E. CARBOHYDRATE & MUCOSUBSTANCE STAINS:

Crystal Violet Stain for Amyloid

Stain results:

Amyloid:		Violet-Red
Tissue:		Blue

References:

• Clark, G.: Staining Procedures, Williams and Wilkin co., Baltimore 3rd Ed., c. 1973, p.48.
• Conn, H. J., Biological Stains 4th Ed. Biotech Publications, Geneva New York, 1940.

RT	26301-01	Crystal Violet Solution	500ml	57.00	Add to Cart
RT	26301-02	Acetic Acid, 1% Aqueous	500ml	14.00	Add to Cart

Sweat-Puchtler Method for Amyloid

Stain results:

Amyloid:		Pink-Red
Nuclei:		Blue
Elasticia:		Pink to Red
Background:		Unstained

References:

• Sweat, F. and Puchtler, H.: Arch. Path. 80:613, 1965
• AFIP Manual of Histologic Staining Methods, 3rd edition, Ed L. Luna: New York: McGraw Hill Publication, c. 1968, p. 154

RT	26303-01	Sirius Red F3BA Solution, 1%	500ml	42.00	Add to Cart
RT	26303-02	Mayer Hematoxylin	500ml	27.00	Add to Cart
RT	26303-03	Borate Buffer, 0.1M – pH 9.0	500ml	33.00	Add to Cart
RT	26303-04	Alkaline Alcohol5	500ml	23.00	Add to Cart

Laqueur’s Method for Alcoholic Hyalin Lacqueur (1950)

For mallory bodies, erythrocytes, bile pigment and proteinaceous
material in liver.

Stainresults:

Mallory Bodies:		Bright Red
Erythrocytes:		Red
Cytoplasm:		Pale Brown
Bile Pigment:		Green
Proteinaceous material seen occasionally in some liver cells:		Red
Hemosiderin and lipofichsin:		Unstained

References:

• Laqueur, G.I., Amer. J. Clin. Path., 20:689-690, 1950
• Luna, L.G. (ed), Manual of Hitopathologic Staining Method of the AFIP, 3rd ed, McGraw-Hill, NY, c. 1968, p 160
• Lillie, R.D., Fullmer, H.M., Histopathologic Technic and Practical Histochemistry 4th ed., cGraw-Hill, NY, 1976, p 670-671
• Clark, G., (ed) Staining Procedures, 3rd ed. WilliamS & Wilkins, Baltimore, p 51, c. 1973

RT	26306-01	Mayer’s Hematoxylin	500 ml	46.00	Add to Cart
RT	26306-02	Acid Fuchsin-Aniline Solution	500 ml	59.00	Add to Cart
RT	26306-03	Light Green, 1% Aqueous	500 ml	37.00	Add to Cart
RT	26306-04	Phosphomolybdic Acid, 1% Aqueous	500 ml	29.00	Add to Cart
RT	26306-05	Alcoholic Picric Acid	500 ml	49.00	Add to Cart

E.2 Glycogen Stains

McManus’ Method (PAS) for Glycogen

McManus (1948)

Stain results:

Nuclei:		Blue
Fungi:		Red
Background, when Light Green is used as the counter-stain:		Green, pale

References:

• McManus, JFA: Stain Tech.; 23:99 (1948)
• AFIP Manual of Histologic Staining Techniques; L.G. Luna, 3rd Ed., New York: McGraw-Hill Publications, c. 1968, p. 160.
• Mowry, RW: Annals of the New York Academy of Science, 106:402 (1963).

RT	26312-01	Coleman’s Feulgen, Refrigerated! OR	500 ml	41.00	Add to Cart
RT	26312-1A	Schiff’’s reagent, Refrigerated!	500 ml	42.00	Add to Cart
RT	26312-02	Light Green Stock Solution, 0.2% l OR	250 m	23.00	Add to Cart
RT	26312-2A	Light Green Working Solution OR	500 ml	30.00	Add to Cart
RT	26312-2B	Harris Hematoxylin	500 ml	30.00	Add to Cart
RT	26312-03	Periodic Acid, 0.5%, Aqueous	500 ml	30.00	Add to Cart
RT	26312-04	Acid – Alcohol, 1%	500 ml	26.00	Add to Cart
RT	26312-05	Ammonia Water, 0.3%	500 ml	14.00	Add to Cart

E.3 Mucosubstance Stains

Mayer Mucicarmine Method

For mucosubstances stains.

Stain results:

Mucin, Cryptococci Capsulae:		Deep Rose to Red
Other Tissue Elements:		Yellow
Nuclei:		Black

Note: The rose color due to carmin staining will be obscured if sections
are overstained with Weigert’s Hematoxylin and/or Metanil Yellow
solution

References:

• Mallory, F.B.: Pathological Technique. Hafner Publishing Co., N.Y, c. 1961, p. 130.
• Luna, L.G.., (ed), AFIP Manual of Histologic Staining Methods, 3rd. ed., McGraw-Hill Co., NY, C. 1968, pp161-162
• Sheehan, D.C. & Hrapenak, B.B., Theory and Practice of Histotechnology, 2nd ed., C.V. Mosby Co., St. Louis. C. 1980. pp 168-169

RT	26320-01	Mucicarmine Stock Solution	500ml	63.00	Add to Cart
RT	26320-02	Metanil Yellow, 0.25%	500ml	24.00	Add to Cart
RT	26320-03	Weigert’s Hematoxylin Solution A	500ml	34.00	Add to Cart
RT	26320-04	Weigert’s Hematoxylin Solution B	500ml	26.00	Add to Cart

Modification of Mayer’s Mucihematein

Lillie (1965); Laskey (1950)

For stain mucins, especially those derived from epithelial cells of
glandular tissue.

Stain results:

Mucin:		Deep Violet
Cell Nuclei:		Pale Gray Blue
Connective Tissue:		Pale Gray to colorless

References:

• Laskey, Alice: Stain Technology, 25:33 (1959)
• Clark G.: Staining Procedures, Williams & Wilkins Co., Baltimore, 3rd Ed., c. 1973, p. 57.

RT

26321-01

Mayer’s Mucihematein, Modified

500 ml

72.00

Add to Cart

Alcian Blue Method (pH 2.5)

For stain weakly acidic sulfated mucosubstances, hyaluronic acid and
sialomucins.

Stain results:

Weakly Acidic Sulfated Mucosubstances:		Dark Blue
Hyaluronic Acid:		Dark blue
Sialomucins:		Dark blue

References:

• AFIP Manual of Histological Stains Methods, 3rd Ed, Ed. L. Luna: New York McGraw Hill Publications, c. 1968, p. 163.
• Lev, R. & Spicer. S.S., J. Histochem Cystochem, 12:309, 1964

RT	26323-01	Alcian Blue, 1% - pH 2.5	250 ml	47.00	Add to Cart
RT	26323-02	Nuclear Fast Red (Kernechtrot) Solution	250 ml	42.00	Add to Cart
RT	26323-03	Acetic Acid, 3% Aqueous	500 ml	23.00	Add to Cart

Aldehyde-Fuchsin Method (pH 1.0)

For stain highly acid sulfated mucosubstances.

Stain results:

All mucins except the highly acidic sulfated mucosubstances are
aldehyde fuchsin negative at pH values of 1.0 and below.

References:

• Johnson, W.C., Graham, J.H., and Helwig, E.B., J. Invest. Derm., 42:215-224, 1964
• Luna, L.G., (ed), Manual of Histologic Staining Methods of the AFIP, 3rd Ed., McGraw-Hill, c.1968, p. 166

RT	26328-01	Aldehyde-Fuchsin Solution – pH 1.0	500 ml	42.00	Add to Cart
RT	26328-02	Metanil Yellow Solution, 0.25%	500 ml	27.00	Add to Cart
RT	26328-03	Alcohol Solution, 80% - pH 1.0	500 ml	23.00	Add to Cart

Connective Tissue Stains