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Electron Microscopy Sciences

Specimen Preparation and Embedding Supplies

arrow14Plastic and Glass Microscope Slides

Including Plastic, Glass, Quartz, Permanox™, Polystyrene, "PTFE" Coated, Poly-L-Lysine, and Silane Treated Slides

Microscope Glass Slides - Escoarrow12Microscope Glass Slides - Esco

Our microscope glass slides are manufactured by Esco - Erie Scientific Co, where reliability and quality are recognized to be the best.

  • Made from the highest quality glass sheets.
  • The dimensions of the slides have been standardized to millimeters according to the U.S. government Specifications.
  • They are packed with care to assure cleanliness.
  • The glass is fully annealed to minimize chipping.

Our microscope glass slides are precleaned and are made from clear-white glass. They are individually inspected and free from moisture, dirt, and film. All of our slides are flat, uniform and durable. They are packed 72 slides/box and cellophane wrapped.

Chemical Makeup

  Green (%) White (%)
Silicon Dioxide 72.2 72.15
Sodium Oxide 14.5 14.25
Calcium Oxide 6.5 6.25
Magnesium Oxide 4.4 4.1
Aluminum Oxide 1.5 1.12
Potassium Oxide 0.3 1.15
Sulfur Trioxide 0.3 0.3
Iron Oxide 0.1 0.3
Titanium Oxide 0.05 0.05

Physical Properties

Glass Density: 2.4024g/cm3
Softening Point: 724°C
Annealing Point: 545°C
Strain Point: 511°C
Dispersion Value (Nu Value): 64
Dimensional Variance: ±1.5000
Thermal Expansion: (0 -300°C) 8.36x106/ °C
Dielectric Constant: @20°C (68°F) (1MHz):ET =6.7
Young’s Modulus: E = 10,000,000 lbs/sq in.
Torsion Modulus: G = 4,000,000 lbs/sq in.
Poisson’s Ratio: µ = 0.2
Luminous Transmittance: @ 0.040: 91.8%

arrow121. Microscope Slide, Plain End

Catalog # Thickness Size,mm Pack Price  
71880-01 1.2 mm 25x75 Gross 37.50 Add to Cart
71880-10 1.2 mm 25x75 10 Gross 348.00 Add to Cart
71880-20 1.2 mm 25x75 20 Gross 670.00 Add to Cart

arrow122. Microscope Slide, Frosted End

These slides have 3/4" sandblasted frosting on one end which allows for easy marking.

Catalog # Thickness Size,mm Pack Price  
71870-01 1.2 mm 25x75 Gross 43.00 Add to Cart
71870-10 1.2 mm 25x75 10 Gross 405.00 Add to Cart
71870-20 1.2 mm 25x75 20 Gross 730.00 Add to Cart

Microscope Slide, SUPERFROST® Endarrow123. Microscope Slide, SUPERFROST® End

With a superfrosted end. These slides have one side which is frosted with a brilliant white opaque coating that will take and retain all pen markings. They offer superior readability and they are permanent.

Catalog # Thickness Size,mm Pack Price  
71867-01 1 mm 25x75 Gross 37.75 Add to Cart
71867-10 1 mm 25x75 10 Gross 342.00 Add to Cart
71868-01 1.2 mm 25x75 Gross 47.00 Add to Cart
71868-10 1.2 mm 25x75 10 Gross 420.00 Add to Cart

Microscope Slide, SUPERFROST® PLUS - Adhesion Slidearrow124. SUPERFROST® PLUS - Adhesion Slide

These Superfrost Plus(+) glass slides are made by a new process which offers the following features:

  • They place a permanent positive charge in the microscope slide.
  • They electrostatically attract frozen tissue sections and cytology preparations, binding them to the slide.
  • They form a bridge so that covalent bonds develop between formalin fixed sections and the glass.
  • Tissue sections and cytological preparations adhere better to the Plus glass slides without the need for special adhesives or protein coatings.

Benefits:

  • Plus glass puts an end to background staining.
  • It eliminates blue or red background staining which is often encountered in hematoxylin and eosin staining of tissue sections mounted on albuminized glass.
  • No brown background staining occurs when immuno-peroxidase or in situ DNA procedures are performed on our Plus glass. A higher degree of enzyme digestion can be performed on plus glass without the loss of tissue sections.
  • Cytological preparations placed on Plus glass resist cell loss during enzyme digestion, DNA denaturation and RNA hybridization.
  • Plus glass improves tissue adherence in microwave staining.
  • Chromic acid washes are redundant for molecular hybridization because the slides are RNAse-free.
  • Plus slides are Color coded to help keep track of specimens during processing as well as filing.

The slides measure: 25x75 mm, 1 mm thick.

71869-10 Superfrost Plus Slide, White 1 Gross 75.00 Add to Cart
71869-11 Superfrost Plus Slide, White 10 Gross 705.00 Add to Cart
71869-20 Superfrost Plus Slide, Blue 1 Gross 75.00 Add to Cart
71869-21 Superfrost Plus Slide, Blue 10 Gross 705.00 Add to Cart
71869-30 Superfrost Plus Slide, Green 1 Gross 75.00 Add to Cart
71869-31 Superfrost Plus Slide, Green 10 Gross 705.00 Add to Cart
71869-40 Superfrost Plus Slide, Pink 1 Gross 75.00 Add to Cart
71869-41 Superfrost Plus Slide, Pink 10 Gross 705.00 Add to Cart
71869-50 Superfrost Plus Slide, Yellow 1 Gross 75.00 Add to Cart
71869-51 Superfrost Plus Slide, Yellow 10 Gross 705.00 Add to Cart

Superfrost® Plus Gold Slidesarrow125. SUPERFROST® PLUS Gold Slides

These slides are specially designed for the application of fresh or formalin fixed frozen tissue. They are inherently tissue binding so there is no need to add sprays or adhesives to the slides.

Features:

  • Securely attach frozen breast, brain, skin and other normally difficult to bond tissue sections to the slide.
  • Ideal when special stains, immunocytochemical and in situ DNA hybridization techniques are applied on fresh and fixed frozen tissue
  • Assist tissue adherence during immunocytochemical analysis of estrogen and progesterone receptors in fresh frozen breast tissue.
  • They are compatible with both toluidine blue and hematoxylin and eosin rapid frozen section stains.
71864-01 Superfrost Plus Gold Slide 25/bx 37.00 Add to Cart
71864-10 Superfrost Plus Gold Slide 200/cs 342.00 Add to Cart

Colormark™ Plus Glass Slidearrow126. Colormark™ Plus Glass Slide

Specimen Slide identification is VITAL. Automated etched slide labeling instruments ensure specimen slide integrity but are difficult to read on ordinary slides. To clearly read diamond scribed slide labels against any surface, you need the only slide designed to be used with your automated slide labeling instrument. You need COLORMARK slides.These brightly printed slides greatly improve etched labeling visibility and offer all the advantages of Superfrost¨ slides. A bright contrasting color has been printed on the back of the Superfrost end so that patient or specimen information etched through the painted end on the front is clearly visible. Colormark Plus slides are adhesive coated therefore ensuring the adhesion of the section to the slide during staining procedures.

63400-10 White Frosted End Front, Black frosted End Back 1 gross 79.00 Add to Cart
63400-11 White Frosted End Front, Black frosted End Back 10 gross 738.00 Add to Cart
63400-20 Blue Frosted End Front, Blue Frosted End Back 1 gross 82.00 Add to Cart
63400-21 Blue Frosted End Front, Blue Frosted End Back 10 gross 775.00 Add to Cart
63400-30 Green Frosted End Front, Green Frosted End Back 1 gross 82.00 Add to Cart
63400-31 Green Frosted End Front, Green Frosted End Back 10 gross 775.00 Add to Cart
63400-40 Pink Frosted End Front, Red Frosted End Back 1 gross 82.00 Add to Cart
63400-41 Pink Frosted End Front, Red Frosted End Back 10 gross 775.00 Add to Cart
63400-50 Lavender Frosted End Front, Red Frosted End Back 1 gross 82.00 Add to Cart
63400-51 Lavender Frosted End Front, Red Frosted End Back 10 gross 775.00 Add to Cart
63400-60 Lavender Frosted End Front, Black Frosted End Back 1 gross 82.00 Add to Cart
63400-61 Lavender Frosted End Front, Black Frosted End Back 10 gross 775.00 Add to Cart

arrow127. Control Slides

Use our Histology Control Slides and you will always know for sure which specimen is the Patients and which is the Control.

Features:

  • Patient and Control tissue samples on the same slide.
  • Positive identification of control and patient samples.
  • Proof of correct staining procedures.
  • Utmost adhesion of samples to slide throughout staining procedure.
  • Easy and permanent identification of slides.

Available in two configurations: Style #329+ with ¾ inch of frosted area; style 334+ with 1 inch frosted area.

63448-10 Control Slide 329+ 144/pk 67.00 Add to Cart
63448-20 Control Slide 334+ 144/pk 74.00 Add to Cart

Cytology Slidearrow128.Special Glass Slides

arrow10A. Cytology Slide: 1x3" (25x75mm), 1mm Thick

Superfrost letters VCE and lines on the same side as the Superfrost end.

71874-01 Cyto-Slide Letters VCE & Lines 1 gross 39.00 Add to Cart
71874-10 Cyto-Slide Letters VCE & Lines 10 gross 369.00 Add to Cart

Superfrost letters VC and lines on the same side as the Superfrost end.

71875-01 Cyto-Slide Letters VC & Lines 1 gross 39.00 Add to Cart
71875-10 Cyto-Slide Letters VC & Lines 10 gross 369.00 Add to Cart
Dakin - A fully frosted sandblasted surface on the same side as the Superfrosted end.
71876-01 Dakin - Fully Frosted Slide 1 gross 47.00 Add to Cart
71876-10 Dakin - Fully Frosted Slide 10 gross 410.00 Add to Cart
arrow10B. Round Corner Microslides:

This item is discontinued. Please see Superfrost Clipped Corner Slide below. 71882-01 and 71882-10.

Concavity Slidesarrow10 C. Concavity Slides

Polished spherical depression. Useful for tissue cultures.

Measurements:spacer.gif (48 bytes)

Glass Slide Size: 26mm x 76mm
Concavity Diameter: 15 - 18 mm
Depth of Well: 0.5 - 0.8 mm
Catalog# Thickness No. Wells Pack Price  
71878-01 1.4-1.6mm 1 12 /pk 52.00 Add to Cart
71878-03 1.4-1.6mm 2 12/pk 68.00 Add to Cart
71878-04 1.4-1.6mm 3 12 /pk 105.00 Add to Cart
71878-05 1.95-2.35mm 1 12 /pk 132.00 Add to Cart
71878-06 1.95-2.35mm 2 12 /pk 232.00 Add to Cart
71878-07 3.12-3.22mm 1 12 /pk 131.00 Add to Cart
71878-08 3.12-3.22mm 2 12 /pk 142.00 Add to Cart
Fluorescent Antibody Slides with Etched Ringsarrow10D. Slides with Etched Rings

Useful for Fluorescent Antibody Examination. The size of the slide is 3"x1", approximately 1 mm thick, with an etched frosted end. Pre-cleaned. Packed 72/box

71879-10 Two Etched 10mm Circle Slide Gross 37.00 Add to Cart
71879-11 Two Etched 10mm Circle Slide 10 Gross 350.00 Add to Cart
71879-15 Two Etched 15mm Circle Slide Gross 37.00 Add to Cart
71879-16 Two Etched 15mm Circle Slide 10 Gross 350.00 Add to Cart

uperFrost™ Clipped Corner Slidearrow129. SuperFrost™ Clipped Corner Slide

Designed specially for the Sysmex SP-100 slide prep unit and recommended for the Diagnostics. The 45 degree clipped corner slide offers exceptional cleanliness and resistance to sticking. The tight tolerances for the length and width help to reduce accumulation of glass chips within the instrument. The pre-cleaned slide is 25 x 75mm and approximately 1 mm thick. Ideal for technicians, who routinely prepare large number of slides.

71882-01 SuperFrost™Clipped Corner Slide 1 gross 45.00 Add to Cart
71882-10 SuperFrost™Clipped Corner Slide 10 gross 420.00 Add to Cart

Large Glass Slidesarrow12Large Glass Slides

In addition to our comprehensive collection of glass slides manufactured by ESCO we now carry large glass slides which are available in 75x38mm and 75x51mm. These slides are precleaned, individually selected and are suitable for large specimen sections, such as, brain tissue.

71860-01 75x38mm Glass Slide, 1mm Thick Gross 55.00 Add to Cart
71862-01 75x51mm Glass Slide, 1.2mm Thick Gross 75.00 Add to Cart

Microscope Slide – Gold Seal arrow12Microscope Slide – Gold Seal®

In our line of slides we proudly introduce GOLD SEAL® microscope slides for your convenience.

GOLDSEAL® slides are made from pure white glass (sodalime) and at accurately cut sizes. To preserve the highest quality from manufacturer to ultimate user, all GOLD SEAL® slides are hermetically sealed in our Moisture-Barrier™ system, providing a dry-slide environments during shipment and storage.

UltraStick™ (adhesion coating: APTES, 3-Aminopropyl Triethoxysilane)– Adhesion coated micro slides- one of the best from GOLD SEAL® are ideal for histological and cytological applications demanding superior specimen-to-slide adhesion. The enhanced silane coating eliminates tedious procedures and special additives to your water bath.

Procedures such as immunoperoxidase, in situ DNA hybridization, enzyme digestion and frozen tissue processing will benefit from the amplified adhesion characteristics of this product. Overall cost savings and assured quality of your prepared specimen will be recognized with UltraStick™ from Gold Seal®.

arrow10Plain Slides
Catalog # Gold Seal Description Pack Price  
63710-05 3010 3 x 1", 1 mm Thick 1 gross 43.00 Add to Cart
63710-25 3010 3 x 1", 1 mm Thick 25 gross 830.00 Add to Cart
63711-05 3048 3 x 1", 1.2 mm Thick 1 gross 45.00 Add to Cart
63711-25 3048 3 x 1", 1.2 mm Thick 25 gross 860.00 Add to Cart
63712-05 3010-P 3 x 1", 1mm Thick – Cytologentics processing 1 gross 43.00 Add to Cart
63712-25 3010-P 3 x 1", 1mm Thick – Cytologentics processing 25 gross 850.00 Add to Cart
arrow10Patented Moistur-Barrier™ enclosing 5 gross inner packages
Catalog # Gold Seal Description Pack Price  
63718-05 3054 25 x 75mm, 1mm Thick, Plain 5 gross 230.00 Add to Cart
63718-25 3054 3 x 1", 1 25 x 75mm, 1mm Thick, Plain Thick 25 gross 860.00 Add to Cart
63719-05 3055 25 x 75mm, 1mm Thick, White Rite-On™ Frost 5 gross 310.00 Add to Cart
63719-25 3055 25 x 75mm, 1mm Thick, White Rite-On™ Frost 25 gross 1,215.00 Add to Cart
arrow10Rite-On™ Frosted Slides with brilliant white epoxy ink
Catalog # Gold Seal Description Pack Price  
63720-05 3051 3 x 1", 1mm Thick, Frosted One End, One Side 1 gross 59.00 Add to Cart
63720-25 3051 3 x 1", 1mm Thick, Frosted One End, One Side 25 gross 1,090.00 Add to Cart
63721-05 3050 25 x 75mm, 1mm Thick, Frosted One End, One Side 1 gross 55.00 Add to Cart
63721-25 3050 25 x 75mm, 1mm Thick, Frosted One End, One Side 25 gross 1,075.00 Add to Cart
63722-05 3050-P 25 x 75mm, 1mm Thick, Frosted End, One Side, Cytogentics Processing 1 gross 55.00 Add to Cart
63722-25 3050-P 25 x 75mm, 1mm Thick, Frosted End, One Side, Cytogentics Processing 25 gross 1,075.00 Add to Cart
63723-05 3049 3 x 1", 1.2mm Thick, Frosted One End, One Side 1 gross 63.00 Add to Cart
63723-25 3049 3 x 1", 1.2mm Thick, Frosted One End, One Side 25 gross 1,350.00 Add to Cart
arrow10UltraFrost™ Frosted Slides, white epoxy ink frosted one end, one side
Catalog # Gold Seal Description Pack Price  
63728-05 3063 25 x 75mm, 1mm Thick, Frosted one End with Gold Seal Logo 1 gross 57.00 Add to Cart
63728-25 3063 25 x 75mm, 1mm Thick, Frosted one End with Gold Seal Logo 25 gross 1,090.00 Add to Cart
63729-05 3064 25 x 75mm, 1mm Thick, Frosted one End with Pre-Print Patient Information Label and Gold Seal® Logo 1 gross 60.00 Add to Cart
63729-25 3064 25 x 75mm, 1mm Thick, Frosted one End with Pre-Print Patient Information Label and Gold Seal® Logo 25 gross 1,245.00 Add to Cart
arrow10Fluorescent Antibody Slides
Catalog # Gold Seal Description Pack Price  
63732-05 3032 3 x 1", 1.2mm Thick with Two 10mm diameter circles, Frosted one Side, one End with White Epoxy Ink, including Circles 1 gross 83.00 Add to Cart
63732-02 3032 3 x 1", 1.2mm Thick with Two 10mm diameter circles, Frosted one Side, one End with White Epoxy Ink, including Circles 25 gross 1,720.00 Add to Cart
arrow10UltraStick™/UltraFrost™ Adhesion Slides frosted one end, one side with epoxy ink
Catalog # Gold Seal Description Pack Price  
63734-01 3039 25 x 75mm, 1mm Thick, Surface Coated with APTES for Adhesion 1 gross 70.00 Add to Cart
63734-10 3039 25 x 75mm, 1mm Thick, Surface Coated with APTES for Adhesion 10 gross 636.00 Add to Cart

Quartz Microscope Slides and Coverslipstechnical dataarrow12Quartz Microscope Slides and Coverslips

There are many applications that require slides and coverslips that have absolute UV transparency, and high temperature properties. EMS now is introducing the highest quality, clear fused quartz microscope slides and coverslips to meet all of these requirements.

  • The window of highest transparency to UV is between 0.15µ to 5µ.
  • Typical flatness measured 2 to 4” bands per inch.
  • Soften point is about 1250°C (2282°F).
  • Standard microscope slide thickness is 1 mm.
  • Standard coverslip thickness is 0.2 mm.
arrow10Quartz Microscope Slides
72250-01 Slide, 1” x 1” (25 x 25 mm) each 13.00 Add to Cart
72250-02 Slide, 2” x 1” (50 x 25 mm) each 21.00 Add to Cart
72250-03 Slide, 3” x 1” (75 x 25 mm) each 24.00 Add to Cart
arrow10Quartz Coverslips
72255-02 Round 1" (25mm), dia. each 22.00 Add to Cart
72256-02 Square 1" x 1" (25 x 25mm) each 22.00 Add to Cart

Permanox™ and Polystyrene Microscope Slidesarrow12 Permanox™ and Polystyrene Microscope Slides

Permanox™ is a strong, biologically inert material resistant to non-chlorinated hydrocarbons, acetone, and other EM reagents. Permanox™slides allow you to do cell fixation and staining on the slide; they can be cut with scissors. Permanox™ has not been shown to be autofluorescent. All of the microscope slides are 1" x 3".

NOTE: Long exposure to solvents will cause the slide to warp. Xylene and Toluene based mounting media should be avoided. Glycerol, gelatin, and other aqueous based media are recommended.

71885 Permanox™ Microscope Slide 20/pk 60.00 Add to Cart
71886 Permanox™ Microscope Slide 100/pk 223.00 Add to Cart
71888 Polystyrene Microscope Slide 20/pk 60.00 Add to Cart
71889 Polystyrene Microscope Slide 100/pk 228.00 Add to Cart

Poly-L-Lysine Coated and Silane Treated Microscope Slide arrow12Poly-L-Lysine

Our premium glass slides with a frosted end are now available pre-coated with either Poly-L-Lysine or Silane (aminoalkysilane). These slides improve the adhesion of tissues to the slide.

 63410-01 Poly-L-Lysine Coated Slides 72/bx 37.50 Add to Cart
 63410-02 Poly-L-Lysine Coated Slides 144/bx 72.00 Add to Cart
 63411-01 Silane Coated Slides 72/bx 37.50 Add to Cart
 63411-02 Silane Coated Slides 144/bx 72.00 Add to Cart

Technical Tip

Poly-L-Lysine Coating of Slides

Polysine™ Microscope Adhesion Slidearrow12Polysine™ Microscope Adhesion Slide

The permanent bio-adhesive material on our Polysine™ slide attracts frozen and paraffin embedded
sections, centrifuge preparation and cytology smears.

63412-01 Polysine™ Adhesion Slide 72/bx 42.00 Add to Cart
63412-02 Polysine™ Adhesion Slide 2x72/bx 79.00 Add to Cart

arrow12Excell™ Adhesion SlidesExcell™ Adhesion Slides

The Excell slide was developed with superior adhesion and wettabiliy properties, and has been shown to perform very well in most applications

  • Excell™ Adhesion Surface for Heat Induced Epitope Retrieval – Excell™ was developed for use in the HIER methods that require high pH antigen retrieval solutions, including EDTA. Formalin fixed paraffin embedded tissue demonstrated more consistent attachment when performing these procedures on the Excell™ surface.
  • Automated Staining – Excell™ has a uniform hydrophilic surface that works extremely well with automated staining equipment.
  • Cell Culture – Excell™ surface technology conditions the surface to enhance the attachment of anchorage dependent cell lines, including many fastidious lines, such as primary neuronal cells. Attachment and proliferation is increased so cells achieve a confluent monolayer sooner
  • Plastic – Excell™ works well for both Epon and Methyl Methacrylate sections. Its uniformly hydrophilic surface allows the sections to spread well during initial attachment. Section adherence is maintained throughout lengthy staining procedure.
  • Laser Capture Microdissection – Excell™ gives you the advantage of excellent tissue adherence of all tissue types, yet it easily releases those area of interest identified for Laser Capture Microdissection.
64370-01 Excell™ Adhesion Slide 144/pk 106.00 Add to Cart

arrow12Text Box:Adhesion Slide – TruBond™

Adhesive slides with proven performance under high temperature and pH conditions

These adhesive slides, including the hydrophilic TruBond™ 360, work well with automatic IHC applications. EMS is confident that the quality of these slides will exceed your expectations. For problems with tissue loss try our TruBond™ 380.

Available colors: White, Blue, Green and Yellow.

 

  TruBond™ 380 TruBond™ 360 TruBond™ 200 TruView™ Fine
Glass Type High Clarity, Low
Fluorescence White
High Clarity, Low Fluorescence White High Clarity, Low Fluorescence White High Clarity, Low Fluorescence White
Surface Wettability Hydrophilic Hydrophilic Hydrophobic Hydrophilic
Adhesion Very Strong Strong Strong  Good
Edge Treatment Ground Corner Ground Corner Ground Corner Ground Corner
For Manual IHC Yes Yes Yes No
For Automatic IHC Yes Yes Yes No
For Fluorescence Yes Yes Yes Yes
Recommended Applications - HIER processes when tissue type require extra strong adhesion - Epitope enhancement & DNA probe procedures - Autopsy & brain sectioning - All IHC applications - HIER pH 6  & pH 9 protocols -Routine histology - Frozen section - All IHC applications - Routine histology - All standard procedures

Ordering:

TruBond™ is packaged 100 slides per box, 10 boxes to a case.

Item# Description Color 100 slides/bx 10 boxes/cs
63700-Wx TruBond™ 380 White 40.00 Add to Cart 323.00 Add to Cart
63700-Bx TruBond™ 380 Blue 40.00 Add to Cart 323.00 Add to Cart
63700-Gx TruBond™ 380 Green 40.00 Add to Cart 323.00 Add to Cart
63700-Yx TruBond™ 380 Yellow 40.00 Add to Cart 323.00 Add to Cart
63701-Wx TruBond™ 360 White 40.00 Add to Cart 323.00 Add to Cart
63701-Bx TruBond™ 360 Blue 40.00 Add to Cart 323.00 Add to Cart
63701-Gx TruBond™ 360 Green 40.00 Add to Cart 323.00 Add to Cart
63701-Yx TruBond™ 360 Yellow 40.00 Add to Cart 323.00 Add to Cart
63702-Wx TruBond™ 200 White 40.00 Add to Cart 323.00 Add to Cart
63702-Bx TruBond™ 200 Blue 40.00 Add to Cart 323.00 Add to Cart
63702-Gx TruBond™ 200 Green 40.00 Add to Cart 323.00 Add to Cart
63702-Yx TruBond™ 200 Yellow 40.00 Add to Cart 323.00 Add to Cart
63703-Wx TruView™ Fine White 20.00 Add to Cart 160.00 Add to Cart
63703-Bx TruView™ Fine Blue 20.00 Add to Cart 160.00 Add to Cart
63703-Gx TruView™ Fine Green 20.00 Add to Cart 160.00 Add to Cart
63703-Yx TruView™ Fine Yellow 20.00 Add to Cart 160.00 Add to Cart

BrightSlide DPDF Downloadarrow12BrightSlide™ D and BrightSlide™ DB

Fluorescence Enhancing Slides and Versatile, (bio)-functionalized coatings for Glass, Metal Oxides and ITO

Fluorescence systems (e.g. microscopes) are today widely used in the life science research. For many applications an increased sensitivity compared to existing glass slides is required. The BrightSlide™ D technology uses a proprietary dielectric coating to significantly enhance the signal intensity of fluorecence molecules from the surface. It can be read out with all systems that illuminate and detect from the top. The technology makes use of purely optical principles and substitutes conventional glass substrates.

arrow12BrightSlide™ D

The BrightSlide™ D (Dielectric) comes standard as a bare SiO2 Surface.

BrightSlide D
Enhancement of the fluorescence on BrightSlide™ image of 150nm thick section of biological materialBrightSlide D
Fluorescence signal on standard, glass slide image of 150nm thick section of biological material

Benefits

  • Better signal to noise ratio
  • Less sample material can be used
  • More genes can be detected
  • Existing instrument platform can be used
  • Purely optical amplification of fluorescence signal
  • Dielectric, high quality SiO2 surface ready to apply AziGrip4™ or customer bio-functional coating (use your own bio-functional coating, no change of chemical protocol required)

Applications

  • Universal detection platform for fluorescence detection on planar surfaces, e.g. fluorescence detection of ultra thin samples
  • Differential gene expression using Cy3 and Cy5 labels simultaneously
  • Gene expression using Cy3 or Cy5 labels independently
arrow12BrightSlide™ DB with AziGrip4™ Bio-Functional Coating
BrightSlide D

The BrightSlide™ DB slide comes with the Bio-functional coating AziGrip4™.

The AziGrip4™ Bio-functional coating platform is a versatile tool for functionalizing surfaces for Life Science, Fluorescence and Medical Applications. Glass, metal oxides, ITO and plastics can be treated in order to bind different amounts of macromolecules, tune specific cell-adhesion, reduce non- specific binding background, provide better signal-to-noise ratios and patterned surfaces with multi-functionalities.

BrightSlide D
Sketch of the AziGrip4™ Bio-functional coating Two different polymers (non-fouling and bio-functionalized) are linked to the substrate via the AziGrip4™ molecules and form a hydrophilic brush with an end bio- functionalization suitable for use as biosensor, or as specific adhesive site for cell surfaces BrightSlide D
Application example Adhesion sites of Fibroblast grown on 5 x 5 µm adhesive pattern separated by 1 µm Atto633-fluorescently labeled grid.
Courtesy of F. Anderegg, ETH Zurich

Benefits

  • Provides amino or carboxy functionalized surfaces for high density binding of biomolecules through standard procedures (EDC/NHS)
  • Enables direct linking of macromolecules without further functionalization
  • Improves adhesion of cells by use of specific binding sequences
  • Simplifies depositions of phospholipid by-layers
  • Reduces unwanted, non-specific binding to improve signal-to-noise
  • Provides patterned surfaces with multi-functionalities

Applications

  • Diagnostics
  • Bio-analytics
  • Lab-on-a-chip
  • Fluorescence microscopy
  • Cell culture
  • Support for lipidic membranes and other emerging application

The following is the standard configurations of the slides:

Specifications

Optical Coating (both slides unless noted)
- Optimization

488nm and 590nm excitation wavelengths (other wavelengths on request)

- Polarization Both s- and p-polarizations can be used for excitation and emission
- Excitation Light 0° - 3° Angle of Incidence
- Background Ultralow Fluorescence
Label Durable, chemically resistant with a unique serial number
Compatibility Suitable for most of commercially available fluorescence microscopes with illumination and detection from the top
Substrate Material D263T eco (other materials on request)
Substrate Format 75 ±0.05mm x 25 ±0.05mm x 1.1 ±0.1mm (other formats on request)
Active Area slide: 63mm x 22mm
optical coating: 5/2x0.4; C2x0.4; E1.0 according to DIN ISO 10110
Chemical Composition (top surface) BrightSlide™ D: Clean, dielectric, SiO2 surface (ITO coating on request)
BrightSlide™ DB: Clean, dielectric, SiO2 surface and AziGrip4™ with Amine functional group (other AziGrip4™ bio-functional coatings, e.g. brush-like hydrophilic, brush-like non-fouling, hydrogel-like, with Carboxy, Epoxy, Biotin, NTA, NHS, Cholesterol, ss-DNA or Peptide bio-functionality on request)
Environmental Stability (optical coating only) Stable against temperatures up to 200°C. All strongly oxidizing cleaning protocols can be used. All organic solvents can be used. Chemical reagents which etch SiO2(HF, NaOH, KOH) can destroy the enhancement effect of the BrightSlide™
Storage Store in clean environment at room temperature
Other -Fluorescence enhancement optimized for one to three customer specific dyes on one slide
-Stringent batch to batch reproducibility
-Packed under clean-room (class 100) conditions
Additional Bio-functional coating-only specifications (BrightSlide™ DB)

-Full surface monolayer with integrated functionality
-Patterned coating available on request
-1-2 nm dry coating thickness
-Robust and chemically stable (increased stability in comparison to aminopropylpolysiloxane)

AziGrip4™ is a Trademark of SuSOS AG

Ordering Information

Catalog# Description Qty. Price  
63426-D BrightSlide™ D each 50.00 Add to Cart
63426-D-10 BrightSlide™ D 10/pk 450.00 Add to Cart
63426-D-100 BrightSlide™ D 100/pk 3900.00 Add to Cart
63426-DB BrightSlide™ DB each 140.00 Add to Cart
63426-DB-10 BrightSlide™ DB 10/pk 1200.00 Add to Cart
63426-DB-100 BrightSlide™ DB 100/pk 5000.00 Add to Cart

Borosilicate Capillary Micro Glass Slidearrow12Borosilicate Capillary Micro Glass Slide

Made from Corning® 7740 borosilicate, low expansion, type I glass.

Micro glass slides are precision rectangular glass capillary tubes for use in microscopic viewing of liquid samples. Capillary action instantly collects the sample and the thin glass walls minimize evaporation. The design of these cells eliminates the needs for cover glass and the hanging drop method. These micro glass slides can withstand heat up to 500°C. Samples can be retained for future reference by sealing or cementing open ends. Micro glass slide are packaged in plastic vials.

I.D. tolerance ± 10%, Wall tolerance ± 20%

Catalog# Dimensions ID mm
Path x width
Wall
Thickness
Length
mm
Qty. Price  
63820-05 0.02 x 0.2 0.020 50 30/vial 34.00 Add to Cart
63820-10 0.02 x 0.2 0.020 100 30/vial 59.00 Add to Cart
63821-05 0.03 x 0.3 0.025 50 33/vial 40.00 Add to Cart
63821-10 0.03 x 0.3 0.025 100 33/vial 70.00 Add to Cart
63822-05 0.04 x 0.4 0.030 50 36/vial 39.00 Add to Cart
63822-10 0.04 x 0.4 0.030 100 36/vial 71.00 Add to Cart
63823-05 0.05 x 0.5 0.040 50 44/vial 53.00 Add to Cart
63823-10 0.05 x 0.5 0.040 100 44/vial 99.00 Add to Cart
63824-05 0.10 x 1.0 0.090 50 40/vial 48.00 Add to Cart
63824-10 0.10 x 1.0 0.090 100 40/vial 88.00 Add to Cart
63825-05 0.20 x 2.0 0.150 50 36/vial 44.00 Add to Cart
63825-10 0.20 x 2.0 0.150 100 36/vial 78.00 Add to Cart
63826-05 0.30 x 3.0 0.300 50 31/vial 40.00 Add to Cart
63826-10 0.30 x 3.0 0.300 100 31/vial 68.00 Add to Cart
63827-05 0.40 x 4.0 0.400 50 28/vial 49.00 Add to Cart
63827-10 0.40 x 4.0 0.400 100 28/vial 90.00 Add to Cart
63828-10 0.50 x 5.0 0.300 100 14/vial 64.00 Add to Cart
63829-10 0.60 x 6.0 0.400 100 12/vial 53.00 Add to Cart
63830-10 0.70 x 7.0 0.460 100 9/vial 40.00 Add to Cart
63831-10 0.80 x 8.0 0.540 100 7/vial 55.00 Add to Cart
63832-10 0.90 x 9.0 0.600 100 6/vial 42.00 Add to Cart
63833-10 1.00 x 10.0 0.670 100 4/vial 45.00 Add to Cart
63840-05 0.05 x 1.0 0.030 50 50/vial 55.00 Add to Cart
63840-10 0.05 x 1.0 0.030 100 50/vial 107.00 Add to Cart
63841-05 0.10 x 2.0 0.100 50 45/vial 53.00 Add to Cart
63841-10 0.10 x 2.0 0.100 100 45/vial 97.00 Add to Cart
63842-05 0.20 x 4.0 0.150 50 36/vial 45.00 Add to Cart
63842-10 0.20 x 4.0 0.150 100 36/vial 81.00 Add to Cart
63843-05 0.30 x 6.0 0.300 50 24/vial 41.00 Add to Cart
63843-10 0.30 x 6.0 0.300 100 24/vial 73.00 Add to Cart
63844-05 0.40 x 8.0 0.400 50 18/vial 41.00 Add to Cart
63844-10 0.40 x 8.0 0.400 100 18/vial 73.00 Add to Cart

twin adhesive well stickers (1cm diameter) firmly adhere to microscope slidesarrow12Microscope Slide-Wells

Disposable Liquid-Holding Wells

Convenient Pre-cut, twin adhesive well stickers (1cm diameter) firmly adhere to microscope slides.  Examine specimens with or without cover slips.  Available in depths of 0.25mm and 0.50mm.

Multiple Applications Examine or count tissue culture cells, yeast, bacteria and other  microorganisms.  Piggy-back with Microscope Slide-Grids to facilitate counting and sizing.

Versatile - Compatible with different styles of microscopes (conventional, inverted or dissecting).

Chemically Inert - Made of polyester with an acrylic adhesive. Microscope Slide-Wells are inert and stable under most laboratory conditions.

70366-12 Slide Wells, 0.25mm thick 25/pk 44.00 Add to Cart
70366-13 Slide Wells, 0.50mm thick 25/pk 53.50 Add to Cart

Disposable Adhesive Gridsarrow12Microscope Slide-Grids

Disposable Adhesive Grids

Convenient - Easy-to adhere stickers having side-by-side twin grids (20mm x 20mm each with 1mm line spacing) for microscope slides.  Count or measure cells and estimate the size or length of microscopic or semi-micro objects

Versatile - Compatible with different styles of microscopes (conventional, inverted or dissecting).  Virtually all cells and specimens placed on top of Slide-Grids can be counted and/or sized.

Dry Or Wet - Use with dry specimens or suspended specimens such as cells held in buffer under cover slips.  Separate cover slips can be placed over each Slide-Grid.

Chemically Inert - Made of polyester with an acrylic adhesive. Slide-Grids are inert and stable under most laboratory conditions.

70366-14 Slide Grids, 20mm x 20mm 40/pk 28.00 Add to Cart

Grided Microscope Slidesarrow12Gridded Microscope Slides

These 3x1” slides have an indexed grid on the reverse side of the frosted end. The ink is resistant to stains and common laboratory solvents. Each square grid fills one low-powered-field (approximately 100X magnification). These slides make it easy to locate an organism or cell every time. There are sixty-four squares in total. The slides are available with coatings of Poly-L-Lysine or Silane, or they can be purchased plain (uncoated).

The grid measures 2 mm square-16mm total. Numbers 1-8 and alpha A-H.

arrow101. Gridded Microscope Slides without hydrophobic coating.
63405-01 Grid Slides, Uncoated 72/pk 58.00 Add to Cart
63405-02 Grid Slides, Uncoated 144/pk 108.00 Add to Cart
63406-01 Grid Slides, Poly-L-Lysine 72/pk 58.00 Add to Cart
63406-02 Grid Slides, Poly-L-Lysine 144/pk 108.00 Add to Cart
63407-01 Grid Slides, Silane 72/pk 59.00 Add to Cart
63407-02 Grid Slides, Silane 144/pk 112.00 Add to Cart
arrow102. Gridded Microscope Slides with hydrophobic coating.
63408-01 Grid Slides, Round well 72/pk 69.00 Add to Cart
63409-02 Grid Slides, Square well 72/pk 69.00 Add to Cart

PTFE Printed Slidesarrow12"PTFE" Printed Slides

"PTFE" Printed Slides, are extremely hydrophobic, acetone proof, chemical resistant and autoclavable. They are perfect for controlling cross-contamination in staining, immunolabeling and immunofluorescence tests. All printed slides are precleaned. The well surface is 100% wettable, which enhances cell attachment and increases its spreading capabilities within the well. These slides minimize the use of expensive purified antigens and antibodies. The slides measure 3x1" (76x26x1mm).

EMS Printed Slides are manufactured to order offering maximum shelf life. The shelf life of "PTFE" Printed Slides range from 18 months to two years. However, it is recommended that all glass slides (Printed or Un-printed) be used in a timely manner to avoid glass "weathering", (slides becoming cloudy or hazy even with proper storage. All of our Printed Slides are autoclavable and have a frosted end. They are intended for one time use only.

Features:

  • Super Hydrophobic Mask
  • Clean, Wettable Wells
  • A Bioadhesive Surface Treatment to Enhance Cell and Tissue Attachment
Catalog No. Number of Well Well Size Pack Price  
63414-13 1 Ring 13 mm Dia. 72 60.00 Add to Cart
63414-10 Square 10.7x10.7mm 72 60.00 Add to Cart
63415-12 1 Round 12 mm Dia. 72 60.00 Add to Cart
63415-32 1 Oval 24.4x16.7mm 72 60.00 Add to Cart
63415-08 1 Round 8 mm Dia. 72 60.00 Add to Cart
63415-15 1 Round 15 mm Dia. 72 60.00 Add to Cart
63416-08 2 Round 8 mm Dia. 72 60.00 Add to Cart
63416-15 2 Round 15 mm Dia. 72 60.00 Add to Cart
63417-13 2 Ring 13 mm Dia. 72 60.00 Add to Cart
63417-15 2 Square 15x15mm 72 60.00 Add to Cart
63418-11 3 Ring 11.28mm Dia. 72 60.00 Add to Cart
63419-08 3 Round 8 mm Dia. 72 60.00 Add to Cart
63419-10 3 Round 10 mm Dia. 72 60.00 Add to Cart
63419-12 3 Round 12 mm Dia. 72 60.00 Add to Cart
63419-14 3 Round 14 mm Dia. 72 60.00 Add to Cart
63419-VIR 3 Round TGH VIROLOGY 72 60.00 Add to Cart
63420-05 4 Round 5 mm Dia. 72 60.00 Add to Cart
63420-08 4 Round 8 mm Dia. 72 60.00 Add to Cart
63420-11 4 Ring 11.43 mm Dia. 72 60.00 Add to Cart
63421-07 5 Round 7 mm Dia. 72 60.00 Add to Cart
63421-10 5 Round 10 mm Dia. 72 60.00 Add to Cart
63421-11 Etched 5 Round 11.43 mm Dia. 72 60.00 Add to Cart
63423-05 6 Round 5 mm Dia 72 60.00 Add to Cart
63423-08 6 Round 8 mm Dia. 72 60.00 Add to Cart
63422-06 8 Round 6 mm Dia. 72 60.00 Add to Cart
63422-11 8 Round 11 mm Dia. 72 60.00 Add to Cart
63424-06 10 Round 6 mm Dia. 72 60.00 Add to Cart
63425-05 12 Round 5 mm Dia. 72 60.00 Add to Cart
63429-04 21 Round 4 mm Dia. 72 60.00 Add to Cart
63430-04 24 Round 4 mm Dia. 72 60.00 Add to Cart
63434-02 30 Round 2 mm Dia. 72 60.00 Add to Cart

Macro/Latex Agglutinationarrow12Macro/Latex Agglutination

25 to a pack

Catalog Glass Size, Configuration Well/Ring
size mm
Price  
63450-31 4" x 3", 3 ring, yellow 26.4 96.00 Add to Cart
63450-32 4.25" x2", 3 ring, white 26.4 96.00 Add to Cart
63453-61 4.4"x2.1", 6, white oval 17.4 x26 96.00 Add to Cart
63453-63 4.215"x2", 6 white oval 17.4 x26 96.00 Add to Cart
63456-15 4.331"x2.165", 10 ring white 15 96.00 Add to Cart
63459-15 4.75"x3.5", 15 ring white 15 160.00 Add to Cart
63460-15 3"x2"x1.2mm, 15 well 11.28 etched 106.00 Add to Cart

Lovins Micro-Slide Field Finderarrow12Lovins Micro-Slide Field Finder

Marking a microscope slide to identify a particular field of interest for future reference has always been a difficult problem for many microscopists.

The Field Finder has a precision rectangular-coordinate grid pattern, which is accurately positioned on a special microscope slide. The slide has been carefully edge ground to provide straight edges and square corners to ensure repeatable results.

Lovins Micros-Slide Field Finder helps you to index points of interest on a microscope slide the same way you find them on a road map. A standard 75 x 25mm glass slide has a photo etched grid pattern of 1mm squares, subdivided into 0.1 mm intervals. Each square is marked with a letter and a number. You center the detail in the field of the microscope, replace the specimen slide with the field finder, and read the coordinates. Note reading on slide, and you can find that detail immediately by reversing procedure.

The pattern is imaged in vacuum deposited chrome for durability.

72266-01 Micro-Slide Field Finder, 75 x 25 mm each 95.00 Add to Cart

arrow12Retriever Tissue Slides

Retriever Tissue Slides are high adhesive glass microscope slides for paraffin-embedded tissue section, including tissue arrays. The slides were designed and optimized for the EMS Antigen Retriever (62700 series) to preserve the section attachment and tissue morphology during heat-induced epitope recovery in Retriever in pH6 (Low), pH9 (High), or Universal Retriever buffer.

Slides can also be successfully used in any other epitope-recovery technology (autoclave, microwave, proteolitic) and show superior performance over positively charged or polylysine-coated slides, especially in high pH buffers and recovery buffers containing EDTA.

General Properties

  • Retriever Tissue Slides are made from soda lime glass
  • Dimensions approximately 76 x 26mm, Thickness 1.0mm.
  • 90° ground edges
  • Coloured or white marking area 20mm, at one end, on one side
  • Pre-cleaned, ready for use
  • Autoclavable
  • Supplied in plastic boxes of 72 pieces (call for carton price, 20 boxes in a carton)
  • Carry recommended best before date and batch number for comprehensive information and traceability
71880-50 Retriever Tissue Slides 72/pk 85.00 Add to Cart
71880-50-CS Retriever Tissue Slides 20 pk/cs 1,250.00 Add to Cart

Cover Glass and Cover Slips arrow12