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Illuminators, Magnifiers, Microscopes & Graticules

Electron Microscopy Sciences

arrow14Eyepiece Graticules

The standard size of the eyepiece Graticle is 16,19, and 21 mm. All other sizes are available on request.

arrow12 A. SIZE AND POSITION SCALES

arrow12 Lines and Crosses

single lines Graticulearrow10 Single Lines - NE50

For measurement of large objects in conjunction with a graduated mechanical stage, and for alignment as well.

Pattern Description Dia. Catalog Price  
NE50 Single Line, nominal width 0.02mm. Image covers entire field of view. Surface chrome image
16mm 67982-16 90.00
  19mm 67982-19 90.00
  21mm 67982-21 90.00

cross lines graticulearrow10 Cross Lines - NE8, NE81, NE82

Used as NE50 but for measurements in two directions and for sighting and alignment. Image covers the entire field of view.

Pattern Description DIa. Catalog Price  
NE8 Cross lines with surface chrome image. Nominal line width 0.020mm. 16mm 67983-01 90.00
  19mm 67983-05 90.00
  21mm 67983-10 90.00
NE81 Cross lines with surface chrome image. Nominal line width 0.040mm. 16mm 67983-20 90.00
  19mm 67983-25 90.00
  21mm 67983-30 90.00
NE82 Cross lines with surface chrome image. Nominal line width 0.005mm. 16mm 67984-16 90.00
  19mm 67984-19 90.00
  21mm 67984-21 90.00

broken crosslines graticulearrow10 Broken Crosslines - NE56

Used as above. Broken lines enable fine detail to beseen at the breaks. A thin boundary would be lost behind a a continuous line.

Pattern Description DIa. Catalog Price  
NE56 Broken cross lines. Image covers the entire field of view. Surface chrome image.
16mm 67985-16 90.00
  19mm 67985-19 90.00
  21mm 67985-21 90.00

crossed gauge linesarrow10 Crossed Gauge Lines - NE53

Use as crossed lines, but for the measuring of distances between thelines. Greater accuracy can be obtained by locatng the specimen detail between the graticules gauge pair.

Pattern Description DIa. Catalog Price  
NE53 Two vertical lines 0.1mm apart with horizontal line. Image covers the entire field of view. Surface chrome image.
16mm 67986-16 112.00
  19mm 67986-19 112.00
  21mm 67986-21 112.00

horizontal & vertical scalesarrow10 Horizontal & Vertical Scales - NE1, NE2, NE5, NE20, NE28, NE31, NE41

These scales are used for the measuring of lengths of specimen or distances between points on a variety of different shaped objects. NE1scale showed: This eyepiece graticule has an overall length of 10.00mm with 100 subdivisions of 0.1mm. When used with a x10 objective each division will represent 10 microns on the specimen. By dividing the division of the chosen graticule by the magnification of the objective one obtains an approximate value that each division will represent on the stage.

Pattern Description DIa. Catalog Price  
NE1 Horizontal micrometer scale 10mm long with 0.1mm subdivions. Surface chrome image. 16mm 68010-16 91.00
  19mm 68010-19 91.00
  21mm 68010-21 91.00
NE2 Vertical micrometer scale 10mm long with 0.1mm subdivions. Surface chrome image. 16mm 68006-16 106.00
  19mm 68006-19 106.00
  21mm 68006-21 106.00
NE5 Horizontal micrometer scale 5mm long with 0.05mm subdivisions. Surface chrome image. 16mm 67988-16 106.00
  19mm 67988-19 106.00
  21mm 67988-21 106.00
NE20 Horizontal scale 0.1 inch long with 100 subdivions. Surface scale image. 16mm 67987-16 106.00
  19mm 67987-19 106.00
  21mm 67987-21 106.00
NE28 Horizontal micrometer scale. 1mm long with 100 subdivisions. Surface chrome image. 16mm 67992-16 112.00
  19mm 67992-19 112.00
  21mm 67992-21 112.00
NE41 Horizontal micrometer scale 10mm long with 200 subdivisions. Surface chrome image. 16mm 67993-16 106.00
  19mm 67993-19 106.00
  21mm 67993-21 106.00

crossed scales graticulearrow10 Crossed Scales - NE17, NE18

Used as a horizontal and a vertical scale, they are especially useful when interested in measurement in different axis.

Pattern Description DIa. Catalog Price  
NE17 Crossed micrometer scales 10mm long with 0.1mm subdivions. Surface chrome image. 16mm 67989-16 112.00
  19mm 67989-19 112.00
  21mm 67989-21 112.00
NE18 Crossed micrometer scales 5mm long with 0.05mm subdivions. Surface chrome image. 16mm 67994-16 112.00
  19mm 67994-19 112.00
  21mm 67994-21 112.00

scales with crosslinesarrow10 Scales with Crosslines - NE7, NE77

Used as a horizontal and a vertical scale, they are especially useful when interested in measurement in different axis.

Pattern Description DIa. Catalog Price  
NE7 Horizontal micrometer scale 10mm long with crosslines at 0.1mm spacing. Surface chrome image. 16mm 68013-16 106.00
  19mm 68013-19 106.00
  21mm 68013-21 106.00
NE77 Horizontal micrometer scale 5mm long in 100 divisions with crosslines. Surface chrome image. 16mm 68012-16 106.00
  19mm 68012-19 106.00
  21mm 68012-21 106.00

arrow12 Squares and Grids

Note: These scales may need to be calibrated, according to intended use. There are a number of uses for the grids and squares listed and they will largely depend on the individual user's application.

EYEPIECE SCALES:

squared grids graticulearrow10 Squared Grids - NE10, NE11, NE34

Simple grids are convenient for making sketches of the obseved speimen on graph paper. They are also useful for particle counting.

Pattern Description DIa. Catalog Price  
NE10 Grid (net) 0.5mm pitch. Surface chrome image. (Pitch: distance of center bar to center bar) 16mm 68011-16 106.00
  19mm 68011-19 106.00
  21mm 68011-21 106.00
NE11 Grid (net) 1.00mm pitch. Surface chrome image. (Pitch: distance of center bar to center bar) 16mm 68015-16 106.00
  19mm 68015-19 106.00
  21mm 68015-21 106.00
NE34 Grid (net) 0.1mm squares. Surface chrome image. 16mm 68018-16 112.00
  19mm 68018-19 112.00
  21mm 68018-21 112.00

indexed gridsarrow10 Indexed Grids - NE10A, NE11A, NE34A

Useful for particle counting, particularly when reference is needed between workers. Also, they are useful for area of specimen determinations.

Pattern Description DIa. Catalog Price  
NE10A Numbered grid5mm x 5mm. 0.5mm pitch. Marked 1-10 and A-J. Surface chrome image. 16mm 68011-16A 106.00
  19mm 68011-19A 106.00
  21mm 68011-21A 106.00
NE11A Numbered grid 10mm x 10mm. 1.0mm pitch. Marked 1-10 and A-J. Surface chrome image. 16mm 68016-16 90.00
  19mm 68016-19 90.00
  21mm 68016-21 90.00
NE34A Numbered grid 1mm x 1mm. 0.1mm pitch. Marked 1-10 and A-J. Surface chrome image. 16mm 68018-16A 112.00
  19mm 68018-19A 112.00
  21mm 68018-21A 112.00

index gridsarrow10 Indexed Grids - NE35

Useful for area of specimen determinations, especially rectangular shapes, also for particle counting.

Pattern Description DIa. Catalog Price  
NE35 Numbered grid 10mm x 10mm. 1mm indexed squares. Surface chrome image. 16mm 68014-16 112.00
  19mm 68014-19 112.00
  21mm 68014-21 112.00

chessboard squaresarrow10 Chessboard Squares - NE15

The dark squares are translucent. Used as an alternative to simple grids for area of specimen determination and paricle counting. Alternative light and dark squares help to reduce eyestrain.

Pattern Description DIa. Catalog Price  
NE15 Chessboard (net) 2.0mm squares. Surface chrome image. 16mm 68008-16 218.00
  19mm 68008-19 218.00
  21mm 68008-21 218.00

squaresarrow10 Squares - NE38

Combines three areas in one for convenience, giving area ratios A:B of 1:3 and B:C of 1:2.

Pattern Description DIa. Catalog Price  
NE38 Squares 10mm, 7mm & 4mm. Glass sandwich. 16mm 68017-16 112.00
  19mm 68017-19 112.00
  21mm 68017-21 112.00

miller squaresarrow10 Miller Squares - NE57

The ratio of large to small squares is 9:1. Originally designed for hematology, they can be utilized for rapid counting of any evenly spread field of particle.

Pattern Description DIa. Catalog Price  
NE57 Miller 7x7mm grid. Glass sandwich. 16mm 68020-16 112.00
  19mm 68020-19 112.00
  21mm 68020-21 112.00

whipple gridarrow10 Whipple Grid - NE29

Originally designed for water particle analysis, but may be used for other aspects of particle counting. Grid showed: Ratio of full square to smallest is 50:1. Area is 2500:1.

Pattern Description DIa. Catalog Price  
NE29 Whipple grid 100 squares in 7mm area. Surface chrome image. 16mm 68009-16 112.00
  19mm 68009-19 112.00
  21mm 68009-21 112.00

arrow12 Gauges

concentric circlesarrow10 Concentric Circles - NE43, NE43, NE44, NE47

Can be used for two-way measurement when calibrated as a micrometer.

Pattern Description DIa. Catalog Price  
NE42 Concentric circle 0.25mm - 2.5mm diameter. 10 circles. Surface chrome image. 16mm 68019-16 112.00
  19mm 68019-19 112.00
  21mm 68019-21 112.00
NE43 Concentric circle 0.5mm - 5mm diameter. 10 circles. Surface chrome image. 16mm 67997-16 112.00
  19mm 67997-19 112.00
  21mm 67997-21 112.00
NE44 Concentric circle 1mm - 10mm diameter. 10 circles. Surface chrome image. 16mm 67998-16 112.00
  19mm 67998-19 112.00
  21mm 67998-21 112.00
NE47 Concentric circle 2mm - 20mm diameter. 10 circles. Surface chrome image. 16mm 67999-16 112.00
  19mm 67999-19 112.00
  21mm 67999-21 112.00

concentric circlearrow10 Concentric Circle - NE22

This design leaves the circle clear of obstruction. In addition the intermediate lines are broken to improve
ease of reading.

Pattern Description DIa. Catalog Price  
NE22 Concentric circle 0.5mm - 12mm diameter. 24 circles. Surface chrome image. 16mm 68000-16 112.00
  19mm 68000-19 112.00
  21mm 68000-21 112.00

gauge pairsarrow10 Gauge Pairs - NE19

Gauge pairs occupying a field of view of 10mm. Each gauge is proportional to its adjacent number. The
approximately size of the smallest pair = 0.1mm.

Pattern Description DIa. Catalog Price  
NE19 Gauge pairs. Surface chrome image. 16mm 68001-16 106.00
  19mm 68001-19 106.00
  21mm 68001-21 106.00

full protractorarrow10 Full Protractor - NE45

Pattern Description DIa. Catalog Price  
NE45 Full protractor scale 10mm diameter dividedin degrees. Surface chrome image. 16mm 68002-16 112.00
  19mm 68002-19 112.00
  21mm 68002-21 112.00

arrow12 PARTICLE SIZING AND DISTRIBUTING

The use of the eyepiece graticules showed in this section make it possible to analyze specimens containing particles as an alternative, or in addition to, sieving. Graticules for particle size analysis are particularly popular when there are only limited quantities of particles or where particles are smaller than 50 microns in diameter. Typical substances analyzed are sand grains, soil particles, plant seeds, fertilizers, abrasives, liquid droplets, pigments, pulverized coal, silica, fibers and fine dust.
The basic principle employed is to compare particles to the globes and circles of varying sizes that appear on the graticule – dark particles being compared to solids globes, and light or transparent ones to the circles. Naturally the procedure varies with the graticule concerned, more information about which is given below.
Please note that for calibration the circles and globes will represent particles smaller in diameter by the magnification of the objective.

paterson globes and circlesarrow10 Paterson Globes and Circles - NG1

Pattern Description DIa. Catalog Price  
NG1 Paterson globes/circles. Surface chrome image. 16mm 68031-16 216.00
  19mm 68031-19 216.00
  21mm 68031-21 216.00

portonarrow10 Porton - NG2

The circle areas of the Porton greaticules increases with Root 2 progression as do the divisions on the right hand side of the rectangle. These divisions are numbered for convenience. The rectangle size is 4.5mm x .25mm. Circle sizes in microns are 560, 400, 280, 200, 140, 100, 70, 50, and 35. The specimen is racked on the mechanical stage of the microscope and traverses are taken right across thee deposit sizing all the particles encountered.

Pattern Description DIa. Catalog Price  
NG2 Original Porton globes/circles. Surface chrome image. 16mm 68024-16 216.00
  19mm 68024-19 216.00
  21mm 68024-21 216.00

new portonarrow10 New Porton - NG12

The NG12 is particularly useful since the array of globes and circles are conveniently close to where the particles pass. At the end of each band of the sample the mechanical stage is traversed vertically to take in the next band until the whole sample has been covered.

Pattern Description DIa. Catalog Price  
NG12 Modified Porton pattern globes/circles. Surface chrome image. 16mm 68032-16 216.00
  19mm 68032-19 216.00
  21mm 68032-21 216.00

British Standard Graticulearrow10 British Standard Graticule - NG10

In this graticule the circle areas double progressively, hence the diameters alter by Root 2, so that the size classes can form a continuation of the standard series of sieves for particle sizing. Each particle is assigned to a size class defined by two adjacent circles, which represent the size limits of that class. Thus the distribution of size is obtained in terms of the diameter of circles having the same projected area as the particles. This method will cover particles in the range 150 micron to 0.38 micron. The size distributions with respect to their number and weight are determined separately. Final results are calculated as cumulative percents.
Originally designed by the National Coal Board for use in coal mining.

Pattern Description DIa. Catalog Price  
NG10 British Standard (BS3625/BS3260) Globes & Circles. Surface chrome image. 16mm 68026-16 216.00
  19mm 68026-19 216.00
  21mm 68026-21 216.00

fairsarrow10 Fairs - NG5

Designed to extend the sizing range of globe and circle graticules.
Example: Used in conjunction with NG2 the overall size range = 128:1. The circles increase by 2.
Note: Both graticules would have to be used with the same microscope, eyepiece and objective.

Pattern Description DIa. Catalog Price  
NG5 Fairs. Grid length 4mm. Glass Sandwich. 16mm 68033-16 216.00
  19mm 68033-19 216.00
  21mm 68033-21 216.00

walton and beckett graticule for abestosarrow10 Asbestos Fiber Analysis

arrow10 Walton and Beckett Graticule for Asbestos - G22, G24

Calibration factors are required for each of these graticules. See note below.
The Walton and Beckett graticule is used for counting fibrous dust (e.g. asbestos or glass fibers) and is particularly useful where the majority of fibers to be counted are shorter than 5 microns. The circle is divided into four by two diametrical lines scaled in units of 5 and 3 microns respectively. 3 and 5 microns are the critical measurements of fiber lengths and diameters used in fiber counting. Unlike the usual globes of other particle graticules, the Walton and Beckett have a series of shapes to compare objects with. These shapes have been designed for comparison with fibers, especially since they incorporate an aspect ratio of 3:1 or 5:1 essential for such analysis.

IMPORTANT NOTE: The circle on these Walton & Beckett graticules must represent 100 microns at the stage and each one must be manufactured to suit the individual instrument.

Therefore, details should be provided with your order of:

  1. Calibration factor, if known;
  2. Objective magnification;
  3. Eyepiece magnification;
  4. Diameter of graticule disc required;
  5. Microscope make and model.
Pattern Description DIa. Catalog Price  
G22 Walton & Beckett for asbestos. 3:1 ratio. Glass sandwich. 16mm 68028-16 258.00
  19mm 68028-19 258.00
  21mm 68028-21 258.00
G24 Walton & Beckett for asbestos. 5:1 ratio. Glass sandwich. 16mm 68029-16 258.00
  19mm 68029-19 258.00
  21mm 68029-21 258.00
G25 Based on the G22, the G25 is produced by the Institute of Occupational Health. 16mm 68030-16 258.00
  19mm 68030-19 258.00
  21mm 68030-21 258.00

arrow10 Specialist Designs

thompsonarrow10 Thompson - G23

For counting particles in any of three areas of known size. the greaticule is calibrated in the same manner as a normal eyepiece scale. the result is then used to calculate the area of any square.

Pattern Description DIa. Catalog Price  
G23 Thompson for dut analysis 10mm, 7mm and 4mm squares. Glass Sandwich. 16mm 68090-16 260.00
  19mm 68090-19 260.00
  21mm 68090-21 260.00

chalkley point arrayarrow10 Chalkley Point Array - NG52

This is used to quickly determine the relationship of components to each other using random sampling. Curtis gives an example of its application, where a researcher may want to see whether or not a certain drug affects the volume proportion of cell types in a given organ. With this graticule the proportion of points lying over the image of one type of component is statistically proportional to the area occupied by that component. The 25 points of the array are placed over the field of view at random, so that a comparison can be made between the number of points touching the one type of component, with number touching the other type of component in each viewing. A series of observations will yield an increasingly accurate ratio of the comparative incidence of each type of particle.

Pattern Description DIa. Catalog Price  
NG52 Chalkley point array. Surface chrome image. 16mm 68034-16 225.00
  19mm 68034-19 225.00
  21mm 68034-21 225.00

pharmaceutical psa patternarrow10 Pharmaceutical PSA Pattern - G57

This graticule was designed for the pharmaceutical industry. However, it is also useful where particle size considerations are restricted to 10mm and 25mm. Dots and circles give quick references for these two sizes. In addition a scale is incorporated.

The microscope must be calibrated when ordering this graticule, such that the circle must equate to 1mm on the microscope stage.

Pattern Description DIa. Catalog Price  
G57 Pharmaceutical PSA Pattern. Glass Sandwich 19mm 68091-19 260.00
  21mm 68091-21 260.00

counting patternarrow10 Counting Pattern - NG14

Simple counting for geological and soil analysis.

Pattern Description DIa. Catalog Price  
NG14 Counting pattern for soil analysis, 10mm square. Surface chrome image. 16mm 68092-16 228.00
  19mm 68092-19 228.00
  21mm 68092-21 228.00

zeiss integrating disc or henning resseau patternarrow10 Zeiss Integrating Disc 1 or Henning Reseau Pattern 25 Points - G49

Pattern Description DIa. Catalog Price  
G49 Henning Resseau pattern (Zeiss integrating disc 1). Glass Sandwich. 16mm 68093-16 250.00
  19mm 68093-19 250.00
  21mm 68093-21 250.00

Zeiss Integrating Disc Zeiss Integrating Disc 100 Points - G47

Similar ro G49 but extended to 100 points that are indexed.

Pattern Description DIa. Catalog Price  
G47 Zeiss integrating disc 1. Glass Sandwich. 16mm 68094-16 260.00
  19mm 68094-19 260.00
  21mm 68094-21 260.00

arrow12 C. STEREOLOGY

In this simplest form, sterology is the science where information about a three dimensional object is obtained from only a two-dimensional section of that structure.

Measurements are usually made with these graticules in the following manner:

  1. An adequare representation of sections of a specimen is obtained.
  2. The graticule is superimposed upon the specimen (or micrograph/projected image of the section).
  3. Finally, the interaction between the superimposed graticule and teh test sections are recorded
    An overall introduction is given by: L.B. Brianrty. "Stereology: Methods for Quantitative Light and Electron Microscopy."

mertz graticulearrow10 The Mertz Graticule (36 Points) - NGM1

Used to estimate the three-dimensional surface areas or the surface density of a component in a given volume, when the component does not have a random orientation. It comprises a test system with parallel curved lines used for measuring the intersection of points.

Pattern Description DIa. Catalog Price  
NGM1 Mertz for Stereology. Surface chrome image. 16mm 68035-16 230.00
  19mm 68035-19 230.00
  21mm 68035-21 230.00

weibel 1arrow10 Weibel 1 - NGW1

Consists of 15 lines of equal length connecting the verticals of a regular hexagonal point network.

Pattern Description DIa. Catalog Price  
NGW1 Weibel Type 1 for Stereology 16mm 68022-16 225.00
  19mm 68022-19 225.00
  21mm 68022-21 225.00

weibel 2arrow10 Weibel 2 - NGW2

Used when making a surface to volume ratio of a structure per mass unit. This graticule consists of a number of short lines with interruptions as long as the lines. Basically, the number of intersections falling over the short lines is counted and the number of endpoints falling on the end of the structure is determined.

Pattern Description DIa. Catalog Price  
NGW2 Weibel Type 2 for Stereology. Surface chrome image. 16mm 68025-16 225.00
  19mm 68025-19 225.00
  21mm 68025-21 225.00

weibel 3arrow10 Weibel 3 - GW3

Pattern Description DIa. Catalog Price  
NGW3 Mertz for Stereology. Surface chrome image. 16mm 68023-16 225.00
  19mm 68023-19 225.00
  21mm 68023-21 225.00

arrow12 D. METALLURGY

Standard pattern discs for metallurgical stereometric analysis of grain size in polished metal sections.

astm austenite grain sizing discarrow10 ASTM Austenite 1:1 Grain Sizing Disc - G41

Pattern Description DIa. Catalog Price  
G41 ASTM Grain Size austenite. Glass sandwich. 19mm 68065-19 250.00
  21mm 68065-21 250.00

astm e112 plate 1 grain sizing discarrow10 ASTM E112 Plate 1 Grain Sizing Disc - G42

Pattern Description DIa. Catalog Price  
G42 Grain sizing (E112). Glass Sandwich. 19mm 68066-19 250.00
  21mm 68066-21 250.00

astm carbide grain sizing chartarrow10 ASTM Carbide Grain Sizing Chart - G43

Pattern Description DIa. Catalog Price  
G43 ASTM Grain Sizing carbide. Glass sandwich. 19mm 68067-19 250.00
  21mm 68067-21 250.00

astm e45arrow10 ASTM E45 - G44

Pattern Description DIa. Catalog Price  
G44 ASTM E45. Root 2 sides. 7.1mm square. 10mm scale. 19mm 68068-19 250.00
  21mm 68068-21 250.00

astm e19-46 grain sizing discarrow10 ASTM E19-46 Grain Sizing Disc - G45

Pattern Description DIa. Catalog Price  
G45 ASTM Grain Sizing E19-46. Glass sandwich. 19mm 68069-19 250.00
  21mm 68069-21 250.00

astm e19-46 grain sizing disc root 2arrow10 ASTM E19-46 Grain Sizing Disc Root 2 - G46

Pattern Description DIa. Catalog Price  
G46 ASTM Grain sizing E19-46 Root 2. Glass sanwich. 19mm 68070-19 250.00
  21mm 68070-21 250.00

circular grid astm 24 pointsarrow10 Circular Grid ASTM 24 Points - G54

Pattern Description DIa. Catalog Price  
G54 ASTM 24 Point Circular Grid. Glass sandwich. 16mm 68084-16 250.00
  19mm 68084-19 250.00
  21mm 68084-21 250.00

square grid astm 25 pointsarrow10 Square Grid ASTM 25 Points - G55

Pattern Description DIa. Catalog Price  
G55 ASTM 25 Point Square Grid. Glass sandwich. 16mm 68085-16 250.00
  19mm 68085-19 250.00
  21mm 68085-21 250.00

Stage Graticules arrow12arrow12

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