Stage Graticules

These stage graticules are intended for the routine calibration of eyepiece patterns, particularly when alternating between objectives on one microscope or when using the same graticule in different microscopes. Their robust construction makes them ideal for student use and for instructional purposes. The scale or grid is centered on a glass disc mounted in a black anodized aluminum slide 75mm x 24mm x 2mm thick. For applications requiring traceable standards, see page 507.

Two types are available:

• Transmitted light
• Reflected light, as is used with metallurgical microscopes.

A. SCALES AND MICROMETER

For Transmitted Light

Standard and Crossed Patterns - S1, S2, S4, S5, S8, S11, S12, S16, S22, S48

Pattern	Type, Length	Nos. Div.	Div. Size	Catalog	Price
S1	Horizontal, 10mm	100	0.1mm	68040-01	192.00
S2	Horizontal, 5.0mm	100	0.05mm	68039-02	222.00
S4	Horizontal, 0.1"	100	0.001"	68039-04	222.00
S5	Horizontal, 20mm	200	0.01 mm	68039-05	308.00
S8	Horizontal, 1.0mm	100	0.01mm	68042-08	192.00
S11	Horizontal, 0.005"	50	0.0001"	68039-11	310.00
S12	Horizontal, 0.1mm	50	0.002mm	68044-12	310.00
S16	Crossed Scale, 1.0mm	100	0.01mm	68039-16	260.00
S22	Vertical Scale, 2.0mm	200	0.01mm	68039-22	226.00
S48	No Coverglass, 1.0mm	100	0.01mm	68041-48	220.00

Combined Metric/Imperial Pattern

Pattern	Description	Catalog	Price
S20	Double micrometer scale 2mm in 0.01mm divisions and 0.1" in 0.005" divisions.	68043-20	295.00

Grouped Graduation Pattern - S21

For speedy determination of a range of featured sizes within a given specimen.

Pattern	Description	Catalog	Price
S21	Micrometer scale 5mm in 0.5mm divisions, 2mm in 0.1mm divisions, and 0.2mm in 0.01mm divisions.	68043-21	208.00

Grids - S9, S10, S28, S29

Pattern	Description	Catalog	Price
S9	Counting Slide 0.1mm squares.	68045-09	220.00
S10	Counting Slide 0.05mm squares .	68045-10	220.00
S28	0.01mm grid / 0.2 x 0.2mm overall.	68045-28	240.00
S29	0.01mm grid / 1.5 x 1.5mm overall.	68045-29	240.00

Diamond Ruled Stage Micrometer - S91

A stage micrometer with very well defined lines. These reference scales offer substantial improvements in definition over the standard types when used under the highest magnification. This is made possible by the use of diamond cutting tools and a special ruling engine.
The S91 has clear lines ruled through a semi opaque metal film, which with transmitted light, appear as bright lines on a dark background.
The micrometer is made on a glass substrate 76mm x 26mm x 1.2mm thick. The lines are 1 micron wide or less and 3.5mm long. The metric rulings provide a scale of 1mm divided into 0.1mm parts, with one part being sub-divided into 0.001mm parts.

Pattern	Description	Catalog	Price
S91	Diamond ruled stage micrometer	68046-91	300.00

For Reflected Light

This scale is etched through highly reflective vacuum coated metal. When viewed under vertical illumination, as with a metallurgical microscope, the scale appears black against a bright background.

Reflected Light - S78

Pattern	Description	Catalog	Price
S78	Micrometer scale 1mm in 0.01mm divisions.	68043-78	208.00

B. STAGE CALIBRATION STANDARDS

These stage micrometers differ from the standard stage micrometers due to the fact they are being available with a certificate giving precise details about their accuracy. These calbrated standards provide traceability for the precise calibration and confirmation of accuracy of optical measuring instruments, which is necessary under ISO provisions.

Micrometer Standards - PS1, PS4, PS5, PS8, PS12, PS16, PS78

These standards are differ from the stage micrometers listed on page 504. The glass discs are mounted in stainless steel slides with engraved serial numbers. Each slide is supplied in a polished wooden storage case to distinguish it as a traceable standard of high value. We can arrange for the calibration of its scales and grids to be carried out by the most appropriate laboratory to suit the customers requirements – the choice of laboratory is normally dependent on the nature of the calibration and the accuracy required.

1. Calibration by NPL: The National Physical Laboratory carries out measurements at selected points on the scale and grids and issues a certificate of calibration. Order Code NP. The NPL is the English equivalent of NIST.
2. Calibration by UKAS accredited laboratory: A UKAS accredited laboratory carries out measurements at selected points on the scales and grids and issues a calibration certificate. Order Code: NA
3. Measurement by Us: For applications, which do not require the accuracy provided by calibrations carried out by NPL or a UKAS accredited laboratory, We can provide a Certificate of Comparison. The scale or grid is compared with NPL calibrated in-house standards and a statement is provided on the accuracy of the item with respect to these standards.
   Order Code GR

Pattern	Description	Catalog #	Pack	Price
PS1	S1 with Graticules Certificate	68047-1GR		600.00
	S1 with UKAS Certificate	68047-1NA		600.00
	S1 with NPL Certificate	68047-1NP	each	1195.00
PS4	S4 with Graticules Certificate	68047-4GR		595.00
	S4 with UKAS Certificate	68047-4NA		610.00
	S4 with NPL Certificate	68047-4NP	each	1225.00
PS5	S5 with Graticules Certificate	68047-5GR		675.00
	S5 with UKAS Certificate	68047-5NA		750.00
	S5 with NPL Certificate	68047-5NP	each	1300.00
PS8	S8 with Graticules Certificate	68047-8GR		560.00
	S8 with UKAS Certificate	68047-8NA		620.00
	S8 with NPL Certificate	68047-8NP	each	1200.00
PS12	S12 with Graticules Certificate	68047-12GR		680.00
	S12 with UKAS Certificate	68047-12NA		1200.00
	S12 with NPL Certificate	68047-12NP	each	1350.00
PS16	S16 with Graticules Certificate	68047-16GR		675.00
	S16 with UKAS Certificate	68047-16NA		850.00
	S16 with NPL Certificate	68047-16NP	each	1600.00
PS78	S78 with Graticules Certificate	68047-78GR		620.00
	S78 with UKAS Certificate	68047-78NA		625.00
	S78 with NPL Certificate	68047-78NP	each	1250.00

	Certification	Price
68047-C	NAMAS Certification for SI - S70	400.00
68047-Cert	NPL Certification for SI - S70	1,000.00

Image Analysis Standard (Reference Stage Graticule)

This high precision image analysis standard provides four test areas designed for calibrating image analysis systems and identifying deviations and distortions in optical imaging systems.
The standard, which can also be used as a high precision stage micrometer, is supplied with recommendations for its use and an individual certificate of calibration.
It is produced on a 75mm x 25mm slide and has a square grid accuracy of ±0.1µm and a dot accuracy of ±0.3µm (except for the smallest and largest two dots on the root-2 array, where accuracy is ±0.5µm). The four test areas are:

• 400µm x 400µm square grid which is subdivided into 200, 100, 50 and 25 micron squares provides a means of detecting gross image distortions, and can be used as an accurate two dimensional stage micrometer.
• A 20 x 17 array of nominally 15µm diameter dots can be used to identify lens distortions. i.e. to set the field of view to eliminate edge distortion.
• A root-2 array of spots from 3µm to 48µm diameter is used for determining the threshold levels of cameras and microscope systems.
• A log-normal distribution array of 100 spots ranging from 4.5µm to 27µm diameter enables the mean and standard deviation to be determined and compared with the certified values. This is an idealized distribution of maximum dynamic range for a full screen.

Catalog	Description	Price
68049	Reference stage graticule 75mm x 25mm slide.	2700.00

H.S.E./N.P.L. Test Slide for Phase Contrast Microscopy - S84

This test slide is made in the UK and is under license from the National Physical Laboratory. It is an epoxy replica of a master slide produced and certified by that laboratory. The replicas are mounted on microscope slides of 1.2mm thickness with cover glass of 0.17mm thickness. This information is transferred to the accompanying table, which indicates the maximum phase change passing through test objects to test slide. A satisfactory system will detect block 5. Full details are supplied with the slide.

Pattern	Description	Catalog	Price
S84	HSE Test Slide for Calibration in Asbestos Analysis	68038-84H	1,380.00

C. VIBRATION

FOE PPL Dot - S25

The pair of dots, which appear to merge into a single dot, determines the amount of vibration of the slide in the appropriate axis. The pattern on the S25 is an array of 20 pairs of dots converging on a single dot. The distance between each dot pair increases by 0.001 inch to a maximum of 0.02 inch, pairs being squispaced 0.25 inch.

Pattern	Description	Catalog	Price
S25	Foepple Dot Vibration Test	68038-25	280.00

D. VOLUME/COUNTING CELL

These are used to create precise volumes of liquid to enable counting of particles in a specified volume of liquid. A thick slide (glass or plastic) has an area cut into its surface that will hold precise volumes of bathing fluid. The shallow well, facilitating easy counting of contained particles. In use, a single drop of liquid is placed on the slide and a coverglass is placed on the platform providing equal and accurate subdivisions of the fluid each of which can be observed for particle counting.

Sedgewick Rafter and Cover Glass - S50, S51, S52

For use in water analysis, culture inspection, and any fluid where particles per unit volume need to be determined. Available in two versions:
S50 is an economically priced plastic cell.
S52 is made from glass, with a chromium surface image. This is intended for professional use and when using phase contrast. In each type, a central cell is filled with fluid and a coverglass limits the volume to 1 milliliter. The grid subdivides this into micro-liters.
S51 is the spare cover glass for S50 and S52. Each cell is supplied with one cover glass.

Pattern	Description	Catalog #		Price
S50	S50, Sedgewick Rafter, Plastic	68050-50	each	56.00
S52	S52, Sedgewick Rafter, Glass	68050-52	each	250.00
S51	S51, Cover Glass for S50 & S52	68050-51	each	16.00

Howard Cell and Cover Glass - S60, K20

The Howard cell is a glass slide 76mm x 35mm with a central circular island and is used for counting mold fibers and spores in fruit juices, especially from tomatoes. With the K20 coverglass in place a 0.1mm thickness of liquid is contained over the central island. The coverglass has 25 calibrated fields of 1.382mm diameter through which to view the particles. This coverglass removes the necessity of precise adjustment of the microscope and eyepiece graticule in the original Howard method.

Pattern	Description	Catalog #	Price
S60	Howard Cell for Fruit Juices	68051-60	302.00
K20	Sedgewick Rafter Counting Chamber (glass)	68051-20	92.00

E. FINDER GRATICULES

Finder graticules are used to swiftly and accurately give a position of reference to an area of interest on a specimen.

The England Finder – S7

S7 is a glass slide 75mm x 26mm (3x1") marked over the top surface in a way that a referenced position can be directly read relative to the locating edges. That means it is marked with a square grid at 1mm intervals. Each square contains a center ring bearing reference letters and numbers, the remainder of the square being subdivided into four segments numbered 1 to 4.

Reference numbers run horizontally 1 to 75 and letters vertically A to Z (omitting I) The main locating edge is the bottom of the slide, which is used in conjunction with either the left or right vertical edge of the slide.

All England Finders produced for over 40 years are identical. The purpose of the finder is to give the microscopist an easy method of recording the position of a particular field of interest, so that another person in another laboratory can relocate at a later date, or the same position, or when using any other England Finder on any other microscope.

The location of the arrows is identical for all England Finder slides. Mark a label on the left hand side of the specimen slide, indicating the orientation to be repeated. By replacing the specimen slide with England Finder, taking care not to disturb the position, feature of interest can be noted. The feature can also be re-located at another place or time by reversing the procedure. A total of 100 positions on a slide can be accurately located.

Pattern	Description	Catalog	Price
S7	The England Finder	68048-07	250.00

Makler Counting Chamber

For Rapid Semen Analysis – The Makler counting chamber is only 10 microns deep, which is one tenth the depth of other hemacytometers., making it the shallowest of known chambers. This quality chamber is constructed from two pieces of optically flat glass: the first is the chamber, the second is the cover glass, which has a fine grid pattern of 1mm squares and a center area further subdivided into 0.1mm squares. Four quartz pins of precise height hold the cover glass to give an exact trapped specimen depth of 10 microns.

Catalog	Description	Price
68052-01	Makler Counting Chamber	1,395.00

Combination Eyepiece Graticule and Stage Micrometer Sets

We have now simplified the way in which you order The Eyepiece Graticule/Stage Micrometer Sets to suit your application, Simply select the objective magnification on the microscope, and then look in the next column to find the measurement size range that suits you best. You then find the set order reference and the description for your calibration set.
Please state the eyepiece graticule diameter on your order.

Combinations Without Certification
Catalog #	Obj. Mag.	Size Range	Eyepiece Graticule	Stage (Object) Micrometer	Price
For Transmitted Light
68060-05A	0.5	0.1 to 10mm	NE5, 5mm in 0.05mm	S1, 10mm in 0.1mm	325.00
68060-05B	0.5	0.01 to 1mm	NE28, 1mm in 0.01mm	S8, 1mm in 0.01mm	350.00
68060-1A	1	0.1 to 10mm	NE1, 10mm in 0.1mm	S1, 10mm in 0.1mm	310.00
68060-1B	1	0.01 to 1mm	NE28, 1mm in 0.01mm	S8, 1mm in 0.01mm	425.00
68060-2A	2	0.1 to 10mm	NE5, 5mm in 0.05mm	S1, 10mm in 0.1mm	310.00
68060-2B	2	0.01 to 1mm	NE5, 5mm in 0.05mm	S8, 1mm in 0.01mm	250.00
68060-4	4	0.01 to 1mm	NE5, 5mm in 0.05mm	S8, 1mm in 0.01mm	310.00
68060-10A	10	0.01 to 1mm	NE1, 10mm in 0.1mm	S8, 1mm in 0.01mm	370.00
68060-10B	10	0.002 to 0.1mm	NE28, 1mm in 0.01mm	S12, 0.1mm in 0.02mm	425.00
68060-16A	16	0.01 to 1mm	NE1, 10mm in 0.1mm	S8, 1mm in 0.01mm	310.00
68060-16B	16	0.002 to 0.1mm	NE5, 5mm in 0.05mm	S12, 0.1mm in 0.02mm	425.00
68060-20A	20	0.01 to 1mm	NE1, 10mm in 0.1mm	S8, 1mm in 0.01mm	310.00
68060-20B	20	0.002 to 0.1mm	NE5, 5mm in 0.05mm	S12, 0.1mm in 0.02mm	425.00
68060-40A	40	0.01 to 1mm	NE1, 10mm in 0.1mm	S8, 1mm in 0.01mm	310.00
68060-40B	40	0.002 to 0.1mm	NE5, 5mm in 0.05mm	S12, 0.1mm in 0.02mm	425.00
68060-60	60	0.002 to 0.1mm	NE1, 10mm in 0.1mm	S12, 0.1mm in 0.02mm	425.00
68060-100	100	0.002 to 0.1mm	NE1, 10mm in 0.1mm	S12, 0.1mm in 0.02mm	425.00
For Reflected Light
68062-10	10	0.1 to 1mm	NE1, 10mm in 0.1mm	S78, 1mm in 0.01mm	320.00

 

Combinations With UKAS Certification
Catalog #	Obj. Mag.	Size Range	Eyepiece Graticule	Stage (Object) Micrometer	Price
For Transmitted Light
68061-05A	0.5	0.1 to 10mm	NE5, 5mm in 0.05mm	S1, 10mm in 0.1mm	475.00
68061-05B	0.5	0.01 to 1mm	NE28, 1mm in 0.01mm	S8, 1mm in 0.01mm	500.00
68061-1A	1	0.1 to 10mm	NE1, 10mm in 0.1mm	S1, 10mm in 0.1mm	475.00
68061-1B	1	0.01 to 1mm	NE28, 1mm in 0.01mm	S8, 1mm in 0.01mm	490.00
68061-2A	2	0.1 to 10mm	NE5, 5mm in 0.05mm	S1, 10mm in 0.1mm	490.00
68061-2B	2	0.01 to 1mm	NE5, 5mm in 0.05mm	S8, 1mm in 0.01mm	490.00
68061-4	4	0.01 to 1mm	NE5, 5mm in 0.05mm	S8, 1mm in 0.01mm	480.00
68061-10A	10	0.01 to 1mm	NE1, 10mm in 0.1mm	S8, 1mm in 0.01mm	475.00
68061-10B	10	0.002 to 0.1mm	NE28, 1mm in 0.01mm	S12, 0.1mm in 0.02mm	800.00
68061-16A	16	0.01 to 1mm	NE1, 10mm in 0.1mm	S8, 1mm in 0.01mm	475.00
68061-16B	16	0.002 to 0.1mm	NE5, 5mm in 0.05mm	S12, 0.1mm in 0.02mm	800.00
68061-20A	20	0.01 to 1mm	NE1, 10mm in 0.1mm	S8, 1mm in 0.01mm	475.00
68061-20B	20	0.002 to 0.1mm	NE5, 5mm in 0.05mm	S12, 0.1mm in 0.02mm	800.00
68061-40A	40	0.01 to 1mm	NE1, 10mm in 0.1mm	S8, 1mm in 0.01mm	480.00
68061-40B	40	0.002 to 0.1mm	NE5, 5mm in 0.05mm	S12, 0.1mm in 0.02mm	800.00
68061-60	60	0.002 to 0.1mm	NE1, 10mm in 0.1mm	S12, 0.1mm in 0.02mm	800.00
68061-100	100	0.002 to 0.1mm	NE1, 10mm in 0.1mm	S12, 0.1mm in 0.02mm	800.00
For Reflected Light
68063-10	10	0.1 to 1mm	NE1, 10mm in 0.1mm