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Embedding Media Kits

Electron Microscopy Sciences

arrow14Methyl Methacrylate and Glycol Methacrylate

Technovit Methyl Methacrylate and Glycol Methacrylatearrow12Technovit® 9100

Newly introduced! Technovit® 9100 is an embedding medium system based on methyl methacrylate (MMA), which hardens at low temperature. It is designed for the embedding of mineralized tissues with extensive possibilities of staining for light microscopy.

Applications:

  • Heavy duty microtome technique for preparing thin sections – such as pelvic biopsy, small low and high density bone samples.
  • Cutting grinding and micro-grinding system (contact point technique) - such as jaw and teeth segments with and without implant.
  • Combined contact point and heavy duty microtome technique (target preparation) - such as interface regions and examination of tissues surrounding metal implants and cement-free endoprothesis (target tissue preparation).

Properties:

Technovit® 9100 Polymerization takes place under the exclusion of oxygen with the help of a peroxide/amine catalyst. Additional components such as PMMA-powder and a regulator allows for a controlled polymerization at temperatures between –8 and –20°C, which guarantees for the dispersion of the heat generated during polymerization. The polymerization time is between 18 and 24 hours using a volume between 3 and 15 ml in the above temperature range. All the routine techniques such as preparation tissues, staining, immuno- and enzyme-histochemistry, in-situ hybridization techniques can be performed.

  • Basic Solution – is composed of organic monomers, with at least one carbon-carbon double bond (C=C). The stabilizer added to this solution is for storage-stability. Hydrophilic properties are improved by the addition of a special hydrophilic generating agent.
  • PMMA-Powder – is an internal filler and it is made up of PMMAmicro pellets. It is used to:
    - Reduce the polymerization shrinking effect.
    - Reduce the heat generated during the polymerization process.
    - Improve the quality of the polymerization block.
  • Hardener 1 – is one of the components of the polymerization initiation system. It is a derivative of dibenzoyl peroxide, which in combination with Hardener 2, makes the polymerization take place.
  • Hardener 2 – it works catalytically upon hardener 1 to allows for a controlled polymerization, even at temperatures below 0°C.
  • Regulator – is composed of a reactive organic compound, which allows a controlled polymerization, even with large volumes of polymer, without increases in the temperature during the polymerization reaction.
  • PMMA-Granulate, EXART® - this granulate acts as an additional internal filler when larger amounts (500-1000ml) of polymer are to be used, for example, in the case of femur shaft with noncemented endoprosthses. The amount of monomer (basic solution) is thereby reduced, at the same time making the polymerization easier to control.
RT 14655 Kit consists of:
1000ml Basic Solution (stabilized)
120g PMMA-Powder
8 Bags @ 1g Hardener 1
10ml Hardener 2
5ml Polymerization Regulator
500gPMMA-Granulate, EXART*
Kit 208.00 cart
  10400 Aluminum Oxide 500 gram 60.00 cart

*EXART – Trade mark of EXART-Apparratebau GmbH & Co. KG, D-22851 Norderstedt

Technovit® 7100 and 8100 Embedding Kits arrow12Technovit® 7100 and 8100 Embedding Kits

Technovit® Glycol Methacrylate embedding kits developed by Kulzer in Germany are the most convenient to use, and offers the best embedding results.

Features:

  • Technovit® GMA kits give better results offering improved morphology,
    and allows for 1 micron serial sectioning.
  • Technovit® 7100 GMA is less sensitive to O2 during polymerization
    so sealing of the molds is not necessary.
  • The polymerization temperature never exceeds 40°C, which
    makes either kit a potential choice for enzyme histochemistry.
    Initiation chemistry of Technovit® 8100 GMA makes polymerization
    at 4°C possible for improved signal during immunohistochemistry.
  • The initiator is prepackaged, eliminating messy handling of dry
    components, and by eliminating aromatic amines from the formation,
    the components are significantly less toxic.
  • The consistent high quality of raw materials used to make the
    resin allows for a permanent clear colorless block and, most
    important, reproducible results.
RT 14653 Technovit® H7100 –GAM kit consists of:
500ml Glycol Methacrylate monomer
5 x 0.6g packs Hardener I
40ml Hardener II
Kit 145.00 cart
RT 14654 Technovit® H8100 – GMA kit consists of:
500ml Glycol Methacrylate Monomer
5 x 0.6g packs Hardener I
30ml Hardener II
Kit 205.00 cart

Technovit H7100/H8100

arrow12Technovit® 3040 – Mounting Medium

Technovit 3040 yellow is a fast curing methyl methacrylate-based resin, whose chemical composition warrants a firm, durable bond between Technovit and the specimen.

Technovit® 3040 consists of two components – powder and liquid – allowing simple mixing, easy adherance to the specimen, and fast curing. For fixing the mount, a high viscous consistency is required(i.e. a mixing ratio of approximately 2-3 parts per volume powder: 1 part
per volume liquid) has proven to be the most advantageous.

RT 14652 Technovit® 3040 Kit consists of:
1 x 100g Technovit® 3040 powder
1 x 80ml Technovit™ 3040 liquid
Kit 75.00 cart

arrow12Technovit 4000

The low Shrinkage Embedding Medium

Technovit 400 is a fast curing, cold polymerizing, three-component resin, which is based on a modified polyester, and it is available in the form of powder, syrup I, and syrup II. It is mixed at a ratio of 2:2:1 (powder:syrup I:syrup II). Syrup I and Syrup II are mixed together first and the powder is mixed in last. The color is white opaque.

Properties:

Technovit 4000 is distinguished by its low shrinkage when polymerizing and its perfect margin fit. Due to its excellent flow characteristics, Technovit 4000 guaranteed that geometrically demanding samples are optimally embedded. Its excellent adhesion properties with regard to
metal are a guarantee of gapless embedding of all metal samples. These properties are of particular importance when working with samples that require good edge definitions.

  • Excellent margin fit
  • Optimum grinding and polishing properties

Applications:

After mixing, Technovit 4000 can be used for casting for approximately 4 minutes and takes approximately 8 minutes to cure.

Ordering:

RT 14656-01 Technovit 4000 Small Kit
20 g Powder, 60 ml Liquid I, and 40 ml Liquid II
Kit 64.00 cart
RT 14656-02 Technovit 4000 Medium Kit
750 g Powder, 500 ml Liquid I, and 250 ml Liquid II
Kit 124.00 cart
RT 14656-03 Technovit 4000 Large Kit
1500 g Powder, 1000 ml Liquid I, and 500 ml Liquid II
Kit 192.00 cart

arrow12Conductance Embedding Media

A two component resin based on modified methyl methacrylate and conducting carbon powder. The polymerized resin gives excellent adhesion to the embedded material and homogeneous conductivity. This embedding resin is good for SEM, microprobe specimen, and specimens to be electro polished. The kit consists of 1kg of component A Powder and 500 ml components B Liquid.

  13960 Conductance Embedding Kit 1 kilo 175.00 cart

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