Lab-Tek® Chamber
Slide Products
Lab-Tek® Chamber Slide™ System: Lab-Tek® Chamber Slide™,
Lab-Tek® II Chamber Slide™ and Lab-Tek® II CC2™ Chamber
Slide™ Products
The growth of various cell types on plastic, soda lime glass and borosilicate coverglass were examined. The surfaces were either unmodified, coated with polylysine, or stably surface modified with non-biological reagents as described by Kleinfield, et al. (1988). All substrates were assembled into Lab-Tek® and Lab-Tek®II Chamber Slide™ products.
| Product Features | Lab-Tek® | Lab-Tek® II | Lab-Tek® II CC2 |
| Configurations | 1, 2, 4, 8, 16 well, flask style | 1, 2, 4, 8 well | 1, 2, 4, 8 well |
| Media chamber material | Proprietary cell culture grade polystyrene (PS) | Proprietary cell culture grade polystyrene (PS) | Proprietary cell culture grade polystyrene (PS) |
| Slide Materials | Soda lime glass Permanox® plastic Polystyrene plastic |
Soda lime glass | Soda lime glass |
| Cover | Clear polystyrene, no handle, facilitates microscopic viewing | Clear polystyrene, with handle for aseptic manipulations | Clear polystyrene, with handle for aseptic manipulations |
| Sealant | Non-toxic medical grade silicone • Remains on slide after detachment |
Biocompatible acrylic adhesive |
Biocompatible acrylic adhesive • Remains with media chamber after detachment |
| Reagent ‘isolator’ | Created by removable silicone gasket |
Created by hydrophobic border | Created by hydrophobic border |
Chamber separation |
No separation tool required | Requires separation tools | Requires separation tools |
| Growth Surface | For adherent cell culture Glass slides • 6-stage high purity water wash Permanox® plastic • Cell culture treated Polystyrene • Cell culture treated |
For adherent cell culture RS treated glass slide Proprietary wash |
For adherent cell culture chemically modified surface that’s
optimal for fastidious cells Mimics a poly-lysine coated. |
| Biological testing | BHK-21, Hep-2 cells | BHK-21, Hep-2 cells | Primary chick brain neurons, Hep-2 cells |
| Leak tested | Yes | Yes | Yes |
| Slide background fluorescence | Glass: Minimal fluorescence Permanox®: Minimal fluorescence Polystyrene: Autofluorescence at <485nm | Glass: • No endogenous fluorescence |
Glass: • No endogenous fluorescence |
| Coverslip compatibility | Yes, following gasket removal | Yes | Yes |
| Writing surface | “Sand-blasted” frosted end | Super Frost® white printed end | SuperFrost® blue printed end |
Lab-Tek® Chamber Slide Specifications: Well Visible Working
| Growth Surface | Well Size (cm2) | Visible Area (cm2) | Working Volume (ml) |
| 1 Chamber, Glass/Permanox | 9.4 | 9.4 | 2.5-4.5 |
| 2 Chamber, Glass/Permanox | 4.2 | 4.2 | 1.2-2.0 |
| 4 Chamber, Glass/Permanox | 1.8 | 1.8 | 0.5-0.9 |
| 8 Chamber, Glass/Permanox | 0.8 | 0.8 | 0.2-0.4 |
| 16 Chamber, Glass | 0.4 | 0.4 | 0.1-0.2 |
| Flaskette, Glass | 10.0 | 10.0 | 2.5-5.0 |
Lab-Tek® II Chamber Slide Specifications:
| Growth Surface | Well Size (cm2) | Visible Area (cm2) | Working Volume (ml) |
| 1 Chamber, Glass, CC2 | 10.0 | 8.6 | 4.0-5.0 |
| 2 Chamber, Glass, CC2 | 4.2 | 4.0 | 2.0-2.5 |
| 4 Chamber, Glass, CC2 | 2.4 | 1.7 | 1.0-1.3 |
| 8 Chamber, Glass, CC2 | 1.2 | 0.7 | 0.5-0.6 |
Lab-Tek® Chamber Slide™ System
- Developed for microscopy of in situ cell culture.
- Cells grow on a standard microscope slide.
- Media chamber can be removed when culturingis complete.
- Useful for viral and mycoplasma testing, chromosome studies toxicity tests and immunocytology.
- Permanox® plastic and glass do not interfere with fluorescent labels.
Permanox® is a special molding resin, which meets the growing requirements of most anchorage dependent cells; also excellent chemical resistance allows for cell fixation and the staining of organic solvents for EM and other techniques. Low oxygen permeation makes Permanox® ideal for anoxic cell growth.
Chamber slides combine a media-containing chambered super-structure with a standard soda lime glass slide or Permanox® plastic slide, sealing the two together with a biologically inert silicone gasket. After cells are grown, the chamber and gasket are easily removed. The slide may then be fixed, stained, cover slipped, and filed with the usual convenience. These slides, once fixed and stained, can be embedded using our Slide Duplicating Mold (Cat. 70170).
Lab-Tek® Chamber Slide™ System all come packed in a sterile sealed tray, 8 units per tray, 2 trays per pack and 12 trays per case.
Lab-Tek® Glass
Chamber Slide™
| 70381 | Glass Slide, 1 Chamber | 2 trays | $120.00 |  |
| 70384 | Glass Slide, 1 Chamber | 6 trays | $330.00 | |
| 70387 | Glass Slide, 1 Chamber | 12 trays | $588.00 | |
| 70391 | Glass Slide, 2 Chamber | 2 trays | $131.00 | |
| 70394 | Glass Slide, 2 Chamber | 6 trays | $344.00 | |
| 70397 | Glass Slide, 2 Chamber | 12 trays | $606.00 | |
| 70401 | Glass Slide, 4 Chamber | 2 trays | $132.00 | |
| 70404 | Glass Slide, 4 Chamber | 6 trays | $360.00 | |
| 70407 | Glass Slide, 4 Chamber | 12 trays | $643.00 | |
| 70411 | Glass Slide, 8 Chamber | 2 trays | $143.00 | |
| 70414 | Glass Slide, 8 Chamber | 6 trays | $400.00 | |
| 70415 | Glass Slide, 8 Chamber | 12 trays | $647.00 | |
Lab-Tek® Permanox® Chamber Slide™
| 70380 | Permanox® Slide, 1 Chamber | 2 trays | $136.00 | |
| 70383 | Permanox® Slide, 1 Chamber | 6 trays | $375.00 | |
| 70386 | Permanox® Slide, 1 Chamber | 12 trays | $695.00 | |
| 70390 | Permanox® Slide, 2 Chamber | 2 trays | $136.00 | |
| 70393 | Permanox® Slide, 2 Chamber | 6 trays | $376.00 | |
| 70396 | Permanox® Slide, 2 Chamber | 12 trays | $709.00 | |
| 70400 | Permanox® Slide, 4 Chamber | 2 trays | $145.00 | |
| 70403 | Permanox® Slide, 4 Chamber | 6 trays | $393.00 | |
| 70406 | Permanox® Slide, 4 Chamber | 12 trays | $733.00 | |
| 70410 | Permanox® Slide, 8 Chamber | 2 trays | $146.00 | |
| 70413 | Permanox® Slide, 8 Chamber | 6 trays | $390.00 | |
| 70416 | Permanox® Slide, 8 Chamber | 12 trays | $747.00 | |
Lab-Tek® Flaskettes and 16-Well
| 70417 | Flaskette, Glass | 2 trays | $131.00 | |
| 70418 | Flaskette, Glass | 6 trays | $370.00 | |
| 70419 | Flaskette, Glass | 12 trays | $692.00 | |
| 70420 | 16-Well, Glass | 2 trays | $130.00 | |
| 70421 | 16-Well, Glass | 6 trays | $360.00 | |
| 70422 | 16-Well, Glass | 12 trays | $709.00 | |
| 70423 | 16-Well, Coverglass, 22 x 74 mm | 2 oz/bx | $42.00 | |
Lab-Tek® II Chamber Slide System
- Sterile, Chamber Slide™ products have removable, polystyrene media chamber of 1, 2, 4, and 8-well configuration
- Low fluorescent glass microscope slide (25 x 75 x 1.2 mm) with rounded corners
- Biocompatible adhesive
- Polystyrene cover
- Inert hydrophobic well border printed on slide
- SuperFrost printed area
Lab-Tek® II Chamber Slide™ products offer optimal surface
for excellent attachment and growth of cells, easily distinguished
by the blue mark. This design allows for the removal of the media
chamber and adhesive from the slide, using a separation device.
The blue hydrophobic border is raised slightly and isolates the
individual wells, and prevents cross contamination. The slide
can be coversliped.
Lab-Tek® II CC2™ Chamber Slide™ products are the newest addition to our Lab-Tek® II Chamber Slide™ family. CC2 shares several similarities with the original Lab-Tek® II Chamber Slide, however, it’s pronounced difference is that the glass growth surface has been chemically modified. This modification provides an optimal surface for growing fastidious cells such as primary neurons or poorly adherent cell lines. CC2 also are non-auto fluorescent making them ideal for fluorescent applications such as FISH (Fluorescent in situ Hybridization). The growth surface remains stable without refrigeration.
Lab-Tek® II and Lab-Tek II CC2™ Chamber Slide™ Systems all come packed in a sterile sealed tray. 8 units per tray, 2 trays per pack and 12 trays per case.
Lab-Tek® II
Chamber Slide™
| 70379-12 | Lab-Tek® II Slide, 1 Chamber | 2 trays | $124.00 | |
| 70379-14 | Lab-Tek® II Slide, 1 Chamber | 6 trays | $325.00 | |
| 70379-16 | Lab-Tek® II Slide, 1 Chamber | 12 trays | $593.00 | |
| 70379-22 | Lab-Tek® II Slide, 2 Chamber | 2 trays | $136.00 | |
| 70379-24 | Lab-Tek® II Slide, 2 Chamber | 6 trays | $374.00 | |
| 70379-26 | Lab-Tek® II Slide, 2 Chamber | 12 trays | $626.00 | |
| 70379-42 | Lab-Tek® II Slide, 4 Chamber | 2 trays | $136.00 | |
| 70379-44 | Lab-Tek® II Slide, 4 Chamber | 6 trays | $374.00 | |
| 70379-46 | Lab-Tek® II Slide, 4 Chamber | 12 trays | $643.00 | |
| 70379-82 | Lab-Tek® II Slide, 8 Chamber | 2 trays | $143.00 | |
| 70379-84 | Lab-Tek® II Slide, 8 Chamber | 6 trays | $390.00 | |
| 70379-86 | Lab-Tek® II Slide, 8 Chamber | 12 trays | $663.00 | |
| 70360-84 | Lab-Tek® II CC2™ Slide, 8 Chamber | 6 trays | $520.00 | |
| 70360-86 | Lab-Tek® II CC2™ Slide, 8 Chamber | 12 trays | $961.00 | |
Lab-Tek® II
CC2™ Chamber Slide™
| 70360-12 | Lab-Tek® II CC2™ Slide, 1 Chamber | 2 trays | $166.00 | |
| 70360-14 | Lab-Tek® II CC2™ Slide, 1 Chamber | 6 trays | $460.00 | |
| 70360-16 | Lab-Tek® II CC2™ Slide, 1 Chamber | 12 trays | $873.00 | |
| 70360-22 | Lab-Tek® II CC2™ Slide, 2 Chamber | 2 trays | $176.00 | |
| 70360-24 | Lab-Tek® II CC2™ Slide, 2 Chamber | 6 trays | $495.00 | |
| 70360-26 | Lab-Tek® II CC2™ Slide, 2 Chamber | 12 trays | $901.00 | |
| 70360-42 | Lab-Tek® II CC2™ Slide, 4 Chamber | 2 trays | $188.00 | |
| 70360-44 | Lab-Tek® II CC2™ Slide, 4 Chamber | 6 trays | $515.00 | |
| 70360-46 | Lab-Tek® II CC2™ Slide, 4 Chamber | 12 trays | $931.00 | |
| 70360-82 | Lab-Tek® II CC2™ Slide, 8 Chamber | 2 trays | $189.00 | |
| 70360-84 | Lab-Tek® II CC2™ Slide, 8 Chamber | 6 trays | $520.00 | |
| 70360-86 | Lab-Tek® II CC2™ Slide, 8 Chamber | 12 trays | $961.00 | |
Lab-Tek¨ Chambered Coverglass
System
Lab-Tek® and Lab-Tek®II Chambered Coverglass products are intended for high magnification examination of living cells using an inverted microscope. The growth surface of the Lab-Tek Chambered Coverglass products are #1 Borosilicate Coverglass from American origin. The Lab-Tek¨ II Chambered Coverglass products are #1.5 Borosilicate Coverglass of German origin. The thin glass growth surface allows optimal optical clarity when viewing living cells with high resolution of an inverted microscope. The media chambers are available in 1, 2, 4, and 8 well formats and cannot be removed from the coverglass surface.
The Chambered Coverglass is provided sterile and ready to use.
| Product Features | Lab-Tek® | Lab-Tek® II |
| Configurations | 1, 2, 4, 8 well | 1, 2, 4, 8 well |
| Media chamber material | Proprietary cell culture grade polystyrene (PS) | Proprietary cell culture grade polystyrene (PS): non removable |
| Slide Materials | ||
| Cover | Clear polystyrene, no handle, facilitates microscopic viewing | Clear polystyrene, with handle for aseptic manipulations |
| Sealant | Non-toxic medical grade silicone |
Non-toxic medical grade silicone |
| Growth Surface | For adherent cell culture. Good growth with fastidious cells, e.g., neurons | For adherent cell culture. Good growth with fastidious cells, e.g., neurons |
| Biological testing | BHK-21, Hep-2 cells | BHK-21, Hep-2 cells |
| Leak tested | Yes | Yes |
| Slide background fluorescence | Glass: Minimal fluorescence | Glass: Minimal fluorescence |
| Coverslip compatibility | Yes, following gasket removal | Yes |
| Writing surface | none | none |
Lab-Tek® Chamber
Coverglass™
| 70378-11 | L.T. Coverglass, 1 Chamber | 2 trays | $143.00 | |
| 70378-12 | L.T. Coverglass, 1 Chamber | 12 trays | $643.00 | |
| 70378-21 | L.T. Coverglass, 2 Chamber | 2 trays | $145.00 | |
| 70378-22 | L.T. Coverglass, 2 Chamber | 12 trays | $660.00 | |
| 70378-41 | L.T. Coverglass, 4 Chamber | 2 trays | $154.00 | |
| 70378-42 | L.T. Coverglass, 4 Chamber | 12 trays | $701.00 | |
| 70378-81 | L.T. Coverglass, 8 Chamber | 2 trays | $154.00 | |
| 70378-82 | L.T. Coverglass, 8 Chamber | 12 trays | $692.00 | |
Lab-Tek® II Chamber Coverglass™
For Inverted Microscope
| 70377-11 | L.T. II Coverglass, 1 Chamber | 2 trays | $156.00 | |
| 70377-12 | L.T. II Coverglass, 1 Chamber | 12 trays | $769.00 | |
| 70377-21 | L.T. II Coverglass, 2 Chamber | 2 trays | $176.00 | |
| 70377-22 | L.T. II Coverglass, 2 Chamber | 12 trays | $809.00 | |
| 70377-41 | L.T. II Coverglass, 4 Chamber | 2 trays | $187.00 | |
| 70377-42 | L.T. II Coverglass, 4 Chamber | 12 trays | $840.00 | |
| 70377-81 | L.T. II Coverglass, 8 Chamber | 2 trays | $199.00 | |
| 70377-82 | L.T. II Coverglass, 8 Chamber | 12 trays | $877.00 | |
