Counting Chambers
Counting Chambers from Hausser
These chambers are the finest quality, optically ground, and polished milled glass chambers available. The chamber is diamond etched and has a double improved Neubauer Ruling, which has a worldwide reputation in hospitals and laboratories for unmatched reliability, meeting the most demanding of standards. The standard Hausser blood counting chambers are one piece construction (measuring 75mmx32mmx4.5mm) ensuring long term durability and absolute accuracy in measurement and count.
We offer both the standard (incline)
and the new “V-Load” counting hambers for different charging
methods. The tolerances are ( 2% of the volume). Cell Depth: 0.100
mm ( +/-2%); Volume: 0.1 Microliter Ruling; Pattern: Improved Neubauer,
1/400 Square mm Rulings cover 9 square millimeters. Boundary lines
of the Neubauer rulings are the center lines of the groups of three.
(These are indicated in the illustration ). The central square millimeter
is ruled into 25 groups of 16 small squares, each group separated by
triple lines, the middle one of which is the boundary. The ruled surface
is 0.10 mm below the cover glass, so that the volume over each of 16
small squares is 0.00025 cubic mm.
Made to Last
In addition to the standard ruling patterns which are etched permanently onto the polished surface, we offer the popular “Bright-Line” counting chamber. This bright-line counting chamber allows the ruling to appear brightly illuminated providing sharp contrast to the bright lines and cells, helping the user with accurate counts and less eyestrain. The triple lines show clearly which cells lie within the counting area and eliminate guesswork.We guarantee that all marks are permanent, and can not be removed even after repeated washings.
Other Chambers and Accessories
In addition to the blood counting chambers, we also offer a variety of other counting chambers and accessories which compliment our discipline.
- The Phase Hemacytometer: used for phase microscopy; it is a one piece brightlined hemacytometer with a reduced thickness.
- The Petroff Hausser Counting Chamber: used for bacteria counting.
- The Sperm Counting Chambers: used primarily in fertility testing; it is offered in a series of depths (10, 20, 40 microns).
- The Howard Mold Counter and the Sedgewick Rafter Counting Chamber are also available as well as a series of hemacytometer and counting chamber cover glasses.
Double
Neubauer Ruled Metallized Counting Chamber
An “H” shape moat which forms 2 counting areas are metal
plated and double Neubauer ruled providing sharp bright line contrast
for consistent cell counts. A “V” cut on each area is used
to control the possibility of overflow. Triple dividing lines clearly
show cells within the counting area. The ruled surface is 0.1mm below
the cover glass; liquid capacity is 0.1mm3/mm2 and 0.00025mm3/grid
square.
Ordering: The complete set includes one counting chamber, two 0.5mm cover glasses, one red cell pipette, one white blood cell pipette, tubing, mouthpieces and carrying case
| Catalog # | Description | Hauser No. | Price | |
| 63510-10 | Complete Chamber Set | 3120 | 250.00 |  |
| 63510-11 | Chamber with 2 Cover Glasses | 3100 | 204.00 | |
| 63510-13 | Chamber only | 3110 | 192.00 | |
| 63510-20 | Phase Counting Chamber | 3200 | 219.00 | |
Etched -
Transparent Field with Opaque Pattern Levy Double Neubauer
Ruled Hemacytometer
Double improved Neubauer rulings are diamond etched directly into a polished recess to reduce damage from scratches. Opaque surfaces above and below the counting area offer an easy focal reference. The chamber comes with a quick orientation mark for the microscope. 0.1mm cell depth.
Ordering: The complete set includes one counting chamber, two 0.5mm cover glasses, pipettes, and carrying case.
| Catalog # | Description | Hauser No. | Price | |
| 63511-10 | Complete Set | 3520 | 282.00 | |
| 63511-11 | Chamber with Two Cover Glasses | 3500 | 202.00 | |
| 63511-13 | Chamber only | 3510 | 192.00 | |
Other Counting
Chambers
Fuchs-Rosenthal Counting Chamber:
Designed for counting leukocytes, eosinophils and cells in cerebrospinal fluid. Grid pattern is etched directly onto the glass surface. The chamber is 0.2mm deep
Ordering: Chamber set includes: one chamber and 2 octagonal cover glasses.
| Catalog # | Description | Hauser No. | Price | |
| 63512-10 | Fuchs Resenthal Counting Chamber | 3720 | 256.00 | |
Petroff-Hausser Counting Chamber:
New improved Neubauer ruling pattern
with 1/400 sq. mm of ruling. Designed for the counting of bacteria,
sperm, blood platelets, and the content of vaccines. Cell is 0.2mm
deep with 1.5mm thick glass slide allows the use off dark field microscopy.
| Catalog # | Description | Hauser No. | Price | |
| 63512-20 | Petroff Hausser Chamber | 3900 | 420.00 | |
| 63512-21 | Petroff-Hausser Chamber w/ Case | 3920 | 510.00 | |
| 63512-22 | Petroff-Hausser Chamber no grid, w/ case | 3900SP | 420.00 | |
| 63512-23 | Petroff-Hausser Chamber 10 microns w/ Grid | 3901 | 550.00 | |
| 63512-24 | Petroff-Hausser Chamber 20 microns w/ Grid | 3902 | 550.00 | |
| 63512-25 | Petroff-Hausser Chamber 10 microns w/o Grid | 3951 | 550.00 | |
| 63512-26 | Petroff-Hausser Chamber 20 microns w/o Grid | 3952 | 550.00 | |
Nageotte Counting Chamber:
Bright Line double ruling, 0.5mm cell depth.
| Catalog # | Description | Hauser No. | Price | |
| 63512-30 | Nageotte Chamber with Coverglasses | 4000 | 425.00 | |
Palmer, McMaster, Speirs-Levy and Howard Chambers
| Catalog # | Description | Hauser No. | Price | |
| 63512-50 | Palmer Chamber (Nanoplankton) | 3850 | 185.00 | |
| 63512-75 | McMaster Egg Slide Chamber | 3875 | 282.00 | |
| 63512-56 | Speirs-Levy Eosinophil Counting Chamber | 3556 | 650.00 | |
| 63512-55 | Howard Mold Counter with Two Cover Slips | 3820 | 285.00 | |
Leica Bright-Line
Counting Chamber
Designed by Leica for accurate counting of blood cells platelets, dust/yeast cluster and specimen samples. Sharp contrast from rhodium plated on “V-Load”. Triple dividing lines clearly delineate counting area for accurate and precise results. Molded from a single piece of thermal and shock resistant glass with a polished chamber and cover glass assuring precise capillary loading and even cell distribution. Fluid volume is 0.1 mm3/mm2 and 0.00025mm2 per each square.
Ordering: The complete set includes: one counting chamber, two 0.4mm cover glass, one red cell pipette, one white cell pipette, tubing, mouthpieces, and storage case
| Catalog # | Description | Hauser | Price | |
| 63514-10 | Bright-Line Chamber Set | 1483 | 243.00 |
|
| 63514-11 | Bright-Line Chamber w two 0.4mm Cover Glass | 1492 | 184.00 | |
| 63514-12 | Bright-Line Chamber Only | 1490 | 169.00 | |
| 63514-20 | Bright-Line Phase Chamber Set w/o Cover Glass | 1475 | 197.00 | |
| 63514-30 | Hemacytometer Cover Glass, 0.4mm | 1461 | 22.00 | |
| 63514-31 | Red Cell Pipette (Thoma) | 1456 | 37.00 | |
| 63514-32 | White Cell Pipette (Thoma) | 1458 | 37.00 | |
| 63514-34 | Plastic Case for Hemacytometer | 1505 | 34.00 | |
Accessories
for Counting Chambers
| 63516-10 | Cover Glass 20x26x0.5mm | 5000 | 10.00 | |
| 63516-11 | Cover Glass 25x28x0.5mm | 5010 | 29.00 | |
| 63516-12 | Cover Glass 25x33x0.5mm | 5020 | 29.00 | |
| 63516-13 | Cover Glass 27x37x0.5mm | 5030 | 33.00 | |
| 63516-20 | Sedgewick Rafter Cover Glass | 5040 | 29.00 | |
| 63516-30 | Petroff-Hausser Reinforced Cover Glass | 5050 | 87.00 | |
| 63516-31 | Petroff-Hausser Cover Glass 20x26x0.2mm | 5051 | 32.00 | |
| 63516-40 | Whipple Eyepiece Micrometer | 5060 | 194.00 | |
| 63516-42 | Howard Eyepiece Micrometer | 5070 | 158.00 | |
| 63516-50 | Nageotte Cover Glass, 30x33x0.5mm | 5400 | 34.00 | |
| 63516-61 | Nageotte Cover Glass 30x33x1.5mm | 5411 | 24.00 | |
| 63516-45 | Howard Thin Cover Glass 28 x 33 x 0.5mm Thick | 5080 | 34.00 | |
| 63516-46 | Howard Thick Cover Glass 28 x 33 x 1mm Thick | 5090 | 34.00 | |
Cellometer™- The Disposable Cell Counting
Chamber!!
Cell Counting Made Easy
These Disposable Cell Counters are an enclosed chamber with two ports for sample introduction. The chamber contains precisely spaced lines in a grid pattern. There are two counting chambers per slide for replicates The counting grid pattern is the Improved Neubauer, as in a common Hemacytometer. The consistent pattern design allows the standard cell counting procedure to be followed.
With a pipetor, 15 to 20 microlilter cell-containing sample is introduced into the counting chamber through one of the ports. Liquid spreads inside the chamber, replaces air, which escapes through the opposing port.
The cell counting chamber is placed under a phase contrast optical microscope with a typical magnification of 100. The microscope is focused both onto the grid pattern and the cell particles. Standard protocols are followed for cell counting.
- Count the total number of cells found in 4 large corner squares (C_total)
- Cell concentration = Dilution x C_total / Volume
Let the Cellometer™ replace your Hemacytometers, which are traditionally
used for cell. Counting!!
Benefits of the Cellometer™:
- No washing after counting. Extremely important for people whom work with infectious samples.
- No need to place the cover slide
- Easy sample introduction. By integrating the cover slide into the counting chamber, the Cellometer™ eliminates the mistakes that may be caused by misplacement of the cover slide or misleading of the sample- two major sources for counting errors when using the Hemacytometer.
- Made from plastic. Ideal for BioSafety level 3.
Technical Data
Comparisons of Counting Protocols
| Step | Cellometer™ | Hemacytometer |
| 1 | Mix cell suspension | Mix cell suspension |
| 2 | Pipette 15 to 20 microliter into sample port | Carefully load with 10 microliter solution |
| 3 | Count cells in 4 corner squares (C_total) | Count cells in 4 corner squares (C_total) |
| 4 | Cell Concentration = Dilution x (C_total / 4.0 ) x10 4 | Cell Concentration = Dilution x (C_total / 4.0 ) x10 4 |
| 5 | Dispose used Cellometer | Wash and dry cover slide and base |
Description of the experiment:
- Cell Type: Human T lymphocyte
- Counting Devices: Cellometer™ with glass base (CG2), all plastic Cellometer™ (CP2) and a standard bright line Hemacytometer.
- Cell counting performed alternating between Cellometer™ and Hemacytometer.
- Statistical data analysis using Two-Sample T test.
Results:
| CP2-test 1 | CP2-test 2 | CG2 | |
| # of test (Cellometer™) | 15 | 19 | 17 |
| # of test (Hemacytometer) | 8 | 17 | 17 |
| Mean (Cellometer™) | 1.78 x 10 5 | 1.83 x 10 5 | 1.87 x 10 5 |
| Mean (Hemacytometer) | 1.85 x 10 5 | 1.82 x 10 5 | 1.82 x 10 5 |
| p-value | 0.325 | 0.908 | 0.505 |
Conclusions:
- Cell counts obtained using Cellometer™ and Hemacytometer are consistent.
- With Cellometer™, sample loading is simple and consistent.
- There is no washing and drying after using Cellometer™, which Eliminates liquid waste and Saves time and effort
Ordering Information:
| 63561-25 | Cellometer™ Disposable Cell Counting Chamber Plastic Base | 25/bx | 80.00 | |
| 63561-100 | Cellometer™ Disposable Cell Counting Chamber Plastic Base | 100/bx | 300.00 | |
