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Electron Microscopy Sciences

Histology and Light Microscopy

arrow13Prepared Staining Kits for Histology and Pathology

G. CYTOPLASMIC GRANULE STAINS

G.1 Pancreatic Islet Cell Stains

technical data sheetarrowMallory-Heidenhain Azan-Gomori's Modification, Islet Cells

For the staining of alpha, beta and D-cells of the islets of langerhans.

Stain results:

(Bouin's Fixation)

  Human Tissue Guinea Pig
Alpha granules: Red, bright Orange - Tan
Beta granules: Orange-Brown, dull Red, fiery
D-cell granules: Dark Blue Deep Blue

References:

  • Clark, G, (ed): Staining Procedures 3rd Ed.; Baltimore: Williams & Wilkins Co., c. 1973, p. 171
  • Gomori, G.: "Studies on the cells of the Pancreatic islets." Anat. Rec. 74:439 (1939)
RT 26450-01 Azocarmine Staining Solution, 0.1% msds 250 ml 34.00 Add to Cart
RT 26450-02 Aniline-Alcohol, 1% Poison Pack msds 250 ml 17.00 Add to Cart
RT 26450-03 Ferric Ammonium Sulfate, 5%, Aqueous msds 500 ml 17.00 Add to Cart
RT 26450-04 Aniline Blue – Orange G Solution msds 500 ml 29.00 Add to Cart
RT 26450-05 Orange G, 0.2% in Alcohol msds 500 ml 32.00 Add to Cart

technical data sheetarrowMaldonado's Method For Pancreatic Islet Cells

For the staining of alpha, beta, D- and Exocrine cells of the islets of langerhans.

Stain results:

(Bouin's Fixation without Acetic Acid)

  Human Tissue
Alpha granules: Purple
Beta granules: Violet Blue
D-cell granules: Light blue with evident granules
Exocrine cells: Grayish blue with red granules

References:

  • Maldonado, R. and San Jose, H. Stain Techn., 42:11-13, 1967
  • Luna, L.G., (ed.), Manual of Histologic Staining Methods of the AFIP, 3rd edition, McGraw, N.Y. p. 105, c. 1968
RT 26458-01 Phloxine 1% Aqueous Solution msds 500 ml 28.00 Add to Cart
RT 26458-02 Phosphotungstic Acid, 3% Aqueous Solution msds 500 ml 50.00 Add to Cart
RT 26458-03 Azure II Solution, 0.05% msds 500 ml 16.00 Add to Cart
RT 26458-04 Weigert's Iron Hematoxylin A msds 500 ml 27.00 Add to Cart
RT 26458-05 Weigert's Iron Hematoxylin B msds 500 ml 21.00 Add to Cart

G.2 Mast Cell Stains

technical data sheetarrowToren's Method for Mast Cells

Cytoplasmic granule stains: Mast cell stains.

Stain results:

Mast Cell Granules:   Deep Blue
Collagen & Cartilage:   Blue-Green
Muscle &Elastic Fibers:   Red
Fibrin:   Purple
Bone:   Green
Colloid, Keratin & Erythrocytes:   Orange Red to Orange

References:

  • Toren, D.A.: Stain Techn., 38:249-250, 1963
  • Luna, L., (ed.), Manual of Histologic Staining Methods of the AFIP, 3rd. ed., McGraw-Hill, New York, c. 1968, pp. 116-117
RT 26472-01 Giemsa Stain Solution msds 500 ml 63.00 Add to Cart
RT 26472-02 Acetic Acid, 0.5%, Aqueous   500 ml 16.00 Add to Cart
RT 26472-03 Pollack's Trichrome Solution in 50% Alcohol, 3A msds 100 ml 30.00 Add to Cart

G.3 Other Granule Stains

technical data sheetarrowMethenamine Silver Methodfor Argentaffin Cells

For the staining of argentaffin granules of enterochromaffin cells.

Stain results:

Argentaffin Cells:   Black
Coarse connective tissue of the submucosa:   Variable amount of
blackening by 3–31 ⁄2 hr. of impregnation
Granules of eosinophil leukocytes, nuclei,
smooth muscle & surface epithelium:
  show additional blackening
after incubation beyond 3–31 ⁄2 hr.
Granules of mast cells:   Remain red after nuclei &
reticulum are blackened

References:

  • Clark, G.: Staining Procedures, Williams and Wilkins Co., Baltimore, 3rd Ed., c. 1973, p. 161.
  • Gomori, G., Arch. Path., 45:48, 1948.
  • Burtner, H.J. and Lillie, R.D., Stain Techn., 24:225-7, 1949.
RT 26481-01 Weigert's Iodine Solution msds 500 ml 29.00 Add to Cart
RT 26481-02 Sodium Thiosulfate, 5%, Aqueous msds 500 ml 21.00 Add to Cart
RT 26481-03 Silver Nitrate, 5%, Aqueous msds 250 ml 86.00 Add to Cart
RT 26481-04 Methenamine, 3%, Aqueous msds 500 ml 28.00 Add to Cart
RT 26481-05 Borate Buffer, pH 7.8   500 ml 21.00 Add to Cart
RT 26481-06 Gold Chloride, 0.1%, Aqueous   250 ml 31.00 Add to Cart
RT 26481-07 Saffranin O Solution, 0.1%   500 ml 30.00 Add to Cart

Fat and Lipid Stains arrow13arrow13