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Electron Microscopy Sciences

Specimen Preparation and Embedding Supplies

arrow13Cell Culture: Coverglass

CultureWell™ Removable Chambered CoverglassCultureWell Removable Chambered Coverglassarrow11CultureWell™ Removable Chambered Coverglass

CultureWell™ with removable chambered coverglass – ideal for cell culture and fluorescence imaging applications

The 2 x 8, 16 chambered coverglass is like a chambered slide with the imaging advantages of a removable coverglass substructure. It is designed with standard 96 well plate volumes and well spacing. The non-cytotoxic silicone well gasket forms a leak-proof seal between the polystyrene upper structure and the coverglass. CultureWell™ Chambered Coverglass is shipped sterile and ready to use.

Features:

  • The growth surface is No. 1.5 German borosilicate coverglass.
  • Inert, non-cytotoxic silicone permits complete edge-to-edge growth of cells.
  • Well spacing allows use of multi-channel pipettes for ease and speed of handling
  • Cell growth characteristics are optimal due to the use of inert silicone materials and manufacturing process.
  • Quality test for non-leakage, cell growth and coverglass removal to ensure consistent results
  • Silicone well gasket remains attached to coverglass after separation, allowing wells to be used as reagent reservoirs
  • Gasket well design is ideally suited for small volume incubations, in-situ hybridization and immunostaining
  • Black well gasket reduces light scatter, enhancing fluorescence applications. The gasket can easily be removed from the coverglass for mounting
  • Frosted microscope slides are provided for mounting

Key product dimensions include:

  • Coverslip gasket thickness is 0.5 mm
  • Interior diameter of each well is approx. 6 mm
  • Volume per gasket well (upper structure removed) is approx. 9 µl
  • Volume per well with the upper structure is 250 µl

Ordering:

EMS # Qty Price Select Specification
70459-08 8/pk 200.00 Add to Cart CultureWell Removable Chambered Coverglass Specifications
70459-08-T each 9.95 Add to Cart

16 Well CultureWell™ Removeable Chambered Coverglass
No 1.5 Desag Coverglass; Sterile
Culture area per well 0.4 cm2

Spare CultureWell™ Coverglass Removal Tool,
Order Separately (70459-08-T)
CultureWell Coverglass Removal Tool

CultureWell™ Chambered Coverglass CultureWell Chambered Coverglassarrow11CultureWell™ Chambered Coverglass

Chambered coverglass products are optimal for high resolution microscopy

Chambered coverglass products consists of removable / reusable non-cytotoxic silicone gaskets secured to 24 mm x 50 mm, #1.5 German coverglass. They may be placed in sterile culture plates or 100 mm diameter culture dishes.

Features:

  • The thin flat coverglass is the optimal platform for high resolution microscopy and high-sensitivity, quantitative, fluorescent imaging
  • Leak-proof design eliminates well-to-well cross contamination
  • Gaskets stabilize coverglass and provide convenient handling using conventional microscope slide holders and accessories
  • Gaskets are removable for high resolution imaging
  • Gasket may be re-sterilized and reused
  • Well spacing facilitates liquid handling utilizing multichannel pipettes and robotic dispensers.

Ordering:

EMS # Depth Price Select Specification
70460-2R1 1mm 198.00 Add to Cart CultureWell Chambered Coverglass Specifications
70460-2R2 1.5mm 219.00 Add to Cart
2 Wells – 15mm Dia
250-400µl Volume (-2R1)
300-500µl Volume (-2R2)
20 per pack



70460-3S 1mm 198.00 Add to Cart CultureWell Chambered Coverglass Specifications
3 Wells – 9.5mm x 9.5mm ID
300-500µ Volume
20 per pack




70460-4R 1mm 198.00 Add to Cart CultureWell Chambered Coverglass Specifications
4 Wells – 9mm Dia
50-100µl Volume
20 per pack





70460-50R 1mm 198.00 Add to Cart CultureWell Chambered Coverglass Specifications
50 Wells – 3mm Dia
3-10µl Volume
20 per pack




70460-8R 1mm 198.00 Add to Cart CultureWell Chambered Coverglass Specifications
8 Wells – 6mm Dia
15-30µl Volume
20 per pack




CultureWell™ Chambered Coverglass InsertsCultureWell Chambered Coverglass Insertsarrow11CultureWell™ Chambered Coverglass Inserts

Each CultureWell™ insert is comprised of four chambered coverglass assembled in a disposable frame which is placed in a standard 86 mm x 128 mm culture plate. All are sterile and ready to use.

The stabilizing insert design and accessory hardware allow fragile coverglass to be easily integrated into protocols for high throughput handling of microscope slides and SBS compliant microtiter plates.

Ordering:

EMS # Wells Dia Volume Depth Qty Price Select
70461-2R1 8 6mm 300-500µ 1mm 10/pk 648.00 Add to Cart
70461-2R2 8 6mm 300-500µ 1.5mm 10/pk 760.00 Add to Cart
CultureWell Chambered Coverglass Insert Specifications
70461-3S 3 Wells 9.5mm x 9.5mm ID 300-500µ 1mm 10/pk 760.00 Add to Cart
CultureWell Chambered Coverglass Insert Specifications
70461-4R 4 Wells 9mm 50-100µ 1mm 10/pk 995.00 Add to Cart
CultureWell Chambered Coverglass Insert Specifications
70461-50R 50 Wells 3mm 3-10µl 1mm 10/pk 995.00 Add to Cart
CultureWell Chambered Coverglass Insert Specifications
70461-8R 8 Wells 6mm 15-30µl 1mm 10/pk 995.00 Add to Cart
CultureWell Chambered Coverglass Insert Specifications

SecureSlip Cell Culture Coverglassarrow11SecureSlip™ Cell Culture Coverglass

Convenient, Secure coverglass that will not move during cultivation

The coverglass is affixed to a thin microscopically transparent silicone base which secure to culture vessels by an electrostatic charge, not an adhesive, to prevent movement during cultivation.

The silicone also acts as a hydrophobic barrier to isolate liquid reagents on glass. Individual coverglass with silicone backing can be adhered to a petri dish or plate. These can be easily removed one at a time from the dish during culture. Once removed, coverglass can be affixed to a microscope slide by means of the tacky silicone backing for routine processing, or placed cell side down onto a drop of reagent using the silicone backing as a cover to prevent evaporation.

Features:

  • Silicone backing peels off easily for conventional mounting on microscope slides
  • During culture, cells may be imaged without interference from the optical grade silicone
  • Eliminates coverglass overlapping during culture. Handle SecureSlips™ with forceps by the silicone backing to avoid damage to glass or cells
  • Easily identify "cell side" of coverglass

Ordering:

70462-12 378.00 Add to Cart SecureSlip Cell Culture Coverglass Specifications
15 Well SecureSlip Silicone Supported Coverglass
12 mm x 12mm Coverglass
75 per pack








70462-14 378.00 Add to Cart SecureSlip Cell Culture Coverglass Specifications
15 Well SecureSlip Silicone Supported Coverglass
12 mm Dia Coverglass
75 per pack







MultiSlip™ Cell Culture Coverglass InsertsMultiSlip Cell Culture Coverglass Insertsarrow11MultiSlip™ Cell Culture Coverglass Inserts

Sterile, ready-to-use MultiSlip™ for culture of cells where pretreatment with a biological coating of glass surface is required.

MultiSlip™ insert with 8 (18mm x 18mm) or 15 (12mm x 12mm) number 1.5 thickness German coverglass per insert are sterile and ready to use in conventional 86mm x 128mm culture plates.

Staining and washing procedures may be performed with MultiSlip™ inserts in the plate, or silicone backed coverglass may be removed individually and affixed to glass microscope slides. Alternatively, inserts may be easily removed for batch processing in glass staining dishes.

MultiSlips™ are ideally suited for the culture of cells where pretreatment of glass surfaces with a biological coating is required. Simply add sterile solution to the plate incubates and aspirate. Coating is applied evenly to one side of the glass only, with no over-lapping, handling with forceps.

Ordering:

Diagram of MultiSlip Cell Culture Coverglass Insert
EMS # Specification Qty Price Select
70463-12 15 – 12mm x 12mm Silicone Supported Coverglass Per Tray 10/pk 660.00 Add to Cart
70463-18 8 – 18mm x 18mm Silicone Supported Coverglass Per Tray 10/pk 690.00 Add to Cart
Diagram of MultiSlip Cell Culture Coverglass Insert

arrow11CultureWell™ Accessories

Reusable CultureWell™ gaskets from CultureWell™ Chambered Coverglass and CultureWell™ Chamber Coverglass Inserts are available bulk packaged.

  • Gaskets are ideal for forming wells on glass microscope slides or in polystyrene dishes
  • Gaskets are non-sterile and may be sterilized by autoclaving, UV or alcohol.

Ordering:

EMS # Depth Price Select Specification
70465-2R1 1mm 72.00 Add to Cart CultureWell Gasket Specifications
70465-2R2 1.5mm 79.00 Add to Cart
Gasket Only
2 Wells – 15mm Dia
250-400µl Volume (-2R1)
300-500µl Volume (-2R2)
10 per pack



70465-3S 1mm 72.00 Add to Cart CultureWell Gasket Specifications
Gasket Only
3 Wells – 9.5mm x 9.5mm ID
300-500µ Volume
10 per pack



70465-4R 1mm 72.00 Add to Cart CultureWell Gasket Specifications
Gasket Only
4 Wells – 9mm Dia
50-100µl Volume
10 per pack


70465-8R 1mm 72.00 Add to Cart CultureWell Gasket Specifications
Gasket Only
8 Wells – 6mm Dia
15-30µl Volume
10 per pack


70465-50R 1mm 77.00 Add to Cart CultureWell Gasket Specifications
Gasket Only
50 Wells – 3mm Dia
3-10µl Volume
10 per pack


Additional Accessories:

EMS # Description Pack Price  
70466-10 86mm x 128mm Polystyrene Culture Plate, Sterile 10 31.00 Add to Cart
70466-20 Stainless Steel Handles for Inserts 5 55.00 Add to Cart
70466-30 24mm x 50mm, #1.5 Thickness German Coverglass 100 85.00 Add to Cart
70466-40 Reusable Silicone Well Dividers 2 - 18mm x 18mm Wells, 2.0mm Depth, Sterile 4 76.00 Add to Cart

Protein Crystallization Coversarrow11Protein Crystallization - Creative Tools for the Study of Cells

ProCrystal™ Protein Crystallization Covers: Hanging drop microtiter plate seals with unmatched drop formation and segregation and no noticeable x-ray diffraction

ProCrystal™ covers are designed for high throughput protein x-ray crystallography to generate atomic resolution structures of protein molecules1. They fit SBS compliant microtiter plates and are TTP Mosquito®*compatible. They are packed dust-free, ready-to-use in packs of 5 with either pre-cut or uncut seals.

The seals sit directly over the microplate crystallization wells. The seals are validated to be incorruptible and materials will not ingress into samples. They are available in either an individual well pre-cut format so that one can easily remove crystals using a forceps from individual wells without affecting neighboring wells or uncut format.

The plate seals are UV compatible with the highest optical quality available. They are exceptionally hydrophobic which maintains drop footprint and segregation even with the use of MPD, Glycerol or detergents.

Features:

  • Two Formats: Individually cut & single sheet un-cut seals
  • TTP LabTech Mosquito®* compatible
  • Ready-to-use, dust-free
  • UV Compatible
  • No noticeable X-ray diffraction
  • Accommodates up to 3 protein drops per well

References:

1Newby, et. al. A General Protocol for the Crystallization of Membrane Proteins for X-ray Structural Investigation. Nature Protocols, Vol. 4, 619-637 (April 1, 2009)

Ordering:

EMS # Type Price Select Specification
70470-10 Paper 54.00 Add to Cart ProCrystal Protein Crystallization Cover Specifications
70470-20 Plastic 57.00 Add to Cart
PCC Uncut Liner
5 per pack








70470-60 Paper 69.00 Add to Cart ProCrystal Protein Crystallization Cover Specifications
70470-70 Plastic 76.00 Add to Cart
PCC Cut Liner
5 per pack





References

CultureWell™ Products References

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