Electron Microscopy Sciences

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Histology and Light Microscopy

Electron Microscopy Sciences

arrow14 Reagents for Histology: Fixing, Dehydrating, Clearing and Staining

arrow12HISTOCHOICE™MB® *- The Molecular Biology Fixative

  • SAFE - Contains no Formaldehyde, Glutaraldehyde or Mercury.
  • NON-TOXIC – Is completely odorless and can be safely disposed
    of down the drain.

HISTOCHOICE™ MB® Tissue Fixative is the first fixative designed for the molecular biologist. It is specialty formulated to preserve antigenic sites for antibody probes and nucleic acid sites for immunohistochemistry. HISTOCHOICE™MB® replaces formaldehyde based, alcohol based, Zenkers, B-5, B-3, Bouin’s and other fixatives with superior results. Tissue fixed in HISTOCHOICE™MB® exhibit vibrant staining, better nuclear and cytoplasmic detail, and will retain a crisp appearance even after long-term fixation.

Because this formula is designed specially for molecular biology applications, HISTOCHOICE™MB® fixed sections do not require pre-digestion or other recovery procedures to make important sites available.This means you spend less time preparing slides and more time doing research.

More than a fixative, HISTOCHOICE™MB® is a preservative that leaves antigens and nucleic acids in their native state, allowing binding for specific probes. Primary antibodies can often be diluted several-fold due to the increased number of preserved antigenic sites, saving cost
on every slide you process.

HISTOCHOICE™MB® fixed tissue retains a much more natural look and feel than formalin fixed tissue. It is important to remember that these tissues are not fixed by formalin, but rather preserved by HISTOCHOICE™MB®.

RT 64115-01 HISTOCHOICE™MB® Fixative 1 L 46.00 Add to Cart
RT 64115-04 HISTOCHOICE™MB® Fixative 4 L 150.00 Add to Cart
RT 64115-44 HISTOCHOICE™MB® Fixative 4x4 L 495.00 Add to Cart

* HISTOCHOICE is registered name of Amresco

msdsarrow12Histo-Clear®/Histo-Clear II®

Histo-Clear® (Distilled essential oils –food grade) and Histo-Clear II® (A mixtureof Aliphatic Hydrocarbon and Distilled essential oils – food grade, reduced citrus odor) are excellent non-toxic clearing agents replacing Xylene.

RT 64110-01 Histo-Clear® 1 L 24.00 Add to Cart
RT 64110-04 Histo-Clear® 1 gal 100.00 Add to Cart
RT 64110-10 Histo-Clear® 5 gal 420.00 Add to Cart
RT 64111-01 Histo-Clear II® 1 L 18.00 Add to Cart
RT 64111-04 Histo-Clear II® 1 gal 68.00 Add to Cart
RT 64111-10 Histo-Clear II® 5 gal 279.00 Add to Cart

® is register name of National Diagnostics

arrow12Hollande’s Fixative

A modification of Bouin's solution. It is stable and will decalcify small bone specimens. Tissue that is fixed with Hollande's can be stained successfully with most stains, and the cupric acetate in the solution stabilizes red blood cell membranes and cosinophil and endocrine cell granules so that less lysis occurs than with Bouin's solution. Hollande's is widely used as a fixative for biopsy specimens of the gastrointestinal tract.

Thorough washing of the fixative prior to placing the specimen in a phosphate buffered formalin solution is necessary because the salts present in the solution will form an insoluble phosphate precipitate. Packaged in leak-proof histo-containers; prefilled ready-to-use.

  Catalog # Cup Size Filled Pack/cs Price  
RT 64120-10 15 ml 10ml 50 76.00 Add to Cart
RT 64120-20 30 ml 20ml 50 96.00 Add to Cart
RT 64120-30 60 ml 30ml 20 44.00 Add to Cart

Bulk packaging is available:

RT 64120-01 Hollande's Fixative 1 L 41.00 Add to Cart
RT 64120-04 Hollande's Fixative 4 L 130.00 Add to Cart

arrow12Hydrochloric Acid, Diluted, (w/v)

HCl F.W. 36.46 CAS 7647-01-0

RT 16762 Hydrochloric Acid, 1% Aqueous 500 ml 17.00 Add to Cart
RT 16763 Hydrochloric Acid, 2% Aqueous 500 ml 18.00 Add to Cart
RT 16765 Hydrochloric Acid, 10% Aqueous 500 ml 27.00 Add to Cart
RT 16766 Hydrochloric Acid, 20% Aqueous 500 ml 33.00 Add to Cart

arrow12Hydrogen Peroxide 30% Solution- Stabilized, Reagent, A.C.S.

H2O2   F.W. 34.01   CAS #7722-84-1

Hydrogen Peroxide, 30% 16790 msds
Hydrogen Peroxide, 3% 16793 msds
Assay   29-32%
Maximum of Impurities:    
Chloride(Cl)   0.0003%
Nitrate   0.0002%
Phosphate   0.0002%
Sulfate   0.0005%
Heavy Metal   0.0001%
Iron   0.00005%
Titratable Acid   0.0006meq/g
RT 16790   100ml 38.50 Add to Cart
RT 16792   450ml 130.00 Add to Cart
0-4ºC 16793 Hydrogen Peroxide, 3% Aqueous 500 ml 47.00 Add to Cart

arrow12Hydroquinone Aqueous Solution

0-4ºC 16795-1 Hydroquinone, 1% 500 ml 35.00 Add to Cart
0-4ºC 16795-2 Hydroquinone, 2% 500 ml 37.00 Add to Cart
0-4ºC 16795-4 Hydroquinone, 4% 500 ml 41.00 Add to Cart

msdsarrow12Isopropyl Alcohol, Reagent, A.C.S.

(2-Propanol)
CH3CH(OH)CH3   F.W. 60.10   CAS #67-63-0

A.C.S. Specifications:

Color (APHA) . 10
Density @ 25°C . 0.781-0.783
Boiling Range (including 82.3°C) . 1°C
Titratable Acid or Base . 0.0001 meq/g
Residue after Evaporation . 0.001%
Solubility in water . To pass test
Water . 0.2%
RT 16930 1qt 12.00 Add to Cart
RT 16931 4x1qt 42.00 Add to Cart

arrow12McDowell Trumps Fixative

RT 18030-05 McDowell Trumps Fixative 500 ml 30.00 Add to Cart
RT 18030-10 McDowell Trumps Fixative 1 L 36.00 Add to Cart

arrow12Michel’s Buffer

RT 18041-05 Michel’s Buffer 500 ml 19.00 Add to Cart
RT 18041-10 Michel’s Buffer 1 L 25.00 Add to Cart

arrow12Michel’s Fixative, pH 7.0 – 7.4

RT 18040-05 Michel’s Fixative 500 ml 46.00 Add to Cart
RT 18040-10 Michel’s Fixative 1 L 81.00 Add to Cart
RT 18040-20 Michel’s Fixative Pre-filled 20 x10 ml 71.00 Add to Cart

arrow12Methanol, Reagent, A.C.S.

(Methyl Alcohol)
CH3OH   F.W. 32.04   CAS #67-56-1

Assay:   99.8%
Specific Gravity :   0.79

A.C.S. Specifications:

Residue after Evaporation . 0.001%
Boiling Range (Boiling point 64.6°C) . 2°C max
Appearance . Clear
Color (A.P.H.A.) . 10 max
Acetone & Aldehydes (0.001%) . To pass test
Titratable Acid . 0.0003 meq/g
Titratable Base . 0.0002 meq/g
Water . 0.2% max
RT 18510 1qt 15.00 Add to Cart
RT 18511 4x1qt 54.00 Add to Cart

msdsarrow12Periodic Acid, Reagent

H5IO6   F.W. 227.96   CAS #10450-60-9

Assay   >99.0%
Heavy Metals   .0.005%
Insoluble Matters   0.01%
Iron   .0.003%
Other Halogen (Cl)   0.01%
Residue after Ignition   0.01%
Sulfate   0.01%
  • Ainsworth, S.K. et al. (1972). Alkaline bismuth reagent for high resolution ultrastructural demonstration of periodic-reactive sites. J. Histochem. Cytochem., 20:995.
  • Mowry, R.W. (1958). Improved procedure for the staining of acid polysaccharides by Muller’s colloidal (hydrous) ferric oxide and its combination with the Feulgen and the periodic acid Schiff
    reaction. Lab. Invest., 7:566
  • Mowry, R.W. (1959). Effect of Periodic Acid used prior to Chromic Acid on the staining of Polysaccharides by Gomori’s methenamine silver. J. Histochem. Cytochem., 7:288
  • Tsuchiya, A. and Ogawa, K. (1973). Ultracytochemistry of the Periodic Acid (PA) - Phosphotungstic Acid (PTA) reaction. J. Electr. Microsc., 22:290
  • Hanker, J.S. et al (1964). Osmiophilic reagents: new cytochemical principles for light and electron microscopy. Science New York, 146:1039
  • Derenzini, M. et al (1986). An improved periodic acid-thiosemicarbazide- osmium technique to reveal glycoconjugates at the molecular level in-situ. J. Histochem. Cytochem., 34:1161
RT 19325   25 g 30.00 Add to Cart

msdsarrow12Periodic Acid Aqueous Solutions

  19324-05 Periodic Acid 0.5% Aqueous 500 ml 24.00 Add to Cart
  19324-10 Periodic Acid 1% Aqueous 500 ml 27.00 Add to Cart
  19324-50 Periodic Acid 5% Aqueous 500 ml 32.00 Add to Cart

arrow12Phosphate Buffer, 0.1M*

Prepared from Sodium Phosphate Monobasic Hydrate and Sodium Phosphate Dibasic Heptahydrate. Non-Sterile. Meant for research purposes only.

  19340-60 Phosphate Buffer, pH 6.0 1 L 34.00 Add to Cart
  19340-65 Phosphate Buffer, pH 6.5 1 L 34.00 Add to Cart
  19340-70 Phosphate Buffer, pH 7.0 1 L 34.00 Add to Cart
  19340-72 Phosphate Buffer, pH 7.2 1 L 34.00 Add to Cart

*Meant for biological research only. Not to be used for medical or any other purposes.

For a complete line of all of our Salt Buffers, see catalog pages 23, 28-29, and 36 or click here.

msdsarrow12Phosphomolybdic Acid,

Crystal, Reagent, A.C.S.

(dodeca-Molybdophosphoric Acid)

H3PO4·12(MoO3)·24H2O   F.W. 2257.6   CAS #51429-74-4

RT 19400   100g 150.00 Add to Cart

arrow12Phosphomolybdic Acid Aqueous Solutions

RT 19402-1 Phosphomolybdic Acid 1% Aqueous 500 ml 34.00 Add to Cart
RT 19402-10 Phosphomolybdic Acid 10% Aqueous 500 ml 100.00 Add to Cart

msdsarrow12Phosphotungstic Acid, Crystal, Reagent, Highest Purity

(Tungstophosphoric Acid)

H3[P(W3O10)4]   aq F.W. 2880.17+aq   CAS #12501-23-4

Specifications:

Solubility   Practically clear, colorless solution.
Ammonia (NH )   0.004%
Chloride (Cl)   0.03%
Insoluble Matter   0.02%
Nitrate (NO3 )   0.004%
Sulfate (SO4 )   0.005%
Heavy Metals and Iron.   No color formation onaddition of Hydrogen Sulfide
Used as a Fixative:

Issidorides, M.R., and Kasorchis, T. (1981).
Dispersed and compact chromatin demonstrated with a new EM Method: phosphotungstic acid-hematoxylin block-staining. Histochemistry 73,21

Used widely as a Negative Stain:
  • As Spray method: Horne, R.W., and Pasquali-Ronchette, I. (1974). A negative staining-carbon film technique for studying viruses in the electron microscope.
  • Preparation procedures for examining icosahedral and filamentous viruses. J. Ultrastruct. Res. 47, 361. Locke, M., and Kirhman, N. (1971).
  • Hot Alcoholic phosphotungstic acid and uranyl acetate as routine stains for thick and thin sections. J. Cell Biol., 50, 550., Farragiana, T., and Marinozzi, V. (1979).
  • Phosphotungstic acid staining of polysaccharides containing structures on epoxy embedded tissues. J. Submicrosc. Cytol. 11, 263.Bloom, E.E., And Aghajanian, G.K. (1968).
  • Fine structural and cytochemical analysis of the staining of synaptic junctions with PTA. J. Ultrastruct. Res. 22, 261.
RT 19500   100g 52.00 Add to Cart

arrow12Phosphotungstic Acid Aqueous Solutions

RT 19502-1 Phosphotungstic Acid, 1% Aqueous 500 ml 32.00 Add to Cart
RT 19502-3 Phosphotungstic Acid, 3% Aqueous 500 ml 45.00 Add to Cart
RT 19502-5 Phosphotungstic Acid, 5% Aqueous 500 ml 62.00 Add to Cart

arrow12 Picric Acid, Reagent, Crystal, A.C.S.

(2,4,6-Trinitrophenol)

C6H2(NO2) 3OH   FW 229.11   CAS #88-89-1

A.C.S. Specifications:

Water   min 30%
Melting Point (dried)   121-123°C
Insoluble in Resinous Matter.   0.01%
Insoluble in benzene   0.1%
Sulfate   0.01%
RT 19550   100g 46.00 Add to Cart

arrow12Picric Acid Solutions

RT 19552 Picric Acid Saturated, Aqueous 500 ml 30.00 Add to Cart
RT 19553 Picric Acid, 0.1% in Acetone 500 ml 38.00 Add to Cart
RT 19554 Picric Acid, 4.67% in 95% Alcohol 500 ml 40.00 Add to Cart

arrow12Potassium Dichromate, Crystal, Reagent, A.C.S.

K2Cr2O7   F.W. 294.18   CAS #7778-50-9

A.C.S. Specifications:

Insoluble and NH4OH ppt. 0.005%
Chloride 0.001%
Sulfate 0.005%
Calcium 0.003%
Sodium 0.02%
Loss on drying at 105°C 0.05%
RT 20100 Potassium Dichromate 250g 21.50 Add to Cart

arrow12Potassium Dichromate Aqueous Solution

RT 20103-3 Potassium Dichromate, 3% Aqueous 500ml 27.00 Add to Cart
RT 20103-4 Potassium Dichromate, 5% Aqueous 500ml 34.00 Add to Cart

arrow12Potassium Ferricyanide, Reagent, A.C.S.

K3Fe(CN)6   F.W. 329.25   CAS #13746-66-2

A.C.S. Specifications:

Chloride 0.01%
Ferro Compounds (Fe(Cn)4) 0.05%
Insoluble Matters. 0.005%
Sulfate 0.01%
RT 20150 Potassium Ferricyanide 100g 18.00 Add to Cart
RT 25102-20 Potassium Ferricyanide, 20% Aqueous 500 ml 39.00 Add to Cart

arrow12Potassium Ferrocyanide Aqueous Solution

RT 25154-2 Potassium Ferrocyanide, 2% Aqueous 500ml 27.00 Add to Cart
RT 25154-5 Potassium Ferrocyanide, 5% Aqueous 500ml 29.00 Add to Cart
RT 25154-10 Potassium Ferrocyanide, 10% Aqueous 500ml 33.00 Add to Cart
RT 25154-20 Potassium Ferrocyanide, 20% Aqueous 500ml 37.00 Add to Cart

arrow12Potassium Hydroxide, Pellets, Reagent, ACS.

KOH   F.W. 56.11   CAS #1310-58-3

Assay   >85.0%
Water contains:   10-15%
RT 20155 Potassium Iodide 100g 10.00 Add to Cart

arrow12Potassium Hydroxide Aqueous Solutions

RT 20156-1 Potassium Hydroxide, 1% Aqueous 500 ml 23.00 Add to Cart
RT 20156-2 Potassium Hydroxide, 4% Aqueous 500 ml 24.00 Add to Cart
RT 20156-10 Potassium Hydroxide, 10% Aqueous 500 ml 30.00 Add to Cart

arrow12Potassium Iodide, Granular, Reagent, A.C.S.

KI F.W. 166.00 CAS #7681-11-0

Refer to Bismuth Carbonate for references on use.

RT 20158 Potassium Iodide 50g 18.00 Add to Cart
RT 25109-3 Potassium Iodide, 3% Aqueous 500 ml 19.00 Add to Cart

arrow12Potassium Permanganate, Reagent, A.C.S.

KMnO4   F.W. 158.03   CAS #7722-64-7

A.C.S. Specifications:

Chloride & Chlorate 0.005%
Nitrogen Compounds 0.005%
Sulfate .0.02%
Insoluble Matters 0.2%

An EM fixative and a metal stain.

J. Ultrastruct. Res., 21,424 (1968)

RT 20200 Potassium Permanganate 250g 34.00 Add to Cart

arrow12Potassium Permanganate, Aqueous Solutions

RT 20202 Potassium Permanganate 0.25% Aqueous 500 ml 21.00 Add to Cart
RT 20203 Potassium Permanganate 0.3% Aqueous 500 ml 22.00 Add to Cart
RT 20204 Potassium Permanganate 1% Aqueous 500 ml 26.00 Add to Cart

msdsarrow12Propylene Glycol, Reagent, A.C.S.

(1,2-Propanediol)
CH3CHOHCH2OH   F.W. 76.10   CAS #57-55-6

Specific Gravity   (H2O=1): 1.04
Assay   >99.5%

Specifications:

Residue after Ignition 0.005%
Chloride 1ppm
Water 0.2%
Titratable Acid 0.0005meq/g
RT 20350   450ml 27.00 Add to Cart
RT 20352   4x450ml 94.00 Add to Cart

Rosin Solution

RT 20550-05 Rosin Alcohol Stock Solution 500 ml 36.00 Add to Cart
RT 20550-15 Rosin Alcohol Working Solution 500 ml 33.00 Add to Cart

Scott’s Tap Water Substitute

Magnesium sulfate buffered with sodium bicarbonate. Readyto- use.

RT 26070-06 Scott’s Tap Water Substitute 1 L 30.00 Add to Cart
RT 26070-07 Scott’s Tap Water Substitute 4 L 95.00 Add to Cart

msdsAlcoholic Stain SolutionsSchiff’s Reagent

For use in the Periodic Acid Leucofuchsin (PAS) method as a general tissue stain.

0-5°C 26052-05 Schiff’s Reagent 500ml 36.00 Add to Cart
0-5°C 26052-06 Schiff’s Reagent 1L 62.00 Add to Cart

Silver Nitrate, Reagent, A.C.S.

AgNO3   F.W. 169.89  CAS #7761-88-8

Assay   99.9+%

A.C.S. Specifications:

Substances not precipitated by HCl-max 0.010%
Sulfate max 0.0002%
Copper max 0.0002%
Iron max 0.0002%
Lead max 0.001%
References for Silver stain:
  • Elektronemikrokopische Swift, J.A. (1968). The electron histochemistry of cystine-containing proteins in thin transverse sections of human hair. J.R. Microsc. Soc. 88,449.
  • Elektronemikrokopische Thiery, J.P. (1967). Mise en evidence des Polysaccharides sur coupes fines en microscopie eletronique. J. Microsc. (Paris) 6,987.
  • Elektronemikrokopische Rambourg, A. (1967). An improved silver methenamine technique for the detection of periodic acidreactive complex carbohydrates with the electron microscope.J.
    Histochem. Cytochem. 15, 409.
  • Elektronemikrokopische Hermandez et al., (1968)). Periodic acid-chromic acid-methenamine silver technique for glycoprotein detection in the electron microscope. J. Histochem. Cystochem. 16, 507.
  • Elektronemikrokopische Ribi,W.A. (1976). A golgi-electron microscope method for insect nervous tissue. Stain Technol. 51,13.
  • Elektronemikrokopische Braak, H., and Braak, E. (1982). A simple procedure for electron microscopy of Golgi-impregnated nerve cells. Neurosci. Lett. 32,1.
RT 21050   25g 33.00 Add to Cart
RT 21052   100g 110.00 Add to Cart

Silver Nitrate Aqueous Solutions

RT 21054-1 Silver Nitrate, 1%, Aqueous 500 ml 50.00 Add to Cart
RT 21054-2 Silver Nitrate, 2%, Aqueous 500 ml 62.00 Add to Cart
RT 21054-5 Silver Nitrate, 5%, Aqueous 500 ml 126.00 Add to Cart
RT 21054-10 Silver Nitrate, 10%, Aqueous 500 ml 216.00 Add to Cart
RT 21054-20 Silver Nitrate, 20%, Aqueous 500 ml 325.00 Add to Cart

Sodium Bicarbonate, Powder, Reagent, A.C.S.

NaHCO3   F.W. 84.01   CAS #144-55-8

Assay   97.7-100.3%

Specifications:

Insoluble Matters .0.015%
Chloride 0.003%
Phosphate 0.001%
Sulfur Compounds 0.003%
Ammonium 5ppm
Iron 0.001%
Potassium 0.05%
RT 21125   500g 21.00 Add to Cart
RT 21127 Sodium Bicarbonate, 5% Aqueous 500 ml 16.00 Add to Cart

msdsSodium Hydroxide, Solution

See Technical Tip on uses page 41 <<<<<

CAS #1310-73-2

Carbonate-free. This solution is prepared from ACS Reagent grade sodium hydroxide, and is standardized colormetrically against potassium biphthalate primary reference material.

Normal Concentration 1.98-2.02

RT 21170 Sodium Hydroxide Solution 2N 225ml 12.00 Add to Cart
RT 21170-01 Sodium Hydroxide Solution 1N 225ml 10.00 Add to Cart

Sodium Hydroxide Aqueous Solutions

RT 21172-1 Sodium Hydroxide, 1% Aqueous 500 ml 17.00 Add to Cart
RT 21172-2 Sodium Hydroxide, 2.5% Aqueous 500 ml 22.00 Add to Cart
RT 21172-3 Sodium Hydroxide, 3.1% Aqueous 500 ml 27.00 Add to