I. MINERAL AND PIGMENT STAINS

Rhodanine Method for Copper

Mineral and Pigment stain.

Stain results:

Copper:		Bright Red to Red Yellow
Nuclei:		Light Blue

References:

• Sheehan and Hrapchack, Theory and Practice of Histotechnology. St. Louis, The Mosby Company 1980 p. 230.

RT	26602-01	Rhodanine Saturated Solution	100ml	45.00	Add to Cart
RT	26602-02	Mayer’s Hematoxylin	500ml	28.00	Add to Cart
RT	26602-03	Sodium Borate, 5% Aqueous	500ml	21.00	Add to Cart

Johnson’s Method for Iron - (Microincineration)

Mineral and Pigment stain.

Stain results:

Iron Oxide:

Blue

References:

• Fenton R.H., Johnson, F.B., and Zimmerman, L.E., J. Histo-chem. Cytochem., 12:153-155, 1964.
• Luna, L.G., (ed.), Manual of Histologic Staining Methods of the AFIP, 3rd edition, McGraw-Hill, NY, p.181, c 1968.

RT	26603-01	Potassium Ferrocyanide Sol, 5%	500ml	18.00	Add to Cart
RT	26603-02	Hydrochloric Acid, 5%	500ml	16.00	Add to Cart

Lillie’s Method for Iron

For the staining of ferric and ferrous iron.

Stain results:

Ferric Iron:		Dark Prussian Blue
Ferrous Iron:		Dark Turnbull’s Blue
Background:		Light Red

References:

• Lillie, R.D., Histopathologic Technic & Practical Histo-Chemistry, 3rd Ed., McGraw-Hill, N.Y., p. 407, c.1965.
• Luna, G., (ed) Manual of Histologic Staining Methods of the AFIP, 3rd Ed., McGraw-Hill, N.Y., p. 182, c. 1968.

RT	26604-01	Potassium Ferrocyanide (For Ferric Iron) 50 g	50 g	16.00	Add to Cart
RT	26604-1A	Potassium Ferricyanide (For Ferrous Iron)	50 g	9.50	Add to Cart
RT	26604-02	Hydrochloric Acid, 0.5%	250 ml	21.00	Add to Cart
RT	26604-03	Basic Fuchsin, 0.5% Solution	250 ml	25.00	Add to Cart
RT	26604-04	Acetic Acid, 1%, Aqueous	500 ml	20.00	Add to Cart

Oil Red O Method for Lipofuscin

Pigment stain.

Stain results:

Lipofuscin:		Red
Nuclei:		Blue

References:

• Luna, L.G., (ED.), manual of Histologic Staining Methods of the AFIP, 3rd Edition. The AFIP, 3rd Edition, McGraw-Hill, NY, p 186, c 1968.

RT	26609-01	Oil Red O Solution, 0.5%	500ml	42.00	Add to Cart
RT	26609-02	Propylene Glycol, 85%, Aqueous	500ml	22.00	Add to Cart
RT	26609-03	Mayer’s Hematoxylin	500ml	29.00	Add to Cart

Hall’s Method for Bilirubin

Stain results:

Biliverdin:		Green
Collagen:		Red
Muscle:		Yellow

References:

• Hall, M.J., Amer. J. Clin., 34:313 –316, 1960
• Luna, L.G., (ed), Manual of Histologic Staining Methods of the AFIP, 3rd Ed. McGraw-Hill, N.Y., p. 174, c. 1968

RT	26611-01	Fouchet’s Reagent Refrigerated!	250 ml	33.00	Add to Cart
RT	26611-02	Van Gieson Solution	500 ml	26.00	Add to Cart

Prussian Blue Method for Hemosiderin

For the staining of deposits of hemosiderin.

Stain results:

Hemosiderin:		Blue or Green
Nuclei:		Red
Background:		Pink

References:

• Perls, M., Virchow’s Arch. F. Path. Anat., 39:42-48, 1867
• Highman, B., Arch. Path., 33:937-938, 1942
• Lillie, R.D. Histopathologic Technic and Practical Histo-chemistry, 3rd Ed. McGraw-Hill, N.Y., 1965
• Clark, G., (ed), Staining Procedures 3rd Ed., Williams & Wilkins, Baltimore, p. 157, c. 1973

RT	26613-01	Potassium Ferrocyanide, 2%, Aqueous	500 ml	26.00	Add to Cart
RT	26613-02	Hydrochloric Acid, 2%	500 ml	23.00	Add to Cart
RT	26613-03	Safranin O, 0.2%	500 ml	29.00	Add to Cart
RT	26613-04	Acetic Acid, 1%, Aqueous	500 ml	20.00	Add to Cart

Gomori’s Methenamine Silver for Urate Crystals Urate crystals.

Stain results:

Urate Crystals:		Black
Background:		Green

References:

• Sheehan, Dezna C. and Hrapchak, Barbara B.: Theory and Practice of Histotechnology, 2nd Ed.
• The C.V. Mosby Company, St. Louis, p.225-226, c. 1980.

RT	26614-01	Silver Nitrate, 5%, Aqueous	250 ml	72.00	Add to Cart
RT	26614-02	Methenamine, 3%, Aqueous	500 ml	27.00	Add to Cart
RT	26614-03	Sodium Borate, 5%, Aqueous	500 ml	25.00	Add to Cart
RT	26614-04	Gold Chloride, 0.1%	250 ml	32.00	Add to Cart
RT	26614-05	Sodium Thiosulfate, 3%, Aqueous	500 ml	19.00	Add to Cart
RT	26614-06	Light Green Working Solution	500 ml	36.00	Add to Cart