Prepared Staining Kits for Histology and Pathology

H. FAT AND LIPID STAINS

Herxheimer’s Technique

To stain fat and cholesterol.

Stain results:

Nuclei:		Blue
Fat:		Orange-Red
Cholesterol:		Red

References:

• Clark, G., Ed.: Staining Procedures; 3rd Ed.; Baltimore: Williams and Wilkins Co., c. 1973, p. 143
• Conn’s, H.J.: Biological Stains; 8th Ed., Baltimore: Williams and Wilkins Co., c. 1969, p.100

RT	26500-01	Sudan IV Staining Solution (Herxheimer II)	500 ml	84.00	Add to Cart
RT	26500-02	Harris’ Hematoxylin	500 ml	33.00	Add to Cart
RT	26500-04	Acetic Acid, 1%, Aqueous	500 ml	17.00	Add to Cart

Fat Stains in Supersaturated Alcohol

For fat and lipid stain.

Stain results:

Fat:		Deep orange-red with Oil Red O (lighter with Sudan IV) Orange-yellow with Sudan II
Nuclei:		Blue
Erythrocytes:		Sometimes Green
Cytoplasm:		Lighter Green

References:

• Lillie, R.D.,Histopathologic Technical and Practical Histochemistry, 3rd edition, McGraw-Hill, NY, 1965
• Clark, G., (ed) Staining Procedures, 3rd edition, Williams & Wilkins, Baltimore, p. 149, c. 1973

RT	26503-01	Sudan IV-Isopropanol Solution	500 ml	84.00	Add to Cart
RT	26503-02	Oil Red O-Isopropanol Solution	250 ml	15.00	Add to Cart
RT	26503-03	Sudam III-Isopropanol Solution	250 ml	17.00	Add to Cart
RT	26503-04	Mayer Hematoxylin	500 ml	39.00	Add to Cart
RT	26503-05	Erhlich’s Hematoxylin, Diluted	500 ml	39.00	Add to Cart
RT	26503-06	Sodium Phosphate, 1%, Aqueous	500 ml	18.00	Add to Cart

Oil Red in Propylene Glycol Method

Fat Stain results:

Fat:		Red
Nuclei:		Blue

References:

AFIP Manual of Histologic Staining Methods, Ed. L. Luna, 3rd ed., New York, McGraw-Hill Publications, c. 1968, p. 140

26504-01	Oil Red O Solution	46.00	500 ml	Add to Cart
26504-02	Mayer Hematoxylin	37.00	500 ml	Add to Cart
26504-2A	Harris Hematoxylin	32.00	500 ml	Add to Cart
26504-03	Propylene Glycol, 85%, Aqueous	20.00	500 ml	Add to Cart
26504-04	Hydrochloric Acid, 5%	16.00	500 ml	Add to Cart
26504-05	Ammonia Water, 0.3%	12.00	500 ml	Add to Cart
20350	Propylene Glycol, Absolute	27.00	450 ml	Add to Cart

Technical Tip

Hematoxylin Staining Tips

Nile Blue A Stain

Lipid stain.

Stain results:

Lipofuscins:		Unstained – Natural Yellow
Melanin’s:		Green

RT	26511-01	Nile Blue A Staining Solution	500 ml	39.00	Add to Cart
RT	26511-02	Sulfuric Acid, 1% Aqueous	500 ml	19.00	Add to Cart

Pearse’s Method for Phospholipids

For the staining of phospholipids, early lipofuchsin, etc.

Stain results:

Phospholipids:		Blue
Nuclei and Nucleoli:		Red
Early lipofuchsin, eosinophil granules, keratin, keratohyalin, human elastic tissues:		Blue to Purple

References:

• Pearse, A. G.E., J. Pathl. Bact., 70:554-557, 1995
• Luna, L.G. (ed), Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology, 3rd Ed., McGraw-Hill, c. 1968, p. 148.

RT	26516-01	Luxol Fast Blue MBS Solution, 0.1%	500 ml	29.00	Add to Cart
RT	26516-02	Lithium Carbonate, 0.05% Aqueous	500 ml	22.00	Add to Cart
RT	26516-03	Neutral Red Solution, 1%, Aqueous	250 ml	20.00	Add to Cart

Mineral & Pigment Prepared Stains