Electron Microscopy Sciences

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Illuminators, Magnifiers, Microscopes & Graticules

Electron Microscopy Sciences

arrow14Stage Graticules

These stage graticules are intended for the routine calibration of eyepiece patterns, particularly when alternating between objectives on one microscope or when using the same graticule in different microscopes. Their robust construction makes them ideal for student use and for instructional purposes. The scale or grid is centered on a glass disc mounted in a black anodized aluminum slide 75mm x 24mm x 2mm thick. For applications requiring traceable standards, see page 507.

Two types are available:

arrow12 A. SCALES AND MICROMETER

arrow12 For Transmitted Light

standard and crossed patternsarrow10 Standard and Crossed Patterns - S1, S2, S4, S5, S8, S11, S12, S16, S22, S48

Pattern
Type, Length
Nos. Div.
Div. Size
Catalog Price  
S1
Horizontal, 10mm
100
0.1mm
68040-01 192.00 cart
S2
Horizontal, 5.0mm
100
0.05mm
68039-02 222.00 cart
S4
Horizontal, 0.1"
100
0.001"
68039-04 222.00 cart
S5
Horizontal, 20mm
200
0.01 mm
68039-05 308.00 cart
S8
Horizontal, 1.0mm
100
0.01mm
68042-08 192.00 cart
S11
Horizontal, 0.005"
50
0.0001"
68039-11 310.00 cart
S12
Horizontal, 0.1mm
50
0.002mm
68044-12 310.00 cart
S16
Crossed Scale, 1.0mm
100
0.01mm
68039-16 260.00 cart
S22
Vertical Scale, 2.0mm
200
0.01mm
68039-22 226.00 cart
S48
No Coverglass, 1.0mm
100
0.01mm
68041-48 220.00 cart

combined metric/imperial patternarrow10 Combined Metric/Imperial Pattern

Pattern Description
Catalog
Price  
S20 Double micrometer scale 2mm in 0.01mm divisions and 0.1" in 0.005" divisions.
68043-20
295.00 cart

grouped graduation patternarrow10 Grouped Graduation Pattern - S21

For speedy determination of a range of featured sizes within a given specimen.

Pattern Description
Catalog
Price  
S21 Micrometer scale 5mm in 0.5mm divisions, 2mm in 0.1mm divisions, and 0.2mm in 0.01mm divisions.
68043-21
208.00 cart

Counting Slide 0.01mm squaresgridsarrow10 Grids - S9, S10, S28, S29

Pattern Description
Catalog
Price
S9 Counting Slide 0.1mm squares.
68045-09
220.00
cart
S10 Counting Slide 0.05mm squares .
68045-10
220.00
cart
S28 0.01mm grid / 0.2 x 0.2mm overall.
68045-28
240.00
cart
S29 0.01mm grid / 1.5 x 1.5mm overall.
68045-29
240.00
cart

arrow10 Diamond Ruled Stage Micrometer - S91

A stage micrometer with very well defined lines. These reference scales offer substantial improvements in definition over the standard types when used under the highest magnification. This is made possible by the use of diamond cutting tools and a special ruling engine.
The S91 has clear lines ruled through a semi opaque metal film, which with transmitted light, appear as bright lines on a dark background.
The micrometer is made on a glass substrate 76mm x 26mm x 1.2mm thick. The lines are 1 micron wide or less and 3.5mm long. The metric rulings provide a scale of 1mm divided into 0.1mm parts, with one part being sub-divided into 0.001mm parts.

Pattern Description
Catalog
Price  
S91 Diamond ruled stage micrometer
68046-91
300.00 cart

reflected lightarrow12 For Reflected Light

This scale is etched through highly reflective vacuum coated metal. When viewed under vertical illumination, as with a metallurgical microscope, the scale appears black against a bright background.

arrow10 Reflected Light - S78

Pattern Description
Catalog
Price  
S78 Micrometer scale 1mm in 0.01mm divisions.
68043-78
208.00 cart

arrow12 B. STAGE CALIBRATION STANDARDS

These stage micrometers differ from the standard stage micrometers due to the fact they are being available with a certificate giving precise details about their accuracy. These calbrated standards provide traceability for the precise calibration and confirmation of accuracy of optical measuring instruments, which is necessary under ISO provisions.

micrometer standardsarrow10Micrometer Standards - PS1, PS4, PS5, PS8, PS12, PS16, PS78

These standards are differ from the stage micrometers listed on page 504. The glass discs are mounted in stainless steel slides with engraved serial numbers. Each slide is supplied in a polished wooden storage case to distinguish it as a traceable standard of high value. We can arrange for the calibration of its scales and grids to be carried out by the most appropriate laboratory to suit the customers requirements – the choice of laboratory is normally dependent on the nature of the calibration and the accuracy required.

  1. Calibration by NPL: The National Physical Laboratory carries out measurements at selected points on the scale and grids and issues a certificate of calibration. Order Code NP. The NPL is the English equivalent of NIST.
  2. Calibration by UKAS accredited laboratory: A UKAS accredited laboratory carries out measurements at selected points on the scales and grids and issues a calibration certificate. Order Code: NA
  3. Measurement by Us: For applications, which do not require the accuracy provided by calibrations carried out by NPL or a UKAS accredited laboratory, We can provide a Certificate of Comparison. The scale or grid is compared with NPL calibrated in-house standards and a statement is provided on the accuracy of the item with respect to these standards.
    Order Code GR
Pattern Description Catalog # Pack Price  
PS1 S1 with Graticules Certificate 68047-1GR   600.00 cart
  S1 with UKAS Certificate 68047-1NA   600.00 cart
  S1 with NPL Certificate 68047-1NP each 1195.00 cart
PS4 S4 with Graticules Certificate 68047-4GR   595.00 cart
  S4 with UKAS Certificate 68047-4NA   610.00 cart
  S4 with NPL Certificate 68047-4NP each 1225.00 cart
PS5 S5 with Graticules Certificate 68047-5GR   675.00 cart
  S5 with UKAS Certificate 68047-5NA   750.00 cart
  S5 with NPL Certificate 68047-5NP each 1300.00 cart
PS8 S8 with Graticules Certificate 68047-8GR   560.00 cart
  S8 with UKAS Certificate 68047-8NA   620.00 cart
  S8 with NPL Certificate 68047-8NP each 1200.00 cart
PS12 S12 with Graticules Certificate 68047-12GR   680.00 cart
  S12 with UKAS Certificate 68047-12NA   1200.00 cart
  S12 with NPL Certificate 68047-12NP each 1350.00 cart
PS16 S16 with Graticules Certificate 68047-16GR   675.00 cart
  S16 with UKAS Certificate 68047-16NA   850.00 cart
  S16 with NPL Certificate 68047-16NP each 1600.00 cart
PS78 S78 with Graticules Certificate 68047-78GR   620.00 cart
  S78 with UKAS Certificate 68047-78NA   625.00 cart
  S78 with NPL Certificate 68047-78NP each 1250.00 cart
Certification Price  
68047-C
NAMAS Certification for SI - S70 400.00 cart
68047-Cert
NPL Certification for SI - S70 1,000.00 cart

image analysis standardarrow10 Image Analysis Standard (Reference Stage Graticule)

This high precision image analysis standard provides four test areas designed for calibrating image analysis systems and identifying deviations and distortions in optical imaging systems.
The standard, which can also be used as a high precision stage micrometer, is supplied with recommendations for its use and an individual certificate of calibration.
It is produced on a 75mm x 25mm slide and has a square grid accuracy of ±0.1µm and a dot accuracy of ±0.3µm (except for the smallest and largest two dots on the root-2 array, where accuracy is ±0.5µm). The four test areas are:

Catalog
Description Price  
68049
Reference stage graticule 75mm x 25mm slide. 2700.00 cart

test slide for phase contrastarrow10 H.S.E./N.P.L. Test Slide for Phase Contrast Microscopy - S84

This test slide is made in the UK and is under license from the National Physical Laboratory. It is an epoxy replica of a master slide produced and certified by that laboratory. The replicas are mounted on microscope slides of 1.2mm thickness with cover glass of 0.17mm thickness. This information is transferred to the accompanying table, which indicates the maximum phase change passing through test objects to test slide. A satisfactory system will detect block 5. Full details are supplied with the slide.

Pattern Description Catalog Price  
S84 HSE Test Slide for Calibration in Asbestos Analysis 68038-84H 1,380.00 cart

arrow12 C. VIBRATION

foepple dot vibration testarrow10 FOE PPL Dot - S25

The pair of dots, which appear to merge into a single dot, determines the amount of vibration of the slide in the appropriate axis. The pattern on the S25 is an array of 20 pairs of dots converging on a single dot. The distance between each dot pair increases by 0.001 inch to a maximum of 0.02 inch, pairs being squispaced 0.25 inch.

Pattern Description Catalog Price  
S25 Foepple Dot Vibration Test 68038-25 280.00 cart

arrow12 D. VOLUME/COUNTING CELL

These are used to create precise volumes of liquid to enable counting of particles in a specified volume of liquid. A thick slide (glass or plastic) has an area cut into its surface that will hold precise volumes of bathing fluid. The shallow well, facilitating easy counting of contained particles. In use, a single drop of liquid is placed on the slide and a coverglass is placed on the platform providing equal and accurate subdivisions of the fluid each of which can be observed for particle counting.

sedgewick rafter and cover glassarrow10 Sedgewick Rafter and Cover Glass - S50, S51, S52

For use in water analysis, culture inspection, and any fluid where particles per unit volume need to be determined. Available in two versions:
S50 is an economically priced plastic cell.
S52 is made from glass, with a chromium surface image. This is intended for professional use and when using phase contrast. In each type, a central cell is filled with fluid and a coverglass limits the volume to 1 milliliter. The grid subdivides this into micro-liters.
S51 is the spare cover glass for S50 and S52. Each cell is supplied with one cover glass.

Pattern Description Catalog #   Price  
S50 S50, Sedgewick Rafter, Plastic 68050-50 each 56.00 cart
S52 S52, Sedgewick Rafter, Glass 68050-52 each 250.00 cart
S51 S51, Cover Glass for S50 & S52 68050-51 each 16.00 cart

howard cell and cover glassarrow10 Howard Cell and Cover Glass - S60, K20

The Howard cell is a glass slide 76mm x 35mm with a central circular island and is used for counting mold fibers and spores in fruit juices, especially from tomatoes. With the K20 coverglass in place a 0.1mm thickness of liquid is contained over the central island. The coverglass has 25 calibrated fields of 1.382mm diameter through which to view the particles. This coverglass removes the necessity of precise adjustment of the microscope and eyepiece graticule in the original Howard method.

Pattern Description Catalog # Price  
S60 Howard Cell for Fruit Juices 68051-60 302.00 cart
K20 Sedgewick Rafter Counting Chamber (glass) 68051-20 92.00 cart

arrow12 E. FINDER GRATICULES

Finder graticules are used to swiftly and accurately give a position of reference to an area of interest on a specimen.

the england finderarrow10 The England Finder – S7

S7 is a glass slide 75mm x 26mm (3x1") marked over the top surface in a way that a referenced position can be directly read relative to the locating edges. That means it is marked with a square grid at 1mm intervals. Each square contains a center ring bearing reference letters and numbers, the remainder of the square being subdivided into four segments numbered 1 to 4.

Reference numbers run horizontally 1 to 75 and letters vertically A to Z (omitting I) The main locating edge is the bottom of the slide, which is used in conjunction with either the left or right vertical edge of the slide.

All England Finders produced for over 40 years are identical. The purpose of the finder is to give the microscopist an easy method of recording the position of a particular field of interest, so that another person in another laboratory can relocate at a later date, or the same position, or when using any other England Finder on any other microscope.

The location of the arrows is identical for all England Finder slides. Mark a label on the left hand side of the specimen slide, indicating the orientation to be repeated. By replacing the specimen slide with England Finder, taking care not to disturb the position, feature of interest can be noted. The feature can also be re-located at another place or time by reversing the procedure. A total of 100 positions on a slide can be accurately located.

Pattern Description Catalog Price  
S7 The England Finder 68048-07 250.00 cart

makler counting chambermakler counting chambersarrow10 Makler Counting Chamber

For Rapid Semen Analysis – The Makler counting chamber is only 10 microns deep, which is one tenth the depth of other hemacytometers., making it the shallowest of known chambers. This quality chamber is constructed from two pieces of optically flat glass: the first is the chamber, the second is the cover glass, which has a fine grid pattern of 1mm squares and a center area further subdivided into 0.1mm squares. Four quartz pins of precise height hold the cover glass to give an exact trapped specimen depth of 10 microns.

Catalog Description Price  
68052-01 Makler Counting Chamber 1,395.00 cart

arrow12 Combination Eyepiece Graticule and Stage Micrometer Sets

We have now simplified the way in which you order The Eyepiece Graticule/Stage Micrometer Sets to suit your application, Simply select the objective magnification on the microscope, and then look in the next column to find the measurement size range that suits you best. You then find the set order reference and the description for your calibration set.
Please state the eyepiece graticule diameter on your order.

Combinations Without Certification
Catalog #
Obj.
Mag.
Size
Range
Eyepiece
Graticule
Stage (Object)
Micrometer
Price
 
For Transmitted Light
68060-05A
0.5
0.1 to 10mm
NE5, 5mm in 0.05mm
S1, 10mm in 0.1mm
325.00
cart
68060-05B
0.5
0.01 to 1mm
NE28, 1mm in 0.01mm
S8, 1mm in 0.01mm
350.00
cart
68060-1A
1
0.1 to 10mm
NE1, 10mm in 0.1mm
S1, 10mm in 0.1mm
310.00
cart
68060-1B
1
0.01 to 1mm
NE28, 1mm in 0.01mm
S8, 1mm in 0.01mm
425.00
cart
68060-2A
2
0.1 to 10mm
NE5, 5mm in 0.05mm
S1, 10mm in 0.1mm
310.00
cart
68060-2B
2
0.01 to 1mm
NE5, 5mm in 0.05mm
S8, 1mm in 0.01mm
250.00
cart
68060-4
4
0.01 to 1mm
NE5, 5mm in 0.05mm
S8, 1mm in 0.01mm
310.00
cart
68060-10A
10
0.01 to 1mm
NE1, 10mm in 0.1mm
S8, 1mm in 0.01mm
370.00
cart
68060-10B
10
0.002 to 0.1mm
NE28, 1mm in 0.01mm
S12, 0.1mm in 0.02mm
425.00
cart
68060-16A
16
0.01 to 1mm
NE1, 10mm in 0.1mm
S8, 1mm in 0.01mm
310.00
cart
68060-16B
16
0.002 to 0.1mm
NE5, 5mm in 0.05mm
S12, 0.1mm in 0.02mm
425.00
cart
68060-20A
20
0.01 to 1mm
NE1, 10mm in 0.1mm
S8, 1mm in 0.01mm
310.00
cart
68060-20B
20
0.002 to 0.1mm
NE5, 5mm in 0.05mm
S12, 0.1mm in 0.02mm
425.00
cart
68060-40A
40
0.01 to 1mm
NE1, 10mm in 0.1mm
S8, 1mm in 0.01mm
310.00
cart
68060-40B
40
0.002 to 0.1mm
NE5, 5mm in 0.05mm
S12, 0.1mm in 0.02mm
425.00
cart
68060-60
60
0.002 to 0.1mm
NE1, 10mm in 0.1mm
S12, 0.1mm in 0.02mm
425.00
cart
68060-100
100
0.002 to 0.1mm
NE1, 10mm in 0.1mm
S12, 0.1mm in 0.02mm
425.00
cart
For Reflected Light
68062-10
10
0.1 to 1mm
NE1, 10mm in 0.1mm
S78, 1mm in 0.01mm
320.00
cart

 

Combinations With UKAS Certification
Catalog #
Obj.
Mag.
Size
Range
Eyepiece
Graticule
Stage (Object)
Micrometer
Price
 
For Transmitted Light
68061-05A
0.5
0.1 to 10mm
NE5, 5mm in 0.05mm
S1, 10mm in 0.1mm
475.00
cart
68061-05B
0.5
0.01 to 1mm
NE28, 1mm in 0.01mm
S8, 1mm in 0.01mm
500.00
cart
68061-1A
1
0.1 to 10mm
NE1, 10mm in 0.1mm
S1, 10mm in 0.1mm
475.00
cart
68061-1B
1
0.01 to 1mm
NE28, 1mm in 0.01mm
S8, 1mm in 0.01mm
490.00
cart
68061-2A
2
0.1 to 10mm
NE5, 5mm in 0.05mm
S1, 10mm in 0.1mm
490.00
cart
68061-2B
2
0.01 to 1mm
NE5, 5mm in 0.05mm
S8, 1mm in 0.01mm
490.00
cart
68061-4
4
0.01 to 1mm
NE5, 5mm in 0.05mm
S8, 1mm in 0.01mm
480.00
cart
68061-10A
10
0.01 to 1mm
NE1, 10mm in 0.1mm
S8, 1mm in 0.01mm
475.00
cart
68061-10B
10
0.002 to 0.1mm
NE28, 1mm in 0.01mm
S12, 0.1mm in 0.02mm
800.00
cart
68061-16A
16
0.01 to 1mm
NE1, 10mm in 0.1mm
S8, 1mm in 0.01mm
475.00
cart
68061-16B
16
0.002 to 0.1mm
NE5, 5mm in 0.05mm
S12, 0.1mm in 0.02mm
800.00
cart
68061-20A
20
0.01 to 1mm
NE1, 10mm in 0.1mm
S8, 1mm in 0.01mm
475.00
cart
68061-20B
20
0.002 to 0.1mm
NE5, 5mm in 0.05mm
S12, 0.1mm in 0.02mm
800.00
cart
68061-40A
40
0.01 to 1mm
NE1, 10mm in 0.1mm
S8, 1mm in 0.01mm
480.00
cart
68061-40B
40
0.002 to 0.1mm
NE5, 5mm in 0.05mm
S12, 0.1mm in 0.02mm
800.00
cart
68061-60
60
0.002 to 0.1mm
NE1, 10mm in 0.1mm
S12, 0.1mm in 0.02mm
800.00
cart
68061-100
100
0.002 to 0.1mm
NE1, 10mm in 0.1mm
S12, 0.1mm in 0.02mm
800.00
cart
For Reflected Light
68063-10
10
0.1 to 1mm
NE1, 10mm in 0.1mm