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Electron Microscopy Sciences

Histology and Light Microscopy

arrow13Prepared Staining Kits for Histology and Pathology

K. NUCLEAR STAINS

Hematoxylin Method

Alcoholic Stain SolutionsarrowEhrlich’s Hematoxylin

Mallory (1938); Ehrlich (1886)

Nuclei

References:

  • Ehrlich, P., Biol. Centralbl., 6:214, 1886
  • Mallory, F.B., Pathological Technique, W.B. Saunders Co Philadelphia, p 73, 1938
  • Clark G., (ed.) Staining Procedures, 3rd edition, Williams & Wilkins, Baltimore, c. 1973, pp 33-34
  • Luna, L.G., (Ed.), Manual of Histologic Staining Methods of the AFIP, 3rd ed, McGraw-Hill, NY, pp35 and 38, c 1968
RT 26753-01 Ehrlich’s Hematoxylin Solution msds 500 ml 41.00 Add to Cart
RT 26753-02 Acid Alcohol, 1% msds 500 ml 19.00 Add to Cart
RT 26753-03 Sodium Acetate, 1% Aqueous   500 ml 17.00 Add to Cart
RT 26753-3A Ammonium Water, 0.3% msds 500 ml 17.00 Add to Cart
RT 26753-3B Lithium Carbonate, Saturated Aqueous msds 500 ml 30.00 Add to Cart

Alcoholic Stain SolutionsarrowHarris Hematoxylin (1900)

Nuclei

RT 26754-01 Harris Hematoxylin Solution msds 500 ml 30.00 Add to Cart
RT 26754-1A Harris’ Hematoxylin without Mecury msds 500 ml 34.00 Add to Cart
RT 26754-03 Acid Alcohol, 1% msds 500 ml 19.00 Add to Cart
RT 26754-04 Sodium Acetate Solution, 1% Aqueous msds 500 ml 17.00 Add to Cart
RT 26754-4A Ammonia Water, 0.3% msds 500 ml 17.00 Add to Cart
RT 26754-4B Lithium Carbonate, Saturated Aqueous msds 500 ml 30.00 Add to Cart

Alcoholic Stain SolutionsarrowWeigert’s Iron Hematoxylinwith Methachromic Dyes

For Nuclear stain.

Stain results:

Nuclei   Black
Cytoplasm   Gray-Green
Mucus, Cartilage and   Yellowish Brown, Deep Red or
Mast Cell Granules   Orange-Red depending on dye used

References:

  • Weigert, K., Z. Wissen. Mikro., 21:1-5, 1904
  • Lillie, R.D., Histopathologic Technical and Practical Histochemistry, 3rd edition, McGraw-Hill, NY, 1965
  • Clark, G., (ed.), Staining Procedures, 3rd Edition, Williams & Wilkins, Baltimore, p.40, c 1973.
RT 26758-01 Weigert's Iron Hematoxylin A msds 500 ml 37.00 Add to Cart
RT 26758-02 Weigert's Iron Hematoxylin B msds 500 ml 27.00 Add to Cart
RT 26758-03 Fast Green FCF Solution, 0.02%   500 ml 23.00 Add to Cart
RT 26758-04 Bismark BrownY Solution, 0.1%, msds 500 ml 22.00 Add to Cart
RT 26758-05 Safranin O, 0.1%   500 ml 33.00 Add to Cart
RT 26758-06 Acetic Acid, 1% Aqueous msds 500 ml 15.00 Add to Cart

Counterstains for Hematoxylin

arrowEosin Y

RT 26762-01 Eosin Y Solution, 1%, Alcoholic msds 500 ml 22.00 Add to Cart
RT 26762-1A Eosin Y Solution, 1 % Aqueous msds 500 ml 24.00 Add to Cart

arrowEosin-Phloxine Counterstain

RT 26763-01 Eosin Y Solution, 1% Aqueous msds 500 ml 24.00 Add to Cart
RT 26763-02 Phloxine B Stock Solution, 1% Aqueous   500 ml 25.00 Add to Cart
RT 26763-03 Eosin-Phloxine Solution, Alcoholic msds 500 ml 27.00 Add to Cart

 

Nuclear Stains – Others

Alcoholic Stain SolutionsarrowWolbach’s Giemsa Method

Wolbach, Todd, and Palfrey (1922)

For the staining of : nuclei, collagen, riskettsia and bacteria.

Stain Results:

Nuclei, Bacteria:   Blue
Rickettsia:   Purple
Collagen, other tissue elements:   Pink to rose

References:

  • Wolbach, S.B., Todd, J.L., and Paltrey, F.W., The Etiology of Pathology Typhus, Harvard University Press, Cambridge, MA, p. 13-14, c. 1922
  • Luna, L.G., (ed). Manual of Histologic Staining Methods od the AFIP, 3rd edition. McGraw-Hill, NY, p. 119, c. 1968
RT 26773-01 Giemsa Stock Solution   250 ml 33.00 Add to Cart
RT 26773-1A Giemsa Working Solution msds 500 ml 43.00 Add to Cart
RT 26773-02 Rosin Alcohol Stock Solution, 10%   250 ml 17.00 Add to Cart
RT 26773-2A Rosin Alcohol Working Solution   500 ml 30.00 Add to Cart
RT 26773-03 Lugol’s Iodine   500 ml 23.00 Add to Cart
RT 26773-3A Gram’s Iodine Solution msds 500 ml 25.00 Add to Cart
RT 26773-04 Sodium Thiosulfate, 5% Aqueous   500 ml 21.00 Add to Cart

Alcoholic Stain SolutionsarrowLillie’s Method for Nucleic Acids (1965)

Stain: Nuclear chromatin, and chromatin of plasmodia, etc.

Stain Results: Cells:

Nuclear chromatin:   Deep red-purple
Cytoplasm:   Light Green
The chromatin of plasmodia, sarcosporidia toxoplasmata
and histoplasmata:
  Pale Red

References:

  • Lillie, R.D., Histopathologic Technic and Practical Histochemistry, 3rd ed, McGraw-Hill, NY, pp 149-150, c. 1965
  • Luna, L.G., (ed), Manual of Histologic Staining Methods of the AFIP, 3rd ed. McGraw-Hill, NY, p. 131, c. 1968.
0-4°C 26774-01 Schiff’s Reagent   500 ml 44.00 Add to Cart
RT 26774-02 Hydrochloric Acid, 1N msds 500 ml 16.00 Add to Cart
RT 26774-03 Sodium Bisulfite Solution 0.05M, Aqueous   500 ml 20.00 Add to Cart
RT 26774-04 Fast Green FCF Solution, 0.01%   500 ml 23.00 Add to Cart

Alcoholic Stain SolutionsarrowTaft’s Method for Nucleic Acid (1951)

Stain DNA & RNA

Stain Results:

DNA, Deoxryibonucleic Acid:   Blue Green
RNA, Ribonucleic Acid:   Bright Red

References:

  • Taft, E.B., Stain Tech., 26:205-212, 1951
RT 26777-01 Methyl Green – Pyronin Solution msds 500 ml 50.00 Add to Cart
RT 26777-02 Differentiating Solution msds 500 ml 34.00 Add to Cart

Papanicolaou, General & Misc Stains arrow13arrow13