Prepared Staining Kits for Histology and Pathology
F. CONNECTIVE TISSUE STAINS
F.1 Van Gieson Stains and related
Van Gieson’s Method for Collagen Fibers
Mallory (1938); Van Gieson (1889)
For collagen, muscle and cornified epithelium.
Stain results:
| Collagen: | Red | |
| Smooth and striated muscle: | Yellowish to Brownish | |
| Cornified epithelium: | Yellow | |
| Hyalin: | Yellow | |
| Nuclei: | Blue to Black |
References:
- Clark, G., (ed) Staining Procedures, 3rd Ed., Williams & Wilkins Co., Baltimore, c. 1973, p. 50
- Luna, L (ed) AFIP Manual o f Histological Staining Methods, 3rd ED., New York: McGraw Hill Publications, c 1968, p.76
- Van Gieson, I., New York Med . J., 50:57-60, 1889.
- Mallory,F.B., Pathological Technique, Hafner Publishing., New York, c. 1961, p. 152
| RT | 26350-01 | Van Gieson’s Solution | 500 ml | 23.00 | Add to Cart |
| RT | 26350-02 | Weigert’s Iron Hematoxylin A & |
500 ml | 43.00 | Add to Cart |
| RT | 26350-03 | Weigert’s Iron Hematoxylin B | 500 ml | 29.00 | Add to Cart |
Puchtler-Sweat Method for
Basement Membranes
Puchtler and Sweat (1964)
Stain results:
| Basement Membranes: | Black in cross section/ Gray in tangential sections |
|
| Nuclei: | Pink to Red |
References:
- Puchtler, H. and Sweat, F.: Stain Technology, 39:163, 1964
- AFIP Manual of Histological Staining Mehtods, 3rd ed., Ed. L. Luna: NewYork, McGraw Hill Pub., c. 1968, p. 73
| RT | 26356-01 | Resorcin-Fuchsin Solution | 500 ml | 42.00 | Add to Cart |
| RT | 26356-02 | Nuclear Fast Red (Kernechtrot) Solution | 500 ml | 37.00 | Add to Cart |
| RT | 26356-03 | Periodic Acid, 0.5% Aqueous | 500 ml | 29.00 | Add to Cart |
| RT | 26356-04 | Sodium Bisulfite Solution | 500 ml | 31.00 | Add to Cart |
Picrosirius Red Staining of Cardiac
Muscle Following Phosphomolybdic Acid Treatment
For connective tissue stains – Van Gieson stains and variants.
Stain results:
| Septa: | Red |
References:
- Dolber, Paul C. and Madison S. Spach. Picrosirus Red Staining of Cardiac Muscle Following Phosphomolybdic Acid Treatment, Stain Technology, Vol. 62 No 1 (1987) 23 – 26.
| RT | 26357-01 | Phosphomolybdic Acid, 0.2% Aqueous | 500ml | 25.00 | Add to Cart |
| RT | 26357-02 | Sirius Red, 0.1% in SaturatedPicric Acid | 100ml | 33.00 | Add to Cart |
| RT | 26357-03 | Hydrochloric Acid, 0.01N | 500ml | 17.00 | Add to Cart |
F.2 Stains with Poly-Acid Step
Lillie Modification of
Masson’s Trichrome
Lillie (1940)
Stains cells, cytoplasm muscle and collagen of mammalian tissue
Stain results:
| Nuclei: | Black | |
| Cystoplasm: | Brown to Pink | |
| Muscle: | Red | |
| Erythrocytes: | Brilliant Scarlet | |
| Collagen: | Blue to Blue Green | |
| Myelinated fibers: | Red | |
| Fibrin: | Red to Pink |
References:
- Clark, G.: Staining Procedures. Williams & Wilkins Co., Baltimore, 3rd ed., c. 1973, p.59
- Lillie, R.D., Arch. Path., 29:705, 1940
| RT | 26364-01 | Phosphomolybdic/Phosphotungstic Acid Solution | 500 ml | 62.00 | Add to Cart |
| RT | 26364-02 | Weigert’s Iron Hematoxylin A | 500 ml | 43.00 | Add to Cart |
| RT | 26364-03 | Weigert’s Iron Hematoxylin B | 500 ml | 30.00 | Add to Cart |
| RT | 26364-04 | Biebrich Scarlet Solution, 1% | 500 ml | 28.00 | Add to Cart |
| RT | 26364-05 | Fast Green FCF Solution, 2.5% | 500 ml | 30.00 | Add to Cart |
| RT | 26364-06 | Acetic Acid, 1% Aqueous | 500 ml | 22.00 | Add to Cart |
| RT | 26364-07 | Lugol’s Iodine OR |
500 ml | 26.00 | Add to Cart |
| RT | 26364-7A | Gram’s Iodine | 500 ml | 23.00 | Add to Cart |
| RT | 26364-08 | Sodium Thiosulfate, 5% Aqueous | 500 ml | 18.00 | Add to Cart |
Masson’s Trichrome for Connective Tissue
Sheehan and Hrapchak (1980)
For stain nuclei, agentaffen granules, collagen, cytoplasm, keratin, etc.
Stain results:
| Nuclei: | Black | |
| Cytoplasm, Keratin, Muscle Fibers: | Red | |
| Collagen, Mucin: | Blue |
References:
- Sheehan and Hrapchak: Theory and Practice of Histotechnology, St. Louis. The Moxby Company 1980, p. 190
| RT | 26367-01 | Bouin’s Fixative | 500 ml | 22.00 | Add to Cart |
| RT | 26367-02 | Weigert’s Iron Hematoxylin A | 500 ml | 43.00 | Add to Cart |
| RT | 26367-03 | Weigert’s Iron Hematoxylin B | 500 ml | 29.00 | Add to Cart |
| RT | 26367-04 | Biebrich Scarlet Solution, 1% | 500 ml | 29.00 | Add to Cart |
| RT | 26367-05 | Phosphomolybdic/Phosphotungstic Acid Solution | 500 ml | 73.00 | Add to Cart |
| RT | 26367-06 | Aniline Blue Solution | 500 ml | 46.00 | Add to Cart |
| RT | 26367-07 | Acetic Acid, 1% Aqueous | 500 ml | 22.00 | Add to Cart |
F.3 Elastic Tissue Stains
Modified Verhoff Elastic Van Gieson Stain
For connective tissue stains – Stain with a poly-acid step
Stain results:
| Nuclei: | Grayish-Black | |
| Collagen Fibers: | Red | |
| Elastic Fibers: | Bluish-Black | |
| Muscle: | Greenish-Yellow |
References:
- Gravey, Winsome et al “A modified Verhoff Elastic Van Gieson Stain” The Journal of Histotechnology, Vol. 14, No. 2, pp113 –114. June 1991.
| RT | 26369-01 | Alcoholic Hematoxylin, 3% | 250ml | 36.00 | Add to Cart |
| RT | 26369-02 | Ferric Chloride, 2% aqueous | 500ml | 15.00 | Add to Cart |
| RT | 26369-03 | Lugol’s Iodine | 500ml | 19.00 | Add to Cart |
| RT | 26369-04 | Ferric Chloride, 0.4% aqueous | 500ml | 14.00 | Add to Cart |
| RT | 26369-05 | Van Geisons Solution | 250m | 29.00 | Add to Cart |
Weigert’s Resorcin Fuchsin
Lillie (1965); Mallory (1938); Weigert (1898)
For stain elastic fibers in blood vessel walls, nuclei and collagen.
Stain results:
| Elastic Tissues: | Dark Blue | |
| Nuclei (red if Orth’s Carmine used): | Dark Blue | |
| Collagen: | Red-Pink | |
| Miscellaneous Tissue Elements: | Yellow (with Van Gieson’s stain) |
References:
- Mallory, FB: Pathological Technique; New York: Hafner Publishing Co., c. 1961, p. 168
- Clark G: Staining Procedures, Baltimore: Williams & Wilkins Co., 3rd ed. c. 1973, p.60
- Luna, I. G.: AFIP Histologic Staining Methods: New York: McGraw Hill Book Co., 3rd ed., c. 1968, p. 80
| RT | 26370-01 | Resorcin-Fuchsin Solution | 500 ml | 36.00 | Add to Cart |
| RT | 26370-02 | Van Gieson’s Solution | 500 ml | 23.00 | Add to Cart |
| RT | 26370-03 | Weigert’s Iron Hematoxylin A | 500 ml | 43.00 | Add to Cart |
| RT | 26370-04 | Weigert’s Iron Hematoxylin B | 500 ml | 29.00 | Add to Cart |
| RT | 26370-05 | Acid Alcohol, 1% | 500 ml | 19.00 | Add to Cart |
Verhoeff’s Van-Gieson’s (VVG Method)
Sheehan and Hrapchak (1980)
For stain elastic fibers, nuclei, collagen and other tissue elements.
Stain results:
| Elastic Fibers: | Blue-black to Black–Fine elastic fibrils may not be stained with this method |
|
| Nuclei: | Blue to Black | |
| Collagen: | Red | |
| Other Tissue Elements: | Yellow |
References:
- Sheehan, Dezna C., Hrapchack, Barbara B.: Theory and Practice of Histotechnology, 2nd ed. The C.V. Mosby C., St Louis, p. 196-197, c. 1980
| RT | 26374-01 | Alcoholic Hematoxylin, 5% | 500 ml | 53.00 | Add to Cart |
| RT | 26374-02 | Ferric Chloride, 10% | 500 ml | 29.00 | Add to Cart |
| RT | 26374-03 | Weigert’s Iodine | 500 ml | 26.00 | Add to Cart |
| RT | 26374-04 | Ferric Chloride, 2% | 500 ml | 19.00 | Add to Cart |
| RT | 26374-05 | Sodium Thiosulfate, 5% Aqueous | 500 ml | 18.00 | Add to Cart |
| RT | 26374-06 | Van-Gieson’s Solution | 500 ml | 23.00 | Add to Cart |
Carstairs Method for Fibrin and Platelets
Carstairs (1965)
For the staining of fibrin and platelets.
Stain results:
48 hours or more |
Less than 48 hours | |
| Fibrin: | Bright Red |
Orange to Orange-Red |
| Platelets | Gray blue to navy |
Light Gray |
| Collagen: | Bright Blue |
Bright Blue |
| Muscle: | Red |
Red |
| Red Blood Cells: | Clear Yellow |
Red, Green or Yellow |
| RT | 26381-01 | Ferric Ammonium Sulfate, 5% Aqueous | 500 ml | 28.00 | Add to Cart |
| RT | 26381-02 | Mayer’s Hematoxylin | 500 ml | 29.00 | Add to Cart |
| RT | 26381-03 | Picric Acid-Orange G Solution | 500 ml | 43.00 | Add to Cart |
| RT | 26381-04 | Ponceau Acid Fuchsin Solution | 500 ml | 29.00 | Add to Cart |
| RT | 26381-05 | Phosphotungstic Acid, 1% | 500 ml | 39.00 | Add to Cart |
| RT | 26381-06 | Aniline Blue Solution | 500 ml | 46.00 | Add to Cart |
Modified Movat’s Stain
For connective tissue stains – Elastic tissue stains.
Stain results:
| Nuclei: | Black | |
| Cytoplasm: | Red | |
| Elastic Fibers: | Purple to Black | |
| Collagen and Bone: | Yellow | |
| Mucopolysaccharides: | Blue-Green | |
| Muscle: | Red |
References:
- Movat, H.Z.;: Demonstration of all connective tissue elements in a single section. Arch Path., 60:289 – 295, 1955
- Roman N., et al: Orcein-hematoxylin in iodized ferric chloride as a stain for elastic tissue. Stain Technology, 42:199 –202, 1967
| RT | 26385-01 | Alcian Blue, 1% | 100ml | 20.00 | Add to Cart |
| RT | 26385-02 | Alkaline Alcohol | 500ml | 31.00 | Add to Cart |
| RT | 26385-03 | Orcein, 0.2% | 250ml | 31.00 | Add to Cart |
| RT | 26385-04 | Hematoxylin Alcoholic, 5% | 250ml | 28.00 | Add to Cart |
| RT | 26385-05 | Ferric Chloride, 10% | 500ml | 21.00 | Add to Cart |
| RT | 26385-06 | Lugol’s Iodine | 500ml | 19.00 | Add to Cart |
| RT | 26385-07 | Woodstain Scarlet-Acid Fuchsin Working Solution | 250ml | 19.00 | Add to Cart |
| RT | 26385-08 | Acetic Acid, 0.5% | 500ml | 15.00 | Add to Cart |
| RT | 26385-09 | Phosphotungstic Acid, 5% | 500ml | 31.00 | Add to Cart |
| RT | 26385-10 | Alcoholic Saffron, 6% | 100ml | 35.00 | Add to Cart |
Goldner’s Trichrome Method
For connective tissue stains – Elastic tissue stain. Stain with a poly-acid step.
Stain results:
| Nuclear chromatin: | Brownish-Black | |
| Cytoplasm: | Bright Red | |
| Erythrocytes: | Orange | |
| Muscle: | Red | |
| Collagen: | Green |
References:
- Luna, Lee G. Histopathologic Methods and Color Atlas of Special Stains and Tissue Artifacts. Johnson Printers, Downerts Grove, IL p151-152, c 1992.
| RT | 26386-01 | Bouin’s Fluid | 500ml | 25.00 | Add to Cart |
| RT | 26386-02 | Weigert’s Iron Hematoxylin A | 500ml | 34.00 | Add to Cart |
| RT | 26386-03 | Weigert’s Iron Hematoxylin B | 500ml | 27.00 | Add to Cart |
| RT | 26386-04 | Ponceau Acid Fuchsin | 100ml | 10.00 | Add to Cart |
| RT | 26386-05 | Acetic Acid, 1% | 500ml | 14.00 | Add to Cart |
| RT | 26386-06 | Phosphomolybdic Acid Orange G Solution | 250ml | 29.00 | Add to Cart |
| RT | 26386-07 | Light Green Stock, 0.2% | 500ml | 29.00 | Add to Cart |
F.4 Silver Methods
Wilder Modification of Bielschowsky’s
Method
Wilder (1935)
For the staining of Collagen and reticulum.
Stain results:
Reticulum Fibers (If beading is observed and/or a dark
background, replace the ammonium hydroxide silver
| Nitrate solution and the reducing solution): | Black | |
| Collagen: | Rose | |
| Other Tissue Elements: | Red |
References:
- Wilder, H.C., Amer. J. Clin. Path.: 11:817, 1935
- Mallory, F.B.: Pathological Techniques, Phil., W.B. Saunders & Co. c. 1938
- AFIP Manual of Hitological Staining Techniques, NY, McGraw-Hill Publ., c. 1968/3rd ed, p. 92
| RT | 26392-01 | Silver Nitrate, 10.2% Aqueous | 250 ml | 86.00 | Add to Cart |
| RT | 26392-02 | Ammonium Hydroxide, Conc. | 500 ml | 13.00 | Add to Cart |
| RT | 26392-03 | Sodium Hydroxide, 3.1% Aqueous | 500 ml | 38.00 | Add to Cart |
| RT | 26392-04 | Formalin, 40%, Neutralized | 500 ml | 11.00 | Add to Cart |
| RT | 26392-05 | Harris Hematoxylin OR |
500 ml | 29.00 | Add to Cart |
| RT | 26392-5A | Van-Gieson Stain | 500 ml | 26.00 | Add to Cart |
| RT | 26392-06 | Nuclear Fast Red (Kernechtrot) | 500 ml | 36.00 | Add to Cart |
| RT | 26392-07 | Phosphomolybdic Acid, 10% Aqueous | 500 ml | 79.00 | Add to Cart |
| RT | 26392-08 | Uranium Nitrate, 1% | 500 ml | 46.00 | Add to Cart |
| RT | 26392-09 | Gold Chloride, 0.2%, Aqueous | 250 ml | 47.00 | Add to Cart |
| RT | 26392-10 | Sodium Thiosulfate, 5% Aqueous | 500 ml | 18.00 | Add to Cart |
Jones Method for Kidney
- Silver Method
For the staining of basement membranes, reticulum fibers, collagen
and nuclei.
Stain results:
| Basement membranes, reticulum fibers: | Black | |
| Nuclei: | Blue | |
| Cytoplasm, collagen, connective tissue: | Pink-Orange |
References:
- Jones, D.B., Amer. J. Path. 27:99 (1951)
- AFIP Manual of Histological Staining Methods, 3rd Ed., Ed. L. Luna: NY: McGraw-Hill Publications, c. 1968, p.97
| RT | 26396-01 | Periodic Acid, 0.5%, Aqueous | 500 ml | 22.00 | Add to Cart |
| RT | 26396-02 | Methenamine Solution, 3%, Aqueous | 500 ml | 25.00 | Add to Cart |
| RT | 26396-03 | Silver Nitrate, 5% Aqueous Solution | 250 ml | 71.00 | Add to Cart |
| RT | 26396-04 | Borate Buffer Working Solution | 500 ml | 29.00 | Add to Cart |
| RT | 26396-05 | Gold Chloride, 0.2%, Aqueous | 250 ml | 47.00 | Add to Cart |
| RT | 26396-06 | Harris’s Hematoxylin | 500 ml | 29.00 | Add to Cart |
| RT | 26396-07 | Alcoholic Eosin Y Staining Solution | 500 ml | 21.00 | Add to Cart |
| RT | 26396-09 | Sodium Thiosulfate, 3%, Aqueous | 500 ml | 17.00 | Add to Cart |
| RT | 26396-11 | Potassium Ferricyanide, 0.5%, Aqueous | 500 ml | 16.00 | Add to Cart |
| RT | 26396-12 | Sodium Metabisulfite, 3%, Aqueous | 500 ml | 26.00 | Add to Cart |
| RT | 26396-13 | Acid Alcohol, 1% | 500 ml | 19.00 | Add to Cart |
| RT | 26396-14 | Ammonia Water, 0.3% | 500 ml | 11.00 | Add to Cart |
F.5 Keratin Stains
Dane’s Method for Prekeratin, Keratin,
and Mucin
Keratin stain method for the staining of keratin, prekeratin, acid mucopolysaccharides and nuclei.
Stain results:
| Acid Mucopolysaccharides: | Blue | |
| Prekeratin and Keranin: | Orange to Red | |
| Nuclei: | Brown |
References:
- Dane, E.T., and Herman, D.L.: Stain Technology, 38:97, 1963.
- AFIP Manual of Histological Staining Methods, 3rd Ed., Ed L. Luna: New York: McGraw Hill Publications, c 1968, p.83.
| RT | 26401-01 | Phloxine Solution, 1%, Aqueous | 500 ml | 24.00 | Add to Cart |
| RT | 26401-02 | Alcian Blue Solution, 0.5% | 250 ml | 52.00 | Add to Cart |
| RT | 26401-03 | Orange G Solution | 500 ml | 36.00 | Add to Cart |
| RT | 26401-04 | Mayer’s Hematoxylin | 500 ml | 46.00 | Add to Cart |
