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Electron Microscopy Sciences

Specimen Preparation and Embedding Supplies

arrow14Lab-Tek® Chamber Slide Products

Lab-Tek® Chamber Slidearrow12Lab-Tek® Chamber Slide™ System: Lab-Tek® Chamber Slide™, Lab-Tek® II Chamber Slide™ and Lab-Tek® II CC2™ Chamber Slide™ Products

The growth of various cell types on plastic, soda lime glass and borosilicate coverglass were examined. The surfaces were either unmodified, coated with polylysine, or stably surface modified with non-biological reagents as described by Kleinfield, et al. (1988). All substrates were assembled into Lab-Tek® and Lab-Tek®II Chamber Slide™ products.

Product Features Lab-Tek® Lab-Tek® II Lab-Tek® II CC2
Configurations 1, 2, 4, 8, 16 well, flask style 1, 2, 4, 8 well 1, 2, 4, 8 well
Media chamber material Proprietary cell culture grade polystyrene (PS) Proprietary cell culture grade polystyrene (PS) Proprietary cell culture grade polystyrene (PS)
Slide Materials Soda lime glass Permanox® plastic Polystyrene plastic
Soda lime glass Soda lime glass
Cover Clear polystyrene, no handle, facilitates microscopic viewing Clear polystyrene, with handle for aseptic manipulations Clear polystyrene, with handle for aseptic manipulations
Sealant Non-toxic medical grade silicone
• Remains on slide after detachment
Biocompatible acrylic adhesive
• Remains with media chamber after detachment
Biocompatible acrylic adhesive
• Remains with media chamber after detachment
Reagent ‘isolator’ Created by removable
silicone gasket
Created by hydrophobic border Created by hydrophobic border

Chamber separation
No separation tool required Requires separation tools Requires separation tools
Growth Surface For adherent cell culture Glass slides
• 6-stage high purity water wash
Permanox® plastic
• Cell culture treated
Polystyrene
• Cell culture treated
For adherent cell culture RS treated glass slide
Proprietary wash
For adherent cell culture chemically modified surface that’s optimal for fastidious cells
Mimics a poly-lysine coated.
Biological testing BHK-21, Hep-2 cells BHK-21, Hep-2 cells Primary chick brain neurons, Hep-2 cells
Leak tested Yes Yes Yes
Slide background fluorescence Glass: Minimal fluorescence Permanox®: Minimal fluorescence Polystyrene: Autofluorescence at <485nm Glass:
• No endogenous fluorescence
Glass:
• No endogenous fluorescence
Coverslip compatibility Yes, following gasket removal Yes Yes
Writing surface “Sand-blasted” frosted end Super Frost® white printed end SuperFrost® blue printed end

Lab-Tek® Chamber Slide Specifications: Well Visible Working

Growth Surface Well Size (cm2) Visible Area (cm2) Working Volume (ml)
1 Chamber, Glass/Permanox 9.4 9.4 2.5-4.5
2 Chamber, Glass/Permanox 4.2 4.2 1.2-2.0
4 Chamber, Glass/Permanox 1.8 1.8 0.5-0.9
8 Chamber, Glass/Permanox 0.8 0.8 0.2-0.4
16 Chamber, Glass 0.4 0.4 0.1-0.2
Flaskette, Glass 10.0 10.0 2.5-5.0

Lab-Tek® II Chamber Slide Specifications:

Growth Surface Well Size (cm2) Visible Area (cm2) Working Volume (ml)
1 Chamber, Glass, CC2 10.0 8.6 4.0-5.0
2 Chamber, Glass, CC2 4.2 4.0 2.0-2.5
4 Chamber, Glass, CC2 2.4 1.7 1.0-1.3
8 Chamber, Glass, CC2 1.2 0.7 0.5-0.6

Lab-Tek® Chamber Slide™ System

  • Developed for microscopy of in situ cell culture.
  • Cells grow on a standard microscope slide.
  • Media chamber can be removed when culturingis complete.
  • Useful for viral and mycoplasma testing, chromosome studies toxicity tests and immunocytology.
  • Permanox® plastic and glass do not interfere with fluorescent labels.

Permanox® is a special molding resin, which meets the growing requirements of most anchorage dependent cells; also excellent chemical resistance allows for cell fixation and the staining of organic solvents for EM and other techniques. Low oxygen permeation makes Permanox® ideal for anoxic cell growth.

Chamber slides combine a media-containing chambered super-structure with a standard soda lime glass slide or Permanox® plastic slide, sealing the two together with a biologically inert silicone gasket. After cells are grown, the chamber and gasket are easily removed. The slide may then be fixed, stained, cover slipped, and filed with the usual convenience. These slides, once fixed and stained, can be embedded using our Slide Duplicating Mold (Cat. 70170).

Lab-Tek® Chamber Slide™ System all come packed in a sterile sealed tray, 8 units per tray, 2 trays per pack and 12 trays per case.

arrow12Lab-Tek® Glass Chamber Slide™
70381 Glass Slide, 1 Chamber 2 trays 147.00 Add to Cart
70384 Glass Slide, 1 Chamber 6 trays 415.00 Add to Cart
70387 Glass Slide, 1 Chamber 12 trays 707.00 Add to Cart
70391 Glass Slide, 2 Chamber 2 trays 159.00 Add to Cart
70394 Glass Slide, 2 Chamber 6 trays 417.00 Add to Cart
70397 Glass Slide, 2 Chamber 12 trays 727.00 Add to Cart
70401 Glass Slide, 4 Chamber 2 trays 158.00 Add to Cart
70404 Glass Slide, 4 Chamber 6 trays 430.00 Add to Cart
70407 Glass Slide, 4 Chamber 12 trays 755.00 Add to Cart
70411 Glass Slide, 8 Chamber 2 trays 168.00 Add to Cart
70414 Glass Slide, 8 Chamber 6 trays 500.00 Add to Cart
70415 Glass Slide, 8 Chamber 12 trays 897.00 Add to Cart
arrow12Lab-Tek® Permanox® Chamber Slide™
70380 Permanox® Slide, 1 Chamber 2 trays 165.00 Add to Cart
70383 Permanox® Slide, 1 Chamber 6 trays 455.00 Add to Cart
70386 Permanox® Slide, 1 Chamber 12 trays 836.00 Add to Cart
70390 Permanox® Slide, 2 Chamber 2 trays 166.00 Add to Cart
70393 Permanox® Slide, 2 Chamber 6 trays 478.00 Add to Cart
70396 Permanox® Slide, 2 Chamber 12 trays 850.00 Add to Cart
70400 Permanox® Slide, 4 Chamber 2 trays 178.00 Add to Cart
70403 Permanox® Slide, 4 Chamber 6 trays 490.00 Add to Cart
70406 Permanox® Slide, 4 Chamber 12 trays 879.00 Add to Cart
70410 Permanox® Slide, 8 Chamber 2 trays 178.00 Add to Cart
70413 Permanox® Slide, 8 Chamber 6 trays 488.00 Add to Cart
70416 Permanox® Slide, 8 Chamber 12 trays 897.00 Add to Cart
arrow12Lab-Tek® Flaskettes and 16-Well
70417 Flaskette, Glass 2 trays 162.00 Add to Cart
70418 Flaskette, Glass 6 trays 463.00 Add to Cart
70419 Flaskette, Glass 12 trays 832.00 Add to Cart
70420 16-Well, Glass 2 trays 162.00 Add to Cart
70421 16-Well, Glass 6 trays 430.00 Add to Cart
70422 16-Well, Glass 12 trays 853.00 Add to Cart
70423 16-Well, Coverglass, 22 x 74 mm 2 oz/bx 46.00 Add to Cart

Lab-Tek® II Chamber Slide Systemarrow12Lab-Tek® II Chamber Slide System

  • Sterile, Chamber Slide™ products have removable, polystyrene media chamber of 1, 2, 4, and 8-well configuration
  • Low fluorescent glass microscope slide (25 x 75 x 1.2 mm) with rounded corners
  • Biocompatible adhesive
  • Polystyrene cover
  • Inert hydrophobic well border printed on slide
  • SuperFrost printed area

Lab-Tek® II Chamber Slide SystemLab-Tek® II Chamber Slide™ products offer optimal surface for excellent attachment and growth of cells, easily distinguished by the blue mark. This design allows for the removal of the media chamber and adhesive from the slide, using a separation device. The blue hydrophobic border is raised slightly and isolates the individual wells, and prevents cross contamination. The slide can be coversliped.

Lab-Tek® II CC2™ Chamber Slide™ products are the newest addition to our Lab-Tek® II Chamber Slide™ family. CC2 shares several similarities with the original Lab-Tek® II Chamber Slide, however, it’s pronounced difference is that the glass growth surface has been chemically modified. This modification provides an optimal surface for growing fastidious cells such as primary neurons or poorly adherent cell lines. CC2 also are non-auto fluorescent making them ideal for fluorescent applications such as FISH (Fluorescent in situ Hybridization). The growth surface remains stable without refrigeration.

Lab-Tek® II and Lab-Tek II CC2™ Chamber Slide™ Systems all come packed in a sterile sealed tray. 8 units per tray, 2 trays per pack and 12 trays per case.

arrow12Lab-Tek® II Chamber Slide™
70379-12 Lab-Tek® II Slide, 1 Chamber 2 trays 147.00 Add to Cart
70379-14 Lab-Tek® II Slide, 1 Chamber 6 trays 388.00 Add to Cart
70379-16 Lab-Tek® II Slide, 1 Chamber 12 trays 712.00 Add to Cart
70379-22 Lab-Tek® II Slide, 2 Chamber 2 trays 160.00 Add to Cart
70379-24 Lab-Tek® II Slide, 2 Chamber 6 trays 435.00 Add to Cart
70379-26 Lab-Tek® II Slide, 2 Chamber 12 trays 751.00 Add to Cart
70379-42 Lab-Tek® II Slide, 4 Chamber 2 trays 160.00 Add to Cart
70379-44 Lab-Tek® II Slide, 4 Chamber 6 trays 430.00 Add to Cart
70379-46 Lab-Tek® II Slide, 4 Chamber 12 trays 756.00 Add to Cart
70379-82 Lab-Tek® II Slide, 8 Chamber 2 trays 170.00 Add to Cart
70379-84 Lab-Tek® II Slide, 8 Chamber 6 trays 458.00 Add to Cart
70379-86 Lab-Tek® II Slide, 8 Chamber 12 trays 779.00 Add to Cart
70360-84 Lab-Tek® II CC2™ Slide, 8 Chamber 6 trays 582.00 Add to Cart
70360-86 Lab-Tek® II CC2™ Slide, 8 Chamber 12 trays 1,131.00 Add to Cart
Lab-Tek® II CC2™ Chamber Slide arrow12Lab-Tek® II ™Chamber Slide™
70360-12 Lab-Tek® II CC2™ Slide, 1 Chamber 2 trays 202.00 Add to Cart
70360-14 Lab-Tek® II CC2™ Slide, 1 Chamber 6 trays 530.00 Add to Cart
70360-16 Lab-Tek® II CC2™ Slide, 1 Chamber 12 trays 1,046.00 Add to Cart
70360-22 Lab-Tek® II CC2™ Slide, 2 Chamber 2 trays 204.00 Add to Cart
70360-24 Lab-Tek® II CC2™ Slide, 2 Chamber 6 trays 660.00 Add to Cart
70360-26 Lab-Tek® II CC2™ Slide, 2 Chamber 12 trays 1,082.00 Add to Cart
70360-42 Lab-Tek® II CC2™ Slide, 4 Chamber 2 trays 211.00 Add to Cart
70360-44 Lab-Tek® II CC2™ Slide, 4 Chamber 6 trays 582.00 Add to Cart
70360-46 Lab-Tek® II CC2™ Slide, 4 Chamber 12 trays 1,095.00 Add to Cart
70360-82 Lab-Tek® II CC2™ Slide, 8 Chamber 2 trays 214.00 Add to Cart
70360-84 Lab-Tek® II CC2™ Slide, 8 Chamber 6 trays 582.00 Add to Cart
70360-86 Lab-Tek® II CC2™ Slide, 8 Chamber 12 trays 1,131.00 Add to Cart

Lab-Tek¨ Chambered Coverglass Systemarrow12Lab-Tek¨ Chambered Coverglass

Lab-Tek® and Lab-Tek®II Chambered Coverglass products are intended for high magnification examination of living cells using an inverted microscope. The growth surface of the Lab-Tek Chambered Coverglass products are #1 Borosilicate Coverglass from American origin. The Lab-Tek¨ II Chambered Coverglass products are #1.5 Borosilicate Coverglass of German origin. The thin glass growth surface allows optimal optical clarity when viewing living cells with high resolution of an inverted microscope. The media chambers are available in 1, 2, 4, and 8 well formats and cannot be removed from the coverglass surface.

The Chambered Coverglass is provided sterile and ready to use.

Product Features Lab-Tek® Lab-Tek® II
Configurations 1, 2, 4, 8 well 1, 2, 4, 8 well
Media chamber material Proprietary cell culture grade polystyrene (PS) Proprietary cell culture grade polystyrene (PS): non removable
Slide Materials
 
Cover Clear polystyrene, no handle, facilitates microscopic viewing Clear polystyrene, with handle for aseptic manipulations
Sealant Non-toxic medical grade silicone Non-toxic medical grade silicone
Growth Surface For adherent cell culture. Good growth with fastidious cells, e.g., neurons For adherent cell culture. Good growth with fastidious cells, e.g., neurons
Biological testing BHK-21, Hep-2 cells BHK-21, Hep-2 cells
Leak tested Yes Yes
Slide background fluorescence Glass: Minimal fluorescence Glass: Minimal fluorescence
Coverslip compatibility Yes, following gasket removal Yes
Writing surface none none
arrow12Lab-Tek® Chamber Coverglass™
70378-11 L.T. Coverglass, 1 Chamber 2 trays 168.00 Add to Cart
70378-12 L.T. Coverglass, 1 Chamber 12 trays 780.00 Add to Cart
70378-21 L.T. Coverglass, 2 Chamber 2 trays 170.00 Add to Cart
70378-22 L.T. Coverglass, 2 Chamber 12 trays 802.00 Add to Cart
70378-41 L.T. Coverglass, 4 Chamber 2 trays 180.00 Add to Cart
70378-42 L.T. Coverglass, 4 Chamber 12 trays 851.00 Add to Cart
70378-81 L.T. Coverglass, 8 Chamber 2 trays 180.00 Add to Cart
70378-82 L.T. Coverglass, 8 Chamber 12 trays 869.00 Add to Cart
Lab-Tek® II Chamber Coverglassarrow12Lab-Tek® II Chamber Coverglass™

For Inverted Microscope

70377-11 L.T. II Coverglass, 1 Chamber 2 trays 187.00 Add to Cart
70377-12 L.T. II Coverglass, 1 Chamber 12 trays 934.00 Add to Cart
70377-21 L.T. II Coverglass, 2 Chamber 2 trays 208.00 Add to Cart
70377-22 L.T. II Coverglass, 2 Chamber 12 trays 983.00 Add to Cart
70377-41 L.T. II Coverglass, 4 Chamber 2 trays 222.00 Add to Cart
70377-42 L.T. II Coverglass, 4 Chamber 12 trays 1,021.00 Add to Cart
70377-81 L.T. II Coverglass, 8 Chamber 2 trays 236.00 Add to Cart
70377-82 L.T. II Coverglass, 8 Chamber 12 trays 1,067.00 Add to Cart

Wells, Optical Bottom Plates, Cell Culture Inserts arrow12arrow12