L. PAPANICOLAOU STAINS

Papanicolaou Stain for Membrane Filters

Stain results:

Nuclei

Deep Blue to Purple

References:

• Gill, G.W., ActaCytol., 18:300-311, 1974
• Diagnostic Cytology by Membrane Filter, Application Bulletin 100, Gelman Instrument Co., Ann Arbor, Michigan, 1978

RT	26801-01	Gill’s Hematoxylin No 1	500ml	29.00	Add to Cart
RT	26801-02	Hydrochloric Acid, 0.05%	500ml	14.00	Add to Cart
RT	26801-03	Scott’s Water Solution	500ml	16.00	Add to Cart
RT	26801-04	Modified Orange G Staining Sol.	500ml	23.00	Add to Cart
RT	26801-05	Modified EA Staining Solution	500ml	30.00	Add to Cart

M. GENERAL AND MISCELLANEOUS STAINS

Periodic Acid Leucofuchsin Method – (PAS)

For General tissue stain.

Stain results:

Nuclei		Black on Blue
Cytoplasm		Gray, Yellow or Orange (depending on the counter stain)
Collagen		Pink (Orange if Picric Acid is used as counterstain)
Reticulum		Purplish Red (Orange red if Picric acid is used)
Glycogen-		Dark Purplish Red
EpitheFilbrin- lial mucin		Red-Purple to Violet
Fibrin-		Pink (Pink to violet if the Weigert Fibrin variant was used as a counterstain)

References:

• Clark, G.: Staining Procedures, Williams & Wilkins Co., Baltimore, 3rd Ed., c. 1973, p.156.

RT	26853-01	Schiff’s Reagent	500ml	39.00	Add to Cart
RT	26853-02	Periodic Acid, 1%, Aqueous	500ml	18.00	Add to Cart
RT	26853-03	Sodium Bisulfite, 0.05M, Aque	500ml	18.00	Add to Cart
RT	26853-04	Mayer’s acid Hemalum Sol., or	500ml	38.00	Add to Cart
RT	26853-05	Weigert’s Iron Hematoxylin A	500ml	34.00	Add to Cart
RT	26853-06	Weigert’s Iron Hematoxylin B	500ml	26.00	Add to Cart
RT	26853-07	Picric Acid, Saturated, Aque,	500ml	21.00	Add to Cart
RT	26853-08	Orange G Solution, 1%	500ml	27.00	Add to Cart

Relative Aciddophilia Stain

Stain results: The least acidophilic elements stained in the phloxine
control slide lose the red color after 2 minutes in the tartrazine solution.
The most acidophilic retain the phloxine after 20 minutes in the
tartrazine.

References:

• Lendrum, A.C, J. Path. Bact., 59:399-404, 1947
• Clark, G., (ed.), Staining Procedures, 3rd Edition, Williams & Wilkins, Baltimore, p. 163, c. 1973.

RT	26901-01	Phloxine B Staining Solution	500ml	43.00	Add to Cart
RT	26901-02	Tartrazine Saturated Sol. In Cellosolve	500ml	61.00	Add to Cart
RT	26901-03	n-Butyl Alcohol	450ml	21.00	Add to Cart

Leukocyte Alkaline Phosphatase

Phosphatase stain.

References:

• Williams, W.J., Beutler, E., Erslev, A.J. and Rundles, R.W., Hematology, 2nd ed., McGraw-Hill, NY, C. 1977, p 1628-1629
• Kaplow, L.S., AM. J. Clin. Pathol., 39:439, 1693
• Kaplow, L.S. and Burstone, M.S. Nature, 200:690, 1963

RT	26913-01	Buffered Acetone Fixative	500ml	26.00	Add to Cart
RT	26913-02	Naphthol AS-BI Phosphate	100mg	42.00	Add to Cart
RT	26913-03	Dimethyl Formamide	25ml	12.00	Add to Cart
RT	26913-04	Fast Red Violet Salt LB, or	100mg	12.00	Add to Cart
RT	26913-05	Fast Violet B Salts	100mg	7.00	Add to Cart
RT	26913-06	Propanediol Buffer	100ml	7.00	Add to Cart
RT	26913-07	Hematoxylin Counter Stain	500ml	52.00	Add to Cart

AB/PAS/CG Stain

Adenohypophyseal stain.

Stain results:

ACTH/MSH Cells		Purple, Red and Orange- Red
TSH Cells		Light to dark Purple-Blue
Gonadotropin Cells		Intense Red, Blue-purple, Blue-turquoise
GH Cells		Orange

References:

• McManus, J.F.A. and Mowry, R.W., Staining Methods, Histologic and Histochemical, P.B. Hoeber, NY, 1960
• Clark, G., (ED.), staining Procedures, 3rd Edition, Williams & Wilkins, Baltimore, p. 166, c 1973.

RT	26920-01	Alcian Blue 8GX Solution 1%, pH 2.6-3.0	500ml	115.00	Add to Cart
RT	26920-02	Sodium Carbonate, 0.3% Aque	500ml	15.00	Add to Cart
RT	26920-03	Periodic Acid, 1% Aqueous	500ml	18.00	Add to Cart
RT	26920-04	Schiff’s Reagent	500ml	39.00	Add to Cart
RT	26920-05	Phophomolybdic Acid/ Orange G Sol.	500ml	45.00	Add to Cart