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Electron Microscopy Sciences

Illuminators, Magnifiers, Microscopes, & Graticles

arrow13NIGHTSEA™ Fluorescence Viewing Systems

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NIGHTSEA™ / EMS 2017 KEY Award

PDF DownloadNIGHTSEA Stereo Microscope Fluorescence Adapter arrow12NIGHTSEA™ Stereo Microscope Fluorescence Adapter

  • SFA on Microscope, Angle
  • SFA on Microscope, Close-up
  • SFA on Microscope, Close-up
  • Microscope, no SFA
  • Microscope, no SFA, angle
  • Microscope, no SFA, Close-up
  • Leica EZ4 Adapter Version

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Adapt your existing lab stereo microscopes for fluorescence

The NIGHTSEA™ Stereo Microscope Fluorescence Adapter adapts just about any stereo microscope (dissecting microscope) for fluorescence with no modification to the microscope itself. The modular design lets you easily switch between several different excitation/emission combinations to work with a variety of fluorescent proteins and other fluorophores. There are now six different excitation/emission combinations available, plus white light.

Applications

This simple system is excellent for:

  • Quick screening of your fluorescent genotypes -Drosophila, zebrafish, C. elegans,...
  • Genotype sorting
  • Fluorescence-aided dissection, injection, or micromanipulation
  • Freeing up your research-grade fluorescence microscopes for more demanding work
  • New faculty start-up budgets
  • Bringing fluorescence into the teaching laboratory

NIGHTSEA Lighthead, Barrier Filter, Filter Shield
Mouse with actin labeled with GFP. Photo © NIGHTSEA™/Charles Mazel NIGHTSEA Lighthead, Barrier Filter, Filter Shield
Mouse with actin labeled with DsRed. Photo © NIGHTSEA™/Charles Mazel NIGHTSEA Lighthead, Barrier Filter, Filter Shield
GFP-tagged Drosophila larva. Photo © NIGHTSEA™/Charles Mazel adapter

microscope adapter

Original Mount Adapter

Leica EZ4 adapter

Leica EZ4 Mount Adapter

Fluorescence isn't just for research microscopes anymore...

  • Now sort on your laboratory-level stereos
  • Use to facilitate micromanipulation and dissection
  • Expand from your research lab to your classroom

Modular...

  • Installs in seconds - just clicks into place
  • Interchangeable excitation/emission combinations
  • Move from microscope to microscope
  • No modification to your micoscope needed

Economical - More Glow for the Dough...

  • Stretch your lab budget
  • Inexpensive enough for classroom use

Grows as your lab grows...

  • Buy just what you need now (up to 5 different wavelength sets)
  • Add more as your needs expand

The Stereo Microscope Fluorescence Adapter system consists of

  • Flexible gooseneck lamp base with power supply
  • Light head
  • Ring adapter for microscope (also available for Leica EZ4 series)
  • Barrier filter
  • Filter shield

The light head, barrier filter, and filter shield are interchangeable so that you can easily switch between excitation/emission light+filter combinations.

The microscope mounting adapter fits up to 67mm to work with the majority of stereo microscopes. A Leica EZ4 adapter is also available.

Once you are set up for one excitation/ emission wavelength combination, additional combinations can be added by purchasing a kit that consists of a light head, barrier filter, and viewing shield. These three elements can be removed and replaced in seconds, and color coding ensures that you are using the right combination. The barrier filter clicks on to the ring adapter magnetically, so it is easy to remove it to switch back to white light viewing.

Specifications

Filter Set Abbr Excitation Emission Fluorophores
Royal Blue RB 440-460nm 500nm LP GFP, eGFP, fluorescein...
Green Only RB-GO 440-460nm 500-560nm BP GFP, eGFP, fluorescein...
Cyan CY 490-515nm 550nm LP YFP, Venus, Lucifer Yellow
Green GR 510-540nm 600nm LP DsRed, dTomato...
Violet VI 400-415nm 460nm LP CFP, ...
Ultra Violet UV 360-380nm 415nm LP DAPI, ...

Microscope Mounting Adapter - original fits up to 67mm standard. Also available for Leica EZ4 Series

Technical Data SheetGreen-Only Barrier Filter

The Green-Only Barrier Filter isolates the green part of the spectrum and is for use with the Royal Blue excitation source. While our other barrier filters are long-pass filters this new filter is a bandpass, transmitting from approximately 500 to 560nm. The long-pass filter has served well for most users who need to visualize green-fluorescent protein (GFP), and if you are exploring fluorescence in nature it is preferable. The primary motivation for adding the green-only filter to the line-up was for the benefit of researchers using GFP in plants such as Arabidopsis thaliana, a common research model. Plants contain chlorophyll, which has a distinctive red fluorescence that can sometimes mask the GFP emission, making it harder to see and photograph.

Additional Information

Frequently Asked Questions
Instruction Manual (PDF)
Mazel, C., 2013. Adding Fluorescence to Stereo Microscopes. Microscopy Today, 21(5): 12-17. (PDF)

tds

Citations

Aitken, J., V. Ramnath, V. Feygels, A. Mathur, M. Kim, J. Y. Park, and G. Tuell, 2010. Prelude to CZMIL: seafloor imaging and classification results achieved with CHARTS and the rapid environmental assessment (REA) processor. Proc. SPIE 7695, Algorithms and Technologies for Multispectral, Hyperspectral, and Ultraspectral Imagery XVI.

Anastasio, N. C., et al., 2013. Functional status of the serotonin 5-HT2C receptor (5-HT2CR) drives interlocked phenotypes that precipitate relapse-like behaviors in cocaine dependence. Neuropsychopharmacology (13 August 2013). doi:10.1038/npp.2013.199.

Baillie, L. D., V. Hagen, K,. M. Gardner, and S. J. Mulligan, 2011. Functional imaging within individual pain fibres ex vivo with optical microscopy. Journal of Neuroscience Methods, 198(2): 274-279....more citations

Ordering Information

NIGHTSEA Stereo Microscope Fluorescence Adapterarrow12Full System with Original Stereo Microscope Adapter system

Full system with one illumination color consisting of:
  • Lamp Base with Power Supply
  • Light Head-Royal Blue, Cyan, Green, or Violet
  • Microscope Mounting adapter
  • Barrier Filter
  • Viewing Shield
SFA-RB Full System with Royal Blue each 1,095.00 Add to Cart
SFA-RB-GO Full System with Royal Blue Light Head, Green-Only Barrier Filter each 1,095.00 Add to Cart
SFA-CY Full System with Cyan each 1,095.00 Add to Cart
SFA-GR Full System with Green each 1,095.00 Add to Cart
SFA-VI Full System with Violet each 1,095.00 Add to Cart
SFA-UV Full System with Ultra Violet each 1,245.00 Add to Cart
Leica EZ4 versionadapterfilter on adapter

arrow12Full system with Leica EZ4 Adapter

Full system with one illumination color consisting of:
  • Lamp Base with Power Supply
  • Light Head-Royal Blue, Cyan, Green, or Violet
  • Leica EZ4 Mounting adapter
  • Barrier Filter
  • Viewing Shield

The Leica EZ4 Mounting Adapter enables you to use the NIGHTSEA Stereo Microscope Fluorescence System with the Leica EZ4 series of stereo microscopes, with or without integrated camera. Easily attach the barrier filter magnetically and hold the filter shield in place with a thumbscrew. The adapter is available for purchase separately to quickly move between Leica and other stereo microscopes.

SFAZ-RB Full System with Royal Blue each 1,095.00 Add to Cart
SFAZ-RB-GO Full System with Royal Blue Light Head, Green-Only Barrier Filter each 1,095.00 Add to Cart
SFAZ-CY Full System with Cyan each 1,095.00 Add to Cart
SFAZ-GR Full System with Green each 1,095.00 Add to Cart
SFAZ-VI Full System with Violet each 1,095.00 Add to Cart
SFAZ-UV Full System with Ultra Violet each 1,245.00 Add to Cart
SFAZ-AD Leica EZ4 Adapter only each 168.00 Add to Cart

Accessories

Add-On Light and Filter Setarrow12Add-On Light and Filter Sets
  • Light Head, Royal Blue
  • Light Head, Royal Blue, Angle
  • Light Head, Cyan
  • Light Head, Green
  • Light Head, Violet
  • Light Head, Ultra Violet
  • Barrier Filter, Royal Blue
  • Barrier Filter, Cyan
  • Barrier Filter, Green
  • Barrier Filter, Green Only
  • Barrier Filter, Violet
  • Barrier Filter, Ultra Violet
  • Shield, Royal Blue
  • Shield, Cyan
  • Shield, Green
  • Shield, Green Only
  • Shield, Violet
  • Shield, Ultra Violet

Each add-on excitation/emission set consists of:

  • Light Head*
  • Barrier Filter
  • Viewing Shield
  • Padded Storage Box
BNC and V1 Connectors

*Please note: Light Head connectors come in two types. They are not interchangeable. The new style connector is called a BNC, and the original connector is a V1. There are two differences - the connector on the end of the gooseneck and the mating connector on the back of the light head. It is easy to tell them apart:

  • The BNC connector on the gooseneck is silver, while the V1 connector is black.
  • The BNC light heads have the letters BNC printed on the side.
arrow12BNC Add-On Light and Filter Sets
SFA-BLFS-RB BNC Add-On Light and Filter Set, Royal Blue each 555.00 Add to Cart
SFA-BLFS-CY BNC Add-On Light and Filter Set, Cyan each 555.00 Add to Cart
SFA-BLFS-GR BNC Add-On Light and Filter Set, Green each 555.00 Add to Cart
SFA-BLFS-GO BNC Add-On Light and Filter Set, Green-Only each 555.00 Add to Cart
SFA-BLFS-VI BNC Add-On Light and Filter Set, Violet each 555.00 Add to Cart
SFA-BLFS-UV BNC Add-On Light and Filter Set, Ultra Violet each 705.00 Add to Cart
arrow12V1 Add-On Light and Filter Sets
SFA-LFS-RB V1 Add-On Light and Filter Set, Royal Blue each 555.00 Add to Cart
SFA-LFS-CY V1 Add-On Light and Filter Set, Cyan each 555.00 Add to Cart
SFA-LFS-GR V1 Add-On Light and Filter Set, Green each 555.00 Add to Cart
SFA-LFS-GO V1 Add-On Light and Filter Set, Green-Only each 555.00 Add to Cart
SFA-LFS-VI V1 Add-On Light and Filter Set, Violet each 555.00 Add to Cart
SFA-LFS-UV V1 Add-On Light and Filter Set, Ultra Violet each 705.00 Add to Cart

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