B. STAIN FOR BLOOD AND HEMATOLOGIC ELEMENTS

Wright-Giemsa Stain

Lillie (1972)

For differentiation of blood corpuscles.

Stain results:

Erthrocytes:		Pink-Tan
Eosinophiles -Granules:		Red
Lymphocytes -Granules:		Red-Purple
-Cytoplasm:		Blue
Neutrophils -Granules:		Purple-Pink

References:

• Lillie, R.D.: Factors influencing the staining of blood films and the role of methylene violet. J. Lab. Clin. Med. 29:1181, 1944.
• Lillie, R.D., H.J. Conn’s Biological Stains, 8th Ed, William’s & Wilkins, Baltimore, p. 438, 1972.

RT	26149-01	Wright-Giemsa Stain	500 ml	52.00	Add to Cart
RT	26149-02	Phosphate Buffer, pH 6.5	500 ml	17.00	Add to Cart

Giemsa Thin Film Stain

Lillie (1965); Giemsa (1904)

Differentiation of leucocytes, rickettsia, bacteria and inclusion bodies.

Stain results:

Nuclei, additional leukocyte features are similar to

Wright Stain results:		Reddish Purple
Plasmodic cytoplasm:		Blue
Chromatin:		Red

References:

• Clark, G., Staining Procedures, Williams & Wilkins Co., Baltimore, 3rd Ed., p. 125, c. 1973

RT	26153-01	Giemsa Thin Film Staining Solution, OR	250 ml	30.00	Add to Cart
RT	26153-02	Phosphate Buffer, pH 6.5	500 ml	17.00	Add to Cart
RT	26153-03	Giemsa Stock Solution	250 ml	35.00	Add to Cart

Giemsa Thick Film Stain

Lillie (1965); Barber and Komp (1929)

Stain malarial parasites.

Stain Results:

Malarial Parasites:		Clear Red Chromatin
Cytoplasm:		Clear Blue
Red Corpuscles:		Not seen due to lacking of hemoglobin during dying

References:

• Clark, G. Staining Procedures, Williams and Wilkins Company, Baltimore, 3rd Ed., p. 126, c. 1973
• Lillie, R.D. Histologic Technic and Practical Histochemistry, 3rd Ed., McGraw-Hill, New York, 1965.
• Barber, M.A. & Komp, H.W., Pub. Health Rep., 44:2330-41, c. 1929

RT	26154-01	Giemsa Thick Film Staining Solution, OR	250 ml	28.00	Add to Cart
RT	26154-02	Phosphate Buffer – pH 7.0	500 ml	17.00	Add to Cart
RT	26154-03	Giemsa Stock Solution	250ml	35.00	Add to Cart

Thomas Method for Malarial Parasites

Thomas (1953)

Stain Results:

Nuclei:		Blue
Plasma Cell Cytoplasm:		Blue
Malarial Parasites:		Blue
Erythrocytes:		Pink
Other Tissue Elements:		Shades of Rose to Red

References:

• Thomas, J.T. Stain Techn., 28:311-312, 1953.
• Luna, L.G. (ed.) Manual of Histologic Staining Methods of the AFIP, 3rd Ed. McGraw-Hill, New York, P.129, c.1968

RT	26155-01	Phloxine B Solution, 0.5%	500 ml	43.00	Add to Cart
RT	26155-02	Methylene Blue – Azur B Staining Solution	500 ml	58.00	Add to Cart
RT	26155-03	Acetic Acid, 0.2% Aqueous	500 ml	20.00	Add to Cart

Price’s Giemsa Stain

Stains for hematologic elements.

Stain results:

Malarial Parasites:		Blue
Tissue Nuclei:		Blue
Bacteria:		Dark Blue
Malarial Pigment:		Black
Schistosonic egg shells:		Blue
Collagen, etc.:		Pale Pink
Erthrocytes:		Pink Rose

References:

• AFIP Manual of Histological Staining Methods, 3rd ed., L. Luna: New York: McGraw Hill Publications, c. 1968, p. 127
• Price, D.L., Mil. Med., 133: 363-367, 1968.

RT	26156-01	Giemsa Stock Solution	250ml	39.00	Add to Cart
RT	26156-02	Phosphate Buffer, pH 7.0	500ml	20.00	Add to Cart
RT	26156-03	Acetic Acid, 0.2%, Aqueous	500ml	17.00	Add to Cart
RT	26156-04	Alcoholic Iodine, 2%	500ml	52.00	Add to Cart

Prussian Blue Stain for Non-Hemoglobin Iron

Sundberg and Broman (1955)

Stain results:

Particulate Iron and Non-granular cytoplasm of many

Macrophages:		Blue to Blue-green
Other:		Comparable to original films stained with Wright Staining Solution

References:

• Clark, G.: Staining Procedures, Williams & Wilkins Co. Baltimore, 3rd Ed., c.1973, p.130. Sunberg,. R.D. and Broman, H., Blood, 10160-6, 1955.

RT	26161-1A	Potassium, Ferrocyanide, 2%, Aqueous	500 ml	26.00	Add to Cart
RT	26161-1B	Hydrochloric Acid, 1%	500 ml	21.00	Add to Cart
RT	26161-02	Wright Staining Solution	500 ml	30.00	Add to Cart
RT	26161-03	Phosphate Buffer – pH 6.5	500 ml	17.00	Add to Cart

Rhodinile Blue Stain

Stains for hematologic elements.

Stain results:

Heinz Bodies:		Deep Purple
Erythrocytes:		Yellow-Orange to Blue-Green
Reticulocytes:		Blue

References:

• Simpson, C.F., Carlisle, J.W., and Mallard, L.: Stain Technology, 45:22 (1970)
• Clark, G.: Staining Procedures, Williams and Wilkins Company, Baltimore, 3rd Ed., C. 1973, p. 131.

RT

26162-01

Rhodinile Blue Stain Sol.

500ml

83.00

Add to Cart

Prussian Blue Method for Hemosiderin

Lillie (1965); Highman (1962); Perls (1867)

Stain Results:

Hemosiderin:		Blue or Green
Nuclei:		Red
Background:		Pink

References:

• Perls, M., Virchow’s Arch. F. Path. Anat., 39:42-48, 1867
• Highman, B., Arch. Path., 33:937-938, 1942
• Lillie, R.D., Histopathologic Technic & Practical Histochemistry, 3rd Ed., McGraw Hill, N.Y., 1965
• Clark, G., (ed.), Staining Procedures, 3rd Ed., Williams & Wilkins, Baltimore, p. 157, c. 1973.

RT	26170-01	Potassium Ferrocyanide, 2% Aqueous	500 ml	25.00	Add to Cart
RT	26170-02	Hydrochloric Acid, 2%	500 ml	23.00	Add to Cart
RT	26170-03	Safranin O, 0.2%	500 ml	33.00	Add to Cart
RT	26170-04	Acetic Acid, 1% Aqueous	500 ml	20.00	Add to Cart

3-Amino-9-Ethyl Carbazole Method for Leukocyte Peroxidase

Stains for hematologic elements.

Stain results:

Sites of Peroxidase activity:		Red-Brown Granules
Nuclei:		Blue

References:

• Williams, W.j., Beutler, E., Erslev, A.J. and Rundles, R.W. Hematology, 2nd Ed. McGraw-Hill, YY, c 1977, pp 1627-1628.

RT	26173-01	Formalin-Acetone Fixative	500ml	52.00	Add to Cart
RT	26173-02	3-Amino-9-Ethylcarbazole Staining Solution	500ml	38.00	Add to Cart
RT	26173-03	Mayer Hematoxylin	500ml	26.00	Add to Cart

Bone, Calcium, and Bone Marrow Stains