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Electron Microscopy Sciences

Illuminators, Magnifiers, Microscopes, & Graticles

arrow13NIGHTSEA™ Fluorescence Viewing Systems

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PDF DownloadTraining ManualNIGHTSEA Stereo Microscope Fluorescence Adapter arrow12NIGHTSEA™ Stereo Microscope Fluorescence Adapter

  • SFA on Microscope, Angle
  • SFA on Microscope, Close-up
  • SFA on Microscope, Close-up
  • Microscope, no SFA
  • Microscope, no SFA, angle
  • Microscope, no SFA, Close-up
  • Leica EZ4 Adapter Version

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Adapt your existing lab stereo microscopes for fluorescence

The NIGHTSEA™ Stereo Microscope Fluorescence Adapter adapts just about any stereo microscope (dissecting microscope) for fluorescence with no modification to the microscope itself. The modular design lets you easily switch between several different excitation/emission combinations to work with a variety of fluorescent proteins and other fluorophores. There are now six different excitation/emission combinations available, plus white light.

Applications

This simple system is excellent for

  • Quick screening of your fluorescent genotypes -Drosophila, zebrafish, C. elegans,...
  • Genotype sorting
  • Fluorescence-aided dissection, injection, or micromanipulation
  • Freeing up your research-grade fluorescence microscopes for more demanding work
  • New faculty start-up budgets
  • Bringing fluorescence into the teaching laboratory

NIGHTSEA Lighthead, Barrier Filter, Filter Shield
Mouse with actin labeled with GFP. Photo © NIGHTSEA™/Charles Mazel NIGHTSEA Lighthead, Barrier Filter, Filter Shield
Mouse with actin labeled with DsRed. Photo © NIGHTSEA™/Charles Mazel NIGHTSEA Lighthead, Barrier Filter, Filter Shield
GFP-tagged Drosophila larva. Photo © NIGHTSEA™/Charles Mazel adapter

microscope adapter

Original Mount Adapter

Leica EZ4 adapter

Leica EZ4 Mount Adapter

Fluorescence isn't just for research microscopes anymore...

  • Now sort on your laboratory-level stereos
  • Use to facilitate micromanipulation and dissection
  • Expand from your research lab to your classroom

Modular...

  • Installs in seconds - just clicks into place
  • Interchangeable excitation/emission combinations
  • Move from microscope to microscope
  • No modification to your micoscope needed

Economical - More Glow for the Dough...

  • Stretch your lab budget
  • Inexpensive enough for classroom use

Grows as your lab grows...

  • Buy just what you need now (up to 5 different wavelength sets)
  • Add more as your needs expand

The Stereo Microscope Fluorescence Adapter system consists of

  • Flexible gooseneck lamp base with power supply
  • Light head
  • Ring adapter for microscope (also available for Leica EZ4 series)
  • Barrier filter
  • Filter shield

The light head, barrier filter, and filter shield are interchangeable so that you can easily switch between excitation/emission light+filter combinations.

The microscope mounting adapter fits up to 67mm to work with the majority of stereo microscopes. An oversize adapter and an adapter for the Leica EZ4 series are also available.

Once you are set up for one excitation/ emission wavelength combination, additional combinations can be added by purchasing a kit that consists of a light head, barrier filter, and viewing shield. These three elements can be removed and replaced in seconds, and color coding ensures that you are using the right combination. The barrier filter clicks on to the ring adapter magnetically, so it is easy to remove it to switch back to white light viewing.

Specifications

Filter Set Abbr Excitation Emission Fluorophores
Royal Blue RB 440-460nm 500nm LP GFP, eGFP, fluorescein...
Green Only RB-GO 440-460nm 500-560nm BP GFP, eGFP, fluorescein...
Cyan CY 490-515nm 550nm LP YFP, Venus, Lucifer Yellow
Green GR 510-540nm 600nm LP DsRed, dTomato...
Violet VI 400-415nm 460nm LP CFP, ...
Ultraviolet UV 360-380nm 415nm LP DAPI, ...

Microscope Mounting Adapter - standard size fits up to 67mm. Also available for oversize (up to 84mm) and Leica EZ4 Series.

Technical Data SheetGreen-Only Barrier Filter

The Green-Only Barrier Filter isolates the green part of the spectrum and is for use with the Royal Blue excitation source. While our other barrier filters are long-pass filters this filter is a bandpass, transmitting from approximately 500 to 560nm. The long-pass filter has served well for most users who need to visualize green-fluorescent protein (GFP), and if you are exploring fluorescence in nature it is preferable. The primary motivation for adding the green-only filter to the line-up was for the benefit of researchers using GFP in plants such as Arabidopsis thaliana, a common research model. Plants contain chlorophyll, which has a distinctive red fluorescence that can sometimes mask the GFP emission, making it harder to see and photograph.

Lamp Base Light Control Options

The NIGHTSEA lamp base is available in three versions: Standard, DIM, and PULSE. Only one control option is available per base. The DIM and PULSE options can be purchased as part of a new system, as a retrofit to an existing base, or in a stand-alone lamp base purchased a la carte.

Standard lamp bases provide a steady light when turned on. DIM lamp bases feature an OFF/ON/DIM switch to change the intensity of the light. PULSE lamp bases incorporate a BNC connector that accepts a voltage signal to control the excitation source ON/OFF.

Dimmer switch option on SFA

Target at max and min excitation

arrow12NIGHTSEA DIM Option

The DIM option adds a combined switch and dimmer control. When you first turn the unit on it is at full power. As you rotate the switch the intensity decreases, reaching about 30% power at the minimum setting. You have finer control in the brighter portion of the adjustment range. An intensity indicator (1-10) makes it easy to record and repeat preferred settings.

BNC input connector for external controlBNC input connector for external control

arrow12 NIGHTSEA PULSE Option

The PULSE option adds a BNC connector on the rear of the base that accepts a user-supplied voltage input to turn the light on and off. Some possible applications are

  • External control (computer, function generator, etc.) for precise illumination timing for photoactivation, behavioral experiments
  • PWM input for light dimming
  • Footswitch for hands-free operation - contact EMS for details

Specifications

  • Voltage input - 2.8 - 6.0 VDC for ON, <0.6VDC OFF
  • Maximum operation frequency - 10kHz
  • BNC cable not included

NIGHTSEA Product Line - Additional Information

Frequently Asked Questions
Instruction Manual (PDF)
Mazel, C., 2013. Adding Fluorescence to Stereo Microscopes. Microscopy Today, 21(5): 12-17. (PDF)

tds

Citations

Aitken, J., V. Ramnath, V. Feygels, A. Mathur, M. Kim, J. Y. Park, and G. Tuell, 2010. Prelude to CZMIL: seafloor imaging and classification results achieved with CHARTS and the rapid environmental assessment (REA) processor. Proc. SPIE 7695, Algorithms and Technologies for Multispectral, Hyperspectral, and Ultraspectral Imagery XVI.

Anastasio, N. C., et al., 2013. Functional status of the serotonin 5-HT2C receptor (5-HT2CR) drives interlocked phenotypes that precipitate relapse-like behaviors in cocaine dependence. Neuropsychopharmacology (13 August 2013). doi:10.1038/npp.2013.199.

Baillie, L. D., V. Hagen, K,. M. Gardner, and S. J. Mulligan, 2011. Functional imaging within individual pain fibres ex vivo with optical microscopy. Journal of Neuroscience Methods, 198(2): 274-279....more citations

Ordering Information

NIGHTSEA Stereo Microscope Fluorescence Adapterarrow12Full System with Original Stereo Microscope Adapter system

Full system with one illumination color consisting of

  • Lamp Base with Power Supply (select lamp base type first)
  • Light Head-Royal Blue, Cyan, Green, Violet or Ultraviolet
  • Microscope Mounting adapter
  • Barrier Filter
  • Viewing Shield
Standard Lamp Base
SFA-RB Full System with Royal Blue, Standard lamp base each 1,095.00 Add to Cart
SFA-RB-GO Full System with Royal Blue, Green-Only Barrier Filter, Standard lamp base each 1,095.00 Add to Cart
SFA-CY Full System with Cyan, Standard lamp base each 1,095.00 Add to Cart
SFA-GR Full System with Green, Standard lamp base each 1,095.00 Add to Cart
SFA-VI Full System with Violet, Standard lamp base each 1,095.00 Add to Cart
SFA-UV Full System with Ultraviolet, Standard lamp base each 1,245.00 Add to Cart
DIM Lamp Base
SFA-RB-DIM Full System with Royal Blue, DIM lamp base each 1,345.00 Add to Cart
SFA-RB-GO-DIM Full System with Royal Blue, Green-Only Barrier Filter, DIM lamp base each 1,345.00 Add to Cart
SFA-CY-DIM Full System with Cyan, DIM lamp base each 1,345.00 Add to Cart
SFA-GR-DIM Full System with Green, DIM lamp base each 1,345.00 Add to Cart
SFA-VI-DIM Full System with Violet, DIM lamp base each 1,345.00 Add to Cart
SFA-UV-DIM Full System with Ultraviolet, DIM lamp base each 1,495.00 Add to Cart
PULSE Lamp Base
SFA-RB-PULSE Full System with Royal Blue, PULSE lamp base each 1,345.00 Add to Cart
SFA-RB-GO-PULSE Full System with Royal Blue, Green-Only Barrier Filter, PULSE lamp base each 1,345.00 Add to Cart
SFA-CY-PULSE Full System with Cyan, PULSE lamp base each 1,345.00 Add to Cart
SFA-GR-PULSE Full System with Green, PULSE lamp base each 1,345.00 Add to Cart
SFA-VI-PULSE Full System with Violet, PULSE lamp base each 1,345.00 Add to Cart
SFA-UV-PULSE Full System with Ultraviolet, PULSE lamp base each 1,495.00 Add to Cart
Leica EZ4 versionadapterfilter on adapter

arrow12Full system with Leica EZ4 Adapter

Full system with one illumination color consisting of:
  • Lamp Base with Power Supply (select lamp base type first)
  • Light Head-Royal Blue, Cyan, Green, Violet or Ultraviolet
  • Leica EZ4 Mounting adapter
  • Barrier Filter
  • Viewing Shield

The Leica EZ4 Mounting Adapter enables you to use the NIGHTSEA Stereo Microscope Fluorescence System with the Leica EZ4 series of stereo microscopes, with or without integrated camera. Easily attach the barrier filter magnetically and hold the filter shield in place with a thumbscrew. The adapter is available for purchase separately to quickly move between Leica and other stereo microscopes (see below).

Standard Lamp Base
SFAZ-RB Full System with Royal Blue, Standard Lamp Base each 1,095.00 Add to Cart
SFAZ-RB-GO Full System with Royal Blue, Green-Only Barrier Filter, Standard Lamp Base each 1,095.00 Add to Cart
SFAZ-CY Full System with Cyan, Standard Lamp Base each 1,095.00 Add to Cart
SFAZ-GR Full System with Green, Standard Lamp Base each 1,095.00 Add to Cart
SFAZ-VI Full System with Violet, Standard Lamp Base each 1,095.00 Add to Cart
SFAZ-UV Full System with Ultraviolet, Standard Lamp Base each 1,245.00 Add to Cart
DIM Lamp Base
SFAZ-RB-DIM Full System with Royal Blue, DIM Lamp Base each 1,345.00 Add to Cart
SFAZ-RB-GO-DIM Full System with Royal Blue, Green-Only Barrier Filter, DIM Lamp Base each 1,345.00 Add to Cart
SFAZ-CY-DIM Full System with Cyan, DIM Lamp Base each 1,345.00 Add to Cart
SFAZ-GR-DIM Full System with Green, DIM Lamp Base each 1,345.00 Add to Cart
SFAZ-VI-DIM Full System with Violet, DIM Lamp Base each 1,345.00 Add to Cart
SFAZ-UV-DIM Full System with Ultraviolet, DIM Lamp Base each 1,495.00 Add to Cart
PULSE Lamp Base
SFAZ-RB-PULSE Full System with Royal Blue, PULSE Lamp Base each 1,345.00 Add to Cart
SFAZ-RB-GO-PULSE Full System with Royal Blue, Green-Only Barrier Filter, PULSE Lamp Base each 1,345.00 Add to Cart
SFAZ-CY-PULSE Full System with Cyan, PULSE Lamp Base each 1,345.00 Add to Cart
SFAZ-GR-PULSE Full System with Green, PULSE Lamp Base each 1,345.00 Add to Cart
SFAZ-VI-PULSE Full System with Violet, PULSE Lamp Base each 1,345.00 Add to Cart
SFAZ-UV-PULSE Full System with Ultraviolet, PULSE Lamp Base each 1,495.00 Add to Cart
Leica Adapter Only
SFAZ-AD Leica EZ4 Adapter only each 168.00 Add to Cart

Accessories

Add-On Light and Filter Setarrow12Add-On Light and Filter Sets
  • Light Head, Royal Blue
  • Light Head, Royal Blue, Angle
  • Light Head, Cyan
  • Light Head, Green
  • Light Head, Violet
  • Light Head, Ultraviolet
  • Barrier Filter, Royal Blue
  • Barrier Filter, Cyan
  • Barrier Filter, Green
  • Barrier Filter, Green Only
  • Barrier Filter, Violet
  • Barrier Filter, Ultraviolet
  • Shield, Royal Blue
  • Shield, Cyan
  • Shield, Green
  • Shield, Green Only
  • Shield, Violet
  • Shield, Ultraviolet

Each add-on excitation/emission set consists of

  • Light Head*
  • Barrier Filter
  • Viewing Shield
  • Padded Storage Box
SFA-BLFS-RB Add-On Light and Filter Set, Royal Blue each 555.00 Add to Cart
SFA-BLFS-CY Add-On Light and Filter Set, Cyan each 555.00 Add to Cart
SFA-BLFS-GR Add-On Light and Filter Set, Green each 555.00 Add to Cart
SFA-BLFS-GO Add-On Light and Filter Set, Green-Only each 555.00 Add to Cart
SFA-BLFS-VI Add-On Light and Filter Set, Violet each 555.00 Add to Cart
SFA-BLFS-UV Add-On Light and Filter Set, Ultraviolet each 705.00 Add to Cart
BNC and V1 Connectors

*Please note: New Light Heads use BNC connectors. The original NIGHTSEA Light Heads used a different connector and are referred to as "V1" . If you wish to purchase a "V1" style light head for an older system, please contact EMS. The image to the right shows the difference between a BNC and "V1" style connector.

More NIGHTSEA™ Accessories arrow13arrow13