L. PAPANICOLAOU STAINS

Papanicolaou Stain for Membrane Filters

Stain results:

Nuclei

Deep Blue to Purple

References:

• Gill, G.W., ActaCytol., 18:300-311, 1974
• Diagnostic Cytology by Membrane Filter, Application Bulletin 100, Gelman Instrument Co., Ann Arbor, Michigan, 1978

RT	26801-01	Gill’s Hematoxylin No 1	500 ml	31.00	Add to Cart
RT	26801-02	Hydrochloric Acid, 0.05%	500 ml	15.00	Add to Cart
RT	26801-03	Scott’s Water Solution	500 ml	17.00	Add to Cart
RT	26801-04	Modified Orange G Staining Sol.	500 ml	23.00	Add to Cart
RT	26801-05	Modified EA Staining Solution	500 ml	31.00	Add to Cart

M. GENERAL AND MISCELLANEOUS STAINS

Periodic Acid Leucofuchsin Method – (PAS)

For General tissue stain.

Stain results:

Nuclei		Black on Blue
Cytoplasm		Gray, Yellow or Orange (depending on the counter stain)
Collagen		Pink (Orange if Picric Acid is used as counterstain)
Reticulum		Purplish Red (Orange red if Picric acid is used)
Glycogen-		Dark Purplish Red
EpitheFilbrin- lial mucin		Red-Purple to Violet
Fibrin-		Pink (Pink to violet if the Weigert Fibrin variant was used as a counterstain)

References:

• Clark, G.: Staining Procedures, Williams & Wilkins Co., Baltimore, 3rd Ed., c. 1973, p.156.

RT	26853-01	Schiff’s Reagent	500 ml	44.00	Add to Cart
RT	26853-02	Periodic Acid, 1%, Aqueous	500 ml	23.00	Add to Cart
RT	26853-03	Sodium Bisulfite, 0.05M, Aque	500 ml	18.00	Add to Cart
RT	26853-04	Mayer’s acid Hemalum Sol., or	500 ml	40.00	Add to Cart
RT	26853-05	Weigert’s Iron Hematoxylin A	500 ml	39.00	Add to Cart
RT	26853-06	Weigert’s Iron Hematoxylin B	500 ml	26.00	Add to Cart
RT	26853-07	Picric Acid, Saturated, Aque,	500 ml	25.00	Add to Cart
RT	26853-08	Orange G Solution, 1%	500 ml	27.00	Add to Cart

Relative Aciddophilia Stain

Stain results: The least acidophilic elements stained in the phloxine
control slide lose the red color after 2 minutes in the tartrazine solution.
The most acidophilic retain the phloxine after 20 minutes in the
tartrazine.

References:

• Lendrum, A.C, J. Path. Bact., 59:399-404, 1947
• Clark, G., (ed.), Staining Procedures, 3rd Edition, Williams & Wilkins, Baltimore, p. 163, c. 1973.

RT	26901-01	Phloxine B Staining Solution	500 ml	43.00	Add to Cart
RT	26901-02	Tartrazine Saturated Sol. In Cellosolve	500 ml	61.00	Add to Cart
RT	26901-03	n-Butyl Alcohol	450 ml	21.00	Add to Cart

Leukocyte Alkaline Phosphatase

Phosphatase stain.

References:

• Williams, W.J., Beutler, E., Erslev, A.J. and Rundles, R.W., Hematology, 2nd ed., McGraw-Hill, NY, C. 1977, p 1628-1629
• Kaplow, L.S., AM. J. Clin. Pathol., 39:439, 1693
• Kaplow, L.S. and Burstone, M.S. Nature, 200:690, 1963

RT	26913-01	Buffered Acetone Fixative	500 ml	26.00	Add to Cart
RT	26913-02	Naphthol AS-BI Phosphate	100mg	42.00	Add to Cart
RT	26913-03	Dimethyl Formamide	25 ml	12.00	Add to Cart
RT	26913-04	Fast Red Violet Salt LB, or	100mg	12.00	Add to Cart
RT	26913-05	Fast Violet B Salts	100mg	7.00	Add to Cart
RT	26913-06	Propanediol Buffer	100 ml	7.00	Add to Cart
RT	26913-07	Hematoxylin Counter Stain	500 ml	52.00	Add to Cart

AB/PAS/CG Stain

Adenohypophyseal stain.

Stain results:

ACTH/MSH Cells		Purple, Red and Orange- Red
TSH Cells		Light to dark Purple-Blue
Gonadotropin Cells		Intense Red, Blue-purple, Blue-turquoise
GH Cells		Orange

References:

• McManus, J.F.A. and Mowry, R.W., Staining Methods, Histologic and Histochemical, P.B. Hoeber, NY, 1960
• Clark, G., (ED.), staining Procedures, 3rd Edition, Williams & Wilkins, Baltimore, p. 166, c 1973.

RT	26920-01	Alcian Blue 8GX Solution 1%, pH 2.6-3.0	500 ml	78.00	Add to Cart
RT	26920-02	Sodium Carbonate, 0.3% Aque	500 ml	16.00	Add to Cart
RT	26920-03	Periodic Acid, 1% Aqueous	500 ml	24.00	Add to Cart
RT	26920-04	Schiff’s Reagent	500 ml	43.00	Add to Cart
RT	26920-05	Phophomolybdic Acid/ Orange G Sol.	500 ml	49.00	Add to Cart

Technical Tip

Achieving Maximum Contrast with Stain
Elimination of Section Contamination During Staining
Stability of Special Staining Solutions

Embedding Media