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Electron Microscopy Sciences

Histology and Light Microscopy

arrow14Cryo-Embedding

arrow10Precision Cryo-Embedding System

This section offers you a simple system of stainless steel well bars, chucks and freezing blocks, kept cold in cryostat, allow you to perform a variety of precision embedding techniques addressing the most difficult embedding tasks with ease and minimum time needed.

Speed

  • 20 to 60 seconds to freeze (depending on the well size)
  • Multiple samples handled quickly
  • No monitoring necessary (for placement of heat extractor)

Precision

  • Precise flat or on edge embedding
  • Predictable orientation
  • Liquid samples easily embedded
  • No crush artifact caused by heat extractor weight

Reduced Tissue Waste

  • Less trimming the result of flat plane of embedding
  • Minute samples embedding in the same plane.

Easy and Comfortable

  • Less cryostat adjustments
  • Comfortably preformed on a shelf just inside most cryostats
  • Easy to used – and safe time

arrow10 1. Embedding Wells

61710-18 Embedding Bar, 4-Well of 18 x 18mm Embedding Bar each 250.00 Add to Cart
61710-24 Embedding Bar, 4 well of 24 x 24 mm Embedding Bar each 250.00 Add to Cart
61710-30 Embedding Bar, 3 well of 30 x 30 mm Embedding Bar each 250.00 Add to Cart
61712-34 Embedding Bar, 2 wells of 30 & 24 mm Embedding Bar each 265.00 Add to Cart
61713-50 Embedding Bar, 2 well of 50 x 30 mm completely fills a standard glass slide Embedding Bar each 250.00 Add to Cart
61714-20 Embedding Bar, 20 x 20 mm, 9mm Deep Wells Embedding Bar each 250.00 Add to Cart
61715-24 Embedding Bar, 24 x 24 mm Deep Wells Embedding Bar each 250.00 Add to Cart
61716-10 Embedding Bar, 10 x10 mm Wells Embedding Bar
each 250.00 Add to Cart

Stainless Steel Chucksarrow10 2. Stainless Steel Chucks

These stainless steel chucks are designed to be used cold for rapid freezing, and to maximize the gripping power required to hold the embedded tissue block. The stem of the chuck provides the focal point for a sharp tap resulting in an easily release of the block from the well. These chucks fit many of the major brand cryostat and can be used in most cryostats with use of an adapter. These Chuck Adapters have stem measures 20mm L x 9mm diameter, and fit Leica and most Microm cryostats. We also offer adapters to use these chuck for other cryostats.

61720-28 Small Chuck – Made of stainless steel, 28 mm square with gridded surface each 59.00 Add to Cart
61720-36 Large Chuck – Made of stainless steel, 36 mm square with gridded surface each 69.00 Add to Cart
61721-36 Large Chuck – Stainless steel, 36 x 28 mm rectangular with gridded surface, for use with Bar Well #61711-32 each 72.00 Add to Cart
61721-55 Extra Large Chuck – Stainless steel 55 x 36 mm rectangular shape each 80.00 Add to Cart

Stainless Steel Over Chucks Freezing Blocksarrow103. Stainless Steel Over Chucks Freezing Blocks

Thee stainless steel rectangular blocks are a useful tool for a number of tasks. They serve as a heat extractor, designed to fit over the stem of the chuck. The flat surfaces can also be used as an extra heat extractor when using conventional cryostat embedding technique. The freezing block also served as a dislodging tool. A light tap of the chuck stem cleaves the plane of adhesion holding of the formed block to the well. These blocks also serve as a convenient hand held flat freezing surface useful in gently cooling the tissue without the use of sprays and in plastering technique.

61725-01 Over-Chuck Freezing Block each 24.00 Add to Cart

Chucks Binarrow10 4. Chucks Bin

A sturdy stainless steel bin to store over-chuck freezing block while staying cold in cryostat. Removable cover for easy access the contents.

61725-50 Chuck Storage Bin each 26.00 Add to Cart

Dispensing Slides transparent polyvinylarrow105. Dispensing Slides

These thin transparent polyvinyl slides serve as a surface to precisely orient the tissue into the desired position and as a means to accurately transfer tissue to the embedding well floor. Tissue is applied face down to the end of the dispensing slide where it can be visualized from below and manipulated into position. The face that is visible will be laid onto the well floor and will ultimately be the embedded surface to be sectioned.

61727-08 Dispensing Slide 8/pk 22.00 Add to Cart

Embedding Shelf light microscopyEmbedding Shelfarrow106. Embedding Shelf

This removable shelf can be installed below the opening of the cryostat in the most convenient and location available. The selves can be made in various sizes to accommodate most instruments.

Also available two more models of 3 pieces shelf size 4 x 8” for temporary or permanently installation. One for Leica or Micron cryotats, and one for Shandon Cryostat

61728-01 Embedding Shelf, size 4 x 8” each 100.00 Add to Cart
61728-LM Embedding Shelf for Leica/Micron each 195.00 Add to Cart
61728-Sh Embedding Shelf for Shandon each 195.00 Add to Cart

Laica cryostat well bararrow10 7. Well Bar

Storage Platform

This stainless steel plate to fit to the Laica cryostat for storing Well Bar inside the cryostat

61730-01 Well Bar  Storage Platform esch 55.00 Add to Cart

Chuck Adapter Ames/Tissue-TekChuck Adapter Shandon arrow10 8. Chuck Adapter

This Chuck Adapter machined to fit Shandon and Ames / Tissue-Tek cryostats.

61731-01 Chuck Adapter for Shandon each 110.00 Add to Cart
61732-01 Chuck Adapter for Ames/Tissue-Tek each 110.00 Add to Cart

Frozen Section Brushesarrow109. Frozen Section Brushes

These flat white boar bristle brush which is cut into an angle. This stiffness bristle will prevent the frozen section from curling through the brush. We offer a set of four brushes: two #1 with 3⁄8” angle cut and #2 with 1⁄4” angle cut.

61735-01 Frozen Section Brush 4/set 20.00 Add to Cart

arrow10Well Bar Sets

We offer these Well Bar Sets to make your ordering easier and to offer you a complete set for facilitate your work:

arrow10Well Bars Set 101

Includes: Three sizes well bars (61710-18, 61710-24, 61710-30); Six small chucks (6 x 61720-28); Four large chucks (4 x 61720-36); Four Over-Chuck Freezing Blocks (4 x 61725-01); One Chuck Bin (1 x 61725-50); 16 Dispensing Slide (61727-08); Cutting Board/Freezing Girdle (61733-01); 1 Elevated Freezing Block (61734-01); 1 Angle Embedding Forceps (78359-124); and accessories

61700-01 Well Bars Set 101 set 1840.00 Add to Cart
arrow10Well Bars Set 102

This set is dedicated for Ames / Tissue-Tek Cryostats.

Includes: Three sizes well bars (61710-18, 61710-24, 61711-32); Six small chucks (6 x 61720-28); Four large chucks, 36x28mm (4 x 61721-36); One Chuck Adapter (61732-01); Four Over-Chuck Freezing Blocks (4 x 61725-01); One Chuck Bin (1 x 61725-50); 16 Dispensing Slide (61727-08); Cutting Board/Freezing Girdle (61733-01); 1 Elevated Freezing Block (61734-01); 1 Angle Embedding Forceps (78359-124); and accessories

61701-01 Well Bars Set 102 set 1840.00 Add to Cart
arrow10Small Embedding Well Bar Set

Includes: One 18 mm Well Bars (61710-18); Four Small Chucks (61720-28); Two Over Chuck Freezing Blocks (61725-01); Dispensing Slides (61727-08); Accessories included.

61704-01 Small Embedding Well Bar Set 550 550.00 Add to Cart
arrow10Medium Embedding Well Bar Set

Includes: One 24 mm Well Bar (61710-24); Four Small Chucks (61720-28); Two Over Chuck Freezing Blocks (61725-01); Dispensing Slides (61727-08); Accessories included.

61704-02 Medium Embedding Well Bar Set set 550.00 Add to Cart
arrow10Large Embedding Well Bar Set

Includes: One 30 mm Well Bars (61710-30); Four Small Chucks (61720-36); Two Over Chuck Freezing Blocks (61725-01); Dispensing Slides (61727-08); Accessories included.

61704-03 Large Embedding Well Bar Set set 590.00 Add to Cart
arrow1024/30 mm Well Bar Set

Includes: One Well Bar with two wells 24 mm and two wells 30mm (61712-34); Two Large Chucks (61720-36); Two Small Chucks (61720-28); Two Over Chuck Freezing Blocks (61625-01); 8 Dispensing Slides (61727-08)

61704-04 24/30 mm Well Bar Set set 585.00 Add to Cart
arrow1050/30 mm Well Bar Set

Includes: One Well Bar 50 x 30 mm (61713-50); Two Large Chucks (61720-36) Two Small Chucks 55 x 36 mm (61721-55)

61704-05 50/30 Well Bar Set set 410.00 Add to Cart
arrow1020 x 20 mm Deep Well Bar Set

One 20 x 20 mm Deep Well Bar (61714-20); Two Small Chucks (61720-28); Two Over Chuck Freezing Blocks (61725-01); 8 Dispensing Slides (61727-08)

61704-06 20 x 20 mm Deep Well Bar Set set 550.00 Add to Cart

Cutting board / Freezing griddlearrow10Freezing Griddle/ Elevated Freezing Block Set

Includes:

Cutting board / Freezing griddle: The steel “freezing griddle” serves as a freezing surface on which to prepare frozen blocks when used in conjunction with the elevated freezing block. The small attached cutting board serves as a cold surface to cut the frozen block while maintaining the freezing temperature. This simple piece of apparatus is kept cold in the cryostat and is removed to the work bench only during the process of cutting the frozen block. As sections are cut, they are kept cold on the freezing griddle.

Elevated Freezing Block: This steel block is kept cold in the cryostat and in used in conjunction with the freezing griddle. The two broad sides of the block are equipped with feet measuring 3.0 mm on one side and 4.5 mm on the other. The feet create a space between the two steel bars in which the frozen blocks are prepared. Additional elevating bars are supplied to further elevate the block when preparing very thick portion of tissue.

Epoxy Coated Forceps

Putty knife and extra elevating bars

61706-01 Freezing Griddle/ Elevated Freezing Block Set each 290.00 Add to Cart

Cytology Funnel Sample Chambers

Cytology Funnel Sample Chambers arrow12