A.1 BACTERIAL STAINS

Truant’s Fluorescent Method for Acid Fast Organism

For bacterial stain.

Stain results:

Acid Fast:		Fluoresce reddish yellow
Artifacts:		Fluoresce yellow

References:

• Truant, J.P. Henry Ford Hospital Med. Bull., 10:287, 1962.
• AFIP Manual of Histological Staining Methods, 3rd ed. Ed. L. Luna: New York: McGraw Hill Publications. c. 1968. p. 219.
• Clark, G.: Staining Procedures. Williams and Wilkens Co., Baltimore. 3rd edition. c.1973. p. 225

RT	26102-1A	Weigert’s Iron Hematoxylin Solution A	500ml	44.00	Add to Cart
RT	26102-1B	Weigert’s Iron Hematoxylin Solution B	500ml	32.00	Add to Cart
RT	26102-02	Auramine-Rhodamine Staining Solution	250ml	58.00	Add to Cart
RT	26102-03	Acid Alcohol 1%	500ml	22.00	Add to Cart

Brown and Brenn Method for Gram Positive and Gram Negative Bacteria

For bacteria stain: Gram + / Gram – bacteria and filaments of nocardia, etc.

Stain results:

Gram + Bacteria, Nocardia and Actinomyces Filaments:		Blue
Gram – Bacteria, Nuclei:		Red
Additional tissue elements:		Yellow

Note: Also see the Taylor Modification of the Brown and Brenn +/- technique noted for the varying differentiation available. Over-differentiation in the B&B sep 6 is a problem with some sections; run the control slides at varying rates to determine the amount for the specific organism.

References:

• Brown J.H. and Brenn, L. Bull. Johns Hopkins Hosp., 48:69 (1931)
• AFIP Manual of Histologic Staining Techniques: 3rd. ed., ed. G. Luna; New York: McGraw-Hill Publications, c. 1968, p. 222

RT	26105-01	Crystal Violet Staining Solution, 1% Aqueous	500 ml	22.00	Add to Cart
RT	26105-02	Sodium Bicarbonate, 5% Aqueous	500 ml	19.00	Add to Cart
RT	26105-03	Basic Fuchsin Stock Solution, 0.25%, OR	250 ml	24.00	Add to Cart
RT	26105-3A	Basic Fuchsin Working Solution	250 ml	23.00	Add to Cart
RT	26105-04	Gram’s Iodine Solution	500 ml	24.00	Add to Cart
RT	26105-5A	Acetone:Alcohol, 1:1	500 ml	19.00	Add to Cart
RT	26105-06	Picric Acid-Acetone Solution, 0.1%	500 ml	37.00	Add to Cart
RT	26105-07	Acetone	1 qt	15.00	Add to Cart
RT	26105-08	Acetone:Xylene	500 ml	19.00	Add to Cart

Brown-Hopps Method for Gram Positive and Gram Negative Bacteria

For bacterial stain.

Stain results:

Gram Negative Bacteria:		Red
Gram Positive Bacteria:		Blue
Background:		Yellow

References:

• AFIP Manual of Histologic Staining Methods; ed. G. Luna, McGraw-Hill Publications, 3rd. ed.; New York, c. 1968, p.224
• Brown, R.C. and Hopps, H.C., Geographic Pathology Division Armed Forces Institute of Pathology, Washington, D.C. 20305

RT	26106-01	Crystal Violet Staining Solution, 1% Aqueous	500ml	22.00	Add to Cart
RT	26106-02	Gram Iodine Solution	500ml	23.00	Add to Cart
RT	26106-03	Basic Fuchsin Staining Solution, 0.5% Aqueous	500ml	36.00	Add to Cart
RT	26106-04	Gallego’s Differentiating Solution	500ml	22.00	Add to Cart
RT	26106-05	Cellosolve	500ml	33.00	Add to Cart
RT	26106-06	Tartrazine, 1.5% Aqueous	500ml	33.00	Add to Cart

Taylor’s Method for Gram Positive and Gram Negative Bacteria

For bacterial stain.

Stain results:

Gram-Positive Organism:		Blue to Blue-Black
Gram Negative Organism:		Bright Red
Nuclei:		.Brownish Red
Erythrocytes:		Red to Yellow-Green
Necrotic Tissue:		.Yellow-Green
Cytoplasm:		Yellow
Connective Tissue:		Red

References:

• AFIP Manual of Histological Staining Methods, 3rd ed., Ed. L. Luna: New York: McGraw Hill Publications, c. 1968, p.226
• Clark, G.: Staining Procedures, Williams and Wilkins Company, Baltimore, 3rd Ed, c.1973, p.320
• Taylor, R.D., Amer. J. Clin. Path. 46:472 (1966)

RT	26108-01	Harris Hematoxylin	500ml	26.00	Add to Cart
RT	26108-02	Crystal Violet, Hucker’s	500ml	24.00	Add to Cart
RT	26108-03	Basic Fuchsin Stock Sol. 0.1%	500ml	24.00	Add to Cart
RT	26108-04	Gram’s Iodine Solution	500ml	17.00	Add to Cart
RT	26108-05	Lithium Carbonate Solution (Saturated, aqueous)	500ml	18.00	Add to Cart
RT	26108-06	Acid Alcohol, 1%	500ml	17.00	Add to Cart
RT	26108-07	Acetone : Alcohol, 1:1	500ml	14.00	Add to Cart
RT	26108-08	Acetone	500ml	10.00	Add to Cart
RT	26108-09	Picric Acid-Acetone Sol., 0.1%	500ml	22.00	Add to Cart
RT	26108-10	Acetone-Xylene I	500ml	41.00	Add to Cart
RT	26108-11	Acetone-Xylene II	500ml	41.00	Add to Cart

Gridley’s Method for Endamoeba Histolytica

For bacteria stain: Amoebae, their nuclei and ingested erythrocytes

Stain results:

Erythrocytes (ingested):		Rose
Connective tissue:		Green
Amoebae:		Blue-green
Nuclei (amoebic):		Darker blue-green

References:

• Gridley, M.F.: Am. J. Clin. Pathology, 24:243 (1954)
• Luna, L.G.: Histologic Staining Methods, 3rd ed.: New York: McGraw-Hill Book Co., c.1968, p. 228

RT	26109-01	Harris Hematoxylin, OR	500 ml	32.00	Add to Cart
RT	26109-1A	Weigert’s Iron Hematoxylin Solution A, AND	500 ml	30.00	Add to Cart
RT	26109-1B	Weigert’s Iron Hematoxylin Solution B	500 ml	24.00	Add to Cart
RT	26109-02	Aniline-Eosin Solution (Poison Pack)	250 ml	50.00	Add to Cart
RT	26109-03	Naphtol Green B Staining Solution, 1%	250 ml	37.00	Add to Cart
RT	26109-04	Acid Alcohol, 1% in 75% Alcohol Solution	500 ml	27.00	Add to Cart
RT	26109-05	Ammonia Water, 0.3%	500 ml	19.00	Add to Cart

Giemsa for Helicobactor Pylori and Mast Cells

For bacterial, fungal stains.

Stain results:

Red Blood Cells:		Pink
Nuclei:		Blue
H-Pylori, fungi & other bacteria:		Blue
Mast Cells:		Purple
Rickettsia:		Purple

References:

• Helicobactor pylori in peptic ulcer disease, NIH Consensus Statement Online, January 1994, 12(1): 1:23
• Luna 1AFIP 3rd ed. McGraw Hill, 236
• Fail, Rena, AS, HT (ASCP) Medical University of South Carolina, Histologic. Vol. XXXIV, No 2, Nov. 2001, p. 32.

RT	26110-01	Methanol	1 qt	13.50	Add to Cart
RT	26110-02	Giemsa Stock Solution	100ml	23.00	Add to Cart
RT	26110-03	Acetic Acid, 1%	500ml	12.00	Add to Cart

May-Grunwald Giemsa for H-Pylori

For bacterial, fungal stain.

Stain results:

H-pylori:		Blue
Background:		Light purple

References:

• Carson, F. Histotechnology: A Self-Instructional Text. 1980-109.

RT	26114-01	Jenner’s Stain Stock Solution	250 ml	23.00	Add to Cart
RT	26114-02	Giemsa Stock Solution	100ml	24.00	Add to Cart
RT	26114-03	Acetic Acid, 0.1% Solution	500 ml	24.00	Add to Cart

Genta, Robason and Graham Stain for H-pylori & Gastric Morphology

Stain results:

Muscle cells:		Brownish yellow
Lamina propria ground substance:		Light yellowish or grayish
Smooth muscle fibers:		Light Pink
Cystoplasm of neutrophils:		Pale Pink
Eosinophils:		Intense red
Nucleus:		.Dark brown
Intestinal metaplasma:		Bright blue

References:

• Genta PM., Robason GO, Graham DY-Simultaneous Visualization of Helicobactor Pylon and Gastric Morphology A New Stain Hum Pathol. 25:22 1-226, 1994.

RT	26116-01	Uranium Nitrate, 1%	500 ml	48.00	Add to Cart
RT	26116-02	Silver Nitrate, 1% Aqueous	250 ml	50.00	Add to Cart
0-4°C	26116-03	Gum Mastic, 2.5%	250 ml	52.00	Add to Cart
0-4°C	26116-04	Hydroquinone, 2%	250 ml	37.00	Add to Cart
RT	26116-05	Silver Nitrate, 0.04%	250 ml	23.00	Add to Cart
RT	26116-06	Alcian Blue, pH 2.5	250 ml	47.00	Add to Cart
RT	26116-07	Harris Hematoxylin	500 ml	30.00	Add to Cart
RT	26116-08	Acid Alcohol, 1%	500 ml	26.00	Add to Cart
RT	26116-09	Ammonia Water, 0.25%	500 ml	13.00	Add to Cart
RT	26116-10	Eosin Y, 1% Aqueous	500 ml	33.00	Add to Cart

Modified Steiner – Using Chapman’s Modification

For bacterial stain.

Stain results:

Spirochetes, Donovan Bodies, General

Bacteria,Legionairies Disease Bacteria:		Dark Brown or Black
Background:		Bright Yellow to light brown

References:

• Margeson L.S. Chapman CM: Use of Zinc Formalin as a sensitizer in Silver Stain for pirochetes. Journal of Histology 19:2, p 135- 138, 6 –96 (1996)

RT	26119-01	Zinc Formalin	500ml	25.00	Add to Cart
RT	26119-02	Silver Nitrate, 1% Aqueous	250ml	43.00	Add to Cart
0-4°C	26119-03	Gum Mastic, 2.5%	500ml	57.00	Add to Cart
0-4°C	26119-04	Hydroquinone, 2%	500ml	35.00	Add to Cart
RT	26119-05	Silver Nitrate, 0.04%, Aqueous	250ml	29.00	Add to Cart

A.2 FUNGAL STAINS

Gridley’s Method for Fungi

For stain fungal mycelia, conidia, morphological detail of yeast forms and hyphae.

Stain results:

Mycelia, Conidia:		Deep Purple
Elastic Tissue & Mucin Background:		Yellow

References:

• Gridley, M.F.: American J. Clin. Path., 23:303 (1953)
• AFIP Manual of Histologic Staining Techniques: 3rd., Ed. ED. L. Luna, New York: McGraw-Hill Publications, c. 1968, p. 229

RT	26120-01	Chromic Acid, 4%, Aqueous	500 ml	23.00	Add to Cart
0-4°C	26120-02	Coleman’s Feulgen Reagent	250 ml	38.00	Add to Cart
RT	26120-03	Aldehyde Fuchsin Solution	250 ml	29.00	Add to Cart
RT	26120-04	Metanil Yellow, 0.25%	500 ml	36.00	Add to Cart

A Combined Hematoxylin and Eosin/Methenamine Silver Stain for the Histological Diagnosis of Fungi in Tissue Sections

For bacterial, fungal stains.

Stain results:

Organism:		Blue-Black
Background:		Rose

References:

• Lamps, Laura W. et al A Combined Hematoxylin and Eosin/Methenamin Silver Stain for theHistological Diagnosis of Fungi In Tissue Sections., J. of Histotechnology 23:4 p.341 – 345, 2000.

RT	26123-01	Chromic Acid, 10%	500ml	22.00	Add to Cart
RT	26123-02	Sodium Bisulfite, 1%	500ml	15.00	Add to Cart
RT	26123-03	Silver Nitrate, 5% Aqueous	250ml	82.00	Add to Cart
RT	26123-04	Methenamine, 3% Aqueous	500ml	22.00	Add to Cart
RT	26123-05	Sodium Borate, 5% Aqueous	500ml	22.00	Add to Cart
RT	26123-06	Gold Chloride, 0.2%	250ml	56.00	Add to Cart
RT	26123-07	Sodium Thiosulfate, 0.02% Aqu.	500ml	15.00	Add to Cart
RT	26123-08	Harris’ Hematoxylin	500ml	27.00	Add to Cart
RT	26123-09	Acetic Acid 3%	500ml	14.00	Add to Cart
RT	26123-10	Ammonia Water, 0.25%	500ml	16.00	Add to Cart
RT	26123-11	Eosin Y, 1% Alcoholic	500ml	21.00	Add to Cart

A.3 INCLUSION BODY STAINS

Pinkerton’s Method for Rickettsia

For inclusion body stain.

Stain results:

Rickettsia:		Bright Red
Nuclei:		Blue

References:

• Simmons, J.S. and Gentzkow, C.J., Laboratory Methods of the United States Army 5th Ed., Lee & Febiger, Philadelphia, p. 572, 1944
• Luna, L.G., (ed) Manual of Histologic Staining Methods or the AFIP, 3rd Ed., McGraw-Hill, N.Y., . 237, c 1968

RT	26131-01	Methylene Blue Solution, 1% Aqueous Sol	500 ml	42.00	Add to Cart
RT	26131-02	Basic Fuchsin Solution, 0.25%, Aqueous Sol	500 ml	23.00	Add to Cart
RT	26131-03	Citric Acid, 0.5% Aqueous Sol	500 ml	13.00	Add to Cart
RT	26131-04	Gram's Iodine Solution OR	500 ml	23.00	Add to Cart
RT	26131-4A	Lugol’s Iodine	500 ml	26.00	Add to Cart
RT	26131-05	Sodium Thiosulfate, 5% Aqueous Sol	500 ml	16.00	Add to Cart

Wolbach’s Giemsa Method

Wolbach, Todd, and Palfrey (1922)

Nuclei, collagen.

Stain results:

Nuclei, Bacteria:		Blue
Rickettsia:		Purple
Collagen, other tissue elements:		Pink to rose

References:

• Wolbach, S.B., Todd, J.L. and Palfrey, F.W., The Etilogy of Pathology Typhus, Harvard University Press, Cambridge, MA., p. 13 – 14,c 1922
• Luna, L.G., (ed), Manual of Histologic Staining Methods of the AFIP, 3rd Edition, McGraw-Hill, Y, p. 119, c. 1968

RT	26132-01	Giemsa Stock Solution 250 ml OR	250 ml	35.00	Add to Cart
RT	26132-1A	Giemsa Working Solution	500 ml	33.00	Add to Cart
RT	26132-02	Rosin Alcohol Stock Solution, 10% OR	500 ml	32.00	Add to Cart
RT	26132-2A	Rosin Alcohol Working Solution	500 ml	26.00	Add to Cart
RT	26132-03	Lugol’s Iodine OR	500 ml	27.00	Add to Cart
RT	26132-3A	Gram’s Iodine	500 ml	23.00	Add to Cart
RT	26132-04	Sodium Thiosulfate, 5% Aqueous	500 ml	16.00	Add to Cart