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Electron Microscopy Sciences

Histology and Light Microscopy

arrow13Prepared Staining Kits for Histology and Pathology

A.1 BACTERIAL STAINS

Alcoholic Stain SolutionsarrowTruant's Fluorescent Method for Acid Fast Organism

For bacterial stain.

Stain results:

Acid Fast:   Fluoresce reddish yellow
Artifacts:   Fluoresce yellow

References:

  • Truant, J.P. Henry Ford Hospital Med. Bull., 10:287, 1962.
  • AFIP Manual of Histological Staining Methods, 3rd ed. Ed. L. Luna: New York: McGraw Hill Publications. c. 1968. p. 219.
  • Clark, G.: Staining Procedures. Williams and Wilkens Co., Baltimore. 3rd edition. c.1973. p. 225
RT 26102-1A Weigert's Iron Hematoxylin Solution A msds 500 ml 41.00 Add to Cart
RT 26102-1B Weigert's Iron Hematoxylin Solution B msds 500 ml 27.00 Add to Cart
RT 26102-02 Auramine-Rhodamine Staining Solution   250 ml 48.00 Add to Cart
RT 26102-03 Acid Alcohol 1% msds 500 ml 21.00 Add to Cart

Alcoholic Stain SolutionsarrowBrown and Brenn Method for Gram Positive and Gram Negative Bacteria

For bacteria stain: Gram + / Gram – bacteria and filaments of nocardia, etc.

Stain results:

Gram + Bacteria, Nocardia and Actinomyces Filaments:   Blue
Gram – Bacteria, Nuclei:   Red
Additional tissue elements:   Yellow

Note: Also see the Taylor Modification of the Brown and Brenn +/- technique noted for the varying differentiation available. Over-differentiation in the B&B sep 6 is a problem with some sections; run the control slides at varying rates to determine the amount for the specific organism.

References:

  • Brown J.H. and Brenn, L. Bull. Johns Hopkins Hosp., 48:69 (1931)
  • AFIP Manual of Histologic Staining Techniques: 3rd. ed., ed. G. Luna; New York: McGraw-Hill Publications, c. 1968, p. 222
RT 26105-01 Crystal Violet Staining Solution, 1% Aqueous msds 500 ml 24.00 Add to Cart
RT 26105-02 Sodium Bicarbonate, 5% Aqueous msds 500 ml 22.00 Add to Cart
RT 26105-03 Basic Fuchsin, 0.25%, Aqueous msds 250 ml 18.00 Add to Cart
RT 26105-3A Basic Fuchsin Working Solution msds 250 ml 13.00 Add to Cart
RT 26105-04 Gram's Iodine Solution msds 500 ml 22.00 Add to Cart
RT 26105-5A Acetone: Alcohol, 1:1 msds 500 ml 16.00 Add to Cart
RT 26105-06 Picric Acid-Acetone Solution, 0.1% msds 500 ml 33.00 Add to Cart
RT 26105-07 Acetone msds 1 qt 15.00 Add to Cart
RT 26105-08 Acetone: Xylene msds 500 ml 21.00 Add to Cart

Alcoholic Stain SolutionsarrowBrown-Hopps Method for Gram Positive and Gram Negative Bacteria

For bacterial stain.

Stain results:

Gram Negative Bacteria:   Red
Gram Positive Bacteria:   Blue
Background:   Yellow

References:

  • AFIP Manual of Histologic Staining Methods; ed. G. Luna, McGraw-Hill Publications, 3rd. ed.; New York, c. 1968, p.224
  • Brown, R.C. and Hopps, H.C., Geographic Pathology Division Armed Forces Institute of Pathology, Washington, D.C. 20305
RT 26106-01 Crystal Violet Staining Solution, 1% Aqueous msds 500 ml 24.00 Add to Cart
RT 26106-02 Gram Iodine Solution msds 500 ml 22.00 Add to Cart
RT 26106-03 Basic Fuchsin Staining Solution, 0.5% Aqueous msds 500 ml 26.00 Add to Cart
RT 26106-04 Gallego's Differentiating Solution msds 500 ml 19.00 Add to Cart
RT 26106-05 Cellosolve msds 500 ml 31.00 Add to Cart
RT 26106-06 Tartrazine, 1.5% Aqueous msds 500 ml 24.00 Add to Cart

Alcoholic Stain SolutionsarrowTaylor's Method for Gram Positive and Gram Negative Bacteria

For bacterial stain.

Stain results:

Gram-Positive Organism:   Blue to Blue-Black
Gram Negative Organism:   Bright Red
Nuclei:   .Brownish Red
Erythrocytes:   Red to Yellow-Green
Necrotic Tissue:   .Yellow-Green
Cytoplasm:   Yellow
Connective Tissue:   Red

References:

  • AFIP Manual of Histological Staining Methods, 3rd ed., Ed. L. Luna: New York: McGraw Hill Publications, c. 1968, p.226
  • Clark, G.: Staining Procedures, Williams and Wilkins Company, Baltimore, 3rd Ed, c.1973, p.320
  • Taylor, R.D., Amer. J. Clin. Path. 46:472 (1966)
RT 26108-01 Harris Hematoxylin msds 500 ml 31.00 Add to Cart
RT 26108-02 Crystal Violet, Hucker's msds 500 ml 25.00 Add to Cart
RT 26108-03 Basic Fuchsin Stock Sol. 0.1% msds 500 ml 30.00 Add to Cart
RT 26108-04 Gram's Iodine Solution msds 500 ml 20.00 Add to Cart
RT 26108-05 Lithium Carbonate Solution (Saturated, aqueous) msds 500 ml 23.00 Add to Cart
RT 26108-06 Acid Alcohol, 1% msds 500 ml 19.00 Add to Cart
RT 26108-07 Acetone : Alcohol, 1:1 msds 500 ml 16.00 Add to Cart
RT 26108-08 Acetone msds 500 ml 10.00 Add to Cart
RT 26108-09 Picric Acid-Acetone Sol., 0.1% msds 500 ml 31.00 Add to Cart
RT 26108-10 Acetone-Xylene I msds 500 ml 32.00 Add to Cart
RT 26108-11 Acetone-Xylene II msds 500 ml 33.00 Add to Cart

Alcoholic Stain SolutionsarrowGridley's Method for Endamoeba Histolytica

For bacteria stain: Amoebae, their nuclei and ingested erythrocytes

Stain results:

Erythrocytes (ingested):   Rose
Connective tissue:   Green
Amoebae:   Blue-green
Nuclei (amoebic):   Darker blue-green

References:

  • Gridley, M.F.: Am. J. Clin. Pathology, 24:243 (1954)
  • Luna, L.G.: Histologic Staining Methods, 3rd ed.: New York: McGraw-Hill Book Co., c.1968, p. 228
RT 26109-01 Harris Hematoxylin, OR   500 ml 34.00 Add to Cart
RT 26109-1A Weigert's Iron Hematoxylin Solution A, AND msds 500 ml 36.00 Add to Cart
RT 26109-1B Weigert's Iron Hematoxylin Solution B msds 500 ml 26.00 Add to Cart
RT 26109-02 Aniline-Eosin Solution (Poison Pack) msds 250 ml 29.00 Add to Cart
RT 26109-03 Naphtol Green B Staining Solution, 1%   250 ml 13.00 Add to Cart
RT 26109-04 Acid Alcohol, 1% in 75% Alcohol Solution msds 500 ml 18.00 Add to Cart
RT 26109-05 Ammonia Water, 0.3% msds 500 ml 14.00 Add to Cart

Alcoholic Stain SolutionsarrowGiemsa for Helicobactor Pylori and Mast Cells

For bacterial, fungal stains.

Stain results:

Red Blood Cells:   Pink
Nuclei:   Blue
H-Pylori, fungi & other bacteria:   Blue
Mast Cells:   Purple
Rickettsia:   Purple

References:

  • Helicobactor pylori in peptic ulcer disease, NIH Consensus Statement Online, January 1994, 12(1): 1:23
  • Luna 1AFIP 3rd ed. McGraw Hill, 236
  • Fail, Rena, AS, HT (ASCP) Medical University of South Carolina, Histologic. Vol. XXXIV, No 2, Nov. 2001, p. 32.
RT 26110-01 Methanol msds 1 qt 13.50 Add to Cart
RT 26110-02 Giemsa Stock Solution msds 100 ml 14.00 Add to Cart
RT 26110-03 Acetic Acid, 1% msds 500 ml 16.00 Add to Cart

Alcoholic Stain SolutionsarrowMay-Grunwald Giemsa for H-Pylori

For bacterial, fungal stain.

Stain results:

H-pylori:   Blue
Background:   Light purple

References:

  • Carson, F. Histotechnology: A Self-Instructional Text. 1980-109.
RT 26114-01 Jenner's Stain Stock Solution   250 ml 19.00 Add to Cart
RT 26114-02 Giemsa Stock Solution msds 100 ml 14.00 Add to Cart
RT 26114-03 Acetic Acid, 0.1% Solution msds 500 ml 16.00 Add to Cart

Alcoholic Stain Solutions arrowGenta, Robason and Graham Stain for H-pylori & Gastric Morphology

Stain results:

Muscle cells:   Brownish yellow
Lamina propria ground substance:   Light yellowish or grayish
Smooth muscle fibers:   Light Pink
Cystoplasm of neutrophils:   Pale Pink
Eosinophils:   Intense red
Nucleus:   .Dark brown
Intestinal metaplasma:   Bright blue

References:

  • Genta PM., Robason GO, Graham DY-Simultaneous Visualization of Helicobactor Pylon and Gastric Morphology A New Stain Hum Pathol. 25:22 1-226, 1994.
RT 26116-01 Uranium Nitrate, 1% Aqueous msds 500 ml 49.00 Add to Cart
RT 26116-02 Silver Nitrate, 1% Aqueous msds 250 ml 52.00 Add to Cart
0-4°C 26116-03 Gum Mastic, 2.5%   250 ml 52.00 Add to Cart
0-4°C 26116-04 Hydroquinone, 2%   250 ml 37.00 Add to Cart
RT 26116-05 Silver Nitrate, 0.04%   250 ml 23.00 Add to Cart
RT 26116-06 Alcian Blue, pH 2.5 msds 250 ml 47.00 Add to Cart
RT 26116-07 Harris Hematoxylin msds 500 ml 30.00 Add to Cart
RT 26116-08 Acid Alcohol, 1%   500 ml 26.00 Add to Cart
RT 26116-09 Ammonia Water, 0.25%   500 ml 13.00 Add to Cart
RT 26116-10 Eosin Y, 1% Aqueous   500 ml 33.00 Add to Cart

Alcoholic Stain SolutionsarrowModified Steiner – Using Chapman's Modification

For bacterial stain.

Stain results:

Spirochetes, Donovan Bodies, General

Bacteria,Legionairies Disease Bacteria:   Dark Brown or Black
Background:   Bright Yellow to light brown

References:

  • Margeson L.S. Chapman CM: Use of Zinc Formalin as a sensitizer in Silver Stain for pirochetes. Journal of Histology 19:2, p 135-138, 6-96 (1996)
RT 26119-01 Zinc Formalin msds 500 ml 26.00 Add to Cart
RT 26119-02 Silver Nitrate, 1% Aqueous msds 250 ml 46.00 Add to Cart
0-4°C 26119-03 Gum Mastic, 2.5% msds 500 ml 62.00 Add to Cart
0-4°C 26119-04 Hydroquinone, 2% msds 500 ml 40.00 Add to Cart
RT 26119-05 Silver Nitrate, 0.04%, Aqueous msds 250 ml 18.00 Add to Cart

A.2 FUNGAL STAINS

Alcoholic Stain SolutionsarrowGridley's Method for Fungi

For stain fungal mycelia, conidia, morphological detail of yeast forms and hyphae.

Stain results:

Mycelia, Conidia:   Deep Purple
Elastic Tissue & Mucin Background:   Yellow

References:

  • Gridley, M.F.: American J. Clin. Path., 23:303 (1953)
  • AFIP Manual of Histologic Staining Techniques: 3rd., Ed. ED. L. Luna, New York: McGraw-Hill Publications, c. 1968, p. 229
RT 26120-01 Chromic Acid, 4%, Aqueous msds 500 ml 26.00 Add to Cart
0-4°C 26120-02 Coleman's Feulgen Reagent msds 250 ml 25.00 Add to Cart
RT 26120-03 Aldehyde Fuchsin Solution msds 250 ml 26.00 Add to Cart
RT 26120-04 Metanil Yellow, 0.25% msds 500 ml 27.00 Add to Cart

Alcoholic Stain SolutionsarrowA Combined Hematoxylin and Eosin/Methenamine Silver Stain for the Histological Diagnosis of Fungi in Tissue Sections

For bacterial, fungal stains.

Stain results:

Organism:   Blue-Black
Background:   Rose

References:

  • Lamps, Laura W. et al A Combined Hematoxylin and Eosin/Methenamin Silver Stain for theHistological Diagnosis of Fungi In Tissue Sections., J. of Histotechnology 23:4 p.341 – 345, 2000.
RT 26123-01 Chromic Acid, 10% msds 500 ml 37.00 Add to Cart
RT 26123-02 Sodium Bisulfite, 1% msds 500 ml 18.00 Add to Cart
RT 26123-03 Silver Nitrate, 5% Aqueous msds 250 ml 88.00 Add to Cart
RT 26123-04 Methenamine, 3% Aqueous msds 500 ml 24.00 Add to Cart
RT 26123-05 Sodium Borate, 5% Aqueous msds 500 ml 28.00 Add to Cart
RT 26123-06 Gold Chloride, 0.2% Aqueous msds 250 ml 61.00 Add to Cart
RT 26123-07 Sodium Thiosulfate, 0.2% Aqueous msds 500 ml 18.00 Add to Cart
RT 26123-08 Harris' Hematoxylin msds 500 ml 30.00 Add to Cart
RT 26123-09 Acetic Acid 3% msds 500 ml 16.00 Add to Cart
RT 26123-10 Ammonia Water, 0.25% msds 500 ml 17.00 Add to Cart
RT 26123-11 Eosin Y, 1% Alcoholic msds 500 ml 27.00 Add to Cart

A.3 INCLUSION BODY STAINS

Alcoholic Stain SolutionsarrowPinkerton's Method for Rickettsia

For inclusion body stain.

Stain results:

Rickettsia:   Bright Red
Nuclei:   Blue

References:

  • Simmons, J.S. and Gentzkow, C.J., Laboratory Methods of the United States Army 5th Ed., Lee & Febiger, Philadelphia, p. 572, 1944
  • Luna, L.G., (ed) Manual of Histologic Staining Methods or the AFIP, 3rd Ed., McGraw-Hill, N.Y., . 237, c 1968
RT 26131-01 Methylene Blue Solution, 1% Aqueous Solution msds 500 ml 28.00 Add to Cart
RT 26131-02 Basic Fuchsin, 0.25%, Aqueous msds 500 ml 24.00 Add to Cart
RT 26131-03 Citric Acid, 0.5% Aqueous Solution msds 500 ml 14.00 Add to Cart
RT 26131-04 Gram's Iodine Solution OR msds 500 ml 22.00 Add to Cart
RT 26131-4A Lugol's Iodine msds 500 ml 20.00 Add to Cart
RT 26131-05 Sodium Thiosulfate, 5% Aqueous Solution msds 500 ml 19.00 Add to Cart

Alcoholic Stain SolutionsarrowWolbach's Giemsa Method

Wolbach, Todd, and Palfrey (1922)

Nuclei, collagen.

Stain results:

Nuclei, Bacteria:   Blue
Rickettsia:   Purple
Collagen, other tissue elements:   Pink to rose

References:

  • Wolbach, S.B., Todd, J.L. and Palfrey, F.W., The Etilogy of Pathology Typhus, Harvard University Press, Cambridge, MA., p. 13 – 14,c 1922
  • Luna, L.G., (ed), Manual of Histologic Staining Methods of the AFIP, 3rd Edition, McGraw-Hill, Y, p. 119, c. 1968
RT 26132-01 Giemsa Stock Solution 250 ml OR   250 ml 32.00 Add to Cart
RT 26132-1A Giemsa Working Solution msds 500 ml 32.00 Add to Cart
RT 26132-02 Rosin Alcohol Stock Solution, 10% OR   500 ml 31.00 Add to Cart
RT 26132-2A Rosin Alcohol Working Solution   500 ml 27.00 Add to Cart
RT 26132-03 Lugol's Iodine OR msds 500 ml 21.00 Add to Cart
RT 26132-3A Gram's Iodine   500 ml 22.00 Add to Cart
RT 26132-04 Sodium Thiosulfate, 5% Aqueous   500 ml 19.00 Add to Cart

Stain for Blood and Hematological Elements arrow13arrow13