Microarray Hybridization Systems
A.
LifterSlip™ Premium Printed Coverglass
LifterSlip™ Premium
Printed Coverglass
A. LifterSlip™ Premium Printed Coverglass
LifterSlip™ offers a simple solution to the problem of hybridization non-uniformity. An innovative raised edge design provides separation and allows for even dispersal of hybridization solution between the DNA chip and coverslip. LifterSlips™ allow for increased data quality by eliminating gradients caused by floating standard coverslips on hybridization solutions. The printed bars allow for easy injection of hybridization solution under the pre-positioned coverslips. Capillary attraction ensures that LifterSlip™ stays in place.
Depending on size and design configuration LifterSlip™ can hold a range of volumes.
LifterSlip™ is #2 thickness (0.20-0.22 mm) and the bar height is 0.04-0.06 mm.
| Cat. # | Bar Width (mm) |
Approx. Volume |
Size (mm) | Approx. Pieces per oz. |
Price per oz. |
|
| 72184-18 | .75 | 7.55µl | 18 x 18 | 160 | $72.00 |  |
| 72184-22 | .75 | 11.46µl | 22 x 22 | 107 | $72.00 | |
| 72184-25 | .75 | 13.02µl | 22 x 25 | 94 | $72.00 | |
| 72184-30 | .75 | 15.63µl | 22 x 30 | 78 | $72.00 | |
| 72184-40 | .75 | 20.84µl | 22 x 40 | 59 | $72.00 | |
| 72184-44 | .75 | 22.90µl | 22 x 44 | 53 | $72.00 | |
| 72184-50 | .75 | 26.05µl | 22 x 50 | 47 | $72.00 | |
| 72184-60 | .75 | 31.25µl | 22 x 60 | 39 | $72.00 | |
| 72185-24 | .75 | 13.72µl | 24 x 24 | 90 | $72.00 | |
| 72185-60 | .75 | 34.30µl | 24 x 60 | 36 | $72.00 | |
| 72185-62 | .75 | 35.07µl | 24 x 60 | 37 | $72.00 | |
| 72186-25 | .75 | 14.93µl | 25 x 25 | 83 | $72.00 | |
| 72186-35 | .75 | 20.90µl | 25 x 35 | 59 | $72.00 | |
| 72186-36 | .75 | 21.50µl | 25 x 36 | 58 | $72.00 | |
| 72186-40 | .75 | 23.89µl | 25 x 40 | 52 | $72.00 | |
| 72186-42 | .75 | 25.08µl | 25 x 42 | 49 | $72.00 | |
| 72186-44 | .75 | 26.27µl | 25 x 44 | 47 | $72.00 | |
| 72186-54 | .75 | 32.25µL | 25 x 54 | 38 | $72.00 | |
| 72186-60 | .75 | 35.83µL | 25 x 60 | 35 | $72.00 | |
B. mSeries Lifterslip™
Solving the problem of hybridization non-uniformity
mSeries LifterSlip™ are manufactured from 1mm thick glass specially drawn for flatness and superior surface quality. The 1mm thickness has increased stiffness that reduces variation in hybridization fluid volume. Precise ink printing provides an innovative raised edge design for consistent dispersal and easy injection of hybridization solution under the prepositioned lifterslip.
mSeries Lifterslip™ Features:
- 1mm thick.
- Uniform disposal of hybridization solution.
- Reduced hybridization fluid volume variations.
- Superior flatness - substrate does not flex or bow.
- Precision cut edges.
- Superior surface quality.
- Consistent flatness.
Ordering:
| Catalog | Size | Aprox. volume | Pieces / box | Price | |
| 72181-25 | 22 x 25mm | 20µl | 50/bx | $78.00 |  |
| 72181-22 | 22 x 22mm | 17µl | 50/bx | $78.00 | |
| 72181-40 | 22 x 40mm | 29µl | 50/bx | $78.00 | |
| 72181-50 | 22 x 50mm | 36µl | 50/bx | $78.00 | |
| 72181-60 | 22 x 60mm | 45µl | 50/bx | $78.00 | |
| 72182-30 | 24 x 30mm | 24µl | 50/bx | $78.00 | |
| 72182-45 | 24 x 45mm | 33µl | 50/bx | $78.00 | |
| 72182-60 | 24 x 60mm | 47µl | 50/bx | $78.00 | |
| 72183-25 | 25 x 25mm | 23µl | 50/bx | $78.00 | |
| 72183-60 | 25 x 60mm | 55µl | 50/bx | $78.00 | |
| 72183-75 | 25 x 75mm | 58µl | 50/bx | $78.00 | |
mBox® in
Microarray
Slide Handling
Using mBox® – microarray handling without removing slides!
- Immerse 20 slides in liquid during blocking, denaturating, and post-arraying.
- It separates and prevents slide damage during shipping and processing.
- Optimally designed rack spacing for easy slide removal.
- Fits standard microtiter plate formats for centrifuging.
- Manufactured to prevent slide contamination.
- A convenient removable handle available.
Technical
mBox withstands boiling water without softening| Material: | Polypropylene |
| Maximum continuous use temperature: | 220°F (104°C) |
| Melting point: | 334°F (168°C) |
| Measures | 5” L x 31⁄2”W x 11⁄2” H (127mm x 89mm x 38mm) |
mHandle™
A removable stainless steel handle for the mBox®. Conveniently snaps‘on and off’ as needed
mTub™
Designed to fit the mBox®, the disposable tray for immersing or washing microscope slides. The mTub accepts most glass and stainless steel racks. mTub™ inside measurements are : 5” L x 3 1⁄2” W x 2 1⁄4” Deep. Capacity is 500 ml of solution. Packaging: 10 mTub™ per sleeve
Ordering:
| 72188-01 | The mBox® | each | $14.00 | |
| 72188-50 | mHandle™ | each | $26.00 | |
| 72188-60 | mTub™ | 10/ sleeve | $42.00 | |
Premium Substrate
for Microarray™
Premium substrates for microarray™ is used on a standard microscope slide 1 x3”, selective glass. All of the following slides are pre-packaged in a secure, no-contact mBox™, then vacuum-sealed in a foil barrier bag prior to shipping. Each package contains 20 slides.
UltraClean™ Glass Slide
A premium substrate ready to coat with your chemistry.
UltraClean™ substrate provides users with a pristine glass surface which can be used in any coating process compatible with glass such as silanization and polylysine. Manufactured from the highest quality low fluorescence glass.
UltraClean substrates are manufactured in a precise and controlled process incorporating HEPA filtration. This ensures a clean and consistent surface for optimum performance in microarray applications.
Options:
- Slides are available with ES – Enhanced Surface Treatment. ES Surface treatments produce a smaller, more uniform spot size without having to change your protocol
- Bar Coding – Ability to print all standard bar code formats
Glass Specifications:
- Flatness is <10μ variation over a 76mm diagonal interval
- 60/40 scratch-dig surface quality
- Soda-lime silicate glass
- 0.98 – 1.02 mm thickness
- Edges: plain cut
- Standard size: 25.10 x 75.36 ±0.38 mm
- Thickness: 1.00 ±0.02 mm
- Maximum edge chip: 0.05 mm
- Coating: none
- Printable area: 20 x 70 mm
| 63472-UC | UltraClean Slide | 20/bx | $180.00 | |
| 63472-BC | UltraClean Slide with Barcode | 20/bx | $175.00 | |
| 63472-ES | UltraClean Slide with ES Coating | 20/bx | $215.00 | |
2. DS8 Glass Slide
DS8 is a proprietary coating that allows for the spotting of compounds (RNA, etc.) followed by the transfection of the compounds into cells. Produced with proprietary Microarray Substrate, it provides the researcher with an appropriate surface for subsequent transfection studies and gene-expression work.
Options:
- Slides are available with ES – Enhanced Surface Treatment. ES
Surface treatments produce a smaller, more uniform spot size without
having to change your protocol.
Glass Specifications:
- Soda-lime silicate glass
- Edges: plain cut
- Standard size: 25.10 x 75.16 ±0.38mm
- Thickness: 1.00 ±0.02 mm
- Coating: DS8
- Printable area: 20 x 70 mm
| 63473-DS | DS8 Glass Slide | 20/bx | $305.00 | |
| 63473-ES | DS8 Glass Slide with ES Coating | 20/bx | $335.00 | |
3. Aminopropylsilane
Coated Substrates
SuperChip™ Aminopropylsilane coated substrates are designed for microarrays fabricated with the PCR products as probes. Manufactured from the highest quality, low fluorescence glass, SuperChip features a consistent, superior surface coating to provide a uniform DNA immobilizing surface. The result is the slide that binds an optimal amount of probe while minimizing nonspecific binding of labeled targets assuring high density of reactive groups and eliminating virtually all surface artifacts.
These SuperChip substrates are compatible with commercial microarray spotting and detection system. The SuperChip cleaning and coating process is tightly controlled in a HEPA filtered environment. Quality control includes contact angle and background fluorescence measurement of representative samples.
Glass Specifications:
- Edges: plain cut
- 60/40 scratch-dig surface quality
- Contact angle: 40° ± 5°
- Standard size: 25.10 x 75.36 ±0.38 mm
- Thickness: 1.00 – 0.02 mm
- Flatness is <10μm variable across a diagonal of 21mm x 75mm slide
- Thickness: 1.00 ±0.02 mm
- Maximum edge chip: 0.5 mm
- Coating: Aminopropylenesilane
- Printable area: 20 x 70 mm
| 63475-AP | Aminopropylsilane (APS) Slide | 20/bx | $300.00 | |
| 63475-BC | Aminopropylsilane (APS) Slide, Barcode | 20/bx | $330.00 | |
| 63475-ES | minopropylsilane (APS) Slide, ES | 20/bx | $358.00 | |
4. Aldehyde Silane Coated Substrates
Offers a uniform coating of aldehydes for attaching amine-modified DNA
| 63476-AS | Aldehyde / Silane Slide | 20/bx | $350.00 | |
| 63476-ES | Aldehyde / Silane Slide, ES | 20/bx | $385.00 | |
| 63476-EB | Aldehyde/Silane Slide, ES /Barcode | 20/bx | $380.00 | |
5. Epoxy Silane Coated Substrate
A uniform coating of epoxide groups for attaching amine modified DNA with an amine end. This is a vapor-coating process on UltraClean™ slides, which yields a uniform surface free from impurities (as a conventional liquid dip processes)
| 63477-AS | Epoxy / Silane Slide | 20/bx | $325.00 | |
| 63477-ES | Epoxy / Silane Slide, ES Glass | 20/bx | $350.00 | |
6. Poly-L-Lysine Coated Substrates
Using premium select, low fluorescence glass with a consistent uniform coating of poly-L-lysine these slides yield a dense layer of amine groups for initial ionic attachment of the negatively charged phosphate groups in the DNA backbone.
The DNA can subsequently be attached covalently to the slide by either baking or by UV irradiation. Our aging and packaging techniques ensure a product with a shelf life in excess of 6 months. Each lot is tested for background uniformity prior to shipping. The slides arrive ready for spotting – no pre-washing required.
| 63478-AS | Poly-L-Lysine Slide | 20/bx | $154.00 | |
| 63478-EB | Poly-L-Lysine Slide, ES /Barcode | 20/bx | $160.00 | |
7. Gold Coated Substrates
BioGold® substrates offer a high level of microaray performance through the use of a specialized thin-filmed gold coating. This specialized gold coating improves the adhesion of biological active cells to the substrate while its background reflective properties provide significant optical advantages for the detection of each cell’s unique light-wave signature.
Glass Specifications:
- Glass Type: Soda Lime White Glass
- Dimensions: (2.992” ±0.005”) x (0.984” ±0.040”) x 1mm Thick
- Edges: Plain Cut
- Gold Purity: 99.999%
- Coating Application: Vapor Deposition
- Thickness of Coating: 1200 Å ±20%
- Reflective properties: 83%
| 63479-AS | Gold-Coated Substrates | 20/bx | $180.00 | |
NUNC™ MaxiSorp™ 96 Well Plates
NUNC MaxiSorp plates have been the certified* ELISA plate industry standard for years. This same surface now sets a new standard for protein multiplexing.
- Hydrophilic MaxiSorp™ surface binds antibodies, protein and biomolecules.
- Flat bottom, solid polystyrene plates.
- Excellent for chemiluminescent, fluorescent and luminescent arrays.
- Standard plate footprint is compatible with exiting readers.
| Catalog # | 63494-CL | 63494-BK | 63494-WH |
| NUNC # | 439454 | 437111 | 436110 |
| Plate Material | Polystyrene | Polystyrene | Polystyrene |
| Color | Clear | Black | White |
| Key Applications | Protein & antibody multiplexing, chemiluminescence | Protein & antibody multiplexing, fluorescence | Protein & antibody multiplexing, luminescence |
| Method of attachment | Hydrophilic | Hydrophilic | Hydrophilic |
| Unit per Package /case | 5/60 | 10/80 | 10/80 |
| Price | $227.00 | $309.00 | $309.00 |
| Purchase | |
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*Certified for absorption of IgG; CV of <5% between wells; all results are ±10% from the mean and background of all well ±0.005 absorbance units from the mean
NUNC™ 4-Well Slide
Processing Dish
- Holds four 25 x 75 mm slides.
- Sterile.
- Clear polystyrene dish and lid.
| Catalog # | 63496 |
| NUNC # | 267061 |
| Plate Material | Polystyrene |
| Color | Clear |
| Unit per Package /case | 10/100 |
| Price | $50.00 |
| Purchase | |
NUNC
384-Well
Plate
- Natural polypropylene.
- Non-sterile.
| Catalog # | 63498-LV | 63498-ST | 63498-DW |
| NUNC # | 267459 | 264573 | 269390 |
| Description | Low Volume | Standard | Deep Well |
| Max Volume, μl/well | 58 | 120 | 252 |
| Working Volume, μl/well | 2 - 35 | 10 – 100 | 5 - 240 |
| Unit per Pack/Case | 25/100 | 20/120 | 5/60 |
| Price | $540.00 | $590.00 | $369.00 |
| Purchase | |
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Pronto™ Epoxide Slide
Spotting Solution
The Microarray spotting solution is optimized for printing
short oligonucleotides (~30 mers) providing excellent
spot morphology, extremely low background, and
minimal evaporation when used in conjunction with
above Epoxide Slides (#63477-series). Higher density
arrays can be printed due to the smaller spot size achieved using the
spotting solution
Pronto is a Trade Mark of Corning
| 63500-25 | Pronto™ Spotting Solution | 250 ml | $325.00 | |
Pronto™ Universal Slide
Spotting Solution
The Microarray spotting solution is optimized for printing both long oligonucleotides (>50 mers) and cDNA providing excellent spot morphology, extremely low background, and minimal evaporation when used in conjunction with Aminopropylsilane Slides (#63475- series). The hygroscopic formulation minimizes evaporation to < 5% after four hours of exposure allowing more arrays to be printed from the same source plate during extended printing runs.
Pronto is Trade Mark of Corning
| 63501-25 | Pronto™ Spotting Solution | 250 ml | $325.00 | |
PTFE
Printed Slides
PTFE Printed Slides, are extremely hydrophobic, acetone proof, chemical resistant and autoclavable. They are perfect for controlling cross-contamination in staining, immunolabeling and immunofluorescence tests. All printed slides are precleaned. The well surface is 100% wettable, which enhances cell attachment and increases its spreading capabilities within the well. These slides minimize the use of expensive purified antigens and antibodies. The slides measure 3x1" (76x26x1mm).
EMS Printed Slides are manufactured to order offering maximum shelf life. The shelf life of PTFE Printed Slides range from 18 months to two years. However, it is recommended that all glass slides (Printed or Un-printed) be used in a timely manner to avoid glass "weathering", (slides becoming cloudy or hazy even with proper storage. All of our Printed Slides are autoclavable and have a frosted end. They are intended for one time use only.
Features:
- Super Hydrophobic Mask
- Clean, Wettable Wells
- A Bioadhesive Surface Treatment to Enhance Cell and Tissue Attachment