Reagents for Histology: Fixing, Dehydrating, Clearing and Staining

HISTOCHOICE™MB® *- The Molecular Biology Fixative

SAFE - Contains no Formaldehyde, Glutaraldehyde or Mercury.
NON-TOXIC – Is completely odorless and can be safely disposed
of down the drain.

HISTOCHOICE™ MB® Tissue Fixative is the first fixative designed for the molecular biologist. It is specialty formulated to preserve antigenic sites for antibody probes and nucleic acid sites for immunohistochemistry. HISTOCHOICE™MB® replaces formaldehyde based, alcohol based, Zenkers, B-5, B-3, Bouin’s and other fixatives with superior results. Tissue fixed in HISTOCHOICE™MB® exhibit vibrant staining, better nuclear and cytoplasmic detail, and will retain a crisp appearance even after long-term fixation.

Because this formula is designed specially for molecular biology applications, HISTOCHOICE™MB® fixed sections do not require pre-digestion or other recovery procedures to make important sites available.This means you spend less time preparing slides and more time doing research.

More than a fixative, HISTOCHOICE™MB® is a preservative that leaves antigens and nucleic acids in their native state, allowing binding for specific probes. Primary antibodies can often be diluted several-fold due to the increased number of preserved antigenic sites, saving cost
on every slide you process.

HISTOCHOICE™MB® fixed tissue retains a much more natural look and feel than formalin fixed tissue. It is important to remember that these tissues are not fixed by formalin, but rather preserved by HISTOCHOICE™MB®.

RT	64115-01	HISTOCHOICE™MB® Fixative	1 L	46.00	Add to Cart
RT	64115-04	HISTOCHOICE™MB® Fixative	4 L	150.00	Add to Cart
RT	64115-44	HISTOCHOICE™MB® Fixative	4x4 L	495.00	Add to Cart

* HISTOCHOICE is registered name of Amresco

Histo-Clear®/Histo-Clear II®

Histo-Clear® (Distilled essential oils –food grade) and Histo-Clear II® (A mixtureof Aliphatic Hydrocarbon and Distilled essential oils – food grade, reduced citrus odor) are excellent non-toxic clearing agents replacing Xylene.

RT	64110-01	Histo-Clear®	1 L	24.00	Add to Cart
RT	64110-04	Histo-Clear®	1 gal	100.00	Add to Cart
RT	64110-10	Histo-Clear®	5 gal	420.00	Add to Cart
RT	64111-01	Histo-Clear II®	1 L	18.00	Add to Cart
RT	64111-04	Histo-Clear II®	1 gal	68.00	Add to Cart
RT	64111-10	Histo-Clear II®	5 gal	279.00	Add to Cart

® is register name of National Diagnostics

Hollande’s Fixative

A modification of Bouin's solution. It is stable and will decalcify small bone specimens. Tissue that is fixed with Hollande's can be stained successfully with most stains, and the cupric acetate in the solution stabilizes red blood cell membranes and cosinophil and endocrine cell granules so that less lysis occurs than with Bouin's solution. Hollande's is widely used as a fixative for biopsy specimens of the gastrointestinal tract.

Thorough washing of the fixative prior to placing the specimen in a phosphate buffered formalin solution is necessary because the salts present in the solution will form an insoluble phosphate precipitate. Packaged in leak-proof histo-containers; prefilled ready-to-use.

	Catalog #	Cup Size	Filled	Pack/cs	Price
RT	64120-10	15 ml	10ml	50	76.00	Add to Cart
RT	64120-20	30 ml	20ml	50	96.00	Add to Cart
RT	64120-30	60 ml	30ml	20	44.00	Add to Cart

Bulk packaging is available:

RT	64120-01	Hollande's Fixative	1 L	41.00	Add to Cart
RT	64120-04	Hollande's Fixative	4 L	130.00	Add to Cart

Hydrochloric Acid, Diluted, (w/v)

HCl F.W. 36.46 CAS 7647-01-0

RT	16762	Hydrochloric Acid, 1% Aqueous	500 ml	17.00	Add to Cart
RT	16763	Hydrochloric Acid, 2% Aqueous	500 ml	18.00	Add to Cart
RT	16765	Hydrochloric Acid, 10% Aqueous	500 ml	27.00	Add to Cart
RT	16766	Hydrochloric Acid, 20% Aqueous	500 ml	33.00	Add to Cart

Hydrogen Peroxide 30% Solution- Stabilized, Reagent, A.C.S.

H₂O₂ F.W. 34.01 CAS #7722-84-1

Hydrogen Peroxide, 30%	16790
Hydrogen Peroxide, 3%	16793

Assay		29-32%
Maximum of Impurities:
Chloride(Cl)		0.0003%
Nitrate		0.0002%
Phosphate		0.0002%
Sulfate		0.0005%
Heavy Metal		0.0001%
Iron		0.00005%
Titratable Acid		0.0006meq/g

RT	16790		100ml	38.50	Add to Cart
RT	16792		450ml	130.00	Add to Cart
0-4ºC	16793	Hydrogen Peroxide, 3% Aqueous	500 ml	47.00	Add to Cart

Hydroquinone Aqueous Solution

0-4ºC	16795-1	Hydroquinone, 1%	500 ml	35.00	Add to Cart
0-4ºC	16795-2	Hydroquinone, 2%	500 ml	37.00	Add to Cart
0-4ºC	16795-4	Hydroquinone, 4%	500 ml	41.00	Add to Cart

Isopropyl Alcohol, Reagent, A.C.S.

(2-Propanol)
CH₃CH(OH)CH₃ F.W. 60.10 CAS #67-63-0

A.C.S. Specifications:

Color (APHA)	.	10
Density @ 25°C	.	0.781-0.783
Boiling Range (including 82.3°C)	.	1°C
Titratable Acid or Base	.	0.0001 meq/g
Residue after Evaporation	.	0.001%
Solubility in water	.	To pass test
Water	.	0.2%

RT	16930	1qt	12.00	Add to Cart
RT	16931	4x1qt	42.00	Add to Cart

McDowell Trumps Fixative

RT	18030-05	McDowell Trumps Fixative	500 ml	30.00	Add to Cart
RT	18030-10	McDowell Trumps Fixative	1 L	36.00	Add to Cart

Michel’s Buffer

RT	18041-05	Michel’s Buffer	500 ml	19.00	Add to Cart
RT	18041-10	Michel’s Buffer	1 L	25.00	Add to Cart

Michel’s Fixative, pH 7.0 – 7.4

RT	18040-05	Michel’s Fixative	500 ml	46.00	Add to Cart
RT	18040-10	Michel’s Fixative	1 L	81.00	Add to Cart
RT	18040-20	Michel’s Fixative Pre-filled	20 x10 ml	71.00	Add to Cart

Methanol, Reagent, A.C.S.

(Methyl Alcohol)
CH₃OH F.W. 32.04 CAS #67-56-1

Assay:		99.8%
Specific Gravity :		0.79

A.C.S. Specifications:

Residue after Evaporation	.	0.001%
Boiling Range (Boiling point 64.6°C)	.	2°C max
Appearance	.	Clear
Color (A.P.H.A.)	.	10 max
Acetone & Aldehydes (0.001%)	.	To pass test
Titratable Acid	.	0.0003 meq/g
Titratable Base	.	0.0002 meq/g
Water	.	0.2% max

RT	18510	1qt	15.00	Add to Cart
RT	18511	4x1qt	54.00	Add to Cart

Periodic Acid, Reagent

H₅IO₆ F.W. 227.96 CAS #10450-60-9

Assay		>99.0%
Heavy Metals		.0.005%
Insoluble Matters		0.01%
Iron		.0.003%
Other Halogen (Cl)		0.01%
Residue after Ignition		0.01%
Sulfate		0.01%

Ainsworth, S.K. et al. (1972). Alkaline bismuth reagent for high resolution ultrastructural demonstration of periodic-reactive sites. J. Histochem. Cytochem., 20:995.
Mowry, R.W. (1958). Improved procedure for the staining of acid polysaccharides by Muller’s colloidal (hydrous) ferric oxide and its combination with the Feulgen and the periodic acid Schiff
reaction. Lab. Invest., 7:566
Mowry, R.W. (1959). Effect of Periodic Acid used prior to Chromic Acid on the staining of Polysaccharides by Gomori’s methenamine silver. J. Histochem. Cytochem., 7:288
Tsuchiya, A. and Ogawa, K. (1973). Ultracytochemistry of the Periodic Acid (PA) - Phosphotungstic Acid (PTA) reaction. J. Electr. Microsc., 22:290
Hanker, J.S. et al (1964). Osmiophilic reagents: new cytochemical principles for light and electron microscopy. Science New York, 146:1039
Derenzini, M. et al (1986). An improved periodic acid-thiosemicarbazide- osmium technique to reveal glycoconjugates at the molecular level in-situ. J. Histochem. Cytochem., 34:1161

19325

25 g

30.00

Add to Cart

Periodic Acid Aqueous Solutions

19324-05	Periodic Acid 0.5% Aqueous	500 ml	24.00	Add to Cart
19324-10	Periodic Acid 1% Aqueous	500 ml	27.00	Add to Cart
19324-50	Periodic Acid 5% Aqueous	500 ml	32.00	Add to Cart

Phosphate Buffer, 0.1M*

Prepared from Sodium Phosphate Monobasic Hydrate and Sodium Phosphate Dibasic Heptahydrate. Non-Sterile. Meant for research purposes only.

19340-60	Phosphate Buffer, pH 6.0	1 L	34.00	Add to Cart
19340-65	Phosphate Buffer, pH 6.5	1 L	34.00	Add to Cart
19340-70	Phosphate Buffer, pH 7.0	1 L	34.00	Add to Cart
19340-72	Phosphate Buffer, pH 7.2	1 L	34.00	Add to Cart

*Meant for biological research only. Not to be used for medical or any other purposes.

For a complete line of all of our Salt Buffers, see catalog pages 23, 28-29, and 36 or click here.

Phosphomolybdic Acid,

Crystal, Reagent, A.C.S.

(dodeca-Molybdophosphoric Acid)

H₃PO₄·12(MoO₃)·24H₂O F.W. 2257.6 CAS #51429-74-4

19400

100g

150.00

Add to Cart

Phosphomolybdic Acid Aqueous Solutions

RT	19402-1	Phosphomolybdic Acid 1% Aqueous	500 ml	34.00	Add to Cart
RT	19402-10	Phosphomolybdic Acid 10% Aqueous	500 ml	100.00	Add to Cart

Phosphotungstic Acid, Crystal, Reagent, Highest Purity

(Tungstophosphoric Acid)

H₃[P(W₃O₁₀)₄] aq F.W. 2880.17+aq CAS #12501-23-4

Specifications:

Solubility		Practically clear, colorless solution.
Ammonia (NH )		0.004%
Chloride (Cl)		0.03%
Insoluble Matter		0.02%
Nitrate (NO₃ )		0.004%
Sulfate (SO₄ )		0.005%
Heavy Metals and Iron.		No color formation onaddition of Hydrogen Sulfide

Used as a Fixative:

Issidorides, M.R., and Kasorchis, T. (1981).
Dispersed and compact chromatin demonstrated with a new EM Method: phosphotungstic acid-hematoxylin block-staining. Histochemistry 73,21

Used widely as a Negative Stain:

As Spray method: Horne, R.W., and Pasquali-Ronchette, I. (1974). A negative staining-carbon film technique for studying viruses in the electron microscope.
Preparation procedures for examining icosahedral and filamentous viruses. J. Ultrastruct. Res. 47, 361. Locke, M., and Kirhman, N. (1971).
Hot Alcoholic phosphotungstic acid and uranyl acetate as routine stains for thick and thin sections. J. Cell Biol., 50, 550., Farragiana, T., and Marinozzi, V. (1979).
Phosphotungstic acid staining of polysaccharides containing structures on epoxy embedded tissues. J. Submicrosc. Cytol. 11, 263.Bloom, E.E., And Aghajanian, G.K. (1968).
Fine structural and cytochemical analysis of the staining of synaptic junctions with PTA. J. Ultrastruct. Res. 22, 261.

19500

100g

52.00

Add to Cart

Phosphotungstic Acid Aqueous Solutions

RT	19502-1	Phosphotungstic Acid, 1% Aqueous	500 ml	32.00	Add to Cart
RT	19502-3	Phosphotungstic Acid, 3% Aqueous	500 ml	45.00	Add to Cart
RT	19502-5	Phosphotungstic Acid, 5% Aqueous	500 ml	62.00	Add to Cart

Picric Acid, Reagent, Crystal, A.C.S.

(2,4,6-Trinitrophenol)

C₆H₂(NO₂)₃OH FW 229.11 CAS #88-89-1

A.C.S. Specifications:

Water		min 30%
Melting Point (dried)		121-123°C
Insoluble in Resinous Matter.		0.01%
Insoluble in benzene		0.1%
Sulfate		0.01%

19550

100g

46.00

Add to Cart

Picric Acid Solutions

RT	19552	Picric Acid Saturated, Aqueous	500 ml	30.00	Add to Cart
RT	19553	Picric Acid, 0.1% in Acetone	500 ml	38.00	Add to Cart
RT	19554	Picric Acid, 4.67% in 95% Alcohol	500 ml	40.00	Add to Cart

Potassium Dichromate, Crystal, Reagent, A.C.S.

K₂Cr₂O₇ F.W. 294.18 CAS #7778-50-9

A.C.S. Specifications:

Insoluble and NH₄OH ppt.	0.005%
Chloride	0.001%
Sulfate	0.005%
Calcium	0.003%
Sodium	0.02%
Loss on drying at 105°C	0.05%

20100

Potassium Dichromate

250g

21.50

Add to Cart

Potassium Dichromate Aqueous Solution

RT	20103-3	Potassium Dichromate, 3% Aqueous	500ml	27.00	Add to Cart
RT	20103-4	Potassium Dichromate, 5% Aqueous	500ml	34.00	Add to Cart

Potassium Ferricyanide, Reagent, A.C.S.

K₃Fe(CN)₆ F.W. 329.25 CAS #13746-66-2

A.C.S. Specifications:

Chloride	0.01%
Ferro Compounds (Fe(Cn)₄)	0.05%
Insoluble Matters.	0.005%
Sulfate	0.01%

RT	20150	Potassium Ferricyanide	100g	18.00	Add to Cart
RT	25102-20	Potassium Ferricyanide, 20% Aqueous	500 ml	39.00	Add to Cart

Potassium Ferrocyanide Aqueous Solution

RT	25154-2	Potassium Ferrocyanide, 2% Aqueous	500ml	27.00	Add to Cart
RT	25154-5	Potassium Ferrocyanide, 5% Aqueous	500ml	29.00	Add to Cart
RT	25154-10	Potassium Ferrocyanide, 10% Aqueous	500ml	33.00	Add to Cart
RT	25154-20	Potassium Ferrocyanide, 20% Aqueous	500ml	37.00	Add to Cart

Potassium Hydroxide, Pellets, Reagent, ACS.

KOH F.W. 56.11 CAS #1310-58-3

Assay		>85.0%
Water contains:		10-15%

20155

Potassium Iodide

100g

10.00

Add to Cart

Potassium Hydroxide Aqueous Solutions

RT	20156-1	Potassium Hydroxide, 1% Aqueous	500 ml	23.00	Add to Cart
RT	20156-2	Potassium Hydroxide, 4% Aqueous	500 ml	24.00	Add to Cart
RT	20156-10	Potassium Hydroxide, 10% Aqueous	500 ml	30.00	Add to Cart

Potassium Iodide, Granular, Reagent, A.C.S.

KI F.W. 166.00 CAS #7681-11-0

Refer to Bismuth Carbonate for references on use.

RT	20158	Potassium Iodide	50g	18.00	Add to Cart
RT	25109-3	Potassium Iodide, 3% Aqueous	500 ml	19.00	Add to Cart

Potassium Permanganate, Reagent, A.C.S.

KMnO₄ F.W. 158.03 CAS #7722-64-7

A.C.S. Specifications:

Chloride & Chlorate	0.005%
Nitrogen Compounds	0.005%
Sulfate	.0.02%
Insoluble Matters	0.2%

An EM fixative and a metal stain.

J. Ultrastruct. Res., 21,424 (1968)

20200

Potassium Permanganate

250g

34.00

Add to Cart

Potassium Permanganate, Aqueous Solutions

RT	20202	Potassium Permanganate 0.25% Aqueous	500 ml	21.00	Add to Cart
RT	20203	Potassium Permanganate 0.3% Aqueous	500 ml	22.00	Add to Cart
RT	20204	Potassium Permanganate 1% Aqueous	500 ml	26.00	Add to Cart

Propylene Glycol, Reagent, A.C.S.

(1,2-Propanediol)
CH₃CHOHCH₂OH F.W. 76.10 CAS #57-55-6

Specific Gravity		(H₂O=1): 1.04
Assay		>99.5%

Specifications:

Residue after Ignition	0.005%
Chloride	1ppm
Water	0.2%
Titratable Acid	0.0005meq/g

RT	20350		450ml	27.00	Add to Cart
RT	20352		4x450ml	94.00	Add to Cart

Rosin Solution

RT	20550-05	Rosin Alcohol Stock Solution	500 ml	36.00	Add to Cart
RT	20550-15	Rosin Alcohol Working Solution	500 ml	33.00	Add to Cart

Scott’s Tap Water Substitute

Magnesium sulfate buffered with sodium bicarbonate. Readyto- use.

RT	26070-06	Scott’s Tap Water Substitute	1 L	30.00	Add to Cart
RT	26070-07	Scott’s Tap Water Substitute	4 L	95.00	Add to Cart

Schiff’s Reagent

For use in the Periodic Acid Leucofuchsin (PAS) method as a general tissue stain.

0-5°C	26052-05	Schiff’s Reagent	500ml	36.00	Add to Cart
0-5°C	26052-06	Schiff’s Reagent	1L	62.00	Add to Cart

Silver Nitrate, Reagent, A.C.S.

AgNO₃ F.W. 169.89 CAS #7761-88-8

Assay

99.9+%

A.C.S. Specifications:

Substances not precipitated by HCl-max	0.010%
Sulfate max	0.0002%
Copper max	0.0002%
Iron max	0.0002%
Lead max	0.001%

References for Silver stain:

Elektronemikrokopische Swift, J.A. (1968). The electron histochemistry of cystine-containing proteins in thin transverse sections of human hair. J.R. Microsc. Soc. 88,449.
Elektronemikrokopische Thiery, J.P. (1967). Mise en evidence des Polysaccharides sur coupes fines en microscopie eletronique. J. Microsc. (Paris) 6,987.
Elektronemikrokopische Rambourg, A. (1967). An improved silver methenamine technique for the detection of periodic acidreactive complex carbohydrates with the electron microscope.J.
Histochem. Cytochem. 15, 409.
Elektronemikrokopische Hermandez et al., (1968)). Periodic acid-chromic acid-methenamine silver technique for glycoprotein detection in the electron microscope. J. Histochem. Cystochem. 16, 507.
Elektronemikrokopische Ribi,W.A. (1976). A golgi-electron microscope method for insect nervous tissue. Stain Technol. 51,13.
Elektronemikrokopische Braak, H., and Braak, E. (1982). A simple procedure for electron microscopy of Golgi-impregnated nerve cells. Neurosci. Lett. 32,1.

RT	21050		25g	33.00	Add to Cart
RT	21052		100g	110.00	Add to Cart

Silver Nitrate Aqueous Solutions

RT	21054-1	Silver Nitrate, 1%, Aqueous	500 ml	50.00	Add to Cart
RT	21054-2	Silver Nitrate, 2%, Aqueous	500 ml	62.00	Add to Cart
RT	21054-5	Silver Nitrate, 5%, Aqueous	500 ml	126.00	Add to Cart
RT	21054-10	Silver Nitrate, 10%, Aqueous	500 ml	216.00	Add to Cart
RT	21054-20	Silver Nitrate, 20%, Aqueous	500 ml	325.00	Add to Cart

Sodium Bicarbonate, Powder, Reagent, A.C.S.

NaHCO₃ F.W. 84.01 CAS #144-55-8

Assay

97.7-100.3%

Specifications:

Insoluble Matters	.0.015%
Chloride	0.003%
Phosphate	0.001%
Sulfur Compounds	0.003%
Ammonium	5ppm
Iron	0.001%
Potassium	0.05%

RT	21125		500g	21.00	Add to Cart
RT	21127	Sodium Bicarbonate, 5% Aqueous	500 ml	16.00	Add to Cart

Sodium Hydroxide, Solution

See Technical Tip on uses page 41 <<<<<

CAS #1310-73-2

Carbonate-free. This solution is prepared from ACS Reagent grade sodium hydroxide, and is standardized colormetrically against potassium biphthalate primary reference material.

Normal Concentration 1.98-2.02

RT	21170	Sodium Hydroxide Solution 2N	225ml	12.00	Add to Cart
RT	21170-01	Sodium Hydroxide Solution 1N	225ml	10.00	Add to Cart

Sodium Hydroxide Aqueous Solutions

RT	21172-1	Sodium Hydroxide, 1% Aqueous	500 ml	17.00	Add to Cart
RT	21172-2	Sodium Hydroxide, 2.5% Aqueous	500 ml	22.00	Add to Cart
RT	21172-3	Sodium Hydroxide, 3.1% Aqueous	500 ml	27.00	Add to

Electron Microscopy Sciences

Histology and Light Microscopy

Reagents for Histology: Fixing, Dehydrating, Clearing and Staining

HISTOCHOICE™MB® *- The Molecular Biology Fixative

Histo-Clear®/Histo-Clear II®

Hollande’s Fixative

Hydrochloric Acid, Diluted, (w/v)

Hydrogen Peroxide 30% Solution- Stabilized, Reagent, A.C.S.

Hydroquinone Aqueous Solution

Isopropyl Alcohol, Reagent, A.C.S.

McDowell Trumps Fixative

Michel’s Buffer

Michel’s Fixative, pH 7.0 – 7.4

Methanol, Reagent, A.C.S.

Periodic Acid, Reagent

Periodic Acid Aqueous Solutions

Phosphate Buffer, 0.1M*

Phosphomolybdic Acid Aqueous Solutions

Phosphotungstic Acid, Crystal, Reagent, Highest Purity

Used as a Fixative:

Used widely as a Negative Stain:

Phosphotungstic Acid Aqueous Solutions

Picric Acid, Reagent, Crystal, A.C.S.

Picric Acid Solutions

Potassium Dichromate, Crystal, Reagent, A.C.S.

Potassium Dichromate Aqueous Solution

Potassium Ferricyanide, Reagent, A.C.S.

Potassium Ferrocyanide Aqueous Solution

Potassium Hydroxide, Pellets, Reagent, ACS.

Potassium Hydroxide Aqueous Solutions

Potassium Iodide, Granular, Reagent, A.C.S.

Potassium Permanganate, Reagent, A.C.S.

Potassium Permanganate, Aqueous Solutions

Propylene Glycol, Reagent, A.C.S.

Rosin Solution

Scott’s Tap Water Substitute

Schiff’s Reagent

Silver Nitrate, Reagent, A.C.S.

References for Silver stain:

Silver Nitrate Aqueous Solutions

Sodium Bicarbonate, Powder, Reagent, A.C.S.

Sodium Hydroxide, Solution

Sodium Hydroxide Aqueous Solutions