French Flag   chineseFlag   japaneseFlag   germanFlag   SpanishFlag   argentinaFlag   mexicoFlag   russianFlag

View Cart || Request a 2014 Catalog

Electron Microscopy Sciences

Histology and Light Microscopy

arrow14Prepared Staining Kits for Histology and Pathology

B. STAIN FOR BLOOD AND HEMATOLOGIC ELEMENTS

msdsmsdsAlcoholic Stain SolutionsarrowWright-Giemsa Stain

Lillie (1972)

For differentiation of blood corpuscles.

Stain results:

Erthrocytes:   Pink-Tan
Eosinophiles -Granules:   Red
Lymphocytes -Granules:   Red-Purple
-Cytoplasm:   Blue
Neutrophils -Granules:   Purple-Pink

References:

  • Lillie, R.D.: Factors influencing the staining of blood films and the role of methylene violet. J. Lab. Clin. Med. 29:1181, 1944.
  • Lillie, R.D., H.J. Conn’s Biological Stains, 8th Ed, William’s & Wilkins, Baltimore, p. 438, 1972.
RT 26149-01 Wright-Giemsa Stain 500 ml 50.00 Add to Cart
RT 26149-02 Phosphate Buffer, pH 6.5 500 ml 19.00 Add to Cart

Alcoholic Stain SolutionsarrowGiemsa Thin Film Stain

Lillie (1965); Giemsa (1904)

Differentiation of leucocytes, rickettsia, bacteria and inclusion bodies.

Stain results:

Nuclei, additional leukocyte features are similar to

Wright Stain results:   Reddish Purple
Plasmodic cytoplasm:   Blue
Chromatin:   Red

References:

  • Clark, G., Staining Procedures, Williams & Wilkins Co., Baltimore, 3rd Ed., p. 125, c. 1973
RT 26153-01 Giemsa Thin Film Staining Solution, OR 250 ml 24.00 Add to Cart
RT 26153-02 Phosphate Buffer, pH 6.5 500 ml 21.00 Add to Cart
RT 26153-03 Giemsa Stock Solution 250 ml 32.00 Add to Cart

Alcoholic Stain SolutionsarrowGiemsa Thick Film Stain

Lillie (1965); Barber and Komp (1929)

Stain malarial parasites.

Stain Results:

Malarial Parasites:   Clear Red Chromatin
Cytoplasm:   Clear Blue
Red Corpuscles:   Not seen due to lacking of
hemoglobin during dying

References:

  • Clark, G. Staining Procedures, Williams and Wilkins Company, Baltimore, 3rd Ed., p. 126, c. 1973
  • Lillie, R.D. Histologic Technic and Practical Histochemistry, 3rd Ed., McGraw-Hill, New York, 1965.
  • Barber, M.A. & Komp, H.W., Pub. Health Rep., 44:2330-41, c. 1929
RT 26154-01 Giemsa Thick Film Staining Solution,
OR
250 ml 16.00 Add to Cart
RT 26154-02 Phosphate Buffer – pH 7.0 500 ml 20.00 Add to Cart
RT 26154-03 Giemsa Stock Solution 250 ml 31.00 Add to Cart

Alcoholic Stain Solutions arrowThomas Method for Malarial Parasites

Thomas (1953)

Stain Results:

Nuclei:   Blue
Plasma Cell Cytoplasm:   Blue
Malarial Parasites:   Blue
Erythrocytes:   Pink
Other Tissue Elements:   Shades of Rose to Red

References:

  • Thomas, J.T. Stain Techn., 28:311-312, 1953.
  • Luna, L.G. (ed.) Manual of Histologic Staining Methods of the AFIP, 3rd Ed. McGraw-Hill, New York, P.129, c.1968
RT 26155-01 Phloxine B Solution, 0.5% 500 ml 38.00 Add to Cart
RT 26155-02 Methylene Blue – Azur B Staining Solution 500 ml 31.00 Add to Cart
RT 26155-03 Acetic Acid, 0.2% Aqueous 500 ml 17.00 Add to Cart

Alcoholic Stain SolutionsarrowPrice’s Giemsa Stain

Stains for hematologic elements.

Stain results:

Malarial Parasites:   Blue
Tissue Nuclei:   Blue
Bacteria:   Dark Blue
Malarial Pigment:   Black
Schistosonic egg shells:   Blue
Collagen, etc.:   Pale Pink
Erthrocytes:   Pink Rose

References:

  • AFIP Manual of Histological Staining Methods, 3rd ed., L. Luna: New York: McGraw Hill Publications, c. 1968, p. 127
  • Price, D.L., Mil. Med., 133: 363-367, 1968.
RT 26156-01 Giemsa Stock Solution 250 ml 32.00 Add to Cart
RT 26156-02 Phosphate Buffer, pH 7.0 500 ml 21.00 Add to Cart
RT 26156-03 Acetic Acid, 0.2%, Aqueous 500 ml 18.00 Add to Cart
RT 26156-04 Alcoholic Iodine, 2% 500 ml 46.00 Add to Cart

Alcoholic Stain SolutionsarrowPrussian Blue Stain for Non-Hemoglobin Iron

Sundberg and Broman (1955)

Stain results:

Particulate Iron and Non-granular cytoplasm of many

Macrophages:   Blue to Blue-green
Other:   Comparable to original films stained
with Wright Staining Solution

References:

  • Clark, G.: Staining Procedures, Williams & Wilkins Co. Baltimore, 3rd Ed., c.1973, p.130. Sunberg,. R.D. and Broman, H., Blood, 10160-6, 1955.
RT 26161-1A Potassium, Ferrocyanide, 2%, Aqueous 500 ml 22.00 Add to Cart
RT 26161-1B Hydrochloric Acid, 1% 500 ml 24.00 Add to Cart
RT 26161-02 Wright Staining Solution 500 ml 32.00 Add to Cart
RT 26161-03 Phosphate Buffer – pH 6.5 500 ml 21.00 Add to Cart

Alcoholic Stain SolutionsarrowRhodinile Blue Stain

Stains for hematologic elements.

Stain results:

Heinz Bodies:   Deep Purple
Erythrocytes:   Yellow-Orange to Blue-Green
Reticulocytes:   Blue

References:

  • Simpson, C.F., Carlisle, J.W., and Mallard, L.: Stain Technology, 45:22 (1970)
  • Clark, G.: Staining Procedures, Williams and Wilkins Company, Baltimore, 3rd Ed., C. 1973, p. 131.
RT 26162-01 Rhodinile Blue Stain Sol. 500 ml 70.00 Add to Cart

Alcoholic Stain SolutionsarrowPrussian Blue Method for Hemosiderin

Lillie (1965); Highman (1962); Perls (1867)

Stain Results:

Hemosiderin:   Blue or Green
Nuclei:   Red
Background:   Pink

References:

  • Perls, M., Virchow’s Arch. F. Path. Anat., 39:42-48, 1867
  • Highman, B., Arch. Path., 33:937-938, 1942
  • Lillie, R.D., Histopathologic Technic & Practical Histochemistry, 3rd Ed., McGraw Hill, N.Y., 1965
  • Clark, G., (ed.), Staining Procedures, 3rd Ed., Williams & Wilkins, Baltimore, p. 157, c. 1973.
RT 26170-01 Potassium Ferrocyanide, 2% Aqueous   500 ml 22.00 Add to Cart
RT 26170-02 Hydrochloric Acid, 2%   500 ml 21.00 Add to Cart
RT 26170-03 Safranin O, 0.2% msds 500 ml 19.00 Add to Cart
RT 26170-04 Acetic Acid, 1% Aqueous msds 500 ml 27.00 Add to Cart

Alcoholic Stain Solutionsarrow3-Amino-9-Ethyl Carbazole Method for Leukocyte Peroxidase

Stains for hematologic elements.

Stain results:

Sites of Peroxidase activity:   Red-Brown Granules
Nuclei:   Blue

References:

  • Williams, W.j., Beutler, E., Erslev, A.J. and Rundles, R.W. Hematology, 2nd Ed. McGraw-Hill, YY, c 1977, pp 1627-1628.
RT 26173-01 Formalin-Acetone Fixative   500 ml 17.00 Add to Cart
RT 26173-02 3-Amino-9-Ethylcarbazole
Staining Solution
  500 ml 39.00 Add to Cart
RT 26173-03 Mayer Hematoxylin msds 500 ml 37.00 Add to Cart

Bone, Calcium, and Bone Marrow Stains arrow12arrow12