Cell Culture, Petri, and Permanox Dishes and Seals

Parafilm M and Dispenser

Laboratory film used to prevent evaporation, conta-mination and hydration. Seals labware quickly and efficiently. Used in EM for drop staining and to pick up thin films when coating grids.

70990	4"x250', 1" core	each	31.00
70991	2"x250', 1" core	each	20.00
70992	Cutter dispenser	each	75.00

DuraSeal™

A polyethylene based durable stretch film which withstands heat and solvents. It has many substantial advantages over commonly used laboratory stretch films.

Advantages:

• Withstands high heat (110°C) and is microwave compatible.
• Chemically resistant- Resists essentially all solvents and caustic agents.
• High tear strength- it can stretch 500-800% before breakage or tearing.
• Cling - the film can cling to itself following stretching.
• Tight sealing -it forms an air tight barrier.

Permeability Information:

Oxygen: 125cc/100in²/24 hrs at 23°C
Carbon Dioxide: 1300cc/100in²/24 hrs at 23°C
Water Vapor: 1.0g/100in²/24 hrs at 38°C and 90% R.H.

70994-02	DuraSeal 2"x500ft	each	34.00
70994-04	DuraSeal 4"x250ft	each	34.00
70994-05	DuraSeal 4"x500ft	each	50.00
70994-20	DuraSeal Dispenser	each	8.50

PetriStickers™

Stickers for Labeling Petri Dishes

Label Petri dishes quickly, neatly, and reliably.

• Temperature Resistant – Labels remain firmly intact in temperatures from 4ºC to 42°C
• Convenient – Instantly form an imprint Petri dish for quick reference, then remove if you wish
• Variety Pattern – Offering many choices to suit your work: Counting or Streaking

PetriStickers™ are 3" or 76 mm diameter.

Ordering

70630-32	32 - Square Grid	36/pk.	18.00
70630-50	50 - Squre Grid	36/pk.	18.00
70630-70	70 - Square Grids	36/pk.	18.00
70630-100	100 - Square Grids	36/pk.	18.00
70631-06	6 - Sector Pie	36/pk.	18.00
70631-08	8 - Sector Pie	36/pk.	18.00
70631-12	12 - Sector Pie	36/pk.	18.00
70631-16	16 - Sector Pie	36/pk.	18.00

PetriSEAL™ and ContainerSEAL™

Ideal sealing Tapes for Petri Dishes, Microplates, Lids, Caps and other Lab Items.

• Conformity – Stretches, conforms and seals the irregular perimeter of microplates or Petri dishes
• Waterproof & Chemical Resistant – Tapes and adhesive resist most solvents and caustic agents
• Temperature Resistant – Seal remains flexible in moderate freezer temperatures and withstands intermittent exposure to 100°C

Ordering

70500-B	PetriSEAL, Blue	0.50 x 108	Roll	34.00
70500-C	PetriSEAL, Clear	0.50 x 108	Roll	34.00
70500-R	PetriSEAL, Red	0.50 x 108	Roll	34.00
70500-Y	PetriSEAL, Yellow	0.50 x 108	Roll	34.00
70500-W	PetriSEAL, White	0.50 x 108	Roll	35.00
70501-R	ContainerSEAL, Red	0.75 x 108	Roll	40.00
70501-W	ContainerSEAL, White	0.75 x 108	Roll	40.00
70501-Y	ContainerSEAL, Yellow	0.75 x 108	Roll	40.00

Dissecting Dish

A silicone pad placed inside a 5½" dia. round petri dish. The mat can be removed, washed, and returned to the dish for reuse. The pins come in 10 color coded pins. An optional Pyrex Glass Petri dish is available for use when sterilization is needed.

70540	Dissecting Dish with Plastic Dish	each	16.00
70540-50	Dissecting Dish with Pyrex® Dish	each	126.00

Embryo Dishes

These dishes are molded from clear glass and come with a glass lid for the viewing or staining of free-floating specimens.

70543-30	Embryo Dish, 30 mm Dia x 12mm Deep	each	$6.00
70543-45	Embryo Dish, 45 mm Dia x 12mm Deep	each	$12.00

Glass Culture Dishes - Petri Dishes

KIMAX® Brand - Designed from Federal Specification

KIMAX® dishes have reinforced beaded edges to resist mecanical breakage and to help in centering the bottom inside the cover. Dishes remain clear after repeated use in wet or dry sterilization cycles. Covers have a fused-on bright red ceramic enamel marking spot and inscription, including "bottom". The combination of different colors and wording permits quick and easy identification of the parts when sorting, sealing and assembling pairs. The bottom also has a vertical arrow on the side to precisely locate serial dilution starting points. Shelf-packs contain twelve covers and twelve buttons, which are packed in seperate boxes.

Catalog #	Approx. Dia.	Approx. Height	Qty.	Price
70648-10	60mm	15mm	12/pk	78.00
70648-14	100mm	15mm	12/pk	78.00
70648-18*	150mm	20mm	12/pk	325.00

* This item comes without lid.

Petri Dish; Analyslide

The analyslide is like a petri dish on a slide. Use it as an examination and storage container for 47mm filters. Its rectangular base fits any microscope stage; an inner cover ring offers airtight security for the samples and it does not interfere with viewing...It is also ideal for drying coated grids and absorbing water from grids that are supporting sections.

70650

Analyslide

100/pk

65.00

Petri Dishes; Open with One Hand

A 50x9mm petri dish design that is easily opened with one hand, yet closes to a tight seal by its unique double rim. Specimen samples are preserved until you are ready to analyze them. Humidity remains locked in. When loaded with a 47mm absorbent pad, it is ideal for drying coated grids, removing water from grids that are supporting sections.

Available with or without absorbent pads. The dishes are sterilized by Gamma Irradiation

70660	Without Absorbent pads	100/pk	48.00
70662	With Absorbent pads	100/pk	51.00
70664	Absorbent pad kit: (1000 pads, 47mm, and dispenser)	Kit	140.00

Petri Dishes; Disposable

Our petri dishes are available in a variety of sizes and shapes for use in routine procedures and automatic equipmentF. Manufactured from Polystyrene, with excellent optical clarity. The dishes are sterile and each lot is tested to assure uniform quality and sterility. Sterlstar Petri Dishes are designed for the Microbiologist who prefers a heavier dish: The Sterlstar dish offers easy handling, a secure grip, and additional strength.

70680	100x15mm Standard Round	100/pk	28.00
70682	100x15mm Bi-Petri	100/pk	34.00
70684	100x15mm Tri-Petri	100/pk	36.00
70686	100x15mm Quad-Petri	100/pk	34.00
70690	110x15mm Square	100/pk	62.00

Specialized Dishes

35x10mm Untreated Polystyrene Dish

35x10mm dish for the culture of cells in suspension; radiation sterilized.

60x15mm Permanox Dish

Permanox is a strong, biologically inert material, resistant to nonchlorinated hydrocarbons, acetone, and many other EM reagents. Our EMbed-812 and other embedding resins can be used to embed cells in these dishes with excellent results.

Permanox has not been shown to be autofluorescent, and has a low oxygen solubility which makes it suitable for experiments performed under anoxic conditions.

The dishes have high optical quality that provide excellent and consistent viewing where microscopic examination is required.

70340	35x10mm Suspension Dish	100/pk	60.00
70342	35x10mm Suspension Dish	500/pk	298.00
70350	60x15mm Permanox Dish	40/pk	64.00
70353	60x15mm Permanox Dish	100/pk	138.00
70356	60x15mm Permanox Dish	500/pk	623.00

Cover Glass Bottom Sterile Culture Dish

EMS introduces the High Quality Thin Glass Bottom Sterile Culture Dishes, meeting your research requirements.

Ideal For:

• Fluorescence microscopy
• High resolution image analysis
• Electrophysiology
• Confocal (laser) microscopy
• Cell culture

A new series of sterile, optical quality, glass bottom dishes which provide exceptional imaging quality for many applications requiring the use of inverted microscopes. Conventional plastic or glass petri dishes and chambers limit the use of the inverted microscope for many applications because the thick plasti or glass bottom requires a long working distance objetive available only in lower magnifications. Moreover, plastic dishes cannot be used for DIC or any polarization microscopes due to their inherent birefriengence.

EMS New Glass Bottom Dishes eliminates these problems, making them ideal for applications such as: patch clamp recordings where flourescent-tagged (GFP) receptors can be identified for selective study; and many other fluorescent microscopy applications, which require low background scattering of light and reduce intrinsic "auto" fluorescence that occurs when using standard plastic dishes.

Furthermore, the glass bottom is designed flush with the whole base of the dish, therefore allowing direct contact between the entire glass surface and the warming stage. This eliminates any air gaps that may otherwise exist between the glass dish and the warming stage and hence optimize heat transfer, allowing homogenerous heating and cooling of the glass bottom.

Features:

• Non-cytotoxic, non-pyrogenic and sterile until opened.
• Dish diameters: 35 and 50mm, with internal gass well diameter of 12, 22, 30, and 40mm.
• Available sterile either individually packed in a medical-style blister or in sleeves of 20 dishes.
• Each dish has a flat (0.17mm thick) optical quality glass bottom.
• Production is carried on in a Class 10,000 clean room, certified under EN46001, EN46002, ISO9001 and ISO9002.
• All dishes are gamma irradiated (EN552) and sterile.

Optical Properties:

• Wide optical range of transmission- UV, IV, and IR (300nm to 2500nm).
• Flat (0.17mm thick) optical quality glass bottom provides the following advantages:
• Use of a much shorter working distance immersion objective.
• Larger numerical aperture (NA) objectives.
• Higher magnification (up to 100x)

Microscopy, Imaging, and Spectroscopy:

• Confocal (laser) microscopy.
• Two-/multi Photon Confocal Microscopy.
• DIC and Polarization Microscopy.
• High-resolution Imaging System.
• Infrared imaging (e.g., brain slide recordings).

Some Applications:

• Cell Biology: STEM Cell Research, Cell growth cycle monitoring, cell culture.
• Protein Chemistry: Green fluorescent protein identification of Histotags, etc.
• Molecular Biology: Phenotyping and other complex Genetic Research.

Ordering:

Individual Blister Pack
Dish Size (Dia. x H)	Glass Well Dia.	20/pk	Price		120/cs	Price
35 x 10mm	12 mm	70670-02	106.00		70670-12	620.00
	22 mm	70671-02	117.00		70671-12	620.00
50 x 7mm	30 mm	70673-02	106.00		70673-12	620.00
	40 mm	70674-02	117.00		70674-12	620.00
Bulk Pack in Sleeve
Dish Size (Dia. x H)	Glass Well Dia.	20/pk	Price		120/cs	Price
35 x 10mm	12 mm	70670-52	106.00		70670-62	785.00
	22 mm	70671-52	106.00		70671-62	785.00
50 x 7mm	30 mm	70673-52	106.00		70673-62	785.00
	40 mm	70674-52	106.00		70674-62	785.00

Cellattice™ - Micro-Ruled Plastic Cell Culture Surfaces

Purposes:

Measure the Same Cell: Cell cycle, axon growth and differentiation
Direct Cell Count: Proliferation and cytotoxicity without removing attached cells

Introduction

Cellattice™ micro-ruled Plastic Cell Culture Surfaces consists of a cell culture surface with microscopic identification and measurement markers. It is manufactured with high optical quality plastic suitable for phase contrast and other standard cell-based assay measurement techniques. Cell cultures on the Cellatice™ are identifiable within 25 micrometers.

The built-in markers on the Cellattice allow direct measurement of cell growth and movement without image acquisition. Same cell or cell clusters can be measured throughout the time course of the experiment, even after each incubation period.

Cellatice™ can be used to monitor morphology, cell movement and differentiation at the individual cell level. Cell proliferation is measured directly with multiple reading.

Cellattice™ Coverslips and Slides

Benefits:

• Measure axon growth directly without image acquisition and image analysis
• Count Cells after attachment
• Measure cell proliferation directly with multiple readings without detaching
• Monitor same cell or cell cluster for morphology, cell movement and differentiation
  

Catalog #	Size	Thickness	Grid Area	Pack	Price
63570-15	15 x 15 mm	0.12 – 0.16 mm	10 x 10 mm	50	74.00
63571-25	25 mm Dia	0.12 – 0.16 mm	10 x 10 mm	50	106.00
63572-75	25 x 75 mm	0.76 x 0.78 mm	10 x 10 mm	50	160.00

Tissue Processing Well; Netwell™

A modular and disposable system which can be used for the following procedures:

• The handling and processing of tissue slices.
• In immunocytochemical staining.
• In 3-dimensional tissue culture.
• In explant tissue culture.

Home made devices are no longer necessary. These tissue processing wells are ready to fill all of your needs with the added convenience of being able to process up to 12 tissue specimens at one time (with one unit) .

Netwell™ Inserts

The Netwell™ is a polystyrene insert fitted with a polyester mesh bottom for use in 6 and 12-well plates. Available in three mesh sizes and two diameters; the Netwells provide ultimate flexibility in retaining fragile tissues during processing and staining.

Netwell™ Carrier Kit

A set of carrier plates and handles for moving the Netwells in and out of the 6 or 12-well plates. The handle can be attached or detached to the plate at any time.

Netwell™ Reagent Tray

Thermo-formed polystyrene. Holds 150ml of reagent solution. Netwells are trans-ferred to the reagent tray by the Carrier kit, where fixing and staining is performed. Reagent trays are available in white for colorimetric reaction contrast, and in black for better visibility of tissue sections.

Applications

• The polyester mesh acts as a tissue carrier and strainer for immunocytochemical staining and biological and histological fixation. It avoids the risk of losing valuable samples associated with paint-brush transfer.
• For 3-D tissue culture, the sterile Netwell provides efficient media permeation and support to cultures grown in gels.
• As well, Sterile Netwells allow for easy processing of tissue explants and microcarrier-grown cells.

Ordering Information:

Catalog #	Dia.	Mesh Size	Sterile	Units/cs	Price
64710-00	15 mm	74 µm	Yes	48	265.00
64711-00	15 mm	500 µm	Yes	48	265.00
64712-00	24 mm	74 µm	Yes	48	320.00
64713-00	24 mm	500 µm	Yes	48	320.00

Netwell™ Accessories

Catalog #	Description	Units/cs	Price
64730-00	Netwell Reagent Tray, Black	200	190.00
64730-10	Netwell Reagent Tray, Black	10	14.00
64732-00	Netwell Reagent Tray, White	200	190.00
64732-10	Netwell Reagent Tray, White	10	14.00
64735-00	Netwell Carrier Kit, 15 mm	8	160.00
64735-01	Netwell Carrier Kit, 15 mm	1	22.00
64736-00	Netwell Carrier Kit, 24 mm	8	160.00
64736-01	Netwell Carrier Kit, 24 mm	1	22.00

For your convenience, the Netwell™ Sets are available ready to use.

The set consists of:

• 6 Netwells of 24mm diameter or 12 Netwells of 15mm diameter.
• Two Reagent Trays, one black and one white.
• One Carrier Kit: one carrier plate and one handle.

Catalog #	Netwell™ Dia.	Netwell™ Mesh	Pack	Price
64740-00	15 mm, Sterile	74µm	set	80.00
64742-00	15 mm, Sterile	500µm	set	80.00
64743-00	24 mm, Sterile	74µm	set	80.00
64745-00	24 mm, Sterile	500µm	set	80.00

Costar® Brand Cell Culture Clusters

Tissue culture plates are designed and treated for a wide range of applications.

Plates are made from optically clear, medical grade polystyrene with flat bottoms of uniform thickness, allowing for distortion-free observation and microscopic examination. Alphanumeric coordinates are placed on the same focal plane as cell growth—for easy referencing of well position.

These plates are also for use with our NetWells and our MeshWell Plates (see Tissue Processing Well; Net- Wells, and MeshWell Plates in our product catalog page 180).

Clusters are commonly used in the following applications:

• Maintenance of stock cultures
• Cell cloning
• Hybridoma fusions
• Scale up culture
• Clone expansion
• 3H-Thymidine
• 51Cr Release assay
• MTT assays
• In vitro toxicology
• Drug binding studies
• Virus isolation
• Viral plaque assay
• Transformation
• Carcinogenicity testing
• Mutagenicity testing

Ordering:

Well Well Well Growth Total Well Working Packaging Corning Catalog Quantity
Format Dia, mm Area, ml Volume Volume Costar No Number

Well Format	Well Dia mm	Growth Area, ml	Total Well Vol	Working Volume
6	35	9.5	17 ml	5 ml
12	22.6	4	7 ml	3 ml
24	16	2	3.4 m	2 ml

64705-05	Costar Cell Culture Clusters	3506	5/bag	100/pk	238.00
64705-01	Costar Cell Culture Clusters	3516	1/bag	50/pk	136.00
64706-05	Costar Cell Culture Clusters	3512	5/bag	100/pk	270.00
64706-01	Costar Cell Culture Clusters	3513	1/bag	50/pk	160.00
64707-05	Costar Cell Culture Clusters	3527	5/bag	100/pk	280.00
64707-01	Costar Cell Culture Clusters	3526	1/tray	50/pk	150.00

MeshWell Plates; Section Processing and Immunocytochemical Trays

These plates are made from polypropylene and the bottom of each of the wells is an ETFE monofilament screening fabric with a 500µm (0.0197”) opening.

These MeshWell Plates are available with 6, 12, and 24 well configurations, and they fit into the 6, 12 and 24 Costar/Corning Cell Culture Clusters listed