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Electron Microscopy Sciences

Specimen Preparation and Embedding Supplies

arrow13Microarraying

Nitrocellulose-coated (NC) slides for protein microarrays,tdsarrow11ONCYTE® Film Slides

Nitrocellulose-coated (NC) slides for protein microarrays, cell lysate arrays, tissue printing and other applications

ONCYTE® is a three-dimensional microarray comprised of a combination of nitrocellulose polymer and proprietary chemistry that provides increased protein affinity and binding. It is also provides enhanced fluorescent chemiluminescent, radiographic, colormetric and visual signal compared to glass or other coated glass surfaces.

We provide nitrocellulose-coated products that includes anything from standards 25 mm x 75 mm glass microscope slides to SBS microtiter plate substrates in standard and custom formats.

Nitrocellulose film excels in various applications including:

  • Protein Microarrays
  • Immuno-arrays
  • Analyte Microarrays
  • Cell Lysate Arrays
  • Antibody Arrays
  • Tissue Printing

Our nitrocellulose film surfaces are manufactured and quality tested to the most exacting standards. To provide an optimal balance between binding capacity and fluorescence sensitivity, we offer two variants of nitrocellulose coatings.

The ONCYTE® AVID™ nitrocellulose-coated substrates are for applications that require the highest possible protein binding affinity and capacity. Therefore AVID™ slides are characterized by unparalleled protein binding capacity and affinity, making them ideal for colometric and infrared applications that require extremely high levels of binding.

The ONCYTE® NOVA™ nitrocellulose-coated substrates are recommended for applications that require the greatest fluorescent sensitivity but can accept reduced binding capacity. Therefore NOVA™ slides extremely low background fluorescence and an elegant, clean surface make them the most sensitive slides available for fluorescence – dependent arrays.
For Glass Slides (25 x 75 x 1mm):
EMS # Type Pack Price Select Specifications
70335-50A AVID™ 20 455.00 Cart ONCYTE Film Slide Specifications
70335-50N NOVA™ 20 455.00 Cart
1 - 20 x 51mm NC pad per slide
70335-51A AVID™ 20 520.00 Cart ONCYTE Film Slide Specifications
70335-51N NOVA™ 20 520.00 Cart
1 - 20 x 60mm NC pad per slide
70335-52A AVID™ 20 520.00 Cart ONCYTE Film Slide Specifications
70335-52N NOVA™ 20 520.00 Cart
1 - 21 x 71mm NC pad per slide
70335-53A AVID™ 20 563.00 Cart ONCYTE Film Slide Specifications
70335-53N NOVA™ 20 563.00 Cart
2 - 20 x 20mm NC pad per slid
70335-54A AVID™ 20 563.00 Cart ONCYTE Film Slide Specifications
70335-54N NOVA™ 20 563.00 Cart
8 - 6 x 6mm NC pad per slide
70335-55A AVID™ 20 606.00 Cart ONCYTE Film Slide Specifications
70335-55N NOVA™ 20 606.00 Cart
12 - 6 x 6mm NC pad per slide
70335-56A AVID™ 20 577.00 Cart
70335-56N NOVA™ 20 577.00 Cart
16 - 6 x 6mm NC pad per slide




70335-57A AVID™ 20 693.00 Cart ONCYTE Film Slide Specifications
70335-57N NOVA™ 20 693.00 Cart
16 - 6.5 x 6.5mm NC pad per slide



70335-58A AVID™ 20 757.00 Cart ONCYTE Film Slide Specifications
70335-58N NOVA™ 20 757.00 Cart
64 - 2.5 x 2.5mm NC pad per slide



70335-59A AVID™ 20 563.00 Cart ONCYTE Film Slide Specifications
70335-59N NOVA™ 20 563.00 Cart
1 - 15 x 69mm NC pad per slide



70335-60A AVID™ 20 563.00 Cart ONCYTE Film Slide Specifications
70335-60N NOVA™ 20 563.00 Cart
2 - 25 x 32mm NC pad per slide




70335-61A AVID™ 20 563.00 Cart ONCYTE Film Slide Specifications
70335-61N NOVA™ 20 563.00 Cart
4 - 15 x 15mm NC pad per slide




70335-62A AVID™ 20 693.00 Cart ONCYTE Film Slide Specifications
70335-62N NOVA™ 20 693.00 Cart
8 - 6 x 15mm NC pad per slide




70335-63A AVID™ 20 649.00 Cart ONCYTE Film Slide Specifications
70335-63N NOVA™ 20 693.00 Cart
8 - 6 x 20mm NC pad per slide




For SBS Compliant Microtiter Plates with Glass Substrate:
EMS # Type Pack Price    
70335-70A AVID™ each 184.00 Cart ONCYTE Film Slide Specifications
70335-70N NOVA™ each 184.00 Cart
96 NC Round Pads 6mm diameter










70335-71A AVID™ each 184.00 Cart ONCYTE Film Slide Specifications
70335-71N NOVA™ each 184.00 Cart
96 NC Square Pads 6 x 6mm








70335-72A AVID™ each 209.00 Cart ONCYTE Film Slide Specifications
70335-72N NOVA™ each 209.00 Cart
384 NC Square Pads 2.5 x 2.5mm







ONCYTE® Barcode Formats:
EMS # Description Pack Price Select  
70335-BF1 20 x 2.5 mm each 1.26 Add to Cart ONCYTE® Barcode Formats
70335-BF2 20 x 7 mm each 1.26 Add to Cart ONCYTE® Barcode Formats
70335-BF2 22 x 14mm each 1.26 Add to Cart ONCYTE® Barcode Formats

References:

  1. High Definition Cell Analysis IN Situ Using Microporous Films. Cel Vision 2:499 – 599, 1995.
  2. Cytometrically Coherent Transfer of Receptor Proteins on Microporous Membranes. BioTechnique 20:641-650. 1991.

Film Well™arrow11Film Well™

Slide based low volume, removable chambers for protein binding.

Slide based low volume, removable chambers for protein bindingA combination of our SecureSeal™ chambers and ONCYTE® nitrocellulose film coated slides that provide an easily removable, low volume chamber and the protein binding benefits of a nitrocellulose coating.

ONCYTE® is a 3-dimensional microarray substrate bound to a standard 25mm x 75mm glass slide. A combination of nitrocellulose polymer and proprietary chemistry maximize protein binding and stability. The film is compatible with virtually all detection systems, including fluorescent, chemiluminescent, radiographic and colorimetric.

Please refer to the SecureSeal™ chambers (Click here for details) and ONCYTE® Film Slides (Click here for details) pages for pricing and ordering information.

ProPlate Multi-Array Slide SystemEliSpot™ Coated Glass Slidesarrow11ProPlate™ MicroArray System

ProPlate™ Multi-Array Slide System
Integration of microplate and biochip technologies

ProPlate™ integrates microscope slide based array technology with automated microtiter plate processing. Up to 64 individual arrays can be assayed per slide. Four slides fit into a tray, producing a modular plate with a standard microtiter plate footprint and well spacing allowing automated robotic processing of up to 256 arrays.

  • ProPlate Tray SetUp to 64 leak-proof wells on any slide surface without the use of adhesive
  • Microtiter plate well spacing facilitates use with multichannel pipettes
  • 7 x 7 mm square well are ideal for high content arrays
  • 5 – 350 µl well volumes accommodate small reagent and large wash volumes
  • Available in Chip Chip™ compatible formats.

Ordering:

SBS Compliant Tray Sets

Each ProPlate Tray Set includes: 4 Slide modules, 8 Snap clips, 1 Tray and Cover, 10 seal strips and one applicator.
Tray conforms to SBS standards for footprint (81.7mm width x 123.7mm length) and well spacing.

EMS # Description Qty Price  
63484-01 1 Well ProPlate Tray set
3 – 16.5 x 70 mm Well, Stainless Steel Spring Clips, Tray, Cover, 10 Seal Strips and 1 Seal Strip applicator
each 205.00 Add to Cart
63484-02 Code #246882. 2 Well ProPlate Tray set
3 – 16.5 x 34 mm, 2 Well Modules, Stainless Steel Spring Clips, Tray, Cover, 10 Seal Strips and 1 Seal Strip applicator
each 140.00 Add to Cart
63484-03 3 Well ProPlate Tray set
3 – 18 x 20 mm, 3 Well Modules, Stainless Steel Spring Clips, Tray, Cover, 10 Seal Strips and 1 Seal Strip applicator
each 205.00 Add to Cart
63484-04 4 Well ProPlate Tray set
3 – 16 x 16 mm, 4 Well Modules, Stainless Steel Spring Clips, Tray, Cover, 10 Seal Strips and 1 Seal Strip applicator
each 205.00 Add to Cart
63484-05 8 Well ProPlate Tray set
3 – 7 x 16 mm, 8 Well Slide Modules, Stainless Steel Spring Clips, Tray, Cover, 10 Seal Strips and 1 Seal Strip applicator
each 205.00 Add to Cart
63484-06 16 Well ProPlate Tray set
3 – 6 mm Diameter, 16 Well Slide Modules, Stainless Steel Spring Clips, Tray, Cover, 10 Seal Strips and 1 Seal Strip applicator
each 140.00 Add to Cart
63484-07 16 Well ProPlate Tray set
3 – 7 x 7 mm, 16 Well, Stainless Steel Spring Clips, Tray, Cover, 10 Seal Strips and 1 Seal Strip applicator
each 205.00 Add to Cart
63484-08 64 Well ProPlate Tray set
3 – 3.5 x 3.5 mm, 64 Well Slide Modules, Stainless Steel Spring Clips, Tray, Cover, 10 Seal Strips and 1 Seal Strip applicator
each 205.00 Add to Cart
63484-09 1 Well ProPlate Tray set
4 – 16.5 x 70 mm Well Modules, Delrin Snap Clips, Tray, Cover, 10 Seal Strips and 1 Seal Strip applicator
each 225.00 Add to Cart
63484-10 2 Well ProPlate Tray set
4 – 16.5 x 34 mm, 2 Well Modules, Delrin Snap Clips, Tray, Cover, 10 Seal Strips and 1 Seal Strip applicator
each 190.00 Add to Cart
63484-11 3 Well ProPlate Tray set
4 – 18 x 20 mm, 3 Well Modules, Delrin Snap Clips, Tray, Cover, 10 Seal Strips and 1 Seal Strip applicator
each 210.00 Add to Cart
63484-12 4 Well ProPlate Tray set
4 – 16 x 16 mm, 4 Well Modules, Delrin Snap Clips, Tray, Cover, 10 Seal Strips and 1 Seal Strip applicator
each 210.00 Add to Cart
63484-13 8 Well ProPlate Tray set
4 – 7 x 16 mm, 8 Well Slide Modules, Delrin Snap Clips, Tray, Cover, 10 Seal Strips and 1 Seal Strip applicator
each 210.00 Add to Cart
63484-14 16 Well ProPlate Tray set
4 – 6 mm Diameter, 16 Well Slide Modules, Delrin Snap Clips, Tray, Cover, 10 Seal Strips and 1 Seal Strip applicator
each 140.00 Add to Cart
63484-15 16 Well ProPlate Tray set
4 – 7 x 7 mm, 16 Well Slide Modules, Delrin Snap Clips, Tray, Cover, 10 Seal Strips and 1 Seal Strip applicator
each 210.00 Add to Cart
63484-16 64 Well ProPlate Tray set
4 – 3.5 x 3.5 mm, 64 Well Slide Modules, Delrin Snap Clips, Tray, Cover, 10 Seal Strips and 1 Seal Strip applicator
each 210.00 Add to Cart
Slide Modules
EMS # Clip Qty Price Select Specifications
63484-21  - 10 198.00 Cart ProPlate Multi-Array Slide System Specifications
63484-31 Delrin 2 58.00 Cart
63484-41 SS 2 58.00 Cart
1 Well ProPlate Module
16.5 x 70 mm Well






63484-22 - 10 198.00 Cart ProPlate Multi-Array Slide System Specifications
63484-32 Delrin 2 58.00 Cart
63484-42 SS 2 58.00 Cart
2 Well ProPlate Module
16.5 x 34 mm Well







63484-23 - 10 198.00 Cart ProPlate Multi-Array Slide System Specifications
63484-33 Delrin 2 58.00 Cart
63484-43 SS 2 58.00 Cart
3 Well ProPlate Module
18 x 20 mm Well







63484-24 - 10 198.00 Cart ProPlate Multi-Array Slide System Specifications
63484-34 Delrin 2 58.00 Cart
63484-44 SS 2 58.00 Cart
4 Well ProPlate Module
16 x 16 mm Well






63484-25 - 10 198.00 Cart ProPlate Multi-Array Slide System Specifications
63484-35 Delrin 2 58.00 Cart
63484-45 SS 2 58.00 Cart
8 Well ProPlate Module
7 x 16 mm Well







63484-26 - 10 198.00 Cart alt=
63484-36 Delrin 2 58.00 Cart
63484-46 SS 2 58.00 Cart
16 Well ProPlate Module
6 mm Diameter Well
10 per pack




63484-27 - 10 62.00 Cart 16 Well ProPlate Module Specifications
63484-37 Delrin 2 58.00 Cart
63484-47 SS 2 75.00 Cart
16 Well ProPlate Module-
7 x 7 mm Well


63484-28 - 10 225.00 Cart ProPlate Multi-Array Slide System Specifications
63484-38 Delrin 2 75.00 Cart
63484-48 SS 2 66.00 Cart
64 Well ProPlate Module
3.5 x 3.5 mm Well






ProPlate Silicone Gasket only
EMS #     Qty Price  
63484-51 1 Well 16.5 x 70 mm 10 50.00 Add to Cart
63484-52 2 Well 16.5 x 24 mm 10 50.00 Add to Cart
63484-53 3 Well 16 x 20 mm 10 50.00 Add to Cart
63484-54 4 Well 16 x 16 mm 10 80.00 Add to Cart
63484-55 8 Well 7 x 16 mm 10 77.00 Add to Cart
63484-56 16 Well 6 mm Diameter 10 77.00 Add to Cart
63484-57 16 Well 7 x 7 mm 10 77.00 Add to Cart
63484-58 64 Well 3.5 x 3.5 mm 10 105.00 Add to Cart
Accessories
EMS # Qty Price    
63484-80 50 52.00 Cart Seal Strips and Seal Strip Applicator
Seal Strips (50) and Seal Strip Applicator
63484-81 20 47.00 Cart ProPlate Delrin, Non-Numbered Snap Clips
63484-82 20 47.00 Cart ProPlate Stainless Steel Spring Clips
ProPlate Stainless Steel  Spring Clips
63484-83 each 79.00 Cart ProPlate Tray and Cover for Snap Clip Modules
Holds 4 Slide Modules
63484-84 each 79.00 Cart ProPlate Tray and Cover for Spring Clip Modules
Holds 3 Slide Modules

ProPlate Microtiter Platearrow11ProPlate Microtiter Plate (MP)

Patented  ProPlate™ technology for high throughput robotic processing of microarrays

Grace BioLabs developed bottomless SBS compliant microtiter plate superstructures for use with robotic processors. TheSuperstructures come in a permanently affixed well format – the ProPlate MP™, or a removable well format – the FlexPlate MP™.

The ProPlate MP™ SBS compliant microtiter plate features a SecureSeal™ Plus adhesive bonding system applied to a bottomless 96-round well, black, polystyrene microtiter plate. Simply peel off the protective polyliner and apply large format array for automated processing.

The proprietary adhesive forms a water-tight seal between a microarray substrate and the bottomless microtiter plate. Grace Bio Labs offers plate with SBS standard spacing in both 96-well (shown) and 384-well formats.

Key product features include:

  • Guaranteed flat SBS compliant microtiter plate format which enables robotic processing of assays
  • Water-tight seal between superstructure and substrate
  • Compatible with glass or plastic substrates.
  • Available in 96 round or square or 384 square well format
  • Available as a complete microtiter plate with microcellulose film coated substrate, uncoated substrate or as an adhesive superstructure alone.
  • Plates are available with SBS standard spacing in both 96-well and 384-well formats.

Ordering:

SBS Compliant Adhesive and Non-Adhesive Microtiter Plates
EMS # Description Qty Price  
63485-01 96 Square Well ProPlate
Adhesive SBS Microtiter Plate
Black Polystyrene
each 49.00 Add to Cart
63485-02 96 Round Well ProPlate
Adhesive SBS Microtiter Plate
Black Polystyrene
each 49.00 Add to Cart
63485-05 364 Square Well ProPlate
Adhesive SBS Microtiter Plate
Black Polystyrene
each 65.00 Add to Cart
63485-10 96 Square Well FlexPlate
7.25 x 7.25 mm
9 mm Center to Center Well Spacing
4.5 mm Depth
110 x 75 mm OD
Clear Silicone
Adhesive One Slide
each 52.00 Add to Cart
63485-11 96 Square Well FlexPlate
7.25 x 7.25 mm
9 mm Center to Center Well Spacing
4.5 mm Depth
110 x 75 mm OD
Clear Silicone
Adhesive One Slide
Black Polystyrene Frame
each 82.00 Add to Cart
SBS Compliant Microtiter Plates with ONCYTE® Nitrocellulose Coated Substrates
EMS # Description Qty Price  
63485-20 ONCYTE AVID® 96 NC Round Pads
6 mm Diameter for SBS Microtiter Plate
Glass Substrate
each 184.00 Add to Cart
63485-21 ONCYTE AVID® 96 NC Square Pads
6 x 6 mm  for SBS Microtiter Plate
Glass Substrate
each 184.00 Add to Cart
63485-22 ONCYTE AVID® 96 NC Round Pads
6 mm  Diameter for SBS Microtiter Plate
Plastic Substrate
each 99.00 Add to Cart
63485-23 ONCYTE AVID® 384 NC Square Pads
2.5 x 2.5 mm  for SBS Microtiter Plate
Glass Substrate
each 209.00 Add to Cart
63485-24 ONCYTE NOVA® 96 NC Round Pads
6 mm  Diameter for SBS Microtiter Plate
Glass Substrate
each 189.00 Add to Cart
63485-25 ONCYTE NOVA® 96 NC Square Pads
6 x 6 mm  for SBS Microtiter Plate
Glass Substrate
each 184.00 Add to Cart
63485-26 ONCYTE NOVA® 96 NC Round Pads
6 mm  Diameter for SBS Microtiter Plate
Plastic Substrate
each 99.00 Add to Cart
63485-27 ONCYTE NOVA® 384 NC Square Pads
2.5 x 2.5 mm  for SBS Microtiter Plate
Glass Substrate
each 209.00 Add to Cart

FlexWell Incubation Chambersarrow11FlexWell™ Incubation Chambers

FlexWell™ removable incubation chambers for microarrays using ONCYTE® slides

Features:

  • Silicone FlexWell™ forms well on slides using a clean release adhesive to isolate up to 16 specimens per slide
  • Slides and FlexWell™ fit into a reusable slide tray organizing wells into the footprint of a SBS compliant microtiter plate
  • Gasket may be sealed for mixing and to prevent evaporation
  • Available in eight-well and 16-well formats

Ordering:

EMS # Qty Price Select Description
63485-50 10 103.00 Cart FlexWell Incubation Chamber Specifications
FlexWell 8 – 6.5 x 6.5 mm Wells 3.2mm Depth
25 x 75 mm OD
Clear Silicone Adhesive one Side





63485-51 10 152.00 Cart
FlexWell 16 – 6.5 x 6.5 mm Wells
3.2mm Depth
25 x 75 mm OD
Clear Silicone Adhesive one Side



63485-52 10 115.00 Cart FlexWell Incubation Chamber Specifications
FlexWell 64 – 3.5 x 3.5 mm Wells
1.8mm Depth
25 x 75 mm OD
Clear Silicone Adhesive one Side


63485-53 50 51.00 Cart FlexWell Incubation Chamber Specifications
Seal Strips
24 mm x 77 mm Seal Area










63485-54 each 37.00 Cart FlexWell Incubation Chamber Specifications
4 Slide Tray Holds up to 4 Slides
Tray Measures 75.9 x 127.6 mm OD










63485-55 5 234.00 Cart FlexWell Incubation Chamber Specifications
FlexPlate 96 Square Well
7.25 x 7.25 mm
9 mm Center-to-Center Well Spacing
4 mm Depth
110 x 75 mm OD
Clear Silicone
Adhesive on Side
63485-56 each 86.00 Cart
With Black Polystyrene Frame


References

Microarraying Products References

Chambers for Microarrays

Versatile Hybridization Chambers for Genomic and Proteomic Arrays

  • Versatile sizes and styles
  • Ideal for high throughput screening of genomic and protein arrays on microscope slides
  • Designed to minimize reagent volume, eliminate evaporation and promote reagent mixing for uniform hybridization
  • Materials are nuclease free and temperature rersistant

HybriSlip™ Hybridization Coverslips (Click here for details)

  • Specially selected hydrophobic plastic covers do not bind probes to surfaces like glass coverslips
  • HybriSlips™ are lighter weight than glass, minimizing friction and facilitating uniform reagent distribution
  • HybriSlips™ are ready-to-use without pretreatment
  • Working surfaces are protected with clean release liners to prevent contaminations
  • HybriSlips™ remain flat and will not curl, even at high temperatures

HybriWell™ Hybridization System (Click here for details)

  • HybriWell™ seals securely to a microscope slide surface in seconds
  • Access ports in the chamber surface allow the addition of reagents
  • Ports are easily sealed using seal tabs provided with each order
  • Sealed chambers are water tight
  • The disposable chamber removes cleanly and easily even after heating.

SecureSeal™ Hybridization Chambers (Click here for details)

  • Gasketed chambers provide optimum surface volume fluid dynamics for hybridization assays.
  • Designed to minimize friction especially when active mixing is employed during assay
  • Partially filled chambers may be sealed and rotated continuously for superior mixing

FlexWell™ (Click here for details)

  • Silicone gasket forms wells on slides using clean release adhesive to isolate up to 16 specimens per slide
  • Slides fit into a reusable slide tray organizing wells into the footprint of a SMS compliant micrototer plate
  • Gasket may be sealed for mixing and to prevent evaporation. Available 8-wells and 16-wells.

Note:  HybriWell™ and SecureSeal™ are recommended for most protein and nucleic acid assays.  If you are using CY5® or Alexa Fluor® 647 direct-labeled DNA probes, please refer to our new “fluorescent friendly” chambers for DNA microarrays or click here for details.

Hybridization and Incubation arrow13arrow13