Wells, Optical Bottom Plates, Cell Culture Inserts

MicroWell® Plates

MicroWell Plates are used in a broad spectrum of applications, including cloning experiments, sample incubations, and in tissue culture based analytical systems. Our MicroWell Plates have excellent cell attachment. All styles are designed to be stackable and are radiation sterilized.

µ 10µl Well Plates

They are Terasaki format plates measuring 56x82mm and having 60 or 72 wells. Wells are shaped like inverted truncated cones, each with a working volume of 10µl. The well surface is Nunclon® treated for good cell attachment. Lids incorporate a stacking feature and are tight-fitting, which reduces evaporation.

µ 96 Well Plates

These plates measure 86x128mm and come in either flat or round bottom wells. Vented lids allow for gas exchange and have condensation rings to control evaporation and retain condensate. Lids are keyed so they cannot be accidentally reversed. Plates have alpha-numeric markings on its two edges. In addition, flat bottom plates have individual well coordinates beside each well for easy well identification when using an inverted microscope. Plates also have raised well rims to provide proper attachment of adhesive sealing film.

Number of Wells	Well Shape	Volume Well	Culture Area cm2	Unit Per Sleeve/cs
96	Round	0.3ml	0.36	1/50
96	Flat	0.4ml	0.328	1/50
60	Conical	10µl	0.013	10/150
72	Conical	10µl	0.013	10/150

70436-R1	96-Well, Round, Without Lid	10/pk	42.00
70436-R5	96-Well, Round, Without Lid	50/cs	149.00
70436-F1	96-Well, Flat, Without Lid	10/pk	43.00
70436-F5	96-Well, Flat, Without Lid	50/cs	174.00
70436-L1	Lid for 96-Well	10/pk	30.00
70436-L5	Lid for 96-Well	50/cs	109.00
70436-ST	Sealing Tape (80x134mm)	50/bx	39.00
70437-R1	96-Well, Round, With Lid	10/pk	60.00
70437-R5	96-Well, Round, With Lid	50/cs	214.00
70437-F1	96-Well, Flat, With Lid	10/pk	60.00
70437-F5	96-Well, Flat, With Lid	50/cs	214.00
70438-10	60-Well, Conical, With Lid	10/pk	27.00
70438-15	60-Well, Conical, With Lid	150/cs	196.00
70439-10	72-Well, Conical, With Lid	10/pk	27.00
70439-15	72-Well, Conical, With Lid	150/pk	196.00

Technical Tip

Permanox Plastic; Non Fluorescent

Well Orienter, Stand and Pad

For Easy Pipetting and Identification of Samples in Microplates

The Well Orienter

• Convenient visual aid for pipetting and
  identification of samples in microplates.
• Sharp, alpha-numeric characters
  compatible with 96-, 48- or 24-well
  microplates.
• No more cross-referencing headaches!

The Well Plate Stand

• View samples at a comfortable 30° angle and pipette without strain
• The neoprene pad and rubber feet prevents slippage of the plate and stand

The Well Pad

• A convenient note pad that corresponds to the layout of a 96-, 48- or 24-well plate
• Keep accurate records of samples in a microplate
• Adhere sheets into a notebook to create a quick reference guide

Ordering

70490-10	96 Well Orienter	4/pk	25.00
70490-11	48 Well Orienter	4/pk	25.00
70490-12	24 Well Orienter	4/pk	25.00
70490-20	Well Plate Stand	each	30.00
70490-30	96 Well Pad	50 sheets	10.00
70490-31	48 Well Pad	50 sheets	10.00
70490-32	24 Well Pad	50 sheets	10.00

96-well & 384-well Color Orienters

• Bright colors make it easy to distinguish between columns and rows
• Fits most standard flat and round bottom 96-well and 384-well plates
• Ideal for use with The Pipetter Guide for efficient and stress-free identification of rows and columns
  

70490-15	Multi-colored 384 Well Orienter	2/pk	30.00
70490-16	Multi-colored 384 Well Orienter	2/pk	30.00
70490-17	Multi-colored 96 Well Orienter	2/pk	30.00
70490-18	Multi-colored 96 Well Orienter	2/pk	30.00

Adjustable Well Plate Stand

A Convenient, Adjustable-Angle Stand for Pipetting

• Achieves accurate pipetting by allowing the flexibility to adjust the angle of the microplate
• Convenient knob easily adjusts the angle (45° max. tilt) required to fully view every well
• Compact design takes up little bench-top space measuring only 6˝ L x 4 1⁄2˝ W x 2 1⁄2˝ H
• Durable acrylic material will last for years if properly cared for Adjustable Well Plate Stand shown with a 96-well Color Orienter (sold separately)

70493-01

Adjustable Well Plate Stand

each

53.00

Well Orienter Stickers

• Permanently label mutliple microplates
• Wells are clearly marked with its corresponding row number and column letter for quick cross-referencing
• Scored paper backing makes aligning quick and easy

The markings of each filled well are easily readable.

70490-14

96-Well Orienter Stickers

50/pk

29.00

Cover Slips; Thermanox®; TMX Coverslips

NUNC™ Brand Thernanox®, or TMX coverslips are made from a polymer (in the polyolefin family) that is highly resistant to most chemicals. Thermanox® plastic is resistant to alcohols, aldehydes, hydrocarbons, dilute acids (<10%) and dilute alkalis (<2%). TMX has limited resistance to chlorinated hydro-carbons, however, it is not resistant to concentrated acids or bases.Thermanox® plastic is a flexible, trans-parent polymer that can be sectioned using a microtome and is able to withstand high temp-eratures (temperature range –70ºC to +150ºC). Thermanox® coverslips are culture treated on one side for enhanced cell attachment and growth. The treated side is packaged face up toward the label.

Features:

• Flat and remain flat
• 0.2mm (No. 2) thickness
• Resistant to commonly used solvents
• Safe and easy to handle
• Auto fluorescent in the range 380 to 545 nm
• Thernanox® cover slips can be autoclaved at 20 minutes steam, 20 minutes dry at 120°C

72274-01	10.5x22mm Thermanox	100/pk	62.00
72274-05	10.5x22mm Thermanox	500/bx	260.00
72270	22x60mm Thermanox	100/bx	62.00
72271	22x60mm Thermanox	500/bx	260.00
72272	24x30mm Thermanox	100/bx	62.00
72273	24x30mm Thermanox	500/bx	260.00
72280	13mm Round Thermanox	100/bx	62.00
72281	13mm Round Thermanox	500/bx	260.00
72275-01	15mm Round Thermanox	100/pk	62.00
72275-05	15mm Round Thermanox	500/bx	260.00
72282	22mm Round Thermanox	100/bx	62.00
72283	22mm Round Thermanox	500/bx	260.00
72285	25mm Round Thermanox	100/bx	62.00
72286	25mm Round Thermanox	500/bx	260.00

MultiDishes Nunclon® Surface

Multi Dish Plates are used in a variety of cell biology applications. They all offer excellent cell attachment and growth surface. A certificate of satisfaction comes with each order.

• Useful in all areas of cell culture, including assays and cloning
• Raised well rims to lower risk of cross contamination
• Optically clear and uniformity surface, suitable for microscopy
• Nunclon® certified for consistent cell growth

Number of Wells	Well Size mm	Suggested working Vol ml/well	Culture Area cm2/well	Plate size, mm	Units Bag/Case
4 Round	15	0.4-1.0	1.9	66x66	4/120
4 Rectangle	24x67	3.5-8.0	16	82x132	10/100
6 Round	35	2.0-5.0	9.6	88x132	5/85
8 Rectangle	24x33	2.0-4.5	8.6	82x132	10/100
12 Round	21 Dia.	0.7 – 1.8	3.5	86 x 128	1/75
24 Round	15	0.4-1.0	1.9	88x132	5/85
48 Round	12 Dia.	0.3 – 0.6	1.1	86 x 128	1/75

70425	176740	4-Well, Round Plates	28/bag	74.00
70426	176740	4-Well, Round Plates	120/cs	262.00
70428	150229	6-Well, Round Plates	15/bag	64.00
70429	150229	6-Well, Round Plates	85/cs	212.00
70424	150628	12-Well, Round Plates	75/pk	214.50
70427	150687	48-Well, Round Plates	75/pk	284.00

Optical Bottom Microwell® Plates

The NUNC™ Coverglass Optical Bottom 96- and 384- Well Plates have the same quality design of existing NUNC well products, plus an optically clear polystyrene plate bottom. This clear base permits microscopic viewing and reading of well content from the top, bottom or both.

The plate’s upper structure is offered in black (for fluorometric detection) or white (for luminescence detection).

1. 96 Well Optical Bottom Plates Polystyrene/Coverglass

Sterile. External dimensions 128 x 86 mm.

Features:

• White or black upper structure with #1.5 borosilicate coverglass
• Minimum light scatter and low autofluorescence
• Optical clarity for viewing well contents
• Flat bottom well geometry for plate reader access
• Footprint compatible with standard equipment and automated systems
• CC2™ surface modification closely mimics Poly-D-Lysine and it superior for attachment and growth of fastidious cells
• Non-treated plates are optimized for fluorescence
• Sterile for cell culture
• Working range: 50 – 200 μl/well

Plate total height	14.9 mm
H2 Vertical distance from inside well bottom to resting plane.	2.5 mm
H3 Inside depth of well	12.2 mm
H4 Coverglass thickness	0.19 mm
H5 Height of flange	6.2 mm
Well diameter	6.5 mm
Total Vol. μl/well	400

Cat. #	NUNC #	Surface	Color	Sterile	Lid	Units per pack/case
64815-04	164 588	Cell Culture	Black	Yes	Yes	6/pk	224.00
64815-05	164 588	Cell Culture	Black	Yes	Yes	6/30	1118.00
64815-09	164 590	Cell Culture	White	Yes	Yes	6/pk	224.00
64815-10	164 590	Cell Culture	White	Yes	Yes	6/30	1118.00
64815-15	265 300	Non-treated	Black	No	No	5/30	1010.00
64815-20	160 376	CC2	Black	Yes	Yes	6/30	1535.00

96 –Polymer Base

External dimension: 128 x 86 mm

• White or black upper structure with Polymer bottom combines the optical clarity of virgin crystalline polystyrene with optimal surface for a wide range of HTS applications
• Optical clarity for viewing well contents
• Footprint compatible with standard equipment and automated systems
• Flat bottom well geometry for plate reader access
• Cell culture, poly-D-Lysine or Collagen I surfaces for cell-based array
• Non-treated plates are optimized for scintillation counting
• Working ranges: 50 –200 μl/well

Plate total height	14.9 mm
H2 Vertical distance from inside well bottom to resting plane.	2.55 mm
H3 Inside depth of well	12.2 mm
H4 Polystyrene film	0.25
H5 Height of flange	6.2 mm
Well diameter	6.5 mm
Total Vol. μl/well	400

Cat. #	NUNC #	Description	Color	Sterile	Lid	Units /case
64810-05	165 306	Cell Culture	White	yes	yes	10/30	210.00
64810-15	165 305	Cell Culture	Black	yes	yes	10/30	210.00
64810-16	152 036	Collagen I	Black	no	yes	5/20	323.00
64610-17	152 040	Collagen I	White	no	yes	5/20	323.00
64810-18	152 028	Poly-D-Lysine	White	no	yes	5/20	311.00
64810-19	152 037	Poly-D-Lysine	Black	no	yes	5/20	311.00
64811-10	265 301	Non-treated	Black	no	no	10/30	150.00
64811-05	265 302	Non-treated	White	no	no	10/30	150.00

Lid for the above 96 Well Optical Bottom Plates

Polystyrene
External dimensions 128 x 86 mm

Cat. #	NUNC #	Description	Color	Sterile	Units /sleeve
64811-50	255 983	96 Well Optical Bottom Plates Lid	clear	No	20/60	80.00
64811-51	235 304	96 Well Optical Bottom Plates Lid	Clear	Yes	20/60	90.00

3. 384 Well® Optical Bottom Plates – Polymer Base

• White or black upper structure with optical clear polystyrene bottom
• Can be read from top or bottom
• Rounded square wells eliminate wicking
• Optimized for scintillation counting
• Cell Culture, Collagen I or Poly-D-Lysine surfaces for cell-based arrays
• Non-treated surface for homogeneous assays
• Working range: 10 – 100 μl/well

H1 Plate total height	14.4mm
H2 Vertical distance from inside well bottom to resting plane	2.7mm
H3 Inside depth of well	11.6mm
H4 Polystyrene film	2.5mm
H5 Height of flange	2.7mm
H7 Bottom Thickness	0.3mm
D1 Well diameter, top	3.7 x 3.7mm
D2 Well distance bottom	2.9 x 2.9mm
F1 Depth of flange	1.8mm
F1 Depth of flange	1.8mm

Ordering: External plate dimension 128 x 86 mm

Cat. #	NUNC #	Description	Color	Sterile	Lid	Units /case
64810-25	142762	Cell Culture	White	yes	yes	10/30	315.00
64810-35	142761	Cell Culture	Black	yes	yes	10/30	30415
64810-40	164730	Cell Culture	Black	yes	no	10/30	278.00
64810-50	152032	Collagen I	White	no	yes	5/20	283.00
64810-52	152041	Collagen I	Black	no	yes	5/20	486.00
64810-54	152029	Poly-D-Lysine	Black	no	yes	5/20	486.00
64810-56	152033	Poly-D-Lysine	White	no	yes	5/20	486.00
64811-15	242763	Non-treated	White	no	no	10/30	293.00
64811-20	242764	Non-treated	Black	no	no	10/30	293.00

* Certified

Sealing Films

Pre-Cut-Adhesive Films For Microplate Sealing Needs

Sterile –Sterilized by gamma irradiation. Films are also free of cytotoxins.

Prevent Contamination – Films eliminate well-to-well contamination, microbial and viral contamination, evaporation (excluding breathable membranes) and sample spillage.

Ideally Sized and Designed – 6" x 3.25" rectangles fit all microplates. Two scored end-tabs make film positioning and removal hassle-free.

Thermal Stability – Designed to withstand a wide temperature range.

Easy Specimen Retrieval – Pierceable sealing films facilitate easy removal of samples with a pipet tip.

Titer-Tops

Film Type	Titer-Tops®
Ideal Use	Misc Storage
Temp. Range	-40°C to 100°C
Thickness	4.4 mil (0.0044 in.)
Material	Polyurethane
Gas-Permeable	No
UV Transparent	To 250nm

70538-05	Titer-Tops, 6" x 3.25"	50/pk	40.00
70538-10	Titer-Tops, 6" x 3.25"	100/pk	65.00

Breathe-Easy®

An Adhesive, Gas-Permeable, Cell Culture Membrane

Film Type	Breathe-Easy*
Ideal Use	Cell Culture
Temp. Range	-80°C to 100°C
Thickness	1 mil (0.001 in.)
Material	Polyurethane
Gas-Permeable	Yes
UV Transparent	To 300nm

 

70536-10

Breathe-Easy, 100/pk

100/pk

73.00

Breathe-EASIER™

A Rapid Gas Transmissable Sealing Membrane for Cell Growth. Breathe-Easier membranes permit rapid, consistent gas exchange of Oxygen, CO2 and Water Vapor for uniform cell growth.

Film Type	Breathe-Easier™
Ideal Use	Cell Culture
Temp. Range	-20°C to 80°C
Thickness	5.5 mil (0.0055 in.)
Material	Non-woven rayon
Gas-Permeable	Yes
UV Transparent	No

70536-20

Breathe-EASIER

100/pk

60.00

AluminaSeal™

Pre-Cut Adhesive Foil Sealing Film

Film Type	AluminaSeal™
Ideal Use	Cold Storage
Temp. Range	-80°C to 110°C
Thickness	2.7 mil (0.0027 in.)
Material	Aluminum
Gas-Permeable	No
UV Transparent	No

 

70534-10

AluminaSeal

100/pk

60.00

HotSeal™

PCR Sealing Film

Film Type	HotSeal™
Ideal Use	PCR
Temp. Range	-40°C to 125°C
Thickness	2.3 mil (0.0023 in.)
Material	Polyester
Gas-Permeable	No
UV Transparent	No

 

70534-20

HotSeal

100/pk

60.00

Microplate Brayer

Uniformly seal your films to microplates with Brayer. It’s perfect for all your sealing films.

70534-40

Microplate Brayer

each

15.00

Breathable Tube Membranes

Micro-permeable Membranes for Microtubes

• Ideal for cell growth and freeze drying.
• Permits consistent gas exhange.
• Easy microtube cap closure.
• Will not bind in centrifuge rotors.
• Easy-to-use finger tab for quick liner removal.
• Pre-cut to fit a variety of popular tube sizes.
• Sterile and free of cytotoxins.

70537-10	Breathe-EASY Membranes	100/pk	36.00
70537-20	Breathe-EASIER Membranes	100/pk	36.00

Cell Culture Plates "PTFE"

Manufactured from pure "PTFE" with round bottom or flat bottom distortion free cavities. Totally inert and may be sterilized by all usual means. Special interest in "PTFE" 96 well culture plates arises from the non-stick nature of "PTFE" and the fact that such plates eliminate adherence of cells to the cavity wall and facilitate sample harvesting.

Specifications:

Cavity Diameter: 6.4mm
Cavity Depth: 10.67mm
Overall Length: 128mm
Overall Width: 85mm
Overall Height: 14mm

Catalog #	Description	Pack	Price
72268-R	Round Bottom	each	140.00
72268-F	Flat Bottom	each	140.00

CC Inserts — Cell Culture Inserts

NUNC’s CC Inserts with Anopore™ membranes or polycarbonate membranes closely mimic an in-vivo environment, resulting in improved cell attachment and growth. The Anapore™ membranes provide maximum clarity for microscopy along with a high porosity, which promotes higher levels of cell differentiation.

Features:

• Transparent when wet: This provides optical clarity when viewing cells either microscopically or photographically. Eliminates background interference for better cell differentiation.
• Inserts with a 0.02μm Anapore™ membrane have maximum clarity for microscopy, are nonauto fluorescent and highly porous.
• High porosity: Ensures free diffusion of nutrients to both basolateral (bottom) and spical plasma (top) membranes of attached cells closely resembling an in-vivo environment.
• Solvent resistant: Cells can be fixed/stained without the need for transferring them
• No matrix coating required: very versatile, no coating needed

NUNC TC Inserts are provided sterilized for use in cell and tissue culture applications. These devices can be used without further treatment or may be protein coated using standard protocols.

The TCI is available in different pore sizes and membrane material.

Typical applications for the TCI are:

• Epithelial polarization studies
• Chemotaxix studies
• Co-culture experiments
• Transport studies
• Electron microscopy
• Toxicity testing
• Modeling of in-vivo barriers.

1. Unique Anapore™ Membrane:

The Anapore™ membrane is a rigid inorganic membrane that is highly transparent when wet and highly porous (40-50% surface porosity). This membrane provides maximum clarity for microscopy, is non-autofluorescent, and highly resistant to most solvents. The Anapore™ membrane can be removed from the housing using the removal tool.

Some typical applications are: epithelial polarization studies; transport studies, toxicity testing and
electron microscopy.

2. Polycarbonate Membrane Inserts:

The polycarbonate membrane does not have the transparent characteristics of the Anapore™ membrane. Due to the large pore sizes available with the polycarbonate membrane, it is particularly suitable for chemotaxix studies. These membranes are easily removed (no need for a removal tool) and are ideal for sectioning for transmission microscopy, using glass or diamond knives.

CC Inserts

TCL Size	10mm	25mm	8 Well Strip
Fits in MultiDish	Multi dish 24	Multi Dish 6	96 Flat Well
Membrane height above base of well (mm)	1.0	1.0	2.0
Culture area (cm2)	0.5	4.2	0.08
Dia. of membrane (mm)	8.0	23.0	3.2 (base l)
Suggested Working Volume, ml*	0.5	1.75	60µl

* Suggested working volume, ml* = In addition to removal well working volume

Anapore® membrane - Sterile

Catalog #	NUNC#	TCI Size	Pore Size	Pore Density /cm2	Pack	Price
72289-12	162243	10mm	0.02µm	1011	12	38.00
72289-48	162243	10mm	0.02µm	1011	48	181
72291-12	162138	25mm	0.02µm	1011	12	52.00
72291-48	162138	25mm	0.02µm	1011	48	178.50
72288-12	136935	10mm	0.2µm	109	12	75.00
72288-48	136935	10mm	0.2µm	109	48	235.00
72290-12	137044	25mm	0.2µm	109	12	76.00
72290-48	137044	25mm	0.2µm	109	48	283.00
72292-15	136730	8 Well Strip	0.2um	109	15	206.00

Polycarbonate Membrane - Sterile

Catalog #	NUNC#	TCI Size	Pore Size	Pore Density /cm2	Pack	Price
72294-12	136781	10mm	0.4µm	1 x 108	12	75.00
72294-48	136781	10mm	0.4µm	1 x 108	48	235.00
72295-12	137060	25mm	0.4µm	1 x 108	12	76.00
72295-48	137060	25mm	0.4µm	1 x 108	48	283.00
72296-12	137370	10mm	3.0µm	2 x 106	12	75.00
72296-48	137370	10mm	3.0µm	2 x 106	48	235.00
72297-12	137435	25mm	3.0µm	2 x 106	12	76.00
72297-48	137435	25mm	3.0µm	2 x 106	48	283.00
72298-12	137443	10mm	8.0µm	1 x 105	12	75.00
72298-48	137443	10mm	8.0µm	1 x 105	48	235.00
72299-12	137508	25mm	8.0µm	1 x 105	12	76.00
72299-48	137508	25mm	8.0µm	1 x 105	48	283.00

Culture Flasks

Special Culture Flasks made from borosilicate glass and "PTFE"

For all applications where gentle stirring is required, such as biological suspensions and micro-carrier cell cultures. The unique stirring action uses a flexible "PTFE" shaft driven through a fully encapsulated rare-earth magnet to give even and complete mixing throughout the liquid. The height of the shaft is adjustable. Contact materials are "PTFE" and borosilicate glass only. The glass vessel has two side-arms with "PTFE" caps and the base is intended to aid mixing. Recommended for use up to 120 rpm max.

Catalog #	Capacity	Neck	S/Arm	Flask HT	Body 0	Price
Unit complete with Vessel, Rotor & two "PTFE" Side Arm Caps
66220-02	250 ml	GL 45	GL 32	150 mm	100 mm	9.00
66220-05	500ml	DL 45	GL 32	190 mm	100 mm	225.00
66220-10	1000 ml	DL 45	GL 32	230 mm	115 mm	272.00
Flask only - no Rotor, no Side Arm Caps
66221-02	250 ml	GL 45	GL 32	150 mm	100 mm	128.00
66221-05	500ml	DL 45	GL 32	190 mm	100 mm	130.00
66221-10	1000 ml	DL 45	GL 32	230 mm	115 mm	159.00
Rotor only (the Rotor can be used with any ISO GL 45 reagent bottle)
66222-02	250 ml	GL 45	----	----	----	60.00
66222-05	500ml	DL 45	----	----	----	60.00
66222-10	1000 ml	DL 45	----	----	----	66.00

Pipettes and Pipette Fillers