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Electron Microscopy Sciences

ImmunoGold Reagents

pdf downloadarrow13Micrographs AURION ImmunoGold Reagents

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Routine paraffin section of Hodgkin lymphoma stained for CD 15. Reed-Sternberg cells show positive staining in the cytoplasm. Products used:

  • Mouse monoclonal CD 15
  • GAM IgG GP-US
  • R-Gent
immuno gold
Combination regular light microscopy and
epi-polarization microscopy
immuno gold
Combination regular light microscopy and
epi-polarization microscopy
immuno gold
Epi-polarization microscopy

Products used: Rabbit polyclonal to alpha-amylase, GAR-GP-Ultrasmall R-Gent

immuno gold
Combination regular light microscopy and
epi-polarization microscopy
immuno gold
Combination regular light microscopy and
epi-polarization microscopy
immuno gold
Epi-polarization microscopy

 

immuno goldImmunogold Silver Staining of E-cadherin on a paraffin section of human skin.

Courtesy of R. Moella, Dept. of Exp. Path., EUR, The Netherlands.

• Mouse monoclonal anti E-cadherin
• GAM lgG UltraSmall
• Aurion R-Gent SE-LM

Immunogold silver staining of alpha-amylase on Lowicryl HM20 section of rat pancreas.
  • Goat-anti-Rabbit, 15nm
immuno gold Immunogold silver staining of alpha-amylase on Lowicryl HM20 section of rat pancreas.
  • Goat-anti-Rabbit
  • Ultra Small R-Gent (Dansher Method)
immuno gold
Pre-embedding Immunogold Labeling of Huntingtin Interacting Protein
3 in Mouse Brain using Aurion GAR Fab-US and Aurion SEEM. Courtesy of
Ms Hong Yi, Emory University, Atlanta GA
Membrane labeling with protein IGSS of tubulin on coverslip culture of PtK2 cells Courtesy of Peter van de Plas, Aurion Costerweg 5, The Netherlands. IGSS of tubulin o

 

AURION R-Gent SE-EM Application Example

aurion

ER exit site in 60 nm-thin cryosection of Hepg2 cells, labeled for COPII (primary antibody against sec23 was obtained by ABR) and detected with Fab-goat-anti-rabbit, conjugated to ultra-small gold, silver enhanced for 30 minutes (from Aurion).

The arrows point to labeled COPII-coats on vesicular and tubular membranes, which are located close to the ER.

The information of a thin section is not sufficient to conclude how the membranes are related to each other- if they are still connected to the ER, or if they are free.

Therefore we performed 3D electron tomography on 400nm thick cryosections, which were labeled similar for COPII (see next picture).

aurionCourtesy of: Dagmar Zeuschner, Judith Klumperman (Department of Cell Biology, UMC Utrecht, The Netherlands) and Willie Geerts, Abraham Koster (Molecular Cell Biology, Utrecht University, The Netherlands)

ER= endoplasmic reticulum
PM= plasma membrane
MVB= multi-vesicular body
Bar = 100 mikrometer

2 views of a model of a COPII-labeled ER-exit site, resolved from 400nm thick cryo-sections of Hepg2 cells, labeled like described for the ultrathin section before.

Note that the labeling for COPII is assessable throughout the section.

ER=light blue

Free membrane carriers of vesicular and tubular shape, partially labeled for COPII=yellow

COPII=silver enhanced-red

 

 

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