Cryostat
Section Preparation Aids
CryoJane™ The
Cryostat Frozen Sectioning Aid System
With the CryoJane™ Tape-Transfer System in your existing cryostat, you will be able to routinely obtain flat, thin, wrinkle-free, frozen sections.
- Offers morphological detail equal to paraffin!
- Sections as thin as 2 microns consistently!
- Transfers flat sections!
- No folds or compression in the sections!
- The system can be easily installed in virtually all cryostats!
- Easy to use & cost effective.
TheCryoJane™ Tape -Transfer System achieves frozen sections
of paraffin quality as thin as 2 microns, wrinkle-free, uncompressed,
fully intact and bonded to the microscope slide… in less than
3 minutes. The frozen section is captured on a cold tape window as
it is being cut and is then transferred
to a cold adhesive-coated slide.
The adhesive on the slide is polymerized into a plastic layer, securely anchoring the still frozen section to the slide. The section on the slide is now ready for fixation and/or staining.
What are the CryoJane’s™ Special Features?
- Thin sections help clarify structural relationships and reveal morphologic detail.
- Flat two micron sections are excellent for fast immuno-stains, in-situ hybridization, IS-PCR, laser capture micro-dissection (LCM), tissue microarrays, and other molecular diagnostic protocols.
- The CryoJane™ method assures that tissue sections can be maintained un-thawed EVEN AFTER MOUNTING. Therefore, sections can be freezedried in the cryostat in about ten minutes or freeze-substituted in as little as ten seconds and then fixed "anhydrously" to preserve virtually all fine structure present in the snap-frozen block of tissue.Water-soluble enzymes, antigens, and nucleo-proteins are also preserved in-situ, and with appropriate fixation and staining, true localization of enzyme and antigen activity can be visualized.
- The bond between sections and slide is resistant to proteases, alkalis, and acids. No longer will you lose sections after cytochemical digestion steps, or after rigorous protocols such as in-situ hybridization or IS-PCR.
Why are frozen sections generally so inferior to paraffin sections and how does CryoJane™ get around these problems?
- When a cut section is mounted on a room temperature slide, the flow of the melting ice causes water-soluble components of the cell to be displaced by the flow. In the CryoJane Tape-Transfer process, sections are cut, transferred and tightly bonded to the microscope slide without ever being permitted to melt.
- Slow freezing produces large ice-crystals, which damage insoluble structural elements and causes displacement of water-soluble components. In the CryoJane process, the tissue is snap-frozen to minimize ice crystal size.
- When unfixed wet or dry sections are placed in aqueous solutions (even aqueous fixatives) water-soluble substances wash out of the sections.
- We developed an aqueous fixative that maintains the structural
elements in the cell, producing H&E stained slides of high quality.
An alternative method is free-substitution where the ice in the frozen
sections is dissolved (not melted) in a cold (-35ºC) solvent,
yielding"dry" sections, which can then be
fixed appropriately. Free-substitution yields the best morphological results.
CRYOJANE™ System
includes the following:
Flash-Pad Mechanism (1); Electronics Control Unit (2); Power Cord (3); Door Channel (4); Storage Shelf (5); Hand Roller (6); 125 Adhesive- Coated Slides (7); 400 Tape Windows (8); 2 Knife Facet Wipers (9); Spare Fuse; and 5 Block Holders (optional)
Notes: System does not include: Snap Freezer, oil bath or oil embedding accessories
Products required by the CryoJane System
Protective
Oil Accessories Kit
Oil in the bath is maintained at –8ºC inside the cryostat and will not melt the tissue when brushed on the frozen block-face. Frozen blocks coated with this protective oil can be stored for up to one year in a freezer without dehydration.
The Protective Oil Accessories Kit includes the following: Temperature controlled Oil Bath, 4 oz Bottle of Protective Oil, and Oil Brush. Please note that installation of the Protective Oil Bath Accessory requires that the CryoJane™ system be returned to Electron Microscopy Sciences.
Accessories Notes
| Cat. # | Description | Application Notes |
| 62800-40 | Block-Holder | Deep-waffled, large face block-holder securely anchors frozen embedding medium or tissue. |
| 62800-44 | Microtome Chuck Adapter | Adapts to the microtome chuck for the use of Block-Holders above. (Please specify cryostat model when ordering.) |
| 62800-46 | Hand Roller |  Required in the CryoJane™ process for laminating Tape
Window to block-face. |
| 62800-48 | Storage Shelf | Used for storing Adhesive Coated Slides and Tape Window inside the cryostat. |
| 62800-50 | Door Channel | Please specify model cryostat and whether Cryo-Vac-Away is installed. |
| 62800-53 | Fuses - 1-Amp | SLO-BLOW ceramic core wound. Used in the CryoJane™ ECU. |
| 62800-X | Adhesive Slides | Glass slide with adhesive coating, when polymerized, bonds the still-frozen section from the tape to the slide. Avoid thawing the section. ½ x coating slides for mild to normal tissues. |
| 62800-1X | Adhesive Slides | Glass slide with adhesive coating, when polymerized, bonds the still-frozen section from the tape to the slide. Avoid thawing the section. 1x coating slides for mild to normal tissues. |
| 62800-4X | Adhesive Slides | Glass slide with adhesive coating, when polymerized, bonds the still-frozen section from the tape to the slide. Avoid thawing the section. 4x coating slides for use with hard un-calcified bone) or fatty tissues. |
| 62800-72 | Tape Windows | Applied to the block-face with the Hand Roller to capture the frozen section as it is being cut. Eliminate the need for a brush or anti-roll device. |
| 62800-74 | Protective Oil | Used for coating frozen section blocks to prevent dehydration during long-term storage. |
| 62800-76 | Aqueous Buffer Salt Mix | Used in the preparation of the aqueous fixative, which preserves morphological detail in frozen sections. 2 vials per package, (each vial makes 180 ml of buffer). |
| 62800-78 | Knife Facet Wipers | Effectively cleans both facets of knife-edge without dulling the blade. 10 wipers/pack |
CryoJane™ System Ordering
| Cat. # | Description | Pack | Price | |
| 62800-10 | CryoJane™ Tape-Transfer System, 110Volt | each | 6700.00 |  |
| 62800-20 | CryoJane™ Tape-Transfer System, 220Volt | each | 6700.00 | |
| 62801-10 | CryoJane™ Tape-Transfer System, 110V with 5 Block Holder (#62800-40) | each | 6730.00 | |
| 62803-30 | *Protective Oil Accessories Kit | each | 260.00 | |
*Installation of the Protective Oil Bath Accessory requires that the CryoJane™ system be returned to Electron Microscopy Sciences.
CryoJane™ Accessories and Supplies Ordering
| 62800-40 | Block-Holder | 5/pk | 150.00 | |
| 62800-44 | Microtome Chuck Adapter (Please specify cryostat model when ordering.) | each | 168.00 | |
| 62800-46 | Hand Roller | each | 50.00 | |
| 62800-48 | Storage Shelf | each | 32.00 | |
| 62800-50 | Door Channel (Please specify model cryostat and whether Cryo-Vac-Away is installed.) | each | 50.00 | |
| 62800-53 | Fuses - 1-Amp | 5/pk | 19.50 | |
| 62800-X | Adhesive Slides - ½ x Coating | 125/pk | 175.00 | |
| 62800-1X | Adhesive Slides - 1x Coating | 125/pk | 175.00 | |
| 62800-4X | Adhesive Slides - 4x Coating | 100/pk | 175.00 | |
| 62800-72 | Tape Windows | 400/pk | 50.00 | |
| 62800-74 | Protective Oil | 4oz | 25.00 | |
| 62800-76 | Aqueous Buffer Salt Mix | 2/pk | 32.50 | |
| 62800-78 | Knife Facet Wipers | 10/pk | 10.00 | |
Cryogenic-Gel
- Replaces the messy "runny" embedding media.
- Easy to use.
- Stick to where you want it.
- Will not run – No waste
It is a highly viscous, water-soluble embedding medium for frozen sections that "stays put". The gel is packaged in a pump-dispenser bottle that can instantly dispense the gel in any position. The dispenser has a built-in"guillotine" that cleanly severs the gel from the pump spouts and prevents any "stringy" medium from pulling away when the bottle is removed. Simply dispense gel on room temperature blockholder. Use cold heat extractor to form a base layer. Dispense additional gel. Place the tissue on top of gel. Use cold heat extractor to freeze tissue and form block. Each bottle contains 4.3oz (159ml), 12/case.
| 62806-01 | Cryo-Gel™, 4.3oz (128ml) per Dispenser | each | 12.00 | |
| 62806-06 | Cryo-Gel™, 4.3oz (128ml) per Dispenser | 12/cs | 126.00 | |
CureMount™ -
Mounting Medium
CureMount™ is a UV curable mounting medium of low viscosity for use with all dehydrated sections. CureMount™ is a polymer that cures to a refractive index of 1.55. It closely matches the refractive index of dehydrated tissue. The coverslip can be optimally positioned and cured under UV fluorescent illumination. The coversliped slide is "dry" in less than 30 seconds. The coverslip can easily be removed by heating the slide briefly, soon after curing or after a longer time by immersing in xylene. All unstained tissue elements and the cured polymer will be invisible, making the stained elements stand out in exquisite detail. Comes in brown glass dropping bottle, 60 ml.
The CureMount™ Starter Kit contains: One CureMount™ Curing Lamp and two bottles of CureMount™.
| 62804-01 | CureMount™ I, Mounting Medium for Frozen Section | 60ml | 29.95 | |
| 62804-02 | CureMount™ II, Mounting Medium for Paraffin Section | 60ml | 29.95 | |
| 62804-50 | CureMount™ Curing Lamp | each | 69.95 | |
| 62804-52 | CureMount™ Starter Kit | each | 125.00 | |
Cryo-Vac-Away
System
The Cryo-Vac-Away adapts to virtually all cryostats. A nozzle at the block-face removes trimming debris as it is generated. The debris is trapped in the Cryo-Vac-Filter and pathogens are captured in the Bacterial-Cryo-Filter inside the cryostat, substantially reducing the biohazard risks associated with infectious material. The cryostat remains clean without unwanted materials.
The Cryo-Vac-Away system includes the following:
Vacuum Blower (1); Foot Switch (2); Swivel Assembly (3); Swivel Assembly Mounting Bracket (4); Collection Nozzle (5); Vacuum Hose (6); Vacuum Hose Adapter Tube (7); Hose Fitting (8); Hose Clamp; Door Channel; 1 Cryo-Vac-Filter; and 1 Bacterial Cryo-Filter.
Notes:
- To fit properly the Cryo-Vac-Away to your Cryostat, please specify your Cryostat model and make.
- If you are planning to purchase a new cryostat, contact EMS. We may able to offer you a Cryostat/CryoJane/Cryo-Vac-Away package.
Cyro-Vac-Away System Ordering
| Cat. # | Description | Pack | Price | |
| 62810-10 | Cryo-Vac-Away System, 110V | each | 850.00 | |
| 62810-20 | Cryo-Vac-Away System, 220V | each | 850.00 | |
| 62810-30 | Bacterial Cryo-Filters, 12 Filters/case | 12/cs | 100.00 | |
| 62810-35 | Cryo-Vac-Away Filter, 12 Filters/case | 12/cs | 130.00 | |
Cryo-Vac-Away Replacement Parts
| 62810-40 | Vacuum Hose Adapter Tube (specify Model Cryostat) | each | 60.00 | |
| 62810-45 | Vacuum Blower | each | 150.00 | |
| 62810-47 | Hose Clamps, 4 clamps/pack | 4/pk | 20.00 | |
| 62810-50 | Hose Fittings, Right Angle, 10/pk | 10/pk | 50.00 | |
| 62810-55 | Vacuum Hose Set (Specify Model Cryostat) | Set | 20.00 | |
| 62810-60 | Foot Switch | each | 50.00 | |
| 62810-63 | Swivel Assembly (Nozzle and Bracket Not Included) | each | 250.00 | |
| 62810-65 | Mounting Bracket (for Swivel Assembly) | each | 40.00 | |
| 62810-68 | Collection Nozzle (Specify Model Cryostat) | each | 50.00 | |
Snap-Freezer
- Stand- Alone Gentle Jane (SAGJ)
The Stand-Alone Gentle Jane is portable (10 lbs, 12"x12"x12") and enables the user to snap-freeze tissue in 8-10 seconds. The heat Extractor, chilled in a refrigerant (i.e. liquid nitrogen), is placed on the Gentle Jane device and drops at a controlled rate snap freezing the tissue and embedding medium. Frozen blocks can be stored inside the SAGJ chamber for up to 2 hours. The SAGJ includes the following:
- Stand-Alone Gentle Jane (SAGJ) insulated chamber;
- Gentle Jane Device
- Heat Extractor; Plastic Thermos
- Stand-Alone Gentle Jane (SAGJ) insulated chamber;
- Gentle Jane Device;
- Heat Extractor; Plastic Thermos
- 5 Block Holders
Note: Some models Cryostats require a Microtome Chuck Adapter in order to use Block Holder.
Ordering SAGJ
| 62830-10 | Stand-Alone Gentle Jane – Snap Freezer (SAGJ) If purchased separately from the CryoJane™ System (#62800-10) | each | 985.00 | |
| 62831-10 | Stand-Alone Gentle Jane – Snap Freezer (SAGJ) If purchased with CryoJane System (#62800-10) | each | 675.00 | |
Snap Freezing Hardware and Accessories
| 62830-40 | Gentle Jane Device - Black anodized aluminum device with a controlled drop mechanism. Heat Extractor fits in U-shaped portion. Block Holder fits on post. | each | 400.00 | |
| 62830-45 | Heat Extractor - 3⁄4 lb (340g) chrome-plated brass weight, chilled and used with Gentle Jane Device to snap-freeze tissue and embedding medium. | each | 75.00 | |
| 62830-48 | Plastic Thermos - Used to hold the Heat Extractor in a freezing agent (i.e. LN2) in the Gentle Jane Snap Freezer. (Heat Extractor not included) | each | 15.00 | |
