the triangular holder) with a cutting range of 100nm - 2µm is used for dry sectioning of epoxy or acrylic resin embedded biological samples, which need to be investigated by element analysis (Ref. Edelmann) and SIMS (Ref. Guerquin-Kern). The gliding of the sections on the dry knife surface is facilitated with the use of our Static Line II ionizer.

arrowUltra Diamond knifeUltra Jumbo

The Ultra Diamond knife is now available in Jumbo with 35° and 45° angles and in 2.0mm and 3.0mm sizes.

Ordering:

DescriptionAnglemmKnifeCat#PriceOrder
Ultra Diamond Knife - Wet35° 1.5mm new15-UL 1,995.00Add to Cart
      exchange15-ULE 1,350.00Add to Cart
      resharpen15-ULR 995.00Add to Cart
Ultra Diamond Knife - Dry 35°  1.5mm  new 15-ULD 1,995.00 Add to Cart
      exchange 15-ULDE 1,350.00 Add to Cart
      resharpen 15-ULDR 995.00 Add to Cart
Ultra Diamond Knife - Wet35° 1.8mm new18-UL 2,400.00Add to Cart
      exchange18-ULE 1,450.00Add to Cart
      resharpen18-ULR 1,200.00Add to Cart
Ultra Diamond Knife - Dry 35°  1.8mm  new 18-ULD 2,400.00 Add to Cart
      exchange 18-ULDE 1,450.00 Add to Cart
      resharpen 18-ULDR 1,200.00 Add to Cart
Ultra Jumbo Diamond Knife 35° 2.0mm new20-ULJ 3,300.00Add to Cart
   resharpen 20-ULJR 1,400.00 Add to Cart
Ultra Diamond Knife - Wet35° 2.1mm new21-UL 2,418.00 Add to Cart
      exchange21-ULE 1,695.00 Add to Cart
      resharpen21-ULR 1,390.00 Add to Cart
Ultra Diamond Knife - Dry 35°  2.1mm  new 21-ULD 2,418.00 Add to Cart
      exchange 21-ULDE 1,695.00 Add to Cart
      resharpen 21-ULDR 1,390.00 Add to Cart
Ultra Diamond Knife - Wet35° 2.4mm new24-UL 2,800.00 Add to Cart
   exchange24-ULE 1,900.00 Add to Cart
      resharpen24-ULR 1,595.00 Add to Cart
Ultra Diamond Knife - Dry 35°  2.4mm  new 24-ULD 2,800.00 Add to Cart
      exchange 24-ULDE 1,900.00 Add to Cart
      resharpen 24-ULDR 1,595.00 Add to Cart
Ultra Diamond Knife - Wet35° 2.5mm new27-UL 2,800.00 Add to Cart
      exchange27-ULE 1,940.00 Add to Cart
      resharpen27-ULR 1,675.00 Add to Cart
Ultra Diamond Knife - Dry 35°  2.5mm  new 27-ULD 2,800.00 Add to Cart
      exchange 27-ULDE 1,940.00 Add to Cart
      resharpen 27-ULDR 1,675.00 Add to Cart
Ultra Jumbo Diamond Knife 35° 3.0mm new30-ULJ 4,275.00 Add to Cart
      resharpen30-ULJR 1,975.00 Add to Cart
Ultra Diamond Knife - Wet35° 3.0mm new30-UL 3,285.00 Add to Cart
      exchange30-ULE 2,300.00 Add to Cart
      resharpen30-ULR 1,985.00 Add to Cart
Ultra-Semi Diamond Knife 35°  3.0 mm new 30-SL 3,285.00 Add to Cart
      exchange 30-SLE 2,300.00 Add to Cart
      resharpen 30-SLR 1,985.00 Add to Cart
Ultra Diamond Knife - Dry 35°  3.0mm  new 30-ULD 3,285.00 Add to Cart
      exchange 30-ULDE 2,300.00 Add to Cart
      resharpen 30-ULDR 1,985.00 Add to Cart
Ultra Diamond Knife - Wet35° 3.5mm new35-UL 3,680.00 Add to Cart
      exchange35-ULE 2,630.00 Add to Cart
      resharpen35-ULR 2,300.00 Add to Cart
Ultra Diamond Knife - Dry 35°  3.5mm  new 35-ULD 3,680.00 Add to Cart
      exchange 35-ULDE 2,630.00 Add to Cart
      resharpen 35-ULDR 2,300.00 Add to Cart
Ultra Diamond Knife - Wet35° 4.0mm new40-UL 4,085.00 Add to Cart
      exchange40-ULE 2,900.00 Add to Cart
      resharpen40-ULR 2,550.00 Add to Cart
Ultra Diamond Knife - Dry 35°  4.0mm  new 40-ULD 4,085.00 Add to Cart
      exchange 40-ULDE 2,900.00 Add to Cart
      resharpen 40-ULDR 2,550.00 Add to Cart
Ultra Diamond Knife - Wet45° 1.5mm new15-US 1,995.00 Add to Cart
      exchange15-USE 1,350.00 Add to Cart
      resharpen15-USR 995.00 Add to Cart
Ultra Diamond Knife - Wet45° 1.8mm new18-US 2,400.00 Add to Cart
      exchange18-USE 1,450.00 Add to Cart
      resharpen18-USR 1,200.00 Add to Cart
Ultra Jumbo Diamond Knife 45° 2.0mm new20-USJ 3,300.00 Add to Cart
      resharpen20-USJR 1,400.00 Add to Cart
Ultra Diamond Knife - Wet45° 2.1mm new21-US 2,418.00 Add to Cart
      exchange21-USE 1,695.00 Add to Cart
      resharpen21-USR 1,390.00 Add to Cart
Ultra Diamond Knife - Wet45° 2.4mm new24-US 2,800.00 Add to Cart
      exchange24-USE 1,900.00 Add to Cart
      resharpen24-USR 1,595.00 Add to Cart
Ultra Diamond Knife - Wet45° 2.5mm new27-US 2,800.00 Add to Cart
      exchange27-USE 1,940.00 Add to Cart
      resharpen27-USR 1,675.00 Add to Cart
Ultra Jumbo Diamond Knife 45° 3.0mm new30-USJ 4,200.00 Add to Cart
      resharpen30-USJR 1,995.00 Add to Cart
Ultra Diamond Knife - Wet45° 3.0mm new30-US 3,285.00 Add to Cart
      exchange30-USE 2,300.00 Add to Cart
      resharpen30-USR 1,985.00 Add to Cart
Ultra Diamond Knife - Wet45° 3.5mm new35-US 3,680.00 Add to Cart
      exchange35-USE 2,630.00 Add to Cart
      resharpen35-USR 2,300.00 Add to Cart
Ultra Diamond Knife - Wet45° 4.0mm new40-US 4,085.00 Add to Cart
      exchange40-USE 2,900.00 Add to Cart
      resharpen40-USR 2,550.00 Add to Cart

References

J.C. Jésior: Use of low-angle diamond knives leads to improved ultrastructural preservation of ultrathin sections. Scanning Microscopy Supplement 3, pp. 17-153, 1989. Scanning Microscopy International,
Chicago (AMF O’Hare) IL 6066 USA.

L. Edelmann: Freeze-substitution and the preservation of diffusable ions. Journal of Microscopy, Vol. 161, pp. 217-228, 1991.

G. Mahon and T. Malis: Ultramicrotomy of Nano-crystalline Materials. Microscopy Research and Technique, Vol. 31, pp. 267-274, 1995.

S.R. Glanvill: Ultramicrotomy of Semiconductors and Related Materials. Microscopy Research and Technique, Vol. 31, pages 267-274, 1995.

P. Swab abd R.E. Klinger: Preparation of multilayer coatings for cross-sectional Microanalysis by Ultramicrotomy. Mat. Res. Soc. Symp. Proc. Vol. 115, pages 229-234, 1989.

P. Swab: Ultra-microscopy of Diamond Films for TEM Cross-Section Analysis. Microscopy Research and Technique, Vol. 31, pp. 308-310, 1995.

C. Quintana: Ultramicrotomy for Cross-sections of Nanostructure. Micron Vol. 28, No. 3, pages 217-219, 1997.

Y. Maniette: Microtomy, a convenient method for preparing TEM samples in ceramic science. Journal of Material Science Letters 9, pages 48-50, 1990.

P. Schubert-Bischoff and T. Krist: Fast cross-sectioning technique for thin films by Ultramicrotomy. Microscopy and Microanalysis, proceedings, page 359, 1997.

J.L. Guerquin-Kern, T.D. Wu, C. Quintana, A. Croisy: Progress in analytical imaging of the cell by dynamic secondary ion mass spectroscopy (SIMS microscopy). BBA 1724, pp. 228-238, 2005.

What services can we offer you?

  • Technical assistance in all fields of ultramicrotomy.
  • Free sectioning tests for all types of samples.
  • Make use of our many years of experience in perfecting our knives.
  • With a telephone call, an e-mail or a fax message we can inform you of any details you require.

We are looking forward to hearing from you soon!

Your DiATOME Team

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