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Electron Microscopy Sciences

Specimen Preparation and Embedding Supplies

Downloadable PDFarrow13ACLAR®–Fluoropolymer Films

msdsarrow12An Embedding/Cell Growing Film

ACLAR® is made from fluorinated-chlorinated resins. There are four basic film types–the homopolymer ACLAR® Rx Series, the copolymers ACLAR® 22A and 33C and the new ACLAR® Cx. (Honeywell/Allied Signal). The chemical make-up of all ACLAR® products provide an exceptional moisture barrier. ACLAR® is crystal clear, biochemically inert, highly resistant to most chemicals and sterilizable by heat or radiation. ACLAR® is used widely in pharmaceutical, medical, sensitive electronics and military packaging.

ACLAR® 33C is a copolymer film consisting primarily of chlorotrifluoroethylene (CTFE). It offers an outstanding moisture barrier, excellent chemical resistance and minimal dimensional change (<2%), making it the best choice for use in microscopy.

Aclar® UltRx 2000 is a 2.00 mil (51 micron) PCTFE homopolymer, high performance barrier film for the pharmaceutical and medical markets. It thermoforms well on conventional blister packaging equipment and provides the best barrier of any clear film.

Features:

  • Crystal clear, high UV transparency– ideal for use in UV curing of embedding resin in microscopy.
  • Chemically stable and biochemical\y inert– the product of choice for growing cell cultures.
  • Low dielectric constant, high electric strength and dissipation factor– offers excellent cell attachment even through lengthy processing procedures.
  • Low surface energy–and separates easily from epoxy.
  • Exhibits no detectable autofluorescence– ideal for fluorescent microscopy.
  • A non-flammable, non-aging co-polymer– which is suited for sterilization by heat or UV.
  • Crystalline melting point is 206°C (403°F)– stable in the SEM.
  • Flexible and soft– can be sectioned without damage to the ultramicrotome knives.
arrow12ACLAR® 33C Film

ACLAR® 33C film is now available in 7.8 mil (199µm) thickness and it is offered in a 8” x 10” sheet form with package quantities of 10 or 25.

50425-10 ACLAR® 33C Film, 8”x 10”, 7.8 Mil 10 80.00 Add to Cart
50425-25 ACLAR® 33C Film, 8”x 10”, 7.8 Mil 25 182.00 Add to Cart

ACLAR® 33C Physical Data Sheet

(These are only typical values and are not to be interpreted as product specifications):

Typical Value
Properties@ 73°F ~50% RH English Metric Test Method
Gravity Specific 2.12 ASTM D1505
Yield 7.8 mil 1,677 in2/lb. 2.38m2/kg  
Haze <4.5% ASTM D1003
Crystalline Melting Point 403°F 206°C ASTM D4591
Dimensional Stability, 10 min @ 300°F
-MD
-TD
<2%
<2%
ASTM D1204
Tensile Strength
-MD
-TD
3000-4600 psi
3000-4000 psi
21-32 MPa
21-38 MPa
ASTM D882
Elongation (MD/TD) 50-125% ASTM D882
Modulus, Secant (MD/TD) 185,000-200,000 psi 1276-1379 MPa ASTM D882
Tear Strength, Graves (MD/TD) 425-525 g/mil ASTM D1004
Water Vapor Transmission
Rate @ 100°F/ 100% RH
0.003 gm/100 in2/day 0.047 gm/m2/day ASTM F1249
Thermal Conductivity 4.7 x 10-4 cal-cm/cm2sec °C  
Flammability Nonflammable  
Oxygen Index 100 ASTD D2683
arrow12ACLAR® UltRx 2000 Film

ACLAR® UltRx 2000 film is available in 2 (51µm) mil thickness and it is offered in a 11.69” x 8.27” sheet form with package quantities of 10 or 25.

50426-10 ACLAR® UltRx 2000 Film, 11.69”x 8.27”, 2 Mil 10 215.00 Add to Cart
50426-25 ACLAR® UltRx 2000 Film, 11.69”x 8.27”, 2 Mil 25 485.00 Add to Cart

ACLAR® UltRx 2000 Physical Data Sheet

(These are only typical values and are not to be interpreted as product specifications):

Typical Value
Properties@ 73°F ~50% RH English Metric Test Method
Gravity Specific 2.11 ASTM D1505
Yield 2.0 mil 6,567 in2/lb. 9.34 m2/kg  
Haze <1% ASTM D1003
Crystalline Melting Point 412°F 211°C ASTM D4591
Dimensional Stability, 10 min @ 300°F
-MD
-TD
<+6%
<-6%
ASTM D1204
Tensile Strength
-MD
-TD
7,000-10,000 psi
4,500-7,500 psi
48-69 MPa
31-52 MPa
ASTM D882
Elongation (MD/TD) 150-200%
175-250%
ASTM D882
Modulus, Secant (MD/TD) 170,000-200,000 psi 1276-1379 MPa ASTM D882
Surface Tension (Treated Side) ≥42 dynes/cm  
Water Vapor Transmission
Rate:
gm/100 in2/day gm/m2/day ASTM F1249
@ 77°F (25°C)/60% RH 0.0012 0.0186  
@ 86°F (30°C)/60% RH 0.0025 0.0388  
@ 104°F(40°C)/75% RH 0.0066 0.102  
@ 100°F(37.8°C)/100% RH 0.0077 0.119  

ACLAR® Chemical Data Sheet

Chemicals Average Weight Increase Visible Effect on Sample
Acetone 0.5% None
Butyl Alcohol - None
Carbon Tetrachloride 1.6% Slightly flexible
1,2-Dichloroethane 0.03% None
Ethyl Acetate 6.0% Very flexible
Ethyl Alcohol None None
Ethyl Ether 5.2% Very flexible
Ethylene Oxide 4.0% Very flexible
Formic Acid None None
Gasoline None None
All Acids (HCl, H2SO4..) None None
Methanol None None
Propylene Oxide - None
Toluene 1.1 Slightly flexible
Osmium Tetroxide None None
Plastisolve - None

Kingsley R.E, Cole N. L.: Preparation of cultured mammalian cells for transmission and scanning electron microscopy using ACLAR® film. Journal of Electron Microscopy Technique, 10, 77-85 (1988)

Mauve G.M., Brenham J.C., Beattie, M.S.: Ultrastructure of HRP-labeled neurons: a comparison of two sensitive techniques. Brain Research Bulletin, 10, 551 (1983)

Masurovsky E.B., Bunge R.P.: Aclar Film in Biological Electron Microscopy. Journal of Electron Microscopy Technique, 12, 172-173 (1989)

Masurovsky E.B., Bunge R.P.: Fluoroplastic coverslips for long-term nerve tissue culture. Stain Technology, 43, 3, 161–165 (1968)

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