C. BONES AND CALCIUM STAINS

Canaliculi and Lacunae Stain

Powers, Rasmussen and Clark (1951)

For bones and teeth.

Stain results:

Canaliculi, lacunae, odontoblast and dentinal tubules:

Bluish to purplish black

References:

• Powers, M.M., Rasmusen, G.L., and Clark, G.,Anat. Rec., 111:117- 6, (1951)
• Clark, G. (ed), Staining Procedures, 3rd edition, William & Wilkins, Baltimore, c 1973, p. 136
• Bodian, D., Anat. Rec., 65:89 – 97, 1936
• Bodian, D., Anat. Rec., 69:153 – 62, 1937

RT	26201-01	Cupric Nitrate, 1% Aqueous	500 ml	27.00	Add to Cart
RT	26201-02	Protargol Solution, 1% Aqueous	500 ml	59.00	Add to Cart
RT	26201-03	Copper Shot	50 g	27.00	Add to Cart
0-4°C	26201-04	Hydroquinone Reducing Solution	500 ml	26.00	Add to Cart
RT	26201-05	Gold Chloride, 1% Aqueous	250 ml	122.00	Add to Cart
RT	26201-06	Oxalic Acid, 2% Aqueous	500 ml	22.00	Add to Cart
RT	26201-07	Sodium Thiosulfate, 5% Aqueous	500 ml	16.00	Add to Cart

Modified Alizarin Red S for Fetal Specimens

Cumley, Crow, and Griffin (1939)

For Minute bones and fetal ossification in mammalian embryos

Stain results:

Bone:		Red
Soft Tissue: .		Transparent and unstained

References:

• Cumley, R.W., Crow, J.F., and Griffin, A.B., Stain Techn., 14:7 – 11, 1939
• Dawson, A.B., Stain Techn., 1:123 – 124, 1926
• Richmond, G.W. and Bennett, L., Stain Techn., 13:77 – 79, 1938
• Clark, G., (ed), Staining Procedures, 3rd ed., William & Wilkins, Baltimore, p. 138, c. 1973

RT	26204-01	Alizarin S, 0.01% Aqueous	500 ml	42.00	Add to Cart
RT	26204-02	Potassium Hydroxide, 1% Aqueous	500 ml	32.00	Add to Cart
RT	26204-03	Potassium Hydroxide, 10% Aqueous	500 ml	30.00	Add to Cart
RT	26204-04	Glycerol-Potassium Hydroxide	500 ml	37.00	Add to Cart

Alizarin Red S and Toluidine Blue O

Williams (1941); Dawson (1926)

Distinction between bone and cartilage in mammalian embryos.

Stain results:

Soft Tissues:		Transparent
Osseous Tissues:		Deep Red
Cartilage:		Dark Blue

References:

• Dawson A.B., Stain Techn., 1:123-4, 1926
• Williams, T.W., Stain Techn., 16:23-5, 1941
• Clark, G., (ed), Staining Procedures, 3rd Ed., Williams & Wilkins Baltimore, p. 139, c. 1973

RT	26205-01	Alizarin Red S, 0.02%, Aqueous	500 ml	43.00	Add to Cart
RT	26205-1A	Potassium Hydroxide, 4%, Aqueous	500 ml	26.00	Add to Cart
RT	26205-02	Toluidine Blue O, 0.25%	500 ml	33.00	Add to Cart
RT	26205-03	Ammonium Alcohol	500 ml	27.00	Add to Cart
RT	26205-05	Potassium Hydroxide, 2%, Aqueous	500 ml	26.00	Add to Cart
RT	26205-06	Sulfuric Acid Alcohol	500 ml	29.00	Add to Cart

Alizarin Red S for Calcium Deposits

Calcium deposits stain.

Stain Results:

Calcium Sites:		Covered and surrounded by a heavy orange red precipitate which is birefringent between crossed polaroids
Background:		Faint Pink

References:

• McGee-Russell, S.M., J. Histochem., 6:22-42, 1958.
• Clark, G., (ed), Staining Procedures, 3rd d., Williams & Wilkins, Baltimore, p. 140, c. 1973.

RT	26206-01	Alizarin S, 2% Solution, pH 4.2	250 ml	50.00	Add to Cart
RT	26206-02	Reagent Alcohol, 50%	500 ml	23.00	Add to Cart
RT	26206-03	Acetone	1 qt	14.00	Add to Cart
RT	26206-04	Acetone – Xylene	500 ml	19.00	Add to Cart

Pizzolato’s Method for Calcium Oxalate

For bone and its related stain.

Stain results:

Calcium oxalate:		Black
All other tissue elements:		Red

References:

• Pizzolato, P., J. Histochem. Cytochem., 12:333-136, 1964.
• Luna, L.G., (ed), Manual of Staining Methods of the AFIP, 3rd Ed, McGraw-Hill, NY, c 1968, p 77.

RT	26212-01	Silver Nitrate, 5% Aqueous	250ml	77.00	Add to Cart
RT	26212-02	Hydrogen Peroxide, 30%	450ml	42.00	Add to Cart
RT	26212-03	Nuclear Fast Red (Kennechtrot) Solution	500ml	40.00	Add to Cart

D. BONE MARROW STAINS

May-Grunwald / Giemsa Combined Stain

Beck (1938); Pappanheim (1912)

Stain results:

Nuclei, Bacteria		Blue
Cytoplasm		Pink-Rose

References

• Beck, R.C., Laboratory Manual of Hematological Technique, W.B. Saunders & Co., Philadelphia, c. 1938, pp 233 – 236
• Clark, G. (ed), Staining Procedures, 3rd ed. Williams &Wilkins, Baltimore, c. 1973, pp 131 – 132
• Strumia, M.M., J Lab Clin. Med 21:930 – 934, 1935 – 1936
• Luna, L.G., (ed) Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology, 3rd ed. McGraw-Hill, NY, c. 1968, pp 121-122

RT	26250-01	May-Grunwald Staining Solution OR	500ml	29.00	Add to Cart
RT	26250-1A	Jenner Staining Solution	500ml	39.00	Add to Cart
RT	26250-02	Giemsa Staining Solution	250ml	35.00	Add to Cart

Maximow’s Method for Bone Marrow

Mallory (1961)

Morphology details of marrow cells.

Stain results:

Nuclei		Blue
Basophile leukocyte, mast cell granules		Purple to Violet
Cartilage		Purple
Erythrocytes, eosinophile granules		Pink
Cytoplasm		Blue to Pink

References

• AFIP Manual of Histologic Staining Methods, 3rd Ed, Ed. L. Luna: New York: McGraw Hill Publications, c. 1968, p. 120
• Mallory, F.B., Pathological technique, New York, Hafner Publishing Co., 1961, p.196

RT	26252-01	Mayer’s Hematoxylin	500ml	46.00	Add to Cart
RT	26252-02	Eosin Y, 0.1%, Aqueous	500ml	47.00	Add to Cart
RT	26252-03	Azur II, 0.1%, Aqueous	500 ml	57.00	Add to Cart
RT	26252-04	Phosphate Buffer, 0.1M, pH 6.8 – 7.0	500 ml	17.00	Add to Cart

Carbohydrate and Mucosubstance Stains