Unicryl
and Vestopal
Unicryl
™
A New Universal Resin for:
Light Microscopy- Electron Microscopy
- Immunolabeling
- In-Situ Hybridization
- Histochemistry
UNICRYL is a single component and easy to use resin which gives excellent structural preservation of tissues without chemically interacting or crosslinking with them. UNICRYL is largely hydro-philic, allowing good access to polar solutions and exhibiting low background staining or labeling from hydrophobic materials. It also minimizes the denaturation of proteins, allowing true antigenic properties to be maintained.
The excellent polymerization properties of UNICRYL are largely due to the fact that all of the components of the polymer have similar molecular weights, ensuring even penetration into the tissue.
In addition, the enhanced labeling, staining and hybridizing qualities arise from the fact that sections are cleaved from the block face ahead of the knife edge, thus exposing more of the tissue components at the surface. UNICRYL is premixed and supplied as a single component. The resin has a long shelf life when stored in the cold. It is miscible with alcohol and has a low viscosity even down to -50°C. The resin can be polymerized by heat (50-60°C) or by UV irradiation at low temperatures (-10 to 20°C).
The polymerization of the resin is an exothermic reaction. Unicryl shrinks approximately 10% in volume during polymerization. Ethyl alcohol, acetone and many other solvents may be used as a dehydrating agent.
Complete instructions come with the kit.
| 0-5°C | 14660 | Unicryl kit consists of: 250 ml Unicryl Resin 1 pack of 1ml embedding vials 1 pair plastic gloves 1 plastic bag for disposal 1 waste container Unicryl Embedding Kit |
each Kit | 248.00 |  |
Vestopal ® 310
This resin has a fine grain and sections are easy to stain. It
is characterized by rapid penetration and polymerization and does not
show uneven polymerization. It hardens without shrinkage and the sections
are stable under the electron beam.
Ryter, et al., J. Ultrastruct. Res., 2:200 (1958)
| RT | 14700 | Kit consists of: 450ml Vestopal 310 (Formerly W) 10ml Cobalt napthenate 25ml t-Butyl perbenzoate |
Kit | 35.00 | |
Technical Tips
Rapid Techniques for Specimen Preparation
