K. NUCLEAR STAINS

Hematoxylin Method

Ehrlich’s Hematoxylin

Mallory (1938); Ehrlich (1886)

Nuclei

References:

• Ehrlich, P., Biol. Centralbl., 6:214, 1886
• Mallory, F.B., Pathological Technique, W.B. Saunders Co Philadelphia, p 73, 1938
• Clark G., (ed.) Staining Procedures, 3rd edition, Williams & Wilkins, Baltimore, c. 1973, pp 33-34
• Luna, L.G., (Ed.), Manual of Histologic Staining Methods of the AFIP, 3rd ed, McGraw-Hill, NY, pp35 and 38, c 1968

RT	26753-01	Ehrlich’s Hematoxylin Solution	500 ml	59.00	Add to Cart
RT	26753-02	Acid Alcohol, 1%	500 ml	35.00	Add to Cart
RT	26753-03	Sodium Acetate, 1% Aqueous	500 ml	26.00	Add to Cart
RT	26753-3A	Ammonium Water, 0.3%	500 ml	16.00	Add to Cart
RT	26753-3B	Lithium Carbonate, Saturated Aqueous	500 ml	26.00	Add to Cart

Harris Hematoxylin (1900)

Nuclei

RT	26754-01	Harris Hematoxylin Solution	500 ml	29.00	Add to Cart
RT	26754-1A	Harris’ Hematoxylin without Mecury	500 ml	28.00	Add to Cart
RT	26754-03	Acid Alcohol, 1%	500 ml	35.00	Add to Cart
RT	26754-04	Sodium Acetate Solution, 1% Aqueous	500 ml	26.00	Add to Cart
RT	26754-4A	Ammonia Water, 0.3%	500 ml	16.00	Add to Cart
RT	26754-4B	Lithium Carbonate, Sat’d, Aqueous	500 ml	26.00	Add to Cart

Weigert’s Iron Hematoxylinwith Methachromic Dyes

For Nuclear stain.

Stain results:

Nuclei		Black
Cytoplasm		Gray-Green
Mucus, Cartilage and		Yellowish Brown, Deep Red or
Mast Cell Granules		Orange-Red depending on dye used

References:

• Weigert, K., Z. Wissen. Mikro., 21:1-5, 1904
• Lillie, R.D., Histopathologic Technical and Practical Histochemistry, 3rd edition, McGraw-Hill, NY, 1965
• Clark, G., (ed.), Staining Procedures, 3rd Edition, Williams & Wilkins, Baltimore, p.40, c 1973.

RT	26758-01	Weigert’s Iron Hematoxylin, Sol A	500ml	34.00	Add to Cart
RT	26758-02	Weigert’s Iron Hematoxylin, Sol B	500ml	26.00	Add to Cart
RT	26758-03	Fast Green FCF Solution, 0.02%	500ml	22.00	Add to Cart
RT	26758-04	Bismark BrownY Solution, 0.1%,	500ml	21.00	Add to Cart
RT	26758-05	Safranin O, 0.1%	500ml	21.00	Add to Cart
RT	26758-06	Acetic Acid, 1% Aqueous	500ml	14.00	Add to Cart

Counterstains for Hematoxylin

Eosin Y

RT	26762-01	Eosin Y Staining Solution, 1%, Alcoholic	500 ml	23.00	Add to Cart
RT	26762-1A	Eosin Y Staining Solution, 1 % Aqueous	500 ml	27.00	Add to Cart

Eosin-Phloxine Counterstain

RT	26763-01	Eosin Y Stock Solution, 1% Aqueous	500 ml	27.00	Add to Cart
RT	26763-02	Phloxine B Stock Solution, 1% Aqueous	500 ml	29.00	Add to Cart
RT	*26763-03	Eosin-Phloxine Solution, Alcoholic	500 ml	35.00	Add to Cart

Nuclear Stains – Others

Wolbach’s Giemsa Method

Wolbach, Todd, and Palfrey (1922)

For the staining of : nuclei, collagen, riskettsia and bacteria.

Stain Results:

Nuclei, Bacteria:		Blue
Rickettsia:		Purple
Collagen, other tissue elements:		Pink to rose

References:

• Wolbach, S.B., Todd, J.L., and Paltrey, F.W., The Etiology of Pathology Typhus, Harvard University Press, Cambridge, MA, p. 13-14, c. 1922
• Luna, L.G., (ed). Manual of Histologic Staining Methods od the AFIP, 3rd edition. McGraw-Hill, NY, p. 119, c. 1968

RT	26773-01	Giemsa Stock Solution	250 ml	35.00	Add to Cart
RT	26773-1A	Giemsa Working Solution	500 ml	33.00	Add to Cart
RT	26773-02	Rosin Alcohol Stock Solution, 10%	250 ml	19.00	Add to Cart
RT	26773-2A	Rosin Alcohol Working Solution	500 ml	26.00	Add to Cart
RT	26773-03	Lugol’s Iodine	500 ml	24.00	Add to Cart
RT	26773-3A	Gram’s Iodine	500 ml	25.00	Add to Cart
RT	26773-04	Sodium Thiosulfate, 5% Aqueous	500 ml	16.00	Add to Cart

Lillie’s Method for Nucleic Acids (1965)

Stain: Nuclear chromatin, and chromatin of plasmodia, etc.

Stain Results: Cells:

Nuclear chromatin:		Deep red-purple
Cytoplasm:		Light Green
The chromatin of plasmodia, sarcosporidia toxoplasmata and histoplasmata:		Pale Red

References:

• Lillie, R.D., Histopathologic Technic and Practical Histochemistry, 3rd ed, McGraw-Hill, NY, pp 149-150, c. 1965
• Luna, L.G., (ed), Manual of Histologic Staining Methods of the AFIP, 3rd ed. McGraw-Hill, NY, p. 131, c. 1968.

0-4°C	26774-01	Schiff’s Reagent	500 ml	33.00	Add to Cart
RT	26774-02	Hydrochloric Acid, 1N	500 ml	15.00	Add to Cart
RT	26774-03	Sodium Bisulfite Solution 0.05M, Aqueous	500 ml	19.00	Add to Cart
RT	26774-04	Fast Green FCF Solution, 0.01%	500 ml	33.00	Add to Cart

Taft’s Method for Nucleic Acid (1951)

Stain DNA & RNA

Stain Results:

DNA, Deoxryibonucleic Acid:		Blue Green
RNA, Ribonucleic Acid:		Bright Red

References:

• Taft, E.B., Stain Tech., 26:205-212, 1951

RT	26777-01	Methyl Green – Pyronin Solution	500 ml	49.00	Add to Cart
RT	26777-02	Differentiating Solution	500 ml	33.00	Add to Cart