Methyl Methacrylate and Glycol Methacrylate
Technovit® by Kulzer: The Resins and Accessories for all Methacrylate Embedding
Technovit is one of the most sort after named Methacrylate embedding medias in the world due to its diversity and availability of different kits and properties there is a media for each of your applications.
Features:
- Technovit GMA kits give better results with improved morphology, and the plastic makes serial 1µ sectioning possible.
- Technovit 7100 GMA is less sensitive to O2 during polymerization so sealing of the molds is not necessary.
- The polymerization temperature never exceeds 40°C, making either Technovit kit a potential choice for enzyme histochemistry. Initiation chemistry of Technovit 8100 GMA makes polymerization at 4°C possible for improved signal during immunohistochemistry.
- The initiator is prepackaged, eliminating messy handling of dry components. And, by eliminating aromatic amines from the formulation, the components are significantly less toxic.
- The constantly high quality of raw materials gives permanently clear, colorless blocks and more reproducible results.
Technical Note:
GMA resin-based products (Technovit® 7100, 8100) should not be used when epoxy removal from specimens is required. There is no known method of GMA epoxy removal (including methoxide) that does not effectively render specimens unusable. Instead, MMA resin-based products such as Technovit® 9100 should be selected.
Technovit® 9100
Newly introduced! Technovit® 9100 is an embedding medium system based on methyl methacrylate (MMA), which hardens at low temperature. It is designed for the embedding of mineralized tissues with extensive possibilities of staining for light microscopy.
Applications:
- Heavy duty microtome technique for preparing thin sections – such as pelvic biopsy, small low and high density bone samples.
- Cutting grinding and micro-grinding system (contact point technique) - such as jaw and teeth segments with and without implant.
- Combined contact point and heavy duty microtome technique (target preparation) - such as interface regions and examination of tissues surrounding metal implants and cement-free endoprothesis (target tissue preparation).
Properties:
Technovit® 9100 Polymerization takes place under the exclusion of oxygen with the help of a peroxide/amine catalyst. Additional components such as PMMA-powder and a regulator allows for a controlled polymerization at temperatures between –8 and –20°C, which guarantees for the dispersion of the heat generated during polymerization. The polymerization time is between 18 and 24 hours using a volume between 3 and 15 ml in the above temperature range. All the routine techniques such as preparation tissues, staining, immuno- and enzyme-histochemistry, in-situ hybridization techniques can be performed.
Basic Solution – is composed of organic monomers, with
at least one carbon-carbon double bond (C=C). The stabilizer added
to this solution is for storage-stability. Hydrophilic properties
are improved by the addition of a special hydrophilic generating
agent.- PMMA-Powder – is an internal filler and it is made up
of PMMAmicro pellets. It is used to:
- Reduce the polymerization shrinking effect.
- Reduce the heat generated during the polymerization process.
- Improve the quality of the polymerization block.
- Hardener 1 – is one of the components of the polymerization
initiation
system. It is a derivative of dibenzoyl peroxide, which in combination
with Hardener 2, makes the polymerization take place.
- Hardener 2 – it works catalytically upon hardener
1 to allows for
a controlled polymerization, even at temperatures below 0°C.
- Regulator – is composed of a reactive organic compound, which allows a controlled polymerization, even with large volumes of polymer, without increases in the temperature during the polymerization reaction.
- PMMA-Granulate, EXART® - this granulate acts as an additional internal filler when larger amounts (500-1000 ml) of polymer are to be used, for example, in the case of femur shaft with noncemented endoprosthses. The amount of monomer (basic solution) is thereby reduced, at the same time making the polymerization easier to control.
| RT | 14655 | Kit consists of: 1000 ml Basic Solution (stabilized) 120g PMMA-Powder 8 Bags @ 1g Hardener 1 10 ml Hardener 2 5 ml Polymerization Regulator 500gPMMA-Granulate, EXART* |
Kit | 212.00 | Add to Cart |
| 10400 | Aluminum Oxide 90 Basic (63-200 Micron) | 500 gram | 150.00 | Add to Cart |
*EXART – Trade mark of EXART-Apparratebau GmbH & Co. KG, D-22851 Norderstedt
Technovit® 7100 and 8100 Embedding Kits
Technovit® Glycol Methacrylate embedding kits developed by Kulzer in Germany are the most convenient to use, and offers the best embedding results.
Features:
- Technovit® GMA kits give better results
offering improved morphology,
and allows for 1 micron serial sectioning. - Technovit® 7100 GMA is less sensitive to O2 during polymerization
so sealing of the molds is not necessary. - The polymerization temperature never exceeds 40°C, which
makes either kit a potential choice for enzyme histochemistry.
Initiation chemistry of Technovit® 8100 GMA makes polymerization
at 4°C possible for improved signal during immunohistochemistry. - The initiator is prepackaged, eliminating messy handling
of dry
components, and by eliminating aromatic amines from the formation,
the components are significantly less toxic. - The consistent high quality of raw materials used to make
the
resin allows for a permanent clear colorless block and, most
important, reproducible results.
| RT | 14653 | Technovit® H7100 –GAM
kit consists of: 500 ml Glycol Methacrylate monomer 5 x 0.6g packs Hardener I 40 ml Hardener II |
Kit | 150.00 | Add to Cart |
| RT | 14654 | Technovit® H8100 – GMA
kit consists of: 500 ml Glycol Methacrylate Monomer 5 x 0.6g packs Hardener I 30 ml Hardener II |
Kit | 212.00 | Add to Cart |
Technovit H7100/H8100
- Staining Protocol for Lymphoid Tissue
- Sectioning and Mounting Protocol
- Prussian Blue Stain for Glycol Methacrylate Sections
- Periodic Acid Schiff Stain for Glycol Methacrylate Sections
- Hematoxylin and Eosin Stain for Glycol Methacrylate Sections
- Glycol Methacrylate Embedding for Materials Samples
- Giemsa Stain For Glycol Methacrylate Sections
- Glycol Methacrylate Embedding for Soft Tissues
- Glycol Methacrylate Embedding For Immunohistochemistry
- Alkaline Phosphatase for Glycol Methacrylate Sections
- Acid Phosphatase for Glycol Methacrylate Section
Technovit® Glycol Methacrylate 8100 Histo Techniques Set
This unique set includes:
- Technovit Glycol Methacrylate Kit 8100 with 500 ml of GMA monomer, 5 - 0.6 g packs of Hardener I, and 30 ml of Hardener II
- 500 cover foils
- 100 pieces Histobloc
- 1 piece Histoform S or Q Embedding Mold
- 100 piece Histoknife H
- 1 piece microtome knife holder, 17 or 22 cm
- Technovit 3040 Kit - Technovit 3040, 100g powder and 80 ml liquid
- dosing devices
| RT | 14654-25 | Technovit 8100 Histo Set | Kit | 1995.00 | Add to Cart |
Technovit® Glycol Methacrylate 7100 Histo Techniques Set
This unique set includes:
- Technovit Glycol Methacrylate Kit 7100 with 500 ml of GMA monomer, 5 x 1g packs of Hardener I, and 40 ml of Hardener II
- 100 pieces Histobloc
- 1 piece Histoform S or Q Embedding Mold
- 100 piece Histoknife H
- 1 piece microtome knife holder, 17 or 22 cm
- Technovit 3040, 100g powder and 80 ml liquid
- dosing devices
| RT | 14654-26 | Technovit 7100 Histo Set | Kit | 1995.00 | Add to Cart |
Technovit® 3040 –
Mounting Medium
Technovit 3040 yellow is a fast curing methyl methacrylate-based resin, whose chemical composition warrants a firm, durable bond between Technovit and the specimen.
Technovit® 3040 consists of two components – powder and liquid –
allowing simple mixing, easy adherance to the specimen, and fast curing. For fixing the mount, a high viscous consistency is required(i.e. a mixing ratio of approximately 2-3 parts per volume
powder: 1 part
per volume liquid) has proven to be the most advantageous.
| RT | 14652 | Technovit® 3040 Kit consists of: 1 x 100g Technovit® 3040 powder 1 x 80 ml Technovit™ 3040 liquid |
Kit | 78.00 | Add to Cart |
Technovit 4000
The low Shrinkage Embedding Medium
Technovit 400 is a fast curing, cold polymerizing, three-component resin, which is based on a modified polyester, and it is available in the form of powder, syrup I, and syrup II. It is mixed at a ratio of 2:2:1 (powder:syrup I:syrup II). Syrup I and Syrup II are mixed together first and the powder is mixed in last. The color is white opaque.
Properties:
Technovit 4000 is distinguished by its low shrinkage when polymerizing
and its perfect margin fit. Due to its excellent flow characteristics,
Technovit 4000 guaranteed that geometrically demanding samples are
optimally embedded. Its excellent adhesion properties with regard to
metal are a guarantee of gapless embedding of all metal samples. These
properties are of particular importance when working with samples that
require good edge definitions.
- Excellent margin fit
- Optimum grinding and polishing properties
Applications:
After mixing, Technovit 4000 can be used for casting for approximately 4 minutes and takes approximately 8 minutes to cure.
Ordering:
| RT | 14656-02 | Technovit 4000 Medium Kit 750 g Powder, 500 ml Liquid I, and 250 ml Liquid II |
Kit | 136.00 | Add to Cart |
| RT | 14656-03 | Technovit 4000 Large Kit 1500 g Powder, 1000 ml Liquid I, and 500 ml Liquid II |
Kit | 192.00 | Add to Cart |
Technovit® Embedding Molds
An important prerequisite for easy-to-cut specimens is a mold with a special profile which will provide specimen blocks for easy application and simple cutting. This is achieved with Histoform S, an embedding mold composed of new material with a special shape.
All essential requirements such as:
- Material-saving volume
- Easy-to-cut shape
- Unlimited shelf life
- Easy removal of the embedded specimens because of the conical shape and the Teflon® material
- Excellent temperature dissipation by way of refined steel bottom
- Accurate and easy fixing of the specimen blocks byspecial grooves were achieved with Histoform S, in cooperation with renowned users.
Curing temperature measurements have confirmed that at this volume the combination of Teflon and refined steel will result in a considerably lower polymerization temperature.
Three types types are available: the Histoform Q and the Histoform S and the Histoform N. The Histoform Q was developed for the embedding of larger specimens; the characteristics are identical to Histoform S. These PTFE molds allow for easy removal of 10 plastic blocks when using the Histobloc system.
The mold cavities measure 10mm wide x 16mm high x 6.5mm deep in th "S" and 20mm wide x16mm high x10mm deep in the "Q" and in the "N" 9mm W x 18mm H x 5mm D.
| RT | 14654-30 | HistoForm Q | each | 775.00 | Add to Cart |
| RT | 14654-35 | HistoForm S | each | 700.00 | Add to Cart |
| RT | 14654-40 | HistoForm N | each | 750.00 | Add to Cart |
Technovit® HistoBloc
Due to the special demand for the thinnest possible sections, polymerized Technovit® 7100 is most elastic and it is therefore recommended to fix the embedded specimen on a universal stable mount, to insure optimum clamping and cutting.
To avoid a subsequent complicated removal of the specimens from the mount, an inexpensive disposable version was developed.
Features:
- Fixed easily, securely and permanently
- Clamped into all rotary microtomes without adapter
- Embedded in the elastic Technovit in such a way that thinnest sections may be cut
- Optimally fixed using Technovit® 3040 at low cost(polymerisation in 5 to 10 minutes)
- Easy to write on
- Link together for storage
| RT | 14654-60 | HistoBloc for "S" and "Q" | 100/pk | 99.50 | Add to Cart |
| RT | 14654-70 | HistoBloc for "N" | 50/pk | 22.00 | Add to Cart |
Additional Technovit® General Accessories and Supplies
| RT | 14653-11 | Microtome Knife Holder 17CM | each | 1218.00 | Add to Cart |
| RT | 14653-12 | Microtome Knife Holder 22CM | each | 1500.00 | Add to Cart |
| RT | 14653-13 | Histo Knife H | 30/pk | 160.00 | Add to Cart |
| RT | 14653-14 | Histo Knife H | 100/pk | 450.00 | Add to Cart |
| RT | 14653-15 | Mounting Press | each | 550.00 | Add to Cart |
| RT | 14653-16 | PE -Foils 75x25mm | each | 14.00 | Add to Cart |
| RT | 14653-17 | Insert For Specimen Holder M | each | 325.00 | Add to Cart |
| RT | 14653-18 | Specimen Holder H | each | 1300.00 | Add to Cart |
| RT | 14653-19 | Embedding Mold 25mm | 3/pk | 85.00 | Add to Cart |
| RT | 14653-20 | Insert for Embedding Form 25mm | 3/pk | 38.00 | Add to Cart |